AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0683160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683160 MtrunA17_Chr4g0030001 92.147 191 8 2 19 203 1 190 2.35e-129 361
Msa0683160 MtrunA17_Chr1g0200201 72.024 168 35 6 37 203 73 229 1.63e-77 231
Msa0683160 MtrunA17_Chr2g0326051 69.841 126 37 1 78 203 1 125 4.15e-61 186
Msa0683160 MtrunA17_Chr2g0311601 72.321 112 30 1 33 144 10 120 2.84e-55 172
Msa0683160 MtrunA17_Chr3g0114061 56.000 150 57 3 35 182 7 149 6.84e-50 159
Msa0683160 MtrunA17_Chr4g0043421 66.364 110 36 1 35 144 14 122 2.65e-49 158
Msa0683160 MtrunA17_Chr5g0437571 64.035 114 40 1 35 148 5 117 4.53e-49 157
Msa0683160 MtrunA17_Chr3g0106101 63.636 110 39 1 35 144 6 114 3.67e-47 152
Msa0683160 MtrunA17_Chr5g0438091 56.140 114 49 1 35 148 38 150 1.41e-41 139
Msa0683160 MtrunA17_Chr3g0129071 55.752 113 49 1 33 145 44 155 4.51e-40 136
Msa0683160 MtrunA17_Chr6g0449641 49.180 122 61 1 29 150 2 122 1.62e-39 132
Msa0683160 MtrunA17_Chr7g0227511 54.955 111 49 1 28 138 3 112 2.48e-39 132
Msa0683160 MtrunA17_Chr3g0117121 55.455 110 48 1 35 144 9 117 2.85e-39 133
Msa0683160 MtrunA17_Chr4g0058651 56.481 108 46 1 35 142 10 116 7.92e-39 132
Msa0683160 MtrunA17_Chr3g0115431 56.311 103 44 1 35 137 8 109 5.48e-38 130
Msa0683160 MtrunA17_Chr6g0449651 43.357 143 67 2 35 177 66 194 1.29e-37 129
Msa0683160 MtrunA17_Chr5g0432451 57.522 113 47 1 33 145 40 151 3.17e-37 129
Msa0683160 MtrunA17_Chr5g0402611 53.153 111 51 1 35 145 7 116 7.03e-36 128
Msa0683160 MtrunA17_Chr7g0225111 47.321 112 58 1 32 143 1 111 1.12e-34 119
Msa0683160 MtrunA17_Chr3g0113891 57.732 97 40 1 35 131 13 108 5.31e-34 119
Msa0683160 MtrunA17_Chr3g0097221 50.926 108 52 1 35 142 6 112 1.50e-33 121
Msa0683160 MtrunA17_Chr5g0437401 56.701 97 41 1 35 131 10 105 3.39e-32 114
Msa0683160 MtrunA17_Chr6g0460951 44.118 136 66 4 33 166 37 164 7.69e-32 115
Msa0683160 MtrunA17_Chr7g0260971 52.885 104 47 2 35 137 13 115 9.25e-32 114
Msa0683160 MtrunA17_Chr8g0353981 60.976 82 31 1 35 116 6 86 1.32e-31 114
Msa0683160 MtrunA17_Chr5g0444351 41.964 112 64 1 32 143 1 111 6.20e-31 110
Msa0683160 MtrunA17_Chr1g0184271 40.854 164 80 6 36 193 12 164 5.25e-30 110
Msa0683160 MtrunA17_Chr1g0184281 48.214 112 55 3 36 145 15 125 1.28e-29 108
Msa0683160 MtrunA17_Chr8g0374601 46.667 105 55 1 33 137 23 126 2.80e-29 110
Msa0683160 MtrunA17_Chr8g0374591 41.732 127 70 2 33 156 5 130 3.73e-29 105
Msa0683160 MtrunA17_Chr7g0245871 49.515 103 50 2 36 137 27 128 5.66e-29 108
Msa0683160 MtrunA17_Chr2g0326041 73.611 72 14 2 16 83 9 79 8.17e-29 103
Msa0683160 MtrunA17_Chr8g0351721 49.074 108 53 2 35 141 17 123 2.37e-28 105
Msa0683160 MtrunA17_Chr8g0351701 46.789 109 56 2 35 142 22 129 3.82e-28 105
Msa0683160 MtrunA17_Chr5g0435641 45.378 119 56 3 35 145 6 123 8.33e-27 101
Msa0683160 MtrunA17_Chr7g0261031 52.041 98 45 2 35 131 7 103 1.38e-26 101
Msa0683160 MtrunA17_Chr8g0374581 43.396 106 60 0 33 138 5 110 3.53e-26 100
Msa0683160 MtrunA17_Chr7g0245861 41.525 118 67 1 35 150 11 128 2.01e-25 96.3
Msa0683160 MtrunA17_Chr6g0452691 35.714 168 89 6 37 199 10 163 4.07e-25 96.7
Msa0683160 MtrunA17_Chr6g0460941 44.944 89 47 2 36 123 7 94 1.41e-23 90.1
Msa0683160 MtrunA17_Chr4g0059901 43.617 94 52 1 37 130 11 103 1.24e-22 92.0
Msa0683160 MtrunA17_Chr6g0452661 42.149 121 58 4 37 149 10 126 1.37e-22 91.7
Msa0683160 MtrunA17_Chr6g0452671 48.750 80 40 1 37 116 12 90 1.18e-20 85.5
Msa0683160 MtrunA17_Chr4g0040831 31.210 157 97 4 25 180 4 150 1.41e-20 85.1
Msa0683160 MtrunA17_Chr4g0055441 43.750 96 48 3 37 127 3 97 1.47e-19 81.3
Msa0683160 MtrunA17_Chr2g0330201 37.624 101 62 1 37 137 20 119 3.65e-19 83.2
Msa0683160 MtrunA17_Chr8g0342351 41.489 94 54 1 37 130 13 105 1.37e-18 81.3
Msa0683160 MtrunA17_Chr5g0439111 45.679 81 42 2 37 116 10 89 8.55e-16 73.2
Msa0683160 MtrunA17_Chr4g0046201 34.483 87 56 1 37 123 7 92 9.86e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683160 AT2G28500.1 66.129 186 45 6 32 203 51 232 1.23e-75 227
Msa0683160 AT1G07900.1 62.500 176 47 4 32 203 29 189 5.14e-72 217
Msa0683160 AT2G30130.1 67.890 109 34 1 35 143 7 114 1.38e-49 160
Msa0683160 AT1G16530.1 58.716 109 45 0 33 141 11 119 1.45e-45 149
Msa0683160 AT1G31320.1 68.468 111 34 1 35 145 12 121 1.05e-43 144
Msa0683160 AT3G27650.1 58.654 104 42 1 35 138 38 140 6.72e-40 134
Msa0683160 AT5G63090.4 57.407 108 45 1 35 142 10 116 7.37e-40 135
Msa0683160 AT5G63090.3 57.407 108 45 1 35 142 10 116 7.37e-40 135
Msa0683160 AT5G63090.2 57.407 108 45 1 35 142 10 116 7.37e-40 135
Msa0683160 AT5G63090.1 57.407 108 45 1 35 142 10 116 7.37e-40 135
Msa0683160 AT2G30340.1 50.862 116 56 1 33 148 49 163 1.55e-39 136
Msa0683160 AT2G30340.2 50.862 116 56 1 33 148 50 164 1.71e-39 136
Msa0683160 AT3G26620.1 54.237 118 49 3 34 147 1 117 1.66e-38 129
Msa0683160 AT2G23660.2 55.238 105 46 1 35 139 4 107 1.85e-38 135
Msa0683160 AT2G23660.3 55.238 105 46 1 35 139 4 107 1.85e-38 135
Msa0683160 AT2G23660.1 55.238 105 46 1 35 139 4 107 1.85e-38 135
Msa0683160 AT3G26660.1 56.604 106 43 2 34 137 1 105 3.51e-38 128
Msa0683160 AT5G66870.1 51.128 133 62 2 35 167 6 135 8.01e-37 130
Msa0683160 AT2G40470.2 56.637 113 48 1 33 145 17 128 4.54e-36 125
Msa0683160 AT2G40470.1 56.637 113 48 1 33 145 42 153 6.60e-36 125
Msa0683160 AT3G11090.1 51.923 104 49 1 35 138 10 112 8.01e-33 116
Msa0683160 AT4G00210.1 52.778 108 49 2 35 141 10 116 1.49e-32 117
Msa0683160 AT1G65620.1 60.227 88 34 1 29 116 2 88 3.16e-32 115
Msa0683160 AT1G65620.4 60.227 88 34 1 29 116 2 88 3.16e-32 115
Msa0683160 AT1G65620.3 60.227 88 34 1 29 116 2 88 3.16e-32 115
Msa0683160 AT1G65620.5 60.227 88 34 1 29 116 2 88 3.16e-32 115
Msa0683160 AT1G65620.2 60.227 88 34 1 29 116 2 88 3.16e-32 115
Msa0683160 AT3G03760.1 47.788 113 57 2 35 146 50 161 3.12e-31 115
Msa0683160 AT2G42430.1 46.154 117 61 2 31 146 10 125 3.00e-29 109
Msa0683160 AT4G00220.1 49.524 105 51 2 39 142 20 123 8.76e-29 107
Msa0683160 AT2G45410.1 42.105 152 69 4 39 189 19 152 1.37e-28 106
Msa0683160 AT2G45420.1 47.619 105 53 2 39 142 40 143 1.09e-26 103
Msa0683160 AT2G42440.1 53.608 97 43 2 35 130 6 101 2.19e-26 102
Msa0683160 AT2G31310.1 49.485 97 47 2 35 130 6 101 2.25e-26 100
Msa0683160 AT3G58190.1 38.750 160 82 5 36 194 11 155 2.81e-26 100
Msa0683160 AT5G06080.1 43.363 113 62 2 35 146 6 117 2.99e-25 97.1
Msa0683160 AT3G47870.1 44.860 107 53 2 37 143 37 137 1.21e-24 99.0
Msa0683160 AT3G50510.1 41.441 111 64 1 27 137 3 112 5.10e-24 94.4
Msa0683160 AT3G50510.2 41.441 111 64 1 27 137 3 112 5.10e-24 94.4
Msa0683160 AT1G72980.1 36.232 138 77 3 26 163 5 131 4.21e-22 89.7
Msa0683160 AT5G35900.1 39.604 101 60 1 37 137 6 105 7.83e-22 89.0
Msa0683160 AT3G13850.1 35.256 156 84 5 37 187 37 180 5.16e-21 88.2
Msa0683160 AT1G06280.1 38.824 85 48 2 37 118 25 108 3.25e-15 71.2
Msa0683160 AT4G22700.1 37.864 103 59 4 37 137 6 105 3.80e-15 70.9
Msa0683160 AT2G19820.1 37.634 93 56 2 32 123 8 99 1.25e-13 65.5

Find 56 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCAGCTCTTTGAGCTTC+TGG 0.203584 4_4:-45597426 None:intergenic
GAAGCTCATTGACTTGCTTT+TGG 0.237617 4_4:-45597527 None:intergenic
AGAGTTAGAGACCCAGTTTA+TGG 0.300502 4_4:+45597480 Msa0683160:CDS
GAGCAACAAGATTGGCTTGC+TGG 0.325135 4_4:-45597596 None:intergenic
GCTCCTGCACAACCATAAAC+TGG 0.336614 4_4:-45597492 None:intergenic
CAACATCATCAAATTCTTAC+AGG 0.339119 4_4:+45596084 Msa0683160:CDS
TTAAACCGCTTAATCTTTAC+CGG 0.343274 4_4:-45597892 None:intergenic
TACAACCTCAGCTTGTGATT+TGG 0.347506 4_4:-45597562 None:intergenic
GTTTGTGTGCACCGGCAATC+TGG 0.378182 4_4:+45597861 Msa0683160:three_prime_UTR
GAGTGTTTCCTTCACTGATT+TGG 0.379887 4_4:+45597839 Msa0683160:three_prime_UTR
TGCTGCTGATAAATGTGTTT+TGG 0.380702 4_4:+45595997 Msa0683160:CDS
GCATTTGAAGTAATGGAATA+AGG 0.390026 4_4:-45595838 None:intergenic
AATATGATAGCATTACAAAT+AGG 0.395944 4_4:+45597807 Msa0683160:three_prime_UTR
GACCATCTCCGAAGGAGAAA+TGG 0.396372 4_4:+45598055 Msa0683160:three_prime_UTR
CATAGCAATGGACATCATAT+CGG 0.414964 4_4:+45597699 Msa0683160:CDS
GGATCAGTAGGAGGAAAATA+AGG 0.417860 4_4:-45596022 None:intergenic
GCAAAATAGAGCAACAAGAT+TGG 0.441636 4_4:-45597604 None:intergenic
CGGACGATGTGCCAGATTGC+CGG 0.446337 4_4:-45597872 None:intergenic
CGATATGATGTCCATTGCTA+TGG 0.449199 4_4:-45597698 None:intergenic
GACCCAGTTTATGGTTGTGC+AGG 0.466543 4_4:+45597489 Msa0683160:CDS
TTCCATTTCTCCTTCGGAGA+TGG 0.469366 4_4:-45598057 None:intergenic
CGGCAATCTGGCACATCGTC+CGG 0.473440 4_4:+45597873 Msa0683160:three_prime_UTR
TCTATGAGCAATGGTAAACT+TGG 0.485039 4_4:-45596047 None:intergenic
TGTATGTGCATTTGAAGTAA+TGG 0.489593 4_4:-45595845 None:intergenic
AAAAGTAAGGTAGCAGAGCT+AGG 0.502421 4_4:+45598106 Msa0683160:three_prime_UTR
ATATATAACTAATAAAAGTA+AGG 0.506193 4_4:+45598093 Msa0683160:three_prime_UTR
AGTGACAAGCATGGTTTATG+AGG 0.515235 4_4:+45597449 Msa0683160:CDS
TGAGCAATGGTAAACTTGGC+AGG 0.527769 4_4:-45596043 None:intergenic
GCTCAGGGTGGGATACACTT+TGG 0.537894 4_4:+45597754 Msa0683160:CDS
AGTGTTATTTATTAGTAACA+TGG 0.537934 4_4:+45595892 Msa0683160:CDS
CTGATTTGGTTTGTGTGCAC+CGG 0.542828 4_4:+45597853 Msa0683160:three_prime_UTR
GACAACACTAGCCTATGCTC+AGG 0.542861 4_4:+45597738 Msa0683160:CDS
CTCCTGCACAACCATAAACT+GGG 0.571360 4_4:-45597491 None:intergenic
GTAAACTTGGCAGGATCAGT+AGG 0.577739 4_4:-45596034 None:intergenic
AAAATCTTGCAAGCAGCACA+AGG 0.584368 4_4:-45595962 None:intergenic
TGATGTCCATTGCTATGGGA+AGG 0.587814 4_4:-45597693 None:intergenic
AACTGTTGTGGTGATTCCTG+TGG 0.589765 4_4:-45597645 None:intergenic
GTGTATCCCACCCTGAGCAT+AGG 0.591383 4_4:-45597749 None:intergenic
GATATGATGTCCATTGCTAT+GGG 0.597738 4_4:-45597697 None:intergenic
CACTAGCCTATGCTCAGGGT+GGG 0.599833 4_4:+45597743 Msa0683160:CDS
GGAAATGTCACAAAGTCCAC+AGG 0.603176 4_4:+45597629 Msa0683160:CDS
AGCTAGGATTAATTTCCTGT+AGG 0.610831 4_4:+45598122 Msa0683160:three_prime_UTR
CAAAGTCCTTCCCATAGCAA+TGG 0.614127 4_4:+45597687 Msa0683160:CDS
AGCTGATGCAGTGACAAGCA+TGG 0.615883 4_4:+45597440 Msa0683160:CDS
ATCGTCCGGTAAAGATTAAG+CGG 0.617463 4_4:+45597887 Msa0683160:three_prime_UTR
AATTTGATGACCATCTCCGA+AGG 0.635642 4_4:+45598047 Msa0683160:three_prime_UTR
CACACAAACCAAATCAGTGA+AGG 0.638489 4_4:-45597847 None:intergenic
AACTTGGCAGGATCAGTAGG+AGG 0.640755 4_4:-45596031 None:intergenic
ATACACTTTGGACATGAACA+AGG 0.645234 4_4:+45597766 Msa0683160:exon
ATCACCACAACAGTTATCAG+TGG 0.656563 4_4:+45597653 Msa0683160:CDS
ACTAGCCAAATCACAAGCTG+AGG 0.662044 4_4:+45597557 Msa0683160:CDS
CACGCAGTCAGTCTCATCGA+TGG 0.666062 4_4:-45597993 None:intergenic
ACAACACTAGCCTATGCTCA+GGG 0.673423 4_4:+45597739 Msa0683160:CDS
TCATCCACTGATAACTGTTG+TGG 0.694554 4_4:-45597657 None:intergenic
ACACTAGCCTATGCTCAGGG+TGG 0.697314 4_4:+45597742 Msa0683160:CDS
TTTGGACATGAACAAGGACA+AGG 0.723771 4_4:+45597772 Msa0683160:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAACTAATAAAAGTA+AGG + chr4_4:45598093-45598112 Msa0683160:three_prime_UTR 10.0%
!! ATATATAACTAATAAAAGTA+AGG + chr4_4:45598093-45598112 Msa0683160:three_prime_UTR 10.0%
!! AAACATATAGAAAAAAACAA+AGG + chr4_4:45595867-45595886 Msa0683160:CDS 15.0%
!! AACATATAGAAAAAAACAAA+GGG + chr4_4:45595868-45595887 Msa0683160:CDS 15.0%
!!! CAATTTTAATATTGATTTGT+AGG + chr4_4:45596485-45596504 Msa0683160:intron 15.0%
!! AAGATGGAATAATAATATAT+AGG + chr4_4:45596553-45596572 Msa0683160:intron 15.0%
!! GAAACAAAACAAAAAAATTT+AGG + chr4_4:45596828-45596847 Msa0683160:intron 15.0%
!!! TACTATTTGTTAGAATTATT+AGG - chr4_4:45597002-45597021 None:intergenic 15.0%
!!! TTTTTGTTGTTGAAAAAAAT+AGG + chr4_4:45597398-45597417 Msa0683160:intron 15.0%
!! AAACATATAGAAAAAAACAA+AGG + chr4_4:45595867-45595886 Msa0683160:CDS 15.0%
!! AACATATAGAAAAAAACAAA+GGG + chr4_4:45595868-45595887 Msa0683160:CDS 15.0%
!!! CAATTTTAATATTGATTTGT+AGG + chr4_4:45596485-45596504 Msa0683160:intron 15.0%
!! AAGATGGAATAATAATATAT+AGG + chr4_4:45596553-45596572 Msa0683160:intron 15.0%
!! GAAACAAAACAAAAAAATTT+AGG + chr4_4:45596828-45596847 Msa0683160:intron 15.0%
!!! TACTATTTGTTAGAATTATT+AGG - chr4_4:45597002-45597021 None:intergenic 15.0%
!!! TTTTTGTTGTTGAAAAAAAT+AGG + chr4_4:45597398-45597417 Msa0683160:intron 15.0%
!!! AGTGTTATTTATTAGTAACA+TGG + chr4_4:45595892-45595911 Msa0683160:CDS 20.0%
!! ACTAGAAAATGTTGAAATAT+TGG + chr4_4:45596118-45596137 Msa0683160:intron 20.0%
!! TATACATTCAACATTATCAA+AGG + chr4_4:45596746-45596765 Msa0683160:intron 20.0%
!!! ATTTATTTGAGGCATTATAA+CGG - chr4_4:45596966-45596985 None:intergenic 20.0%
!!! ATTCTTTAGCTTCTATTTTT+AGG - chr4_4:45597085-45597104 None:intergenic 20.0%
!! AAAAATAGAAGCTAAAGAAT+AGG + chr4_4:45597085-45597104 Msa0683160:intron 20.0%
!!! ATTTTTCTTGAAAAACCTAA+AGG + chr4_4:45597297-45597316 Msa0683160:intron 20.0%
!!! TTTTTTTTCCTTAAACCTTT+AGG - chr4_4:45597315-45597334 None:intergenic 20.0%
!! AATATGATAGCATTACAAAT+AGG + chr4_4:45597807-45597826 Msa0683160:three_prime_UTR 20.0%
!! GATGTACTATTAAAAAAAGT+TGG + chr4_4:45598169-45598188 Msa0683160:three_prime_UTR 20.0%
!!! AGTGTTATTTATTAGTAACA+TGG + chr4_4:45595892-45595911 Msa0683160:CDS 20.0%
!! ACTAGAAAATGTTGAAATAT+TGG + chr4_4:45596118-45596137 Msa0683160:intron 20.0%
!! TATACATTCAACATTATCAA+AGG + chr4_4:45596746-45596765 Msa0683160:intron 20.0%
!!! ATTTATTTGAGGCATTATAA+CGG - chr4_4:45596966-45596985 None:intergenic 20.0%
!!! ATTCTTTAGCTTCTATTTTT+AGG - chr4_4:45597085-45597104 None:intergenic 20.0%
!! AAAAATAGAAGCTAAAGAAT+AGG + chr4_4:45597085-45597104 Msa0683160:intron 20.0%
!!! ATTTTTCTTGAAAAACCTAA+AGG + chr4_4:45597297-45597316 Msa0683160:intron 20.0%
!!! TTTTTTTTCCTTAAACCTTT+AGG - chr4_4:45597315-45597334 None:intergenic 20.0%
!! AATATGATAGCATTACAAAT+AGG + chr4_4:45597807-45597826 Msa0683160:three_prime_UTR 20.0%
!! GATGTACTATTAAAAAAAGT+TGG + chr4_4:45598169-45598188 Msa0683160:three_prime_UTR 20.0%
! TTTGAATCAATGATGAAAGA+TGG + chr4_4:45596537-45596556 Msa0683160:intron 25.0%
! TTCTAACAAATAGTACCTAA+TGG + chr4_4:45597007-45597026 Msa0683160:intron 25.0%
!!! TCTATTTTTAGGTCAAGATT+TGG - chr4_4:45597074-45597093 None:intergenic 25.0%
! TAATCATGTATTTCAGAGTT+CGG - chr4_4:45597277-45597296 None:intergenic 25.0%
! TTGAAAAACCTAAAGGTTTA+AGG + chr4_4:45597304-45597323 Msa0683160:intron 25.0%
!! ATTTTTCTTTCGAGGAAAAA+TGG + chr4_4:45598147-45598166 Msa0683160:three_prime_UTR 25.0%
! TTTGAATCAATGATGAAAGA+TGG + chr4_4:45596537-45596556 Msa0683160:intron 25.0%
! TTCTAACAAATAGTACCTAA+TGG + chr4_4:45597007-45597026 Msa0683160:intron 25.0%
!!! TCTATTTTTAGGTCAAGATT+TGG - chr4_4:45597074-45597093 None:intergenic 25.0%
! TAATCATGTATTTCAGAGTT+CGG - chr4_4:45597277-45597296 None:intergenic 25.0%
! TTGAAAAACCTAAAGGTTTA+AGG + chr4_4:45597304-45597323 Msa0683160:intron 25.0%
!! ATTTTTCTTTCGAGGAAAAA+TGG + chr4_4:45598147-45598166 Msa0683160:three_prime_UTR 25.0%
! TGTATGTGCATTTGAAGTAA+TGG - chr4_4:45595848-45595867 None:intergenic 30.0%
CAACATCATCAAATTCTTAC+AGG + chr4_4:45596084-45596103 Msa0683160:CDS 30.0%
ATTGTTGTGATCTAAGTAAG+TGG + chr4_4:45596635-45596654 Msa0683160:intron 30.0%
GTATCAAGATTTACTTTGTG+AGG + chr4_4:45596712-45596731 Msa0683160:intron 30.0%
TTTAGGAGTGAAACATGATA+AGG + chr4_4:45596845-45596864 Msa0683160:intron 30.0%
CCATGGACCATAATAAAAAA+GGG - chr4_4:45596923-45596942 None:intergenic 30.0%
TCCATGGACCATAATAAAAA+AGG - chr4_4:45596924-45596943 None:intergenic 30.0%
TTTATTATGGTCCATGGATA+TGG + chr4_4:45596926-45596945 Msa0683160:intron 30.0%
GTTGACAATGCATTTATTTG+AGG - chr4_4:45596977-45596996 None:intergenic 30.0%
TAACAAATAGTACCTAATGG+TGG + chr4_4:45597010-45597029 Msa0683160:intron 30.0%
AACAAATAGTACCTAATGGT+GGG + chr4_4:45597011-45597030 Msa0683160:intron 30.0%
AAGTGTTATGTATGACTACA+AGG - chr4_4:45597044-45597063 None:intergenic 30.0%
AATAGAAGCTAAAGAATAGG+AGG + chr4_4:45597088-45597107 Msa0683160:intron 30.0%
TTAAACCGCTTAATCTTTAC+CGG - chr4_4:45597895-45597914 None:intergenic 30.0%
! TGATTTTTCCATTTCTCCTT+CGG - chr4_4:45598066-45598085 None:intergenic 30.0%
TCGAAAGAAAAATAACCTAC+AGG - chr4_4:45598140-45598159 None:intergenic 30.0%
!!! TGTAGGTTATTTTTCTTTCG+AGG + chr4_4:45598139-45598158 Msa0683160:three_prime_UTR 30.0%
! TTTTCATCTCGAATTGATGT+GGG - chr4_4:45598199-45598218 None:intergenic 30.0%
! TTTTTCATCTCGAATTGATG+TGG - chr4_4:45598200-45598219 None:intergenic 30.0%
! TGTATGTGCATTTGAAGTAA+TGG - chr4_4:45595848-45595867 None:intergenic 30.0%
CAACATCATCAAATTCTTAC+AGG + chr4_4:45596084-45596103 Msa0683160:CDS 30.0%
ATTGTTGTGATCTAAGTAAG+TGG + chr4_4:45596635-45596654 Msa0683160:intron 30.0%
GTATCAAGATTTACTTTGTG+AGG + chr4_4:45596712-45596731 Msa0683160:intron 30.0%
TTTAGGAGTGAAACATGATA+AGG + chr4_4:45596845-45596864 Msa0683160:intron 30.0%
CCATGGACCATAATAAAAAA+GGG - chr4_4:45596923-45596942 None:intergenic 30.0%
TCCATGGACCATAATAAAAA+AGG - chr4_4:45596924-45596943 None:intergenic 30.0%
TTTATTATGGTCCATGGATA+TGG + chr4_4:45596926-45596945 Msa0683160:intron 30.0%
GTTGACAATGCATTTATTTG+AGG - chr4_4:45596977-45596996 None:intergenic 30.0%
TAACAAATAGTACCTAATGG+TGG + chr4_4:45597010-45597029 Msa0683160:intron 30.0%
AACAAATAGTACCTAATGGT+GGG + chr4_4:45597011-45597030 Msa0683160:intron 30.0%
AAGTGTTATGTATGACTACA+AGG - chr4_4:45597044-45597063 None:intergenic 30.0%
AATAGAAGCTAAAGAATAGG+AGG + chr4_4:45597088-45597107 Msa0683160:intron 30.0%
TTAAACCGCTTAATCTTTAC+CGG - chr4_4:45597895-45597914 None:intergenic 30.0%
! TGATTTTTCCATTTCTCCTT+CGG - chr4_4:45598066-45598085 None:intergenic 30.0%
TCGAAAGAAAAATAACCTAC+AGG - chr4_4:45598140-45598159 None:intergenic 30.0%
!!! TGTAGGTTATTTTTCTTTCG+AGG + chr4_4:45598139-45598158 Msa0683160:three_prime_UTR 30.0%
! TTTTCATCTCGAATTGATGT+GGG - chr4_4:45598199-45598218 None:intergenic 30.0%
! TTTTTCATCTCGAATTGATG+TGG - chr4_4:45598200-45598219 None:intergenic 30.0%
! TGCTGCTGATAAATGTGTTT+TGG + chr4_4:45595997-45596016 Msa0683160:CDS 35.0%
TCTATGAGCAATGGTAAACT+TGG - chr4_4:45596050-45596069 None:intergenic 35.0%
! ACCATTGCTCATAGAGTTTT+TGG + chr4_4:45596055-45596074 Msa0683160:CDS 35.0%
ACCAAAAACTCTATGAGCAA+TGG - chr4_4:45596059-45596078 None:intergenic 35.0%
TGATCTAAGTAAGTGGTAAG+AGG + chr4_4:45596642-45596661 Msa0683160:intron 35.0%
! ACATGATAAGGAAGCATACA+TGG + chr4_4:45596857-45596876 Msa0683160:intron 35.0%
! ACATGGTTGAGATTTTGTTG+AGG + chr4_4:45596874-45596893 Msa0683160:intron 35.0%
TGAGGTTCCAAATCATGTAA+AGG + chr4_4:45596892-45596911 Msa0683160:intron 35.0%
!!! CCCTTTTTTATTATGGTCCA+TGG + chr4_4:45596920-45596939 Msa0683160:intron 35.0%
! AGGCATTATAACGGAAAGTA+CGG - chr4_4:45596957-45596976 None:intergenic 35.0%
AAAACCAAGTGTAAACGAAG+TGG + chr4_4:45597160-45597179 Msa0683160:intron 35.0%
GCAAAATAGAGCAACAAGAT+TGG - chr4_4:45597607-45597626 None:intergenic 35.0%
! TCTTGTTGCTCTATTTTGCA+TGG + chr4_4:45597608-45597627 Msa0683160:CDS 35.0%
GATATGATGTCCATTGCTAT+GGG - chr4_4:45597700-45597719 None:intergenic 35.0%
CATAGCAATGGACATCATAT+CGG + chr4_4:45597699-45597718 Msa0683160:CDS 35.0%
ATACACTTTGGACATGAACA+AGG + chr4_4:45597766-45597785 Msa0683160:exon 35.0%
AGCTAGGATTAATTTCCTGT+AGG + chr4_4:45598122-45598141 Msa0683160:three_prime_UTR 35.0%
! TGCTGCTGATAAATGTGTTT+TGG + chr4_4:45595997-45596016 Msa0683160:CDS 35.0%
TCTATGAGCAATGGTAAACT+TGG - chr4_4:45596050-45596069 None:intergenic 35.0%
! ACCATTGCTCATAGAGTTTT+TGG + chr4_4:45596055-45596074 Msa0683160:CDS 35.0%
ACCAAAAACTCTATGAGCAA+TGG - chr4_4:45596059-45596078 None:intergenic 35.0%
TGATCTAAGTAAGTGGTAAG+AGG + chr4_4:45596642-45596661 Msa0683160:intron 35.0%
! ACATGATAAGGAAGCATACA+TGG + chr4_4:45596857-45596876 Msa0683160:intron 35.0%
! ACATGGTTGAGATTTTGTTG+AGG + chr4_4:45596874-45596893 Msa0683160:intron 35.0%
TGAGGTTCCAAATCATGTAA+AGG + chr4_4:45596892-45596911 Msa0683160:intron 35.0%
!!! CCCTTTTTTATTATGGTCCA+TGG + chr4_4:45596920-45596939 Msa0683160:intron 35.0%
! AGGCATTATAACGGAAAGTA+CGG - chr4_4:45596957-45596976 None:intergenic 35.0%
AAAACCAAGTGTAAACGAAG+TGG + chr4_4:45597160-45597179 Msa0683160:intron 35.0%
GCAAAATAGAGCAACAAGAT+TGG - chr4_4:45597607-45597626 None:intergenic 35.0%
! TCTTGTTGCTCTATTTTGCA+TGG + chr4_4:45597608-45597627 Msa0683160:CDS 35.0%
GATATGATGTCCATTGCTAT+GGG - chr4_4:45597700-45597719 None:intergenic 35.0%
CATAGCAATGGACATCATAT+CGG + chr4_4:45597699-45597718 Msa0683160:CDS 35.0%
ATACACTTTGGACATGAACA+AGG + chr4_4:45597766-45597785 Msa0683160:exon 35.0%
AGCTAGGATTAATTTCCTGT+AGG + chr4_4:45598122-45598141 Msa0683160:three_prime_UTR 35.0%
AAAATCTTGCAAGCAGCACA+AGG - chr4_4:45595965-45595984 None:intergenic 40.0%
GGATCAGTAGGAGGAAAATA+AGG - chr4_4:45596025-45596044 None:intergenic 40.0%
!!! GGTCCTGCCCTTTTTTATTA+TGG + chr4_4:45596913-45596932 Msa0683160:intron 40.0%
GGACCATAATAAAAAAGGGC+AGG - chr4_4:45596919-45596938 None:intergenic 40.0%
! CATTATAACGGAAAGTACGG+GGG - chr4_4:45596954-45596973 None:intergenic 40.0%
! GCATTATAACGGAAAGTACG+GGG - chr4_4:45596955-45596974 None:intergenic 40.0%
! GGCATTATAACGGAAAGTAC+GGG - chr4_4:45596956-45596975 None:intergenic 40.0%
ATGTATGACTACAAGGAGAG+AGG - chr4_4:45597037-45597056 None:intergenic 40.0%
TTAGCCACTTCGTTTACACT+TGG - chr4_4:45597167-45597186 None:intergenic 40.0%
!! CTTGATAGCGACTGTTGATT+AGG + chr4_4:45597234-45597253 Msa0683160:intron 40.0%
AGTGACAAGCATGGTTTATG+AGG + chr4_4:45597449-45597468 Msa0683160:CDS 40.0%
AGAGTTAGAGACCCAGTTTA+TGG + chr4_4:45597480-45597499 Msa0683160:CDS 40.0%
!!! GAAGCTCATTGACTTGCTTT+TGG - chr4_4:45597530-45597549 None:intergenic 40.0%
TACAACCTCAGCTTGTGATT+TGG - chr4_4:45597565-45597584 None:intergenic 40.0%
ATCACCACAACAGTTATCAG+TGG + chr4_4:45597653-45597672 Msa0683160:CDS 40.0%
TCATCCACTGATAACTGTTG+TGG - chr4_4:45597660-45597679 None:intergenic 40.0%
CGATATGATGTCCATTGCTA+TGG - chr4_4:45597701-45597720 None:intergenic 40.0%
TTTGGACATGAACAAGGACA+AGG + chr4_4:45597772-45597791 Msa0683160:exon 40.0%
! GAGTGTTTCCTTCACTGATT+TGG + chr4_4:45597839-45597858 Msa0683160:three_prime_UTR 40.0%
CACACAAACCAAATCAGTGA+AGG - chr4_4:45597850-45597869 None:intergenic 40.0%
ATCGTCCGGTAAAGATTAAG+CGG + chr4_4:45597887-45597906 Msa0683160:three_prime_UTR 40.0%
! AATTTGATGACCATCTCCGA+AGG + chr4_4:45598047-45598066 Msa0683160:three_prime_UTR 40.0%
AAAAGTAAGGTAGCAGAGCT+AGG + chr4_4:45598106-45598125 Msa0683160:three_prime_UTR 40.0%
AAAATCTTGCAAGCAGCACA+AGG - chr4_4:45595965-45595984 None:intergenic 40.0%
GGATCAGTAGGAGGAAAATA+AGG - chr4_4:45596025-45596044 None:intergenic 40.0%
!!! GGTCCTGCCCTTTTTTATTA+TGG + chr4_4:45596913-45596932 Msa0683160:intron 40.0%
GGACCATAATAAAAAAGGGC+AGG - chr4_4:45596919-45596938 None:intergenic 40.0%
! CATTATAACGGAAAGTACGG+GGG - chr4_4:45596954-45596973 None:intergenic 40.0%
! GCATTATAACGGAAAGTACG+GGG - chr4_4:45596955-45596974 None:intergenic 40.0%
! GGCATTATAACGGAAAGTAC+GGG - chr4_4:45596956-45596975 None:intergenic 40.0%
ATGTATGACTACAAGGAGAG+AGG - chr4_4:45597037-45597056 None:intergenic 40.0%
TTAGCCACTTCGTTTACACT+TGG - chr4_4:45597167-45597186 None:intergenic 40.0%
!! CTTGATAGCGACTGTTGATT+AGG + chr4_4:45597234-45597253 Msa0683160:intron 40.0%
AGTGACAAGCATGGTTTATG+AGG + chr4_4:45597449-45597468 Msa0683160:CDS 40.0%
AGAGTTAGAGACCCAGTTTA+TGG + chr4_4:45597480-45597499 Msa0683160:CDS 40.0%
!!! GAAGCTCATTGACTTGCTTT+TGG - chr4_4:45597530-45597549 None:intergenic 40.0%
TACAACCTCAGCTTGTGATT+TGG - chr4_4:45597565-45597584 None:intergenic 40.0%
ATCACCACAACAGTTATCAG+TGG + chr4_4:45597653-45597672 Msa0683160:CDS 40.0%
TCATCCACTGATAACTGTTG+TGG - chr4_4:45597660-45597679 None:intergenic 40.0%
CGATATGATGTCCATTGCTA+TGG - chr4_4:45597701-45597720 None:intergenic 40.0%
TTTGGACATGAACAAGGACA+AGG + chr4_4:45597772-45597791 Msa0683160:exon 40.0%
! GAGTGTTTCCTTCACTGATT+TGG + chr4_4:45597839-45597858 Msa0683160:three_prime_UTR 40.0%
CACACAAACCAAATCAGTGA+AGG - chr4_4:45597850-45597869 None:intergenic 40.0%
ATCGTCCGGTAAAGATTAAG+CGG + chr4_4:45597887-45597906 Msa0683160:three_prime_UTR 40.0%
! AATTTGATGACCATCTCCGA+AGG + chr4_4:45598047-45598066 Msa0683160:three_prime_UTR 40.0%
AAAAGTAAGGTAGCAGAGCT+AGG + chr4_4:45598106-45598125 Msa0683160:three_prime_UTR 40.0%
!!! GTGCTGCTTGCAAGATTTTG+AGG + chr4_4:45595966-45595985 Msa0683160:CDS 45.0%
GTAAACTTGGCAGGATCAGT+AGG - chr4_4:45596037-45596056 None:intergenic 45.0%
TGAGCAATGGTAAACTTGGC+AGG - chr4_4:45596046-45596065 None:intergenic 45.0%
GGCAGGACCTTTACATGATT+TGG - chr4_4:45596902-45596921 None:intergenic 45.0%
CTCCTGCACAACCATAAACT+GGG - chr4_4:45597494-45597513 None:intergenic 45.0%
ACTAGCCAAATCACAAGCTG+AGG + chr4_4:45597557-45597576 Msa0683160:CDS 45.0%
GGAAATGTCACAAAGTCCAC+AGG + chr4_4:45597629-45597648 Msa0683160:CDS 45.0%
AACTGTTGTGGTGATTCCTG+TGG - chr4_4:45597648-45597667 None:intergenic 45.0%
CAAAGTCCTTCCCATAGCAA+TGG + chr4_4:45597687-45597706 Msa0683160:CDS 45.0%
TGATGTCCATTGCTATGGGA+AGG - chr4_4:45597696-45597715 None:intergenic 45.0%
! ACAACACTAGCCTATGCTCA+GGG + chr4_4:45597739-45597758 Msa0683160:CDS 45.0%
!! CTGATTTGGTTTGTGTGCAC+CGG + chr4_4:45597853-45597872 Msa0683160:three_prime_UTR 45.0%
TTCCATTTCTCCTTCGGAGA+TGG - chr4_4:45598060-45598079 None:intergenic 45.0%
!!! GTGCTGCTTGCAAGATTTTG+AGG + chr4_4:45595966-45595985 Msa0683160:CDS 45.0%
GTAAACTTGGCAGGATCAGT+AGG - chr4_4:45596037-45596056 None:intergenic 45.0%
TGAGCAATGGTAAACTTGGC+AGG - chr4_4:45596046-45596065 None:intergenic 45.0%
GGCAGGACCTTTACATGATT+TGG - chr4_4:45596902-45596921 None:intergenic 45.0%
CTCCTGCACAACCATAAACT+GGG - chr4_4:45597494-45597513 None:intergenic 45.0%
ACTAGCCAAATCACAAGCTG+AGG + chr4_4:45597557-45597576 Msa0683160:CDS 45.0%
GGAAATGTCACAAAGTCCAC+AGG + chr4_4:45597629-45597648 Msa0683160:CDS 45.0%
AACTGTTGTGGTGATTCCTG+TGG - chr4_4:45597648-45597667 None:intergenic 45.0%
CAAAGTCCTTCCCATAGCAA+TGG + chr4_4:45597687-45597706 Msa0683160:CDS 45.0%
TGATGTCCATTGCTATGGGA+AGG - chr4_4:45597696-45597715 None:intergenic 45.0%
! ACAACACTAGCCTATGCTCA+GGG + chr4_4:45597739-45597758 Msa0683160:CDS 45.0%
!! CTGATTTGGTTTGTGTGCAC+CGG + chr4_4:45597853-45597872 Msa0683160:three_prime_UTR 45.0%
TTCCATTTCTCCTTCGGAGA+TGG - chr4_4:45598060-45598079 None:intergenic 45.0%
AACTTGGCAGGATCAGTAGG+AGG - chr4_4:45596034-45596053 None:intergenic 50.0%
GCATCAGCTCTTTGAGCTTC+TGG - chr4_4:45597429-45597448 None:intergenic 50.0%
AGCTGATGCAGTGACAAGCA+TGG + chr4_4:45597440-45597459 Msa0683160:CDS 50.0%
GACCCAGTTTATGGTTGTGC+AGG + chr4_4:45597489-45597508 Msa0683160:CDS 50.0%
GCTCCTGCACAACCATAAAC+TGG - chr4_4:45597495-45597514 None:intergenic 50.0%
!! GAGCAACAAGATTGGCTTGC+TGG - chr4_4:45597599-45597618 None:intergenic 50.0%
! GACAACACTAGCCTATGCTC+AGG + chr4_4:45597738-45597757 Msa0683160:CDS 50.0%
GACCATCTCCGAAGGAGAAA+TGG + chr4_4:45598055-45598074 Msa0683160:three_prime_UTR 50.0%
AACTTGGCAGGATCAGTAGG+AGG - chr4_4:45596034-45596053 None:intergenic 50.0%
GCATCAGCTCTTTGAGCTTC+TGG - chr4_4:45597429-45597448 None:intergenic 50.0%
AGCTGATGCAGTGACAAGCA+TGG + chr4_4:45597440-45597459 Msa0683160:CDS 50.0%
GACCCAGTTTATGGTTGTGC+AGG + chr4_4:45597489-45597508 Msa0683160:CDS 50.0%
GCTCCTGCACAACCATAAAC+TGG - chr4_4:45597495-45597514 None:intergenic 50.0%
!! GAGCAACAAGATTGGCTTGC+TGG - chr4_4:45597599-45597618 None:intergenic 50.0%
! GACAACACTAGCCTATGCTC+AGG + chr4_4:45597738-45597757 Msa0683160:CDS 50.0%
GACCATCTCCGAAGGAGAAA+TGG + chr4_4:45598055-45598074 Msa0683160:three_prime_UTR 50.0%
AAGGAGAGAGGCCCACCATT+AGG - chr4_4:45597025-45597044 None:intergenic 55.0%
! ACACTAGCCTATGCTCAGGG+TGG + chr4_4:45597742-45597761 Msa0683160:CDS 55.0%
! CACTAGCCTATGCTCAGGGT+GGG + chr4_4:45597743-45597762 Msa0683160:CDS 55.0%
GTGTATCCCACCCTGAGCAT+AGG - chr4_4:45597752-45597771 None:intergenic 55.0%
GCTCAGGGTGGGATACACTT+TGG + chr4_4:45597754-45597773 Msa0683160:CDS 55.0%
! GTTTGTGTGCACCGGCAATC+TGG + chr4_4:45597861-45597880 Msa0683160:three_prime_UTR 55.0%
CACGCAGTCAGTCTCATCGA+TGG - chr4_4:45597996-45598015 None:intergenic 55.0%
AAGGAGAGAGGCCCACCATT+AGG - chr4_4:45597025-45597044 None:intergenic 55.0%
! ACACTAGCCTATGCTCAGGG+TGG + chr4_4:45597742-45597761 Msa0683160:CDS 55.0%
! CACTAGCCTATGCTCAGGGT+GGG + chr4_4:45597743-45597762 Msa0683160:CDS 55.0%
GTGTATCCCACCCTGAGCAT+AGG - chr4_4:45597752-45597771 None:intergenic 55.0%
GCTCAGGGTGGGATACACTT+TGG + chr4_4:45597754-45597773 Msa0683160:CDS 55.0%
! GTTTGTGTGCACCGGCAATC+TGG + chr4_4:45597861-45597880 Msa0683160:three_prime_UTR 55.0%
CACGCAGTCAGTCTCATCGA+TGG - chr4_4:45597996-45598015 None:intergenic 55.0%
GTACGGGGGCTCCATATCCA+TGG - chr4_4:45596940-45596959 None:intergenic 60.0%
CGGACGATGTGCCAGATTGC+CGG - chr4_4:45597875-45597894 None:intergenic 60.0%
CGGCAATCTGGCACATCGTC+CGG + chr4_4:45597873-45597892 Msa0683160:three_prime_UTR 60.0%
GTACGGGGGCTCCATATCCA+TGG - chr4_4:45596940-45596959 None:intergenic 60.0%
CGGACGATGTGCCAGATTGC+CGG - chr4_4:45597875-45597894 None:intergenic 60.0%
CGGCAATCTGGCACATCGTC+CGG + chr4_4:45597873-45597892 Msa0683160:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 45595841 45598235 45595841 ID=Msa0683160;Name=Msa0683160
chr4_4 mRNA 45595841 45598235 45595841 ID=Msa0683160-mRNA-1;Parent=Msa0683160;Name=Msa0683160-mRNA-1;_AED=0.01;_eAED=0.01;_QI=16|1|1|1|1|1|2|453|203
chr4_4 exon 45595841 45596105 45595841 ID=Msa0683160-mRNA-1:exon:11097;Parent=Msa0683160-mRNA-1
chr4_4 exon 45597420 45598235 45597420 ID=Msa0683160-mRNA-1:exon:11098;Parent=Msa0683160-mRNA-1
chr4_4 five_prime_UTR 45595841 45595856 45595841 ID=Msa0683160-mRNA-1:five_prime_utr;Parent=Msa0683160-mRNA-1
chr4_4 CDS 45595857 45596105 45595857 ID=Msa0683160-mRNA-1:cds;Parent=Msa0683160-mRNA-1
chr4_4 CDS 45597420 45597782 45597420 ID=Msa0683160-mRNA-1:cds;Parent=Msa0683160-mRNA-1
chr4_4 three_prime_UTR 45597783 45598235 45597783 ID=Msa0683160-mRNA-1:three_prime_utr;Parent=Msa0683160-mRNA-1
Gene Sequence

>Msa0683160

ATGCACATACAAACATATAGAAAAAAACAAAGGGAAGTGTTATTTATTAGTAACATGGAGAGTGCTACTAGTGACACTTTCTCTGCTCAAGCTGTTGTTATGAGTCCTTGTGCTGCTTGCAAGATTTTGAGGAGAAGATGTGCTGCTGATAAATGTGTTTTGGCACCTTATTTTCCTCCTACTGATCCTGCCAAGTTTACCATTGCTCATAGAGTTTTTGGTGCTAGCAACATCATCAAATTCTTACAGGAACTTCCAGAAGCTCAAAGAGCTGATGCAGTGACAAGCATGGTTTATGAGGCTAGTGCAAGAGTTAGAGACCCAGTTTATGGTTGTGCAGGAGCAATTTGTCAACTCCAAAAGCAAGTCAATGAGCTTCAAGCACAACTAGCCAAATCACAAGCTGAGGTTGTAAACATGAAACTCCAGCAAGCCAATCTTGTTGCTCTATTTTGCATGGAAATGTCACAAAGTCCACAGGAATCACCACAACAGTTATCAGTGGATGATTTCATTCAAAGTCCTTCCCATAGCAATGGACATCATATCGGTTTGAACTTTCTTGATGACAACACTAGCCTATGCTCAGGGTGGGATACACTTTGGACATGA
Protein sequence

>Msa0683160

MHIQTYRKKQREVLFISNMESATSDTFSAQAVVMSPCAACKILRRRCAADKCVLAPYFPPTDPAKFTIAHRVFGASNIIKFLQELPEAQRADAVTSMVYEASARVRDPVYGCAGAICQLQKQVNELQAQLAKSQAEVVNMKLQQANLVALFCMEMSQSPQESPQQLSVDDFIQSPSHSNGHHIGLNFLDDNTSLCSGWDTLWT*