AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0541640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541640 MtrunA17_Chr8g0385881 97.143 140 3 1 1 140 1 139 1.59e-94 269
Msa0541640 MtrunA17_Chr2g0286471 46.853 143 67 4 1 139 1 138 3.99e-37 123
Msa0541640 MtrunA17_Chr2g0286481 51.220 123 53 4 22 139 18 138 6.97e-34 115
Msa0541640 MtrunA17_Chr1g0191251 50.000 80 35 2 57 134 10 86 5.69e-18 73.6
Msa0541640 MtrunA17_Chr7g0249801 45.833 72 37 2 62 132 42 112 1.56e-11 57.8
Msa0541640 MtrunA17_Chr7g0249561 41.772 79 44 2 62 139 53 130 4.58e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541640 AT4G25050.1 55.000 140 53 4 1 139 1 131 5.73e-39 128
Msa0541640 AT4G25050.2 50.658 152 53 5 1 139 1 143 2.28e-35 120
Msa0541640 AT3G05020.1 49.306 144 60 5 1 139 1 136 4.42e-34 116
Msa0541640 AT5G27200.1 63.529 85 29 1 55 139 55 137 2.17e-31 109
Msa0541640 AT1G54630.1 41.333 150 58 5 1 139 4 134 7.41e-28 100
Msa0541640 AT1G54580.1 57.647 85 34 1 55 139 52 134 1.33e-26 97.4
Msa0541640 AT1G65290.1 41.772 79 44 2 62 139 49 126 2.06e-11 57.8
Msa0541640 AT1G54630.2 53.448 58 25 1 55 112 52 107 2.99e-11 57.0

Find 54 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACAAAATTGGCTTATTA+TGG 0.159879 4_1:+25628842 None:intergenic
AGTTCTGAAAACTTGGTTTC+TGG 0.187983 4_1:+25627625 None:intergenic
TCTGGGGTAAGCTTGGTTTC+AGG 0.252983 4_1:+25627643 None:intergenic
CATTATCATCCTCAATATTA+AGG 0.281641 4_1:+25627280 None:intergenic
TGTGCTTATGCGTATTCTTT+TGG 0.292912 4_1:-25627098 Msa0541640:three_prime_UTR
TCTTTCAGAACTTCAAGTTT+TGG 0.316075 4_1:-25627887 Msa0541640:CDS
CTCACTTTCTCCACCGTCTC+AGG 0.323599 4_1:+25627697 None:intergenic
AATACGCATAAGCACAAATA+AGG 0.337832 4_1:+25627105 None:intergenic
AAAGAAAGATTAAGCTTAAG+AGG 0.355256 4_1:-25627202 Msa0541640:exon
GAACAAAATTGGCTTATTAT+GGG 0.362412 4_1:+25628843 None:intergenic
AGATAGAATAGTAAGAGATA+TGG 0.363474 4_1:+25628729 None:intergenic
AATACATTGTGAACTGAGTT+AGG 0.370219 4_1:-25627154 Msa0541640:three_prime_UTR
AGGCCATTGTATTGAAGCTT+AGG 0.372070 4_1:+25628702 None:intergenic
GGAAATAGTGATGGGTTTAG+AGG 0.377032 4_1:-25627313 Msa0541640:CDS
AGCCATGTCAGGAACAAAAT+TGG 0.386576 4_1:+25628832 None:intergenic
GTTCTGAAAACTTGGTTTCT+GGG 0.388553 4_1:+25627626 None:intergenic
AGCACCAAGTTCTGAAAACT+TGG 0.401955 4_1:+25627618 None:intergenic
AGAATTTAACCTTAATATTG+AGG 0.419128 4_1:-25627289 Msa0541640:CDS
TTATCTAGCTGGTGCTTAGT+TGG 0.422462 4_1:+25628802 None:intergenic
CTTGGTTTCTGGGGTAAGCT+TGG 0.444178 4_1:+25627636 None:intergenic
TTTGATCAAGTATAGAATAT+GGG 0.453729 4_1:-25626891 Msa0541640:three_prime_UTR
GAATAGTAAGAGATATGGAT+TGG 0.457105 4_1:+25628734 None:intergenic
TATGGATTGGGTTGATGAAA+CGG 0.466360 4_1:+25628747 None:intergenic
GCTAGACCAGATGTCGCAAT+TGG 0.471348 4_1:+25626812 None:intergenic
AGCTTAAGAGGTGAACCTAA+TGG 0.475146 4_1:-25627190 Msa0541640:three_prime_UTR
CAAATCAGCTGCTTGTTGAA+TGG 0.477053 4_1:+25627242 None:intergenic
CCTCTTTCAATTTCTCTACA+AGG 0.482096 4_1:-25628649 Msa0541640:CDS
TTCTGAAAACTTGGTTTCTG+GGG 0.489485 4_1:+25627627 None:intergenic
AAAAGAAGTAAGAGGGAGGG+AGG 0.502298 4_1:-25626850 Msa0541640:three_prime_UTR
TTGGAAAGTAAAGCCATGTC+AGG 0.506514 4_1:+25628821 None:intergenic
GAAATTGAAAGAGGGTTGAA+AGG 0.517599 4_1:+25628658 None:intergenic
AGATAAGTGTGTTATCTAGC+TGG 0.519327 4_1:+25628791 None:intergenic
CCTTGTAGAGAAATTGAAAG+AGG 0.526756 4_1:+25628649 None:intergenic
CGATGAGAAGATCTCAAAGA+AGG 0.527124 4_1:+25627830 None:intergenic
AATAGTAAGAGATATGGATT+GGG 0.536153 4_1:+25628735 None:intergenic
TTCAAAAGAAGTAAGAGGGA+GGG 0.537058 4_1:-25626853 Msa0541640:three_prime_UTR
AGAGGGTTGAAAGGTAAGAG+AGG 0.538850 4_1:+25628667 None:intergenic
AAGAGAGGTGGCTGAAAGAG+AGG 0.547136 4_1:+25628682 None:intergenic
GCAATTTAACCAAGTTACAA+TGG 0.553844 4_1:+25627050 None:intergenic
GGGTTGAAAGGTAAGAGAGG+TGG 0.561476 4_1:+25628670 None:intergenic
TTCCATCCTTGTGCCTAATG+CGG 0.567443 4_1:-25626926 Msa0541640:three_prime_UTR
CTTCCTAAGCTTCAATACAA+TGG 0.568743 4_1:-25628705 Msa0541640:exon
TGAACCTAATGGAAGCTCTA+CGG 0.570264 4_1:-25627179 Msa0541640:three_prime_UTR
GTGGCAGGTGGAAATAGTGA+TGG 0.572304 4_1:-25627322 Msa0541640:intron
CTTGTAGAGAAATTGAAAGA+GGG 0.583035 4_1:+25628650 None:intergenic
AACCGCATTAGGCACAAGGA+TGG 0.589368 4_1:+25626924 None:intergenic
AAGTATAGAATATGGGTACA+TGG 0.592357 4_1:-25626884 Msa0541640:three_prime_UTR
TTTCTCTACAAGGACTAACC+AGG 0.601936 4_1:-25628639 Msa0541640:intron
TCAGGCACAACCTGAGACGG+TGG 0.607017 4_1:-25627707 Msa0541640:CDS
GATTCAGGCACAACCTGAGA+CGG 0.613003 4_1:-25627710 Msa0541640:CDS
TGGCAGGTGGAAATAGTGAT+GGG 0.642802 4_1:-25627321 Msa0541640:intron
TTTCAAAAGAAGTAAGAGGG+AGG 0.646068 4_1:-25626854 Msa0541640:three_prime_UTR
TCAAACTTACTGCACAAATG+CGG 0.672194 4_1:+25627804 None:intergenic
AGAAAGTGAGCAAAATTGTG+AGG 0.675370 4_1:-25627684 Msa0541640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAAATGTTTTATATTTAT+GGG - chr4_1:25628670-25628689 Msa0541640:CDS 10.0%
!!! AGAAAATGTTTTATATTTAT+GGG - chr4_1:25628670-25628689 Msa0541640:CDS 10.0%
!!! GTTTTAATACTTTTTTTATG+AGG - chr4_1:25627712-25627731 Msa0541640:CDS 15.0%
!!! ATCATCAGTAAATTTATTTT+TGG - chr4_1:25628546-25628565 Msa0541640:intron 15.0%
!!! CAGAAAATGTTTTATATTTA+TGG - chr4_1:25628669-25628688 Msa0541640:CDS 15.0%
!!! GTTTTAATACTTTTTTTATG+AGG - chr4_1:25627712-25627731 Msa0541640:CDS 15.0%
!!! ATCATCAGTAAATTTATTTT+TGG - chr4_1:25628546-25628565 Msa0541640:intron 15.0%
!!! CAGAAAATGTTTTATATTTA+TGG - chr4_1:25628669-25628688 Msa0541640:CDS 15.0%
!! ATTTGTATTTACACTTCAAT+AGG - chr4_1:25627106-25627125 Msa0541640:three_prime_UTR 20.0%
!!! AAAAAATGTTACTATGCTTT+TGG - chr4_1:25627240-25627259 Msa0541640:CDS 20.0%
!!! TTCAAAAGTGGTTTATTTTT+CGG + chr4_1:25627308-25627327 None:intergenic 20.0%
!!! TATAGAGAAATTTTCAAAAG+TGG + chr4_1:25627320-25627339 None:intergenic 20.0%
!! AAATTGAAGTATATAGCATA+GGG - chr4_1:25627418-25627437 Msa0541640:intron 20.0%
!! ACATACACATTAATATGTTA+AGG - chr4_1:25627472-25627491 Msa0541640:intron 20.0%
!!! ACATTAATATGTTAAGGTTT+TGG - chr4_1:25627478-25627497 Msa0541640:intron 20.0%
!!! TGTTTTGTTGTTTTTCTATT+TGG - chr4_1:25627530-25627549 Msa0541640:intron 20.0%
!! ATAAACAACACTTATCATAT+AGG - chr4_1:25627576-25627595 Msa0541640:intron 20.0%
!! TGAAAATAACTTATGAACAA+CGG - chr4_1:25627690-25627709 Msa0541640:CDS 20.0%
!!! AGTGAAAATTTTAATTTGAG+TGG - chr4_1:25627744-25627763 Msa0541640:intron 20.0%
!! AAGTCAAAATGAATTTCAAA+GGG + chr4_1:25627898-25627917 None:intergenic 20.0%
!! AAAGTCAAAATGAATTTCAA+AGG + chr4_1:25627899-25627918 None:intergenic 20.0%
!!! TGAATTTTGTTAAAAGATTC+AGG - chr4_1:25627949-25627968 Msa0541640:intron 20.0%
!!! TGATTGATTCTTAATTAATG+TGG - chr4_1:25628333-25628352 Msa0541640:intron 20.0%
!! AGAATTTAACCTTAATATTG+AGG - chr4_1:25628385-25628404 Msa0541640:intron 20.0%
!!! ATTTTTTTTCCATTGTAACT+TGG - chr4_1:25628615-25628634 Msa0541640:intron 20.0%
!!! TTTTGATCAAGTATAGAATA+TGG - chr4_1:25628782-25628801 Msa0541640:five_prime_UTR 20.0%
!! TTTGATCAAGTATAGAATAT+GGG - chr4_1:25628783-25628802 Msa0541640:five_prime_UTR 20.0%
!! ATTTGTATTTACACTTCAAT+AGG - chr4_1:25627106-25627125 Msa0541640:three_prime_UTR 20.0%
!!! AAAAAATGTTACTATGCTTT+TGG - chr4_1:25627240-25627259 Msa0541640:CDS 20.0%
!!! TTCAAAAGTGGTTTATTTTT+CGG + chr4_1:25627308-25627327 None:intergenic 20.0%
!!! TATAGAGAAATTTTCAAAAG+TGG + chr4_1:25627320-25627339 None:intergenic 20.0%
!! AAATTGAAGTATATAGCATA+GGG - chr4_1:25627418-25627437 Msa0541640:intron 20.0%
!! ACATACACATTAATATGTTA+AGG - chr4_1:25627472-25627491 Msa0541640:intron 20.0%
!!! ACATTAATATGTTAAGGTTT+TGG - chr4_1:25627478-25627497 Msa0541640:intron 20.0%
!!! TGTTTTGTTGTTTTTCTATT+TGG - chr4_1:25627530-25627549 Msa0541640:intron 20.0%
!! ATAAACAACACTTATCATAT+AGG - chr4_1:25627576-25627595 Msa0541640:intron 20.0%
!! TGAAAATAACTTATGAACAA+CGG - chr4_1:25627690-25627709 Msa0541640:CDS 20.0%
!!! AGTGAAAATTTTAATTTGAG+TGG - chr4_1:25627744-25627763 Msa0541640:intron 20.0%
!! AAGTCAAAATGAATTTCAAA+GGG + chr4_1:25627898-25627917 None:intergenic 20.0%
!! AAAGTCAAAATGAATTTCAA+AGG + chr4_1:25627899-25627918 None:intergenic 20.0%
!!! TGAATTTTGTTAAAAGATTC+AGG - chr4_1:25627949-25627968 Msa0541640:intron 20.0%
!!! TGATTGATTCTTAATTAATG+TGG - chr4_1:25628333-25628352 Msa0541640:intron 20.0%
!! AGAATTTAACCTTAATATTG+AGG - chr4_1:25628385-25628404 Msa0541640:intron 20.0%
!!! ATTTTTTTTCCATTGTAACT+TGG - chr4_1:25628615-25628634 Msa0541640:intron 20.0%
!!! TTTTGATCAAGTATAGAATA+TGG - chr4_1:25628782-25628801 Msa0541640:five_prime_UTR 20.0%
!! TTTGATCAAGTATAGAATAT+GGG - chr4_1:25628783-25628802 Msa0541640:five_prime_UTR 20.0%
!! GAACAAAATTGGCTTATTAT+GGG + chr4_1:25626834-25626853 None:intergenic 25.0%
! AATAGTAAGAGATATGGATT+GGG + chr4_1:25626942-25626961 None:intergenic 25.0%
! AGATAGAATAGTAAGAGATA+TGG + chr4_1:25626948-25626967 None:intergenic 25.0%
! ATTTGACATTTGATATGTTG+TGG - chr4_1:25627151-25627170 Msa0541640:three_prime_UTR 25.0%
! AACATTGCATACAATAAATG+TGG + chr4_1:25627207-25627226 None:intergenic 25.0%
! ATTAAATGCATGAAATGACT+TGG - chr4_1:25627270-25627289 Msa0541640:CDS 25.0%
! GAAATTGAAGTATATAGCAT+AGG - chr4_1:25627417-25627436 Msa0541640:intron 25.0%
!!! AAGTGTTGTTTATGCTATAA+AGG + chr4_1:25627569-25627588 None:intergenic 25.0%
!!! AATACTTTTTTTATGAGGTG+AGG - chr4_1:25627717-25627736 Msa0541640:intron 25.0%
! AATAAAAAATTGAACCACGT+AGG - chr4_1:25628165-25628184 Msa0541640:intron 25.0%
!!! ATTATTGGTTTTGGTTTTGA+TGG - chr4_1:25628232-25628251 Msa0541640:intron 25.0%
! CATTATCATCCTCAATATTA+AGG + chr4_1:25628397-25628416 None:intergenic 25.0%
! AAAGAAAGATTAAGCTTAAG+AGG - chr4_1:25628472-25628491 Msa0541640:intron 25.0%
!! TGTTTTCAAAAGAAGTAAGA+GGG - chr4_1:25628817-25628836 Msa0541640:five_prime_UTR 25.0%
!! GAACAAAATTGGCTTATTAT+GGG + chr4_1:25626834-25626853 None:intergenic 25.0%
! AATAGTAAGAGATATGGATT+GGG + chr4_1:25626942-25626961 None:intergenic 25.0%
! AGATAGAATAGTAAGAGATA+TGG + chr4_1:25626948-25626967 None:intergenic 25.0%
! ATTTGACATTTGATATGTTG+TGG - chr4_1:25627151-25627170 Msa0541640:three_prime_UTR 25.0%
! AACATTGCATACAATAAATG+TGG + chr4_1:25627207-25627226 None:intergenic 25.0%
! ATTAAATGCATGAAATGACT+TGG - chr4_1:25627270-25627289 Msa0541640:CDS 25.0%
! GAAATTGAAGTATATAGCAT+AGG - chr4_1:25627417-25627436 Msa0541640:intron 25.0%
!!! AAGTGTTGTTTATGCTATAA+AGG + chr4_1:25627569-25627588 None:intergenic 25.0%
!!! AATACTTTTTTTATGAGGTG+AGG - chr4_1:25627717-25627736 Msa0541640:intron 25.0%
! AATAAAAAATTGAACCACGT+AGG - chr4_1:25628165-25628184 Msa0541640:intron 25.0%
!!! ATTATTGGTTTTGGTTTTGA+TGG - chr4_1:25628232-25628251 Msa0541640:intron 25.0%
! CATTATCATCCTCAATATTA+AGG + chr4_1:25628397-25628416 None:intergenic 25.0%
! AAAGAAAGATTAAGCTTAAG+AGG - chr4_1:25628472-25628491 Msa0541640:intron 25.0%
!! TGTTTTCAAAAGAAGTAAGA+GGG - chr4_1:25628817-25628836 Msa0541640:five_prime_UTR 25.0%
! GGAACAAAATTGGCTTATTA+TGG + chr4_1:25626835-25626854 None:intergenic 30.0%
GAATAGTAAGAGATATGGAT+TGG + chr4_1:25626943-25626962 None:intergenic 30.0%
CTTGTAGAGAAATTGAAAGA+GGG + chr4_1:25627027-25627046 None:intergenic 30.0%
GTAAGTATATGCATAAAGAG+AGG + chr4_1:25627077-25627096 None:intergenic 30.0%
! GAGTGTCCAATTGATAATTT+AGG - chr4_1:25627389-25627408 Msa0541640:intron 30.0%
CCAACACCTAAATTATCAAT+TGG + chr4_1:25627398-25627417 None:intergenic 30.0%
! CCAATTGATAATTTAGGTGT+TGG - chr4_1:25627395-25627414 Msa0541640:intron 30.0%
!! TCTTTCAGAACTTCAAGTTT+TGG - chr4_1:25627787-25627806 Msa0541640:intron 30.0%
AAAACCAATAATGTTCATGC+AGG + chr4_1:25628224-25628243 None:intergenic 30.0%
!!! TGCATGAACATTATTGGTTT+TGG - chr4_1:25628223-25628242 Msa0541640:intron 30.0%
TGATTCTTAATTAATGTGGC+AGG - chr4_1:25628337-25628356 Msa0541640:intron 30.0%
AATACATTGTGAACTGAGTT+AGG - chr4_1:25628520-25628539 Msa0541640:intron 30.0%
AATACGCATAAGCACAAATA+AGG + chr4_1:25628572-25628591 None:intergenic 30.0%
GCAATTTAACCAAGTTACAA+TGG + chr4_1:25628627-25628646 None:intergenic 30.0%
! AACTTTCTAAAAACCGCATT+AGG + chr4_1:25628764-25628783 None:intergenic 30.0%
AAGTATAGAATATGGGTACA+TGG - chr4_1:25628790-25628809 Msa0541640:five_prime_UTR 30.0%
! CTGTTTTCAAAAGAAGTAAG+AGG - chr4_1:25628816-25628835 Msa0541640:five_prime_UTR 30.0%
! GGAACAAAATTGGCTTATTA+TGG + chr4_1:25626835-25626854 None:intergenic 30.0%
GAATAGTAAGAGATATGGAT+TGG + chr4_1:25626943-25626962 None:intergenic 30.0%
CTTGTAGAGAAATTGAAAGA+GGG + chr4_1:25627027-25627046 None:intergenic 30.0%
GTAAGTATATGCATAAAGAG+AGG + chr4_1:25627077-25627096 None:intergenic 30.0%
! GAGTGTCCAATTGATAATTT+AGG - chr4_1:25627389-25627408 Msa0541640:intron 30.0%
CCAACACCTAAATTATCAAT+TGG + chr4_1:25627398-25627417 None:intergenic 30.0%
! CCAATTGATAATTTAGGTGT+TGG - chr4_1:25627395-25627414 Msa0541640:intron 30.0%
!! TCTTTCAGAACTTCAAGTTT+TGG - chr4_1:25627787-25627806 Msa0541640:intron 30.0%
AAAACCAATAATGTTCATGC+AGG + chr4_1:25628224-25628243 None:intergenic 30.0%
!!! TGCATGAACATTATTGGTTT+TGG - chr4_1:25628223-25628242 Msa0541640:intron 30.0%
TGATTCTTAATTAATGTGGC+AGG - chr4_1:25628337-25628356 Msa0541640:intron 30.0%
AATACATTGTGAACTGAGTT+AGG - chr4_1:25628520-25628539 Msa0541640:intron 30.0%
AATACGCATAAGCACAAATA+AGG + chr4_1:25628572-25628591 None:intergenic 30.0%
GCAATTTAACCAAGTTACAA+TGG + chr4_1:25628627-25628646 None:intergenic 30.0%
! AACTTTCTAAAAACCGCATT+AGG + chr4_1:25628764-25628783 None:intergenic 30.0%
AAGTATAGAATATGGGTACA+TGG - chr4_1:25628790-25628809 Msa0541640:five_prime_UTR 30.0%
! CTGTTTTCAAAAGAAGTAAG+AGG - chr4_1:25628816-25628835 Msa0541640:five_prime_UTR 30.0%
AGATAAGTGTGTTATCTAGC+TGG + chr4_1:25626886-25626905 None:intergenic 35.0%
!! TATGGATTGGGTTGATGAAA+CGG + chr4_1:25626930-25626949 None:intergenic 35.0%
CTTCCTAAGCTTCAATACAA+TGG - chr4_1:25626969-25626988 Msa0541640:three_prime_UTR 35.0%
GAAATTGAAAGAGGGTTGAA+AGG + chr4_1:25627019-25627038 None:intergenic 35.0%
CCTTGTAGAGAAATTGAAAG+AGG + chr4_1:25627028-25627047 None:intergenic 35.0%
CCTCTTTCAATTTCTCTACA+AGG - chr4_1:25627025-25627044 Msa0541640:three_prime_UTR 35.0%
GTATATAGCATAGGGTACAT+TGG - chr4_1:25627426-25627445 Msa0541640:intron 35.0%
AGTGGTTAAAACATACATGC+AGG - chr4_1:25627762-25627781 Msa0541640:intron 35.0%
!! AAGTTTTGGTGCAATTTCTG+TGG - chr4_1:25627801-25627820 Msa0541640:intron 35.0%
!! AGTTTTGGTGCAATTTCTGT+GGG - chr4_1:25627802-25627821 Msa0541640:intron 35.0%
TCAAACTTACTGCACAAATG+CGG + chr4_1:25627873-25627892 None:intergenic 35.0%
AGAAAGTGAGCAAAATTGTG+AGG - chr4_1:25627990-25628009 Msa0541640:intron 35.0%
! TTCTGAAAACTTGGTTTCTG+GGG + chr4_1:25628050-25628069 None:intergenic 35.0%
!! GTTCTGAAAACTTGGTTTCT+GGG + chr4_1:25628051-25628070 None:intergenic 35.0%
!! AGTTCTGAAAACTTGGTTTC+TGG + chr4_1:25628052-25628071 None:intergenic 35.0%
! GAAACCAAGTTTTCAGAACT+TGG - chr4_1:25628052-25628071 Msa0541640:intron 35.0%
ATTGAACCACGTAGGTTATA+AGG - chr4_1:25628173-25628192 Msa0541640:intron 35.0%
!!! ATTGGTTTTGGTTTTGATGG+TGG - chr4_1:25628235-25628254 Msa0541640:intron 35.0%
!!! TTTGGTTTTGATGGTGGATT+TGG - chr4_1:25628241-25628260 Msa0541640:intron 35.0%
! TGTTGTTTATGCTAGAGCTA+TGG - chr4_1:25628298-25628317 Msa0541640:intron 35.0%
GTTGTTTATGCTAGAGCTAT+GGG - chr4_1:25628299-25628318 Msa0541640:intron 35.0%
TTCTTAATTAATGTGGCAGG+TGG - chr4_1:25628340-25628359 Msa0541640:intron 35.0%
! TGTGCTTATGCGTATTCTTT+TGG - chr4_1:25628576-25628595 Msa0541640:intron 35.0%
! CTTATGCGTATTCTTTTGGA+AGG - chr4_1:25628580-25628599 Msa0541640:intron 35.0%
!!! TTGCTATTTTGTTGCAAGCA+TGG - chr4_1:25628643-25628662 Msa0541640:CDS 35.0%
!! AGCATGTCATTTGCGTTTTT+AGG - chr4_1:25628708-25628727 Msa0541640:exon 35.0%
!!! GCGTTTTTAGGTCAAGATTA+TGG - chr4_1:25628720-25628739 Msa0541640:five_prime_UTR 35.0%
TTTCAAAAGAAGTAAGAGGG+AGG - chr4_1:25628820-25628839 Msa0541640:five_prime_UTR 35.0%
TTCAAAAGAAGTAAGAGGGA+GGG - chr4_1:25628821-25628840 Msa0541640:five_prime_UTR 35.0%
TTGGCTTATTATGGGATGTA+TGG + chr4_1:25626826-25626845 None:intergenic 35.0%
AGATAAGTGTGTTATCTAGC+TGG + chr4_1:25626886-25626905 None:intergenic 35.0%
!! TATGGATTGGGTTGATGAAA+CGG + chr4_1:25626930-25626949 None:intergenic 35.0%
CTTCCTAAGCTTCAATACAA+TGG - chr4_1:25626969-25626988 Msa0541640:three_prime_UTR 35.0%
GAAATTGAAAGAGGGTTGAA+AGG + chr4_1:25627019-25627038 None:intergenic 35.0%
CCTTGTAGAGAAATTGAAAG+AGG + chr4_1:25627028-25627047 None:intergenic 35.0%
CCTCTTTCAATTTCTCTACA+AGG - chr4_1:25627025-25627044 Msa0541640:three_prime_UTR 35.0%
GTATATAGCATAGGGTACAT+TGG - chr4_1:25627426-25627445 Msa0541640:intron 35.0%
AGTGGTTAAAACATACATGC+AGG - chr4_1:25627762-25627781 Msa0541640:intron 35.0%
!! AAGTTTTGGTGCAATTTCTG+TGG - chr4_1:25627801-25627820 Msa0541640:intron 35.0%
!! AGTTTTGGTGCAATTTCTGT+GGG - chr4_1:25627802-25627821 Msa0541640:intron 35.0%
TCAAACTTACTGCACAAATG+CGG + chr4_1:25627873-25627892 None:intergenic 35.0%
AGAAAGTGAGCAAAATTGTG+AGG - chr4_1:25627990-25628009 Msa0541640:intron 35.0%
! TTCTGAAAACTTGGTTTCTG+GGG + chr4_1:25628050-25628069 None:intergenic 35.0%
!! GTTCTGAAAACTTGGTTTCT+GGG + chr4_1:25628051-25628070 None:intergenic 35.0%
!! AGTTCTGAAAACTTGGTTTC+TGG + chr4_1:25628052-25628071 None:intergenic 35.0%
! GAAACCAAGTTTTCAGAACT+TGG - chr4_1:25628052-25628071 Msa0541640:intron 35.0%
ATTGAACCACGTAGGTTATA+AGG - chr4_1:25628173-25628192 Msa0541640:intron 35.0%
!!! ATTGGTTTTGGTTTTGATGG+TGG - chr4_1:25628235-25628254 Msa0541640:intron 35.0%
!!! TTTGGTTTTGATGGTGGATT+TGG - chr4_1:25628241-25628260 Msa0541640:intron 35.0%
! TGTTGTTTATGCTAGAGCTA+TGG - chr4_1:25628298-25628317 Msa0541640:intron 35.0%
GTTGTTTATGCTAGAGCTAT+GGG - chr4_1:25628299-25628318 Msa0541640:intron 35.0%
TTCTTAATTAATGTGGCAGG+TGG - chr4_1:25628340-25628359 Msa0541640:intron 35.0%
! TGTGCTTATGCGTATTCTTT+TGG - chr4_1:25628576-25628595 Msa0541640:intron 35.0%
! CTTATGCGTATTCTTTTGGA+AGG - chr4_1:25628580-25628599 Msa0541640:intron 35.0%
!!! TTGCTATTTTGTTGCAAGCA+TGG - chr4_1:25628643-25628662 Msa0541640:CDS 35.0%
!! AGCATGTCATTTGCGTTTTT+AGG - chr4_1:25628708-25628727 Msa0541640:exon 35.0%
!!! GCGTTTTTAGGTCAAGATTA+TGG - chr4_1:25628720-25628739 Msa0541640:five_prime_UTR 35.0%
TTTCAAAAGAAGTAAGAGGG+AGG - chr4_1:25628820-25628839 Msa0541640:five_prime_UTR 35.0%
TTCAAAAGAAGTAAGAGGGA+GGG - chr4_1:25628821-25628840 Msa0541640:five_prime_UTR 35.0%
! AGCCAATTTTGTTCCTGACA+TGG - chr4_1:25626840-25626859 Msa0541640:three_prime_UTR 40.0%
AGCCATGTCAGGAACAAAAT+TGG + chr4_1:25626845-25626864 None:intergenic 40.0%
TTGGAAAGTAAAGCCATGTC+AGG + chr4_1:25626856-25626875 None:intergenic 40.0%
TTATCTAGCTGGTGCTTAGT+TGG + chr4_1:25626875-25626894 None:intergenic 40.0%
CGGTAGTAGTAGTAGTAGTA+AGG + chr4_1:25626910-25626929 None:intergenic 40.0%
AGGCCATTGTATTGAAGCTT+AGG + chr4_1:25626975-25626994 None:intergenic 40.0%
TTTCTCTACAAGGACTAACC+AGG - chr4_1:25627035-25627054 Msa0541640:three_prime_UTR 40.0%
GGAAGATGAAAATCTGAACC+TGG + chr4_1:25627056-25627075 None:intergenic 40.0%
ACACGTGCAAATTAAACGCA+TGG - chr4_1:25627358-25627377 Msa0541640:intron 40.0%
AATTAAACGCATGGACGTGA+AGG - chr4_1:25627367-25627386 Msa0541640:intron 40.0%
ATAGCATAGGGTACATTGGT+AGG - chr4_1:25627430-25627449 Msa0541640:intron 40.0%
CGATGAGAAGATCTCAAAGA+AGG + chr4_1:25627847-25627866 None:intergenic 40.0%
!! AGCACCAAGTTCTGAAAACT+TGG + chr4_1:25628059-25628078 None:intergenic 40.0%
AACCACGTAGGTTATAAGGT+CGG - chr4_1:25628177-25628196 Msa0541640:intron 40.0%
GACACCTGCATGAACATTAT+TGG - chr4_1:25628217-25628236 Msa0541640:intron 40.0%
!! GGAAATAGTGATGGGTTTAG+AGG - chr4_1:25628361-25628380 Msa0541640:intron 40.0%
! CAAATCAGCTGCTTGTTGAA+TGG + chr4_1:25628435-25628454 None:intergenic 40.0%
AGCTTAAGAGGTGAACCTAA+TGG - chr4_1:25628484-25628503 Msa0541640:intron 40.0%
TGAACCTAATGGAAGCTCTA+CGG - chr4_1:25628495-25628514 Msa0541640:intron 40.0%
! TTTTCCGTAGAGCTTCCATT+AGG + chr4_1:25628502-25628521 None:intergenic 40.0%
TAAAAACCGCATTAGGCACA+AGG + chr4_1:25628757-25628776 None:intergenic 40.0%
! AGCCAATTTTGTTCCTGACA+TGG - chr4_1:25626840-25626859 Msa0541640:three_prime_UTR 40.0%
AGCCATGTCAGGAACAAAAT+TGG + chr4_1:25626845-25626864 None:intergenic 40.0%
TTGGAAAGTAAAGCCATGTC+AGG + chr4_1:25626856-25626875 None:intergenic 40.0%
TTATCTAGCTGGTGCTTAGT+TGG + chr4_1:25626875-25626894 None:intergenic 40.0%
CGGTAGTAGTAGTAGTAGTA+AGG + chr4_1:25626910-25626929 None:intergenic 40.0%
AGGCCATTGTATTGAAGCTT+AGG + chr4_1:25626975-25626994 None:intergenic 40.0%
TTTCTCTACAAGGACTAACC+AGG - chr4_1:25627035-25627054 Msa0541640:three_prime_UTR 40.0%
GGAAGATGAAAATCTGAACC+TGG + chr4_1:25627056-25627075 None:intergenic 40.0%
ACACGTGCAAATTAAACGCA+TGG - chr4_1:25627358-25627377 Msa0541640:intron 40.0%
AATTAAACGCATGGACGTGA+AGG - chr4_1:25627367-25627386 Msa0541640:intron 40.0%
ATAGCATAGGGTACATTGGT+AGG - chr4_1:25627430-25627449 Msa0541640:intron 40.0%
CGATGAGAAGATCTCAAAGA+AGG + chr4_1:25627847-25627866 None:intergenic 40.0%
!! AGCACCAAGTTCTGAAAACT+TGG + chr4_1:25628059-25628078 None:intergenic 40.0%
AACCACGTAGGTTATAAGGT+CGG - chr4_1:25628177-25628196 Msa0541640:intron 40.0%
GACACCTGCATGAACATTAT+TGG - chr4_1:25628217-25628236 Msa0541640:intron 40.0%
!! GGAAATAGTGATGGGTTTAG+AGG - chr4_1:25628361-25628380 Msa0541640:intron 40.0%
! CAAATCAGCTGCTTGTTGAA+TGG + chr4_1:25628435-25628454 None:intergenic 40.0%
AGCTTAAGAGGTGAACCTAA+TGG - chr4_1:25628484-25628503 Msa0541640:intron 40.0%
TGAACCTAATGGAAGCTCTA+CGG - chr4_1:25628495-25628514 Msa0541640:intron 40.0%
! TTTTCCGTAGAGCTTCCATT+AGG + chr4_1:25628502-25628521 None:intergenic 40.0%
TAAAAACCGCATTAGGCACA+AGG + chr4_1:25628757-25628776 None:intergenic 40.0%
AGAGGGTTGAAAGGTAAGAG+AGG + chr4_1:25627010-25627029 None:intergenic 45.0%
CATTTGATATGTTGTGGCGC+AGG - chr4_1:25627157-25627176 Msa0541640:three_prime_UTR 45.0%
ACCACGTAGGTTATAAGGTC+GGG - chr4_1:25628178-25628197 Msa0541640:intron 45.0%
TGGCAGGTGGAAATAGTGAT+GGG - chr4_1:25628353-25628372 Msa0541640:intron 45.0%
TTCCATCCTTGTGCCTAATG+CGG - chr4_1:25628748-25628767 Msa0541640:five_prime_UTR 45.0%
AAAAGAAGTAAGAGGGAGGG+AGG - chr4_1:25628824-25628843 Msa0541640:five_prime_UTR 45.0%
AGAGGGTTGAAAGGTAAGAG+AGG + chr4_1:25627010-25627029 None:intergenic 45.0%
CATTTGATATGTTGTGGCGC+AGG - chr4_1:25627157-25627176 Msa0541640:three_prime_UTR 45.0%
ACCACGTAGGTTATAAGGTC+GGG - chr4_1:25628178-25628197 Msa0541640:intron 45.0%
TGGCAGGTGGAAATAGTGAT+GGG - chr4_1:25628353-25628372 Msa0541640:intron 45.0%
TTCCATCCTTGTGCCTAATG+CGG - chr4_1:25628748-25628767 Msa0541640:five_prime_UTR 45.0%
AAAAGAAGTAAGAGGGAGGG+AGG - chr4_1:25628824-25628843 Msa0541640:five_prime_UTR 45.0%
AAGAGAGGTGGCTGAAAGAG+AGG + chr4_1:25626995-25627014 None:intergenic 50.0%
GGGTTGAAAGGTAAGAGAGG+TGG + chr4_1:25627007-25627026 None:intergenic 50.0%
GATTCAGGCACAACCTGAGA+CGG - chr4_1:25627964-25627983 Msa0541640:intron 50.0%
! TCTGGGGTAAGCTTGGTTTC+AGG + chr4_1:25628034-25628053 None:intergenic 50.0%
!! CTTGGTTTCTGGGGTAAGCT+TGG + chr4_1:25628041-25628060 None:intergenic 50.0%
ACCCGACCTTATAACCTACG+TGG + chr4_1:25628182-25628201 None:intergenic 50.0%
GTGGCAGGTGGAAATAGTGA+TGG - chr4_1:25628352-25628371 Msa0541640:intron 50.0%
AACCGCATTAGGCACAAGGA+TGG + chr4_1:25628753-25628772 None:intergenic 50.0%
AAGAGAGGTGGCTGAAAGAG+AGG + chr4_1:25626995-25627014 None:intergenic 50.0%
GGGTTGAAAGGTAAGAGAGG+TGG + chr4_1:25627007-25627026 None:intergenic 50.0%
GATTCAGGCACAACCTGAGA+CGG - chr4_1:25627964-25627983 Msa0541640:intron 50.0%
! TCTGGGGTAAGCTTGGTTTC+AGG + chr4_1:25628034-25628053 None:intergenic 50.0%
!! CTTGGTTTCTGGGGTAAGCT+TGG + chr4_1:25628041-25628060 None:intergenic 50.0%
ACCCGACCTTATAACCTACG+TGG + chr4_1:25628182-25628201 None:intergenic 50.0%
GTGGCAGGTGGAAATAGTGA+TGG - chr4_1:25628352-25628371 Msa0541640:intron 50.0%
AACCGCATTAGGCACAAGGA+TGG + chr4_1:25628753-25628772 None:intergenic 50.0%
CTCACTTTCTCCACCGTCTC+AGG + chr4_1:25627980-25627999 None:intergenic 55.0%
GCAGGTGTCAGCTCAGTGAT+AGG + chr4_1:25628206-25628225 None:intergenic 55.0%
CTCACTTTCTCCACCGTCTC+AGG + chr4_1:25627980-25627999 None:intergenic 55.0%
GCAGGTGTCAGCTCAGTGAT+AGG + chr4_1:25628206-25628225 None:intergenic 55.0%
TCAGGCACAACCTGAGACGG+TGG - chr4_1:25627967-25627986 Msa0541640:intron 60.0%
TCAGGCACAACCTGAGACGG+TGG - chr4_1:25627967-25627986 Msa0541640:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 25626824 25628872 25626824 ID=Msa0541640;Name=Msa0541640
chr4_1 mRNA 25626824 25628872 25626824 ID=Msa0541640-mRNA-1;Parent=Msa0541640;Name=Msa0541640-mRNA-1;_AED=0.07;_eAED=0.07;_QI=164|1|1|1|0.66|0.75|4|388|140
chr4_1 exon 25626824 25627337 25626824 ID=Msa0541640-mRNA-1:exon:6911;Parent=Msa0541640-mRNA-1
chr4_1 exon 25627603 25627731 25627603 ID=Msa0541640-mRNA-1:exon:6910;Parent=Msa0541640-mRNA-1
chr4_1 exon 25627814 25627912 25627814 ID=Msa0541640-mRNA-1:exon:6909;Parent=Msa0541640-mRNA-1
chr4_1 exon 25628640 25628872 25628640 ID=Msa0541640-mRNA-1:exon:6908;Parent=Msa0541640-mRNA-1
chr4_1 five_prime_UTR 25628709 25628872 25628709 ID=Msa0541640-mRNA-1:five_prime_utr;Parent=Msa0541640-mRNA-1
chr4_1 CDS 25628640 25628708 25628640 ID=Msa0541640-mRNA-1:cds;Parent=Msa0541640-mRNA-1
chr4_1 CDS 25627814 25627912 25627814 ID=Msa0541640-mRNA-1:cds;Parent=Msa0541640-mRNA-1
chr4_1 CDS 25627603 25627731 25627603 ID=Msa0541640-mRNA-1:cds;Parent=Msa0541640-mRNA-1
chr4_1 CDS 25627212 25627337 25627212 ID=Msa0541640-mRNA-1:cds;Parent=Msa0541640-mRNA-1
chr4_1 three_prime_UTR 25626824 25627211 25626824 ID=Msa0541640-mRNA-1:three_prime_utr;Parent=Msa0541640-mRNA-1
Gene Sequence

>Msa0541640

ATGGCCTCTCTTTCAGCCACCTCTCTTACCTTTCAACCCTCTTTCAATTTCTCTACAAGGACTAACCAGGTTTCTTTCAGAACTTCAAGTTTTGGTGCAATTTCTGTGGGATGTACAAAGAGAAGTTTTCCTTCTTTGAGATCTTCTCATCGTTTCCGCATTTGTGCAATTCAGGCACAACCTGAGACGGTGGAGAAAGTGAGCAAAATTGTGAGGAAACAACTTGCTTTGACTCCTGAAACCAAGCTTACCCCAGAAACCAAGTTTTCAGAACTTGGTGCTGATTCTCTTGACACTGTGGAAATAGTGATGGGTTTAGAGGAAGAATTTAACCTTAATATTGAGGATGATAATGCTGAAGACATCACAACCATTCAACAAGCAGCTGATTTGATAGAGAAACTGATTCAAAAGAAAGATTAA
Protein sequence

>Msa0541640

MASLSATSLTFQPSFNFSTRTNQVSFRTSSFGAISVGCTKRSFPSLRSSHRFRICAIQAQPETVEKVSKIVRKQLALTPETKLTPETKFSELGADSLDTVEIVMGLEEEFNLNIEDDNAEDITTIQQAADLIEKLIQKKD*