Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0539240 | XP_003606493.1 | 92.147 | 191 | 8 | 2 | 19 | 203 | 1 | 190 | 2.55e-125 | 361 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0539240 | sp|Q9SK08|LBD11_ARATH | 66.129 | 186 | 45 | 6 | 32 | 203 | 51 | 232 | 1.21e-74 | 227 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0539240 | G7JRG8 | 92.147 | 191 | 8 | 2 | 19 | 203 | 1 | 190 | 1.22e-125 | 361 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0539240 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0539240 | Msa0602350 | 0.935931 | 3.894386e-97 | -8.615850e-47 |
| Msa0539240 | Msa0643340 | 0.931280 | 4.771651e-94 | -8.615850e-47 |
| Msa0539240 | Msa0683160 | 0.959408 | 2.075608e-117 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0539240 | MtrunA17_Chr4g0030001 | 92.147 | 191 | 8 | 2 | 19 | 203 | 1 | 190 | 2.35e-129 | 361 |
| Msa0539240 | MtrunA17_Chr1g0200201 | 72.024 | 168 | 35 | 6 | 37 | 203 | 73 | 229 | 1.63e-77 | 231 |
| Msa0539240 | MtrunA17_Chr2g0326051 | 69.841 | 126 | 37 | 1 | 78 | 203 | 1 | 125 | 4.15e-61 | 186 |
| Msa0539240 | MtrunA17_Chr2g0311601 | 72.321 | 112 | 30 | 1 | 33 | 144 | 10 | 120 | 2.84e-55 | 172 |
| Msa0539240 | MtrunA17_Chr3g0114061 | 56.000 | 150 | 57 | 3 | 35 | 182 | 7 | 149 | 6.84e-50 | 159 |
| Msa0539240 | MtrunA17_Chr4g0043421 | 66.364 | 110 | 36 | 1 | 35 | 144 | 14 | 122 | 2.65e-49 | 158 |
| Msa0539240 | MtrunA17_Chr5g0437571 | 64.035 | 114 | 40 | 1 | 35 | 148 | 5 | 117 | 4.53e-49 | 157 |
| Msa0539240 | MtrunA17_Chr3g0106101 | 63.636 | 110 | 39 | 1 | 35 | 144 | 6 | 114 | 3.67e-47 | 152 |
| Msa0539240 | MtrunA17_Chr5g0438091 | 56.140 | 114 | 49 | 1 | 35 | 148 | 38 | 150 | 1.41e-41 | 139 |
| Msa0539240 | MtrunA17_Chr3g0129071 | 55.752 | 113 | 49 | 1 | 33 | 145 | 44 | 155 | 4.51e-40 | 136 |
| Msa0539240 | MtrunA17_Chr6g0449641 | 49.180 | 122 | 61 | 1 | 29 | 150 | 2 | 122 | 1.62e-39 | 132 |
| Msa0539240 | MtrunA17_Chr7g0227511 | 54.955 | 111 | 49 | 1 | 28 | 138 | 3 | 112 | 2.48e-39 | 132 |
| Msa0539240 | MtrunA17_Chr3g0117121 | 55.455 | 110 | 48 | 1 | 35 | 144 | 9 | 117 | 2.85e-39 | 133 |
| Msa0539240 | MtrunA17_Chr4g0058651 | 56.481 | 108 | 46 | 1 | 35 | 142 | 10 | 116 | 7.92e-39 | 132 |
| Msa0539240 | MtrunA17_Chr3g0115431 | 56.311 | 103 | 44 | 1 | 35 | 137 | 8 | 109 | 5.48e-38 | 130 |
| Msa0539240 | MtrunA17_Chr6g0449651 | 43.357 | 143 | 67 | 2 | 35 | 177 | 66 | 194 | 1.29e-37 | 129 |
| Msa0539240 | MtrunA17_Chr5g0432451 | 57.522 | 113 | 47 | 1 | 33 | 145 | 40 | 151 | 3.17e-37 | 129 |
| Msa0539240 | MtrunA17_Chr5g0402611 | 53.153 | 111 | 51 | 1 | 35 | 145 | 7 | 116 | 7.03e-36 | 128 |
| Msa0539240 | MtrunA17_Chr7g0225111 | 47.321 | 112 | 58 | 1 | 32 | 143 | 1 | 111 | 1.12e-34 | 119 |
| Msa0539240 | MtrunA17_Chr3g0113891 | 57.732 | 97 | 40 | 1 | 35 | 131 | 13 | 108 | 5.31e-34 | 119 |
| Msa0539240 | MtrunA17_Chr3g0097221 | 50.926 | 108 | 52 | 1 | 35 | 142 | 6 | 112 | 1.50e-33 | 121 |
| Msa0539240 | MtrunA17_Chr5g0437401 | 56.701 | 97 | 41 | 1 | 35 | 131 | 10 | 105 | 3.39e-32 | 114 |
| Msa0539240 | MtrunA17_Chr6g0460951 | 44.118 | 136 | 66 | 4 | 33 | 166 | 37 | 164 | 7.69e-32 | 115 |
| Msa0539240 | MtrunA17_Chr7g0260971 | 52.885 | 104 | 47 | 2 | 35 | 137 | 13 | 115 | 9.25e-32 | 114 |
| Msa0539240 | MtrunA17_Chr8g0353981 | 60.976 | 82 | 31 | 1 | 35 | 116 | 6 | 86 | 1.32e-31 | 114 |
| Msa0539240 | MtrunA17_Chr5g0444351 | 41.964 | 112 | 64 | 1 | 32 | 143 | 1 | 111 | 6.20e-31 | 110 |
| Msa0539240 | MtrunA17_Chr1g0184271 | 40.854 | 164 | 80 | 6 | 36 | 193 | 12 | 164 | 5.25e-30 | 110 |
| Msa0539240 | MtrunA17_Chr1g0184281 | 48.214 | 112 | 55 | 3 | 36 | 145 | 15 | 125 | 1.28e-29 | 108 |
| Msa0539240 | MtrunA17_Chr8g0374601 | 46.667 | 105 | 55 | 1 | 33 | 137 | 23 | 126 | 2.80e-29 | 110 |
| Msa0539240 | MtrunA17_Chr8g0374591 | 41.732 | 127 | 70 | 2 | 33 | 156 | 5 | 130 | 3.73e-29 | 105 |
| Msa0539240 | MtrunA17_Chr7g0245871 | 49.515 | 103 | 50 | 2 | 36 | 137 | 27 | 128 | 5.66e-29 | 108 |
| Msa0539240 | MtrunA17_Chr2g0326041 | 73.611 | 72 | 14 | 2 | 16 | 83 | 9 | 79 | 8.17e-29 | 103 |
| Msa0539240 | MtrunA17_Chr8g0351721 | 49.074 | 108 | 53 | 2 | 35 | 141 | 17 | 123 | 2.37e-28 | 105 |
| Msa0539240 | MtrunA17_Chr8g0351701 | 46.789 | 109 | 56 | 2 | 35 | 142 | 22 | 129 | 3.82e-28 | 105 |
| Msa0539240 | MtrunA17_Chr5g0435641 | 45.378 | 119 | 56 | 3 | 35 | 145 | 6 | 123 | 8.33e-27 | 101 |
| Msa0539240 | MtrunA17_Chr7g0261031 | 52.041 | 98 | 45 | 2 | 35 | 131 | 7 | 103 | 1.38e-26 | 101 |
| Msa0539240 | MtrunA17_Chr8g0374581 | 43.396 | 106 | 60 | 0 | 33 | 138 | 5 | 110 | 3.53e-26 | 100 |
| Msa0539240 | MtrunA17_Chr7g0245861 | 41.525 | 118 | 67 | 1 | 35 | 150 | 11 | 128 | 2.01e-25 | 96.3 |
| Msa0539240 | MtrunA17_Chr6g0452691 | 35.714 | 168 | 89 | 6 | 37 | 199 | 10 | 163 | 4.07e-25 | 96.7 |
| Msa0539240 | MtrunA17_Chr6g0460941 | 44.944 | 89 | 47 | 2 | 36 | 123 | 7 | 94 | 1.41e-23 | 90.1 |
| Msa0539240 | MtrunA17_Chr4g0059901 | 43.617 | 94 | 52 | 1 | 37 | 130 | 11 | 103 | 1.24e-22 | 92.0 |
| Msa0539240 | MtrunA17_Chr6g0452661 | 42.149 | 121 | 58 | 4 | 37 | 149 | 10 | 126 | 1.37e-22 | 91.7 |
| Msa0539240 | MtrunA17_Chr6g0452671 | 48.750 | 80 | 40 | 1 | 37 | 116 | 12 | 90 | 1.18e-20 | 85.5 |
| Msa0539240 | MtrunA17_Chr4g0040831 | 31.210 | 157 | 97 | 4 | 25 | 180 | 4 | 150 | 1.41e-20 | 85.1 |
| Msa0539240 | MtrunA17_Chr4g0055441 | 43.750 | 96 | 48 | 3 | 37 | 127 | 3 | 97 | 1.47e-19 | 81.3 |
| Msa0539240 | MtrunA17_Chr2g0330201 | 37.624 | 101 | 62 | 1 | 37 | 137 | 20 | 119 | 3.65e-19 | 83.2 |
| Msa0539240 | MtrunA17_Chr8g0342351 | 41.489 | 94 | 54 | 1 | 37 | 130 | 13 | 105 | 1.37e-18 | 81.3 |
| Msa0539240 | MtrunA17_Chr5g0439111 | 45.679 | 81 | 42 | 2 | 37 | 116 | 10 | 89 | 8.55e-16 | 73.2 |
| Msa0539240 | MtrunA17_Chr4g0046201 | 34.483 | 87 | 56 | 1 | 37 | 123 | 7 | 92 | 9.86e-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0539240 | AT2G28500.1 | 66.129 | 186 | 45 | 6 | 32 | 203 | 51 | 232 | 1.23e-75 | 227 |
| Msa0539240 | AT1G07900.1 | 62.500 | 176 | 47 | 4 | 32 | 203 | 29 | 189 | 5.14e-72 | 217 |
| Msa0539240 | AT2G30130.1 | 67.890 | 109 | 34 | 1 | 35 | 143 | 7 | 114 | 1.38e-49 | 160 |
| Msa0539240 | AT1G16530.1 | 58.716 | 109 | 45 | 0 | 33 | 141 | 11 | 119 | 1.45e-45 | 149 |
| Msa0539240 | AT1G31320.1 | 68.468 | 111 | 34 | 1 | 35 | 145 | 12 | 121 | 1.05e-43 | 144 |
| Msa0539240 | AT3G27650.1 | 58.654 | 104 | 42 | 1 | 35 | 138 | 38 | 140 | 6.72e-40 | 134 |
| Msa0539240 | AT5G63090.4 | 57.407 | 108 | 45 | 1 | 35 | 142 | 10 | 116 | 7.37e-40 | 135 |
| Msa0539240 | AT5G63090.3 | 57.407 | 108 | 45 | 1 | 35 | 142 | 10 | 116 | 7.37e-40 | 135 |
| Msa0539240 | AT5G63090.2 | 57.407 | 108 | 45 | 1 | 35 | 142 | 10 | 116 | 7.37e-40 | 135 |
| Msa0539240 | AT5G63090.1 | 57.407 | 108 | 45 | 1 | 35 | 142 | 10 | 116 | 7.37e-40 | 135 |
| Msa0539240 | AT2G30340.1 | 50.862 | 116 | 56 | 1 | 33 | 148 | 49 | 163 | 1.55e-39 | 136 |
| Msa0539240 | AT2G30340.2 | 50.862 | 116 | 56 | 1 | 33 | 148 | 50 | 164 | 1.71e-39 | 136 |
| Msa0539240 | AT3G26620.1 | 54.237 | 118 | 49 | 3 | 34 | 147 | 1 | 117 | 1.66e-38 | 129 |
| Msa0539240 | AT2G23660.2 | 55.238 | 105 | 46 | 1 | 35 | 139 | 4 | 107 | 1.85e-38 | 135 |
| Msa0539240 | AT2G23660.3 | 55.238 | 105 | 46 | 1 | 35 | 139 | 4 | 107 | 1.85e-38 | 135 |
| Msa0539240 | AT2G23660.1 | 55.238 | 105 | 46 | 1 | 35 | 139 | 4 | 107 | 1.85e-38 | 135 |
| Msa0539240 | AT3G26660.1 | 56.604 | 106 | 43 | 2 | 34 | 137 | 1 | 105 | 3.51e-38 | 128 |
| Msa0539240 | AT5G66870.1 | 51.128 | 133 | 62 | 2 | 35 | 167 | 6 | 135 | 8.01e-37 | 130 |
| Msa0539240 | AT2G40470.2 | 56.637 | 113 | 48 | 1 | 33 | 145 | 17 | 128 | 4.54e-36 | 125 |
| Msa0539240 | AT2G40470.1 | 56.637 | 113 | 48 | 1 | 33 | 145 | 42 | 153 | 6.60e-36 | 125 |
| Msa0539240 | AT3G11090.1 | 51.923 | 104 | 49 | 1 | 35 | 138 | 10 | 112 | 8.01e-33 | 116 |
| Msa0539240 | AT4G00210.1 | 52.778 | 108 | 49 | 2 | 35 | 141 | 10 | 116 | 1.49e-32 | 117 |
| Msa0539240 | AT1G65620.1 | 60.227 | 88 | 34 | 1 | 29 | 116 | 2 | 88 | 3.16e-32 | 115 |
| Msa0539240 | AT1G65620.4 | 60.227 | 88 | 34 | 1 | 29 | 116 | 2 | 88 | 3.16e-32 | 115 |
| Msa0539240 | AT1G65620.3 | 60.227 | 88 | 34 | 1 | 29 | 116 | 2 | 88 | 3.16e-32 | 115 |
| Msa0539240 | AT1G65620.5 | 60.227 | 88 | 34 | 1 | 29 | 116 | 2 | 88 | 3.16e-32 | 115 |
| Msa0539240 | AT1G65620.2 | 60.227 | 88 | 34 | 1 | 29 | 116 | 2 | 88 | 3.16e-32 | 115 |
| Msa0539240 | AT3G03760.1 | 47.788 | 113 | 57 | 2 | 35 | 146 | 50 | 161 | 3.12e-31 | 115 |
| Msa0539240 | AT2G42430.1 | 46.154 | 117 | 61 | 2 | 31 | 146 | 10 | 125 | 3.00e-29 | 109 |
| Msa0539240 | AT4G00220.1 | 49.524 | 105 | 51 | 2 | 39 | 142 | 20 | 123 | 8.76e-29 | 107 |
| Msa0539240 | AT2G45410.1 | 42.105 | 152 | 69 | 4 | 39 | 189 | 19 | 152 | 1.37e-28 | 106 |
| Msa0539240 | AT2G45420.1 | 47.619 | 105 | 53 | 2 | 39 | 142 | 40 | 143 | 1.09e-26 | 103 |
| Msa0539240 | AT2G42440.1 | 53.608 | 97 | 43 | 2 | 35 | 130 | 6 | 101 | 2.19e-26 | 102 |
| Msa0539240 | AT2G31310.1 | 49.485 | 97 | 47 | 2 | 35 | 130 | 6 | 101 | 2.25e-26 | 100 |
| Msa0539240 | AT3G58190.1 | 38.750 | 160 | 82 | 5 | 36 | 194 | 11 | 155 | 2.81e-26 | 100 |
| Msa0539240 | AT5G06080.1 | 43.363 | 113 | 62 | 2 | 35 | 146 | 6 | 117 | 2.99e-25 | 97.1 |
| Msa0539240 | AT3G47870.1 | 44.860 | 107 | 53 | 2 | 37 | 143 | 37 | 137 | 1.21e-24 | 99.0 |
| Msa0539240 | AT3G50510.1 | 41.441 | 111 | 64 | 1 | 27 | 137 | 3 | 112 | 5.10e-24 | 94.4 |
| Msa0539240 | AT3G50510.2 | 41.441 | 111 | 64 | 1 | 27 | 137 | 3 | 112 | 5.10e-24 | 94.4 |
| Msa0539240 | AT1G72980.1 | 36.232 | 138 | 77 | 3 | 26 | 163 | 5 | 131 | 4.21e-22 | 89.7 |
| Msa0539240 | AT5G35900.1 | 39.604 | 101 | 60 | 1 | 37 | 137 | 6 | 105 | 7.83e-22 | 89.0 |
| Msa0539240 | AT3G13850.1 | 35.256 | 156 | 84 | 5 | 37 | 187 | 37 | 180 | 5.16e-21 | 88.2 |
| Msa0539240 | AT1G06280.1 | 38.824 | 85 | 48 | 2 | 37 | 118 | 25 | 108 | 3.25e-15 | 71.2 |
| Msa0539240 | AT4G22700.1 | 37.864 | 103 | 59 | 4 | 37 | 137 | 6 | 105 | 3.80e-15 | 70.9 |
| Msa0539240 | AT2G19820.1 | 37.634 | 93 | 56 | 2 | 32 | 123 | 8 | 99 | 1.25e-13 | 65.5 |
Find 55 sgRNAs with CRISPR-Local
Find 139 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCAGCTCTTTGAGCTTC+TGG | 0.203584 | 4_1:+19955961 | None:intergenic |
| GAAGCTCATTGACTTGCTTT+TGG | 0.237617 | 4_1:+19955860 | None:intergenic |
| AGAGTTAGAGACCCAGTTTA+TGG | 0.300502 | 4_1:-19955907 | Msa0539240:CDS |
| AAGTGTTTCCTTCACTGATT+TGG | 0.314043 | 4_1:-19955548 | Msa0539240:three_prime_UTR |
| GAGCAACAAGATTGGCTTGC+TGG | 0.325135 | 4_1:+19955791 | None:intergenic |
| GCTCCTGCACAACCATAAAC+TGG | 0.336614 | 4_1:+19955895 | None:intergenic |
| CAACATCATCAAATTCTTAC+AGG | 0.339119 | 4_1:-19957413 | Msa0539240:intron |
| TACAACCTCAGCTTGTGATT+TGG | 0.347506 | 4_1:+19955825 | None:intergenic |
| GTTTGTGTGCACCGGCAATC+TGG | 0.378182 | 4_1:-19955526 | Msa0539240:three_prime_UTR |
| TGCTGCTGATAAATGTGTTT+TGG | 0.380702 | 4_1:-19957500 | Msa0539240:CDS |
| GCATTTGAAGTAATGGAATA+AGG | 0.390026 | 4_1:+19957659 | None:intergenic |
| AATATGATAGCATTACAAAT+AGG | 0.395944 | 4_1:-19955580 | Msa0539240:three_prime_UTR |
| GACCATCTCCGAAGGAGAAA+TGG | 0.396372 | 4_1:-19955332 | Msa0539240:three_prime_UTR |
| CATAGCAATGGACATCATAT+CGG | 0.414964 | 4_1:-19955688 | Msa0539240:CDS |
| GGATCAGTAGGAGGAAAATA+AGG | 0.417860 | 4_1:+19957475 | None:intergenic |
| GCAAAATAGAGCAACAAGAT+TGG | 0.441636 | 4_1:+19955783 | None:intergenic |
| CAGACGATGTGCCAGATTGC+CGG | 0.444100 | 4_1:+19955515 | None:intergenic |
| CGGCAATCTGGCACATCGTC+TGG | 0.445330 | 4_1:-19955514 | Msa0539240:three_prime_UTR |
| CGATATGATGTCCATTGCTA+TGG | 0.449199 | 4_1:+19955689 | None:intergenic |
| GACCCAGTTTATGGTTGTGC+AGG | 0.466543 | 4_1:-19955898 | Msa0539240:CDS |
| TTCCATTTCTCCTTCGGAGA+TGG | 0.469366 | 4_1:+19955330 | None:intergenic |
| TCTATGAGCAATGGTAAACT+TGG | 0.485039 | 4_1:+19957450 | None:intergenic |
| TGTATGTGCATTTGAAGTAA+TGG | 0.489593 | 4_1:+19957652 | None:intergenic |
| AAAAGTAAGGTAGCAGAGCT+AGG | 0.502421 | 4_1:-19955281 | Msa0539240:three_prime_UTR |
| ATATATAACTAATAAAAGTA+AGG | 0.506193 | 4_1:-19955294 | Msa0539240:three_prime_UTR |
| AGTGACAAGCATGGTTTATG+AGG | 0.515235 | 4_1:-19955938 | Msa0539240:CDS |
| TGAGCAATGGTAAACTTGGC+AGG | 0.527769 | 4_1:+19957454 | None:intergenic |
| GCTCAGGGTGGGATACACTT+TGG | 0.537894 | 4_1:-19955633 | Msa0539240:CDS |
| AGTGTTATTTATTAGTAACA+TGG | 0.537934 | 4_1:-19957605 | Msa0539240:CDS |
| CTGATTTGGTTTGTGTGCAC+CGG | 0.542828 | 4_1:-19955534 | Msa0539240:three_prime_UTR |
| GACAACACTAGCCTATGCTC+AGG | 0.542861 | 4_1:-19955649 | Msa0539240:CDS |
| CTCCTGCACAACCATAAACT+GGG | 0.571360 | 4_1:+19955896 | None:intergenic |
| GTAAACTTGGCAGGATCAGT+AGG | 0.577739 | 4_1:+19957463 | None:intergenic |
| AAAATCTTGCAAGCAGCACA+AGG | 0.584368 | 4_1:+19957535 | None:intergenic |
| TGATGTCCATTGCTATGGGA+AGG | 0.587814 | 4_1:+19955694 | None:intergenic |
| AACTGTTGTGGTGATTCCTG+TGG | 0.589765 | 4_1:+19955742 | None:intergenic |
| GTGTATCCCACCCTGAGCAT+AGG | 0.591383 | 4_1:+19955638 | None:intergenic |
| GATATGATGTCCATTGCTAT+GGG | 0.597738 | 4_1:+19955690 | None:intergenic |
| CACTAGCCTATGCTCAGGGT+GGG | 0.599833 | 4_1:-19955644 | Msa0539240:CDS |
| ATCGTCTGGTAAAGATTAAG+CGG | 0.601837 | 4_1:-19955500 | Msa0539240:three_prime_UTR |
| GGAAATGTCACAAAGTCCAC+AGG | 0.603176 | 4_1:-19955758 | Msa0539240:CDS |
| AGCTAGGATTAATTTCCTGT+AGG | 0.610831 | 4_1:-19955265 | Msa0539240:three_prime_UTR |
| CAAAGTCCTTCCCATAGCAA+TGG | 0.614127 | 4_1:-19955700 | Msa0539240:CDS |
| AGCTGATGCAGTGACAAGCA+TGG | 0.615883 | 4_1:-19955947 | Msa0539240:CDS |
| AATTTGATGACCATCTCCGA+AGG | 0.635642 | 4_1:-19955340 | Msa0539240:three_prime_UTR |
| CACACAAACCAAATCAGTGA+AGG | 0.638489 | 4_1:+19955540 | None:intergenic |
| AACTTGGCAGGATCAGTAGG+AGG | 0.640755 | 4_1:+19957466 | None:intergenic |
| ATACACTTTGGACATGAACA+AGG | 0.645234 | 4_1:-19955621 | Msa0539240:exon |
| ATCACCACAACAGTTATCAG+TGG | 0.656563 | 4_1:-19955734 | Msa0539240:CDS |
| ACTAGCCAAATCACAAGCTG+AGG | 0.662044 | 4_1:-19955830 | Msa0539240:CDS |
| CACGCAGTCAGTCTCATCGA+TGG | 0.666062 | 4_1:+19955394 | None:intergenic |
| ACAACACTAGCCTATGCTCA+GGG | 0.673423 | 4_1:-19955648 | Msa0539240:CDS |
| TCATCCACTGATAACTGTTG+TGG | 0.694554 | 4_1:+19955730 | None:intergenic |
| ACACTAGCCTATGCTCAGGG+TGG | 0.697314 | 4_1:-19955645 | Msa0539240:CDS |
| TTTGGACATGAACAAGGACA+AGG | 0.723771 | 4_1:-19955615 | Msa0539240:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATAACTAATAAAAGTA+AGG | - | chr4_1:19957536-19957555 | Msa0539240:CDS | 10.0% |
| !! | AAACATATAGAAAAAAACAA+AGG | - | chr4_1:19955200-19955219 | Msa0539240:three_prime_UTR | 15.0% |
| !! | AACATATAGAAAAAAACAAA+GGG | - | chr4_1:19955201-19955220 | Msa0539240:three_prime_UTR | 15.0% |
| !!! | CAATTTTAATATTGATTTGT+AGG | - | chr4_1:19955759-19955778 | Msa0539240:CDS | 15.0% |
| !! | AAGATGGAATAATAATATAT+AGG | - | chr4_1:19955827-19955846 | Msa0539240:CDS | 15.0% |
| !! | GAAACAAAACAAAAAAATTT+AGG | - | chr4_1:19956102-19956121 | Msa0539240:intron | 15.0% |
| !!! | TACTATTTGTTAGAATTATT+AGG | + | chr4_1:19956276-19956295 | None:intergenic | 15.0% |
| !!! | TTTTTGTTGTTGAAAAAAAT+AGG | - | chr4_1:19956841-19956860 | Msa0539240:intron | 15.0% |
| !!! | AGTGTTATTTATTAGTAACA+TGG | - | chr4_1:19955225-19955244 | Msa0539240:three_prime_UTR | 20.0% |
| !! | ACTAGAAAATGTTGAAATAT+TGG | - | chr4_1:19955451-19955470 | Msa0539240:three_prime_UTR | 20.0% |
| !! | TATACATTCAACATTATCAA+AGG | - | chr4_1:19956020-19956039 | Msa0539240:intron | 20.0% |
| !!! | ATTTATTTGAGGCATTATAA+CGG | + | chr4_1:19956240-19956259 | None:intergenic | 20.0% |
| !!! | TATTTGTTAGAATTATTAGG+TGG | + | chr4_1:19956273-19956292 | None:intergenic | 20.0% |
| !!! | ATTCTTTAGCTTCTATTTTT+AGG | + | chr4_1:19956527-19956546 | None:intergenic | 20.0% |
| !! | AAAAATAGAAGCTAAAGAAT+AGG | - | chr4_1:19956527-19956546 | Msa0539240:intron | 20.0% |
| !!! | ATTTTTCTTGAAAAACCTAA+AGG | - | chr4_1:19956740-19956759 | Msa0539240:intron | 20.0% |
| !!! | TTTTTTTTCCTTAAACCTTT+AGG | + | chr4_1:19956758-19956777 | None:intergenic | 20.0% |
| !! | AATATGATAGCATTACAAAT+AGG | - | chr4_1:19957250-19957269 | Msa0539240:intron | 20.0% |
| !!! | ATTTTTCTTTCAAGGAAAAA+TGG | - | chr4_1:19957590-19957609 | Msa0539240:CDS | 20.0% |
| !! | GATGTACTATTAAAAAAAGT+TGG | - | chr4_1:19957612-19957631 | Msa0539240:CDS | 20.0% |
| ! | TTTGAATCAATGATGAAAGA+TGG | - | chr4_1:19955811-19955830 | Msa0539240:CDS | 25.0% |
| !!! | GTATCAAGATTTACTTTTTG+AGG | - | chr4_1:19955986-19956005 | Msa0539240:intron | 25.0% |
| ! | TTCTAACAAATAGTACCTAA+TGG | - | chr4_1:19956281-19956300 | Msa0539240:intron | 25.0% |
| ! | TCTAACAAATAGTACCTAAT+GGG | - | chr4_1:19956282-19956301 | Msa0539240:intron | 25.0% |
| !!! | TCTATTTTTAGGTCAAGATT+TGG | + | chr4_1:19956516-19956535 | None:intergenic | 25.0% |
| ! | TTGAAAAACCTAAAGGTTTA+AGG | - | chr4_1:19956747-19956766 | Msa0539240:intron | 25.0% |
| ! | TTGAAAGAAAAATAACCTAC+AGG | + | chr4_1:19957583-19957602 | None:intergenic | 25.0% |
| !!! | TGTAGGTTATTTTTCTTTCA+AGG | - | chr4_1:19957582-19957601 | Msa0539240:CDS | 25.0% |
| ! | TGTATGTGCATTTGAAGTAA+TGG | + | chr4_1:19955181-19955200 | None:intergenic | 30.0% |
| CAACATCATCAAATTCTTAC+AGG | - | chr4_1:19955417-19955436 | Msa0539240:three_prime_UTR | 30.0% | |
| ATTGTTGTGATCTAAGTAAG+TGG | - | chr4_1:19955909-19955928 | Msa0539240:CDS | 30.0% | |
| TTTAGGAGTGAAACATGATA+AGG | - | chr4_1:19956119-19956138 | Msa0539240:intron | 30.0% | |
| CCATGGACCATAATAAAAAA+GGG | + | chr4_1:19956197-19956216 | None:intergenic | 30.0% | |
| TCCATGGACCATAATAAAAA+AGG | + | chr4_1:19956198-19956217 | None:intergenic | 30.0% | |
| TTTATTATGGTCCATGGATA+TGG | - | chr4_1:19956200-19956219 | Msa0539240:intron | 30.0% | |
| GTTGACAATGCATTTATTTG+AGG | + | chr4_1:19956251-19956270 | None:intergenic | 30.0% | |
| CTAACAAATAGTACCTAATG+GGG | - | chr4_1:19956283-19956302 | Msa0539240:intron | 30.0% | |
| TAACAAATAGTACCTAATGG+GGG | - | chr4_1:19956284-19956303 | Msa0539240:intron | 30.0% | |
| TTTGTATGACCCAGATTTAA+TGG | + | chr4_1:19956340-19956359 | None:intergenic | 30.0% | |
| AAGTGTTATGTATGACTACA+AGG | + | chr4_1:19956486-19956505 | None:intergenic | 30.0% | |
| AATAGAAGCTAAAGAATAGG+AGG | - | chr4_1:19956530-19956549 | Msa0539240:intron | 30.0% | |
| TAGTCATGTATTTCAGAGTT+CGG | + | chr4_1:19956720-19956739 | None:intergenic | 30.0% | |
| ! | TGATTTTTCCATTTCTCCTT+CGG | + | chr4_1:19957509-19957528 | None:intergenic | 30.0% |
| ! | TTTTCATCTCGAATTGATGT+GGG | + | chr4_1:19957642-19957661 | None:intergenic | 30.0% |
| ! | TTTTTCATCTCGAATTGATG+TGG | + | chr4_1:19957643-19957662 | None:intergenic | 30.0% |
| ! | TGCTGCTGATAAATGTGTTT+TGG | - | chr4_1:19955330-19955349 | Msa0539240:three_prime_UTR | 35.0% |
| TCTATGAGCAATGGTAAACT+TGG | + | chr4_1:19955383-19955402 | None:intergenic | 35.0% | |
| ! | ACCATTGCTCATAGAGTTTT+TGG | - | chr4_1:19955388-19955407 | Msa0539240:three_prime_UTR | 35.0% |
| ACCAAAAACTCTATGAGCAA+TGG | + | chr4_1:19955392-19955411 | None:intergenic | 35.0% | |
| TGATCTAAGTAAGTGGTAAG+AGG | - | chr4_1:19955916-19955935 | Msa0539240:CDS | 35.0% | |
| ! | ACATGATAAGGAAGCATACA+TGG | - | chr4_1:19956131-19956150 | Msa0539240:intron | 35.0% |
| ! | ACATGGTTGAGATTTTGTTG+AGG | - | chr4_1:19956148-19956167 | Msa0539240:intron | 35.0% |
| TGAGGTTCCAAATCATGTAA+AGG | - | chr4_1:19956166-19956185 | Msa0539240:intron | 35.0% | |
| !!! | CCCTTTTTTATTATGGTCCA+TGG | - | chr4_1:19956194-19956213 | Msa0539240:intron | 35.0% |
| ! | AGGCATTATAACGGAAAGTA+CGG | + | chr4_1:19956231-19956250 | None:intergenic | 35.0% |
| ! | TAGCACTTTAGTGCCATTAA+TGG | - | chr4_1:19956307-19956326 | Msa0539240:intron | 35.0% |
| AACACATTGTCATTGCATCT+AGG | - | chr4_1:19956358-19956377 | Msa0539240:intron | 35.0% | |
| !! | ATCTATCTTGGTGCCATTAA+TGG | - | chr4_1:19956392-19956411 | Msa0539240:intron | 35.0% |
| CATTAAATCTGGGCCATTAA+TGG | + | chr4_1:19956408-19956427 | None:intergenic | 35.0% | |
| CATTAATGGCCCAGATTTAA+TGG | - | chr4_1:19956406-19956425 | Msa0539240:intron | 35.0% | |
| AAAACCAAGTGTAAACGAAG+TGG | - | chr4_1:19956603-19956622 | Msa0539240:intron | 35.0% | |
| GCAAAATAGAGCAACAAGAT+TGG | + | chr4_1:19957050-19957069 | None:intergenic | 35.0% | |
| ! | TCTTGTTGCTCTATTTTGCA+TGG | - | chr4_1:19957051-19957070 | Msa0539240:intron | 35.0% |
| GATATGATGTCCATTGCTAT+GGG | + | chr4_1:19957143-19957162 | None:intergenic | 35.0% | |
| CATAGCAATGGACATCATAT+CGG | - | chr4_1:19957142-19957161 | Msa0539240:intron | 35.0% | |
| ATACACTTTGGACATGAACA+AGG | - | chr4_1:19957209-19957228 | Msa0539240:intron | 35.0% | |
| ! | AAGTGTTTCCTTCACTGATT+TGG | - | chr4_1:19957282-19957301 | Msa0539240:intron | 35.0% |
| ATCGTCTGGTAAAGATTAAG+CGG | - | chr4_1:19957330-19957349 | Msa0539240:intron | 35.0% | |
| AGCTAGGATTAATTTCCTGT+AGG | - | chr4_1:19957565-19957584 | Msa0539240:CDS | 35.0% | |
| AAAATCTTGCAAGCAGCACA+AGG | + | chr4_1:19955298-19955317 | None:intergenic | 40.0% | |
| GGATCAGTAGGAGGAAAATA+AGG | + | chr4_1:19955358-19955377 | None:intergenic | 40.0% | |
| !!! | GGTCCTGCCCTTTTTTATTA+TGG | - | chr4_1:19956187-19956206 | Msa0539240:intron | 40.0% |
| GGACCATAATAAAAAAGGGC+AGG | + | chr4_1:19956193-19956212 | None:intergenic | 40.0% | |
| ! | CATTATAACGGAAAGTACGG+GGG | + | chr4_1:19956228-19956247 | None:intergenic | 40.0% |
| ! | GCATTATAACGGAAAGTACG+GGG | + | chr4_1:19956229-19956248 | None:intergenic | 40.0% |
| ! | GGCATTATAACGGAAAGTAC+GGG | + | chr4_1:19956230-19956249 | None:intergenic | 40.0% |
| ATGACCCAGATTTAATGGCA+CGG | + | chr4_1:19956335-19956354 | None:intergenic | 40.0% | |
| ACCAAGATAGATGTGTTGTG+TGG | + | chr4_1:19956384-19956403 | None:intergenic | 40.0% | |
| ATGTATGACTACAAGGAGAG+AGG | + | chr4_1:19956479-19956498 | None:intergenic | 40.0% | |
| TTAGCCACTTCGTTTACACT+TGG | + | chr4_1:19956610-19956629 | None:intergenic | 40.0% | |
| !! | CTTGATAGCGACTGTTGATT+AGG | - | chr4_1:19956677-19956696 | Msa0539240:intron | 40.0% |
| AGTGACAAGCATGGTTTATG+AGG | - | chr4_1:19956892-19956911 | Msa0539240:intron | 40.0% | |
| AGAGTTAGAGACCCAGTTTA+TGG | - | chr4_1:19956923-19956942 | Msa0539240:intron | 40.0% | |
| !!! | GAAGCTCATTGACTTGCTTT+TGG | + | chr4_1:19956973-19956992 | None:intergenic | 40.0% |
| TACAACCTCAGCTTGTGATT+TGG | + | chr4_1:19957008-19957027 | None:intergenic | 40.0% | |
| ATCACCACAACAGTTATCAG+TGG | - | chr4_1:19957096-19957115 | Msa0539240:intron | 40.0% | |
| TCATCCACTGATAACTGTTG+TGG | + | chr4_1:19957103-19957122 | None:intergenic | 40.0% | |
| CGATATGATGTCCATTGCTA+TGG | + | chr4_1:19957144-19957163 | None:intergenic | 40.0% | |
| TTTGGACATGAACAAGGACA+AGG | - | chr4_1:19957215-19957234 | Msa0539240:intron | 40.0% | |
| CACACAAACCAAATCAGTGA+AGG | + | chr4_1:19957293-19957312 | None:intergenic | 40.0% | |
| ! | AATTTGATGACCATCTCCGA+AGG | - | chr4_1:19957490-19957509 | Msa0539240:CDS | 40.0% |
| AAAAGTAAGGTAGCAGAGCT+AGG | - | chr4_1:19957549-19957568 | Msa0539240:CDS | 40.0% | |
| !!! | GTGCTGCTTGCAAGATTTTG+AGG | - | chr4_1:19955299-19955318 | Msa0539240:three_prime_UTR | 45.0% |
| GTAAACTTGGCAGGATCAGT+AGG | + | chr4_1:19955370-19955389 | None:intergenic | 45.0% | |
| TGAGCAATGGTAAACTTGGC+AGG | + | chr4_1:19955379-19955398 | None:intergenic | 45.0% | |
| GGCAGGACCTTTACATGATT+TGG | + | chr4_1:19956176-19956195 | None:intergenic | 45.0% | |
| ! | ACTAAAGTGCTACCCCCATT+AGG | + | chr4_1:19956299-19956318 | None:intergenic | 45.0% |
| !! | TAATGGCACGGTGCCATTAA+TGG | + | chr4_1:19956423-19956442 | None:intergenic | 45.0% |
| GCCACACAACACATCTATCT+TGG | - | chr4_1:19956380-19956399 | Msa0539240:intron | 45.0% | |
| ATGGCCCAGATTTAATGGCA+CGG | - | chr4_1:19956411-19956430 | Msa0539240:intron | 45.0% | |
| !! | TAATGGCACGGTGCCATTAA+TGG | - | chr4_1:19956423-19956442 | Msa0539240:intron | 45.0% |
| !! | CTGCACTTTGGTGCCATTAA+TGG | + | chr4_1:19956439-19956458 | None:intergenic | 45.0% |
| CTCCTGCACAACCATAAACT+GGG | + | chr4_1:19956937-19956956 | None:intergenic | 45.0% | |
| ACTAGCCAAATCACAAGCTG+AGG | - | chr4_1:19957000-19957019 | Msa0539240:intron | 45.0% | |
| GGAAATGTCACAAAGTCCAC+AGG | - | chr4_1:19957072-19957091 | Msa0539240:intron | 45.0% | |
| AACTGTTGTGGTGATTCCTG+TGG | + | chr4_1:19957091-19957110 | None:intergenic | 45.0% | |
| CAAAGTCCTTCCCATAGCAA+TGG | - | chr4_1:19957130-19957149 | Msa0539240:intron | 45.0% | |
| TGATGTCCATTGCTATGGGA+AGG | + | chr4_1:19957139-19957158 | None:intergenic | 45.0% | |
| ! | ACAACACTAGCCTATGCTCA+GGG | - | chr4_1:19957182-19957201 | Msa0539240:intron | 45.0% |
| !! | CTGATTTGGTTTGTGTGCAC+CGG | - | chr4_1:19957296-19957315 | Msa0539240:intron | 45.0% |
| TTCCATTTCTCCTTCGGAGA+TGG | + | chr4_1:19957503-19957522 | None:intergenic | 45.0% | |
| AACTTGGCAGGATCAGTAGG+AGG | + | chr4_1:19955367-19955386 | None:intergenic | 50.0% | |
| ! | TGGCACCGTGCCATTAAATC+TGG | - | chr4_1:19956327-19956346 | Msa0539240:intron | 50.0% |
| ! | GGCACCGTGCCATTAAATCT+GGG | - | chr4_1:19956328-19956347 | Msa0539240:intron | 50.0% |
| ! | GGCACCGTGCCATTAAATCT+GGG | + | chr4_1:19956328-19956347 | None:intergenic | 50.0% |
| ! | TGGCACCGTGCCATTAAATC+TGG | + | chr4_1:19956327-19956346 | None:intergenic | 50.0% |
| CATTAGGTAGGGCTGCACTT+TGG | + | chr4_1:19956451-19956470 | None:intergenic | 50.0% | |
| CAAAGTGCAGCCCTACCTAA+TGG | - | chr4_1:19956449-19956468 | Msa0539240:intron | 50.0% | |
| GCATCAGCTCTTTGAGCTTC+TGG | + | chr4_1:19956872-19956891 | None:intergenic | 50.0% | |
| AGCTGATGCAGTGACAAGCA+TGG | - | chr4_1:19956883-19956902 | Msa0539240:intron | 50.0% | |
| GACCCAGTTTATGGTTGTGC+AGG | - | chr4_1:19956932-19956951 | Msa0539240:intron | 50.0% | |
| GCTCCTGCACAACCATAAAC+TGG | + | chr4_1:19956938-19956957 | None:intergenic | 50.0% | |
| !! | GAGCAACAAGATTGGCTTGC+TGG | + | chr4_1:19957042-19957061 | None:intergenic | 50.0% |
| ! | GACAACACTAGCCTATGCTC+AGG | - | chr4_1:19957181-19957200 | Msa0539240:intron | 50.0% |
| GACCATCTCCGAAGGAGAAA+TGG | - | chr4_1:19957498-19957517 | Msa0539240:CDS | 50.0% | |
| AGTGCAGCCCTACCTAATGG+TGG | - | chr4_1:19956452-19956471 | Msa0539240:intron | 55.0% | |
| GTGCAGCCCTACCTAATGGT+GGG | - | chr4_1:19956453-19956472 | Msa0539240:intron | 55.0% | |
| GAGAGGCCCACCATTAGGTA+GGG | + | chr4_1:19956462-19956481 | None:intergenic | 55.0% | |
| AGAGAGGCCCACCATTAGGT+AGG | + | chr4_1:19956463-19956482 | None:intergenic | 55.0% | |
| AAGGAGAGAGGCCCACCATT+AGG | + | chr4_1:19956467-19956486 | None:intergenic | 55.0% | |
| ! | ACACTAGCCTATGCTCAGGG+TGG | - | chr4_1:19957185-19957204 | Msa0539240:intron | 55.0% |
| ! | CACTAGCCTATGCTCAGGGT+GGG | - | chr4_1:19957186-19957205 | Msa0539240:intron | 55.0% |
| GTGTATCCCACCCTGAGCAT+AGG | + | chr4_1:19957195-19957214 | None:intergenic | 55.0% | |
| GCTCAGGGTGGGATACACTT+TGG | - | chr4_1:19957197-19957216 | Msa0539240:intron | 55.0% | |
| ! | GTTTGTGTGCACCGGCAATC+TGG | - | chr4_1:19957304-19957323 | Msa0539240:intron | 55.0% |
| CAGACGATGTGCCAGATTGC+CGG | + | chr4_1:19957318-19957337 | None:intergenic | 55.0% | |
| CACGCAGTCAGTCTCATCGA+TGG | + | chr4_1:19957439-19957458 | None:intergenic | 55.0% | |
| GTACGGGGGCTCCATATCCA+TGG | + | chr4_1:19956214-19956233 | None:intergenic | 60.0% | |
| CGGCAATCTGGCACATCGTC+TGG | - | chr4_1:19957316-19957335 | Msa0539240:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 19955174 | 19957678 | 19955174 | ID=Msa0539240;Name=Msa0539240 |
| chr4_1 | mRNA | 19955174 | 19957678 | 19955174 | ID=Msa0539240-mRNA-1;Parent=Msa0539240;Name=Msa0539240-mRNA-1;_AED=0.01;_eAED=0.01;_QI=16|1|1|1|1|1|2|453|203 |
| chr4_1 | exon | 19955174 | 19955989 | 19955174 | ID=Msa0539240-mRNA-1:exon:5730;Parent=Msa0539240-mRNA-1 |
| chr4_1 | exon | 19957414 | 19957678 | 19957414 | ID=Msa0539240-mRNA-1:exon:5729;Parent=Msa0539240-mRNA-1 |
| chr4_1 | five_prime_UTR | 19957663 | 19957678 | 19957663 | ID=Msa0539240-mRNA-1:five_prime_utr;Parent=Msa0539240-mRNA-1 |
| chr4_1 | CDS | 19957414 | 19957662 | 19957414 | ID=Msa0539240-mRNA-1:cds;Parent=Msa0539240-mRNA-1 |
| chr4_1 | CDS | 19955627 | 19955989 | 19955627 | ID=Msa0539240-mRNA-1:cds;Parent=Msa0539240-mRNA-1 |
| chr4_1 | three_prime_UTR | 19955174 | 19955626 | 19955174 | ID=Msa0539240-mRNA-1:three_prime_utr;Parent=Msa0539240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
