AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0532200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0532200 MtrunA17_Chr4g0005491 96.341 410 15 0 1 410 1 410 0.0 830
Msa0532200 MtrunA17_Chr7g0243301 78.431 408 85 1 6 410 5 412 0.0 687
Msa0532200 MtrunA17_Chr4g0005481 55.096 363 153 4 45 407 94 446 1.22e-145 421
Msa0532200 MtrunA17_Chr3g0114101 51.862 349 162 3 62 409 89 432 1.60e-133 390
Msa0532200 MtrunA17_Chr5g0437601 51.576 349 163 3 62 409 83 426 6.34e-133 388
Msa0532200 MtrunA17_Chr8g0360521 46.384 401 194 7 21 408 18 410 3.24e-125 368
Msa0532200 MtrunA17_Chr3g0113671 49.284 349 169 5 62 407 106 449 1.90e-121 360
Msa0532200 MtrunA17_Chr4g0006991 46.000 350 179 6 61 407 85 427 7.06e-111 332
Msa0532200 MtrunA17_Chr5g0437291 45.070 355 184 6 62 410 165 514 1.96e-109 331
Msa0532200 MtrunA17_Chr7g0242701 45.845 349 181 5 61 407 85 427 4.77e-105 317
Msa0532200 MtrunA17_Chr1g0175671 39.335 361 199 8 57 410 165 512 9.32e-87 273
Msa0532200 MtrunA17_Chr3g0105301 33.884 363 220 7 51 409 32 378 1.87e-81 254
Msa0532200 MtrunA17_Chr4g0068111 37.119 361 205 8 56 408 108 454 1.04e-80 256
Msa0532200 MtrunA17_Chr7g0258851 37.673 361 205 8 57 410 243 590 1.27e-80 259
Msa0532200 MtrunA17_Chr5g0430371 35.457 361 207 7 60 409 56 401 9.71e-80 251
Msa0532200 MtrunA17_Chr5g0413271 38.571 350 197 8 61 408 144 477 1.65e-79 253
Msa0532200 MtrunA17_Chr1g0210771 36.467 351 201 9 57 407 54 382 1.07e-78 248
Msa0532200 MtrunA17_Chr1g0171171 36.857 350 198 9 58 407 49 375 4.65e-78 246
Msa0532200 MtrunA17_Chr8g0368461 37.831 378 202 9 50 408 166 529 6.17e-78 250
Msa0532200 MtrunA17_Chr7g0269991 38.764 356 189 9 62 410 137 470 2.03e-75 242
Msa0532200 MtrunA17_Chr3g0097891 35.171 381 212 10 25 405 3 348 3.88e-75 238
Msa0532200 MtrunA17_Chr2g0287901 37.260 365 194 9 56 405 91 435 1.42e-74 239
Msa0532200 MtrunA17_Chr2g0314801 35.677 384 207 11 26 407 8 353 3.09e-73 233
Msa0532200 MtrunA17_Chr7g0259711 35.870 368 206 8 56 410 85 435 4.72e-73 235
Msa0532200 MtrunA17_Chr4g0045941 35.694 353 199 9 55 407 26 350 5.35e-73 232
Msa0532200 MtrunA17_Chr3g0085081 35.920 348 191 8 62 407 31 348 2.45e-72 230
Msa0532200 MtrunA17_Chr3g0091031 35.457 361 197 9 49 407 22 348 1.18e-71 228
Msa0532200 MtrunA17_Chr4g0045951 32.225 391 236 10 17 407 1 362 4.45e-71 228
Msa0532200 MtrunA17_Chr1g0213281 34.278 353 217 7 62 409 175 517 1.19e-70 231
Msa0532200 MtrunA17_Chr3g0085071 34.262 359 204 8 49 407 37 363 1.24e-70 226
Msa0532200 MtrunA17_Chr4g0071391 35.376 359 206 10 58 407 105 446 5.94e-70 227
Msa0532200 MtrunA17_Chr5g0413851 35.260 346 196 11 62 407 42 359 8.18e-69 221
Msa0532200 MtrunA17_Chr4g0041011 32.471 348 206 8 62 409 47 365 2.16e-65 213
Msa0532200 MtrunA17_Chr2g0283581 33.526 346 201 10 62 407 42 358 6.99e-65 211
Msa0532200 MtrunA17_Chr2g0325481 32.857 350 204 9 60 408 48 367 1.65e-64 211
Msa0532200 MtrunA17_Chr2g0314791 31.486 397 214 12 19 408 1 346 8.13e-64 208
Msa0532200 MtrunA17_Chr3g0126021 30.831 373 219 9 50 410 117 462 1.04e-62 209
Msa0532200 MtrunA17_Chr8g0384151 29.494 356 220 7 59 408 87 417 2.77e-57 194
Msa0532200 MtrunA17_Chr6g0456681 32.958 355 211 11 62 403 64 404 2.42e-46 164
Msa0532200 MtrunA17_Chr8g0347321 30.168 358 219 15 61 403 94 435 4.23e-42 153
Msa0532200 MtrunA17_Chr5g0414061 31.793 368 210 13 60 407 92 438 8.15e-42 153
Msa0532200 MtrunA17_Chr6g0476041 28.919 370 235 12 53 407 158 514 3.29e-41 152
Msa0532200 MtrunA17_Chr5g0414091 30.194 361 215 14 62 403 85 427 8.49e-41 150
Msa0532200 MtrunA17_Chr6g0456691 30.914 372 220 14 49 403 64 415 1.06e-40 149
Msa0532200 MtrunA17_Chr7g0226991 30.952 378 222 15 46 403 40 398 1.72e-40 148
Msa0532200 MtrunA17_Chr1g0209921 30.242 248 162 5 48 294 30 267 8.24e-40 143
Msa0532200 MtrunA17_Chr4g0070341 30.303 363 213 14 62 407 132 471 9.12e-40 147
Msa0532200 MtrunA17_Chr1g0188191 28.418 373 219 13 60 407 232 581 1.63e-39 149
Msa0532200 MtrunA17_Chr5g0414041 32.361 377 209 16 50 403 76 429 8.92e-38 142
Msa0532200 MtrunA17_Chr8g0391481 31.198 359 215 16 61 403 26 368 3.73e-37 139
Msa0532200 MtrunA17_Chr8g0384421 28.312 385 219 15 50 407 118 472 4.91e-37 140
Msa0532200 MtrunA17_Chr5g0414051 29.670 364 212 17 62 403 75 416 1.31e-35 135
Msa0532200 MtrunA17_Chr1g0178081 35.545 211 123 5 49 248 42 250 2.25e-35 133
Msa0532200 MtrunA17_Chr1g0178081 39.815 108 55 2 303 410 247 344 2.90e-16 79.7
Msa0532200 MtrunA17_Chr3g0140371 30.769 364 205 18 62 403 18 356 6.46e-35 132
Msa0532200 MtrunA17_Chr8g0387921 26.230 366 226 15 60 403 72 415 4.51e-34 131
Msa0532200 MtrunA17_Chr4g0049421 29.396 381 198 17 62 407 48 392 5.54e-34 130
Msa0532200 MtrunA17_Chr2g0283851 28.249 354 228 12 63 403 56 396 1.67e-29 118
Msa0532200 MtrunA17_Chr6g0451321 25.479 365 230 11 62 407 116 457 1.63e-28 116
Msa0532200 MtrunA17_Chr6g0455901 29.032 372 212 16 62 407 71 416 1.76e-27 113
Msa0532200 MtrunA17_Chr7g0221271 37.063 143 79 3 53 191 70 205 2.41e-24 99.8
Msa0532200 MtrunA17_Chr7g0221291 43.434 99 55 1 53 151 70 167 2.81e-23 95.9
Msa0532200 MtrunA17_Chr2g0287931 37.903 124 68 3 123 239 2 123 1.15e-19 84.3
Msa0532200 MtrunA17_Chr3g0085101 37.963 108 62 2 259 366 4 106 1.57e-17 78.2
Msa0532200 MtrunA17_Chr2g0287911 43.590 78 34 3 41 118 42 109 5.30e-14 67.8
Msa0532200 MtrunA17_Chr5g0414081 37.121 132 70 6 277 403 72 195 7.93e-13 67.4
Msa0532200 MtrunA17_Chr5g0429191 57.447 47 17 1 354 400 2 45 1.65e-12 61.6
Msa0532200 MtrunA17_Chr1g0209931 40.000 80 34 2 329 408 28 93 2.11e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0532200 AT2G38320.1 65.578 398 130 3 17 408 9 405 0.0 549
Msa0532200 AT2G38320.2 69.969 323 95 2 87 408 1 322 4.80e-171 481
Msa0532200 AT5G01620.2 55.460 348 147 4 61 407 109 449 2.30e-143 416
Msa0532200 AT5G01620.1 55.460 348 147 4 61 407 109 449 2.30e-143 416
Msa0532200 AT5G01620.3 55.460 348 147 4 61 407 117 457 3.45e-143 416
Msa0532200 AT2G40320.1 51.576 349 163 3 62 409 81 424 2.14e-132 387
Msa0532200 AT2G40150.1 49.582 359 167 5 61 410 70 423 3.43e-127 374
Msa0532200 AT3G11030.1 50.000 350 168 4 62 409 106 450 1.56e-126 373
Msa0532200 AT3G55990.1 49.575 353 170 5 61 410 139 486 2.87e-123 366
Msa0532200 AT1G73140.1 50.284 352 164 6 62 410 67 410 2.62e-122 361
Msa0532200 AT5G01360.2 45.272 349 184 4 61 407 35 378 1.55e-112 335
Msa0532200 AT5G01360.1 45.272 349 184 4 61 407 91 434 6.79e-112 335
Msa0532200 AT2G40160.2 45.480 354 184 7 62 410 77 426 1.91e-108 327
Msa0532200 AT2G40160.1 45.480 354 184 7 62 410 77 426 4.95e-108 325
Msa0532200 AT5G06700.1 40.278 360 189 10 56 405 249 592 4.93e-83 266
Msa0532200 AT5G58600.1 37.006 354 204 7 60 409 64 402 2.07e-82 258
Msa0532200 AT3G12060.1 38.205 390 217 10 28 408 162 536 5.25e-82 262
Msa0532200 AT3G54260.1 35.606 396 223 10 22 408 3 375 8.32e-82 256
Msa0532200 AT5G49340.1 37.270 381 219 9 37 408 73 442 1.25e-80 255
Msa0532200 AT1G60790.1 35.385 390 234 8 25 408 156 533 1.87e-79 255
Msa0532200 AT3G62390.1 35.768 397 230 9 20 410 97 474 1.51e-78 251
Msa0532200 AT5G19160.1 37.808 365 195 8 57 405 94 442 1.60e-78 250
Msa0532200 AT3G06080.2 38.889 360 195 10 57 405 98 443 3.26e-78 249
Msa0532200 AT2G40160.3 48.319 238 117 5 62 295 77 312 4.26e-76 239
Msa0532200 AT1G78710.1 34.091 352 205 8 57 408 33 357 5.46e-75 238
Msa0532200 AT2G30010.1 36.565 361 202 11 60 409 54 398 5.82e-75 239
Msa0532200 AT2G30010.2 36.565 361 202 11 60 409 97 441 9.73e-75 239
Msa0532200 AT1G29050.1 33.155 374 221 8 34 407 34 378 1.47e-74 237
Msa0532200 AT5G20590.1 36.915 363 200 9 57 408 136 480 6.37e-74 239
Msa0532200 AT2G34070.1 34.384 349 200 9 60 407 63 383 8.06e-72 230
Msa0532200 AT3G14850.2 35.043 351 203 8 57 407 29 354 1.07e-71 229
Msa0532200 AT2G30900.1 35.043 351 199 10 62 409 43 367 8.72e-71 227
Msa0532200 AT5G06230.2 35.753 372 209 8 49 408 12 365 1.18e-70 227
Msa0532200 AT5G06230.1 35.753 372 209 8 49 408 53 406 3.22e-70 227
Msa0532200 AT3G11570.1 33.416 404 229 11 27 408 35 420 2.41e-68 223
Msa0532200 AT2G31110.2 33.505 388 229 9 21 407 3 362 4.19e-66 215
Msa0532200 AT2G42570.1 32.378 349 208 9 60 408 45 365 8.96e-64 209
Msa0532200 AT1G48880.1 31.852 405 234 10 19 410 67 442 4.99e-60 201
Msa0532200 AT5G19160.2 39.754 244 141 2 57 295 94 336 8.84e-59 196
Msa0532200 AT3G06080.1 40.081 247 136 5 57 295 98 340 2.49e-55 186
Msa0532200 AT1G78710.2 30.966 352 163 10 57 408 26 297 2.76e-55 185
Msa0532200 AT5G58600.2 38.397 237 136 5 60 295 64 291 8.06e-53 178
Msa0532200 AT3G62390.2 35.106 282 174 5 20 296 97 374 1.23e-51 179
Msa0532200 AT5G20590.2 36.400 250 141 4 57 295 136 378 2.48e-47 166
Msa0532200 AT5G06230.3 34.884 258 152 4 49 295 53 305 7.86e-46 160
Msa0532200 AT3G14850.1 33.455 275 159 7 133 407 1 251 2.88e-45 157
Msa0532200 AT2G34070.2 32.246 276 159 8 133 407 1 249 3.66e-45 157
Msa0532200 AT5G64020.1 30.458 371 214 16 62 407 57 408 1.40e-42 154
Msa0532200 AT5G20680.6 29.839 372 203 12 62 407 118 457 3.64e-42 154
Msa0532200 AT2G30900.2 34.071 226 139 5 62 286 43 259 5.13e-42 149
Msa0532200 AT5G20680.2 29.839 372 203 12 62 407 194 533 1.34e-41 154
Msa0532200 AT5G20680.4 29.839 372 203 12 62 407 212 551 1.65e-41 154
Msa0532200 AT5G20680.1 29.839 372 203 12 62 407 212 551 1.65e-41 154
Msa0532200 AT5G20680.3 29.839 372 203 12 62 407 212 551 1.65e-41 154
Msa0532200 AT5G20680.5 29.839 372 203 12 62 407 212 551 1.65e-41 154
Msa0532200 AT1G01430.1 29.101 378 232 15 53 410 89 450 3.99e-41 151
Msa0532200 AT2G31110.3 32.210 267 170 5 21 286 3 259 4.01e-41 147
Msa0532200 AT3G02440.1 30.194 361 210 12 62 403 127 464 4.72e-41 152
Msa0532200 AT3G02440.2 30.194 361 210 12 62 403 135 472 5.14e-41 152
Msa0532200 AT2G37720.1 29.428 367 211 16 62 407 143 482 6.23e-40 149
Msa0532200 AT5G51640.1 27.937 383 234 14 49 407 124 488 6.40e-40 149
Msa0532200 AT4G25360.1 28.084 381 222 12 55 407 168 524 1.07e-39 149
Msa0532200 AT4G25360.2 28.084 381 222 12 55 407 168 524 1.07e-39 149
Msa0532200 AT1G70230.1 28.169 355 216 10 62 403 79 407 3.75e-39 145
Msa0532200 AT5G64470.3 30.319 376 220 16 52 408 43 395 9.14e-39 144
Msa0532200 AT5G15890.1 28.912 377 214 14 49 403 174 518 2.51e-38 145
Msa0532200 AT5G64470.2 29.211 380 225 15 52 408 43 401 1.25e-37 141
Msa0532200 AT4G01080.1 28.431 408 244 15 23 410 54 433 9.12e-37 139
Msa0532200 AT4G23790.1 28.378 370 226 17 59 407 76 427 8.89e-36 136
Msa0532200 AT5G15900.1 27.206 408 259 13 19 407 21 409 2.64e-35 135
Msa0532200 AT4G11090.1 26.400 375 225 16 59 407 76 425 3.54e-35 135
Msa0532200 AT5G15900.2 27.206 408 259 13 19 407 40 428 3.56e-35 135
Msa0532200 AT2G14530.1 26.703 367 230 12 60 407 60 406 7.13e-34 131
Msa0532200 AT2G31110.1 33.190 232 130 6 174 405 6 212 1.64e-32 122
Msa0532200 AT3G28150.1 25.659 417 262 15 13 407 20 410 3.50e-32 126
Msa0532200 AT5G64470.4 28.947 266 163 12 51 299 42 298 1.01e-21 95.5
Msa0532200 AT5G64470.1 29.057 265 162 12 52 299 43 298 1.42e-21 95.1

Find 113 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGATTCTGATTCCTAAAT+TGG 0.167939 4_1:+6223447 None:intergenic
ATGCAATCTGGATAAGATTT+TGG 0.178271 4_1:+6220997 None:intergenic
GTTCCATTTATCTTTGGTTT+TGG 0.244957 4_1:-6220869 Msa0532200:three_prime_UTR
TATCAAATGTTTGTCATCTA+TGG 0.258736 4_1:+6220914 None:intergenic
GATATTTGCTCTTCTGTTAG+AGG 0.262790 4_1:+6221024 None:intergenic
CCGTCCTATCTGCCACCTTA+TGG 0.266201 4_1:+6222175 None:intergenic
GTGAATGCAAATGGCAATTA+AGG 0.273416 4_1:-6223494 Msa0532200:exon
AAACCAAAACCAAAGATAAA+TGG 0.277070 4_1:+6220866 None:intergenic
GGGTCTTGTTACGATTAATA+TGG 0.277488 4_1:+6221388 None:intergenic
ACCTTTCTGTATACTCCATT+AGG 0.286912 4_1:+6221468 None:intergenic
CAATATGTTCCATTTATCTT+TGG 0.290497 4_1:-6220875 Msa0532200:three_prime_UTR
TCTGCCACCTTATGGTTTAC+TGG 0.295188 4_1:+6222183 None:intergenic
CATTGCTTCCTATATATTGA+TGG 0.315991 4_1:+6221051 None:intergenic
ATGCAACCATCGAGTTCTAT+TGG 0.320185 4_1:-6222239 Msa0532200:CDS
TCAAAATGTGACTTATTCTA+TGG 0.322368 4_1:-6223313 Msa0532200:CDS
AATTTGATTCTACAAGTAAT+GGG 0.332243 4_1:+6222214 None:intergenic
TCCTTCTTGTGTTAGATATA+TGG 0.335216 4_1:+6223378 None:intergenic
GTAGTGTTACACACCAATTT+AGG 0.336212 4_1:-6223460 Msa0532200:CDS
CCTTTCTGTATACTCCATTA+GGG 0.337001 4_1:+6221469 None:intergenic
AGAAGCTCATTCCAAGTATC+AGG 0.342301 4_1:+6220952 None:intergenic
GAGGCTTCCACCTCCAATTC+TGG 0.343204 4_1:+6223182 None:intergenic
TCCCTGGTGTGCCTGATACT+TGG 0.347343 4_1:-6220963 Msa0532200:CDS
CAGTTGGCTTGTGTGAAGTT+TGG 0.347389 4_1:-6223223 Msa0532200:CDS
ACAAGAGGCTTGTGTTTGTT+GGG 0.354831 4_1:-6222654 Msa0532200:CDS
CTTATCCAGATTGCATTCAT+TGG 0.361304 4_1:-6220990 Msa0532200:CDS
ATGAAATGGCCTTGAGGACT+TGG 0.387426 4_1:-6221428 Msa0532200:CDS
TGGTGTGTAACACTACTATT+TGG 0.394186 4_1:+6223467 None:intergenic
CAGAGGACAATGGGTTTCAA+TGG 0.399093 4_1:-6222620 Msa0532200:CDS
AACAAGAGGCTTGTGTTTGT+TGG 0.403969 4_1:-6222655 Msa0532200:CDS
GAATTTGATTCTACAAGTAA+TGG 0.406884 4_1:+6222213 None:intergenic
AGGATCTTAGCTACCAGAAT+TGG 0.416193 4_1:-6223195 Msa0532200:CDS
AAAGAGGGATATAGTGGAAA+TGG 0.421516 4_1:-6221192 Msa0532200:CDS
TTGTCATCTATGGAAAATAT+AGG 0.423330 4_1:+6220924 None:intergenic
TGCAACCATCGAGTTCTATT+GGG 0.434606 4_1:-6222238 Msa0532200:CDS
ATCACAAAAGAGGGATATAG+TGG 0.437384 4_1:-6221198 Msa0532200:CDS
TTCACTCAACAGAGGACAAT+GGG 0.446487 4_1:-6222629 Msa0532200:CDS
TTGTCTTCAACACATTCCTT+TGG 0.448621 4_1:-6222098 Msa0532200:CDS
AAGGTCATCCATCAATATAT+AGG 0.449509 4_1:-6221059 Msa0532200:CDS
TGATTTCAGGGCTCATGAAT+GGG 0.451288 4_1:-6221262 Msa0532200:intron
CCTTGAGGACTTGGTCGGAT+TGG 0.454791 4_1:-6221419 Msa0532200:CDS
ATGATTTCAGGGCTCATGAA+TGG 0.456632 4_1:-6221263 Msa0532200:intron
ATACTTAGCATGTTTCTCAA+TGG 0.461652 4_1:+6222140 None:intergenic
GGGTCATTTGGAAACCCTAA+TGG 0.467150 4_1:-6221483 Msa0532200:CDS
TGCATTCATTGGTGTCTCCC+TGG 0.467577 4_1:-6220979 Msa0532200:CDS
TTCCAAGTATCAGGCACACC+AGG 0.469992 4_1:+6220961 None:intergenic
ACCATTGGCAATGGTTTGCA+TGG 0.470878 4_1:+6222558 None:intergenic
TAAGTTCATGTCTGATCAGT+TGG 0.476841 4_1:-6223239 Msa0532200:CDS
TTGAGAAACATGCTAAGTAT+TGG 0.480376 4_1:-6222137 Msa0532200:CDS
TCTTCAACACATTCCTTTGG+TGG 0.480477 4_1:-6222095 Msa0532200:CDS
ACACATTCCTTTGGTGGAGA+AGG 0.487193 4_1:-6222089 Msa0532200:CDS
TCATGAATGGGGAGGAGCAA+AGG 0.487502 4_1:-6221250 Msa0532200:CDS
ATGCCACAACATTGCTTGAA+AGG 0.488238 4_1:-6222684 Msa0532200:CDS
AATAGATCAAATCACAAAAG+AGG 0.493892 4_1:-6221208 Msa0532200:CDS
GTGACTTATTCTATGGTAAG+TGG 0.496743 4_1:-6223306 Msa0532200:CDS
ATCTTAGCTACCAGAATTGG+AGG 0.500091 4_1:-6223192 Msa0532200:CDS
CATGAATGGGGAGGAGCAAA+GGG 0.500571 4_1:-6221249 Msa0532200:CDS
TTTGGCCTTGAAAATGTTGA+GGG 0.501664 4_1:+6222534 None:intergenic
GAAAATGTTGAGGGAACCAT+TGG 0.502111 4_1:+6222543 None:intergenic
CTATCAGAGTACAGAAAAGA+AGG 0.502508 4_1:-6221078 Msa0532200:CDS
CATTGTTGTTCTTTGTAGAG+TGG 0.508085 4_1:+6223262 None:intergenic
TTTGCATGGATTTGAGACTA+AGG 0.509693 4_1:+6222572 None:intergenic
AATGGTTAGAGTCTATGAAA+TGG 0.510756 4_1:-6221442 Msa0532200:CDS
TTGCTTTCCTTCTCCACCAA+AGG 0.511894 4_1:+6222082 None:intergenic
AGACACCAATGAATGCAATC+TGG 0.513904 4_1:+6220985 None:intergenic
CAAGAGGCTTGTGTTTGTTG+GGG 0.515415 4_1:-6222653 Msa0532200:CDS
ATCAATATATAGGAAGCAAT+GGG 0.515450 4_1:-6221049 Msa0532200:CDS
CTTTGGCCTTGAAAATGTTG+AGG 0.517150 4_1:+6222533 None:intergenic
TTGCATGGATTTGAGACTAA+GGG 0.517690 4_1:+6222573 None:intergenic
TTACAAGATTTGAAAACAAG+AGG 0.524741 4_1:-6221132 Msa0532200:CDS
TAAACCATAAGGTGGCAGAT+AGG 0.527182 4_1:-6222179 Msa0532200:CDS
GAGGACTTGGTCGGATTGGT+TGG 0.527840 4_1:-6221415 Msa0532200:CDS
CATCAATATATAGGAAGCAA+TGG 0.529787 4_1:-6221050 Msa0532200:CDS
AGATGATCCAGTAAACCATA+AGG 0.539645 4_1:-6222190 Msa0532200:CDS
AGTAAGGTAAACAAGTATCA+TGG 0.549101 4_1:+6220713 None:intergenic
GTTGAGGGAACCATTGGCAA+TGG 0.550716 4_1:+6222549 None:intergenic
TAGCCTTTCAAGCAATGTTG+TGG 0.553731 4_1:+6222681 None:intergenic
GCCATATATCTAACACAAGA+AGG 0.556284 4_1:-6223379 Msa0532200:CDS
ATAGATCAAATCACAAAAGA+GGG 0.558613 4_1:-6221207 Msa0532200:CDS
CTTGATGTTGACTGAGGTGA+GGG 0.559073 4_1:-6220828 Msa0532200:three_prime_UTR
CAACATTGCTTGAAAGGCTA+AGG 0.559276 4_1:-6222678 Msa0532200:CDS
TGGCTTGTGTGAAGTTTGGA+AGG 0.562230 4_1:-6223219 Msa0532200:CDS
TGACTTATTCTATGGTAAGT+GGG 0.575550 4_1:-6223305 Msa0532200:CDS
GGCTTGTGTGAAGTTTGGAA+GGG 0.576956 4_1:-6223218 Msa0532200:CDS
AATGGCCTTGAGGACTTGGT+CGG 0.580518 4_1:-6221424 Msa0532200:CDS
CCAATCCGACCAAGTCCTCA+AGG 0.581217 4_1:+6221419 None:intergenic
AGAGTTCAAACTACCTTGGT+AGG 0.588819 4_1:+6223149 None:intergenic
TTGAAAGGCTAAGGAACAAG+AGG 0.590198 4_1:-6222669 Msa0532200:CDS
TCTTGATGTTGACTGAGGTG+AGG 0.592402 4_1:-6220829 Msa0532200:three_prime_UTR
CCCTAATGGAGTATACAGAA+AGG 0.595200 4_1:-6221469 Msa0532200:CDS
TTAGCTACCAGAATTGGAGG+TGG 0.598246 4_1:-6223189 Msa0532200:CDS
CCATAAGGTGGCAGATAGGA+CGG 0.601582 4_1:-6222175 Msa0532200:CDS
CTCATCAATGTGACCTACCA+AGG 0.602193 4_1:-6223162 Msa0532200:intron
TGCACATACCTTTGGTGAGT+AGG 0.604829 4_1:+6221342 None:intergenic
CTTGGTAGGTCACATTGATG+AGG 0.614453 4_1:+6223163 None:intergenic
TCCATGCAAACCATTGCCAA+TGG 0.622962 4_1:-6222559 Msa0532200:CDS
TCCTAAAATGATGCAAGTGG+TGG 0.624032 4_1:-6221163 Msa0532200:CDS
GAGTCTATGAAATGGCCTTG+AGG 0.624297 4_1:-6221434 Msa0532200:CDS
TGCATGGATTTGAGACTAAG+GGG 0.624427 4_1:+6222574 None:intergenic
TTGTGTGAAGTTTGGAAGGG+AGG 0.625851 4_1:-6223215 Msa0532200:CDS
GATTTCAGGGCTCATGAATG+GGG 0.627429 4_1:-6221261 Msa0532200:intron
ATTCACTCAACAGAGGACAA+TGG 0.631990 4_1:-6222630 Msa0532200:CDS
CTATGTCACCTACTCACCAA+AGG 0.632176 4_1:-6221350 Msa0532200:intron
ATTTGATTCTACAAGTAATG+GGG 0.634322 4_1:+6222215 None:intergenic
AGTATACAGAAAGGTAGCAA+TGG 0.644531 4_1:-6221460 Msa0532200:CDS
TTCAGGGCTCATGAATGGGG+AGG 0.647228 4_1:-6221258 Msa0532200:intron
TGGGGCCCAATAGAACTCGA+TGG 0.666596 4_1:+6222233 None:intergenic
TAACACAAGAAGGAGGACAA+TGG 0.671835 4_1:-6223369 Msa0532200:CDS
TGATCCAGTAAACCATAAGG+TGG 0.673124 4_1:-6222187 Msa0532200:CDS
TCCAAGTATCAGGCACACCA+GGG 0.675454 4_1:+6220962 None:intergenic
GTTGGGGATTCACTCAACAG+AGG 0.678403 4_1:-6222637 Msa0532200:CDS
TGTTCCTAAAATGATGCAAG+TGG 0.707534 4_1:-6221166 Msa0532200:CDS
GGGGAATTGAAGATTCAACA+AGG 0.716558 4_1:+6222593 None:intergenic
ATATATCTAACACAAGAAGG+AGG 0.717732 4_1:-6223376 Msa0532200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTAAAATTAAAA+AGG + chr4_1:6221323-6221342 None:intergenic 0.0%
!! AAAAAAGTTAAATCATATTA+CGG + chr4_1:6221289-6221308 None:intergenic 10.0%
!!! AGAAAAGTATTTTAAAATTC+AGG - chr4_1:6221214-6221233 Msa0532200:CDS 15.0%
!!! AAATTTTTGAAATGAGAATA+TGG - chr4_1:6221425-6221444 Msa0532200:CDS 15.0%
!!! ACTTTGTTTATGTTAATATA+AGG - chr4_1:6221563-6221582 Msa0532200:intron 15.0%
!! AGCTATAGTTTATTATATTT+AGG + chr4_1:6222958-6222977 None:intergenic 15.0%
!!! TTAGTCATAAAACATTTTGT+AGG + chr4_1:6221978-6221997 None:intergenic 20.0%
!! AATTTGATTCTACAAGTAAT+GGG + chr4_1:6222063-6222082 None:intergenic 20.0%
!!! AAACTCAATCTATAATGTTT+TGG + chr4_1:6222380-6222399 None:intergenic 20.0%
!!! TTGTTGATAGACAATTTTTA+AGG + chr4_1:6222591-6222610 None:intergenic 20.0%
!! TATTACAACACTATCATATA+TGG + chr4_1:6222631-6222650 None:intergenic 20.0%
!! TATTACAACACTATCATATA+TGG + chr4_1:6222631-6222650 None:intergenic 20.0%
!! CATAGAAACAAAAAACAATT+GGG + chr4_1:6222909-6222928 None:intergenic 20.0%
!! ACATAGAAACAAAAAACAAT+TGG + chr4_1:6222910-6222929 None:intergenic 20.0%
! CTTAAAAAAGGATATTGCTA+AGG - chr4_1:6220735-6220754 Msa0532200:three_prime_UTR 25.0%
! TAACAAGAGAAACCAAAAAA+AGG - chr4_1:6220758-6220777 Msa0532200:three_prime_UTR 25.0%
! TCAAAATGTGACTTATTCTA+TGG - chr4_1:6220961-6220980 Msa0532200:CDS 25.0%
!!! TATGGTGTAGTTTGTTTAAT+TGG + chr4_1:6221382-6221401 None:intergenic 25.0%
!! GGAAATCAATTTAGGTTATA+TGG + chr4_1:6221400-6221419 None:intergenic 25.0%
!! CTCATTTCAAAAATTTGCTT+GGG + chr4_1:6221421-6221440 None:intergenic 25.0%
!! TCTCATTTCAAAAATTTGCT+TGG + chr4_1:6221422-6221441 None:intergenic 25.0%
! GAATTGACACATTTAATCAA+AGG - chr4_1:6222006-6222025 Msa0532200:intron 25.0%
! ATTTGATTCTACAAGTAATG+GGG + chr4_1:6222062-6222081 None:intergenic 25.0%
! GAATTTGATTCTACAAGTAA+TGG + chr4_1:6222064-6222083 None:intergenic 25.0%
! ACTAAAAGAGTTGTTGATAT+AGG + chr4_1:6222261-6222280 None:intergenic 25.0%
! ATTGTTCGAAACTTTAATGA+GGG + chr4_1:6222523-6222542 None:intergenic 25.0%
! AATTGTTCGAAACTTTAATG+AGG + chr4_1:6222524-6222543 None:intergenic 25.0%
!!! TTGATAGACAATTTTTAAGG+TGG + chr4_1:6222588-6222607 None:intergenic 25.0%
! GATAGGAATCATTCTATATT+TGG + chr4_1:6222712-6222731 None:intergenic 25.0%
! ACCTAAGATAAAATGATTTC+AGG - chr4_1:6222999-6223018 Msa0532200:intron 25.0%
! CCTAAGATAAAATGATTTCA+GGG - chr4_1:6223000-6223019 Msa0532200:intron 25.0%
! AATAGATCAAATCACAAAAG+AGG - chr4_1:6223066-6223085 Msa0532200:intron 25.0%
! ATAGATCAAATCACAAAAGA+GGG - chr4_1:6223067-6223086 Msa0532200:intron 25.0%
! TTACAAGATTTGAAAACAAG+AGG - chr4_1:6223142-6223161 Msa0532200:intron 25.0%
! ATCAATATATAGGAAGCAAT+GGG - chr4_1:6223225-6223244 Msa0532200:CDS 25.0%
! TTGTCATCTATGGAAAATAT+AGG + chr4_1:6223353-6223372 None:intergenic 25.0%
! TATCAAATGTTTGTCATCTA+TGG + chr4_1:6223363-6223382 None:intergenic 25.0%
! CAATATGTTCCATTTATCTT+TGG - chr4_1:6223399-6223418 Msa0532200:CDS 25.0%
! AAACCAAAACCAAAGATAAA+TGG + chr4_1:6223411-6223430 None:intergenic 25.0%
!!! CATTTGCATTCACCTTTTTT+TGG + chr4_1:6220773-6220792 None:intergenic 30.0%
CAAAAAAAGGTGAATGCAAA+TGG - chr4_1:6220771-6220790 Msa0532200:three_prime_UTR 30.0%
AGTGATTCTGATTCCTAAAT+TGG + chr4_1:6220830-6220849 None:intergenic 30.0%
TCCTTCTTGTGTTAGATATA+TGG + chr4_1:6220899-6220918 None:intergenic 30.0%
ATATATCTAACACAAGAAGG+AGG - chr4_1:6220898-6220917 Msa0532200:three_prime_UTR 30.0%
TGACTTATTCTATGGTAAGT+GGG - chr4_1:6220969-6220988 Msa0532200:CDS 30.0%
ACGCTTAATCACATAGTTAA+TGG + chr4_1:6221177-6221196 None:intergenic 30.0%
!! AAAATTCAGGTTGAATGAGA+CGG - chr4_1:6221227-6221246 Msa0532200:CDS 30.0%
TTTGCTTGGGAAATCAATTT+AGG + chr4_1:6221408-6221427 None:intergenic 30.0%
ATACTTAGCATGTTTCTCAA+TGG + chr4_1:6222137-6222156 None:intergenic 30.0%
TTGAGAAACATGCTAAGTAT+TGG - chr4_1:6222137-6222156 Msa0532200:CDS 30.0%
ACCTGAGACAATAATATGAT+TGG - chr4_1:6222338-6222357 Msa0532200:intron 30.0%
ACCAATCATATTATTGTCTC+AGG + chr4_1:6222342-6222361 None:intergenic 30.0%
ACAACACTATCATATATGGT+GGG + chr4_1:6222627-6222646 None:intergenic 30.0%
TACAACACTATCATATATGG+TGG + chr4_1:6222628-6222647 None:intergenic 30.0%
TGGGTGGAAATATTGATATT+TGG + chr4_1:6222660-6222679 None:intergenic 30.0%
ACAACACTATCATATATGGT+GGG + chr4_1:6222627-6222646 None:intergenic 30.0%
TACAACACTATCATATATGG+TGG + chr4_1:6222628-6222647 None:intergenic 30.0%
! AAAATCAAGTTGCTAACTAG+TGG + chr4_1:6222741-6222760 None:intergenic 30.0%
AATGGTTAGAGTCTATGAAA+TGG - chr4_1:6222832-6222851 Msa0532200:intron 30.0%
! CCCTGAAATCATTTTATCTT+AGG + chr4_1:6223003-6223022 None:intergenic 30.0%
AAGGTCATCCATCAATATAT+AGG - chr4_1:6223215-6223234 Msa0532200:CDS 30.0%
CATTGCTTCCTATATATTGA+TGG + chr4_1:6223226-6223245 None:intergenic 30.0%
CATCAATATATAGGAAGCAA+TGG - chr4_1:6223224-6223243 Msa0532200:CDS 30.0%
! ATGCAATCTGGATAAGATTT+TGG + chr4_1:6223280-6223299 None:intergenic 30.0%
!! GTTCCATTTATCTTTGGTTT+TGG - chr4_1:6223405-6223424 Msa0532200:CDS 30.0%
!!! TGCATGTTTTTTGAGGATTA+GGG + chr4_1:6223499-6223518 None:intergenic 30.0%
!!! ATGCATGTTTTTTGAGGATT+AGG + chr4_1:6223500-6223519 None:intergenic 30.0%
!!! TAGCATATGCATGTTTTTTG+AGG + chr4_1:6223506-6223525 None:intergenic 30.0%
GTGAATGCAAATGGCAATTA+AGG - chr4_1:6220780-6220799 Msa0532200:three_prime_UTR 35.0%
TGGTGTGTAACACTACTATT+TGG + chr4_1:6220810-6220829 None:intergenic 35.0%
GTAGTGTTACACACCAATTT+AGG - chr4_1:6220814-6220833 Msa0532200:three_prime_UTR 35.0%
GCCATATATCTAACACAAGA+AGG - chr4_1:6220895-6220914 Msa0532200:three_prime_UTR 35.0%
GTGACTTATTCTATGGTAAG+TGG - chr4_1:6220968-6220987 Msa0532200:CDS 35.0%
! CATTGTTGTTCTTTGTAGAG+TGG + chr4_1:6221015-6221034 None:intergenic 35.0%
TAAGTTCATGTCTGATCAGT+TGG - chr4_1:6221035-6221054 Msa0532200:CDS 35.0%
ACATAGAGTTCAAACTACCT+TGG + chr4_1:6221132-6221151 None:intergenic 35.0%
! TTGCATGGATTTGAGACTAA+GGG + chr4_1:6221704-6221723 None:intergenic 35.0%
! TTTGCATGGATTTGAGACTA+AGG + chr4_1:6221705-6221724 None:intergenic 35.0%
!! TTTGGCCTTGAAAATGTTGA+GGG + chr4_1:6221743-6221762 None:intergenic 35.0%
AGAGAGGGTGATATAGTTTA+TGG + chr4_1:6221885-6221904 None:intergenic 35.0%
TAGAGACTAAGTAGAAGAGA+GGG + chr4_1:6221900-6221919 None:intergenic 35.0%
TTAGAGACTAAGTAGAAGAG+AGG + chr4_1:6221901-6221920 None:intergenic 35.0%
AGATGATCCAGTAAACCATA+AGG - chr4_1:6222084-6222103 Msa0532200:CDS 35.0%
TTGTCTTCAACACATTCCTT+TGG - chr4_1:6222176-6222195 Msa0532200:CDS 35.0%
ACTTTAATGAGGGTTGACAT+CGG + chr4_1:6222513-6222532 None:intergenic 35.0%
!!! ATTTTTAAGGTGGCATGATG+TGG + chr4_1:6222578-6222597 None:intergenic 35.0%
!! TATATGGTGGGTGGAAATAT+TGG + chr4_1:6222615-6222634 None:intergenic 35.0%
!!! GTGACATTTTAACTACAAGC+AGG - chr4_1:6222765-6222784 Msa0532200:intron 35.0%
!!! ACATTTTAACTACAAGCAGG+TGG - chr4_1:6222768-6222787 Msa0532200:intron 35.0%
!!! CATTTTAACTACAAGCAGGT+GGG - chr4_1:6222769-6222788 Msa0532200:intron 35.0%
!!! ATTTTAACTACAAGCAGGTG+GGG - chr4_1:6222770-6222789 Msa0532200:intron 35.0%
CCTTTCTGTATACTCCATTA+GGG + chr4_1:6222808-6222827 None:intergenic 35.0%
ACCTTTCTGTATACTCCATT+AGG + chr4_1:6222809-6222828 None:intergenic 35.0%
AGTATACAGAAAGGTAGCAA+TGG - chr4_1:6222814-6222833 Msa0532200:intron 35.0%
!! GGGTCTTGTTACGATTAATA+TGG + chr4_1:6222889-6222908 None:intergenic 35.0%
TATTTAGGTGCACATACCTT+TGG + chr4_1:6222943-6222962 None:intergenic 35.0%
ATCACAAAAGAGGGATATAG+TGG - chr4_1:6223076-6223095 Msa0532200:intron 35.0%
! AAAGAGGGATATAGTGGAAA+TGG - chr4_1:6223082-6223101 Msa0532200:intron 35.0%
TGTTCCTAAAATGATGCAAG+TGG - chr4_1:6223108-6223127 Msa0532200:intron 35.0%
CTATCAGAGTACAGAAAAGA+AGG - chr4_1:6223196-6223215 Msa0532200:CDS 35.0%
GATATTTGCTCTTCTGTTAG+AGG + chr4_1:6223253-6223272 None:intergenic 35.0%
CTTATCCAGATTGCATTCAT+TGG - chr4_1:6223284-6223303 Msa0532200:CDS 35.0%
!! TTTTCTCTTGATGTTGACTG+AGG - chr4_1:6223440-6223459 Msa0532200:CDS 35.0%
TAACACAAGAAGGAGGACAA+TGG - chr4_1:6220905-6220924 Msa0532200:three_prime_UTR 40.0%
AGGATCTTAGCTACCAGAAT+TGG - chr4_1:6221079-6221098 Msa0532200:CDS 40.0%
ATCTTAGCTACCAGAATTGG+AGG - chr4_1:6221082-6221101 Msa0532200:CDS 40.0%
AGAGTTCAAACTACCTTGGT+AGG + chr4_1:6221128-6221147 None:intergenic 40.0%
ATGCCACAACATTGCTTGAA+AGG - chr4_1:6221590-6221609 Msa0532200:intron 40.0%
TAGCCTTTCAAGCAATGTTG+TGG + chr4_1:6221596-6221615 None:intergenic 40.0%
CAACATTGCTTGAAAGGCTA+AGG - chr4_1:6221596-6221615 Msa0532200:intron 40.0%
TTGAAAGGCTAAGGAACAAG+AGG - chr4_1:6221605-6221624 Msa0532200:intron 40.0%
AACAAGAGGCTTGTGTTTGT+TGG - chr4_1:6221619-6221638 Msa0532200:intron 40.0%
ACAAGAGGCTTGTGTTTGTT+GGG - chr4_1:6221620-6221639 Msa0532200:intron 40.0%
ATTCACTCAACAGAGGACAA+TGG - chr4_1:6221644-6221663 Msa0532200:intron 40.0%
TTCACTCAACAGAGGACAAT+GGG - chr4_1:6221645-6221664 Msa0532200:intron 40.0%
GGGGAATTGAAGATTCAACA+AGG + chr4_1:6221684-6221703 None:intergenic 40.0%
! TGCATGGATTTGAGACTAAG+GGG + chr4_1:6221703-6221722 None:intergenic 40.0%
GAAAATGTTGAGGGAACCAT+TGG + chr4_1:6221734-6221753 None:intergenic 40.0%
! TGGTTCCCTCAACATTTTCA+AGG - chr4_1:6221735-6221754 Msa0532200:intron 40.0%
!! CTTTGGCCTTGAAAATGTTG+AGG + chr4_1:6221744-6221763 None:intergenic 40.0%
ATGCAACCATCGAGTTCTAT+TGG - chr4_1:6222035-6222054 Msa0532200:intron 40.0%
TGCAACCATCGAGTTCTATT+GGG - chr4_1:6222036-6222055 Msa0532200:intron 40.0%
TGATCCAGTAAACCATAAGG+TGG - chr4_1:6222087-6222106 Msa0532200:CDS 40.0%
TAAACCATAAGGTGGCAGAT+AGG - chr4_1:6222095-6222114 Msa0532200:CDS 40.0%
TCTTCAACACATTCCTTTGG+TGG - chr4_1:6222179-6222198 Msa0532200:CDS 40.0%
! ACACTATCATATATGGTGGG+TGG + chr4_1:6222624-6222643 None:intergenic 40.0%
! ACACTATCATATATGGTGGG+TGG + chr4_1:6222624-6222643 None:intergenic 40.0%
CTAACTAGTGGAGTTAGGAT+AGG + chr4_1:6222729-6222748 None:intergenic 40.0%
AGTTGCTAACTAGTGGAGTT+AGG + chr4_1:6222734-6222753 None:intergenic 40.0%
!! TTTTAACTACAAGCAGGTGG+GGG - chr4_1:6222771-6222790 Msa0532200:intron 40.0%
CCCTAATGGAGTATACAGAA+AGG - chr4_1:6222805-6222824 Msa0532200:intron 40.0%
ATGATTTCAGGGCTCATGAA+TGG - chr4_1:6223011-6223030 Msa0532200:intron 40.0%
TGATTTCAGGGCTCATGAAT+GGG - chr4_1:6223012-6223031 Msa0532200:intron 40.0%
TCCTAAAATGATGCAAGTGG+TGG - chr4_1:6223111-6223130 Msa0532200:intron 40.0%
! TCCACCACTTGCATCATTTT+AGG + chr4_1:6223115-6223134 None:intergenic 40.0%
AGACACCAATGAATGCAATC+TGG + chr4_1:6223292-6223311 None:intergenic 40.0%
AGAAGCTCATTCCAAGTATC+AGG + chr4_1:6223325-6223344 None:intergenic 40.0%
!! CAGTTGGCTTGTGTGAAGTT+TGG - chr4_1:6221051-6221070 Msa0532200:CDS 45.0%
! TGGCTTGTGTGAAGTTTGGA+AGG - chr4_1:6221055-6221074 Msa0532200:CDS 45.0%
GGCTTGTGTGAAGTTTGGAA+GGG - chr4_1:6221056-6221075 Msa0532200:CDS 45.0%
TTGTGTGAAGTTTGGAAGGG+AGG - chr4_1:6221059-6221078 Msa0532200:CDS 45.0%
TTAGCTACCAGAATTGGAGG+TGG - chr4_1:6221085-6221104 Msa0532200:CDS 45.0%
CTTGGTAGGTCACATTGATG+AGG + chr4_1:6221114-6221133 None:intergenic 45.0%
CTCATCAATGTGACCTACCA+AGG - chr4_1:6221112-6221131 Msa0532200:CDS 45.0%
! CAAGAGGCTTGTGTTTGTTG+GGG - chr4_1:6221621-6221640 Msa0532200:intron 45.0%
! CAGAGGACAATGGGTTTCAA+TGG - chr4_1:6221654-6221673 Msa0532200:intron 45.0%
TCCATGCAAACCATTGCCAA+TGG - chr4_1:6221715-6221734 Msa0532200:intron 45.0%
! ACCATTGGCAATGGTTTGCA+TGG + chr4_1:6221719-6221738 None:intergenic 45.0%
!! TTTTCAAGGCCAAAGTGCGT+AGG - chr4_1:6221749-6221768 Msa0532200:intron 45.0%
TCCTATAGACCTACGCACTT+TGG + chr4_1:6221761-6221780 None:intergenic 45.0%
TCTGCCACCTTATGGTTTAC+TGG + chr4_1:6222094-6222113 None:intergenic 45.0%
ACACATTCCTTTGGTGGAGA+AGG - chr4_1:6222185-6222204 Msa0532200:CDS 45.0%
TTGCTTTCCTTCTCCACCAA+AGG + chr4_1:6222195-6222214 None:intergenic 45.0%
GGGTCATTTGGAAACCCTAA+TGG - chr4_1:6222791-6222810 Msa0532200:intron 45.0%
! GAGTCTATGAAATGGCCTTG+AGG - chr4_1:6222840-6222859 Msa0532200:intron 45.0%
! ATGAAATGGCCTTGAGGACT+TGG - chr4_1:6222846-6222865 Msa0532200:intron 45.0%
CTATGTCACCTACTCACCAA+AGG - chr4_1:6222924-6222943 Msa0532200:intron 45.0%
TGCACATACCTTTGGTGAGT+AGG + chr4_1:6222935-6222954 None:intergenic 45.0%
GATTTCAGGGCTCATGAATG+GGG - chr4_1:6223013-6223032 Msa0532200:intron 45.0%
! TCTTGATGTTGACTGAGGTG+AGG - chr4_1:6223445-6223464 Msa0532200:CDS 45.0%
! CTTGATGTTGACTGAGGTGA+GGG - chr4_1:6223446-6223465 Msa0532200:CDS 45.0%
!!! TTTTTTAAAATAATTAAGTT+TGG - chr4_1:6221505-6221524 Msa0532200:intron 5.0%
GTTGGGGATTCACTCAACAG+AGG - chr4_1:6221637-6221656 Msa0532200:intron 50.0%
GTTGAGGGAACCATTGGCAA+TGG + chr4_1:6221728-6221747 None:intergenic 50.0%
GCCAAAGTGCGTAGGTCTAT+AGG - chr4_1:6221757-6221776 Msa0532200:intron 50.0%
CCATAAGGTGGCAGATAGGA+CGG - chr4_1:6222099-6222118 Msa0532200:CDS 50.0%
! AATGGCCTTGAGGACTTGGT+CGG - chr4_1:6222850-6222869 Msa0532200:intron 50.0%
TCATGAATGGGGAGGAGCAA+AGG - chr4_1:6223024-6223043 Msa0532200:intron 50.0%
CATGAATGGGGAGGAGCAAA+GGG - chr4_1:6223025-6223044 Msa0532200:intron 50.0%
! TGCATTCATTGGTGTCTCCC+TGG - chr4_1:6223295-6223314 Msa0532200:CDS 50.0%
TCCAAGTATCAGGCACACCA+GGG + chr4_1:6223315-6223334 None:intergenic 50.0%
TTCCAAGTATCAGGCACACC+AGG + chr4_1:6223316-6223335 None:intergenic 50.0%
GAGGCTTCCACCTCCAATTC+TGG + chr4_1:6221095-6221114 None:intergenic 55.0%
TGGGGCCCAATAGAACTCGA+TGG + chr4_1:6222044-6222063 None:intergenic 55.0%
CCGTCCTATCTGCCACCTTA+TGG + chr4_1:6222102-6222121 None:intergenic 55.0%
ACAAGCAGGTGGGGGTCATT+TGG - chr4_1:6222779-6222798 Msa0532200:intron 55.0%
CCAATCCGACCAAGTCCTCA+AGG + chr4_1:6222858-6222877 None:intergenic 55.0%
! CCTTGAGGACTTGGTCGGAT+TGG - chr4_1:6222855-6222874 Msa0532200:intron 55.0%
!! GAGGACTTGGTCGGATTGGT+TGG - chr4_1:6222859-6222878 Msa0532200:intron 55.0%
TTCAGGGCTCATGAATGGGG+AGG - chr4_1:6223016-6223035 Msa0532200:intron 55.0%
TCCCTGGTGTGCCTGATACT+TGG - chr4_1:6223311-6223330 Msa0532200:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 6220724 6223572 6220724 ID=Msa0532200;Name=Msa0532200
chr4_1 mRNA 6220724 6223572 6220724 ID=Msa0532200-mRNA-1;Parent=Msa0532200;Name=Msa0532200-mRNA-1;_AED=0.30;_eAED=0.30;_QI=60|1|1|1|1|1|5|203|410
chr4_1 exon 6220724 6221275 6220724 ID=Msa0532200-mRNA-1:exon:2243;Parent=Msa0532200-mRNA-1
chr4_1 exon 6221351 6221509 6221351 ID=Msa0532200-mRNA-1:exon:2242;Parent=Msa0532200-mRNA-1
chr4_1 exon 6222072 6222268 6222072 ID=Msa0532200-mRNA-1:exon:2241;Parent=Msa0532200-mRNA-1
chr4_1 exon 6222534 6222711 6222534 ID=Msa0532200-mRNA-1:exon:2240;Parent=Msa0532200-mRNA-1
chr4_1 exon 6223163 6223572 6223163 ID=Msa0532200-mRNA-1:exon:2239;Parent=Msa0532200-mRNA-1
chr4_1 five_prime_UTR 6223513 6223572 6223513 ID=Msa0532200-mRNA-1:five_prime_utr;Parent=Msa0532200-mRNA-1
chr4_1 CDS 6223163 6223512 6223163 ID=Msa0532200-mRNA-1:cds;Parent=Msa0532200-mRNA-1
chr4_1 CDS 6222534 6222711 6222534 ID=Msa0532200-mRNA-1:cds;Parent=Msa0532200-mRNA-1
chr4_1 CDS 6222072 6222268 6222072 ID=Msa0532200-mRNA-1:cds;Parent=Msa0532200-mRNA-1
chr4_1 CDS 6221351 6221509 6221351 ID=Msa0532200-mRNA-1:cds;Parent=Msa0532200-mRNA-1
chr4_1 CDS 6220927 6221275 6220927 ID=Msa0532200-mRNA-1:cds;Parent=Msa0532200-mRNA-1
chr4_1 three_prime_UTR 6220724 6220926 6220724 ID=Msa0532200-mRNA-1:three_prime_utr;Parent=Msa0532200-mRNA-1
Gene Sequence

>Msa0532200

ATGCAAATGGCAATTAAGGAACACCAAATAGTAGTGTTACACACCAATTTAGGAATCAGAATCACTTTTCATTCTCTTGTAGCATTTTTAACAACTATTCTAGTTGTCACTGCCATATATCTAACACAAGAAGGAGGACAATGGTCTCTTGAAAAGACTATCACTTCTGAATCAATGTCAAAATGTGACTTATTCTATGGTAAGTGGGTCTTTGACAATGAATCTTATCCACTCTACAAAGAACAACAATGTAAGTTCATGTCTGATCAGTTGGCTTGTGTGAAGTTTGGAAGGGAGGATCTTAGCTACCAGAATTGGAGGTGGAAGCCTCATCAATGTGACCTACCAAGGTTCAATGCCACAACATTGCTTGAAAGGCTAAGGAACAAGAGGCTTGTGTTTGTTGGGGATTCACTCAACAGAGGACAATGGGTTTCAATGGTTTGCCTTGTTGAATCTTCAATTCCCCTTAGTCTCAAATCCATGCAAACCATTGCCAATGGTTCCCTCAACATTTTCAAGGCCAAAGAATACAATGCAACCATCGAGTTCTATTGGGCCCCATTACTTGTAGAATCAAATTCAGATGATCCAGTAAACCATAAGGTGGCAGATAGGACGGTGAGAGTTCAAGCCATTGAGAAACATGCTAAGTATTGGACTGATGCTGATATTATTGTCTTCAACACATTCCTTTGGTGGAGAAGGAAAGCAATGAATGTTTTGTGGGGGTCATTTGGAAACCCTAATGGAGTATACAGAAAGGTAGCAATGGTTAGAGTCTATGAAATGGCCTTGAGGACTTGGTCGGATTGGTTGGAAGTCCATATTAATCGTAACAAGACCCAATTGTTTTTTGTTTCTATGTCACCTACTCACCAAAGGGCTCATGAATGGGGAGGAGCAAAGGGTGAGAATTGTTACAAAGAAATAGATCAAATCACAAAAGAGGGATATAGTGGAAATGGTTCTGTTCCTAAAATGATGCAAGTGGTGGAAAATGTTTTACAAGATTTGAAAACAAGAGGTTTGAATGTTGAATTGCTTAACATAACACAGCTATCAGAGTACAGAAAAGAAGGTCATCCATCAATATATAGGAAGCAATGGGAGCCTCTAACAGAAGAGCAAATATCAAATCCAAAATCTTATCCAGATTGCATTCATTGGTGTCTCCCTGGTGTGCCTGATACTTGGAATGAGCTTCTTTATGCCTATATTTTCCATAGATGA
Protein sequence

>Msa0532200

MQMAIKEHQIVVLHTNLGIRITFHSLVAFLTTILVVTAIYLTQEGGQWSLEKTITSESMSKCDLFYGKWVFDNESYPLYKEQQCKFMSDQLACVKFGREDLSYQNWRWKPHQCDLPRFNATTLLERLRNKRLVFVGDSLNRGQWVSMVCLVESSIPLSLKSMQTIANGSLNIFKAKEYNATIEFYWAPLLVESNSDDPVNHKVADRTVRVQAIEKHAKYWTDADIIVFNTFLWWRRKAMNVLWGSFGNPNGVYRKVAMVRVYEMALRTWSDWLEVHINRNKTQLFFVSMSPTHQRAHEWGGAKGENCYKEIDQITKEGYSGNGSVPKMMQVVENVLQDLKTRGLNVELLNITQLSEYRKEGHPSIYRKQWEPLTEEQISNPKSYPDCIHWCLPGVPDTWNELLYAYIFHR*