AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0602350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0602350 MtrunA17_Chr4g0030001 92.147 191 8 2 1 185 1 190 2.47e-129 360
Msa0602350 MtrunA17_Chr1g0200201 72.024 168 35 6 19 185 73 229 1.68e-77 231
Msa0602350 MtrunA17_Chr2g0326051 69.841 126 37 1 60 185 1 125 2.71e-61 186
Msa0602350 MtrunA17_Chr2g0311601 72.321 112 30 1 15 126 10 120 1.68e-55 172
Msa0602350 MtrunA17_Chr3g0114061 56.000 150 57 3 17 164 7 149 3.55e-50 159
Msa0602350 MtrunA17_Chr4g0043421 66.364 110 36 1 17 126 14 122 1.73e-49 158
Msa0602350 MtrunA17_Chr5g0437571 64.035 114 40 1 17 130 5 117 2.03e-49 157
Msa0602350 MtrunA17_Chr3g0106101 63.636 110 39 1 17 126 6 114 1.90e-47 153
Msa0602350 MtrunA17_Chr5g0438091 56.140 114 49 1 17 130 38 150 6.27e-42 139
Msa0602350 MtrunA17_Chr3g0129071 55.752 113 49 1 15 127 44 155 3.33e-40 136
Msa0602350 MtrunA17_Chr6g0449641 49.180 122 61 1 11 132 2 122 7.65e-40 132
Msa0602350 MtrunA17_Chr7g0227511 54.955 111 49 1 10 120 3 112 1.22e-39 132
Msa0602350 MtrunA17_Chr3g0117121 55.455 110 48 1 17 126 9 117 1.62e-39 133
Msa0602350 MtrunA17_Chr4g0058651 56.481 108 46 1 17 124 10 116 4.13e-39 132
Msa0602350 MtrunA17_Chr3g0115431 56.311 103 44 1 17 119 8 109 2.44e-38 131
Msa0602350 MtrunA17_Chr6g0449651 43.357 143 67 2 17 159 66 194 6.42e-38 129
Msa0602350 MtrunA17_Chr5g0432451 57.522 113 47 1 15 127 40 151 1.68e-37 129
Msa0602350 MtrunA17_Chr5g0402611 53.153 111 51 1 17 127 7 116 3.89e-36 128
Msa0602350 MtrunA17_Chr7g0225111 47.321 112 58 1 14 125 1 111 5.97e-35 119
Msa0602350 MtrunA17_Chr3g0113891 57.732 97 40 1 17 113 13 108 2.52e-34 119
Msa0602350 MtrunA17_Chr3g0097221 50.926 108 52 1 17 124 6 112 6.93e-34 121
Msa0602350 MtrunA17_Chr5g0437401 56.701 97 41 1 17 113 10 105 1.85e-32 114
Msa0602350 MtrunA17_Chr6g0460951 44.118 136 66 4 15 148 37 164 4.18e-32 115
Msa0602350 MtrunA17_Chr7g0260971 52.885 104 47 2 17 119 13 115 4.52e-32 115
Msa0602350 MtrunA17_Chr8g0353981 60.976 82 31 1 17 98 6 86 7.09e-32 114
Msa0602350 MtrunA17_Chr5g0444351 41.964 112 64 1 14 125 1 111 3.13e-31 110
Msa0602350 MtrunA17_Chr1g0184271 40.854 164 80 6 18 175 12 164 2.68e-30 110
Msa0602350 MtrunA17_Chr1g0184281 48.214 112 55 3 18 127 15 125 7.52e-30 108
Msa0602350 MtrunA17_Chr8g0374601 46.667 105 55 1 15 119 23 126 1.16e-29 110
Msa0602350 MtrunA17_Chr8g0374591 41.732 127 70 2 15 138 5 130 1.95e-29 105
Msa0602350 MtrunA17_Chr7g0245871 49.515 103 50 2 18 119 27 128 3.10e-29 108
Msa0602350 MtrunA17_Chr2g0326041 87.273 55 6 1 11 65 26 79 7.54e-29 102
Msa0602350 MtrunA17_Chr8g0351721 49.074 108 53 2 17 123 17 123 1.39e-28 105
Msa0602350 MtrunA17_Chr8g0351701 46.789 109 56 2 17 124 22 129 1.79e-28 105
Msa0602350 MtrunA17_Chr5g0435641 45.378 119 56 3 17 127 6 123 4.95e-27 101
Msa0602350 MtrunA17_Chr7g0261031 52.041 98 45 2 17 113 7 103 8.85e-27 101
Msa0602350 MtrunA17_Chr8g0374581 43.396 106 60 0 15 120 5 110 1.26e-26 101
Msa0602350 MtrunA17_Chr7g0245861 41.525 118 67 1 17 132 11 128 1.28e-25 96.3
Msa0602350 MtrunA17_Chr6g0452691 35.714 168 89 6 19 181 10 163 3.10e-25 96.3
Msa0602350 MtrunA17_Chr6g0460941 44.944 89 47 2 18 105 7 94 7.48e-24 90.1
Msa0602350 MtrunA17_Chr4g0059901 43.617 94 52 1 19 112 11 103 5.99e-23 92.4
Msa0602350 MtrunA17_Chr6g0452661 42.149 121 58 4 19 131 10 126 6.23e-23 92.0
Msa0602350 MtrunA17_Chr4g0040831 32.215 149 90 4 15 162 12 150 6.49e-21 85.5
Msa0602350 MtrunA17_Chr6g0452671 42.553 94 53 1 19 112 12 104 7.53e-21 85.5
Msa0602350 MtrunA17_Chr4g0055441 44.211 95 47 3 19 108 3 96 7.75e-20 81.6
Msa0602350 MtrunA17_Chr2g0330201 37.624 101 62 1 19 119 20 119 1.40e-19 84.0
Msa0602350 MtrunA17_Chr8g0342351 41.489 94 54 1 19 112 13 105 5.93e-19 81.6
Msa0602350 MtrunA17_Chr5g0439111 45.679 81 42 2 19 98 10 89 5.46e-16 73.2
Msa0602350 MtrunA17_Chr4g0046201 34.483 87 56 1 19 105 7 92 4.41e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0602350 AT2G28500.1 66.129 186 45 6 14 185 51 232 1.03e-75 227
Msa0602350 AT1G07900.1 62.500 176 47 4 14 185 29 189 2.31e-72 217
Msa0602350 AT2G30130.1 67.890 109 34 1 17 125 7 114 7.97e-50 160
Msa0602350 AT1G16530.1 58.716 109 45 0 15 123 11 119 6.27e-46 149
Msa0602350 AT1G31320.1 68.468 111 34 1 17 127 12 121 5.82e-44 144
Msa0602350 AT5G63090.4 57.407 108 45 1 17 124 10 116 3.71e-40 135
Msa0602350 AT5G63090.3 57.407 108 45 1 17 124 10 116 3.71e-40 135
Msa0602350 AT5G63090.2 57.407 108 45 1 17 124 10 116 3.71e-40 135
Msa0602350 AT5G63090.1 57.407 108 45 1 17 124 10 116 3.71e-40 135
Msa0602350 AT3G27650.1 58.654 104 42 1 17 120 38 140 3.77e-40 134
Msa0602350 AT2G30340.1 50.862 116 56 1 15 130 49 163 7.07e-40 136
Msa0602350 AT2G30340.2 50.862 116 56 1 15 130 50 164 7.56e-40 136
Msa0602350 AT2G23660.2 55.238 105 46 1 17 121 4 107 9.71e-39 135
Msa0602350 AT2G23660.3 55.238 105 46 1 17 121 4 107 9.71e-39 135
Msa0602350 AT2G23660.1 55.238 105 46 1 17 121 4 107 9.71e-39 135
Msa0602350 AT3G26620.1 54.237 118 49 3 16 129 1 117 1.05e-38 129
Msa0602350 AT3G26660.1 56.604 106 43 2 16 119 1 105 2.70e-38 128
Msa0602350 AT5G66870.1 51.128 133 62 2 17 149 6 135 4.56e-37 130
Msa0602350 AT2G40470.2 57.143 112 47 1 16 127 18 128 2.08e-36 125
Msa0602350 AT2G40470.1 56.637 113 48 1 15 127 42 153 3.75e-36 125
Msa0602350 AT3G11090.1 51.923 104 49 1 17 120 10 112 3.58e-33 116
Msa0602350 AT4G00210.1 52.778 108 49 2 17 123 10 116 6.74e-33 117
Msa0602350 AT1G65620.1 60.227 88 34 1 11 98 2 88 2.00e-32 115
Msa0602350 AT1G65620.4 60.227 88 34 1 11 98 2 88 2.00e-32 115
Msa0602350 AT1G65620.3 60.227 88 34 1 11 98 2 88 2.00e-32 115
Msa0602350 AT1G65620.5 60.227 88 34 1 11 98 2 88 2.00e-32 115
Msa0602350 AT1G65620.2 60.227 88 34 1 11 98 2 88 2.00e-32 115
Msa0602350 AT3G03760.1 47.788 113 57 2 17 128 50 161 1.68e-31 115
Msa0602350 AT2G42430.1 46.154 117 61 2 13 128 10 125 1.90e-29 109
Msa0602350 AT4G00220.1 49.524 105 51 2 21 124 20 123 3.75e-29 108
Msa0602350 AT2G45410.1 42.105 152 69 4 21 171 19 152 5.70e-29 106
Msa0602350 AT2G45420.1 47.619 105 53 2 21 124 40 143 4.60e-27 103
Msa0602350 AT2G42440.1 53.608 97 43 2 17 112 6 101 1.02e-26 102
Msa0602350 AT2G31310.1 47.573 103 52 2 17 118 6 107 1.16e-26 100
Msa0602350 AT3G58190.1 38.125 160 83 5 18 176 11 155 1.62e-26 100
Msa0602350 AT5G06080.1 43.363 113 62 2 17 128 6 117 1.21e-25 97.4
Msa0602350 AT3G47870.1 44.860 107 53 2 19 125 37 137 6.88e-25 99.0
Msa0602350 AT3G50510.1 41.441 111 64 1 9 119 3 112 3.02e-24 94.4
Msa0602350 AT3G50510.2 41.441 111 64 1 9 119 3 112 3.02e-24 94.4
Msa0602350 AT1G72980.1 36.232 138 77 3 8 145 5 131 1.93e-22 90.1
Msa0602350 AT5G35900.1 39.604 101 60 1 19 119 6 105 4.40e-22 89.0
Msa0602350 AT3G13850.1 35.256 156 84 5 19 169 37 180 2.13e-21 88.6
Msa0602350 AT1G06280.1 38.824 85 48 2 19 100 25 108 1.91e-15 71.6
Msa0602350 AT4G22700.1 37.864 103 59 4 19 119 6 105 2.88e-15 70.9
Msa0602350 AT2G19820.1 37.634 93 56 2 14 105 8 99 9.80e-14 65.1

Find 55 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCAGCTCTTTGAGCTTC+TGG 0.203584 4_2:+45909641 None:intergenic
GAAGCTCATTGACTTGCTTT+TGG 0.237617 4_2:+45909540 None:intergenic
AGAGTTAGAGACCCAGTTTA+TGG 0.300502 4_2:-45909587 Msa0602350:CDS
AAGTGTTTCCTTCACTGATT+TGG 0.314043 4_2:-45909228 Msa0602350:three_prime_UTR
GAGCAACAAGATTGGCTTGC+TGG 0.325135 4_2:+45909471 None:intergenic
GCTCCTGCACAACCATAAAC+TGG 0.336614 4_2:+45909575 None:intergenic
CAACATCATCAAATTCTTAC+AGG 0.339119 4_2:-45910980 Msa0602350:intron
TACAACCTCAGCTTGTGATT+TGG 0.347506 4_2:+45909505 None:intergenic
GTTTGTGTGCACCGGCAATC+TGG 0.378182 4_2:-45909206 Msa0602350:three_prime_UTR
TGCTGCTGATAAATGTGTTT+TGG 0.380702 4_2:-45911067 Msa0602350:CDS
GCATTTGAAGTAATGGAATA+AGG 0.390026 4_2:+45911227 None:intergenic
AATATGATAGCATTACAAAT+AGG 0.395944 4_2:-45909260 Msa0602350:three_prime_UTR
GACCATCTCCGAAGGAGAAA+TGG 0.396372 4_2:-45909012 Msa0602350:three_prime_UTR
CATAGCAATGGACATCATAT+CGG 0.414964 4_2:-45909368 Msa0602350:CDS
GGATCAGTAGGAGGAAAATA+AGG 0.417860 4_2:+45911042 None:intergenic
GCAAAATAGAGCAACAAGAT+TGG 0.441636 4_2:+45909463 None:intergenic
CAGACGATGTGCCAGATTGC+CGG 0.444100 4_2:+45909195 None:intergenic
CGGCAATCTGGCACATCGTC+TGG 0.445330 4_2:-45909194 Msa0602350:three_prime_UTR
CGATATGATGTCCATTGCTA+TGG 0.449199 4_2:+45909369 None:intergenic
GACCCAGTTTATGGTTGTGC+AGG 0.466543 4_2:-45909578 Msa0602350:CDS
TTCCATTTCTCCTTCGGAGA+TGG 0.469366 4_2:+45909010 None:intergenic
TCTATGAGCAATGGTAAACT+TGG 0.485039 4_2:+45911017 None:intergenic
TGTATGTGCATTTGAAGTAA+TGG 0.489593 4_2:+45911220 None:intergenic
AAAAGTAAGGTAGCAGAGCT+AGG 0.502421 4_2:-45908961 Msa0602350:three_prime_UTR
ATATATAACTAATAAAAGTA+AGG 0.506193 4_2:-45908974 Msa0602350:three_prime_UTR
AGTGACAAGCATGGTTTATG+AGG 0.515235 4_2:-45909618 Msa0602350:CDS
TGAGCAATGGTAAACTTGGC+AGG 0.527769 4_2:+45911021 None:intergenic
GCTCAGGGTGGGATACACTT+TGG 0.537894 4_2:-45909313 Msa0602350:CDS
AGTGTTATTTATTAGTAACA+TGG 0.537934 4_2:-45911172 Msa0602350:exon
CTGATTTGGTTTGTGTGCAC+CGG 0.542828 4_2:-45909214 Msa0602350:three_prime_UTR
GACAACACTAGCCTATGCTC+AGG 0.542861 4_2:-45909329 Msa0602350:CDS
CTCCTGCACAACCATAAACT+GGG 0.571360 4_2:+45909576 None:intergenic
GTAAACTTGGCAGGATCAGT+AGG 0.577739 4_2:+45911030 None:intergenic
AAAATCTTGCAAGCAGCACA+AGG 0.584368 4_2:+45911102 None:intergenic
TGATGTCCATTGCTATGGGA+AGG 0.587814 4_2:+45909374 None:intergenic
GTGTATCCCACCCTGAGCAT+AGG 0.591383 4_2:+45909318 None:intergenic
AATTGTTGTGGTGATTCCTG+TGG 0.595182 4_2:+45909422 None:intergenic
GATATGATGTCCATTGCTAT+GGG 0.597738 4_2:+45909370 None:intergenic
CACTAGCCTATGCTCAGGGT+GGG 0.599833 4_2:-45909324 Msa0602350:CDS
ATCGTCTGGTAAAGATTAAG+CGG 0.601837 4_2:-45909180 Msa0602350:three_prime_UTR
GGAAATGTCACAAAGTCCAC+AGG 0.603176 4_2:-45909438 Msa0602350:CDS
AGCTAGGATTAATTTCCTGT+AGG 0.610831 4_2:-45908945 Msa0602350:three_prime_UTR
CAAAGTCCTTCCCATAGCAA+TGG 0.614127 4_2:-45909380 Msa0602350:CDS
AGCTGATGCAGTGACAAGCA+TGG 0.615883 4_2:-45909627 Msa0602350:CDS
AATTTGATGACCATCTCCGA+AGG 0.635642 4_2:-45909020 Msa0602350:three_prime_UTR
CACACAAACCAAATCAGTGA+AGG 0.638489 4_2:+45909220 None:intergenic
AACTTGGCAGGATCAGTAGG+AGG 0.640755 4_2:+45911033 None:intergenic
TCATCCACTGATAATTGTTG+TGG 0.644577 4_2:+45909410 None:intergenic
ATACACTTTGGACATGAACA+AGG 0.645234 4_2:-45909301 Msa0602350:exon
ATCACCACAACAATTATCAG+TGG 0.648228 4_2:-45909414 Msa0602350:CDS
ACTAGCCAAATCACAAGCTG+AGG 0.662044 4_2:-45909510 Msa0602350:CDS
CACGCAGTCAGTCTCATCGA+TGG 0.666062 4_2:+45909074 None:intergenic
ACAACACTAGCCTATGCTCA+GGG 0.673423 4_2:-45909328 Msa0602350:CDS
ACACTAGCCTATGCTCAGGG+TGG 0.697314 4_2:-45909325 Msa0602350:CDS
TTTGGACATGAACAAGGACA+AGG 0.723771 4_2:-45909295 Msa0602350:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAACTAATAAAAGTA+AGG - chr4_2:45911104-45911123 Msa0602350:CDS 10.0%
!! ATATATAACTAATAAAAGTA+AGG - chr4_2:45911104-45911123 Msa0602350:CDS 10.0%
!! AACATATAGAAAAAAAACAA+AGG - chr4_2:45908881-45908900 Msa0602350:three_prime_UTR 15.0%
!! ACATATAGAAAAAAAACAAA+GGG - chr4_2:45908882-45908901 Msa0602350:three_prime_UTR 15.0%
!!! CAATTTTAATATTGATTTGT+AGG - chr4_2:45909499-45909518 Msa0602350:CDS 15.0%
!! AAGATGGAATAATAATATAT+AGG - chr4_2:45909567-45909586 Msa0602350:CDS 15.0%
!! GAAACAAAACAAAAAAATTT+AGG - chr4_2:45909842-45909861 Msa0602350:intron 15.0%
!!! TACTATTTGTTAGAATTATT+AGG + chr4_2:45910016-45910035 None:intergenic 15.0%
!!! TTTTTGTTGTTGAAAAAAAT+AGG - chr4_2:45910409-45910428 Msa0602350:intron 15.0%
!! AACATATAGAAAAAAAACAA+AGG - chr4_2:45908881-45908900 Msa0602350:three_prime_UTR 15.0%
!! ACATATAGAAAAAAAACAAA+GGG - chr4_2:45908882-45908901 Msa0602350:three_prime_UTR 15.0%
!!! CAATTTTAATATTGATTTGT+AGG - chr4_2:45909499-45909518 Msa0602350:CDS 15.0%
!! AAGATGGAATAATAATATAT+AGG - chr4_2:45909567-45909586 Msa0602350:CDS 15.0%
!! GAAACAAAACAAAAAAATTT+AGG - chr4_2:45909842-45909861 Msa0602350:intron 15.0%
!!! TACTATTTGTTAGAATTATT+AGG + chr4_2:45910016-45910035 None:intergenic 15.0%
!!! TTTTTGTTGTTGAAAAAAAT+AGG - chr4_2:45910409-45910428 Msa0602350:intron 15.0%
!!! AGTGTTATTTATTAGTAACA+TGG - chr4_2:45908906-45908925 Msa0602350:three_prime_UTR 20.0%
!! ACTAGAAAATGTTGAAATAT+CGG - chr4_2:45909132-45909151 Msa0602350:three_prime_UTR 20.0%
!! TATACATTCAACATTATCAA+AGG - chr4_2:45909760-45909779 Msa0602350:intron 20.0%
!!! ATTTATTTGAGGCATTATAA+CGG + chr4_2:45909980-45909999 None:intergenic 20.0%
!!! TATTTGTTAGAATTATTAGG+TGG + chr4_2:45910013-45910032 None:intergenic 20.0%
!!! ATTCTTTAGCTTCTATTTTT+AGG + chr4_2:45910099-45910118 None:intergenic 20.0%
!! AAAAATAGAAGCTAAAGAAT+AGG - chr4_2:45910099-45910118 Msa0602350:intron 20.0%
!!! TTTTTTTTCCTTAAACCTTT+CGG + chr4_2:45910328-45910347 None:intergenic 20.0%
!! AATATGATAGCATTACAAAT+AGG - chr4_2:45910818-45910837 Msa0602350:intron 20.0%
!! GATGTACTATTAAAAAAAGT+TGG - chr4_2:45911180-45911199 Msa0602350:five_prime_UTR 20.0%
!!! AGTGTTATTTATTAGTAACA+TGG - chr4_2:45908906-45908925 Msa0602350:three_prime_UTR 20.0%
!! ACTAGAAAATGTTGAAATAT+CGG - chr4_2:45909132-45909151 Msa0602350:three_prime_UTR 20.0%
!! TATACATTCAACATTATCAA+AGG - chr4_2:45909760-45909779 Msa0602350:intron 20.0%
!!! ATTTATTTGAGGCATTATAA+CGG + chr4_2:45909980-45909999 None:intergenic 20.0%
!!! TATTTGTTAGAATTATTAGG+TGG + chr4_2:45910013-45910032 None:intergenic 20.0%
!!! ATTCTTTAGCTTCTATTTTT+AGG + chr4_2:45910099-45910118 None:intergenic 20.0%
!! AAAAATAGAAGCTAAAGAAT+AGG - chr4_2:45910099-45910118 Msa0602350:intron 20.0%
!!! TTTTTTTTCCTTAAACCTTT+CGG + chr4_2:45910328-45910347 None:intergenic 20.0%
!! AATATGATAGCATTACAAAT+AGG - chr4_2:45910818-45910837 Msa0602350:intron 20.0%
!! GATGTACTATTAAAAAAAGT+TGG - chr4_2:45911180-45911199 Msa0602350:five_prime_UTR 20.0%
! TTTGAATCAATGATGAAAGA+TGG - chr4_2:45909551-45909570 Msa0602350:CDS 25.0%
!!! GTATCAAGATTTACTTTTTG+AGG - chr4_2:45909726-45909745 Msa0602350:intron 25.0%
! TTCTAACAAATAGTACCTAA+TGG - chr4_2:45910021-45910040 Msa0602350:intron 25.0%
!!! TCTATTTTTAGGTCAAGATT+TGG + chr4_2:45910088-45910107 None:intergenic 25.0%
!! ATTTTTCTTTCGAGGAAAAA+TGG - chr4_2:45911158-45911177 Msa0602350:exon 25.0%
! TTTGAATCAATGATGAAAGA+TGG - chr4_2:45909551-45909570 Msa0602350:CDS 25.0%
!!! GTATCAAGATTTACTTTTTG+AGG - chr4_2:45909726-45909745 Msa0602350:intron 25.0%
! TTCTAACAAATAGTACCTAA+TGG - chr4_2:45910021-45910040 Msa0602350:intron 25.0%
!!! TCTATTTTTAGGTCAAGATT+TGG + chr4_2:45910088-45910107 None:intergenic 25.0%
!! ATTTTTCTTTCGAGGAAAAA+TGG - chr4_2:45911158-45911177 Msa0602350:exon 25.0%
! TGTATGTGCATTTGAAGTAA+TGG + chr4_2:45908861-45908880 None:intergenic 30.0%
CAACATCATCAAATTCTTAC+AGG - chr4_2:45909098-45909117 Msa0602350:three_prime_UTR 30.0%
ATTGTTGTGATCTAAGTAAG+TGG - chr4_2:45909649-45909668 Msa0602350:CDS 30.0%
TTTAGGAGTGAAACATGATA+AGG - chr4_2:45909859-45909878 Msa0602350:intron 30.0%
CCATGGACCATAATAAAAAA+GGG + chr4_2:45909937-45909956 None:intergenic 30.0%
TCCATGGACCATAATAAAAA+AGG + chr4_2:45909938-45909957 None:intergenic 30.0%
TTTATTATGGTCCATGGATA+TGG - chr4_2:45909940-45909959 Msa0602350:intron 30.0%
GTTGACAATGCATTTATTTG+AGG + chr4_2:45909991-45910010 None:intergenic 30.0%
TAACAAATAGTACCTAATGG+TGG - chr4_2:45910024-45910043 Msa0602350:intron 30.0%
AACAAATAGTACCTAATGGT+GGG - chr4_2:45910025-45910044 Msa0602350:intron 30.0%
AAGTGTTATGTATGACTACA+AGG + chr4_2:45910058-45910077 None:intergenic 30.0%
AATAGAAGCTAAAGAATAGG+AGG - chr4_2:45910102-45910121 Msa0602350:intron 30.0%
TAGTCATGTATTTCAGAGTT+CGG + chr4_2:45910290-45910309 None:intergenic 30.0%
! ATTTTTCTCGAAAAACCGAA+AGG - chr4_2:45910310-45910329 Msa0602350:intron 30.0%
! TGATTTTTCCATTTCTCCTT+CGG + chr4_2:45911077-45911096 None:intergenic 30.0%
TCGAAAGAAAAATAACCTAC+AGG + chr4_2:45911151-45911170 None:intergenic 30.0%
!!! TGTAGGTTATTTTTCTTTCG+AGG - chr4_2:45911150-45911169 Msa0602350:CDS 30.0%
! TTTTCATCTCGAATTGATGT+GGG + chr4_2:45911210-45911229 None:intergenic 30.0%
! TTTTTCATCTCGAATTGATG+TGG + chr4_2:45911211-45911230 None:intergenic 30.0%
! TGTATGTGCATTTGAAGTAA+TGG + chr4_2:45908861-45908880 None:intergenic 30.0%
CAACATCATCAAATTCTTAC+AGG - chr4_2:45909098-45909117 Msa0602350:three_prime_UTR 30.0%
ATTGTTGTGATCTAAGTAAG+TGG - chr4_2:45909649-45909668 Msa0602350:CDS 30.0%
TTTAGGAGTGAAACATGATA+AGG - chr4_2:45909859-45909878 Msa0602350:intron 30.0%
CCATGGACCATAATAAAAAA+GGG + chr4_2:45909937-45909956 None:intergenic 30.0%
TCCATGGACCATAATAAAAA+AGG + chr4_2:45909938-45909957 None:intergenic 30.0%
TTTATTATGGTCCATGGATA+TGG - chr4_2:45909940-45909959 Msa0602350:intron 30.0%
GTTGACAATGCATTTATTTG+AGG + chr4_2:45909991-45910010 None:intergenic 30.0%
TAACAAATAGTACCTAATGG+TGG - chr4_2:45910024-45910043 Msa0602350:intron 30.0%
AACAAATAGTACCTAATGGT+GGG - chr4_2:45910025-45910044 Msa0602350:intron 30.0%
AAGTGTTATGTATGACTACA+AGG + chr4_2:45910058-45910077 None:intergenic 30.0%
AATAGAAGCTAAAGAATAGG+AGG - chr4_2:45910102-45910121 Msa0602350:intron 30.0%
TAGTCATGTATTTCAGAGTT+CGG + chr4_2:45910290-45910309 None:intergenic 30.0%
! ATTTTTCTCGAAAAACCGAA+AGG - chr4_2:45910310-45910329 Msa0602350:intron 30.0%
! TGATTTTTCCATTTCTCCTT+CGG + chr4_2:45911077-45911096 None:intergenic 30.0%
TCGAAAGAAAAATAACCTAC+AGG + chr4_2:45911151-45911170 None:intergenic 30.0%
!!! TGTAGGTTATTTTTCTTTCG+AGG - chr4_2:45911150-45911169 Msa0602350:CDS 30.0%
! TTTTCATCTCGAATTGATGT+GGG + chr4_2:45911210-45911229 None:intergenic 30.0%
! TTTTTCATCTCGAATTGATG+TGG + chr4_2:45911211-45911230 None:intergenic 30.0%
! TGCTGCTGATAAATGTGTTT+TGG - chr4_2:45909011-45909030 Msa0602350:three_prime_UTR 35.0%
TCTATGAGCAATGGTAAACT+TGG + chr4_2:45909064-45909083 None:intergenic 35.0%
! ACCATTGCTCATAGAGTTTT+TGG - chr4_2:45909069-45909088 Msa0602350:three_prime_UTR 35.0%
ACCAAAAACTCTATGAGCAA+TGG + chr4_2:45909073-45909092 None:intergenic 35.0%
TGATCTAAGTAAGTGGTAAG+AGG - chr4_2:45909656-45909675 Msa0602350:intron 35.0%
! ACATGATAAGGAAGCATACA+TGG - chr4_2:45909871-45909890 Msa0602350:intron 35.0%
! ACATGGTTGAGATTTTGTTG+AGG - chr4_2:45909888-45909907 Msa0602350:intron 35.0%
TGAGGTTCCAAATCATGTAA+AGG - chr4_2:45909906-45909925 Msa0602350:intron 35.0%
!!! CCCTTTTTTATTATGGTCCA+TGG - chr4_2:45909934-45909953 Msa0602350:intron 35.0%
! AGGCATTATAACGGAAAGTA+CGG + chr4_2:45909971-45909990 None:intergenic 35.0%
AAAACCAAGTGTAAACGAAG+TGG - chr4_2:45910173-45910192 Msa0602350:intron 35.0%
TCGAAAAACCGAAAGGTTTA+AGG - chr4_2:45910317-45910336 Msa0602350:intron 35.0%
GCAAAATAGAGCAACAAGAT+TGG + chr4_2:45910618-45910637 None:intergenic 35.0%
! TCTTGTTGCTCTATTTTGCA+TGG - chr4_2:45910619-45910638 Msa0602350:intron 35.0%
ATCACCACAACAATTATCAG+TGG - chr4_2:45910664-45910683 Msa0602350:intron 35.0%
TCATCCACTGATAATTGTTG+TGG + chr4_2:45910671-45910690 None:intergenic 35.0%
GATATGATGTCCATTGCTAT+GGG + chr4_2:45910711-45910730 None:intergenic 35.0%
CATAGCAATGGACATCATAT+CGG - chr4_2:45910710-45910729 Msa0602350:intron 35.0%
ATACACTTTGGACATGAACA+AGG - chr4_2:45910777-45910796 Msa0602350:intron 35.0%
! AAGTGTTTCCTTCACTGATT+TGG - chr4_2:45910850-45910869 Msa0602350:intron 35.0%
ATCGTCTGGTAAAGATTAAG+CGG - chr4_2:45910898-45910917 Msa0602350:intron 35.0%
AGCTAGGATTAATTTCCTGT+AGG - chr4_2:45911133-45911152 Msa0602350:CDS 35.0%
! TGCTGCTGATAAATGTGTTT+TGG - chr4_2:45909011-45909030 Msa0602350:three_prime_UTR 35.0%
TCTATGAGCAATGGTAAACT+TGG + chr4_2:45909064-45909083 None:intergenic 35.0%
! ACCATTGCTCATAGAGTTTT+TGG - chr4_2:45909069-45909088 Msa0602350:three_prime_UTR 35.0%
ACCAAAAACTCTATGAGCAA+TGG + chr4_2:45909073-45909092 None:intergenic 35.0%
TGATCTAAGTAAGTGGTAAG+AGG - chr4_2:45909656-45909675 Msa0602350:intron 35.0%
! ACATGATAAGGAAGCATACA+TGG - chr4_2:45909871-45909890 Msa0602350:intron 35.0%
! ACATGGTTGAGATTTTGTTG+AGG - chr4_2:45909888-45909907 Msa0602350:intron 35.0%
TGAGGTTCCAAATCATGTAA+AGG - chr4_2:45909906-45909925 Msa0602350:intron 35.0%
!!! CCCTTTTTTATTATGGTCCA+TGG - chr4_2:45909934-45909953 Msa0602350:intron 35.0%
! AGGCATTATAACGGAAAGTA+CGG + chr4_2:45909971-45909990 None:intergenic 35.0%
AAAACCAAGTGTAAACGAAG+TGG - chr4_2:45910173-45910192 Msa0602350:intron 35.0%
TCGAAAAACCGAAAGGTTTA+AGG - chr4_2:45910317-45910336 Msa0602350:intron 35.0%
GCAAAATAGAGCAACAAGAT+TGG + chr4_2:45910618-45910637 None:intergenic 35.0%
! TCTTGTTGCTCTATTTTGCA+TGG - chr4_2:45910619-45910638 Msa0602350:intron 35.0%
ATCACCACAACAATTATCAG+TGG - chr4_2:45910664-45910683 Msa0602350:intron 35.0%
TCATCCACTGATAATTGTTG+TGG + chr4_2:45910671-45910690 None:intergenic 35.0%
GATATGATGTCCATTGCTAT+GGG + chr4_2:45910711-45910730 None:intergenic 35.0%
CATAGCAATGGACATCATAT+CGG - chr4_2:45910710-45910729 Msa0602350:intron 35.0%
ATACACTTTGGACATGAACA+AGG - chr4_2:45910777-45910796 Msa0602350:intron 35.0%
! AAGTGTTTCCTTCACTGATT+TGG - chr4_2:45910850-45910869 Msa0602350:intron 35.0%
ATCGTCTGGTAAAGATTAAG+CGG - chr4_2:45910898-45910917 Msa0602350:intron 35.0%
AGCTAGGATTAATTTCCTGT+AGG - chr4_2:45911133-45911152 Msa0602350:CDS 35.0%
AAAATCTTGCAAGCAGCACA+AGG + chr4_2:45908979-45908998 None:intergenic 40.0%
GGATCAGTAGGAGGAAAATA+AGG + chr4_2:45909039-45909058 None:intergenic 40.0%
!!! GGTCCTGCCCTTTTTTATTA+TGG - chr4_2:45909927-45909946 Msa0602350:intron 40.0%
GGACCATAATAAAAAAGGGC+AGG + chr4_2:45909933-45909952 None:intergenic 40.0%
! CATTATAACGGAAAGTACGG+GGG + chr4_2:45909968-45909987 None:intergenic 40.0%
! GCATTATAACGGAAAGTACG+GGG + chr4_2:45909969-45909988 None:intergenic 40.0%
! GGCATTATAACGGAAAGTAC+GGG + chr4_2:45909970-45909989 None:intergenic 40.0%
ATGTATGACTACAAGGAGAG+AGG + chr4_2:45910051-45910070 None:intergenic 40.0%
TTAGCCACTTCGTTTACACT+TGG + chr4_2:45910180-45910199 None:intergenic 40.0%
AGTGACAAGCATGGTTTATG+AGG - chr4_2:45910460-45910479 Msa0602350:intron 40.0%
AGAGTTAGAGACCCAGTTTA+TGG - chr4_2:45910491-45910510 Msa0602350:intron 40.0%
!!! GAAGCTCATTGACTTGCTTT+TGG + chr4_2:45910541-45910560 None:intergenic 40.0%
TACAACCTCAGCTTGTGATT+TGG + chr4_2:45910576-45910595 None:intergenic 40.0%
AATTGTTGTGGTGATTCCTG+TGG + chr4_2:45910659-45910678 None:intergenic 40.0%
CGATATGATGTCCATTGCTA+TGG + chr4_2:45910712-45910731 None:intergenic 40.0%
TTTGGACATGAACAAGGACA+AGG - chr4_2:45910783-45910802 Msa0602350:intron 40.0%
CACACAAACCAAATCAGTGA+AGG + chr4_2:45910861-45910880 None:intergenic 40.0%
! AATTTGATGACCATCTCCGA+AGG - chr4_2:45911058-45911077 Msa0602350:CDS 40.0%
AAAAGTAAGGTAGCAGAGCT+AGG - chr4_2:45911117-45911136 Msa0602350:CDS 40.0%
AAAATCTTGCAAGCAGCACA+AGG + chr4_2:45908979-45908998 None:intergenic 40.0%
GGATCAGTAGGAGGAAAATA+AGG + chr4_2:45909039-45909058 None:intergenic 40.0%
!!! GGTCCTGCCCTTTTTTATTA+TGG - chr4_2:45909927-45909946 Msa0602350:intron 40.0%
GGACCATAATAAAAAAGGGC+AGG + chr4_2:45909933-45909952 None:intergenic 40.0%
! CATTATAACGGAAAGTACGG+GGG + chr4_2:45909968-45909987 None:intergenic 40.0%
! GCATTATAACGGAAAGTACG+GGG + chr4_2:45909969-45909988 None:intergenic 40.0%
! GGCATTATAACGGAAAGTAC+GGG + chr4_2:45909970-45909989 None:intergenic 40.0%
ATGTATGACTACAAGGAGAG+AGG + chr4_2:45910051-45910070 None:intergenic 40.0%
TTAGCCACTTCGTTTACACT+TGG + chr4_2:45910180-45910199 None:intergenic 40.0%
AGTGACAAGCATGGTTTATG+AGG - chr4_2:45910460-45910479 Msa0602350:intron 40.0%
AGAGTTAGAGACCCAGTTTA+TGG - chr4_2:45910491-45910510 Msa0602350:intron 40.0%
!!! GAAGCTCATTGACTTGCTTT+TGG + chr4_2:45910541-45910560 None:intergenic 40.0%
TACAACCTCAGCTTGTGATT+TGG + chr4_2:45910576-45910595 None:intergenic 40.0%
AATTGTTGTGGTGATTCCTG+TGG + chr4_2:45910659-45910678 None:intergenic 40.0%
CGATATGATGTCCATTGCTA+TGG + chr4_2:45910712-45910731 None:intergenic 40.0%
TTTGGACATGAACAAGGACA+AGG - chr4_2:45910783-45910802 Msa0602350:intron 40.0%
CACACAAACCAAATCAGTGA+AGG + chr4_2:45910861-45910880 None:intergenic 40.0%
! AATTTGATGACCATCTCCGA+AGG - chr4_2:45911058-45911077 Msa0602350:CDS 40.0%
AAAAGTAAGGTAGCAGAGCT+AGG - chr4_2:45911117-45911136 Msa0602350:CDS 40.0%
!!! GTGCTGCTTGCAAGATTTTG+AGG - chr4_2:45908980-45908999 Msa0602350:three_prime_UTR 45.0%
GTAAACTTGGCAGGATCAGT+AGG + chr4_2:45909051-45909070 None:intergenic 45.0%
TGAGCAATGGTAAACTTGGC+AGG + chr4_2:45909060-45909079 None:intergenic 45.0%
GGCAGGACCTTTACATGATT+TGG + chr4_2:45909916-45909935 None:intergenic 45.0%
! CTTGACAGCGACTGTTGATT+AGG - chr4_2:45910247-45910266 Msa0602350:intron 45.0%
CTCCTGCACAACCATAAACT+GGG + chr4_2:45910505-45910524 None:intergenic 45.0%
ACTAGCCAAATCACAAGCTG+AGG - chr4_2:45910568-45910587 Msa0602350:intron 45.0%
GGAAATGTCACAAAGTCCAC+AGG - chr4_2:45910640-45910659 Msa0602350:intron 45.0%
CAAAGTCCTTCCCATAGCAA+TGG - chr4_2:45910698-45910717 Msa0602350:intron 45.0%
TGATGTCCATTGCTATGGGA+AGG + chr4_2:45910707-45910726 None:intergenic 45.0%
! ACAACACTAGCCTATGCTCA+GGG - chr4_2:45910750-45910769 Msa0602350:intron 45.0%
!! CTGATTTGGTTTGTGTGCAC+CGG - chr4_2:45910864-45910883 Msa0602350:intron 45.0%
TTCCATTTCTCCTTCGGAGA+TGG + chr4_2:45911071-45911090 None:intergenic 45.0%
!!! GTGCTGCTTGCAAGATTTTG+AGG - chr4_2:45908980-45908999 Msa0602350:three_prime_UTR 45.0%
GTAAACTTGGCAGGATCAGT+AGG + chr4_2:45909051-45909070 None:intergenic 45.0%
TGAGCAATGGTAAACTTGGC+AGG + chr4_2:45909060-45909079 None:intergenic 45.0%
GGCAGGACCTTTACATGATT+TGG + chr4_2:45909916-45909935 None:intergenic 45.0%
! CTTGACAGCGACTGTTGATT+AGG - chr4_2:45910247-45910266 Msa0602350:intron 45.0%
CTCCTGCACAACCATAAACT+GGG + chr4_2:45910505-45910524 None:intergenic 45.0%
ACTAGCCAAATCACAAGCTG+AGG - chr4_2:45910568-45910587 Msa0602350:intron 45.0%
GGAAATGTCACAAAGTCCAC+AGG - chr4_2:45910640-45910659 Msa0602350:intron 45.0%
CAAAGTCCTTCCCATAGCAA+TGG - chr4_2:45910698-45910717 Msa0602350:intron 45.0%
TGATGTCCATTGCTATGGGA+AGG + chr4_2:45910707-45910726 None:intergenic 45.0%
! ACAACACTAGCCTATGCTCA+GGG - chr4_2:45910750-45910769 Msa0602350:intron 45.0%
!! CTGATTTGGTTTGTGTGCAC+CGG - chr4_2:45910864-45910883 Msa0602350:intron 45.0%
TTCCATTTCTCCTTCGGAGA+TGG + chr4_2:45911071-45911090 None:intergenic 45.0%
AACTTGGCAGGATCAGTAGG+AGG + chr4_2:45909048-45909067 None:intergenic 50.0%
GCATCAGCTCTTTGAGCTTC+TGG + chr4_2:45910440-45910459 None:intergenic 50.0%
AGCTGATGCAGTGACAAGCA+TGG - chr4_2:45910451-45910470 Msa0602350:intron 50.0%
GACCCAGTTTATGGTTGTGC+AGG - chr4_2:45910500-45910519 Msa0602350:intron 50.0%
GCTCCTGCACAACCATAAAC+TGG + chr4_2:45910506-45910525 None:intergenic 50.0%
!! GAGCAACAAGATTGGCTTGC+TGG + chr4_2:45910610-45910629 None:intergenic 50.0%
! GACAACACTAGCCTATGCTC+AGG - chr4_2:45910749-45910768 Msa0602350:intron 50.0%
GACCATCTCCGAAGGAGAAA+TGG - chr4_2:45911066-45911085 Msa0602350:CDS 50.0%
AACTTGGCAGGATCAGTAGG+AGG + chr4_2:45909048-45909067 None:intergenic 50.0%
GCATCAGCTCTTTGAGCTTC+TGG + chr4_2:45910440-45910459 None:intergenic 50.0%
AGCTGATGCAGTGACAAGCA+TGG - chr4_2:45910451-45910470 Msa0602350:intron 50.0%
GACCCAGTTTATGGTTGTGC+AGG - chr4_2:45910500-45910519 Msa0602350:intron 50.0%
GCTCCTGCACAACCATAAAC+TGG + chr4_2:45910506-45910525 None:intergenic 50.0%
!! GAGCAACAAGATTGGCTTGC+TGG + chr4_2:45910610-45910629 None:intergenic 50.0%
! GACAACACTAGCCTATGCTC+AGG - chr4_2:45910749-45910768 Msa0602350:intron 50.0%
GACCATCTCCGAAGGAGAAA+TGG - chr4_2:45911066-45911085 Msa0602350:CDS 50.0%
AAGGAGAGAGGCCCACCATT+AGG + chr4_2:45910039-45910058 None:intergenic 55.0%
! ACACTAGCCTATGCTCAGGG+TGG - chr4_2:45910753-45910772 Msa0602350:intron 55.0%
! CACTAGCCTATGCTCAGGGT+GGG - chr4_2:45910754-45910773 Msa0602350:intron 55.0%
GTGTATCCCACCCTGAGCAT+AGG + chr4_2:45910763-45910782 None:intergenic 55.0%
GCTCAGGGTGGGATACACTT+TGG - chr4_2:45910765-45910784 Msa0602350:intron 55.0%
! GTTTGTGTGCACCGGCAATC+TGG - chr4_2:45910872-45910891 Msa0602350:intron 55.0%
CAGACGATGTGCCAGATTGC+CGG + chr4_2:45910886-45910905 None:intergenic 55.0%
CACGCAGTCAGTCTCATCGA+TGG + chr4_2:45911007-45911026 None:intergenic 55.0%
AAGGAGAGAGGCCCACCATT+AGG + chr4_2:45910039-45910058 None:intergenic 55.0%
! ACACTAGCCTATGCTCAGGG+TGG - chr4_2:45910753-45910772 Msa0602350:intron 55.0%
! CACTAGCCTATGCTCAGGGT+GGG - chr4_2:45910754-45910773 Msa0602350:intron 55.0%
GTGTATCCCACCCTGAGCAT+AGG + chr4_2:45910763-45910782 None:intergenic 55.0%
GCTCAGGGTGGGATACACTT+TGG - chr4_2:45910765-45910784 Msa0602350:intron 55.0%
! GTTTGTGTGCACCGGCAATC+TGG - chr4_2:45910872-45910891 Msa0602350:intron 55.0%
CAGACGATGTGCCAGATTGC+CGG + chr4_2:45910886-45910905 None:intergenic 55.0%
CACGCAGTCAGTCTCATCGA+TGG + chr4_2:45911007-45911026 None:intergenic 55.0%
GTACGGGGGCTCCATATCCA+TGG + chr4_2:45909954-45909973 None:intergenic 60.0%
CGGCAATCTGGCACATCGTC+TGG - chr4_2:45910884-45910903 Msa0602350:intron 60.0%
GTACGGGGGCTCCATATCCA+TGG + chr4_2:45909954-45909973 None:intergenic 60.0%
CGGCAATCTGGCACATCGTC+TGG - chr4_2:45910884-45910903 Msa0602350:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 45908854 45911246 45908854 ID=Msa0602350;Name=Msa0602350
chr4_2 mRNA 45908854 45911246 45908854 ID=Msa0602350-mRNA-1;Parent=Msa0602350;Name=Msa0602350-mRNA-1;_AED=0.01;_eAED=0.01;_QI=71|1|1|1|1|1|2|453|185
chr4_2 exon 45908854 45909669 45908854 ID=Msa0602350-mRNA-1:exon:10336;Parent=Msa0602350-mRNA-1
chr4_2 exon 45910981 45911246 45910981 ID=Msa0602350-mRNA-1:exon:10335;Parent=Msa0602350-mRNA-1
chr4_2 five_prime_UTR 45911176 45911246 45911176 ID=Msa0602350-mRNA-1:five_prime_utr;Parent=Msa0602350-mRNA-1
chr4_2 CDS 45910981 45911175 45910981 ID=Msa0602350-mRNA-1:cds;Parent=Msa0602350-mRNA-1
chr4_2 CDS 45909307 45909669 45909307 ID=Msa0602350-mRNA-1:cds;Parent=Msa0602350-mRNA-1
chr4_2 three_prime_UTR 45908854 45909306 45908854 ID=Msa0602350-mRNA-1:three_prime_utr;Parent=Msa0602350-mRNA-1
Gene Sequence

>Msa0602350

ATGGAGAGTGCTACTAGTGACACTTTCTCTGCTCAAGCTGTTGTTATGAGTCCTTGTGCTGCTTGCAAGATTTTGAGGAGAAGATGTGCTGCTGATAAATGTGTTTTGGCACCTTATTTTCCTCCTACTGATCCTGCCAAGTTTACCATTGCTCATAGAGTTTTTGGTGCTAGCAACATCATCAAATTCTTACAGGAACTTCCAGAAGCTCAAAGAGCTGATGCAGTGACAAGCATGGTTTATGAGGCTAGTGCAAGAGTTAGAGACCCAGTTTATGGTTGTGCAGGAGCAATTTGTCAACTCCAAAAGCAAGTCAATGAGCTTCAAGCACAACTAGCCAAATCACAAGCTGAGGTTGTAAACATGAAACTCCAGCAAGCCAATCTTGTTGCTCTATTTTGCATGGAAATGTCACAAAGTCCACAGGAATCACCACAACAATTATCAGTGGATGATTTCATTCAAAGTCCTTCCCATAGCAATGGACATCATATCGGTTTGAACTTTCTTGATGACAACACTAGCCTATGCTCAGGGTGGGATACACTTTGGACATGA
Protein sequence

>Msa0602350

MESATSDTFSAQAVVMSPCAACKILRRRCAADKCVLAPYFPPTDPAKFTIAHRVFGASNIIKFLQELPEAQRADAVTSMVYEASARVRDPVYGCAGAICQLQKQVNELQAQLAKSQAEVVNMKLQQANLVALFCMEMSQSPQESPQQLSVDDFIQSPSHSNGHHIGLNFLDDNTSLCSGWDTLWT*