AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0541380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541380 MtrunA17_Chr2g0295351 97.436 78 2 0 1 78 220 297 1.11e-44 154
Msa0541380 MtrunA17_Chr2g0295351 87.234 47 6 0 79 125 358 404 1.21e-22 92.0
Msa0541380 MtrunA17_Chr2g0295281 90.741 54 5 0 26 79 58 111 1.61e-27 102
Msa0541380 MtrunA17_Chr2g0295281 95.122 41 2 0 80 120 182 222 2.92e-21 85.9
Msa0541380 MtrunA17_Chr2g0310461 41.727 139 36 2 31 125 67 204 7.23e-25 96.7
Msa0541380 MtrunA17_Chr2g0295251 64.815 54 19 0 25 78 52 105 8.86e-17 75.1
Msa0541380 MtrunA17_Chr2g0295251 76.190 42 10 0 79 120 164 205 5.48e-16 72.8
Msa0541380 MtrunA17_Chr4g0067791 55.357 56 23 1 63 118 409 462 2.79e-13 65.1
Msa0541380 MtrunA17_Chr7g0271991 56.522 46 20 0 72 117 417 462 7.61e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 97 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCATAGTTGTTCATATT+TGG 0.190625 4_1:-25243580 Msa0541380:three_prime_UTR
CTTTGCCTCATGAAGGATTA+AGG 0.224037 4_1:-25246851 Msa0541380:five_prime_UTR
CATCAAGAGCAAAGTTGTTT+TGG 0.224965 4_1:+25243436 None:intergenic
GCTGGTACATGATTGTATTT+TGG 0.236606 4_1:-25246765 Msa0541380:five_prime_UTR
CAACAGAATTTAACAATAAA+TGG 0.239575 4_1:+25246878 None:intergenic
TTTGAAACTTCTGTAGTTTA+GGG 0.243309 4_1:-25243353 Msa0541380:three_prime_UTR
TAATATATGCAAATATTTAA+TGG 0.244366 4_1:-25243486 Msa0541380:three_prime_UTR
TGATTAATAATGCTCTAGTT+TGG 0.251519 4_1:-25247039 Msa0541380:five_prime_UTR
TTACATGTGATTATGTCTTT+TGG 0.264580 4_1:-25243514 Msa0541380:three_prime_UTR
TGGAGATGATAAGTTAATTA+AGG 0.291102 4_1:-25245201 Msa0541380:intron
AAGGAAGGATCCATATTCTA+TGG 0.291219 4_1:-25246498 Msa0541380:five_prime_UTR
GACATCTGCATTAATATTAA+AGG 0.293442 4_1:-25246517 Msa0541380:five_prime_UTR
AGGATCAACTGTAACAAATA+TGG 0.308984 4_1:-25245986 Msa0541380:CDS
AATTGAAATCCCATAGAATA+TGG 0.331847 4_1:+25246488 None:intergenic
TGGTTTCTTAAGTTTCCTTT+TGG 0.338160 4_1:-25246567 Msa0541380:five_prime_UTR
CTGAAATTGCATTATGAACT+TGG 0.342714 4_1:-25246419 Msa0541380:five_prime_UTR
GATTAATAATGCTCTAGTTT+GGG 0.344381 4_1:-25247038 Msa0541380:five_prime_UTR
AACCAATTTGAATAAGTAAA+AGG 0.354750 4_1:+25246665 None:intergenic
GGTGAGGTTCCGGTGTGTTT+TGG 0.360724 4_1:-25243559 Msa0541380:three_prime_UTR
ATAGCATGACTGCAGTTATA+AGG 0.361544 4_1:-25246539 Msa0541380:five_prime_UTR
GTTCATATTTGGTGAGGTTC+CGG 0.366406 4_1:-25243569 Msa0541380:three_prime_UTR
CCAACAATGTCTTAGAATAA+TGG 0.367295 4_1:+25246980 None:intergenic
TGTTTACAGTGTGTTTGGTT+AGG 0.370931 4_1:-25246639 Msa0541380:five_prime_UTR
CCATTATTCTAAGACATTGT+TGG 0.372999 4_1:-25246980 Msa0541380:five_prime_UTR
TAATGGCCTACAAGAGCATT+TGG 0.373553 4_1:-25245389 Msa0541380:CDS
TCAATTCATTCTATCCTTGC+TGG 0.376168 4_1:-25245952 Msa0541380:intron
ATACCTCCAAATGCTCTTGT+AGG 0.377214 4_1:+25245383 None:intergenic
ATTTCAATTTCACCTAGAAA+TGG 0.389485 4_1:-25246474 Msa0541380:five_prime_UTR
CAAAATTAAGTTATAATAAG+TGG 0.392008 4_1:+25246321 None:intergenic
TATACCCATCAAGTTCATAA+TGG 0.400762 4_1:-25245406 Msa0541380:CDS
TAAGACATTGTTGGTTGTCC+TGG 0.404402 4_1:-25246971 Msa0541380:five_prime_UTR
TTGGACAAATTACTACCCTT+TGG 0.417675 4_1:-25246746 Msa0541380:five_prime_UTR
TAATATCTTCAAACCTTGCC+AGG 0.435910 4_1:+25246953 None:intergenic
GCAGCAGGGACTGGTGGAAA+TGG 0.437312 4_1:+25246293 None:intergenic
GTAGGCCATTATGAACTTGA+TGG 0.438851 4_1:+25245401 None:intergenic
TTTGCTCTTGATGCTTAATC+CGG 0.440596 4_1:-25243426 Msa0541380:three_prime_UTR
CTCGCAGCTTCCTCATTGCC+CGG 0.447826 4_1:-25246197 Msa0541380:CDS
TTGTGTTCTTTGCCTCATGA+AGG 0.451703 4_1:-25246858 Msa0541380:five_prime_UTR
TTCAGCAGGAAGCGCAGCTA+AGG 0.454203 4_1:+25246437 None:intergenic
CAGTTCAGGCCAACCAGGTC+CGG 0.460674 4_1:+25243407 None:intergenic
ATATACCTTTACATCTCAAC+CGG 0.463240 4_1:-25246061 Msa0541380:CDS
TTTCGGTTGTTTATATACAT+TGG 0.464847 4_1:-25246356 Msa0541380:five_prime_UTR
GCACTAGGAGCAGCAGGGAC+TGG 0.470970 4_1:+25246284 None:intergenic
GCAAGGATAGAATGAATTGA+TGG 0.478088 4_1:+25245955 None:intergenic
TTCAACAGATTGTGAAGGAC+CGG 0.486254 4_1:+25246178 None:intergenic
TTAGTGACACGGGAAATTGC+TGG 0.486259 4_1:-25246783 Msa0541380:five_prime_UTR
CTCGGTTCTGCACGTGCTAG+TGG 0.487109 4_1:+25243613 None:intergenic
GTTCATAATGCAATTTCAGC+AGG 0.487930 4_1:+25246423 None:intergenic
CTTGATGCTTAATCCGGACC+TGG 0.488222 4_1:-25243420 Msa0541380:three_prime_UTR
AACATTAGTTACCATGGGAC+TGG 0.489616 4_1:-25246587 Msa0541380:five_prime_UTR
CATTGTTGGTTGTCCTGGCA+AGG 0.489748 4_1:-25246966 Msa0541380:five_prime_UTR
GCATTAGCACTAGGAGCAGC+AGG 0.491625 4_1:+25246278 None:intergenic
TAATGTACGTATGAACTAGA+TGG 0.496408 4_1:+25246926 None:intergenic
ATTGTTTGCAAGTTACATCA+AGG 0.496676 4_1:-25246006 Msa0541380:CDS
CACAAGTTACAACGCATAGC+TGG 0.501837 4_1:+25245222 None:intergenic
GGCGTGCAATGTCCATTTCT+AGG 0.509480 4_1:+25246462 None:intergenic
TGATGGTGGTGTTAATGACT+TGG 0.513959 4_1:-25245263 Msa0541380:CDS
AACTTGCAAACAATAGTTGT+AGG 0.521125 4_1:+25246015 None:intergenic
AGGAAGGATCCATATTCTAT+GGG 0.522964 4_1:-25246497 Msa0541380:five_prime_UTR
ATGCTTAATCCGGACCTGGT+TGG 0.524884 4_1:-25243416 Msa0541380:three_prime_UTR
ATAATCTACTCAAACAGTTC+AGG 0.531722 4_1:+25243393 None:intergenic
GTCATGCTATCAAGACCAAA+AGG 0.532159 4_1:+25246552 None:intergenic
TAGGCCATTATGAACTTGAT+GGG 0.538992 4_1:+25245402 None:intergenic
TCATCAAACAACCGAGGCGC+CGG 0.542127 4_1:+25246042 None:intergenic
ATGCTGTAGCAGCATGGATG+GGG 0.545181 4_1:-25246259 Msa0541380:exon
TTACATCTCAACCGGCGCCT+CGG 0.547408 4_1:-25246053 Msa0541380:CDS
TGTTTGAGTAGATTATGTAC+AGG 0.548216 4_1:-25243386 Msa0541380:three_prime_UTR
AGGCGCCGGTTGAGATGTAA+AGG 0.557821 4_1:+25246056 None:intergenic
CAGCTATGCGTTGTAACTTG+TGG 0.559744 4_1:-25245221 Msa0541380:exon
TGTTTAATTCTTAGTGACAC+GGG 0.562137 4_1:-25246793 Msa0541380:five_prime_UTR
GCAGGAAGCGCAGCTAAGGT+AGG 0.566794 4_1:+25246441 None:intergenic
TCCTTCACAATCTGTTGAAG+TGG 0.572914 4_1:-25246174 Msa0541380:intron
TCAAACAGTTCAGGCCAACC+AGG 0.576128 4_1:+25243402 None:intergenic
CTAGGAGCAGCAGGGACTGG+TGG 0.576994 4_1:+25246287 None:intergenic
TAATGCTGTAGCAGCATGGA+TGG 0.577022 4_1:-25246261 Msa0541380:exon
ATAGTTGTTCATATTTGGTG+AGG 0.581137 4_1:-25243575 Msa0541380:three_prime_UTR
TTGAAACTTCTGTAGTTTAG+GGG 0.592256 4_1:-25243352 Msa0541380:three_prime_UTR
AATGCTGTAGCAGCATGGAT+GGG 0.595759 4_1:-25246260 Msa0541380:exon
GTAGCAGCATGGATGGGGAA+TGG 0.596273 4_1:-25246254 Msa0541380:exon
ATACTAGAATTAATAATCAG+AGG 0.599553 4_1:+25243303 None:intergenic
GTGTTTAATTCTTAGTGACA+CGG 0.614751 4_1:-25246794 Msa0541380:five_prime_UTR
AACTTAAGAAACCAGTCCCA+TGG 0.616966 4_1:+25246576 None:intergenic
TCTGCATTAATATTAAAGGA+AGG 0.619229 4_1:-25246513 Msa0541380:five_prime_UTR
TTACAGATTGATGCTCATGA+TGG 0.621330 4_1:-25245280 Msa0541380:intron
TGGCCTACAAGAGCATTTGG+AGG 0.623760 4_1:-25245386 Msa0541380:intron
GCTGCTACAGCATTAGCACT+AGG 0.626483 4_1:+25246269 None:intergenic
AATTATCTATCTCGATAGCA+AGG 0.627140 4_1:+25246703 None:intergenic
GTGCTAATGCTGTAGCAGCA+TGG 0.633511 4_1:-25246265 Msa0541380:five_prime_UTR
TTGTGAAGGACCGGGCAATG+AGG 0.639575 4_1:+25246187 None:intergenic
TCAACAGATTGTGAAGGACC+GGG 0.651999 4_1:+25246179 None:intergenic
TATGCTCTAGTAGATGTGCT+CGG 0.653014 4_1:+25243595 None:intergenic
CAGATTGATGCTCATGATGG+TGG 0.661463 4_1:-25245277 Msa0541380:CDS
ACCACTTCAACAGATTGTGA+AGG 0.665246 4_1:+25246173 None:intergenic
CATTAGCACTAGGAGCAGCA+GGG 0.675115 4_1:+25246279 None:intergenic
AGTGATTGAGAAGACGACGA+AGG 0.704630 4_1:+25246227 None:intergenic
GCATGCTTTCCAAAACACAC+CGG 0.710220 4_1:+25243550 None:intergenic
GGAAGTTCATCAAACAACCG+AGG 0.724533 4_1:+25246036 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATAAAATTGTTATTTT+CGG - chr4_1:25243986-25244005 Msa0541380:intron 10.0%
!! TTATTATAGTACTAAATTAA+AGG + chr4_1:25244662-25244681 None:intergenic 10.0%
!! TATAATATAAGAGTTTATAT+AGG - chr4_1:25245593-25245612 Msa0541380:intron 10.0%
!!! ATTATTAATAGTAATTTTAC+CGG - chr4_1:25246356-25246375 Msa0541380:five_prime_UTR 10.0%
!! TAATATATGCAAATATTTAA+TGG - chr4_1:25246873-25246892 Msa0541380:five_prime_UTR 10.0%
!!! TAAGGTTTTTTAATTTTGTT+TGG - chr4_1:25243526-25243545 Msa0541380:three_prime_UTR 15.0%
!!! AAGGTTTTTTAATTTTGTTT+GGG - chr4_1:25243527-25243546 Msa0541380:three_prime_UTR 15.0%
!! CAAAATTAAGTTATAATAAG+TGG + chr4_1:25244041-25244060 None:intergenic 15.0%
!! TTGTTTCAATTAAACTTAAT+AGG + chr4_1:25244498-25244517 None:intergenic 15.0%
!! TTAAGTTTAATTGAAACAAT+TGG - chr4_1:25244499-25244518 Msa0541380:intron 15.0%
!!! TGATATTTTATTTTCTTTTC+AGG - chr4_1:25244899-25244918 Msa0541380:intron 15.0%
!!! AAATTTAGTAAAACTTTTGA+GGG + chr4_1:25245405-25245424 None:intergenic 15.0%
!!! TAAATTTAGTAAAACTTTTG+AGG + chr4_1:25245406-25245425 None:intergenic 15.0%
!! ATAATAGTAACTAATATTAG+AGG - chr4_1:25245523-25245542 Msa0541380:intron 15.0%
!! AAATAAAATAAGAGAATAGA+AGG - chr4_1:25245725-25245744 Msa0541380:intron 15.0%
!! AATAAAATAAGAGAATAGAA+GGG - chr4_1:25245726-25245745 Msa0541380:intron 15.0%
!! CTTTAATTAATAATAACAGT+AGG + chr4_1:25246484-25246503 None:intergenic 15.0%
!! CTACTGTTATTATTAATTAA+AGG - chr4_1:25246482-25246501 Msa0541380:five_prime_UTR 15.0%
!! TTATTATTATTAATTACCGA+CGG - chr4_1:25246566-25246585 Msa0541380:five_prime_UTR 15.0%
!! CAACAGAATTTAACAATAAA+TGG + chr4_1:25243484-25243503 None:intergenic 20.0%
!!! AGGTTTTTTAATTTTGTTTG+GGG - chr4_1:25243528-25243547 Msa0541380:three_prime_UTR 20.0%
!! AACCAATTTGAATAAGTAAA+AGG + chr4_1:25243697-25243716 None:intergenic 20.0%
!! AAAAAAAACATTAGTTACCA+TGG - chr4_1:25243766-25243785 Msa0541380:intron 20.0%
!! AAAAAAACATTAGTTACCAT+GGG - chr4_1:25243767-25243786 Msa0541380:intron 20.0%
!!! TTTATTTTAATTTACCAGCA+AGG + chr4_1:25244424-25244443 None:intergenic 20.0%
!! AAAATAACTAATCAAGTTTC+TGG + chr4_1:25244470-25244489 None:intergenic 20.0%
!!! GAAATATTCCATATTTTGAA+AGG + chr4_1:25244586-25244605 None:intergenic 20.0%
!! TAAAATGATCCTTTATGTTA+TGG - chr4_1:25244731-25244750 Msa0541380:intron 20.0%
!!! TTTTATATAGAGGAAGAAAA+TGG - chr4_1:25245625-25245644 Msa0541380:intron 20.0%
!! ATAAAATAAGAGAATAGAAG+GGG - chr4_1:25245727-25245746 Msa0541380:intron 20.0%
!! AAAACTTGTGAGTTAATTAT+GGG - chr4_1:25246038-25246057 Msa0541380:CDS 20.0%
!! TATGGTAATTACTTTGAATT+TGG - chr4_1:25246146-25246165 Msa0541380:intron 20.0%
!! ATGGTAATTACTTTGAATTT+GGG - chr4_1:25246147-25246166 Msa0541380:intron 20.0%
!! TGTTATTATTAATTAAAGGC+AGG - chr4_1:25246486-25246505 Msa0541380:five_prime_UTR 20.0%
!! GTTATTATTAATTAAAGGCA+GGG - chr4_1:25246487-25246506 Msa0541380:five_prime_UTR 20.0%
!! ATACTAGAATTAATAATCAG+AGG + chr4_1:25247059-25247078 None:intergenic 20.0%
! TGATTAATAATGCTCTAGTT+TGG - chr4_1:25243320-25243339 Msa0541380:three_prime_UTR 25.0%
!! GATTAATAATGCTCTAGTTT+GGG - chr4_1:25243321-25243340 Msa0541380:three_prime_UTR 25.0%
! TTAAGCACTTAAAAACCAAA+GGG + chr4_1:25243631-25243650 None:intergenic 25.0%
! ATTAAGCACTTAAAAACCAA+AGG + chr4_1:25243632-25243651 None:intergenic 25.0%
!! GACCTTTTACTTATTCAAAT+TGG - chr4_1:25243692-25243711 Msa0541380:intron 25.0%
!! TTTTATGTTTACAGTGTGTT+TGG - chr4_1:25243715-25243734 Msa0541380:intron 25.0%
! GACATCTGCATTAATATTAA+AGG - chr4_1:25243842-25243861 Msa0541380:intron 25.0%
! TCTGCATTAATATTAAAGGA+AGG - chr4_1:25243846-25243865 Msa0541380:intron 25.0%
! AATTGAAATCCCATAGAATA+TGG + chr4_1:25243874-25243893 None:intergenic 25.0%
! ATTTCAATTTCACCTAGAAA+TGG - chr4_1:25243885-25243904 Msa0541380:intron 25.0%
! TTTCGGTTGTTTATATACAT+TGG - chr4_1:25244003-25244022 Msa0541380:intron 25.0%
! AAAATGGAGAAATTTCACTT+TGG - chr4_1:25244527-25244546 Msa0541380:intron 25.0%
! AAATGGAGAAATTTCACTTT+GGG - chr4_1:25244528-25244547 Msa0541380:intron 25.0%
! TATCAAAGCCTTTCAAAATA+TGG - chr4_1:25244575-25244594 Msa0541380:intron 25.0%
! ATGGAATATTTCCAATTGTT+CGG - chr4_1:25244594-25244613 Msa0541380:intron 25.0%
!! AGTAAAATGATCTTTTGAAC+AGG - chr4_1:25244762-25244781 Msa0541380:intron 25.0%
!!! GCTGGTTTTTTTAAATGAAA+AGG + chr4_1:25245122-25245141 None:intergenic 25.0%
! TGGAGATGATAAGTTAATTA+AGG - chr4_1:25245158-25245177 Msa0541380:intron 25.0%
!! GGTATTACATAGTTATTTCA+TGG + chr4_1:25245384-25245403 None:intergenic 25.0%
!!! GCATTATCACTTTTATATAG+AGG - chr4_1:25245615-25245634 Msa0541380:intron 25.0%
! TAAAATAAGAGAATAGAAGG+GGG - chr4_1:25245728-25245747 Msa0541380:intron 25.0%
! TAGAGATTTCATGAAATTAG+AGG - chr4_1:25245848-25245867 Msa0541380:intron 25.0%
! AACATGCAATAAGGAATATA+TGG - chr4_1:25245900-25245919 Msa0541380:intron 25.0%
! GAAAACTTGTGAGTTAATTA+TGG - chr4_1:25246037-25246056 Msa0541380:CDS 25.0%
! AAACTTGTGAGTTAATTATG+GGG - chr4_1:25246039-25246058 Msa0541380:CDS 25.0%
!!! TCATTCATTGATTCTTTAGA+AGG - chr4_1:25246122-25246141 Msa0541380:intron 25.0%
!!! CATTCATTGATTCTTTAGAA+GGG - chr4_1:25246123-25246142 Msa0541380:intron 25.0%
! TGGTAATTACTTTGAATTTG+GGG - chr4_1:25246148-25246167 Msa0541380:intron 25.0%
! TTAAATTGTTGGAACAATGT+TGG - chr4_1:25246217-25246236 Msa0541380:CDS 25.0%
! TAAATTGTTGGAACAATGTT+GGG - chr4_1:25246218-25246237 Msa0541380:CDS 25.0%
! GTATCTGTAGATAATAACAT+TGG - chr4_1:25246285-25246304 Msa0541380:five_prime_UTR 25.0%
!! ATTAATAGTAATTTTACCGG+CGG - chr4_1:25246359-25246378 Msa0541380:five_prime_UTR 25.0%
! CCTCTATATGTGTTAAATAT+AGG + chr4_1:25246451-25246470 None:intergenic 25.0%
! CCTATATTTAACACATATAG+AGG - chr4_1:25246448-25246467 Msa0541380:five_prime_UTR 25.0%
! ATATTTAACACATATAGAGG+CGG - chr4_1:25246451-25246470 Msa0541380:five_prime_UTR 25.0%
!! TTACATGTGATTATGTCTTT+TGG - chr4_1:25246845-25246864 Msa0541380:five_prime_UTR 25.0%
!! TACATGTGATTATGTCTTTT+GGG - chr4_1:25246846-25246865 Msa0541380:five_prime_UTR 25.0%
!!! TTTTGAAACTTCTGTAGTTT+AGG - chr4_1:25247005-25247024 Msa0541380:five_prime_UTR 25.0%
!! TTTGAAACTTCTGTAGTTTA+GGG - chr4_1:25247006-25247025 Msa0541380:five_prime_UTR 25.0%
CCAACAATGTCTTAGAATAA+TGG + chr4_1:25243382-25243401 None:intergenic 30.0%
!! CCATTATTCTAAGACATTGT+TGG - chr4_1:25243379-25243398 Msa0541380:three_prime_UTR 30.0%
TAATGTACGTATGAACTAGA+TGG + chr4_1:25243436-25243455 None:intergenic 30.0%
AAAAACCTTAATCCTTCATG+AGG + chr4_1:25243516-25243535 None:intergenic 30.0%
GTGTTTAATTCTTAGTGACA+CGG - chr4_1:25243565-25243584 Msa0541380:three_prime_UTR 30.0%
TGTTTAATTCTTAGTGACAC+GGG - chr4_1:25243566-25243585 Msa0541380:three_prime_UTR 30.0%
AATTATCTATCTCGATAGCA+AGG + chr4_1:25243659-25243678 None:intergenic 30.0%
! TGGTTTCTTAAGTTTCCTTT+TGG - chr4_1:25243792-25243811 Msa0541380:intron 30.0%
CTGAAATTGCATTATGAACT+TGG - chr4_1:25243940-25243959 Msa0541380:intron 30.0%
CTATATTGACAACTCTTTAC+AGG - chr4_1:25244262-25244281 Msa0541380:intron 30.0%
ATATACCTTTACATCTCAAC+CGG - chr4_1:25244298-25244317 Msa0541380:intron 30.0%
AACTTGCAAACAATAGTTGT+AGG + chr4_1:25244347-25244366 None:intergenic 30.0%
ATTGTTTGCAAGTTACATCA+AGG - chr4_1:25244353-25244372 Msa0541380:intron 30.0%
AGGATCAACTGTAACAAATA+TGG - chr4_1:25244373-25244392 Msa0541380:intron 30.0%
!! GAAACAATTGGTAGTGAAAA+TGG - chr4_1:25244511-25244530 Msa0541380:intron 30.0%
AATGATCCTTTATGTTATGG+TGG - chr4_1:25244734-25244753 Msa0541380:intron 30.0%
TAAACTCCACCATAACATAA+AGG + chr4_1:25244743-25244762 None:intergenic 30.0%
CTTTGAATTTGAAAGAATCG+TGG - chr4_1:25244785-25244804 Msa0541380:intron 30.0%
AAGCATTAATCAAGCAACAT+CGG + chr4_1:25244877-25244896 None:intergenic 30.0%
TATACCCATCAAGTTCATAA+TGG - chr4_1:25244953-25244972 Msa0541380:intron 30.0%
! TCCTAGTGTTTTATCTGTTT+TGG - chr4_1:25245291-25245310 Msa0541380:intron 30.0%
ACCAAAACAGATAAAACACT+AGG + chr4_1:25245295-25245314 None:intergenic 30.0%
AGACTGAAGTTTCAAAATAG+TGG + chr4_1:25245318-25245337 None:intergenic 30.0%
TGTAGATCAAGTTTATTGCT+GGG - chr4_1:25245871-25245890 Msa0541380:intron 30.0%
TAAGGAATATATGGCTGAAT+TGG - chr4_1:25245909-25245928 Msa0541380:intron 30.0%
AAGGAATATATGGCTGAATT+GGG - chr4_1:25245910-25245929 Msa0541380:intron 30.0%
! AACAAGGATTTGTGTTGATA+CGG - chr4_1:25245965-25245984 Msa0541380:CDS 30.0%
GAAGCTTAAAGTGATAACAT+AGG + chr4_1:25246077-25246096 None:intergenic 30.0%
! ATTGATTCTTTAGAAGGGTA+TGG - chr4_1:25246128-25246147 Msa0541380:intron 30.0%
! TGAATTTCGTTGGATACTTT+TGG - chr4_1:25246190-25246209 Msa0541380:CDS 30.0%
! GAATTTCGTTGGATACTTTT+GGG - chr4_1:25246191-25246210 Msa0541380:CDS 30.0%
!! CTTTTGGGAGTTTAAATTGT+TGG - chr4_1:25246206-25246225 Msa0541380:CDS 30.0%
! TTAATAGTAATTTTACCGGC+GGG - chr4_1:25246360-25246379 Msa0541380:five_prime_UTR 30.0%
TATTTAACACATATAGAGGC+GGG - chr4_1:25246452-25246471 Msa0541380:five_prime_UTR 30.0%
TGTCTCTAAGTTTCAATAGT+CGG + chr4_1:25246529-25246548 None:intergenic 30.0%
TAACGAGTAATATAACAAGC+AGG + chr4_1:25246679-25246698 None:intergenic 30.0%
AGAGCATAGTTGTTCATATT+TGG - chr4_1:25246779-25246798 Msa0541380:five_prime_UTR 30.0%
! ATAGTTGTTCATATTTGGTG+AGG - chr4_1:25246784-25246803 Msa0541380:five_prime_UTR 30.0%
! ACATGTGATTATGTCTTTTG+GGG - chr4_1:25246847-25246866 Msa0541380:five_prime_UTR 30.0%
ATAATCTACTCAAACAGTTC+AGG + chr4_1:25246969-25246988 None:intergenic 30.0%
TGTTTGAGTAGATTATGTAC+AGG - chr4_1:25246973-25246992 Msa0541380:five_prime_UTR 30.0%
TTGAAACTTCTGTAGTTTAG+GGG - chr4_1:25247007-25247026 Msa0541380:five_prime_UTR 30.0%
TAATATCTTCAAACCTTGCC+AGG + chr4_1:25243409-25243428 None:intergenic 35.0%
! GCTGGTACATGATTGTATTT+TGG - chr4_1:25243594-25243613 Msa0541380:three_prime_UTR 35.0%
TTGGACAAATTACTACCCTT+TGG - chr4_1:25243613-25243632 Msa0541380:three_prime_UTR 35.0%
TGTTTACAGTGTGTTTGGTT+AGG - chr4_1:25243720-25243739 Msa0541380:intron 35.0%
ATAGCATGACTGCAGTTATA+AGG - chr4_1:25243820-25243839 Msa0541380:intron 35.0%
AAGGAAGGATCCATATTCTA+TGG - chr4_1:25243861-25243880 Msa0541380:intron 35.0%
AGGAAGGATCCATATTCTAT+GGG - chr4_1:25243862-25243881 Msa0541380:intron 35.0%
GTTCATAATGCAATTTCAGC+AGG + chr4_1:25243939-25243958 None:intergenic 35.0%
GCAAGGATAGAATGAATTGA+TGG + chr4_1:25244407-25244426 None:intergenic 35.0%
TCAATTCATTCTATCCTTGC+TGG - chr4_1:25244407-25244426 Msa0541380:intron 35.0%
! AAATTTCACTTTGGGAAGCA+AGG - chr4_1:25244536-25244555 Msa0541380:intron 35.0%
CTGAAATTTGACCGAACAAT+TGG + chr4_1:25244608-25244627 None:intergenic 35.0%
CAATTGTTCGGTCAAATTTC+AGG - chr4_1:25244606-25244625 Msa0541380:intron 35.0%
TATCCATCAATCGTGTATCA+TGG - chr4_1:25244809-25244828 Msa0541380:intron 35.0%
TTACCAATAAAACACGCATC+CGG + chr4_1:25244838-25244857 None:intergenic 35.0%
TAGGCCATTATGAACTTGAT+GGG + chr4_1:25244960-25244979 None:intergenic 35.0%
TTACAGATTGATGCTCATGA+TGG - chr4_1:25245079-25245098 Msa0541380:intron 35.0%
TGTCTCATTCTGTGTTTGAA+TGG - chr4_1:25245215-25245234 Msa0541380:exon 35.0%
ATAGAGGAAGAAAATGGTCT+TGG - chr4_1:25245631-25245650 Msa0541380:intron 35.0%
! TGGCTTTTCAGCAACTAATT+GGG + chr4_1:25245688-25245707 None:intergenic 35.0%
! ATGGCTTTTCAGCAACTAAT+TGG + chr4_1:25245689-25245708 None:intergenic 35.0%
GTGTAGATCAAGTTTATTGC+TGG - chr4_1:25245870-25245889 Msa0541380:intron 35.0%
! TTTTCTAAGTTGCTGAGAAG+TGG + chr4_1:25246022-25246041 None:intergenic 35.0%
GTCAAATGCATGAATTTCGT+TGG - chr4_1:25246180-25246199 Msa0541380:CDS 35.0%
TGAAGAATGTCATCAGAACA+TGG - chr4_1:25246254-25246273 Msa0541380:exon 35.0%
! TAATAGTAATTTTACCGGCG+GGG - chr4_1:25246361-25246380 Msa0541380:five_prime_UTR 35.0%
ATTTAACACATATAGAGGCG+GGG - chr4_1:25246453-25246472 Msa0541380:five_prime_UTR 35.0%
CATACATATAAACTCTCCGT+CGG + chr4_1:25246585-25246604 None:intergenic 35.0%
ATTACTCGTTATCTACTCAC+TGG - chr4_1:25246688-25246707 Msa0541380:five_prime_UTR 35.0%
! CATCAAGAGCAAAGTTGTTT+TGG + chr4_1:25246926-25246945 None:intergenic 35.0%
TTTGCTCTTGATGCTTAATC+CGG - chr4_1:25246933-25246952 Msa0541380:five_prime_UTR 35.0%
! TAAGACATTGTTGGTTGTCC+TGG - chr4_1:25243388-25243407 Msa0541380:three_prime_UTR 40.0%
TTGTGTTCTTTGCCTCATGA+AGG - chr4_1:25243501-25243520 Msa0541380:three_prime_UTR 40.0%
CTTTGCCTCATGAAGGATTA+AGG - chr4_1:25243508-25243527 Msa0541380:three_prime_UTR 40.0%
AACATTAGTTACCATGGGAC+TGG - chr4_1:25243772-25243791 Msa0541380:intron 40.0%
AACTTAAGAAACCAGTCCCA+TGG + chr4_1:25243786-25243805 None:intergenic 40.0%
GTCATGCTATCAAGACCAAA+AGG + chr4_1:25243810-25243829 None:intergenic 40.0%
TTCAACAGATTGTGAAGGAC+CGG + chr4_1:25244184-25244203 None:intergenic 40.0%
TCCTTCACAATCTGTTGAAG+TGG - chr4_1:25244185-25244204 Msa0541380:intron 40.0%
ACCACTTCAACAGATTGTGA+AGG + chr4_1:25244189-25244208 None:intergenic 40.0%
TACCAATAAAACACGCATCC+GGG + chr4_1:25244837-25244856 None:intergenic 40.0%
GTAGGCCATTATGAACTTGA+TGG + chr4_1:25244961-25244980 None:intergenic 40.0%
!! TAATGGCCTACAAGAGCATT+TGG - chr4_1:25244970-25244989 Msa0541380:intron 40.0%
ATACCTCCAAATGCTCTTGT+AGG + chr4_1:25244979-25244998 None:intergenic 40.0%
!! TGATGGTGGTGTTAATGACT+TGG - chr4_1:25245096-25245115 Msa0541380:intron 40.0%
! AAGAAAATGGTCTTGGTGAC+AGG - chr4_1:25245638-25245657 Msa0541380:intron 40.0%
! AGAAAATGGTCTTGGTGACA+GGG - chr4_1:25245639-25245658 Msa0541380:intron 40.0%
ATAGAAGGGGGAAAAAGAGA+AGG - chr4_1:25245740-25245759 Msa0541380:intron 40.0%
TAGAAGGGGGAAAAAGAGAA+GGG - chr4_1:25245741-25245760 Msa0541380:intron 40.0%
GAGTTAGAAAGGGAAACGAA+GGG - chr4_1:25245771-25245790 Msa0541380:intron 40.0%
GGGTAGTGTAACATGCAATA+AGG - chr4_1:25245891-25245910 Msa0541380:intron 40.0%
ATGGCTGAATTGGGTTACTT+TGG - chr4_1:25245919-25245938 Msa0541380:intron 40.0%
! TGGCTGAATTGGGTTACTTT+GGG - chr4_1:25245920-25245939 Msa0541380:intron 40.0%
!! TGGGTTACTTTGGGATACTA+AGG - chr4_1:25245929-25245948 Msa0541380:intron 40.0%
TGGGATACTAAGGATAGAAG+AGG - chr4_1:25245939-25245958 Msa0541380:intron 40.0%
GGGATACTAAGGATAGAAGA+GGG - chr4_1:25245940-25245959 Msa0541380:intron 40.0%
TGTTGGAACAATGTTGGGTT+TGG - chr4_1:25246223-25246242 Msa0541380:CDS 40.0%
AGCCTATCGTCACTATTTAC+AGG - chr4_1:25246400-25246419 Msa0541380:five_prime_UTR 40.0%
CTATCGTCACTATTTACAGG+CGG - chr4_1:25246403-25246422 Msa0541380:five_prime_UTR 40.0%
TATCGTCACTATTTACAGGC+GGG - chr4_1:25246404-25246423 Msa0541380:five_prime_UTR 40.0%
TTTAACACATATAGAGGCGG+GGG - chr4_1:25246454-25246473 Msa0541380:five_prime_UTR 40.0%
TATGCTCTAGTAGATGTGCT+CGG + chr4_1:25246767-25246786 None:intergenic 40.0%
! GTTCATATTTGGTGAGGTTC+CGG - chr4_1:25246790-25246809 Msa0541380:five_prime_UTR 40.0%
TTAGTGACACGGGAAATTGC+TGG - chr4_1:25243576-25243595 Msa0541380:three_prime_UTR 45.0%
TAATGCTGTAGCAGCATGGA+TGG - chr4_1:25244098-25244117 Msa0541380:intron 45.0%
AATGCTGTAGCAGCATGGAT+GGG - chr4_1:25244099-25244118 Msa0541380:intron 45.0%
AGTGATTGAGAAGACGACGA+AGG + chr4_1:25244135-25244154 None:intergenic 45.0%
TCAACAGATTGTGAAGGACC+GGG + chr4_1:25244183-25244202 None:intergenic 45.0%
GGAAGTTCATCAAACAACCG+AGG + chr4_1:25244326-25244345 None:intergenic 45.0%
GAGTGATGAATGCACTGCTA+TGG - chr4_1:25244705-25244724 Msa0541380:intron 45.0%
GCTCCATGATACACGATTGA+TGG + chr4_1:25244815-25244834 None:intergenic 45.0%
CAGATTGATGCTCATGATGG+TGG - chr4_1:25245082-25245101 Msa0541380:intron 45.0%
CACAAGTTACAACGCATAGC+TGG + chr4_1:25245140-25245159 None:intergenic 45.0%
CAGCTATGCGTTGTAACTTG+TGG - chr4_1:25245138-25245157 Msa0541380:intron 45.0%
! GGAAAAAGAGAAGGGTACGA+GGG - chr4_1:25245749-25245768 Msa0541380:intron 45.0%
GGAGTTAGAAAGGGAAACGA+AGG - chr4_1:25245770-25245789 Msa0541380:intron 45.0%
AGGATAGAAGAGGGAGAACA+AGG - chr4_1:25245949-25245968 Msa0541380:intron 45.0%
!! AGTGGATTGAGAACTGATGC+TGG + chr4_1:25246004-25246023 None:intergenic 45.0%
CGCCTGTAAATAGTGACGAT+AGG + chr4_1:25246405-25246424 None:intergenic 45.0%
ATCGTCACTATTTACAGGCG+GGG - chr4_1:25246405-25246424 Msa0541380:five_prime_UTR 45.0%
TTAACACATATAGAGGCGGG+GGG - chr4_1:25246455-25246474 Msa0541380:five_prime_UTR 45.0%
AATTAAAGGCAGGGAGCCTA+AGG - chr4_1:25246496-25246515 Msa0541380:five_prime_UTR 45.0%
GCATGCTTTCCAAAACACAC+CGG + chr4_1:25246812-25246831 None:intergenic 45.0%
!!! ATTACTTTTATTTATATTTT+TGG - chr4_1:25243452-25243471 Msa0541380:three_prime_UTR 5.0%
!!! TTACTTTTATTTATATTTTT+GGG - chr4_1:25243453-25243472 Msa0541380:three_prime_UTR 5.0%
!!! TTTTTTTATTTTTTTCTTAA+TGG + chr4_1:25245708-25245727 None:intergenic 5.0%
! CATTGTTGGTTGTCCTGGCA+AGG - chr4_1:25243393-25243412 Msa0541380:three_prime_UTR 50.0%
GGCGTGCAATGTCCATTTCT+AGG + chr4_1:25243900-25243919 None:intergenic 50.0%
CATTAGCACTAGGAGCAGCA+GGG + chr4_1:25244083-25244102 None:intergenic 50.0%
GCTGCTACAGCATTAGCACT+AGG + chr4_1:25244093-25244112 None:intergenic 50.0%
GTGCTAATGCTGTAGCAGCA+TGG - chr4_1:25244094-25244113 Msa0541380:intron 50.0%
ATGCTGTAGCAGCATGGATG+GGG - chr4_1:25244100-25244119 Msa0541380:intron 50.0%
CAATCGTGTATCATGGAGCC+CGG - chr4_1:25244816-25244835 Msa0541380:intron 50.0%
! AGCCCGGATGCGTGTTTTAT+TGG - chr4_1:25244832-25244851 Msa0541380:intron 50.0%
!! TGGCCTACAAGAGCATTTGG+AGG - chr4_1:25244973-25244992 Msa0541380:intron 50.0%
! GGGAAAAAGAGAAGGGTACG+AGG - chr4_1:25245748-25245767 Msa0541380:intron 50.0%
AGGGTACGAGGGAGTTAGAA+AGG - chr4_1:25245760-25245779 Msa0541380:intron 50.0%
GGGTACGAGGGAGTTAGAAA+GGG - chr4_1:25245761-25245780 Msa0541380:intron 50.0%
TAACACATATAGAGGCGGGG+GGG - chr4_1:25246456-25246475 Msa0541380:five_prime_UTR 50.0%
CTTGATGCTTAATCCGGACC+TGG - chr4_1:25246939-25246958 Msa0541380:five_prime_UTR 50.0%
ATGCTTAATCCGGACCTGGT+TGG - chr4_1:25246943-25246962 Msa0541380:five_prime_UTR 50.0%
TCAAACAGTTCAGGCCAACC+AGG + chr4_1:25246960-25246979 None:intergenic 50.0%
! TTCAGCAGGAAGCGCAGCTA+AGG + chr4_1:25243925-25243944 None:intergenic 55.0%
GCATTAGCACTAGGAGCAGC+AGG + chr4_1:25244084-25244103 None:intergenic 55.0%
GTAGCAGCATGGATGGGGAA+TGG - chr4_1:25244105-25244124 Msa0541380:intron 55.0%
TTGTGAAGGACCGGGCAATG+AGG + chr4_1:25244175-25244194 None:intergenic 55.0%
!! AGGCGCCGGTTGAGATGTAA+AGG + chr4_1:25244306-25244325 None:intergenic 55.0%
TTACATCTCAACCGGCGCCT+CGG - chr4_1:25244306-25244325 Msa0541380:intron 55.0%
TCATCAAACAACCGAGGCGC+CGG + chr4_1:25244320-25244339 None:intergenic 55.0%
GGGAAGCAAGGTTACGAGAG+AGG - chr4_1:25244548-25244567 Msa0541380:intron 55.0%
GAAAGGGAAACGAAGGGAGC+TGG - chr4_1:25245777-25245796 Msa0541380:intron 55.0%
ATAGGCTCCCCGCCTTAGTT+AGG + chr4_1:25246433-25246452 None:intergenic 55.0%
TAAAGGCAGGGAGCCTAAGG+CGG - chr4_1:25246499-25246518 Msa0541380:five_prime_UTR 55.0%
! GGTGAGGTTCCGGTGTGTTT+TGG - chr4_1:25246800-25246819 Msa0541380:five_prime_UTR 55.0%
! GCAGGAAGCGCAGCTAAGGT+AGG + chr4_1:25243921-25243940 None:intergenic 60.0%
! GCAGCAGGGACTGGTGGAAA+TGG + chr4_1:25244069-25244088 None:intergenic 60.0%
CTCGCAGCTTCCTCATTGCC+CGG - chr4_1:25244162-25244181 Msa0541380:intron 60.0%
CGATAGGCTCCCCGTCAAAG+TGG + chr4_1:25246389-25246408 None:intergenic 60.0%
ACAGGCGGGGAGCCTAACTA+AGG - chr4_1:25246418-25246437 Msa0541380:five_prime_UTR 60.0%
AAAGGCAGGGAGCCTAAGGC+GGG - chr4_1:25246500-25246519 Msa0541380:five_prime_UTR 60.0%
! AATAGTCGGCTCCCCGCCTT+AGG + chr4_1:25246515-25246534 None:intergenic 60.0%
CTCGGTTCTGCACGTGCTAG+TGG + chr4_1:25246749-25246768 None:intergenic 60.0%
CAGTTCAGGCCAACCAGGTC+CGG + chr4_1:25246955-25246974 None:intergenic 60.0%
CTAGGAGCAGCAGGGACTGG+TGG + chr4_1:25244075-25244094 None:intergenic 65.0%
GCACTAGGAGCAGCAGGGAC+TGG + chr4_1:25244078-25244097 None:intergenic 65.0%
GGCGGGGAGCCTAACTAAGG+CGG - chr4_1:25246421-25246440 Msa0541380:five_prime_UTR 65.0%
GCGGGGAGCCTAACTAAGGC+GGG - chr4_1:25246422-25246441 Msa0541380:five_prime_UTR 65.0%
CGGGGAGCCTAACTAAGGCG+GGG - chr4_1:25246423-25246442 Msa0541380:five_prime_UTR 65.0%
AAGGCAGGGAGCCTAAGGCG+GGG - chr4_1:25246501-25246520 Msa0541380:five_prime_UTR 65.0%
CCGTCAAAGTGGCTCCCCGC+CGG + chr4_1:25246378-25246397 None:intergenic 70.0%
! CCGGCGGGGAGCCACTTTGA+CGG - chr4_1:25246375-25246394 Msa0541380:five_prime_UTR 70.0%
! CGGCGGGGAGCCACTTTGAC+GGG - chr4_1:25246376-25246395 Msa0541380:five_prime_UTR 70.0%
! GGCGGGGAGCCACTTTGACG+GGG - chr4_1:25246377-25246396 Msa0541380:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 25243271 25247110 25243271 ID=Msa0541380;Name=Msa0541380
chr4_1 mRNA 25243271 25247110 25243271 ID=Msa0541380-mRNA-1;Parent=Msa0541380;Name=Msa0541380-mRNA-1;_AED=0.15;_eAED=0.15;_QI=846|0.75|1|1|0.75|0.6|5|393|125
chr4_1 exon 25243271 25243637 25243271 ID=Msa0541380-mRNA-1:exon:6794;Parent=Msa0541380-mRNA-1
chr4_1 exon 25245202 25245296 25245202 ID=Msa0541380-mRNA-1:exon:6793;Parent=Msa0541380-mRNA-1
chr4_1 exon 25245387 25245460 25245387 ID=Msa0541380-mRNA-1:exon:6792;Parent=Msa0541380-mRNA-1
chr4_1 exon 25245953 25246097 25245953 ID=Msa0541380-mRNA-1:exon:6791;Parent=Msa0541380-mRNA-1
chr4_1 exon 25246175 25247110 25246175 ID=Msa0541380-mRNA-1:exon:6790;Parent=Msa0541380-mRNA-1
chr4_1 five_prime_UTR 25246265 25247110 25246265 ID=Msa0541380-mRNA-1:five_prime_utr;Parent=Msa0541380-mRNA-1
chr4_1 CDS 25246175 25246264 25246175 ID=Msa0541380-mRNA-1:cds;Parent=Msa0541380-mRNA-1
chr4_1 CDS 25245953 25246097 25245953 ID=Msa0541380-mRNA-1:cds;Parent=Msa0541380-mRNA-1
chr4_1 CDS 25245387 25245460 25245387 ID=Msa0541380-mRNA-1:cds;Parent=Msa0541380-mRNA-1
chr4_1 CDS 25245228 25245296 25245228 ID=Msa0541380-mRNA-1:cds;Parent=Msa0541380-mRNA-1
chr4_1 three_prime_UTR 25245202 25245227 25245202 ID=Msa0541380-mRNA-1:three_prime_utr;Parent=Msa0541380-mRNA-1
chr4_1 three_prime_UTR 25243271 25243637 25243271 ID=Msa0541380-mRNA-1:three_prime_utr;Parent=Msa0541380-mRNA-1
Gene Sequence

>Msa0541380

ATGGGGAATGGAAATCCTTCGTCGTCTTCTCAATCACTTGCTGCGCTCGCAGCTTCCTCATTGCCCGGTCCTTCACAATCTGTTGAAGTGGCTGTGAAAGTTGCATATACCTTTACATCTCAACCGGCGCCTCGGTTGTTTGATGAACTTCCTACAACTATTGTTTGCAAGTTACATCAAGGATCAACTGTAACAAATATGGAGTTTCATCCATCAATTCATTCTATCCTTGCTGGTGTTGCTTTTGCAAAACATTTGATTCATTTATATACCCATCAAGTTCATAATGGCCTACAAGAGCATTTGGAGATTGATGCTCATGATGGTGGTGTTAATGACTTGGCCTTTTCATTTAAAAAAACCAGCTATGCGTTGTAA
Protein sequence

>Msa0541380

MGNGNPSSSSQSLAALAASSLPGPSQSVEVAVKVAYTFTSQPAPRLFDELPTTIVCKLHQGSTVTNMEFHPSIHSILAGVAFAKHLIHLYTHQVHNGLQEHLEIDAHDGGVNDLAFSFKKTSYAL*