AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0594110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594110 MtrunA17_Chr4g0012821 98.750 80 1 0 1 80 1 80 2.17e-51 155
Msa0594110 MtrunA17_Chr8g0379051 85.366 82 10 1 1 80 1 82 4.67e-37 119
Msa0594110 MtrunA17_Chr1g0191471 59.494 79 24 1 10 80 26 104 1.67e-24 88.6
Msa0594110 MtrunA17_Chr2g0326321 60.241 83 27 1 4 80 21 103 9.17e-24 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594110 AT3G60540.2 84.211 76 11 1 3 77 2 77 6.30e-35 114
Msa0594110 AT3G60540.3 84.211 76 11 1 3 77 2 77 6.30e-35 114
Msa0594110 AT3G60540.1 84.211 76 11 1 3 77 2 77 6.30e-35 114
Msa0594110 AT2G45070.1 84.810 79 8 1 3 77 2 80 5.10e-33 109
Msa0594110 AT2G45070.4 84.810 79 8 1 3 77 2 80 5.10e-33 109
Msa0594110 AT2G45070.2 84.810 79 8 1 3 77 2 80 5.10e-33 109
Msa0594110 AT2G45070.3 84.810 79 8 1 3 77 2 80 5.10e-33 109
Msa0594110 AT5G60460.2 58.333 84 27 1 5 80 22 105 1.76e-22 83.6
Msa0594110 AT5G60460.1 58.333 84 27 1 5 80 22 105 1.76e-22 83.6

Find 44 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGCAGCACTTCCTCTTT+GGG 0.197645 4_2:+21168619 None:intergenic
TAGCTGCAGCACTTCCTCTT+TGG 0.251750 4_2:+21168618 None:intergenic
CTTTGTCAGTACTTAGTAAT+AGG 0.295433 4_2:-21168124 Msa0594110:three_prime_UTR
TGTACCTGAATGAGAAATTC+TGG 0.330483 4_2:+21169676 None:intergenic
TCATTTCAGAAAATGGCTTT+AGG 0.333821 4_2:-21168653 Msa0594110:intron
GTAACCAGAATTTCTCATTC+AGG 0.335677 4_2:-21169680 Msa0594110:intron
GGAGCCTCTGGAGGAGCAGC+TGG 0.376820 4_2:-21168560 Msa0594110:CDS
TTCTGGTTACTGAGATTTGT+AGG 0.387656 4_2:+21169693 None:intergenic
ACACCAGGTGGTGGAGCCTC+TGG 0.397039 4_2:-21168572 Msa0594110:CDS
TGTACTTGAGGGAGGCTTAA+GGG 0.407052 4_2:-21168418 Msa0594110:exon
CCTCCAGAGGCTCCACCACC+TGG 0.407875 4_2:+21168569 None:intergenic
GTTGTCATATATGTCATATA+TGG 0.424824 4_2:+21168065 None:intergenic
CTGTACTTGAGGGAGGCTTA+AGG 0.429114 4_2:-21168419 Msa0594110:exon
CAGAAAATGGCTTTAGGTGG+AGG 0.431685 4_2:-21168647 Msa0594110:intron
GGGATGCAAGGCAAGAAGTT+GGG 0.467110 4_2:-21168398 Msa0594110:three_prime_UTR
ATAAAGTATAACGACATATC+AGG 0.468466 4_2:+21168043 None:intergenic
CGTGGGAAAGCTGTACTTGA+GGG 0.469860 4_2:-21168429 Msa0594110:CDS
CTCATCACAAGAACAACATT+AGG 0.475458 4_2:+21168488 None:intergenic
GTTGTTCTTGTGATGAGCAT+TGG 0.493707 4_2:-21168482 Msa0594110:CDS
TGTTGCCGTCCTTCATGTCG+TGG 0.502055 4_2:-21168447 Msa0594110:CDS
AGGGATGCAAGGCAAGAAGT+TGG 0.502672 4_2:-21168399 Msa0594110:three_prime_UTR
GCTGCAGCACTTCCTCTTTG+GGG 0.514244 4_2:+21168620 None:intergenic
AAAACAAATACAATATCAAA+GGG 0.529969 4_2:+21168302 None:intergenic
ATTGTTCCTAGAAGTAAAAG+TGG 0.534496 4_2:-21168211 Msa0594110:three_prime_UTR
CTACTGCAAGCATGAGGAGA+AGG 0.536447 4_2:-21168598 Msa0594110:CDS
AGAAGGAAAACACCAGGTGG+TGG 0.545023 4_2:-21168581 Msa0594110:CDS
GTTGCCGTCCTTCATGTCGT+GGG 0.549428 4_2:-21168446 Msa0594110:CDS
TTTCAGAAAATGGCTTTAGG+TGG 0.554792 4_2:-21168650 Msa0594110:intron
TTACTGAGATTTGTAGGAGT+TGG 0.567416 4_2:+21169699 None:intergenic
GATTAATGCACACTACTACA+TGG 0.588875 4_2:-21168012 Msa0594110:three_prime_UTR
CCAGGTGGTGGAGCCTCTGG+AGG 0.593869 4_2:-21168569 Msa0594110:CDS
TTTAAGTCCAGGTGCATCGT+CGG 0.594963 4_2:+21168517 None:intergenic
AGTTCCAGCTGCTCCTCCAG+AGG 0.601644 4_2:+21168556 None:intergenic
TGTCGTTATACTTTATACAC+AGG 0.604631 4_2:-21168036 Msa0594110:three_prime_UTR
AGGGAGGCTTAAGGGATGCA+AGG 0.606445 4_2:-21168410 Msa0594110:exon
GGTGGAGGAGCTCCCCAAAG+AGG 0.606488 4_2:-21168632 Msa0594110:CDS
ACAGCTTTCCCACGACATGA+AGG 0.608814 4_2:+21168438 None:intergenic
ATGTCATATATGGTTAGCAA+CGG 0.621841 4_2:+21168075 None:intergenic
TCGTGGGAAAGCTGTACTTG+AGG 0.624214 4_2:-21168430 Msa0594110:CDS
CTGCAGCTACTGCAAGCATG+AGG 0.624920 4_2:-21168604 Msa0594110:CDS
ATGAGGAGAAGGAAAACACC+AGG 0.632567 4_2:-21168587 Msa0594110:CDS
CTTTCCCACGACATGAAGGA+CGG 0.643668 4_2:+21168442 None:intergenic
GGGAAAGCTGTACTTGAGGG+AGG 0.648270 4_2:-21168426 Msa0594110:CDS
AGGAGAAGGAAAACACCAGG+TGG 0.731216 4_2:-21168584 Msa0594110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAATTATCTAATTTTTAAT+CGG - chr4_2:21168209-21168228 Msa0594110:three_prime_UTR 10.0%
!!! GTAATTATCTAATTTTTAAT+CGG - chr4_2:21168209-21168228 Msa0594110:three_prime_UTR 10.0%
!!! ATTTTCTGAATTTTGTTTAA+TGG - chr4_2:21168113-21168132 Msa0594110:three_prime_UTR 15.0%
!! AAAACAAATACAATATCAAA+GGG + chr4_2:21169460-21169479 None:intergenic 15.0%
!! AAAAACAAATACAATATCAA+AGG + chr4_2:21169461-21169480 None:intergenic 15.0%
!!! TATTACTTTTAAGTATAACA+AGG - chr4_2:21169501-21169520 Msa0594110:intron 15.0%
!!! ATTACTTTTAAGTATAACAA+GGG - chr4_2:21169502-21169521 Msa0594110:intron 15.0%
!!! ATTTTCTGAATTTTGTTTAA+TGG - chr4_2:21168113-21168132 Msa0594110:three_prime_UTR 15.0%
!! AAAACAAATACAATATCAAA+GGG + chr4_2:21169460-21169479 None:intergenic 15.0%
!! AAAAACAAATACAATATCAA+AGG + chr4_2:21169461-21169480 None:intergenic 15.0%
!!! TATTACTTTTAAGTATAACA+AGG - chr4_2:21169501-21169520 Msa0594110:intron 15.0%
!!! ATTACTTTTAAGTATAACAA+GGG - chr4_2:21169502-21169521 Msa0594110:intron 15.0%
!! TCATCAAAATTTCATAAACT+TGG + chr4_2:21168237-21168256 None:intergenic 20.0%
!!! TGTTTATTTGTTGAATGAAT+TGG - chr4_2:21168608-21168627 Msa0594110:CDS 20.0%
!!! ATTGAGAAACTTTTGTAAAT+TGG - chr4_2:21169037-21169056 Msa0594110:intron 20.0%
!!! ATTGGAAGTTAATTTGAATT+AGG - chr4_2:21169055-21169074 Msa0594110:intron 20.0%
!!! TGTATTTGTTTTTGTTCTTT+TGG - chr4_2:21169468-21169487 Msa0594110:intron 20.0%
!! TCATCAAAATTTCATAAACT+TGG + chr4_2:21168237-21168256 None:intergenic 20.0%
!!! TGTTTATTTGTTGAATGAAT+TGG - chr4_2:21168608-21168627 Msa0594110:CDS 20.0%
!!! ATTGAGAAACTTTTGTAAAT+TGG - chr4_2:21169037-21169056 Msa0594110:intron 20.0%
!!! ATTGGAAGTTAATTTGAATT+AGG - chr4_2:21169055-21169074 Msa0594110:intron 20.0%
!!! TGTATTTGTTTTTGTTCTTT+TGG - chr4_2:21169468-21169487 Msa0594110:intron 20.0%
! TTTCAAATTCCATGCAATAA+AGG - chr4_2:21168163-21168182 Msa0594110:three_prime_UTR 25.0%
! ATCTAAAAACCTTTATTGCA+TGG + chr4_2:21168175-21168194 None:intergenic 25.0%
! AAAACTGTCATAATACTTCT+CGG - chr4_2:21168315-21168334 Msa0594110:three_prime_UTR 25.0%
! AATGATAAGTATCTGACAAA+GGG + chr4_2:21168352-21168371 None:intergenic 25.0%
!! ACAATTCATATCAAGTGTTT+TGG + chr4_2:21168508-21168527 None:intergenic 25.0%
! TCTCATAACAATTTGAAAAC+AGG + chr4_2:21168579-21168598 None:intergenic 25.0%
!!! GAATGAATTGGATTTTGATA+AGG - chr4_2:21168620-21168639 Msa0594110:CDS 25.0%
!! TCGAATATGTCTTTATTTGT+CGG - chr4_2:21168782-21168801 Msa0594110:intron 25.0%
! TGAACTTTCATTTCAGAAAA+TGG - chr4_2:21169099-21169118 Msa0594110:intron 25.0%
! AAAGTGAAAAACAGAAATCT+TGG - chr4_2:21169661-21169680 Msa0594110:intron 25.0%
! GTTGTCATATATGTCATATA+TGG + chr4_2:21169697-21169716 None:intergenic 25.0%
! ATAAAGTATAACGACATATC+AGG + chr4_2:21169719-21169738 None:intergenic 25.0%
! TTTCAAATTCCATGCAATAA+AGG - chr4_2:21168163-21168182 Msa0594110:three_prime_UTR 25.0%
! ATCTAAAAACCTTTATTGCA+TGG + chr4_2:21168175-21168194 None:intergenic 25.0%
! AAAACTGTCATAATACTTCT+CGG - chr4_2:21168315-21168334 Msa0594110:three_prime_UTR 25.0%
! AATGATAAGTATCTGACAAA+GGG + chr4_2:21168352-21168371 None:intergenic 25.0%
!! ACAATTCATATCAAGTGTTT+TGG + chr4_2:21168508-21168527 None:intergenic 25.0%
! TCTCATAACAATTTGAAAAC+AGG + chr4_2:21168579-21168598 None:intergenic 25.0%
!!! GAATGAATTGGATTTTGATA+AGG - chr4_2:21168620-21168639 Msa0594110:CDS 25.0%
!! TCGAATATGTCTTTATTTGT+CGG - chr4_2:21168782-21168801 Msa0594110:intron 25.0%
! TGAACTTTCATTTCAGAAAA+TGG - chr4_2:21169099-21169118 Msa0594110:intron 25.0%
! AAAGTGAAAAACAGAAATCT+TGG - chr4_2:21169661-21169680 Msa0594110:intron 25.0%
! GTTGTCATATATGTCATATA+TGG + chr4_2:21169697-21169716 None:intergenic 25.0%
! ATAAAGTATAACGACATATC+AGG + chr4_2:21169719-21169738 None:intergenic 25.0%
CAATGATAAGTATCTGACAA+AGG + chr4_2:21168353-21168372 None:intergenic 30.0%
! TCATTTTGAATGCAATTCTC+AGG - chr4_2:21168670-21168689 Msa0594110:intron 30.0%
GAATACTAGAAAAATGCTTG+TGG - chr4_2:21168692-21168711 Msa0594110:intron 30.0%
CTTGATATTGTTGAGAATCA+TGG - chr4_2:21168759-21168778 Msa0594110:intron 30.0%
! ATATGTCTTTATTTGTCGGT+TGG - chr4_2:21168786-21168805 Msa0594110:intron 30.0%
TCATTTCAGAAAATGGCTTT+AGG - chr4_2:21169106-21169125 Msa0594110:intron 30.0%
!!! AAGAAGTTGGGTTCATTTTT+TGG - chr4_2:21169373-21169392 Msa0594110:intron 30.0%
ATTGTTCCTAGAAGTAAAAG+TGG - chr4_2:21169548-21169567 Msa0594110:intron 30.0%
!! CTTATACCACTTTTACTTCT+AGG + chr4_2:21169557-21169576 None:intergenic 30.0%
CTTTGTCAGTACTTAGTAAT+AGG - chr4_2:21169635-21169654 Msa0594110:intron 30.0%
ATGTCATATATGGTTAGCAA+CGG + chr4_2:21169687-21169706 None:intergenic 30.0%
TGTCGTTATACTTTATACAC+AGG - chr4_2:21169723-21169742 Msa0594110:five_prime_UTR 30.0%
CAATGATAAGTATCTGACAA+AGG + chr4_2:21168353-21168372 None:intergenic 30.0%
! TCATTTTGAATGCAATTCTC+AGG - chr4_2:21168670-21168689 Msa0594110:intron 30.0%
GAATACTAGAAAAATGCTTG+TGG - chr4_2:21168692-21168711 Msa0594110:intron 30.0%
CTTGATATTGTTGAGAATCA+TGG - chr4_2:21168759-21168778 Msa0594110:intron 30.0%
! ATATGTCTTTATTTGTCGGT+TGG - chr4_2:21168786-21168805 Msa0594110:intron 30.0%
TCATTTCAGAAAATGGCTTT+AGG - chr4_2:21169106-21169125 Msa0594110:intron 30.0%
!!! AAGAAGTTGGGTTCATTTTT+TGG - chr4_2:21169373-21169392 Msa0594110:intron 30.0%
ATTGTTCCTAGAAGTAAAAG+TGG - chr4_2:21169548-21169567 Msa0594110:intron 30.0%
!! CTTATACCACTTTTACTTCT+AGG + chr4_2:21169557-21169576 None:intergenic 30.0%
CTTTGTCAGTACTTAGTAAT+AGG - chr4_2:21169635-21169654 Msa0594110:intron 30.0%
ATGTCATATATGGTTAGCAA+CGG + chr4_2:21169687-21169706 None:intergenic 30.0%
TGTCGTTATACTTTATACAC+AGG - chr4_2:21169723-21169742 Msa0594110:five_prime_UTR 30.0%
TTACTGAGATTTGTAGGAGT+TGG + chr4_2:21168063-21168082 None:intergenic 35.0%
! TTCTGGTTACTGAGATTTGT+AGG + chr4_2:21168069-21168088 None:intergenic 35.0%
GTAACCAGAATTTCTCATTC+AGG - chr4_2:21168079-21168098 Msa0594110:three_prime_UTR 35.0%
TGTACCTGAATGAGAAATTC+TGG + chr4_2:21168086-21168105 None:intergenic 35.0%
GTCATAATACTTCTCGGATT+AGG - chr4_2:21168321-21168340 Msa0594110:three_prime_UTR 35.0%
GATATTGACATGTTTGTGTC+TGG - chr4_2:21168462-21168481 Msa0594110:CDS 35.0%
!! TAAGGTATTGCATTTGCATG+TGG - chr4_2:21168638-21168657 Msa0594110:CDS 35.0%
!! AAGGTATTGCATTTGCATGT+GGG - chr4_2:21168639-21168658 Msa0594110:CDS 35.0%
! TTGTCGGTTGGATAGAAATA+TGG - chr4_2:21168798-21168817 Msa0594110:intron 35.0%
ATGTCGTTGTTTGATGAATG+TGG - chr4_2:21168837-21168856 Msa0594110:intron 35.0%
!! TTTCAGAAAATGGCTTTAGG+TGG - chr4_2:21169109-21169128 Msa0594110:intron 35.0%
!! TTAGGTGAGATTTTAAGTCC+AGG + chr4_2:21169256-21169275 None:intergenic 35.0%
CTCATCACAAGAACAACATT+AGG + chr4_2:21169274-21169293 None:intergenic 35.0%
GATTAATGCACACTACTACA+TGG - chr4_2:21169747-21169766 Msa0594110:five_prime_UTR 35.0%
TTACTGAGATTTGTAGGAGT+TGG + chr4_2:21168063-21168082 None:intergenic 35.0%
! TTCTGGTTACTGAGATTTGT+AGG + chr4_2:21168069-21168088 None:intergenic 35.0%
GTAACCAGAATTTCTCATTC+AGG - chr4_2:21168079-21168098 Msa0594110:three_prime_UTR 35.0%
TGTACCTGAATGAGAAATTC+TGG + chr4_2:21168086-21168105 None:intergenic 35.0%
GTCATAATACTTCTCGGATT+AGG - chr4_2:21168321-21168340 Msa0594110:three_prime_UTR 35.0%
GATATTGACATGTTTGTGTC+TGG - chr4_2:21168462-21168481 Msa0594110:CDS 35.0%
!! TAAGGTATTGCATTTGCATG+TGG - chr4_2:21168638-21168657 Msa0594110:CDS 35.0%
!! AAGGTATTGCATTTGCATGT+GGG - chr4_2:21168639-21168658 Msa0594110:CDS 35.0%
! TTGTCGGTTGGATAGAAATA+TGG - chr4_2:21168798-21168817 Msa0594110:intron 35.0%
ATGTCGTTGTTTGATGAATG+TGG - chr4_2:21168837-21168856 Msa0594110:intron 35.0%
!! TTTCAGAAAATGGCTTTAGG+TGG - chr4_2:21169109-21169128 Msa0594110:intron 35.0%
!! TTAGGTGAGATTTTAAGTCC+AGG + chr4_2:21169256-21169275 None:intergenic 35.0%
CTCATCACAAGAACAACATT+AGG + chr4_2:21169274-21169293 None:intergenic 35.0%
GATTAATGCACACTACTACA+TGG - chr4_2:21169747-21169766 Msa0594110:five_prime_UTR 35.0%
ACGTCTTATCCCACTAAGTA+AGG + chr4_2:21168867-21168886 None:intergenic 40.0%
GTTGTTCTTGTGATGAGCAT+TGG - chr4_2:21169277-21169296 Msa0594110:intron 40.0%
ACGTCTTATCCCACTAAGTA+AGG + chr4_2:21168867-21168886 None:intergenic 40.0%
GTTGTTCTTGTGATGAGCAT+TGG - chr4_2:21169277-21169296 Msa0594110:intron 40.0%
GACATGTTTGTGTCTGGTGT+CGG - chr4_2:21168468-21168487 Msa0594110:CDS 45.0%
ATGTGGCTGACCTTACTTAG+TGG - chr4_2:21168854-21168873 Msa0594110:intron 45.0%
TGTGGCTGACCTTACTTAGT+GGG - chr4_2:21168855-21168874 Msa0594110:intron 45.0%
!! CAGAAAATGGCTTTAGGTGG+AGG - chr4_2:21169112-21169131 Msa0594110:intron 45.0%
ATGAGGAGAAGGAAAACACC+AGG - chr4_2:21169172-21169191 Msa0594110:intron 45.0%
TTTAAGTCCAGGTGCATCGT+CGG + chr4_2:21169245-21169264 None:intergenic 45.0%
TGTACTTGAGGGAGGCTTAA+GGG - chr4_2:21169341-21169360 Msa0594110:intron 45.0%
GACATGTTTGTGTCTGGTGT+CGG - chr4_2:21168468-21168487 Msa0594110:CDS 45.0%
ATGTGGCTGACCTTACTTAG+TGG - chr4_2:21168854-21168873 Msa0594110:intron 45.0%
TGTGGCTGACCTTACTTAGT+GGG - chr4_2:21168855-21168874 Msa0594110:intron 45.0%
!! CAGAAAATGGCTTTAGGTGG+AGG - chr4_2:21169112-21169131 Msa0594110:intron 45.0%
ATGAGGAGAAGGAAAACACC+AGG - chr4_2:21169172-21169191 Msa0594110:intron 45.0%
TTTAAGTCCAGGTGCATCGT+CGG + chr4_2:21169245-21169264 None:intergenic 45.0%
TGTACTTGAGGGAGGCTTAA+GGG - chr4_2:21169341-21169360 Msa0594110:intron 45.0%
ATCTTAAACCGCGACTGCGA+CGG + chr4_2:21168294-21168313 None:intergenic 50.0%
TCGTGTACTCGTCGTGTTCA+CGG + chr4_2:21168388-21168407 None:intergenic 50.0%
AGCTGCAGCACTTCCTCTTT+GGG + chr4_2:21169143-21169162 None:intergenic 50.0%
TAGCTGCAGCACTTCCTCTT+TGG + chr4_2:21169144-21169163 None:intergenic 50.0%
CTACTGCAAGCATGAGGAGA+AGG - chr4_2:21169161-21169180 Msa0594110:intron 50.0%
AGGAGAAGGAAAACACCAGG+TGG - chr4_2:21169175-21169194 Msa0594110:intron 50.0%
AGAAGGAAAACACCAGGTGG+TGG - chr4_2:21169178-21169197 Msa0594110:intron 50.0%
! TTTTACACCGACGATGCACC+TGG - chr4_2:21169235-21169254 Msa0594110:intron 50.0%
CTTTCCCACGACATGAAGGA+CGG + chr4_2:21169320-21169339 None:intergenic 50.0%
ACAGCTTTCCCACGACATGA+AGG + chr4_2:21169324-21169343 None:intergenic 50.0%
TCGTGGGAAAGCTGTACTTG+AGG - chr4_2:21169329-21169348 Msa0594110:intron 50.0%
CGTGGGAAAGCTGTACTTGA+GGG - chr4_2:21169330-21169349 Msa0594110:intron 50.0%
CTGTACTTGAGGGAGGCTTA+AGG - chr4_2:21169340-21169359 Msa0594110:intron 50.0%
AGGGATGCAAGGCAAGAAGT+TGG - chr4_2:21169360-21169379 Msa0594110:intron 50.0%
GGGATGCAAGGCAAGAAGTT+GGG - chr4_2:21169361-21169380 Msa0594110:intron 50.0%
ATCTTAAACCGCGACTGCGA+CGG + chr4_2:21168294-21168313 None:intergenic 50.0%
TCGTGTACTCGTCGTGTTCA+CGG + chr4_2:21168388-21168407 None:intergenic 50.0%
AGCTGCAGCACTTCCTCTTT+GGG + chr4_2:21169143-21169162 None:intergenic 50.0%
TAGCTGCAGCACTTCCTCTT+TGG + chr4_2:21169144-21169163 None:intergenic 50.0%
CTACTGCAAGCATGAGGAGA+AGG - chr4_2:21169161-21169180 Msa0594110:intron 50.0%
AGGAGAAGGAAAACACCAGG+TGG - chr4_2:21169175-21169194 Msa0594110:intron 50.0%
AGAAGGAAAACACCAGGTGG+TGG - chr4_2:21169178-21169197 Msa0594110:intron 50.0%
! TTTTACACCGACGATGCACC+TGG - chr4_2:21169235-21169254 Msa0594110:intron 50.0%
CTTTCCCACGACATGAAGGA+CGG + chr4_2:21169320-21169339 None:intergenic 50.0%
ACAGCTTTCCCACGACATGA+AGG + chr4_2:21169324-21169343 None:intergenic 50.0%
TCGTGGGAAAGCTGTACTTG+AGG - chr4_2:21169329-21169348 Msa0594110:intron 50.0%
CGTGGGAAAGCTGTACTTGA+GGG - chr4_2:21169330-21169349 Msa0594110:intron 50.0%
CTGTACTTGAGGGAGGCTTA+AGG - chr4_2:21169340-21169359 Msa0594110:intron 50.0%
AGGGATGCAAGGCAAGAAGT+TGG - chr4_2:21169360-21169379 Msa0594110:intron 50.0%
GGGATGCAAGGCAAGAAGTT+GGG - chr4_2:21169361-21169380 Msa0594110:intron 50.0%
TCTTAAACCGCGACTGCGAC+GGG + chr4_2:21168293-21168312 None:intergenic 55.0%
ACTCGTCGTGTTCACGGTGT+TGG + chr4_2:21168382-21168401 None:intergenic 55.0%
GCTGCAGCACTTCCTCTTTG+GGG + chr4_2:21169142-21169161 None:intergenic 55.0%
CTGCAGCTACTGCAAGCATG+AGG - chr4_2:21169155-21169174 Msa0594110:intron 55.0%
TGTTGCCGTCCTTCATGTCG+TGG - chr4_2:21169312-21169331 Msa0594110:intron 55.0%
GTTGCCGTCCTTCATGTCGT+GGG - chr4_2:21169313-21169332 Msa0594110:intron 55.0%
GGGAAAGCTGTACTTGAGGG+AGG - chr4_2:21169333-21169352 Msa0594110:intron 55.0%
AGGGAGGCTTAAGGGATGCA+AGG - chr4_2:21169349-21169368 Msa0594110:intron 55.0%
TCTTAAACCGCGACTGCGAC+GGG + chr4_2:21168293-21168312 None:intergenic 55.0%
ACTCGTCGTGTTCACGGTGT+TGG + chr4_2:21168382-21168401 None:intergenic 55.0%
GCTGCAGCACTTCCTCTTTG+GGG + chr4_2:21169142-21169161 None:intergenic 55.0%
CTGCAGCTACTGCAAGCATG+AGG - chr4_2:21169155-21169174 Msa0594110:intron 55.0%
TGTTGCCGTCCTTCATGTCG+TGG - chr4_2:21169312-21169331 Msa0594110:intron 55.0%
GTTGCCGTCCTTCATGTCGT+GGG - chr4_2:21169313-21169332 Msa0594110:intron 55.0%
GGGAAAGCTGTACTTGAGGG+AGG - chr4_2:21169333-21169352 Msa0594110:intron 55.0%
AGGGAGGCTTAAGGGATGCA+AGG - chr4_2:21169349-21169368 Msa0594110:intron 55.0%
CTTAAACCGCGACTGCGACG+GGG + chr4_2:21168292-21168311 None:intergenic 60.0%
AGTTCCAGCTGCTCCTCCAG+AGG + chr4_2:21169206-21169225 None:intergenic 60.0%
CTTAAACCGCGACTGCGACG+GGG + chr4_2:21168292-21168311 None:intergenic 60.0%
AGTTCCAGCTGCTCCTCCAG+AGG + chr4_2:21169206-21169225 None:intergenic 60.0%
GGTGGAGGAGCTCCCCAAAG+AGG - chr4_2:21169127-21169146 Msa0594110:intron 65.0%
!! ACACCAGGTGGTGGAGCCTC+TGG - chr4_2:21169187-21169206 Msa0594110:intron 65.0%
GGTGGAGGAGCTCCCCAAAG+AGG - chr4_2:21169127-21169146 Msa0594110:intron 65.0%
!! ACACCAGGTGGTGGAGCCTC+TGG - chr4_2:21169187-21169206 Msa0594110:intron 65.0%
ACGTGTCCCCGTCGCAGTCG+CGG - chr4_2:21168283-21168302 Msa0594110:three_prime_UTR 70.0%
CCTCCAGAGGCTCCACCACC+TGG + chr4_2:21169193-21169212 None:intergenic 70.0%
!! CCAGGTGGTGGAGCCTCTGG+AGG - chr4_2:21169190-21169209 Msa0594110:intron 70.0%
! GGAGCCTCTGGAGGAGCAGC+TGG - chr4_2:21169199-21169218 Msa0594110:intron 70.0%
ACGTGTCCCCGTCGCAGTCG+CGG - chr4_2:21168283-21168302 Msa0594110:three_prime_UTR 70.0%
CCTCCAGAGGCTCCACCACC+TGG + chr4_2:21169193-21169212 None:intergenic 70.0%
!! CCAGGTGGTGGAGCCTCTGG+AGG - chr4_2:21169190-21169209 Msa0594110:intron 70.0%
! GGAGCCTCTGGAGGAGCAGC+TGG - chr4_2:21169199-21169218 Msa0594110:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 21167996 21169785 21167996 ID=Msa0594110;Name=Msa0594110
chr4_2 mRNA 21167996 21169785 21167996 ID=Msa0594110-mRNA-1;Parent=Msa0594110;Name=Msa0594110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105|0|0.5|1|0|0.5|2|425|80
chr4_2 exon 21167996 21168663 21167996 ID=Msa0594110-mRNA-1:exon:5920;Parent=Msa0594110-mRNA-1
chr4_2 exon 21169681 21169785 21169681 ID=Msa0594110-mRNA-1:exon:5919;Parent=Msa0594110-mRNA-1
chr4_2 five_prime_UTR 21169681 21169785 21169681 ID=Msa0594110-mRNA-1:five_prime_utr;Parent=Msa0594110-mRNA-1
chr4_2 CDS 21168421 21168663 21168421 ID=Msa0594110-mRNA-1:cds;Parent=Msa0594110-mRNA-1
chr4_2 three_prime_UTR 21167996 21168420 21167996 ID=Msa0594110-mRNA-1:three_prime_utr;Parent=Msa0594110-mRNA-1
Gene Sequence

>Msa0594110

ATGGCTTTAGGTGGAGGAGCTCCCCAAAGAGGAAGTGCTGCAGCTACTGCAAGCATGAGGAGAAGGAAAACACCAGGTGGTGGAGCCTCTGGAGGAGCAGCTGGAACTATGCTTCAATTTTACACCGACGATGCACCTGGACTTAAAATCTCACCTAATGTTGTTCTTGTGATGAGCATTGGCTTTATAGCATTTGTTGCCGTCCTTCATGTCGTGGGAAAGCTGTACTTGAGGGAGGCTTAA
Protein sequence

>Msa0594110

MALGGGAPQRGSAAATASMRRRKTPGGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSIGFIAFVAVLHVVGKLYLREA*