AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0540110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0540110 MtrunA17_Chr4g0017931 85.827 254 36 0 1 254 1 254 1.60e-161 447
Msa0540110 MtrunA17_Chr4g0017891 70.472 254 75 0 1 254 1 254 9.33e-119 339
Msa0540110 MtrunA17_Chr2g0311321 78.539 219 47 0 1 219 1 219 1.24e-108 313
Msa0540110 MtrunA17_Chr4g0036941 55.212 259 103 3 1 252 3 255 1.36e-86 258
Msa0540110 MtrunA17_Chr2g0319361 53.462 260 107 2 1 252 1 254 4.35e-80 241
Msa0540110 MtrunA17_Chr4g0050301 58.784 148 60 1 105 252 14 160 3.45e-55 174
Msa0540110 MtrunA17_Chr3g0145641 36.047 172 95 5 80 247 19 179 3.88e-27 103
Msa0540110 MtrunA17_Chr4g0018631 31.500 200 122 4 59 247 13 208 3.96e-26 101
Msa0540110 MtrunA17_Chr2g0291661 25.131 191 121 4 78 248 128 316 2.82e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0540110 AT4G35070.1 35.106 188 100 5 78 247 78 261 1.12e-22 94.0
Msa0540110 AT4G35070.2 34.574 188 101 5 78 247 23 206 2.37e-22 92.0

Find 42 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAGTTCATCAATAATA+AGG 0.345168 4_1:+21662475 Msa0540110:CDS
AGAATTTGACAAAATACTTA+AGG 0.385423 4_1:+21662879 Msa0540110:CDS
TGAAACTTGGACATGAGAAT+TGG 0.392838 4_1:-21662396 None:intergenic
TGTGGTTGTTGTAGTTCAAT+AGG 0.393104 4_1:-21662320 None:intergenic
GGTAATCATGGATTTCCTTT+CGG 0.398155 4_1:+21662239 Msa0540110:CDS
GGATTTCCTTTCGGCATCAA+TGG 0.413294 4_1:+21662248 Msa0540110:CDS
AACTTAAGATGCTTGAAACT+TGG 0.421911 4_1:-21662409 None:intergenic
TATTATGTCGGTGGAATCGC+AGG 0.448522 4_1:+21662272 Msa0540110:CDS
CTCGAAGCTTGTCCAATATG+TGG 0.453869 4_1:+21663141 Msa0540110:CDS
CCACACTCTCATGGTAATCA+TGG 0.454463 4_1:+21662227 Msa0540110:CDS
ACAAAATCAACAACAGTGGC+AGG 0.458608 4_1:+21662355 Msa0540110:CDS
TGACAAAATACTTAAGGAAA+GGG 0.461383 4_1:+21662885 Msa0540110:CDS
TGCAAAGAAAACACAGGAGT+TGG 0.462584 4_1:+21662822 Msa0540110:CDS
TGTTGTAGTTCAATAGGTTG+TGG 0.475374 4_1:-21662314 None:intergenic
GGCATCAATGGTTATTATGT+CGG 0.476149 4_1:+21662260 Msa0540110:CDS
TTGGACATGAGAATTGGAAA+AGG 0.476885 4_1:-21662390 None:intergenic
AATAACTTTGCATAACAAGT+TGG 0.490355 4_1:+21662918 Msa0540110:CDS
CACGAAGAAAGATGCATACA+TGG 0.512767 4_1:-21663099 None:intergenic
TTGACAAAATACTTAAGGAA+AGG 0.515941 4_1:+21662884 Msa0540110:CDS
TTTCTCAAATAGAGAATCAA+AGG 0.518811 4_1:-21662438 None:intergenic
GCTGCTACAACAACATCAAA+GGG 0.527548 4_1:+21662741 Msa0540110:CDS
TGGACATGAGAATTGGAAAA+GGG 0.534112 4_1:-21662389 None:intergenic
TGCTGCTACAACAACATCAA+AGG 0.534305 4_1:+21662740 Msa0540110:CDS
GCTTACTTCTTCGCTTTCAC+CGG 0.535160 4_1:-21662995 None:intergenic
ACAAATGATGATAATAAAGA+AGG 0.542335 4_1:+21662783 Msa0540110:CDS
TTTGCATAACAAGTTGGAAG+AGG 0.544284 4_1:+21662924 Msa0540110:CDS
AAGTGTAACACAAAATCCTC+AGG 0.567006 4_1:+21663063 Msa0540110:CDS
AATGATGCAAAATCATCTAC+CGG 0.567540 4_1:+21662976 Msa0540110:CDS
AAATCTCGTAAGAAGAATTG+AGG 0.584045 4_1:+21662846 Msa0540110:CDS
ATCATGCAACCACACTCTCA+TGG 0.584855 4_1:+21662218 None:intergenic
TCAAATAGAGAATCAAAGGT+AGG 0.589676 4_1:-21662434 None:intergenic
TCTATTTGAGAAACAAGTCA+AGG 0.602819 4_1:+21662448 Msa0540110:CDS
TAATAACCATTGATGCCGAA+AGG 0.608678 4_1:-21662254 None:intergenic
CTTGAAAAGCATGTCAGACG+TGG 0.625187 4_1:+21663018 Msa0540110:CDS
ATCAATGGTTATTATGTCGG+TGG 0.625897 4_1:+21662263 Msa0540110:CDS
CAAAATCAACAACAGTGGCA+GGG 0.642850 4_1:+21662356 Msa0540110:CDS
AAAAGTTGCAAAGAAAACAC+AGG 0.671968 4_1:+21662816 Msa0540110:CDS
ATTATGTCGGTGGAATCGCA+GGG 0.686770 4_1:+21662273 Msa0540110:CDS
AATTGGAAAAGGGAACAACA+TGG 0.691856 4_1:-21662379 None:intergenic
CCATGATTACCATGAGAGTG+TGG 0.696444 4_1:-21662227 None:intergenic
AACCACAAAATCAACAACAG+TGG 0.702825 4_1:+21662351 Msa0540110:CDS
TGGCATAAATAAGACACCTG+AGG 0.722061 4_1:-21663079 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCAGTTCATCAATAATA+AGG + chr4_1:21662475-21662494 Msa0540110:CDS 20.0%
!!! TATCAATTCATACATCTTTT+AGG + chr4_1:21662625-21662644 Msa0540110:intron 20.0%
!! ACAAATGATGATAATAAAGA+AGG + chr4_1:21662783-21662802 Msa0540110:CDS 20.0%
!! AGAATTTGACAAAATACTTA+AGG + chr4_1:21662879-21662898 Msa0540110:CDS 20.0%
!! AAATCAGTTCATCAATAATA+AGG + chr4_1:21662475-21662494 Msa0540110:CDS 20.0%
!!! TATCAATTCATACATCTTTT+AGG + chr4_1:21662625-21662644 Msa0540110:intron 20.0%
!! ACAAATGATGATAATAAAGA+AGG + chr4_1:21662783-21662802 Msa0540110:CDS 20.0%
!! AGAATTTGACAAAATACTTA+AGG + chr4_1:21662879-21662898 Msa0540110:CDS 20.0%
! TTTCTCAAATAGAGAATCAA+AGG - chr4_1:21662441-21662460 None:intergenic 25.0%
! TTGACAAAATACTTAAGGAA+AGG + chr4_1:21662884-21662903 Msa0540110:CDS 25.0%
! TGACAAAATACTTAAGGAAA+GGG + chr4_1:21662885-21662904 Msa0540110:CDS 25.0%
! AATAACTTTGCATAACAAGT+TGG + chr4_1:21662918-21662937 Msa0540110:CDS 25.0%
!!! TTCTTTTTCATTCCACATAT+TGG - chr4_1:21663156-21663175 None:intergenic 25.0%
! AATATGTGGAATGAAAAAGA+AGG + chr4_1:21663155-21663174 Msa0540110:CDS 25.0%
! ATATGTGGAATGAAAAAGAA+GGG + chr4_1:21663156-21663175 Msa0540110:CDS 25.0%
! TTTCTCAAATAGAGAATCAA+AGG - chr4_1:21662441-21662460 None:intergenic 25.0%
! TTGACAAAATACTTAAGGAA+AGG + chr4_1:21662884-21662903 Msa0540110:CDS 25.0%
! TGACAAAATACTTAAGGAAA+GGG + chr4_1:21662885-21662904 Msa0540110:CDS 25.0%
! AATAACTTTGCATAACAAGT+TGG + chr4_1:21662918-21662937 Msa0540110:CDS 25.0%
!!! TTCTTTTTCATTCCACATAT+TGG - chr4_1:21663156-21663175 None:intergenic 25.0%
! AATATGTGGAATGAAAAAGA+AGG + chr4_1:21663155-21663174 Msa0540110:CDS 25.0%
! ATATGTGGAATGAAAAAGAA+GGG + chr4_1:21663156-21663175 Msa0540110:CDS 25.0%
!!! TTTTGTGGTTGATGATATTG+TGG - chr4_1:21662341-21662360 None:intergenic 30.0%
AACTTAAGATGCTTGAAACT+TGG - chr4_1:21662412-21662431 None:intergenic 30.0%
TCAAATAGAGAATCAAAGGT+AGG - chr4_1:21662437-21662456 None:intergenic 30.0%
TCTATTTGAGAAACAAGTCA+AGG + chr4_1:21662448-21662467 Msa0540110:CDS 30.0%
AAAAGTTGCAAAGAAAACAC+AGG + chr4_1:21662816-21662835 Msa0540110:CDS 30.0%
AAATCTCGTAAGAAGAATTG+AGG + chr4_1:21662846-21662865 Msa0540110:CDS 30.0%
AATGATGCAAAATCATCTAC+CGG + chr4_1:21662976-21662995 Msa0540110:CDS 30.0%
!!! TTTTGTGGTTGATGATATTG+TGG - chr4_1:21662341-21662360 None:intergenic 30.0%
AACTTAAGATGCTTGAAACT+TGG - chr4_1:21662412-21662431 None:intergenic 30.0%
TCAAATAGAGAATCAAAGGT+AGG - chr4_1:21662437-21662456 None:intergenic 30.0%
TCTATTTGAGAAACAAGTCA+AGG + chr4_1:21662448-21662467 Msa0540110:CDS 30.0%
AAAAGTTGCAAAGAAAACAC+AGG + chr4_1:21662816-21662835 Msa0540110:CDS 30.0%
AAATCTCGTAAGAAGAATTG+AGG + chr4_1:21662846-21662865 Msa0540110:CDS 30.0%
AATGATGCAAAATCATCTAC+CGG + chr4_1:21662976-21662995 Msa0540110:CDS 30.0%
GGTAATCATGGATTTCCTTT+CGG + chr4_1:21662239-21662258 Msa0540110:CDS 35.0%
TAATAACCATTGATGCCGAA+AGG - chr4_1:21662257-21662276 None:intergenic 35.0%
GGCATCAATGGTTATTATGT+CGG + chr4_1:21662260-21662279 Msa0540110:CDS 35.0%
ATCAATGGTTATTATGTCGG+TGG + chr4_1:21662263-21662282 Msa0540110:CDS 35.0%
TGTTGTAGTTCAATAGGTTG+TGG - chr4_1:21662317-21662336 None:intergenic 35.0%
TGTGGTTGTTGTAGTTCAAT+AGG - chr4_1:21662323-21662342 None:intergenic 35.0%
AACCACAAAATCAACAACAG+TGG + chr4_1:21662351-21662370 Msa0540110:CDS 35.0%
AATTGGAAAAGGGAACAACA+TGG - chr4_1:21662382-21662401 None:intergenic 35.0%
TGGACATGAGAATTGGAAAA+GGG - chr4_1:21662392-21662411 None:intergenic 35.0%
TTGGACATGAGAATTGGAAA+AGG - chr4_1:21662393-21662412 None:intergenic 35.0%
TGAAACTTGGACATGAGAAT+TGG - chr4_1:21662399-21662418 None:intergenic 35.0%
! TTTGCATAACAAGTTGGAAG+AGG + chr4_1:21662924-21662943 Msa0540110:CDS 35.0%
! GAGGATTTTGTGTTACACTT+GGG - chr4_1:21663063-21663082 None:intergenic 35.0%
! TGAGGATTTTGTGTTACACT+TGG - chr4_1:21663064-21663083 None:intergenic 35.0%
AAGTGTAACACAAAATCCTC+AGG + chr4_1:21663063-21663082 Msa0540110:CDS 35.0%
GGTAATCATGGATTTCCTTT+CGG + chr4_1:21662239-21662258 Msa0540110:CDS 35.0%
TAATAACCATTGATGCCGAA+AGG - chr4_1:21662257-21662276 None:intergenic 35.0%
GGCATCAATGGTTATTATGT+CGG + chr4_1:21662260-21662279 Msa0540110:CDS 35.0%
ATCAATGGTTATTATGTCGG+TGG + chr4_1:21662263-21662282 Msa0540110:CDS 35.0%
TGTTGTAGTTCAATAGGTTG+TGG - chr4_1:21662317-21662336 None:intergenic 35.0%
TGTGGTTGTTGTAGTTCAAT+AGG - chr4_1:21662323-21662342 None:intergenic 35.0%
AACCACAAAATCAACAACAG+TGG + chr4_1:21662351-21662370 Msa0540110:CDS 35.0%
AATTGGAAAAGGGAACAACA+TGG - chr4_1:21662382-21662401 None:intergenic 35.0%
TGGACATGAGAATTGGAAAA+GGG - chr4_1:21662392-21662411 None:intergenic 35.0%
TTGGACATGAGAATTGGAAA+AGG - chr4_1:21662393-21662412 None:intergenic 35.0%
TGAAACTTGGACATGAGAAT+TGG - chr4_1:21662399-21662418 None:intergenic 35.0%
! TTTGCATAACAAGTTGGAAG+AGG + chr4_1:21662924-21662943 Msa0540110:CDS 35.0%
! GAGGATTTTGTGTTACACTT+GGG - chr4_1:21663063-21663082 None:intergenic 35.0%
! TGAGGATTTTGTGTTACACT+TGG - chr4_1:21663064-21663083 None:intergenic 35.0%
AAGTGTAACACAAAATCCTC+AGG + chr4_1:21663063-21663082 Msa0540110:CDS 35.0%
!!! TGCCACTGTTGTTGATTTTG+TGG - chr4_1:21662356-21662375 None:intergenic 40.0%
ACAAAATCAACAACAGTGGC+AGG + chr4_1:21662355-21662374 Msa0540110:CDS 40.0%
CAAAATCAACAACAGTGGCA+GGG + chr4_1:21662356-21662375 Msa0540110:CDS 40.0%
TCTCCCTGTGATAGAATCTT+TGG + chr4_1:21662597-21662616 Msa0540110:intron 40.0%
CTCCCTGTGATAGAATCTTT+GGG + chr4_1:21662598-21662617 Msa0540110:intron 40.0%
CTCCCAAAGATTCTATCACA+GGG - chr4_1:21662603-21662622 None:intergenic 40.0%
TGCTGCTACAACAACATCAA+AGG + chr4_1:21662740-21662759 Msa0540110:CDS 40.0%
GCTGCTACAACAACATCAAA+GGG + chr4_1:21662741-21662760 Msa0540110:CDS 40.0%
TGCAAAGAAAACACAGGAGT+TGG + chr4_1:21662822-21662841 Msa0540110:CDS 40.0%
TGGCATAAATAAGACACCTG+AGG - chr4_1:21663082-21663101 None:intergenic 40.0%
CACGAAGAAAGATGCATACA+TGG - chr4_1:21663102-21663121 None:intergenic 40.0%
!!! TGCCACTGTTGTTGATTTTG+TGG - chr4_1:21662356-21662375 None:intergenic 40.0%
ACAAAATCAACAACAGTGGC+AGG + chr4_1:21662355-21662374 Msa0540110:CDS 40.0%
CAAAATCAACAACAGTGGCA+GGG + chr4_1:21662356-21662375 Msa0540110:CDS 40.0%
TCTCCCTGTGATAGAATCTT+TGG + chr4_1:21662597-21662616 Msa0540110:intron 40.0%
CTCCCTGTGATAGAATCTTT+GGG + chr4_1:21662598-21662617 Msa0540110:intron 40.0%
CTCCCAAAGATTCTATCACA+GGG - chr4_1:21662603-21662622 None:intergenic 40.0%
TGCTGCTACAACAACATCAA+AGG + chr4_1:21662740-21662759 Msa0540110:CDS 40.0%
GCTGCTACAACAACATCAAA+GGG + chr4_1:21662741-21662760 Msa0540110:CDS 40.0%
TGCAAAGAAAACACAGGAGT+TGG + chr4_1:21662822-21662841 Msa0540110:CDS 40.0%
TGGCATAAATAAGACACCTG+AGG - chr4_1:21663082-21663101 None:intergenic 40.0%
CACGAAGAAAGATGCATACA+TGG - chr4_1:21663102-21663121 None:intergenic 40.0%
CCATGATTACCATGAGAGTG+TGG - chr4_1:21662230-21662249 None:intergenic 45.0%
CCACACTCTCATGGTAATCA+TGG + chr4_1:21662227-21662246 Msa0540110:CDS 45.0%
GGATTTCCTTTCGGCATCAA+TGG + chr4_1:21662248-21662267 Msa0540110:CDS 45.0%
TATTATGTCGGTGGAATCGC+AGG + chr4_1:21662272-21662291 Msa0540110:CDS 45.0%
ATTATGTCGGTGGAATCGCA+GGG + chr4_1:21662273-21662292 Msa0540110:CDS 45.0%
CCTCCCAAAGATTCTATCAC+AGG - chr4_1:21662604-21662623 None:intergenic 45.0%
CCTGTGATAGAATCTTTGGG+AGG + chr4_1:21662601-21662620 Msa0540110:intron 45.0%
GCTTACTTCTTCGCTTTCAC+CGG - chr4_1:21662998-21663017 None:intergenic 45.0%
! CACGTCTGACATGCTTTTCA+AGG - chr4_1:21663020-21663039 None:intergenic 45.0%
! CTTGAAAAGCATGTCAGACG+TGG + chr4_1:21663018-21663037 Msa0540110:CDS 45.0%
CTCGAAGCTTGTCCAATATG+TGG + chr4_1:21663141-21663160 Msa0540110:CDS 45.0%
CCATGATTACCATGAGAGTG+TGG - chr4_1:21662230-21662249 None:intergenic 45.0%
CCACACTCTCATGGTAATCA+TGG + chr4_1:21662227-21662246 Msa0540110:CDS 45.0%
GGATTTCCTTTCGGCATCAA+TGG + chr4_1:21662248-21662267 Msa0540110:CDS 45.0%
TATTATGTCGGTGGAATCGC+AGG + chr4_1:21662272-21662291 Msa0540110:CDS 45.0%
ATTATGTCGGTGGAATCGCA+GGG + chr4_1:21662273-21662292 Msa0540110:CDS 45.0%
CCTCCCAAAGATTCTATCAC+AGG - chr4_1:21662604-21662623 None:intergenic 45.0%
CCTGTGATAGAATCTTTGGG+AGG + chr4_1:21662601-21662620 Msa0540110:intron 45.0%
GCTTACTTCTTCGCTTTCAC+CGG - chr4_1:21662998-21663017 None:intergenic 45.0%
! CACGTCTGACATGCTTTTCA+AGG - chr4_1:21663020-21663039 None:intergenic 45.0%
! CTTGAAAAGCATGTCAGACG+TGG + chr4_1:21663018-21663037 Msa0540110:CDS 45.0%
CTCGAAGCTTGTCCAATATG+TGG + chr4_1:21663141-21663160 Msa0540110:CDS 45.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 21662221 21663212 21662221 ID=Msa0540110;Name=Msa0540110
chr4_1 mRNA 21662221 21663212 21662221 ID=Msa0540110-mRNA-1;Parent=Msa0540110;Name=Msa0540110-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0|1|1|1|2|0|254
chr4_1 exon 21662221 21662496 21662221 ID=Msa0540110-mRNA-1:exon:6146;Parent=Msa0540110-mRNA-1
chr4_1 exon 21662724 21663212 21662724 ID=Msa0540110-mRNA-1:exon:6147;Parent=Msa0540110-mRNA-1
chr4_1 CDS 21662221 21662496 21662221 ID=Msa0540110-mRNA-1:cds;Parent=Msa0540110-mRNA-1
chr4_1 CDS 21662724 21663212 21662724 ID=Msa0540110-mRNA-1:cds;Parent=Msa0540110-mRNA-1
Gene Sequence

>Msa0540110

ATGCAACCACACTCTCATGGTAATCATGGATTTCCTTTCGGCATCAATGGTTATTATGTCGGTGGAATCGCAGGGTTACAACAATCTCAACATCCACAACCTATTGAACTACAACAACCACAATATCATCAACCACAAAATCAACAACAGTGGCAGGGCCATGTTGTTCCCTTTTCCAATTCTCATGTCCAAGTTTCAAGCATCTTAAGTTATCCTACCTTTGATTCTCTATTTGAGAAACAAGTCAAGGAGACAAATCAGTTCATCAATAATAAGAATGATAAATTGAAACTGCTGCTACAACAACATCAAAGGGAGCTACAAGTGACAGCACAACAAATGATGATAATAAAGAAGGAAGAAATTGTAAAAGTTGCAAAGAAAACACAGGAGTTGGAAAATCTCGTAAGAAGAATTGAGGCTGAGAATAAAGAATTTGACAAAATACTTAAGGAAAGGGAAGCGACGATAATAACTTTGCATAACAAGTTGGAAGAGGAGAAGAAGAAACTAAGAATGTTTGTAGAAAATGATGCAAAATCATCTACCGGTGAAAGCGAAGAAGTAAGCCTTGAAAAGCATGTCAGACGTGGAAACAATATTATGTTTTGTCCCAAGTGTAACACAAAATCCTCAGGTGTCTTATTTATGCCATGTATGCATCTTTCTTCGTGCAAAACGTGTGAAGCTTTACTCGAAGCTTGTCCAATATGTGGAATGAAAAAGAAGGGTGTTATTGAGATTCAGAATTTGATTTTAGATTAA

Protein sequence

>Msa0540110

MQPHSHGNHGFPFGINGYYVGGIAGLQQSQHPQPIELQQPQYHQPQNQQQWQGHVVPFSNSHVQVSSILSYPTFDSLFEKQVKETNQFINNKNDKLKLLLQQHQRELQVTAQQMMIIKKEEIVKVAKKTQELENLVRRIEAENKEFDKILKEREATIITLHNKLEEEKKKLRMFVENDAKSSTGESEEVSLEKHVRRGNNIMFCPKCNTKSSGVLFMPCMHLSSCKTCEALLEACPICGMKKKGVIEIQNLILD*