AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0532240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0532240 MtrunA17_Chr4g0005581 91.600 250 3 1 1 232 1 250 3.54e-165 456
Msa0532240 MtrunA17_Chr7g0243241 69.565 253 54 3 1 232 1 251 1.14e-113 325
Msa0532240 MtrunA17_Chr5g0437531 64.516 217 58 2 35 232 28 244 2.50e-88 261
Msa0532240 MtrunA17_Chr5g0431291 70.161 124 33 1 108 231 11 130 5.06e-57 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0532240 AT5G01600.1 61.382 246 65 3 11 232 16 255 3.06e-97 284
Msa0532240 AT2G40300.1 63.839 224 61 2 28 231 34 257 6.82e-93 273
Msa0532240 AT3G56090.1 69.588 194 41 1 56 231 62 255 5.17e-88 261
Msa0532240 AT3G11050.1 62.500 208 60 1 43 232 43 250 6.74e-84 250

Find 74 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGCTCATAATGTTTCTT+TGG 0.198410 4_1:+6264909 Msa0532240:CDS
TGTGGATAACAGGTTCTTTA+AGG 0.220356 4_1:+6265634 Msa0532240:intron
AGCTATCATTGGTGTTTATT+AGG 0.275021 4_1:+6267749 Msa0532240:three_prime_UTR
CATGGAATTGGCTCTGTCTT+TGG 0.276046 4_1:+6266555 Msa0532240:CDS
AGCCTGCTACCTTGCCTGTT+TGG 0.324390 4_1:+6267866 Msa0532240:three_prime_UTR
CAAGCTACAAACAGTTATTA+TGG 0.335464 4_1:-6267638 None:intergenic
AATGCACCAACGGCATTAAC+TGG 0.362157 4_1:+6264835 Msa0532240:CDS
GGCTCTGTCTTTGGAGAAGT+TGG 0.374649 4_1:+6266564 Msa0532240:CDS
AGAATTTGATCATGCTGAAA+AGG 0.375272 4_1:+6266226 Msa0532240:CDS
AAAGAAACATTATGAGCAAT+AGG 0.377860 4_1:-6264907 None:intergenic
TTTGTTCATAATAAACTCTC+TGG 0.379718 4_1:+6267978 Msa0532240:three_prime_UTR
TGAAAAGCTCATGAAATATC+AGG 0.391882 4_1:+6265685 Msa0532240:CDS
TCTGAGGGAGGGCTCACAAT+AGG 0.396058 4_1:-6266206 None:intergenic
TTCTTCTTTGAATCTTCCTA+TGG 0.409275 4_1:+6264768 Msa0532240:CDS
CACGAGTCTAAGCATGTGTT+TGG 0.417495 4_1:-6267817 None:intergenic
CTGAGGGAGGGCTCACAATA+GGG 0.446437 4_1:-6266205 None:intergenic
GAAAATTGAGAAAGGATTGA+AGG 0.451054 4_1:-6264639 None:intergenic
TAGTTTCACTGAGGAATGTT+TGG 0.452988 4_1:+6267711 Msa0532240:three_prime_UTR
ATTAGAGAAGAATAACGAAA+AGG 0.462573 4_1:-6264670 None:intergenic
AATAAATGCACAGCTATCAT+TGG 0.465507 4_1:+6267738 Msa0532240:three_prime_UTR
TCTTTGAATCTTCCTATGGA+TGG 0.467506 4_1:+6264772 Msa0532240:CDS
GTCGTTGAGAAAATTGAGAA+AGG 0.472968 4_1:-6264647 None:intergenic
TTAGAGAAGAATAACGAAAA+GGG 0.474992 4_1:-6264669 None:intergenic
GTGACTCAACTGAGATTAGT+TGG 0.480672 4_1:+6267310 Msa0532240:CDS
TCAAATGTAACAATCCAAAC+AGG 0.482394 4_1:-6267880 None:intergenic
GTGAAGAGGGTGAGGGACAA+AGG 0.485559 4_1:-6264579 None:intergenic
CAGAGACAACGTTGCTCTCA+AGG 0.486549 4_1:+6265504 Msa0532240:intron
TAAATGAAATGTATTCTCCT+AGG 0.493512 4_1:+6267544 Msa0532240:three_prime_UTR
ATCCAAACAGGCAAGGTAGC+AGG 0.495607 4_1:-6267868 None:intergenic
CATAATAAACTCTCTGGGAT+AGG 0.498823 4_1:+6267984 Msa0532240:three_prime_UTR
CCAATTGAGGATCATTGTTA+CGG 0.499037 4_1:-6266747 None:intergenic
TGATTTGTTTAGAACATTCG+TGG 0.499706 4_1:+6266164 Msa0532240:intron
CCGTAACAATGATCCTCAAT+TGG 0.502464 4_1:+6266747 Msa0532240:CDS
TCGATGAAGTCTGCCAATTG+AGG 0.505142 4_1:-6266760 None:intergenic
TTCCTATGGATGGTGATAAG+AGG 0.508563 4_1:+6264782 Msa0532240:CDS
TCATACATCATCTTAATCAA+TGG 0.509628 4_1:-6267476 None:intergenic
AGGTGAAAAGATCGAAACTT+TGG 0.511973 4_1:-6264707 None:intergenic
GTGTGGTTGTGAAGAGGGTG+AGG 0.513478 4_1:-6264587 None:intergenic
TCTTTCTTGACTTCTTCAAA+CGG 0.514880 4_1:-6264871 None:intergenic
GAATTTGATCATGCTGAAAA+GGG 0.518739 4_1:+6266227 Msa0532240:CDS
CAACTGAGATTAGTTGGAAA+GGG 0.526186 4_1:+6267316 Msa0532240:CDS
TTGTTCATAATAAACTCTCT+GGG 0.534044 4_1:+6267979 Msa0532240:three_prime_UTR
GTTAGAAATGAAAATGACGT+TGG 0.536417 4_1:+6267674 Msa0532240:three_prime_UTR
GCGTAAGTTCAAACAAATTG+TGG 0.541977 4_1:+6267771 Msa0532240:three_prime_UTR
TTTCACTGAGGAATGTTTGG+TGG 0.544282 4_1:+6267714 Msa0532240:three_prime_UTR
AGAATTATGTGTAGAAGACT+TGG 0.549163 4_1:+6267613 Msa0532240:three_prime_UTR
GCTTTGTGTGGTTGTGAAGA+GGG 0.551807 4_1:-6264592 None:intergenic
AGGATTGAAGGAAAGAGAGA+TGG 0.560687 4_1:-6264627 None:intergenic
TTCAGCATGATCAAATTCTG+AGG 0.562649 4_1:-6266222 None:intergenic
TCAACTGAGATTAGTTGGAA+AGG 0.563711 4_1:+6267315 Msa0532240:CDS
GGCTTTGTGTGGTTGTGAAG+AGG 0.566471 4_1:-6264593 None:intergenic
TTTGAGAAATGACCCACGAT+AGG 0.571890 4_1:-6264733 None:intergenic
TCAGCATGATCAAATTCTGA+GGG 0.576489 4_1:-6266221 None:intergenic
CAAAGACAGAGCCAATTCCA+TGG 0.587636 4_1:-6266554 None:intergenic
TGCTGCTGCAAATGCACCAA+CGG 0.590613 4_1:+6264825 Msa0532240:CDS
AGAGACAACGTTGCTCTCAA+GGG 0.594095 4_1:+6265505 Msa0532240:intron
GAAAAGGGAGATGCATTGTA+TGG 0.595467 4_1:+6266242 Msa0532240:CDS
TTCCTCTTATCACCATCCAT+AGG 0.596094 4_1:-6264784 None:intergenic
GAACATTCGTGGTGGAAGAG+TGG 0.598139 4_1:+6266175 Msa0532240:intron
AAATGACCCACGATAGGTGA+AGG 0.609219 4_1:-6264727 None:intergenic
AGATTAGTTGGAAAGGGTCA+CGG 0.615348 4_1:+6267322 Msa0532240:CDS
TTAACAATGGCGGCTTTGTG+TGG 0.615407 4_1:-6264604 None:intergenic
GTTCTTTAAGGAATCTAGTG+AGG 0.615734 4_1:+6265646 Msa0532240:CDS
CATGATCAAATTCTGAGGGA+GGG 0.620152 4_1:-6266217 None:intergenic
GCATGATCAAATTCTGAGGG+AGG 0.620192 4_1:-6266218 None:intergenic
ATAACACCAGTTAATGCCGT+TGG 0.628331 4_1:-6264841 None:intergenic
TGTAACAATCCAAACAGGCA+AGG 0.631341 4_1:-6267875 None:intergenic
TGTGGTTGTGAAGAGGGTGA+GGG 0.643337 4_1:-6264586 None:intergenic
AGAGAGATGGTTAACAATGG+CGG 0.662971 4_1:-6264614 None:intergenic
TTCTACGTCTAAAATTACCT+AGG 0.671541 4_1:-6267561 None:intergenic
GAAAGAGAGATGGTTAACAA+TGG 0.681158 4_1:-6264617 None:intergenic
TGATAAGAGGAAGAACGTGA+AGG 0.682141 4_1:+6264795 Msa0532240:CDS
TTTGTTTAGAACATTCGTGG+TGG 0.691243 4_1:+6266167 Msa0532240:intron
ACTACTACGTAGTTTCACTG+AGG 0.744932 4_1:+6267702 Msa0532240:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAATAAAATTCACTTTTT+AGG + chr4_1:6265253-6265272 Msa0532240:intron 10.0%
!!! ATGTATGTAAATTTTTATAA+TGG + chr4_1:6266316-6266335 Msa0532240:intron 10.0%
!!! ATTATTTACTTTTTGCTTTT+TGG + chr4_1:6265052-6265071 Msa0532240:intron 15.0%
!!! AATTTTCAGCTATTTATTTT+AGG + chr4_1:6265153-6265172 Msa0532240:intron 15.0%
!! CATTCATTTAATAATATATG+TGG + chr4_1:6265278-6265297 Msa0532240:intron 15.0%
!!! TAATTTTAATGATGTATGTA+TGG - chr4_1:6265305-6265324 None:intergenic 15.0%
!!! ATTTTGCTTATATTTTGAAA+CGG + chr4_1:6265349-6265368 Msa0532240:intron 15.0%
!! TTAACATAAAATGAATTTAG+AGG - chr4_1:6266081-6266100 None:intergenic 15.0%
!! TGATATAAAATATATGAATG+AGG + chr4_1:6267393-6267412 Msa0532240:intron 15.0%
!! AATGAATGAACCTAAAAAAA+AGG + chr4_1:6265325-6265344 Msa0532240:intron 20.0%
!!! AAGCAAAATTCCTTTTTTTT+AGG - chr4_1:6265338-6265357 None:intergenic 20.0%
!!! ATTGTTAAGTAGTAACTTTT+TGG + chr4_1:6265794-6265813 Msa0532240:intron 20.0%
!!! GTATAATAGCTTGAAATTTT+AGG + chr4_1:6265958-6265977 Msa0532240:intron 20.0%
!! ATTATAATATTCATCATACG+AGG - chr4_1:6266126-6266145 None:intergenic 20.0%
!!! TAATGGTAGTTTTAAGTATA+TGG + chr4_1:6266333-6266352 Msa0532240:intron 20.0%
!! TTAAATATAATCACATGTGT+CGG + chr4_1:6266369-6266388 Msa0532240:intron 20.0%
!! AAAACAACAACAAAAAAAGA+AGG - chr4_1:6266527-6266546 None:intergenic 20.0%
!! TTATGTTTCATGAATATAAC+AGG + chr4_1:6266717-6266736 Msa0532240:intron 20.0%
!! ATGTTTAGGTATAAATATCA+TGG - chr4_1:6266891-6266910 None:intergenic 20.0%
!! ATGAAAAACAAAAACCAATT+CGG + chr4_1:6266909-6266928 Msa0532240:intron 20.0%
!! TAAGAACAAGAAGAAAAAAT+TGG - chr4_1:6267240-6267259 None:intergenic 20.0%
!!! AAGCATTTTTGAAATTGTTT+TGG + chr4_1:6267919-6267938 Msa0532240:three_prime_UTR 20.0%
! TTAGAGAAGAATAACGAAAA+GGG - chr4_1:6264672-6264691 None:intergenic 25.0%
! ATTAGAGAAGAATAACGAAA+AGG - chr4_1:6264673-6264692 None:intergenic 25.0%
!! TTTTCGTTATTCTTCTCTAA+TGG + chr4_1:6264672-6264691 Msa0532240:exon 25.0%
! AAGATTCAAAGAAGAAAAAG+TGG - chr4_1:6264764-6264783 None:intergenic 25.0%
! AAAGAAACATTATGAGCAAT+AGG - chr4_1:6264910-6264929 None:intergenic 25.0%
! TATTGCTCATAATGTTTCTT+TGG + chr4_1:6264909-6264928 Msa0532240:CDS 25.0%
!!! TAGATCTATTTTTTCTCCTT+AGG + chr4_1:6265097-6265116 Msa0532240:intron 25.0%
! AGTAACAACCTATGATAATA+GGG - chr4_1:6265179-6265198 None:intergenic 25.0%
!! GCTTATTTCTTAATACTACA+TGG + chr4_1:6265577-6265596 Msa0532240:intron 25.0%
! ACTTAAAAAGTGAATCATGT+TGG + chr4_1:6265718-6265737 Msa0532240:intron 25.0%
! TTATACAAAAGAATCATGCT+TGG - chr4_1:6265944-6265963 None:intergenic 25.0%
!! TAATAGCTTGAAATTTTAGG+AGG + chr4_1:6265961-6265980 Msa0532240:intron 25.0%
! GAGTCAGCATAAATAATTAA+AGG - chr4_1:6266489-6266508 None:intergenic 25.0%
! ATTAACCATTTGATCTCAAT+AGG - chr4_1:6266686-6266705 None:intergenic 25.0%
! ACTACAAGTATACAGAAAAA+TGG - chr4_1:6266846-6266865 None:intergenic 25.0%
!!! GGTTTTTGTTTTTCATGTTT+AGG - chr4_1:6266905-6266924 None:intergenic 25.0%
! TGAAAAACAAAAACCAATTC+GGG + chr4_1:6266910-6266929 Msa0532240:intron 25.0%
!! TAAGGTTTTTGATATCTCTA+TGG + chr4_1:6266977-6266996 Msa0532240:intron 25.0%
! TAATCTGAAAACATCTTCAA+TGG - chr4_1:6267116-6267135 None:intergenic 25.0%
! TATTACCAAAGTAATGTAAG+TGG - chr4_1:6267362-6267381 None:intergenic 25.0%
! TCATACATCATCTTAATCAA+TGG - chr4_1:6267479-6267498 None:intergenic 25.0%
! TAAATGAAATGTATTCTCCT+AGG + chr4_1:6267544-6267563 Msa0532240:three_prime_UTR 25.0%
! TTTGTTCATAATAAACTCTC+TGG + chr4_1:6267978-6267997 Msa0532240:three_prime_UTR 25.0%
! TTGTTCATAATAAACTCTCT+GGG + chr4_1:6267979-6267998 Msa0532240:three_prime_UTR 25.0%
GAAAATTGAGAAAGGATTGA+AGG - chr4_1:6264642-6264661 None:intergenic 30.0%
TTCTTCTTTGAATCTTCCTA+TGG + chr4_1:6264768-6264787 Msa0532240:CDS 30.0%
TCTTTCTTGACTTCTTCAAA+CGG - chr4_1:6264874-6264893 None:intergenic 30.0%
! ATTTTAGGCCCTATTATCAT+AGG + chr4_1:6265168-6265187 Msa0532240:intron 30.0%
GAGTAACAACCTATGATAAT+AGG - chr4_1:6265180-6265199 None:intergenic 30.0%
!!! TTGCTTATATTTTGAAACGG+AGG + chr4_1:6265352-6265371 Msa0532240:intron 30.0%
!!! TGCTTATATTTTGAAACGGA+GGG + chr4_1:6265353-6265372 Msa0532240:intron 30.0%
ATAATGATGTAAATGCAGTG+TGG + chr4_1:6265435-6265454 Msa0532240:intron 30.0%
TACAAGCTGAATCTTATAGA+GGG - chr4_1:6265557-6265576 None:intergenic 30.0%
! ACTTTGTGAATTTTTGTGTG+TGG + chr4_1:6265616-6265635 Msa0532240:intron 30.0%
! AATTTTTGTGTGTGGATAAC+AGG + chr4_1:6265624-6265643 Msa0532240:intron 30.0%
TGAAAAGCTCATGAAATATC+AGG + chr4_1:6265685-6265704 Msa0532240:CDS 30.0%
!! GTAGTAACTTTTTGGTATAG+TGG + chr4_1:6265802-6265821 Msa0532240:intron 30.0%
!! TGATTTGTTTAGAACATTCG+TGG + chr4_1:6266164-6266183 Msa0532240:intron 30.0%
AGAATTTGATCATGCTGAAA+AGG + chr4_1:6266226-6266245 Msa0532240:CDS 30.0%
GAATTTGATCATGCTGAAAA+GGG + chr4_1:6266227-6266246 Msa0532240:CDS 30.0%
ATCCAAAAATAGAGAGAAAG+AGG - chr4_1:6266625-6266644 None:intergenic 30.0%
TGTTTCCTATTGAGATCAAA+TGG + chr4_1:6266678-6266697 Msa0532240:intron 30.0%
TGTTTCATGAATATAACAGG+TGG + chr4_1:6266720-6266739 Msa0532240:intron 30.0%
!!! CTTTTTTATGACTGTCGTTA+AGG + chr4_1:6266959-6266978 Msa0532240:intron 30.0%
AAACATCTTCAATGGAATCA+AGG - chr4_1:6267108-6267127 None:intergenic 30.0%
! TATATGTATTTTCCTTGCAC+AGG + chr4_1:6267258-6267277 Msa0532240:intron 30.0%
!!! CATTTGTTTTTGTTGCTTGT+AGG + chr4_1:6267426-6267445 Msa0532240:intron 30.0%
!!! ACGTGTTGTTTTTACATTAC+TGG + chr4_1:6267512-6267531 Msa0532240:three_prime_UTR 30.0%
! TTCTACGTCTAAAATTACCT+AGG - chr4_1:6267564-6267583 None:intergenic 30.0%
AGAATTATGTGTAGAAGACT+TGG + chr4_1:6267613-6267632 Msa0532240:three_prime_UTR 30.0%
CAAGCTACAAACAGTTATTA+TGG - chr4_1:6267641-6267660 None:intergenic 30.0%
GTTAGAAATGAAAATGACGT+TGG + chr4_1:6267674-6267693 Msa0532240:three_prime_UTR 30.0%
AATAAATGCACAGCTATCAT+TGG + chr4_1:6267738-6267757 Msa0532240:three_prime_UTR 30.0%
! AGCTATCATTGGTGTTTATT+AGG + chr4_1:6267749-6267768 Msa0532240:three_prime_UTR 30.0%
TCAAATGTAACAATCCAAAC+AGG - chr4_1:6267883-6267902 None:intergenic 30.0%
GAAAGAGAGATGGTTAACAA+TGG - chr4_1:6264620-6264639 None:intergenic 35.0%
GTCGTTGAGAAAATTGAGAA+AGG - chr4_1:6264650-6264669 None:intergenic 35.0%
AGGTGAAAAGATCGAAACTT+TGG - chr4_1:6264710-6264729 None:intergenic 35.0%
TCTTTGAATCTTCCTATGGA+TGG + chr4_1:6264772-6264791 Msa0532240:CDS 35.0%
CTATATTACTGTGCTTCCTA+AGG - chr4_1:6265116-6265135 None:intergenic 35.0%
AAACAAAGAGTGGTACACAT+AGG - chr4_1:6265473-6265492 None:intergenic 35.0%
CAAAGTATGCAAACAAAGAG+TGG - chr4_1:6265483-6265502 None:intergenic 35.0%
!! GGTTTTGTATCATCCTTACT+TGG - chr4_1:6265536-6265555 None:intergenic 35.0%
CTACAAGCTGAATCTTATAG+AGG - chr4_1:6265558-6265577 None:intergenic 35.0%
TGTGGATAACAGGTTCTTTA+AGG + chr4_1:6265634-6265653 Msa0532240:intron 35.0%
! GTTCTTTAAGGAATCTAGTG+AGG + chr4_1:6265646-6265665 Msa0532240:CDS 35.0%
AAGCTCATGAAATATCAGGT+TGG + chr4_1:6265689-6265708 Msa0532240:intron 35.0%
! TTTGTTTAGAACATTCGTGG+TGG + chr4_1:6266167-6266186 Msa0532240:intron 35.0%
TCAGCATGATCAAATTCTGA+GGG - chr4_1:6266224-6266243 None:intergenic 35.0%
TTCAGCATGATCAAATTCTG+AGG - chr4_1:6266225-6266244 None:intergenic 35.0%
!!! TTGTTGTTTTGATTCAGCCA+TGG + chr4_1:6266537-6266556 Msa0532240:intron 35.0%
!! TTTTGATTCAGCCATGGAAT+TGG + chr4_1:6266543-6266562 Msa0532240:intron 35.0%
! TGCCTCTTTCTCTCTATTTT+TGG + chr4_1:6266620-6266639 Msa0532240:intron 35.0%
CCAATTGAGGATCATTGTTA+CGG - chr4_1:6266750-6266769 None:intergenic 35.0%
AGTTTCAACTTGACAGCTTT+AGG - chr4_1:6266818-6266837 None:intergenic 35.0%
! GCAATTCTAAGCTTGGTAAT+TGG - chr4_1:6267065-6267084 None:intergenic 35.0%
TCTCATTGCAATTCTAAGCT+TGG - chr4_1:6267072-6267091 None:intergenic 35.0%
!!! GGAATCAAGGTTTTAAACAG+CGG - chr4_1:6267095-6267114 None:intergenic 35.0%
AGAAAACACCAAGTTACTCT+TGG - chr4_1:6267174-6267193 None:intergenic 35.0%
! TAATTGATTCAACCTGTGCA+AGG - chr4_1:6267273-6267292 None:intergenic 35.0%
! TCAACTGAGATTAGTTGGAA+AGG + chr4_1:6267315-6267334 Msa0532240:CDS 35.0%
! CAACTGAGATTAGTTGGAAA+GGG + chr4_1:6267316-6267335 Msa0532240:CDS 35.0%
TTCCTCCACTTACATTACTT+TGG + chr4_1:6267354-6267373 Msa0532240:intron 35.0%
TACCAAAGTAATGTAAGTGG+AGG - chr4_1:6267359-6267378 None:intergenic 35.0%
TAGTTTCACTGAGGAATGTT+TGG + chr4_1:6267711-6267730 Msa0532240:three_prime_UTR 35.0%
GCGTAAGTTCAAACAAATTG+TGG + chr4_1:6267771-6267790 Msa0532240:three_prime_UTR 35.0%
!! CATAATAAACTCTCTGGGAT+AGG + chr4_1:6267984-6268003 Msa0532240:three_prime_UTR 35.0%
AGAGAGATGGTTAACAATGG+CGG - chr4_1:6264617-6264636 None:intergenic 40.0%
AGGATTGAAGGAAAGAGAGA+TGG - chr4_1:6264630-6264649 None:intergenic 40.0%
! TTTTCACCTTCACCTATCGT+GGG + chr4_1:6264721-6264740 Msa0532240:CDS 40.0%
TTTGAGAAATGACCCACGAT+AGG - chr4_1:6264736-6264755 None:intergenic 40.0%
TTCCTATGGATGGTGATAAG+AGG + chr4_1:6264782-6264801 Msa0532240:CDS 40.0%
TTCCTCTTATCACCATCCAT+AGG - chr4_1:6264787-6264806 None:intergenic 40.0%
TGATAAGAGGAAGAACGTGA+AGG + chr4_1:6264795-6264814 Msa0532240:CDS 40.0%
ATAACACCAGTTAATGCCGT+TGG - chr4_1:6264844-6264863 None:intergenic 40.0%
! CATGATCAAATTCTGAGGGA+GGG - chr4_1:6266220-6266239 None:intergenic 40.0%
GAAAAGGGAGATGCATTGTA+TGG + chr4_1:6266242-6266261 Msa0532240:CDS 40.0%
ACATACATGTGTCAGTGTCT+AGG - chr4_1:6266304-6266323 None:intergenic 40.0%
CCGTAACAATGATCCTCAAT+TGG + chr4_1:6266747-6266766 Msa0532240:CDS 40.0%
! CTTCATCGAAAGCGAGTTTT+TGG + chr4_1:6266774-6266793 Msa0532240:CDS 40.0%
CAAAAATTGCAGTCCCGAAT+TGG - chr4_1:6266926-6266945 None:intergenic 40.0%
! CTTTAGCTCCAAGAGTAACT+TGG + chr4_1:6267163-6267182 Msa0532240:intron 40.0%
GTGACTCAACTGAGATTAGT+TGG + chr4_1:6267310-6267329 Msa0532240:CDS 40.0%
! AGATTAGTTGGAAAGGGTCA+CGG + chr4_1:6267322-6267341 Msa0532240:CDS 40.0%
!! TTTTGTTGCTTGTAGGTGTG+TGG + chr4_1:6267433-6267452 Msa0532240:intron 40.0%
ACTACTACGTAGTTTCACTG+AGG + chr4_1:6267702-6267721 Msa0532240:three_prime_UTR 40.0%
TTTCACTGAGGAATGTTTGG+TGG + chr4_1:6267714-6267733 Msa0532240:three_prime_UTR 40.0%
TGTAACAATCCAAACAGGCA+AGG - chr4_1:6267878-6267897 None:intergenic 40.0%
GCTTTGTGTGGTTGTGAAGA+GGG - chr4_1:6264595-6264614 None:intergenic 45.0%
! TTAACAATGGCGGCTTTGTG+TGG - chr4_1:6264607-6264626 None:intergenic 45.0%
! CTTTTCACCTTCACCTATCG+TGG + chr4_1:6264720-6264739 Msa0532240:CDS 45.0%
AAATGACCCACGATAGGTGA+AGG - chr4_1:6264730-6264749 None:intergenic 45.0%
AATGCACCAACGGCATTAAC+TGG + chr4_1:6264835-6264854 Msa0532240:CDS 45.0%
AGAGACAACGTTGCTCTCAA+GGG + chr4_1:6265505-6265524 Msa0532240:intron 45.0%
! GCATGATCAAATTCTGAGGG+AGG - chr4_1:6266221-6266240 None:intergenic 45.0%
CAAAGACAGAGCCAATTCCA+TGG - chr4_1:6266557-6266576 None:intergenic 45.0%
!! CATGGAATTGGCTCTGTCTT+TGG + chr4_1:6266555-6266574 Msa0532240:CDS 45.0%
TCGATGAAGTCTGCCAATTG+AGG - chr4_1:6266763-6266782 None:intergenic 45.0%
!!! AAGCGAGTTTTTGGTAGAGC+AGG + chr4_1:6266783-6266802 Msa0532240:CDS 45.0%
CACGAGTCTAAGCATGTGTT+TGG - chr4_1:6267820-6267839 None:intergenic 45.0%
TGTGGTTGTGAAGAGGGTGA+GGG - chr4_1:6264589-6264608 None:intergenic 50.0%
! GGCTTTGTGTGGTTGTGAAG+AGG - chr4_1:6264596-6264615 None:intergenic 50.0%
TGCTGCTGCAAATGCACCAA+CGG + chr4_1:6264825-6264844 Msa0532240:CDS 50.0%
CAGAGACAACGTTGCTCTCA+AGG + chr4_1:6265504-6265523 Msa0532240:intron 50.0%
CTCAAGGGACTTGCCAAGTA+AGG + chr4_1:6265520-6265539 Msa0532240:intron 50.0%
! GAACATTCGTGGTGGAAGAG+TGG + chr4_1:6266175-6266194 Msa0532240:intron 50.0%
! GGCTCTGTCTTTGGAGAAGT+TGG + chr4_1:6266564-6266583 Msa0532240:CDS 50.0%
ATCCAAACAGGCAAGGTAGC+AGG - chr4_1:6267871-6267890 None:intergenic 50.0%
GTGAAGAGGGTGAGGGACAA+AGG - chr4_1:6264582-6264601 None:intergenic 55.0%
GTGTGGTTGTGAAGAGGGTG+AGG - chr4_1:6264590-6264609 None:intergenic 55.0%
CTGAGGGAGGGCTCACAATA+GGG - chr4_1:6266208-6266227 None:intergenic 55.0%
TCTGAGGGAGGGCTCACAAT+AGG - chr4_1:6266209-6266228 None:intergenic 55.0%
CTGCAGATCGAGAACGTGCT+TGG - chr4_1:6266420-6266439 None:intergenic 55.0%
AGCCTGCTACCTTGCCTGTT+TGG + chr4_1:6267866-6267885 Msa0532240:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 6264570 6268015 6264570 ID=Msa0532240;Name=Msa0532240
chr4_1 mRNA 6264570 6268015 6264570 ID=Msa0532240-mRNA-1;Parent=Msa0532240;Name=Msa0532240-mRNA-1;_AED=0.15;_eAED=0.15;_QI=121|0.85|0.87|1|1|1|8|533|232
chr4_1 exon 6264570 6264983 6264570 ID=Msa0532240-mRNA-1:exon:2223;Parent=Msa0532240-mRNA-1
chr4_1 exon 6265507 6265536 6265507 ID=Msa0532240-mRNA-1:exon:2224;Parent=Msa0532240-mRNA-1
chr4_1 exon 6265646 6265706 6265646 ID=Msa0532240-mRNA-1:exon:2225;Parent=Msa0532240-mRNA-1
chr4_1 exon 6266176 6266263 6266176 ID=Msa0532240-mRNA-1:exon:2226;Parent=Msa0532240-mRNA-1
chr4_1 exon 6266554 6266615 6266554 ID=Msa0532240-mRNA-1:exon:2227;Parent=Msa0532240-mRNA-1
chr4_1 exon 6266739 6266804 6266739 ID=Msa0532240-mRNA-1:exon:2228;Parent=Msa0532240-mRNA-1
chr4_1 exon 6267280 6267343 6267280 ID=Msa0532240-mRNA-1:exon:2229;Parent=Msa0532240-mRNA-1
chr4_1 exon 6267448 6268015 6267448 ID=Msa0532240-mRNA-1:exon:2230;Parent=Msa0532240-mRNA-1
chr4_1 five_prime_UTR 6264570 6264690 6264570 ID=Msa0532240-mRNA-1:five_prime_utr;Parent=Msa0532240-mRNA-1
chr4_1 CDS 6264691 6264983 6264691 ID=Msa0532240-mRNA-1:cds;Parent=Msa0532240-mRNA-1
chr4_1 CDS 6265507 6265536 6265507 ID=Msa0532240-mRNA-1:cds;Parent=Msa0532240-mRNA-1
chr4_1 CDS 6265646 6265706 6265646 ID=Msa0532240-mRNA-1:cds;Parent=Msa0532240-mRNA-1
chr4_1 CDS 6266176 6266263 6266176 ID=Msa0532240-mRNA-1:cds;Parent=Msa0532240-mRNA-1
chr4_1 CDS 6266554 6266615 6266554 ID=Msa0532240-mRNA-1:cds;Parent=Msa0532240-mRNA-1
chr4_1 CDS 6266739 6266804 6266739 ID=Msa0532240-mRNA-1:cds;Parent=Msa0532240-mRNA-1
chr4_1 CDS 6267280 6267343 6267280 ID=Msa0532240-mRNA-1:cds;Parent=Msa0532240-mRNA-1
chr4_1 CDS 6267448 6267482 6267448 ID=Msa0532240-mRNA-1:cds;Parent=Msa0532240-mRNA-1
chr4_1 three_prime_UTR 6267483 6268015 6267483 ID=Msa0532240-mRNA-1:three_prime_utr;Parent=Msa0532240-mRNA-1
Gene Sequence

>Msa0532240

ATGGCTCTTTCAGCTTCCAAAGTTTCGATCTTTTCACCTTCACCTATCGTGGGTCATTTCTCAAAAAACACCACTTTTTCTTCTTTGAATCTTCCTATGGATGGTGATAAGAGGAAGAACGTGAAGGTTCATGCTGCTGCTGCAAATGCACCAACGGCATTAACTGGTGTTATCTTTGAACCGTTTGAAGAAGTCAAGAAAGATGTTCTTGCTGTTCCTATTGCTCATAATGTTTCTTTGGCTCGTCAGAATTATCAAGATGAAGTTGAATCTGCAATCAATGAACAGATTAAAGACAACGTTGCTCTCAAGGGACTTGCCAAGTTCTTTAAGGAATCTAGTGAGGAAGAAAGAGAGCATGCTGAAAAGCTCATGAAATATCAGAACATTCGTGGTGGAAGAGTGGTGCTGCACCCTATTGTGAGCCCTCCCTCAGAATTTGATCATGCTGAAAAGGGAGATGCATTGTATGCCATGGAATTGGCTCTGTCTTTGGAGAAGTTGGTAAATGAGAAACTTCTGAATGTTCACAGTGTGGCTGACCGTAACAATGATCCTCAATTGGCAGACTTCATCGAAAGCGAGTTTTTGGTAGAGCAGGTTGAATCAATTAAGAAGATATCAGAGTATGTGACTCAACTGAGATTAGTTGGAAAGGGTCACGGTGTGTGGCACTTTGATCAGACTCTTCTCCATTGA
Protein sequence

>Msa0532240

MALSASKVSIFSPSPIVGHFSKNTTFSSLNLPMDGDKRKNVKVHAAAANAPTALTGVIFEPFEEVKKDVLAVPIAHNVSLARQNYQDEVESAINEQIKDNVALKGLAKFFKESSEEEREHAEKLMKYQNIRGGRVVLHPIVSPPSEFDHAEKGDALYAMELALSLEKLVNEKLLNVHSVADRNNDPQLADFIESEFLVEQVESIKKISEYVTQLRLVGKGHGVWHFDQTLLH*