AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0535950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0535950 MtrunA17_Chr4g0001511 93.661 489 23 2 1 489 232 712 0.0 954
Msa0535950 MtrunA17_Chr2g0286741 67.161 472 148 4 16 485 109 575 0.0 657
Msa0535950 MtrunA17_Chr1g0196981 57.495 487 200 3 2 486 5 486 0.0 570
Msa0535950 MtrunA17_Chr8g0388711 54.507 477 209 5 11 485 112 582 0.0 533
Msa0535950 MtrunA17_Chr4g0060941 43.644 472 256 4 19 486 111 576 4.79e-135 402
Msa0535950 MtrunA17_Chr3g0091781 43.958 480 245 9 19 489 73 537 7.24e-133 395
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0535950 AT2G03220.1 47.468 474 235 5 17 484 93 558 6.68e-148 434
Msa0535950 AT2G15370.1 43.515 478 254 6 19 486 61 532 2.89e-138 409
Msa0535950 AT2G03210.3 43.750 496 264 6 1 486 79 569 3.59e-137 407
Msa0535950 AT2G03210.1 43.487 499 264 6 1 486 45 538 5.02e-137 406
Msa0535950 AT2G03210.2 43.487 499 264 6 1 486 79 572 1.02e-136 406
Msa0535950 AT2G15350.1 42.589 479 257 6 19 486 42 513 1.55e-136 404
Msa0535950 AT1G14100.1 43.443 488 244 9 18 488 44 516 2.81e-136 403
Msa0535950 AT2G15390.1 42.589 479 259 7 18 486 30 502 1.65e-135 401
Msa0535950 AT2G15390.2 42.589 479 259 7 18 486 62 534 3.03e-135 401
Msa0535950 AT1G14080.1 43.284 469 250 6 18 476 63 525 2.32e-134 399
Msa0535950 AT1G14110.1 40.890 472 224 6 19 484 46 468 1.74e-123 369
Msa0535950 AT1G74420.2 40.000 455 252 7 10 456 82 523 3.26e-103 318
Msa0535950 AT1G74420.1 39.693 456 254 7 9 456 77 519 4.46e-103 318
Msa0535950 AT1G74420.3 40.000 455 252 7 10 456 120 561 1.32e-102 318
Msa0535950 AT1G74420.4 39.693 456 254 7 9 456 115 557 2.97e-102 317

Find 79 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGTGCAAATACCTTATT+TGG 0.158644 4_1:+12694793 Msa0535950:CDS
TCTAAATTCTAAGAACATTA+AGG 0.197873 4_1:+12694761 Msa0535950:CDS
ACTACTTCTTTGTCTACTTT+TGG 0.201160 4_1:+12695719 Msa0535950:CDS
TTTAAATTATCACCGTATTC+TGG 0.239005 4_1:-12695545 None:intergenic
ACGAATAAATCAACTTCTTC+AGG 0.252310 4_1:+12695578 Msa0535950:CDS
AGCTAACAATAATTCAAAAT+TGG 0.265569 4_1:+12695034 Msa0535950:CDS
CTTGATGGACTCTTAGCTTC+TGG 0.291465 4_1:+12694573 Msa0535950:CDS
TGGACACCGGCTAATGGTTT+AGG 0.295799 4_1:+12694813 Msa0535950:CDS
CATTAGCCGGTGTCCAAATA+AGG 0.310870 4_1:-12694806 None:intergenic
GCTAAAGCAGATGAGAAAAT+AGG 0.324523 4_1:+12695341 Msa0535950:CDS
TTGTGGTAATCCTTACCATT+AGG 0.335624 4_1:-12694723 None:intergenic
CTAAATTCTAAGAACATTAA+GGG 0.340568 4_1:+12694762 Msa0535950:CDS
GTGTGATGCTTGTAAGATTC+AGG 0.349633 4_1:+12694889 Msa0535950:CDS
TAATGTACTTGTCAAATTCA+AGG 0.360755 4_1:+12694285 Msa0535950:five_prime_UTR
TCTTTCATACCAAGTACTTT+TGG 0.362190 4_1:-12695455 None:intergenic
GGATATGTTGCTCAAGGCTT+GGG 0.386546 4_1:+12695740 Msa0535950:CDS
CTTATTTGGACACCGGCTAA+TGG 0.391000 4_1:+12694807 Msa0535950:CDS
CATTTACATGAGGAAATGTA+AGG 0.397294 4_1:+12695913 Msa0535950:CDS
TACAAACCTGTTGTTGTAGC+TGG 0.406944 4_1:-12694352 None:intergenic
TTGCAGCCATGCTAACCATT+TGG 0.409888 4_1:-12694839 None:intergenic
CATAGGTGGTGTGGACCTAA+TGG 0.418999 4_1:+12694708 Msa0535950:CDS
AAATAGTTAGAGATGTTTCA+TGG 0.421838 4_1:+12695970 Msa0535950:exon
GGAAAAGGTAGCGTCCTAAA+TGG 0.422451 4_1:-12695265 None:intergenic
GATTGAACACTCTTATTTGA+AGG 0.425603 4_1:-12695364 None:intergenic
CTCAAGGCTTGGGAAATTTG+AGG 0.429093 4_1:+12695750 Msa0535950:CDS
TTCGATATTAAATATGAAGA+AGG 0.430936 4_1:-12694663 None:intergenic
GAAAGACCAGCTACAACAAC+AGG 0.435452 4_1:+12694346 Msa0535950:five_prime_UTR
TGGATATGTTGCTCAAGGCT+TGG 0.436642 4_1:+12695739 Msa0535950:CDS
CGAATAAATCAACTTCTTCA+GGG 0.450484 4_1:+12695579 Msa0535950:CDS
GCTTCTTGTGGAGTTAAAGT+AGG 0.453450 4_1:-12695386 None:intergenic
TGGTAGTAACTTATTGTGAA+TGG 0.456552 4_1:-12695435 None:intergenic
ATGTAGAGTTGGATCATTTG+TGG 0.462453 4_1:-12694520 None:intergenic
GCGTCTTTATATCCAGAATA+CGG 0.468536 4_1:+12695533 Msa0535950:CDS
TGTTGTAGCTGGTCTTTCTT+CGG 0.470644 4_1:-12694341 None:intergenic
AAATATGAAGAAGGTTTATG+AGG 0.471182 4_1:-12694654 None:intergenic
GCTTGGGAAATTTGAGGCCA+TGG 0.471407 4_1:+12695756 Msa0535950:CDS
TAAGCATAATATGAAAGCTT+GGG 0.479892 4_1:+12695658 Msa0535950:CDS
CTTCATTCGAAGGGTGGAAA+AGG 0.480370 4_1:-12695280 None:intergenic
TTTCCACTACAGTAATGCTT+AGG 0.503251 4_1:-12695863 None:intergenic
ACTAAGTTTATCTGATGTGT+TGG 0.503513 4_1:+12695694 Msa0535950:CDS
CCACTACAGTAATGCTTAGG+AGG 0.503948 4_1:-12695860 None:intergenic
TGGATTGGAGTATGTAGAGT+TGG 0.505395 4_1:-12694531 None:intergenic
TAATGGTTTAGGAAACCAAA+TGG 0.508879 4_1:+12694824 Msa0535950:CDS
ATGATGCCTCATGCGTAAGC+AGG 0.509599 4_1:+12694601 Msa0535950:CDS
AGATTCATAATTGCTTCTTG+TGG 0.518248 4_1:-12695398 None:intergenic
ATCATTTGTGGAACTTGTCA+TGG 0.519995 4_1:-12694508 None:intergenic
ATAAGCATAATATGAAAGCT+TGG 0.522629 4_1:+12695657 Msa0535950:CDS
TCTTTCTTCGGTTGTTGTAG+TGG 0.524722 4_1:-12694329 None:intergenic
TGGTTTCCTAAACCATTAGC+CGG 0.528205 4_1:-12694819 None:intergenic
AAATTTCTATCAAGCACACT+TGG 0.531776 4_1:+12695319 Msa0535950:CDS
AGTCTCCACGCTTCATTCGA+AGG 0.532757 4_1:-12695290 None:intergenic
GAGAACTCTCTTTCACAAGC+TGG 0.535540 4_1:-12695815 None:intergenic
ATTATCTTGTATAGAAGCCA+TGG 0.536564 4_1:-12695773 None:intergenic
ATTTCTCGGGTAAATTTGGT+CGG 0.545196 4_1:-12695091 None:intergenic
AATGTTTGTTGAAAAGGAGA+CGG 0.549584 4_1:+12695235 Msa0535950:CDS
GTCTCCACGCTTCATTCGAA+GGG 0.553860 4_1:-12695289 None:intergenic
CCTCCTAAGCATTACTGTAG+TGG 0.554289 4_1:+12695860 Msa0535950:CDS
CTTCTTCTTTCATTTACATG+AGG 0.557175 4_1:+12695903 Msa0535950:CDS
AATAGTTAGAGATGTTTCAT+GGG 0.557252 4_1:+12695971 Msa0535950:exon
AGGAAACCAAATGGTTAGCA+TGG 0.559741 4_1:+12694833 Msa0535950:CDS
ACTTGTCAAATTCAAGGTGC+CGG 0.569246 4_1:+12694291 Msa0535950:five_prime_UTR
CAAGCATCACACGGTTTGTG+AGG 0.572770 4_1:-12694878 None:intergenic
ATATCAAAGTTTGATAGAGA+AGG 0.577197 4_1:+12695007 Msa0535950:CDS
TTGTAACCTGCTTACGCATG+AGG 0.586351 4_1:-12694607 None:intergenic
TCCACGCTTCATTCGAAGGG+TGG 0.594457 4_1:-12695286 None:intergenic
AAAGGTGTCATGAAAAGAGA+AGG 0.597269 4_1:-12695191 None:intergenic
ATAGAAGTTTATCAAGCAAG+TGG 0.597808 4_1:+12695608 Msa0535950:CDS
TCTGATTGCAAAATTAACAA+AGG 0.600847 4_1:-12695155 None:intergenic
ATCACTAACCAGCTTTACAC+CGG 0.600952 4_1:-12695945 None:intergenic
GTGGTGTGGACCTAATGGTA+AGG 0.605996 4_1:+12694713 Msa0535950:CDS
CAAATACCTTATTTGGACAC+CGG 0.613150 4_1:+12694800 Msa0535950:CDS
AAGTTACTACCAAAAGTACT+TGG 0.622114 4_1:+12695446 Msa0535950:CDS
TTAGGAGGAGCATGATAACA+TGG 0.627761 4_1:-12695845 None:intergenic
TCCACCCTTCGAATGAAGCG+TGG 0.629725 4_1:+12695285 Msa0535950:CDS
AGATTGAGAAATAATGATGA+TGG 0.636757 4_1:-12695062 None:intergenic
TTGTGAAAGAGAGTTCTCAA+TGG 0.648650 4_1:+12695820 Msa0535950:CDS
GAATAAATCAACTTCTTCAG+GGG 0.654370 4_1:+12695580 Msa0535950:CDS
TGAATCTTACAAGCATCACA+CGG 0.663535 4_1:-12694887 None:intergenic
AGATTCAGGAAAGACAAACA+AGG 0.727705 4_1:+12694903 Msa0535950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTCAAAAATAATAAATT+TGG - chr4_1:12694094-12694113 None:intergenic 10.0%
!!! CAATTTGATAATTTTTAAAT+TGG - chr4_1:12694156-12694175 None:intergenic 10.0%
!!! ATGATTAGTATAATAATTTT+TGG + chr4_1:12694441-12694460 Msa0535950:intron 10.0%
!!! TGATTAGTATAATAATTTTT+GGG + chr4_1:12694442-12694461 Msa0535950:intron 10.0%
!! TTTGTCAAAAATAATAAATT+TGG - chr4_1:12694094-12694113 None:intergenic 10.0%
!!! CAATTTGATAATTTTTAAAT+TGG - chr4_1:12694156-12694175 None:intergenic 10.0%
!!! ATGATTAGTATAATAATTTT+TGG + chr4_1:12694441-12694460 Msa0535950:intron 10.0%
!!! TGATTAGTATAATAATTTTT+GGG + chr4_1:12694442-12694461 Msa0535950:intron 10.0%
!!! ACAACTTTTAATTACTCTAT+GGG - chr4_1:12694187-12694206 None:intergenic 20.0%
!!! TACAACTTTTAATTACTCTA+TGG - chr4_1:12694188-12694207 None:intergenic 20.0%
!! TTCGATATTAAATATGAAGA+AGG - chr4_1:12694666-12694685 None:intergenic 20.0%
!!! TCTAAATTCTAAGAACATTA+AGG + chr4_1:12694761-12694780 Msa0535950:CDS 20.0%
!!! CTAAATTCTAAGAACATTAA+GGG + chr4_1:12694762-12694781 Msa0535950:CDS 20.0%
!! AGCTAACAATAATTCAAAAT+TGG + chr4_1:12695034-12695053 Msa0535950:CDS 20.0%
!!! TGAAAAAATGTTTGTTGAAA+AGG + chr4_1:12695229-12695248 Msa0535950:CDS 20.0%
!!! TTATCTTTTAGTTAGTTTCT+TGG + chr4_1:12696002-12696021 Msa0535950:three_prime_UTR 20.0%
!!! TATCTTTTAGTTAGTTTCTT+GGG + chr4_1:12696003-12696022 Msa0535950:three_prime_UTR 20.0%
!!! ACAACTTTTAATTACTCTAT+GGG - chr4_1:12694187-12694206 None:intergenic 20.0%
!!! TACAACTTTTAATTACTCTA+TGG - chr4_1:12694188-12694207 None:intergenic 20.0%
!! TTCGATATTAAATATGAAGA+AGG - chr4_1:12694666-12694685 None:intergenic 20.0%
!!! TCTAAATTCTAAGAACATTA+AGG + chr4_1:12694761-12694780 Msa0535950:CDS 20.0%
!!! CTAAATTCTAAGAACATTAA+GGG + chr4_1:12694762-12694781 Msa0535950:CDS 20.0%
!! AGCTAACAATAATTCAAAAT+TGG + chr4_1:12695034-12695053 Msa0535950:CDS 20.0%
!!! TGAAAAAATGTTTGTTGAAA+AGG + chr4_1:12695229-12695248 Msa0535950:CDS 20.0%
!!! TTATCTTTTAGTTAGTTTCT+TGG + chr4_1:12696002-12696021 Msa0535950:three_prime_UTR 20.0%
!!! TATCTTTTAGTTAGTTTCTT+GGG + chr4_1:12696003-12696022 Msa0535950:three_prime_UTR 20.0%
!!! CAACTTTTAATTACTCTATG+GGG - chr4_1:12694186-12694205 None:intergenic 25.0%
! TAATGTACTTGTCAAATTCA+AGG + chr4_1:12694285-12694304 Msa0535950:five_prime_UTR 25.0%
!! TTTTCTTTGTCATTATGGAT+TGG - chr4_1:12694549-12694568 None:intergenic 25.0%
!! GCAATTTTTCTTTGTCATTA+TGG - chr4_1:12694554-12694573 None:intergenic 25.0%
! AAATATGAAGAAGGTTTATG+AGG - chr4_1:12694657-12694676 None:intergenic 25.0%
! GCAACTATGAAAAAATTCAT+AGG + chr4_1:12694691-12694710 Msa0535950:CDS 25.0%
! ACTATGAAAAAATTCATAGG+TGG + chr4_1:12694694-12694713 Msa0535950:CDS 25.0%
!! AACCAAGTTGAATTCAAAAA+CGG - chr4_1:12694942-12694961 None:intergenic 25.0%
!! ACATGATTTTCATTCCAAAA+AGG - chr4_1:12694981-12695000 None:intergenic 25.0%
! ATATCAAAGTTTGATAGAGA+AGG + chr4_1:12695007-12695026 Msa0535950:CDS 25.0%
! AGATTGAGAAATAATGATGA+TGG - chr4_1:12695065-12695084 None:intergenic 25.0%
! AAAAATTTCTCGGGTAAATT+TGG - chr4_1:12695098-12695117 None:intergenic 25.0%
! ATCACAGTGAAAAAATTTCT+CGG - chr4_1:12695108-12695127 None:intergenic 25.0%
! TCTGATTGCAAAATTAACAA+AGG - chr4_1:12695158-12695177 None:intergenic 25.0%
! TTTAAATTATCACCGTATTC+TGG - chr4_1:12695548-12695567 None:intergenic 25.0%
! ATAAGCATAATATGAAAGCT+TGG + chr4_1:12695657-12695676 Msa0535950:CDS 25.0%
! TAAGCATAATATGAAAGCTT+GGG + chr4_1:12695658-12695677 Msa0535950:CDS 25.0%
!! GAAGAAGCAAAATCTTTTAT+TGG - chr4_1:12695890-12695909 None:intergenic 25.0%
! AAATAGTTAGAGATGTTTCA+TGG + chr4_1:12695970-12695989 Msa0535950:exon 25.0%
! AATAGTTAGAGATGTTTCAT+GGG + chr4_1:12695971-12695990 Msa0535950:exon 25.0%
!!! CAACTTTTAATTACTCTATG+GGG - chr4_1:12694186-12694205 None:intergenic 25.0%
! TAATGTACTTGTCAAATTCA+AGG + chr4_1:12694285-12694304 Msa0535950:five_prime_UTR 25.0%
!! TTTTCTTTGTCATTATGGAT+TGG - chr4_1:12694549-12694568 None:intergenic 25.0%
!! GCAATTTTTCTTTGTCATTA+TGG - chr4_1:12694554-12694573 None:intergenic 25.0%
! AAATATGAAGAAGGTTTATG+AGG - chr4_1:12694657-12694676 None:intergenic 25.0%
! GCAACTATGAAAAAATTCAT+AGG + chr4_1:12694691-12694710 Msa0535950:CDS 25.0%
! ACTATGAAAAAATTCATAGG+TGG + chr4_1:12694694-12694713 Msa0535950:CDS 25.0%
!! AACCAAGTTGAATTCAAAAA+CGG - chr4_1:12694942-12694961 None:intergenic 25.0%
!! ACATGATTTTCATTCCAAAA+AGG - chr4_1:12694981-12695000 None:intergenic 25.0%
! ATATCAAAGTTTGATAGAGA+AGG + chr4_1:12695007-12695026 Msa0535950:CDS 25.0%
! AGATTGAGAAATAATGATGA+TGG - chr4_1:12695065-12695084 None:intergenic 25.0%
! AAAAATTTCTCGGGTAAATT+TGG - chr4_1:12695098-12695117 None:intergenic 25.0%
! ATCACAGTGAAAAAATTTCT+CGG - chr4_1:12695108-12695127 None:intergenic 25.0%
! TCTGATTGCAAAATTAACAA+AGG - chr4_1:12695158-12695177 None:intergenic 25.0%
! TTTAAATTATCACCGTATTC+TGG - chr4_1:12695548-12695567 None:intergenic 25.0%
! ATAAGCATAATATGAAAGCT+TGG + chr4_1:12695657-12695676 Msa0535950:CDS 25.0%
! TAAGCATAATATGAAAGCTT+GGG + chr4_1:12695658-12695677 Msa0535950:CDS 25.0%
!! GAAGAAGCAAAATCTTTTAT+TGG - chr4_1:12695890-12695909 None:intergenic 25.0%
! AAATAGTTAGAGATGTTTCA+TGG + chr4_1:12695970-12695989 Msa0535950:exon 25.0%
! AATAGTTAGAGATGTTTCAT+GGG + chr4_1:12695971-12695990 Msa0535950:exon 25.0%
CGAGCTGAATATAAATATCA+TGG - chr4_1:12694386-12694405 None:intergenic 30.0%
GACAAAGAAAAATTGCTTGA+TGG + chr4_1:12694558-12694577 Msa0535950:CDS 30.0%
! GAATATTTCTCATGCTTTTG+TGG - chr4_1:12694743-12694762 None:intergenic 30.0%
CAAAGTGCAAATACCTTATT+TGG + chr4_1:12694793-12694812 Msa0535950:CDS 30.0%
TAATGGTTTAGGAAACCAAA+TGG + chr4_1:12694824-12694843 Msa0535950:CDS 30.0%
TCACAGTGAAAAAATTTCTC+GGG - chr4_1:12695107-12695126 None:intergenic 30.0%
! AATGTTTGTTGAAAAGGAGA+CGG + chr4_1:12695235-12695254 Msa0535950:CDS 30.0%
AAATTTCTATCAAGCACACT+TGG + chr4_1:12695319-12695338 Msa0535950:CDS 30.0%
! GATTGAACACTCTTATTTGA+AGG - chr4_1:12695367-12695386 None:intergenic 30.0%
AGATTCATAATTGCTTCTTG+TGG - chr4_1:12695401-12695420 None:intergenic 30.0%
TGGTAGTAACTTATTGTGAA+TGG - chr4_1:12695438-12695457 None:intergenic 30.0%
!! AAGTTACTACCAAAAGTACT+TGG + chr4_1:12695446-12695465 Msa0535950:CDS 30.0%
! TCTTTCATACCAAGTACTTT+TGG - chr4_1:12695458-12695477 None:intergenic 30.0%
ACGAATAAATCAACTTCTTC+AGG + chr4_1:12695578-12695597 Msa0535950:CDS 30.0%
CGAATAAATCAACTTCTTCA+GGG + chr4_1:12695579-12695598 Msa0535950:CDS 30.0%
GAATAAATCAACTTCTTCAG+GGG + chr4_1:12695580-12695599 Msa0535950:CDS 30.0%
ATAGAAGTTTATCAAGCAAG+TGG + chr4_1:12695608-12695627 Msa0535950:CDS 30.0%
ACTAAGTTTATCTGATGTGT+TGG + chr4_1:12695694-12695713 Msa0535950:CDS 30.0%
! ACTACTTCTTTGTCTACTTT+TGG + chr4_1:12695719-12695738 Msa0535950:CDS 30.0%
ATTATCTTGTATAGAAGCCA+TGG - chr4_1:12695776-12695795 None:intergenic 30.0%
CTTCTTCTTTCATTTACATG+AGG + chr4_1:12695903-12695922 Msa0535950:CDS 30.0%
CATTTACATGAGGAAATGTA+AGG + chr4_1:12695913-12695932 Msa0535950:CDS 30.0%
! TGAGGAAATGTAAGGATTTT+TGG + chr4_1:12695921-12695940 Msa0535950:CDS 30.0%
!!! AAATGTAAGGATTTTTGGTC+CGG + chr4_1:12695926-12695945 Msa0535950:CDS 30.0%
CGAGCTGAATATAAATATCA+TGG - chr4_1:12694386-12694405 None:intergenic 30.0%
GACAAAGAAAAATTGCTTGA+TGG + chr4_1:12694558-12694577 Msa0535950:CDS 30.0%
! GAATATTTCTCATGCTTTTG+TGG - chr4_1:12694743-12694762 None:intergenic 30.0%
CAAAGTGCAAATACCTTATT+TGG + chr4_1:12694793-12694812 Msa0535950:CDS 30.0%
TAATGGTTTAGGAAACCAAA+TGG + chr4_1:12694824-12694843 Msa0535950:CDS 30.0%
TCACAGTGAAAAAATTTCTC+GGG - chr4_1:12695107-12695126 None:intergenic 30.0%
! AATGTTTGTTGAAAAGGAGA+CGG + chr4_1:12695235-12695254 Msa0535950:CDS 30.0%
AAATTTCTATCAAGCACACT+TGG + chr4_1:12695319-12695338 Msa0535950:CDS 30.0%
! GATTGAACACTCTTATTTGA+AGG - chr4_1:12695367-12695386 None:intergenic 30.0%
AGATTCATAATTGCTTCTTG+TGG - chr4_1:12695401-12695420 None:intergenic 30.0%
TGGTAGTAACTTATTGTGAA+TGG - chr4_1:12695438-12695457 None:intergenic 30.0%
!! AAGTTACTACCAAAAGTACT+TGG + chr4_1:12695446-12695465 Msa0535950:CDS 30.0%
! TCTTTCATACCAAGTACTTT+TGG - chr4_1:12695458-12695477 None:intergenic 30.0%
ACGAATAAATCAACTTCTTC+AGG + chr4_1:12695578-12695597 Msa0535950:CDS 30.0%
CGAATAAATCAACTTCTTCA+GGG + chr4_1:12695579-12695598 Msa0535950:CDS 30.0%
GAATAAATCAACTTCTTCAG+GGG + chr4_1:12695580-12695599 Msa0535950:CDS 30.0%
ATAGAAGTTTATCAAGCAAG+TGG + chr4_1:12695608-12695627 Msa0535950:CDS 30.0%
ACTAAGTTTATCTGATGTGT+TGG + chr4_1:12695694-12695713 Msa0535950:CDS 30.0%
! ACTACTTCTTTGTCTACTTT+TGG + chr4_1:12695719-12695738 Msa0535950:CDS 30.0%
ATTATCTTGTATAGAAGCCA+TGG - chr4_1:12695776-12695795 None:intergenic 30.0%
CTTCTTCTTTCATTTACATG+AGG + chr4_1:12695903-12695922 Msa0535950:CDS 30.0%
CATTTACATGAGGAAATGTA+AGG + chr4_1:12695913-12695932 Msa0535950:CDS 30.0%
! TGAGGAAATGTAAGGATTTT+TGG + chr4_1:12695921-12695940 Msa0535950:CDS 30.0%
!!! AAATGTAAGGATTTTTGGTC+CGG + chr4_1:12695926-12695945 Msa0535950:CDS 30.0%
! GTGGCATTTTGAATTTTCTC+CGG - chr4_1:12694313-12694332 None:intergenic 35.0%
ATCATTTGTGGAACTTGTCA+TGG - chr4_1:12694511-12694530 None:intergenic 35.0%
ATGTAGAGTTGGATCATTTG+TGG - chr4_1:12694523-12694542 None:intergenic 35.0%
GAAAAAATTCATAGGTGGTG+TGG + chr4_1:12694699-12694718 Msa0535950:CDS 35.0%
TTGTGGTAATCCTTACCATT+AGG - chr4_1:12694726-12694745 None:intergenic 35.0%
! CAAATACCTTATTTGGACAC+CGG + chr4_1:12694800-12694819 Msa0535950:CDS 35.0%
TGAATCTTACAAGCATCACA+CGG - chr4_1:12694890-12694909 None:intergenic 35.0%
AGATTCAGGAAAGACAAACA+AGG + chr4_1:12694903-12694922 Msa0535950:CDS 35.0%
!!! AGCCGTTTTTGAATTCAACT+TGG + chr4_1:12694937-12694956 Msa0535950:CDS 35.0%
ATTTCTCGGGTAAATTTGGT+CGG - chr4_1:12695094-12695113 None:intergenic 35.0%
!! AAAGGTGTCATGAAAAGAGA+AGG - chr4_1:12695194-12695213 None:intergenic 35.0%
TCAAGTTCCTTGCTGAAAAA+AGG - chr4_1:12695212-12695231 None:intergenic 35.0%
GCTAAAGCAGATGAGAAAAT+AGG + chr4_1:12695341-12695360 Msa0535950:CDS 35.0%
CAAAAACAAAGCGATTGTGA+AGG + chr4_1:12695499-12695518 Msa0535950:CDS 35.0%
GCGTCTTTATATCCAGAATA+CGG + chr4_1:12695533-12695552 Msa0535950:CDS 35.0%
! ACTTTTGGATATGTTGCTCA+AGG + chr4_1:12695734-12695753 Msa0535950:CDS 35.0%
TTGTGAAAGAGAGTTCTCAA+TGG + chr4_1:12695820-12695839 Msa0535950:CDS 35.0%
TTTCCACTACAGTAATGCTT+AGG - chr4_1:12695866-12695885 None:intergenic 35.0%
! GTGGCATTTTGAATTTTCTC+CGG - chr4_1:12694313-12694332 None:intergenic 35.0%
ATCATTTGTGGAACTTGTCA+TGG - chr4_1:12694511-12694530 None:intergenic 35.0%
ATGTAGAGTTGGATCATTTG+TGG - chr4_1:12694523-12694542 None:intergenic 35.0%
GAAAAAATTCATAGGTGGTG+TGG + chr4_1:12694699-12694718 Msa0535950:CDS 35.0%
TTGTGGTAATCCTTACCATT+AGG - chr4_1:12694726-12694745 None:intergenic 35.0%
! CAAATACCTTATTTGGACAC+CGG + chr4_1:12694800-12694819 Msa0535950:CDS 35.0%
TGAATCTTACAAGCATCACA+CGG - chr4_1:12694890-12694909 None:intergenic 35.0%
AGATTCAGGAAAGACAAACA+AGG + chr4_1:12694903-12694922 Msa0535950:CDS 35.0%
!!! AGCCGTTTTTGAATTCAACT+TGG + chr4_1:12694937-12694956 Msa0535950:CDS 35.0%
ATTTCTCGGGTAAATTTGGT+CGG - chr4_1:12695094-12695113 None:intergenic 35.0%
!! AAAGGTGTCATGAAAAGAGA+AGG - chr4_1:12695194-12695213 None:intergenic 35.0%
TCAAGTTCCTTGCTGAAAAA+AGG - chr4_1:12695212-12695231 None:intergenic 35.0%
GCTAAAGCAGATGAGAAAAT+AGG + chr4_1:12695341-12695360 Msa0535950:CDS 35.0%
CAAAAACAAAGCGATTGTGA+AGG + chr4_1:12695499-12695518 Msa0535950:CDS 35.0%
GCGTCTTTATATCCAGAATA+CGG + chr4_1:12695533-12695552 Msa0535950:CDS 35.0%
! ACTTTTGGATATGTTGCTCA+AGG + chr4_1:12695734-12695753 Msa0535950:CDS 35.0%
TTGTGAAAGAGAGTTCTCAA+TGG + chr4_1:12695820-12695839 Msa0535950:CDS 35.0%
TTTCCACTACAGTAATGCTT+AGG - chr4_1:12695866-12695885 None:intergenic 35.0%
ACTTGTCAAATTCAAGGTGC+CGG + chr4_1:12694291-12694310 Msa0535950:five_prime_UTR 40.0%
TCTTTCTTCGGTTGTTGTAG+TGG - chr4_1:12694332-12694351 None:intergenic 40.0%
!! TGTTGTAGCTGGTCTTTCTT+CGG - chr4_1:12694344-12694363 None:intergenic 40.0%
TACAAACCTGTTGTTGTAGC+TGG - chr4_1:12694355-12694374 None:intergenic 40.0%
TCGATCTCTCTCAAATCTAC+AGG + chr4_1:12694471-12694490 Msa0535950:intron 40.0%
! TGGATTGGAGTATGTAGAGT+TGG - chr4_1:12694534-12694553 None:intergenic 40.0%
TGGTTTCCTAAACCATTAGC+CGG - chr4_1:12694822-12694841 None:intergenic 40.0%
AGGAAACCAAATGGTTAGCA+TGG + chr4_1:12694833-12694852 Msa0535950:CDS 40.0%
GTGTGATGCTTGTAAGATTC+AGG + chr4_1:12694889-12694908 Msa0535950:CDS 40.0%
!!! CATGACACCTTTTTTCAGCA+AGG + chr4_1:12695202-12695221 Msa0535950:CDS 40.0%
! GAGACGGTTTTTCACCATTT+AGG + chr4_1:12695251-12695270 Msa0535950:CDS 40.0%
GCTTCTTGTGGAGTTAAAGT+AGG - chr4_1:12695389-12695408 None:intergenic 40.0%
! TTAGGAGGAGCATGATAACA+TGG - chr4_1:12695848-12695867 None:intergenic 40.0%
ATCACTAACCAGCTTTACAC+CGG - chr4_1:12695948-12695967 None:intergenic 40.0%
ACTTGTCAAATTCAAGGTGC+CGG + chr4_1:12694291-12694310 Msa0535950:five_prime_UTR 40.0%
TCTTTCTTCGGTTGTTGTAG+TGG - chr4_1:12694332-12694351 None:intergenic 40.0%
!! TGTTGTAGCTGGTCTTTCTT+CGG - chr4_1:12694344-12694363 None:intergenic 40.0%
TACAAACCTGTTGTTGTAGC+TGG - chr4_1:12694355-12694374 None:intergenic 40.0%
TCGATCTCTCTCAAATCTAC+AGG + chr4_1:12694471-12694490 Msa0535950:intron 40.0%
! TGGATTGGAGTATGTAGAGT+TGG - chr4_1:12694534-12694553 None:intergenic 40.0%
TGGTTTCCTAAACCATTAGC+CGG - chr4_1:12694822-12694841 None:intergenic 40.0%
AGGAAACCAAATGGTTAGCA+TGG + chr4_1:12694833-12694852 Msa0535950:CDS 40.0%
GTGTGATGCTTGTAAGATTC+AGG + chr4_1:12694889-12694908 Msa0535950:CDS 40.0%
!!! CATGACACCTTTTTTCAGCA+AGG + chr4_1:12695202-12695221 Msa0535950:CDS 40.0%
! GAGACGGTTTTTCACCATTT+AGG + chr4_1:12695251-12695270 Msa0535950:CDS 40.0%
GCTTCTTGTGGAGTTAAAGT+AGG - chr4_1:12695389-12695408 None:intergenic 40.0%
! TTAGGAGGAGCATGATAACA+TGG - chr4_1:12695848-12695867 None:intergenic 40.0%
ATCACTAACCAGCTTTACAC+CGG - chr4_1:12695948-12695967 None:intergenic 40.0%
GAAAGACCAGCTACAACAAC+AGG + chr4_1:12694346-12694365 Msa0535950:five_prime_UTR 45.0%
!! CTTGATGGACTCTTAGCTTC+TGG + chr4_1:12694573-12694592 Msa0535950:CDS 45.0%
TTGTAACCTGCTTACGCATG+AGG - chr4_1:12694610-12694629 None:intergenic 45.0%
CATTAGCCGGTGTCCAAATA+AGG - chr4_1:12694809-12694828 None:intergenic 45.0%
! CTTATTTGGACACCGGCTAA+TGG + chr4_1:12694807-12694826 Msa0535950:CDS 45.0%
TTGCAGCCATGCTAACCATT+TGG - chr4_1:12694842-12694861 None:intergenic 45.0%
! TACCAGAGACGTCTCCTTTT+TGG + chr4_1:12694964-12694983 Msa0535950:CDS 45.0%
TTCCAAAAAGGAGACGTCTC+TGG - chr4_1:12694969-12694988 None:intergenic 45.0%
GGAAAAGGTAGCGTCCTAAA+TGG - chr4_1:12695268-12695287 None:intergenic 45.0%
CTTCATTCGAAGGGTGGAAA+AGG - chr4_1:12695283-12695302 None:intergenic 45.0%
TGGATATGTTGCTCAAGGCT+TGG + chr4_1:12695739-12695758 Msa0535950:CDS 45.0%
GGATATGTTGCTCAAGGCTT+GGG + chr4_1:12695740-12695759 Msa0535950:CDS 45.0%
CTCAAGGCTTGGGAAATTTG+AGG + chr4_1:12695750-12695769 Msa0535950:CDS 45.0%
GAGAACTCTCTTTCACAAGC+TGG - chr4_1:12695818-12695837 None:intergenic 45.0%
CCACTACAGTAATGCTTAGG+AGG - chr4_1:12695863-12695882 None:intergenic 45.0%
CCTCCTAAGCATTACTGTAG+TGG + chr4_1:12695860-12695879 Msa0535950:CDS 45.0%
!! TTTTTGGTCCGGTGTAAAGC+TGG + chr4_1:12695937-12695956 Msa0535950:CDS 45.0%
GAAAGACCAGCTACAACAAC+AGG + chr4_1:12694346-12694365 Msa0535950:five_prime_UTR 45.0%
!! CTTGATGGACTCTTAGCTTC+TGG + chr4_1:12694573-12694592 Msa0535950:CDS 45.0%
TTGTAACCTGCTTACGCATG+AGG - chr4_1:12694610-12694629 None:intergenic 45.0%
CATTAGCCGGTGTCCAAATA+AGG - chr4_1:12694809-12694828 None:intergenic 45.0%
! CTTATTTGGACACCGGCTAA+TGG + chr4_1:12694807-12694826 Msa0535950:CDS 45.0%
TTGCAGCCATGCTAACCATT+TGG - chr4_1:12694842-12694861 None:intergenic 45.0%
! TACCAGAGACGTCTCCTTTT+TGG + chr4_1:12694964-12694983 Msa0535950:CDS 45.0%
TTCCAAAAAGGAGACGTCTC+TGG - chr4_1:12694969-12694988 None:intergenic 45.0%
GGAAAAGGTAGCGTCCTAAA+TGG - chr4_1:12695268-12695287 None:intergenic 45.0%
CTTCATTCGAAGGGTGGAAA+AGG - chr4_1:12695283-12695302 None:intergenic 45.0%
TGGATATGTTGCTCAAGGCT+TGG + chr4_1:12695739-12695758 Msa0535950:CDS 45.0%
GGATATGTTGCTCAAGGCTT+GGG + chr4_1:12695740-12695759 Msa0535950:CDS 45.0%
CTCAAGGCTTGGGAAATTTG+AGG + chr4_1:12695750-12695769 Msa0535950:CDS 45.0%
GAGAACTCTCTTTCACAAGC+TGG - chr4_1:12695818-12695837 None:intergenic 45.0%
CCACTACAGTAATGCTTAGG+AGG - chr4_1:12695863-12695882 None:intergenic 45.0%
CCTCCTAAGCATTACTGTAG+TGG + chr4_1:12695860-12695879 Msa0535950:CDS 45.0%
!! TTTTTGGTCCGGTGTAAAGC+TGG + chr4_1:12695937-12695956 Msa0535950:CDS 45.0%
ATGATGCCTCATGCGTAAGC+AGG + chr4_1:12694601-12694620 Msa0535950:CDS 50.0%
CATAGGTGGTGTGGACCTAA+TGG + chr4_1:12694708-12694727 Msa0535950:CDS 50.0%
GTGGTGTGGACCTAATGGTA+AGG + chr4_1:12694713-12694732 Msa0535950:CDS 50.0%
! TGGACACCGGCTAATGGTTT+AGG + chr4_1:12694813-12694832 Msa0535950:CDS 50.0%
CAAGCATCACACGGTTTGTG+AGG - chr4_1:12694881-12694900 None:intergenic 50.0%
GTCTCCACGCTTCATTCGAA+GGG - chr4_1:12695292-12695311 None:intergenic 50.0%
AGTCTCCACGCTTCATTCGA+AGG - chr4_1:12695293-12695312 None:intergenic 50.0%
! GCTTGGGAAATTTGAGGCCA+TGG + chr4_1:12695756-12695775 Msa0535950:CDS 50.0%
ATGATGCCTCATGCGTAAGC+AGG + chr4_1:12694601-12694620 Msa0535950:CDS 50.0%
CATAGGTGGTGTGGACCTAA+TGG + chr4_1:12694708-12694727 Msa0535950:CDS 50.0%
GTGGTGTGGACCTAATGGTA+AGG + chr4_1:12694713-12694732 Msa0535950:CDS 50.0%
! TGGACACCGGCTAATGGTTT+AGG + chr4_1:12694813-12694832 Msa0535950:CDS 50.0%
CAAGCATCACACGGTTTGTG+AGG - chr4_1:12694881-12694900 None:intergenic 50.0%
GTCTCCACGCTTCATTCGAA+GGG - chr4_1:12695292-12695311 None:intergenic 50.0%
AGTCTCCACGCTTCATTCGA+AGG - chr4_1:12695293-12695312 None:intergenic 50.0%
! GCTTGGGAAATTTGAGGCCA+TGG + chr4_1:12695756-12695775 Msa0535950:CDS 50.0%
TCCACCCTTCGAATGAAGCG+TGG + chr4_1:12695285-12695304 Msa0535950:CDS 55.0%
TCCACGCTTCATTCGAAGGG+TGG - chr4_1:12695289-12695308 None:intergenic 55.0%
TCCACCCTTCGAATGAAGCG+TGG + chr4_1:12695285-12695304 Msa0535950:CDS 55.0%
TCCACGCTTCATTCGAAGGG+TGG - chr4_1:12695289-12695308 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 12694051 12696050 12694051 ID=Msa0535950;Name=Msa0535950
chr4_1 mRNA 12694051 12696050 12694051 ID=Msa0535950-mRNA-1;Parent=Msa0535950;Name=Msa0535950-mRNA-1;_AED=0.19;_eAED=0.19;_QI=334|1|1|1|0|0|2|71|489
chr4_1 exon 12694051 12694367 12694051 ID=Msa0535950-mRNA-1:exon:4029;Parent=Msa0535950-mRNA-1
chr4_1 exon 12694493 12696050 12694493 ID=Msa0535950-mRNA-1:exon:4030;Parent=Msa0535950-mRNA-1
chr4_1 five_prime_UTR 12694051 12694367 12694051 ID=Msa0535950-mRNA-1:five_prime_utr;Parent=Msa0535950-mRNA-1
chr4_1 five_prime_UTR 12694493 12694509 12694493 ID=Msa0535950-mRNA-1:five_prime_utr;Parent=Msa0535950-mRNA-1
chr4_1 CDS 12694510 12695979 12694510 ID=Msa0535950-mRNA-1:cds;Parent=Msa0535950-mRNA-1
chr4_1 three_prime_UTR 12695980 12696050 12695980 ID=Msa0535950-mRNA-1:three_prime_utr;Parent=Msa0535950-mRNA-1
Gene Sequence

>Msa0535950

ATGACAAGTTCCACAAATGATCCAACTCTACATACTCCAATCCATAATGACAAAGAAAAATTGCTTGATGGACTCTTAGCTTCTGGATTTGATGATGCCTCATGCGTAAGCAGGTTACAATCTCATTTATATCGCAAAGTTTCACCTCATAAACCTTCTTCATATTTAATATCGAAACTACGCAACTATGAAAAAATTCATAGGTGGTGTGGACCTAATGGTAAGGATTACCACAAAAGCATGAGAAATATTCTAAATTCTAAGAACATTAAGGGTCATGTTTCAAAGTGCAAATACCTTATTTGGACACCGGCTAATGGTTTAGGAAACCAAATGGTTAGCATGGCTGCAACATTTCTTTATGCTCTCCTCACAAACCGTGTGATGCTTGTAAGATTCAGGAAAGACAAACAAGGTATATTTTGTGAGCCGTTTTTGAATTCAACTTGGTTGTTACCAGAGACGTCTCCTTTTTGGAATGAAAATCATGTTGAAACATATCAAAGTTTGATAGAGAAGGAAAAAGCTAACAATAATTCAAAATTGGAATTGCCATCATCATTATTTCTCAATCTACAATACCGACCAAATTTACCCGAGAAATTTTTTCACTGTGATGATAGTCAAGCTCTTATCATTAAGTTTCCTTTGTTAATTTTGCAATCAGATCAATATTTTGTACCTTCTCTTTTCATGACACCTTTTTTCAGCAAGGAACTTGAAAAAATGTTTGTTGAAAAGGAGACGGTTTTTCACCATTTAGGACGCTACCTTTTCCACCCTTCGAATGAAGCGTGGAGACTAATCACAAATTTCTATCAAGCACACTTGGCTAAAGCAGATGAGAAAATAGGCCTTCAAATAAGAGTGTTCAATCCTACTTTAACTCCACAAGAAGCAATTATGAATCTAGTTTTAAATTGCACCATTCACAATAAGTTACTACCAAAAGTACTTGGTATGAAAGATTCAATGTCTACTTCTAGCAACAAAAACAAAGCGATTGTGAAGGTTGTTCTTGTTGCGTCTTTATATCCAGAATACGGTGATAATTTAAAGACGATGTATACGAATAAATCAACTTCTTCAGGGGAAGTAATAGAAGTTTATCAAGCAAGTGGTGAAGAGCAACAAAAATTTAATGATAATAAGCATAATATGAAAGCTTGGGTTGATATGTATCTACTAAGTTTATCTGATGTGTTGGTTACTACTTCTTTGTCTACTTTTGGATATGTTGCTCAAGGCTTGGGAAATTTGAGGCCATGGCTTCTATACAAGATAATAAGTAATGACACTCATTTTCCAGCTTGTGAAAGAGAGTTCTCAATGGAGCCATGTTATCATGCTCCTCCTAAGCATTACTGTAGTGGAAAGCCAATAAAAGATTTTGCTTCTTCTTTCATTTACATGAGGAAATGTAAGGATTTTTGGTCCGGTGTAAAGCTGGTTAGTGATACAAATAGTTAG
Protein sequence

>Msa0535950

MTSSTNDPTLHTPIHNDKEKLLDGLLASGFDDASCVSRLQSHLYRKVSPHKPSSYLISKLRNYEKIHRWCGPNGKDYHKSMRNILNSKNIKGHVSKCKYLIWTPANGLGNQMVSMAATFLYALLTNRVMLVRFRKDKQGIFCEPFLNSTWLLPETSPFWNENHVETYQSLIEKEKANNNSKLELPSSLFLNLQYRPNLPEKFFHCDDSQALIIKFPLLILQSDQYFVPSLFMTPFFSKELEKMFVEKETVFHHLGRYLFHPSNEAWRLITNFYQAHLAKADEKIGLQIRVFNPTLTPQEAIMNLVLNCTIHNKLLPKVLGMKDSMSTSSNKNKAIVKVVLVASLYPEYGDNLKTMYTNKSTSSGEVIEVYQASGEEQQKFNDNKHNMKAWVDMYLLSLSDVLVTTSLSTFGYVAQGLGNLRPWLLYKIISNDTHFPACEREFSMEPCYHAPPKHYCSGKPIKDFASSFIYMRKCKDFWSGVKLVSDTNS*