AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0674670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0674670 MtrunA17_Chr4g0017931 85.827 254 36 0 1 254 1 254 1.60e-161 447
Msa0674670 MtrunA17_Chr4g0017891 70.472 254 75 0 1 254 1 254 9.33e-119 339
Msa0674670 MtrunA17_Chr2g0311321 78.539 219 47 0 1 219 1 219 1.24e-108 313
Msa0674670 MtrunA17_Chr4g0036941 55.212 259 103 3 1 252 3 255 1.36e-86 258
Msa0674670 MtrunA17_Chr2g0319361 53.462 260 107 2 1 252 1 254 4.35e-80 241
Msa0674670 MtrunA17_Chr4g0050301 58.784 148 60 1 105 252 14 160 3.45e-55 174
Msa0674670 MtrunA17_Chr3g0145641 36.047 172 95 5 80 247 19 179 3.88e-27 103
Msa0674670 MtrunA17_Chr4g0018631 31.500 200 122 4 59 247 13 208 3.96e-26 101
Msa0674670 MtrunA17_Chr2g0291661 25.131 191 121 4 78 248 128 316 2.82e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0674670 AT4G35070.1 35.106 188 100 5 78 247 78 261 1.12e-22 94.0
Msa0674670 AT4G35070.2 34.574 188 101 5 78 247 23 206 2.37e-22 92.0

Find 42 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAGTTCATCAATAATA+AGG 0.345168 4_4:+22431870 Msa0674670:CDS
AGAATTTGACAAAATACTTA+AGG 0.385423 4_4:+22432274 Msa0674670:CDS
TGAAACTTGGACATGAGAAT+TGG 0.392838 4_4:-22431791 None:intergenic
TGTGGTTGTTGTAGTTCAAT+AGG 0.393104 4_4:-22431715 None:intergenic
GGTAATCATGGATTTCCTTT+CGG 0.398155 4_4:+22431634 Msa0674670:CDS
GGATTTCCTTTCGGCATCAA+TGG 0.413294 4_4:+22431643 Msa0674670:CDS
AACTTAAGATGCTTGAAACT+TGG 0.421911 4_4:-22431804 None:intergenic
TATTATGTCGGTGGAATCGC+AGG 0.448522 4_4:+22431667 Msa0674670:CDS
CTCGAAGCTTGTCCAATATG+TGG 0.453869 4_4:+22432536 Msa0674670:CDS
CCACACTCTCATGGTAATCA+TGG 0.454463 4_4:+22431622 Msa0674670:CDS
ACAAAATCAACAACAGTGGC+AGG 0.458608 4_4:+22431750 Msa0674670:CDS
TGACAAAATACTTAAGGAAA+GGG 0.461383 4_4:+22432280 Msa0674670:CDS
TGCAAAGAAAACACAGGAGT+TGG 0.462584 4_4:+22432217 Msa0674670:CDS
TGTTGTAGTTCAATAGGTTG+TGG 0.475374 4_4:-22431709 None:intergenic
GGCATCAATGGTTATTATGT+CGG 0.476149 4_4:+22431655 Msa0674670:CDS
TTGGACATGAGAATTGGAAA+AGG 0.476885 4_4:-22431785 None:intergenic
AATAACTTTGCATAACAAGT+TGG 0.490355 4_4:+22432313 Msa0674670:CDS
CACGAAGAAAGATGCATACA+TGG 0.512767 4_4:-22432494 None:intergenic
TTGACAAAATACTTAAGGAA+AGG 0.515941 4_4:+22432279 Msa0674670:CDS
TTTCTCAAATAGAGAATCAA+AGG 0.518811 4_4:-22431833 None:intergenic
GCTGCTACAACAACATCAAA+GGG 0.527548 4_4:+22432136 Msa0674670:CDS
TGGACATGAGAATTGGAAAA+GGG 0.534112 4_4:-22431784 None:intergenic
TGCTGCTACAACAACATCAA+AGG 0.534305 4_4:+22432135 Msa0674670:CDS
GCTTACTTCTTCGCTTTCAC+CGG 0.535160 4_4:-22432390 None:intergenic
ACAAATGATGATAATAAAGA+AGG 0.542335 4_4:+22432178 Msa0674670:CDS
TTTGCATAACAAGTTGGAAG+AGG 0.544284 4_4:+22432319 Msa0674670:CDS
AAGTGTAACACAAAATCCTC+AGG 0.567006 4_4:+22432458 Msa0674670:CDS
AATGATGCAAAATCATCTAC+CGG 0.567540 4_4:+22432371 Msa0674670:CDS
AAATCTCGTAAGAAGAATTG+AGG 0.584045 4_4:+22432241 Msa0674670:CDS
TCAAATAGAGAATCAAAGGT+AGG 0.589676 4_4:-22431829 None:intergenic
ATCATGCAACCACACTCTCA+TGG 0.599430 4_4:+22431613 None:intergenic
TCTATTTGAGAAACAAGTCA+AGG 0.602819 4_4:+22431843 Msa0674670:CDS
TAATAACCATTGATGCCGAA+AGG 0.608678 4_4:-22431649 None:intergenic
CTTGAAAAGCATGTCAGACG+TGG 0.625187 4_4:+22432413 Msa0674670:CDS
ATCAATGGTTATTATGTCGG+TGG 0.625897 4_4:+22431658 Msa0674670:CDS
CAAAATCAACAACAGTGGCA+GGG 0.642850 4_4:+22431751 Msa0674670:CDS
AAAAGTTGCAAAGAAAACAC+AGG 0.671968 4_4:+22432211 Msa0674670:CDS
ATTATGTCGGTGGAATCGCA+GGG 0.686770 4_4:+22431668 Msa0674670:CDS
AATTGGAAAAGGGAACAACA+TGG 0.691856 4_4:-22431774 None:intergenic
CCATGATTACCATGAGAGTG+TGG 0.696444 4_4:-22431622 None:intergenic
AACCACAAAATCAACAACAG+TGG 0.702825 4_4:+22431746 Msa0674670:CDS
TGGCATAAATAAGACACCTG+AGG 0.722061 4_4:-22432474 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCAGTTCATCAATAATA+AGG + chr4_4:22431870-22431889 Msa0674670:CDS 20.0%
!!! TATCAATTCATACATCTTTT+AGG + chr4_4:22432020-22432039 Msa0674670:intron 20.0%
!! ACAAATGATGATAATAAAGA+AGG + chr4_4:22432178-22432197 Msa0674670:CDS 20.0%
!! AGAATTTGACAAAATACTTA+AGG + chr4_4:22432274-22432293 Msa0674670:CDS 20.0%
!! AAATCAGTTCATCAATAATA+AGG + chr4_4:22431870-22431889 Msa0674670:CDS 20.0%
!!! TATCAATTCATACATCTTTT+AGG + chr4_4:22432020-22432039 Msa0674670:intron 20.0%
!! ACAAATGATGATAATAAAGA+AGG + chr4_4:22432178-22432197 Msa0674670:CDS 20.0%
!! AGAATTTGACAAAATACTTA+AGG + chr4_4:22432274-22432293 Msa0674670:CDS 20.0%
! TTTCTCAAATAGAGAATCAA+AGG - chr4_4:22431836-22431855 None:intergenic 25.0%
! TTGACAAAATACTTAAGGAA+AGG + chr4_4:22432279-22432298 Msa0674670:CDS 25.0%
! TGACAAAATACTTAAGGAAA+GGG + chr4_4:22432280-22432299 Msa0674670:CDS 25.0%
! AATAACTTTGCATAACAAGT+TGG + chr4_4:22432313-22432332 Msa0674670:CDS 25.0%
!!! TTCTTTTTCATTCCACATAT+TGG - chr4_4:22432551-22432570 None:intergenic 25.0%
! AATATGTGGAATGAAAAAGA+AGG + chr4_4:22432550-22432569 Msa0674670:CDS 25.0%
! ATATGTGGAATGAAAAAGAA+GGG + chr4_4:22432551-22432570 Msa0674670:CDS 25.0%
! TTTCTCAAATAGAGAATCAA+AGG - chr4_4:22431836-22431855 None:intergenic 25.0%
! TTGACAAAATACTTAAGGAA+AGG + chr4_4:22432279-22432298 Msa0674670:CDS 25.0%
! TGACAAAATACTTAAGGAAA+GGG + chr4_4:22432280-22432299 Msa0674670:CDS 25.0%
! AATAACTTTGCATAACAAGT+TGG + chr4_4:22432313-22432332 Msa0674670:CDS 25.0%
!!! TTCTTTTTCATTCCACATAT+TGG - chr4_4:22432551-22432570 None:intergenic 25.0%
! AATATGTGGAATGAAAAAGA+AGG + chr4_4:22432550-22432569 Msa0674670:CDS 25.0%
! ATATGTGGAATGAAAAAGAA+GGG + chr4_4:22432551-22432570 Msa0674670:CDS 25.0%
!!! TTTTGTGGTTGATGATATTG+TGG - chr4_4:22431736-22431755 None:intergenic 30.0%
AACTTAAGATGCTTGAAACT+TGG - chr4_4:22431807-22431826 None:intergenic 30.0%
TCAAATAGAGAATCAAAGGT+AGG - chr4_4:22431832-22431851 None:intergenic 30.0%
TCTATTTGAGAAACAAGTCA+AGG + chr4_4:22431843-22431862 Msa0674670:CDS 30.0%
AAAAGTTGCAAAGAAAACAC+AGG + chr4_4:22432211-22432230 Msa0674670:CDS 30.0%
AAATCTCGTAAGAAGAATTG+AGG + chr4_4:22432241-22432260 Msa0674670:CDS 30.0%
AATGATGCAAAATCATCTAC+CGG + chr4_4:22432371-22432390 Msa0674670:CDS 30.0%
!!! TTTTGTGGTTGATGATATTG+TGG - chr4_4:22431736-22431755 None:intergenic 30.0%
AACTTAAGATGCTTGAAACT+TGG - chr4_4:22431807-22431826 None:intergenic 30.0%
TCAAATAGAGAATCAAAGGT+AGG - chr4_4:22431832-22431851 None:intergenic 30.0%
TCTATTTGAGAAACAAGTCA+AGG + chr4_4:22431843-22431862 Msa0674670:CDS 30.0%
AAAAGTTGCAAAGAAAACAC+AGG + chr4_4:22432211-22432230 Msa0674670:CDS 30.0%
AAATCTCGTAAGAAGAATTG+AGG + chr4_4:22432241-22432260 Msa0674670:CDS 30.0%
AATGATGCAAAATCATCTAC+CGG + chr4_4:22432371-22432390 Msa0674670:CDS 30.0%
GGTAATCATGGATTTCCTTT+CGG + chr4_4:22431634-22431653 Msa0674670:CDS 35.0%
TAATAACCATTGATGCCGAA+AGG - chr4_4:22431652-22431671 None:intergenic 35.0%
GGCATCAATGGTTATTATGT+CGG + chr4_4:22431655-22431674 Msa0674670:CDS 35.0%
ATCAATGGTTATTATGTCGG+TGG + chr4_4:22431658-22431677 Msa0674670:CDS 35.0%
TGTTGTAGTTCAATAGGTTG+TGG - chr4_4:22431712-22431731 None:intergenic 35.0%
TGTGGTTGTTGTAGTTCAAT+AGG - chr4_4:22431718-22431737 None:intergenic 35.0%
AACCACAAAATCAACAACAG+TGG + chr4_4:22431746-22431765 Msa0674670:CDS 35.0%
AATTGGAAAAGGGAACAACA+TGG - chr4_4:22431777-22431796 None:intergenic 35.0%
TGGACATGAGAATTGGAAAA+GGG - chr4_4:22431787-22431806 None:intergenic 35.0%
TTGGACATGAGAATTGGAAA+AGG - chr4_4:22431788-22431807 None:intergenic 35.0%
TGAAACTTGGACATGAGAAT+TGG - chr4_4:22431794-22431813 None:intergenic 35.0%
! TTTGCATAACAAGTTGGAAG+AGG + chr4_4:22432319-22432338 Msa0674670:CDS 35.0%
! GAGGATTTTGTGTTACACTT+GGG - chr4_4:22432458-22432477 None:intergenic 35.0%
! TGAGGATTTTGTGTTACACT+TGG - chr4_4:22432459-22432478 None:intergenic 35.0%
AAGTGTAACACAAAATCCTC+AGG + chr4_4:22432458-22432477 Msa0674670:CDS 35.0%
GGTAATCATGGATTTCCTTT+CGG + chr4_4:22431634-22431653 Msa0674670:CDS 35.0%
TAATAACCATTGATGCCGAA+AGG - chr4_4:22431652-22431671 None:intergenic 35.0%
GGCATCAATGGTTATTATGT+CGG + chr4_4:22431655-22431674 Msa0674670:CDS 35.0%
ATCAATGGTTATTATGTCGG+TGG + chr4_4:22431658-22431677 Msa0674670:CDS 35.0%
TGTTGTAGTTCAATAGGTTG+TGG - chr4_4:22431712-22431731 None:intergenic 35.0%
TGTGGTTGTTGTAGTTCAAT+AGG - chr4_4:22431718-22431737 None:intergenic 35.0%
AACCACAAAATCAACAACAG+TGG + chr4_4:22431746-22431765 Msa0674670:CDS 35.0%
AATTGGAAAAGGGAACAACA+TGG - chr4_4:22431777-22431796 None:intergenic 35.0%
TGGACATGAGAATTGGAAAA+GGG - chr4_4:22431787-22431806 None:intergenic 35.0%
TTGGACATGAGAATTGGAAA+AGG - chr4_4:22431788-22431807 None:intergenic 35.0%
TGAAACTTGGACATGAGAAT+TGG - chr4_4:22431794-22431813 None:intergenic 35.0%
! TTTGCATAACAAGTTGGAAG+AGG + chr4_4:22432319-22432338 Msa0674670:CDS 35.0%
! GAGGATTTTGTGTTACACTT+GGG - chr4_4:22432458-22432477 None:intergenic 35.0%
! TGAGGATTTTGTGTTACACT+TGG - chr4_4:22432459-22432478 None:intergenic 35.0%
AAGTGTAACACAAAATCCTC+AGG + chr4_4:22432458-22432477 Msa0674670:CDS 35.0%
!!! TGCCACTGTTGTTGATTTTG+TGG - chr4_4:22431751-22431770 None:intergenic 40.0%
ACAAAATCAACAACAGTGGC+AGG + chr4_4:22431750-22431769 Msa0674670:CDS 40.0%
CAAAATCAACAACAGTGGCA+GGG + chr4_4:22431751-22431770 Msa0674670:CDS 40.0%
TCTCCCTGTGATAGAATCTT+TGG + chr4_4:22431992-22432011 Msa0674670:intron 40.0%
CTCCCTGTGATAGAATCTTT+GGG + chr4_4:22431993-22432012 Msa0674670:intron 40.0%
CTCCCAAAGATTCTATCACA+GGG - chr4_4:22431998-22432017 None:intergenic 40.0%
TGCTGCTACAACAACATCAA+AGG + chr4_4:22432135-22432154 Msa0674670:CDS 40.0%
GCTGCTACAACAACATCAAA+GGG + chr4_4:22432136-22432155 Msa0674670:CDS 40.0%
TGCAAAGAAAACACAGGAGT+TGG + chr4_4:22432217-22432236 Msa0674670:CDS 40.0%
TGGCATAAATAAGACACCTG+AGG - chr4_4:22432477-22432496 None:intergenic 40.0%
CACGAAGAAAGATGCATACA+TGG - chr4_4:22432497-22432516 None:intergenic 40.0%
!!! TGCCACTGTTGTTGATTTTG+TGG - chr4_4:22431751-22431770 None:intergenic 40.0%
ACAAAATCAACAACAGTGGC+AGG + chr4_4:22431750-22431769 Msa0674670:CDS 40.0%
CAAAATCAACAACAGTGGCA+GGG + chr4_4:22431751-22431770 Msa0674670:CDS 40.0%
TCTCCCTGTGATAGAATCTT+TGG + chr4_4:22431992-22432011 Msa0674670:intron 40.0%
CTCCCTGTGATAGAATCTTT+GGG + chr4_4:22431993-22432012 Msa0674670:intron 40.0%
CTCCCAAAGATTCTATCACA+GGG - chr4_4:22431998-22432017 None:intergenic 40.0%
TGCTGCTACAACAACATCAA+AGG + chr4_4:22432135-22432154 Msa0674670:CDS 40.0%
GCTGCTACAACAACATCAAA+GGG + chr4_4:22432136-22432155 Msa0674670:CDS 40.0%
TGCAAAGAAAACACAGGAGT+TGG + chr4_4:22432217-22432236 Msa0674670:CDS 40.0%
TGGCATAAATAAGACACCTG+AGG - chr4_4:22432477-22432496 None:intergenic 40.0%
CACGAAGAAAGATGCATACA+TGG - chr4_4:22432497-22432516 None:intergenic 40.0%
CCATGATTACCATGAGAGTG+TGG - chr4_4:22431625-22431644 None:intergenic 45.0%
CCACACTCTCATGGTAATCA+TGG + chr4_4:22431622-22431641 Msa0674670:CDS 45.0%
GGATTTCCTTTCGGCATCAA+TGG + chr4_4:22431643-22431662 Msa0674670:CDS 45.0%
TATTATGTCGGTGGAATCGC+AGG + chr4_4:22431667-22431686 Msa0674670:CDS 45.0%
ATTATGTCGGTGGAATCGCA+GGG + chr4_4:22431668-22431687 Msa0674670:CDS 45.0%
CCTCCCAAAGATTCTATCAC+AGG - chr4_4:22431999-22432018 None:intergenic 45.0%
CCTGTGATAGAATCTTTGGG+AGG + chr4_4:22431996-22432015 Msa0674670:intron 45.0%
GCTTACTTCTTCGCTTTCAC+CGG - chr4_4:22432393-22432412 None:intergenic 45.0%
! CACGTCTGACATGCTTTTCA+AGG - chr4_4:22432415-22432434 None:intergenic 45.0%
! CTTGAAAAGCATGTCAGACG+TGG + chr4_4:22432413-22432432 Msa0674670:CDS 45.0%
CTCGAAGCTTGTCCAATATG+TGG + chr4_4:22432536-22432555 Msa0674670:CDS 45.0%
CCATGATTACCATGAGAGTG+TGG - chr4_4:22431625-22431644 None:intergenic 45.0%
CCACACTCTCATGGTAATCA+TGG + chr4_4:22431622-22431641 Msa0674670:CDS 45.0%
GGATTTCCTTTCGGCATCAA+TGG + chr4_4:22431643-22431662 Msa0674670:CDS 45.0%
TATTATGTCGGTGGAATCGC+AGG + chr4_4:22431667-22431686 Msa0674670:CDS 45.0%
ATTATGTCGGTGGAATCGCA+GGG + chr4_4:22431668-22431687 Msa0674670:CDS 45.0%
CCTCCCAAAGATTCTATCAC+AGG - chr4_4:22431999-22432018 None:intergenic 45.0%
CCTGTGATAGAATCTTTGGG+AGG + chr4_4:22431996-22432015 Msa0674670:intron 45.0%
GCTTACTTCTTCGCTTTCAC+CGG - chr4_4:22432393-22432412 None:intergenic 45.0%
! CACGTCTGACATGCTTTTCA+AGG - chr4_4:22432415-22432434 None:intergenic 45.0%
! CTTGAAAAGCATGTCAGACG+TGG + chr4_4:22432413-22432432 Msa0674670:CDS 45.0%
CTCGAAGCTTGTCCAATATG+TGG + chr4_4:22432536-22432555 Msa0674670:CDS 45.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 22431616 22432607 22431616 ID=Msa0674670;Name=Msa0674670
chr4_4 mRNA 22431616 22432607 22431616 ID=Msa0674670-mRNA-1;Parent=Msa0674670;Name=Msa0674670-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0|1|1|1|2|0|254
chr4_4 exon 22431616 22431891 22431616 ID=Msa0674670-mRNA-1:exon:6311;Parent=Msa0674670-mRNA-1
chr4_4 exon 22432119 22432607 22432119 ID=Msa0674670-mRNA-1:exon:6312;Parent=Msa0674670-mRNA-1
chr4_4 CDS 22431616 22431891 22431616 ID=Msa0674670-mRNA-1:cds;Parent=Msa0674670-mRNA-1
chr4_4 CDS 22432119 22432607 22432119 ID=Msa0674670-mRNA-1:cds;Parent=Msa0674670-mRNA-1
Gene Sequence

>Msa0674670

ATGCAACCACACTCTCATGGTAATCATGGATTTCCTTTCGGCATCAATGGTTATTATGTCGGTGGAATCGCAGGGTTACAACAATCTCAACATCCACAACCTATTGAACTACAACAACCACAATATCATCAACCACAAAATCAACAACAGTGGCAGGGCCATGTTGTTCCCTTTTCCAATTCTCATGTCCAAGTTTCAAGCATCTTAAGTTATCCTACCTTTGATTCTCTATTTGAGAAACAAGTCAAGGAGACAAATCAGTTCATCAATAATAAGAATGATAAATTGAAACTGCTGCTACAACAACATCAAAGGGAGCTACAAGTGACAGCACAACAAATGATGATAATAAAGAAGGAAGAAATTGTAAAAGTTGCAAAGAAAACACAGGAGTTGGAAAATCTCGTAAGAAGAATTGAGGCTGAGAATAAAGAATTTGACAAAATACTTAAGGAAAGGGAAGCGACGATAATAACTTTGCATAACAAGTTGGAAGAGGAGAAGAAGAAACTAAGAATGTTTGTAGAAAATGATGCAAAATCATCTACCGGTGAAAGCGAAGAAGTAAGCCTTGAAAAGCATGTCAGACGTGGAAACAATATTATGTTTTGTCCCAAGTGTAACACAAAATCCTCAGGTGTCTTATTTATGCCATGTATGCATCTTTCTTCGTGCAAAACGTGTGAAGCTTTACTCGAAGCTTGTCCAATATGTGGAATGAAAAAGAAGGGTGTTATTGAGATTCAGAATTTGATTTTAGATTAA

Protein sequence

>Msa0674670

MQPHSHGNHGFPFGINGYYVGGIAGLQQSQHPQPIELQQPQYHQPQNQQQWQGHVVPFSNSHVQVSSILSYPTFDSLFEKQVKETNQFINNKNDKLKLLLQQHQRELQVTAQQMMIIKKEEIVKVAKKTQELENLVRRIEAENKEFDKILKEREATIITLHNKLEEEKKKLRMFVENDAKSSTGESEEVSLEKHVRRGNNIMFCPKCNTKSSGVLFMPCMHLSSCKTCEALLEACPICGMKKKGVIEIQNLILD*