AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0540150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0540150 MtrunA17_Chr4g0017931 85.827 254 36 0 1 254 1 254 1.60e-161 447
Msa0540150 MtrunA17_Chr4g0017891 70.472 254 75 0 1 254 1 254 9.33e-119 339
Msa0540150 MtrunA17_Chr2g0311321 78.539 219 47 0 1 219 1 219 1.24e-108 313
Msa0540150 MtrunA17_Chr4g0036941 55.212 259 103 3 1 252 3 255 1.36e-86 258
Msa0540150 MtrunA17_Chr2g0319361 53.462 260 107 2 1 252 1 254 4.35e-80 241
Msa0540150 MtrunA17_Chr4g0050301 58.784 148 60 1 105 252 14 160 3.45e-55 174
Msa0540150 MtrunA17_Chr3g0145641 36.047 172 95 5 80 247 19 179 3.88e-27 103
Msa0540150 MtrunA17_Chr4g0018631 31.500 200 122 4 59 247 13 208 3.96e-26 101
Msa0540150 MtrunA17_Chr2g0291661 25.131 191 121 4 78 248 128 316 2.82e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0540150 AT4G35070.1 35.106 188 100 5 78 247 78 261 1.12e-22 94.0
Msa0540150 AT4G35070.2 34.574 188 101 5 78 247 23 206 2.37e-22 92.0

Find 42 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAGTTCATCAATAATA+AGG 0.345168 4_1:+21778847 Msa0540150:CDS
AGAATTTGACAAAATACTTA+AGG 0.385423 4_1:+21779251 Msa0540150:CDS
TGAAACTTGGACATGAGAAT+TGG 0.392838 4_1:-21778768 None:intergenic
TGTGGTTGTTGTAGTTCAAT+AGG 0.393104 4_1:-21778692 None:intergenic
GGTAATCATGGATTTCCTTT+CGG 0.398155 4_1:+21778611 Msa0540150:CDS
GGATTTCCTTTCGGCATCAA+TGG 0.413294 4_1:+21778620 Msa0540150:CDS
AACTTAAGATGCTTGAAACT+TGG 0.421911 4_1:-21778781 None:intergenic
TATTATGTCGGTGGAATCGC+AGG 0.448522 4_1:+21778644 Msa0540150:CDS
CTCGAAGCTTGTCCAATATG+TGG 0.453869 4_1:+21779513 Msa0540150:CDS
CCACACTCTCATGGTAATCA+TGG 0.454463 4_1:+21778599 Msa0540150:CDS
ACAAAATCAACAACAGTGGC+AGG 0.458608 4_1:+21778727 Msa0540150:CDS
TGACAAAATACTTAAGGAAA+GGG 0.461383 4_1:+21779257 Msa0540150:CDS
TGCAAAGAAAACACAGGAGT+TGG 0.462584 4_1:+21779194 Msa0540150:CDS
TGTTGTAGTTCAATAGGTTG+TGG 0.475374 4_1:-21778686 None:intergenic
GGCATCAATGGTTATTATGT+CGG 0.476149 4_1:+21778632 Msa0540150:CDS
TTGGACATGAGAATTGGAAA+AGG 0.476885 4_1:-21778762 None:intergenic
AATAACTTTGCATAACAAGT+TGG 0.490355 4_1:+21779290 Msa0540150:CDS
CACGAAGAAAGATGCATACA+TGG 0.512767 4_1:-21779471 None:intergenic
TTGACAAAATACTTAAGGAA+AGG 0.515941 4_1:+21779256 Msa0540150:CDS
TTTCTCAAATAGAGAATCAA+AGG 0.518811 4_1:-21778810 None:intergenic
GCTGCTACAACAACATCAAA+GGG 0.527548 4_1:+21779113 Msa0540150:CDS
TGGACATGAGAATTGGAAAA+GGG 0.534112 4_1:-21778761 None:intergenic
TGCTGCTACAACAACATCAA+AGG 0.534305 4_1:+21779112 Msa0540150:CDS
GCTTACTTCTTCGCTTTCAC+CGG 0.535160 4_1:-21779367 None:intergenic
ACAAATGATGATAATAAAGA+AGG 0.542335 4_1:+21779155 Msa0540150:CDS
TTTGCATAACAAGTTGGAAG+AGG 0.544284 4_1:+21779296 Msa0540150:CDS
AAGTGTAACACAAAATCCTC+AGG 0.567006 4_1:+21779435 Msa0540150:CDS
AATGATGCAAAATCATCTAC+CGG 0.567540 4_1:+21779348 Msa0540150:CDS
AAATCTCGTAAGAAGAATTG+AGG 0.584045 4_1:+21779218 Msa0540150:CDS
ATCATGCAACCACACTCTCA+TGG 0.584855 4_1:+21778590 None:intergenic
TCAAATAGAGAATCAAAGGT+AGG 0.589676 4_1:-21778806 None:intergenic
TCTATTTGAGAAACAAGTCA+AGG 0.602819 4_1:+21778820 Msa0540150:CDS
TAATAACCATTGATGCCGAA+AGG 0.608678 4_1:-21778626 None:intergenic
CTTGAAAAGCATGTCAGACG+TGG 0.625187 4_1:+21779390 Msa0540150:CDS
ATCAATGGTTATTATGTCGG+TGG 0.625897 4_1:+21778635 Msa0540150:CDS
CAAAATCAACAACAGTGGCA+GGG 0.642850 4_1:+21778728 Msa0540150:CDS
AAAAGTTGCAAAGAAAACAC+AGG 0.671968 4_1:+21779188 Msa0540150:CDS
ATTATGTCGGTGGAATCGCA+GGG 0.686770 4_1:+21778645 Msa0540150:CDS
AATTGGAAAAGGGAACAACA+TGG 0.691856 4_1:-21778751 None:intergenic
CCATGATTACCATGAGAGTG+TGG 0.696444 4_1:-21778599 None:intergenic
AACCACAAAATCAACAACAG+TGG 0.702825 4_1:+21778723 Msa0540150:CDS
TGGCATAAATAAGACACCTG+AGG 0.722061 4_1:-21779451 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCAGTTCATCAATAATA+AGG + chr4_1:21778847-21778866 Msa0540150:CDS 20.0%
!!! TATCAATTCATACATCTTTT+AGG + chr4_1:21778997-21779016 Msa0540150:intron 20.0%
!! ACAAATGATGATAATAAAGA+AGG + chr4_1:21779155-21779174 Msa0540150:CDS 20.0%
!! AGAATTTGACAAAATACTTA+AGG + chr4_1:21779251-21779270 Msa0540150:CDS 20.0%
!! AAATCAGTTCATCAATAATA+AGG + chr4_1:21778847-21778866 Msa0540150:CDS 20.0%
!!! TATCAATTCATACATCTTTT+AGG + chr4_1:21778997-21779016 Msa0540150:intron 20.0%
!! ACAAATGATGATAATAAAGA+AGG + chr4_1:21779155-21779174 Msa0540150:CDS 20.0%
!! AGAATTTGACAAAATACTTA+AGG + chr4_1:21779251-21779270 Msa0540150:CDS 20.0%
! TTTCTCAAATAGAGAATCAA+AGG - chr4_1:21778813-21778832 None:intergenic 25.0%
! TTGACAAAATACTTAAGGAA+AGG + chr4_1:21779256-21779275 Msa0540150:CDS 25.0%
! TGACAAAATACTTAAGGAAA+GGG + chr4_1:21779257-21779276 Msa0540150:CDS 25.0%
! AATAACTTTGCATAACAAGT+TGG + chr4_1:21779290-21779309 Msa0540150:CDS 25.0%
!!! TTCTTTTTCATTCCACATAT+TGG - chr4_1:21779528-21779547 None:intergenic 25.0%
! AATATGTGGAATGAAAAAGA+AGG + chr4_1:21779527-21779546 Msa0540150:CDS 25.0%
! ATATGTGGAATGAAAAAGAA+GGG + chr4_1:21779528-21779547 Msa0540150:CDS 25.0%
! TTTCTCAAATAGAGAATCAA+AGG - chr4_1:21778813-21778832 None:intergenic 25.0%
! TTGACAAAATACTTAAGGAA+AGG + chr4_1:21779256-21779275 Msa0540150:CDS 25.0%
! TGACAAAATACTTAAGGAAA+GGG + chr4_1:21779257-21779276 Msa0540150:CDS 25.0%
! AATAACTTTGCATAACAAGT+TGG + chr4_1:21779290-21779309 Msa0540150:CDS 25.0%
!!! TTCTTTTTCATTCCACATAT+TGG - chr4_1:21779528-21779547 None:intergenic 25.0%
! AATATGTGGAATGAAAAAGA+AGG + chr4_1:21779527-21779546 Msa0540150:CDS 25.0%
! ATATGTGGAATGAAAAAGAA+GGG + chr4_1:21779528-21779547 Msa0540150:CDS 25.0%
!!! TTTTGTGGTTGATGATATTG+TGG - chr4_1:21778713-21778732 None:intergenic 30.0%
AACTTAAGATGCTTGAAACT+TGG - chr4_1:21778784-21778803 None:intergenic 30.0%
TCAAATAGAGAATCAAAGGT+AGG - chr4_1:21778809-21778828 None:intergenic 30.0%
TCTATTTGAGAAACAAGTCA+AGG + chr4_1:21778820-21778839 Msa0540150:CDS 30.0%
AAAAGTTGCAAAGAAAACAC+AGG + chr4_1:21779188-21779207 Msa0540150:CDS 30.0%
AAATCTCGTAAGAAGAATTG+AGG + chr4_1:21779218-21779237 Msa0540150:CDS 30.0%
AATGATGCAAAATCATCTAC+CGG + chr4_1:21779348-21779367 Msa0540150:CDS 30.0%
!!! TTTTGTGGTTGATGATATTG+TGG - chr4_1:21778713-21778732 None:intergenic 30.0%
AACTTAAGATGCTTGAAACT+TGG - chr4_1:21778784-21778803 None:intergenic 30.0%
TCAAATAGAGAATCAAAGGT+AGG - chr4_1:21778809-21778828 None:intergenic 30.0%
TCTATTTGAGAAACAAGTCA+AGG + chr4_1:21778820-21778839 Msa0540150:CDS 30.0%
AAAAGTTGCAAAGAAAACAC+AGG + chr4_1:21779188-21779207 Msa0540150:CDS 30.0%
AAATCTCGTAAGAAGAATTG+AGG + chr4_1:21779218-21779237 Msa0540150:CDS 30.0%
AATGATGCAAAATCATCTAC+CGG + chr4_1:21779348-21779367 Msa0540150:CDS 30.0%
GGTAATCATGGATTTCCTTT+CGG + chr4_1:21778611-21778630 Msa0540150:CDS 35.0%
TAATAACCATTGATGCCGAA+AGG - chr4_1:21778629-21778648 None:intergenic 35.0%
GGCATCAATGGTTATTATGT+CGG + chr4_1:21778632-21778651 Msa0540150:CDS 35.0%
ATCAATGGTTATTATGTCGG+TGG + chr4_1:21778635-21778654 Msa0540150:CDS 35.0%
TGTTGTAGTTCAATAGGTTG+TGG - chr4_1:21778689-21778708 None:intergenic 35.0%
TGTGGTTGTTGTAGTTCAAT+AGG - chr4_1:21778695-21778714 None:intergenic 35.0%
AACCACAAAATCAACAACAG+TGG + chr4_1:21778723-21778742 Msa0540150:CDS 35.0%
AATTGGAAAAGGGAACAACA+TGG - chr4_1:21778754-21778773 None:intergenic 35.0%
TGGACATGAGAATTGGAAAA+GGG - chr4_1:21778764-21778783 None:intergenic 35.0%
TTGGACATGAGAATTGGAAA+AGG - chr4_1:21778765-21778784 None:intergenic 35.0%
TGAAACTTGGACATGAGAAT+TGG - chr4_1:21778771-21778790 None:intergenic 35.0%
! TTTGCATAACAAGTTGGAAG+AGG + chr4_1:21779296-21779315 Msa0540150:CDS 35.0%
! GAGGATTTTGTGTTACACTT+GGG - chr4_1:21779435-21779454 None:intergenic 35.0%
! TGAGGATTTTGTGTTACACT+TGG - chr4_1:21779436-21779455 None:intergenic 35.0%
AAGTGTAACACAAAATCCTC+AGG + chr4_1:21779435-21779454 Msa0540150:CDS 35.0%
GGTAATCATGGATTTCCTTT+CGG + chr4_1:21778611-21778630 Msa0540150:CDS 35.0%
TAATAACCATTGATGCCGAA+AGG - chr4_1:21778629-21778648 None:intergenic 35.0%
GGCATCAATGGTTATTATGT+CGG + chr4_1:21778632-21778651 Msa0540150:CDS 35.0%
ATCAATGGTTATTATGTCGG+TGG + chr4_1:21778635-21778654 Msa0540150:CDS 35.0%
TGTTGTAGTTCAATAGGTTG+TGG - chr4_1:21778689-21778708 None:intergenic 35.0%
TGTGGTTGTTGTAGTTCAAT+AGG - chr4_1:21778695-21778714 None:intergenic 35.0%
AACCACAAAATCAACAACAG+TGG + chr4_1:21778723-21778742 Msa0540150:CDS 35.0%
AATTGGAAAAGGGAACAACA+TGG - chr4_1:21778754-21778773 None:intergenic 35.0%
TGGACATGAGAATTGGAAAA+GGG - chr4_1:21778764-21778783 None:intergenic 35.0%
TTGGACATGAGAATTGGAAA+AGG - chr4_1:21778765-21778784 None:intergenic 35.0%
TGAAACTTGGACATGAGAAT+TGG - chr4_1:21778771-21778790 None:intergenic 35.0%
! TTTGCATAACAAGTTGGAAG+AGG + chr4_1:21779296-21779315 Msa0540150:CDS 35.0%
! GAGGATTTTGTGTTACACTT+GGG - chr4_1:21779435-21779454 None:intergenic 35.0%
! TGAGGATTTTGTGTTACACT+TGG - chr4_1:21779436-21779455 None:intergenic 35.0%
AAGTGTAACACAAAATCCTC+AGG + chr4_1:21779435-21779454 Msa0540150:CDS 35.0%
!!! TGCCACTGTTGTTGATTTTG+TGG - chr4_1:21778728-21778747 None:intergenic 40.0%
ACAAAATCAACAACAGTGGC+AGG + chr4_1:21778727-21778746 Msa0540150:CDS 40.0%
CAAAATCAACAACAGTGGCA+GGG + chr4_1:21778728-21778747 Msa0540150:CDS 40.0%
TCTCCCTGTGATAGAATCTT+TGG + chr4_1:21778969-21778988 Msa0540150:intron 40.0%
CTCCCTGTGATAGAATCTTT+GGG + chr4_1:21778970-21778989 Msa0540150:intron 40.0%
CTCCCAAAGATTCTATCACA+GGG - chr4_1:21778975-21778994 None:intergenic 40.0%
TGCTGCTACAACAACATCAA+AGG + chr4_1:21779112-21779131 Msa0540150:CDS 40.0%
GCTGCTACAACAACATCAAA+GGG + chr4_1:21779113-21779132 Msa0540150:CDS 40.0%
TGCAAAGAAAACACAGGAGT+TGG + chr4_1:21779194-21779213 Msa0540150:CDS 40.0%
TGGCATAAATAAGACACCTG+AGG - chr4_1:21779454-21779473 None:intergenic 40.0%
CACGAAGAAAGATGCATACA+TGG - chr4_1:21779474-21779493 None:intergenic 40.0%
!!! TGCCACTGTTGTTGATTTTG+TGG - chr4_1:21778728-21778747 None:intergenic 40.0%
ACAAAATCAACAACAGTGGC+AGG + chr4_1:21778727-21778746 Msa0540150:CDS 40.0%
CAAAATCAACAACAGTGGCA+GGG + chr4_1:21778728-21778747 Msa0540150:CDS 40.0%
TCTCCCTGTGATAGAATCTT+TGG + chr4_1:21778969-21778988 Msa0540150:intron 40.0%
CTCCCTGTGATAGAATCTTT+GGG + chr4_1:21778970-21778989 Msa0540150:intron 40.0%
CTCCCAAAGATTCTATCACA+GGG - chr4_1:21778975-21778994 None:intergenic 40.0%
TGCTGCTACAACAACATCAA+AGG + chr4_1:21779112-21779131 Msa0540150:CDS 40.0%
GCTGCTACAACAACATCAAA+GGG + chr4_1:21779113-21779132 Msa0540150:CDS 40.0%
TGCAAAGAAAACACAGGAGT+TGG + chr4_1:21779194-21779213 Msa0540150:CDS 40.0%
TGGCATAAATAAGACACCTG+AGG - chr4_1:21779454-21779473 None:intergenic 40.0%
CACGAAGAAAGATGCATACA+TGG - chr4_1:21779474-21779493 None:intergenic 40.0%
CCATGATTACCATGAGAGTG+TGG - chr4_1:21778602-21778621 None:intergenic 45.0%
CCACACTCTCATGGTAATCA+TGG + chr4_1:21778599-21778618 Msa0540150:CDS 45.0%
GGATTTCCTTTCGGCATCAA+TGG + chr4_1:21778620-21778639 Msa0540150:CDS 45.0%
TATTATGTCGGTGGAATCGC+AGG + chr4_1:21778644-21778663 Msa0540150:CDS 45.0%
ATTATGTCGGTGGAATCGCA+GGG + chr4_1:21778645-21778664 Msa0540150:CDS 45.0%
CCTCCCAAAGATTCTATCAC+AGG - chr4_1:21778976-21778995 None:intergenic 45.0%
CCTGTGATAGAATCTTTGGG+AGG + chr4_1:21778973-21778992 Msa0540150:intron 45.0%
GCTTACTTCTTCGCTTTCAC+CGG - chr4_1:21779370-21779389 None:intergenic 45.0%
! CACGTCTGACATGCTTTTCA+AGG - chr4_1:21779392-21779411 None:intergenic 45.0%
! CTTGAAAAGCATGTCAGACG+TGG + chr4_1:21779390-21779409 Msa0540150:CDS 45.0%
CTCGAAGCTTGTCCAATATG+TGG + chr4_1:21779513-21779532 Msa0540150:CDS 45.0%
CCATGATTACCATGAGAGTG+TGG - chr4_1:21778602-21778621 None:intergenic 45.0%
CCACACTCTCATGGTAATCA+TGG + chr4_1:21778599-21778618 Msa0540150:CDS 45.0%
GGATTTCCTTTCGGCATCAA+TGG + chr4_1:21778620-21778639 Msa0540150:CDS 45.0%
TATTATGTCGGTGGAATCGC+AGG + chr4_1:21778644-21778663 Msa0540150:CDS 45.0%
ATTATGTCGGTGGAATCGCA+GGG + chr4_1:21778645-21778664 Msa0540150:CDS 45.0%
CCTCCCAAAGATTCTATCAC+AGG - chr4_1:21778976-21778995 None:intergenic 45.0%
CCTGTGATAGAATCTTTGGG+AGG + chr4_1:21778973-21778992 Msa0540150:intron 45.0%
GCTTACTTCTTCGCTTTCAC+CGG - chr4_1:21779370-21779389 None:intergenic 45.0%
! CACGTCTGACATGCTTTTCA+AGG - chr4_1:21779392-21779411 None:intergenic 45.0%
! CTTGAAAAGCATGTCAGACG+TGG + chr4_1:21779390-21779409 Msa0540150:CDS 45.0%
CTCGAAGCTTGTCCAATATG+TGG + chr4_1:21779513-21779532 Msa0540150:CDS 45.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 21778593 21779584 21778593 ID=Msa0540150;Name=Msa0540150
chr4_1 mRNA 21778593 21779584 21778593 ID=Msa0540150-mRNA-1;Parent=Msa0540150;Name=Msa0540150-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0|1|1|1|2|0|254
chr4_1 exon 21778593 21778868 21778593 ID=Msa0540150-mRNA-1:exon:6173;Parent=Msa0540150-mRNA-1
chr4_1 exon 21779096 21779584 21779096 ID=Msa0540150-mRNA-1:exon:6174;Parent=Msa0540150-mRNA-1
chr4_1 CDS 21778593 21778868 21778593 ID=Msa0540150-mRNA-1:cds;Parent=Msa0540150-mRNA-1
chr4_1 CDS 21779096 21779584 21779096 ID=Msa0540150-mRNA-1:cds;Parent=Msa0540150-mRNA-1
Gene Sequence

>Msa0540150

ATGCAACCACACTCTCATGGTAATCATGGATTTCCTTTCGGCATCAATGGTTATTATGTCGGTGGAATCGCAGGGTTACAACAATCTCAACATCCACAACCTATTGAACTACAACAACCACAATATCATCAACCACAAAATCAACAACAGTGGCAGGGCCATGTTGTTCCCTTTTCCAATTCTCATGTCCAAGTTTCAAGCATCTTAAGTTATCCTACCTTTGATTCTCTATTTGAGAAACAAGTCAAGGAGACAAATCAGTTCATCAATAATAAGAATGATAAATTGAAACTGCTGCTACAACAACATCAAAGGGAGCTACAAGTGACAGCACAACAAATGATGATAATAAAGAAGGAAGAAATTGTAAAAGTTGCAAAGAAAACACAGGAGTTGGAAAATCTCGTAAGAAGAATTGAGGCTGAGAATAAAGAATTTGACAAAATACTTAAGGAAAGGGAAGCGACGATAATAACTTTGCATAACAAGTTGGAAGAGGAGAAGAAGAAACTAAGAATGTTTGTAGAAAATGATGCAAAATCATCTACCGGTGAAAGCGAAGAAGTAAGCCTTGAAAAGCATGTCAGACGTGGAAACAATATTATGTTTTGTCCCAAGTGTAACACAAAATCCTCAGGTGTCTTATTTATGCCATGTATGCATCTTTCTTCGTGCAAAACGTGTGAAGCTTTACTCGAAGCTTGTCCAATATGTGGAATGAAAAAGAAGGGTGTTATTGAGATTCAGAATTTGATTTTAGATTAA

Protein sequence

>Msa0540150

MQPHSHGNHGFPFGINGYYVGGIAGLQQSQHPQPIELQQPQYHQPQNQQQWQGHVVPFSNSHVQVSSILSYPTFDSLFEKQVKETNQFINNKNDKLKLLLQQHQRELQVTAQQMMIIKKEEIVKVAKKTQELENLVRRIEAENKEFDKILKEREATIITLHNKLEEEKKKLRMFVENDAKSSTGESEEVSLEKHVRRGNNIMFCPKCNTKSSGVLFMPCMHLSSCKTCEALLEACPICGMKKKGVIEIQNLILD*