AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0550530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550530 MtrunA17_Chr8g0387511 100.000 106 0 0 1 106 1370 1475 1.98e-69 224
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550530 AT4G11270.2 63.208 106 39 0 1 106 1341 1446 6.83e-36 129
Msa0550530 AT4G11270.1 63.208 106 39 0 1 106 1341 1446 6.83e-36 129

Find 74 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTCACTGGGATCTTTC+TGG 0.183894 4_1:+46052882 Msa0550530:CDS
TTGCTCTAATAATATAAATT+AGG 0.219803 4_1:-46054138 None:intergenic
GAAGCAATTGCTTCATAATC+TGG 0.243762 4_1:+46053505 Msa0550530:CDS
AAGCATTATGGCCAGCATTT+TGG 0.247182 4_1:+46053000 Msa0550530:CDS
TTTATTGAGATAAGCAAATA+TGG 0.249757 4_1:+46053794 Msa0550530:three_prime_UTR
TGCTCATACTAGAGTGCTTT+TGG 0.251036 4_1:+46053724 Msa0550530:three_prime_UTR
TTTAAACATTCTTCCCTATA+GGG 0.258407 4_1:+46052821 Msa0550530:five_prime_UTR
CACCACTGGGATTGTCAATT+TGG 0.278393 4_1:+46054025 Msa0550530:three_prime_UTR
TATGATTAGGTGGTGGTCAC+TGG 0.280148 4_1:+46052871 Msa0550530:exon
CTTTAAACATTCTTCCCTAT+AGG 0.291592 4_1:+46052820 Msa0550530:five_prime_UTR
ATGCTTGACCGGGATGAATT+AGG 0.293876 4_1:-46052983 None:intergenic
CGGAACATGGGCTTATGATT+AGG 0.304377 4_1:+46052858 Msa0550530:exon
TATCAGTTTAAGATACTTGT+AGG 0.306397 4_1:-46052779 None:intergenic
TTCATCCCGGTCAAGCATTA+TGG 0.318357 4_1:+46052988 Msa0550530:CDS
TGAAAAGCAGCTTAATTTGC+AGG 0.337493 4_1:+46053027 Msa0550530:CDS
AACTAATATTTGTTCCTCCT+TGG 0.340075 4_1:+46052948 Msa0550530:CDS
AAAAGGCAACCAGCCCCTAT+AGG 0.356362 4_1:-46052835 None:intergenic
ACTAATATTTGTTCCTCCTT+GGG 0.359112 4_1:+46052949 Msa0550530:CDS
TGTAGCGGTGGTGGAGATTT+CGG 0.367408 4_1:+46053609 Msa0550530:exon
TACAAGGCATGGTAATGAAC+TGG 0.379427 4_1:+46053574 Msa0550530:CDS
AAAATTCAACACTACATTTG+AGG 0.385902 4_1:+46053770 Msa0550530:three_prime_UTR
AAAACCATCCAATTGGCTTT+CGG 0.386321 4_1:-46053880 None:intergenic
CTATAGGGAAGAATGTTTAA+AGG 0.402849 4_1:-46052819 None:intergenic
AAAGGCAACCAGCCCCTATA+GGG 0.424466 4_1:-46052834 None:intergenic
TCCACTTAGTGATGCTCCTC+AGG 0.435940 4_1:+46053824 Msa0550530:three_prime_UTR
CCTTGCAATTTCTGAAGTGT+TGG 0.444072 4_1:+46053953 Msa0550530:three_prime_UTR
ACCTCTCTTATCGATTAGAA+TGG 0.447008 4_1:+46053528 Msa0550530:CDS
TGCTGGCCATAATGCTTGAC+CGG 0.457096 4_1:-46052994 None:intergenic
AATACTGACAAAAGAAGAAA+CGG 0.458159 4_1:+46053928 Msa0550530:three_prime_UTR
CCGTCAACATTTGTACAGAC+AGG 0.460052 4_1:+46054210 Msa0550530:three_prime_UTR
GGGCGGAACCGAAAGCCAAT+TGG 0.463624 4_1:+46053872 Msa0550530:three_prime_UTR
ACATTCTTCCCTATAGGGGC+TGG 0.487351 4_1:+46052826 Msa0550530:five_prime_UTR
CCTCTCTTATCGATTAGAAT+GGG 0.490721 4_1:+46053529 Msa0550530:CDS
AATCACTAAAACCATCCAAT+TGG 0.495812 4_1:-46053887 None:intergenic
ATGATTAGGTGGTGGTCACT+GGG 0.496764 4_1:+46052872 Msa0550530:CDS
CATTCAAAATGAGTCACCAC+TGG 0.498930 4_1:+46054011 Msa0550530:three_prime_UTR
CTGTCTGTACAAATGTTGAC+GGG 0.507481 4_1:-46054209 None:intergenic
GGGAAGAATGTTTAAAGGTG+TGG 0.515246 4_1:-46052814 None:intergenic
CATAAGCCCATGTTCCGAAA+AGG 0.523106 4_1:-46052852 None:intergenic
GGAAGAATGTTTAAAGGTGT+GGG 0.524457 4_1:-46052813 None:intergenic
CCTGTCTGTACAAATGTTGA+CGG 0.531317 4_1:-46054210 None:intergenic
TTAAACATTCTTCCCTATAG+GGG 0.536786 4_1:+46052822 Msa0550530:five_prime_UTR
ATATTTGTTCCTCCTTGGGA+GGG 0.537043 4_1:+46052953 Msa0550530:CDS
AATATTTGTTCCTCCTTGGG+AGG 0.543763 4_1:+46052952 Msa0550530:CDS
AAAACTTCAAGAAATGCTAA+AGG 0.548192 4_1:-46054234 None:intergenic
CCAACACTTCAGAAATTGCA+AGG 0.552652 4_1:-46053953 None:intergenic
GATTAGAATGGGTTGCGGAA+CGG 0.559358 4_1:+46053540 Msa0550530:CDS
TGCAAAGGATTCAAATCATG+TGG 0.566355 4_1:+46053475 Msa0550530:CDS
AAAGTACTTCTTACAAGGCA+TGG 0.570990 4_1:+46053563 Msa0550530:CDS
ATACTGACAAAAGAAGAAAC+GGG 0.574237 4_1:+46053929 Msa0550530:three_prime_UTR
TTATCGATTAGAATGGGTTG+CGG 0.577361 4_1:+46053535 Msa0550530:CDS
AACGGAAAGTACTTCTTACA+AGG 0.578988 4_1:+46053558 Msa0550530:CDS
TGAGCTGCTATACATGCTAA+AGG 0.581954 4_1:-46053671 None:intergenic
CGAAGTCTCAATGTGTGCAT+CGG 0.582714 4_1:-46054282 None:intergenic
ACTGGACAGGAACAAAGTTC+CGG 0.587058 4_1:-46052919 None:intergenic
TCCTGAGGAGCATCACTAAG+TGG 0.591189 4_1:-46053825 None:intergenic
TTGTAGGAAACATCAATCCC+AGG 0.599209 4_1:-46052763 None:intergenic
CGTCAACATTTGTACAGACA+GGG 0.606704 4_1:+46054211 Msa0550530:three_prime_UTR
ATTCAAAATGAGTCACCACT+GGG 0.615033 4_1:+46054012 Msa0550530:three_prime_UTR
ACAAGGCATGGTAATGAACT+GGG 0.621865 4_1:+46053575 Msa0550530:CDS
TCTGGTATGAGAAACTGAGC+CGG 0.626357 4_1:+46052900 Msa0550530:CDS
GGAACCGAAAGCCAATTGGA+TGG 0.627615 4_1:+46053876 Msa0550530:three_prime_UTR
TGTTGGCAAACTAATTGTGA+TGG 0.631625 4_1:+46053970 Msa0550530:three_prime_UTR
AACATGGGCTTATGATTAGG+TGG 0.634033 4_1:+46052861 Msa0550530:exon
ATGGGCTTATGATTAGGTGG+TGG 0.637208 4_1:+46052864 Msa0550530:exon
AGAATGTTTAAAGGTGTGGG+AGG 0.649073 4_1:-46052810 None:intergenic
GATTCTCACCTAATTCATCC+CGG 0.651461 4_1:+46052975 Msa0550530:CDS
GCTGGCCATAATGCTTGACC+GGG 0.658543 4_1:-46052993 None:intergenic
GAATGTTTAAAGGTGTGGGA+GGG 0.663074 4_1:-46052809 None:intergenic
TTAGGTGAGAATCCCTCCCA+AGG 0.668091 4_1:-46052965 None:intergenic
CCCATTCTAATCGATAAGAG+AGG 0.695297 4_1:-46053529 None:intergenic
GGTGAGAATCCCTCCCAAGG+AGG 0.708180 4_1:-46052962 None:intergenic
TGTCTGTACAAATGTTGACG+GGG 0.711757 4_1:-46054208 None:intergenic
GACCAAATTGACAATCCCAG+TGG 0.738687 4_1:-46054027 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAATGATAAATAGATTTA+AGG - chr4_1:46053421-46053440 None:intergenic 15.0%
!! TTGCTCTAATAATATAAATT+AGG - chr4_1:46054141-46054160 None:intergenic 15.0%
!! GAAAATGATAAATAGATTTA+AGG - chr4_1:46053421-46053440 None:intergenic 15.0%
!! TTGCTCTAATAATATAAATT+AGG - chr4_1:46054141-46054160 None:intergenic 15.0%
!! CATACTAAAATACATTGAAT+TGG + chr4_1:46053277-46053296 Msa0550530:intron 20.0%
!! AAATAGATTTAAGGAAATAG+TGG - chr4_1:46053412-46053431 None:intergenic 20.0%
!! TATTCTACAAAAGGAAATAT+TGG - chr4_1:46053454-46053473 None:intergenic 20.0%
!! TTTATTGAGATAAGCAAATA+TGG + chr4_1:46053794-46053813 Msa0550530:three_prime_UTR 20.0%
!!! CTTTAAAAAAGGAATGTTTT+GGG + chr4_1:46053852-46053871 Msa0550530:three_prime_UTR 20.0%
!!! TGAATGATTTTGTGATTATT+TGG - chr4_1:46053997-46054016 None:intergenic 20.0%
!!! AAATATTTTCTCGAGAATAA+CGG + chr4_1:46054068-46054087 Msa0550530:three_prime_UTR 20.0%
!! CATACTAAAATACATTGAAT+TGG + chr4_1:46053277-46053296 Msa0550530:intron 20.0%
!! AAATAGATTTAAGGAAATAG+TGG - chr4_1:46053412-46053431 None:intergenic 20.0%
!! TATTCTACAAAAGGAAATAT+TGG - chr4_1:46053454-46053473 None:intergenic 20.0%
!! TTTATTGAGATAAGCAAATA+TGG + chr4_1:46053794-46053813 Msa0550530:three_prime_UTR 20.0%
!!! CTTTAAAAAAGGAATGTTTT+GGG + chr4_1:46053852-46053871 Msa0550530:three_prime_UTR 20.0%
!!! TGAATGATTTTGTGATTATT+TGG - chr4_1:46053997-46054016 None:intergenic 20.0%
!!! AAATATTTTCTCGAGAATAA+CGG + chr4_1:46054068-46054087 Msa0550530:three_prime_UTR 20.0%
!! TTACTGTGAAATTATTTTCC+TGG + chr4_1:46052745-46052764 Msa0550530:five_prime_UTR 25.0%
!! TACTGTGAAATTATTTTCCT+GGG + chr4_1:46052746-46052765 Msa0550530:five_prime_UTR 25.0%
!! TATCAGTTTAAGATACTTGT+AGG - chr4_1:46052782-46052801 None:intergenic 25.0%
! TTTAAACATTCTTCCCTATA+GGG + chr4_1:46052821-46052840 Msa0550530:five_prime_UTR 25.0%
! ACCGAGTTTAAATGAAAAAA+GGG - chr4_1:46053216-46053235 None:intergenic 25.0%
! TACCGAGTTTAAATGAAAAA+AGG - chr4_1:46053217-46053236 None:intergenic 25.0%
! CTTTCAATCAACTTTCTATT+AGG - chr4_1:46053257-46053276 None:intergenic 25.0%
! AAAATTCAACACTACATTTG+AGG + chr4_1:46053770-46053789 Msa0550530:three_prime_UTR 25.0%
!!! CCAAAACATTCCTTTTTTAA+AGG - chr4_1:46053854-46053873 None:intergenic 25.0%
!!! CCTTTAAAAAAGGAATGTTT+TGG + chr4_1:46053851-46053870 Msa0550530:three_prime_UTR 25.0%
! AATACTGACAAAAGAAGAAA+CGG + chr4_1:46053928-46053947 Msa0550530:three_prime_UTR 25.0%
!! AATATTTTCTCGAGAATAAC+GGG + chr4_1:46054069-46054088 Msa0550530:three_prime_UTR 25.0%
! AAAACTTCAAGAAATGCTAA+AGG - chr4_1:46054237-46054256 None:intergenic 25.0%
!! TTACTGTGAAATTATTTTCC+TGG + chr4_1:46052745-46052764 Msa0550530:five_prime_UTR 25.0%
!! TACTGTGAAATTATTTTCCT+GGG + chr4_1:46052746-46052765 Msa0550530:five_prime_UTR 25.0%
!! TATCAGTTTAAGATACTTGT+AGG - chr4_1:46052782-46052801 None:intergenic 25.0%
! TTTAAACATTCTTCCCTATA+GGG + chr4_1:46052821-46052840 Msa0550530:five_prime_UTR 25.0%
! ACCGAGTTTAAATGAAAAAA+GGG - chr4_1:46053216-46053235 None:intergenic 25.0%
! TACCGAGTTTAAATGAAAAA+AGG - chr4_1:46053217-46053236 None:intergenic 25.0%
! CTTTCAATCAACTTTCTATT+AGG - chr4_1:46053257-46053276 None:intergenic 25.0%
! AAAATTCAACACTACATTTG+AGG + chr4_1:46053770-46053789 Msa0550530:three_prime_UTR 25.0%
!!! CCAAAACATTCCTTTTTTAA+AGG - chr4_1:46053854-46053873 None:intergenic 25.0%
!!! CCTTTAAAAAAGGAATGTTT+TGG + chr4_1:46053851-46053870 Msa0550530:three_prime_UTR 25.0%
! AATACTGACAAAAGAAGAAA+CGG + chr4_1:46053928-46053947 Msa0550530:three_prime_UTR 25.0%
!! AATATTTTCTCGAGAATAAC+GGG + chr4_1:46054069-46054088 Msa0550530:three_prime_UTR 25.0%
! AAAACTTCAAGAAATGCTAA+AGG - chr4_1:46054237-46054256 None:intergenic 25.0%
CTATAGGGAAGAATGTTTAA+AGG - chr4_1:46052822-46052841 None:intergenic 30.0%
CTTTAAACATTCTTCCCTAT+AGG + chr4_1:46052820-46052839 Msa0550530:five_prime_UTR 30.0%
TTAAACATTCTTCCCTATAG+GGG + chr4_1:46052822-46052841 Msa0550530:five_prime_UTR 30.0%
!!! CAAATATTAGTTTTGTGCAC+TGG - chr4_1:46052940-46052959 None:intergenic 30.0%
AACTAATATTTGTTCCTCCT+TGG + chr4_1:46052948-46052967 Msa0550530:CDS 30.0%
ACTAATATTTGTTCCTCCTT+GGG + chr4_1:46052949-46052968 Msa0550530:CDS 30.0%
!!! GCCCTTTTTTCATTTAAACT+CGG + chr4_1:46053212-46053231 Msa0550530:intron 30.0%
!!! GTACTACTTTTTACCATCAT+TGG + chr4_1:46053368-46053387 Msa0550530:intron 30.0%
! AGGAAATATTGGTGGTATTA+CGG - chr4_1:46053443-46053462 None:intergenic 30.0%
TCTACAAAAGGAAATATTGG+TGG - chr4_1:46053451-46053470 None:intergenic 30.0%
CCTTTGCATTATTCTACAAA+AGG - chr4_1:46053463-46053482 None:intergenic 30.0%
! CCTTTTGTAGAATAATGCAA+AGG + chr4_1:46053460-46053479 Msa0550530:intron 30.0%
!!! TGATTTCACAGTTTTAGTTG+AGG + chr4_1:46053632-46053651 Msa0550530:three_prime_UTR 30.0%
!! TAAAAAAGGAATGTTTTGGG+CGG + chr4_1:46053855-46053874 Msa0550530:three_prime_UTR 30.0%
AATCACTAAAACCATCCAAT+TGG - chr4_1:46053890-46053909 None:intergenic 30.0%
!!! ATTGGATGGTTTTAGTGATT+TGG + chr4_1:46053890-46053909 Msa0550530:three_prime_UTR 30.0%
ATACTGACAAAAGAAGAAAC+GGG + chr4_1:46053929-46053948 Msa0550530:three_prime_UTR 30.0%
! ATATTTTCTCGAGAATAACG+GGG + chr4_1:46054070-46054089 Msa0550530:three_prime_UTR 30.0%
CTATAGGGAAGAATGTTTAA+AGG - chr4_1:46052822-46052841 None:intergenic 30.0%
CTTTAAACATTCTTCCCTAT+AGG + chr4_1:46052820-46052839 Msa0550530:five_prime_UTR 30.0%
TTAAACATTCTTCCCTATAG+GGG + chr4_1:46052822-46052841 Msa0550530:five_prime_UTR 30.0%
!!! CAAATATTAGTTTTGTGCAC+TGG - chr4_1:46052940-46052959 None:intergenic 30.0%
AACTAATATTTGTTCCTCCT+TGG + chr4_1:46052948-46052967 Msa0550530:CDS 30.0%
ACTAATATTTGTTCCTCCTT+GGG + chr4_1:46052949-46052968 Msa0550530:CDS 30.0%
!!! GCCCTTTTTTCATTTAAACT+CGG + chr4_1:46053212-46053231 Msa0550530:intron 30.0%
!!! GTACTACTTTTTACCATCAT+TGG + chr4_1:46053368-46053387 Msa0550530:intron 30.0%
! AGGAAATATTGGTGGTATTA+CGG - chr4_1:46053443-46053462 None:intergenic 30.0%
TCTACAAAAGGAAATATTGG+TGG - chr4_1:46053451-46053470 None:intergenic 30.0%
CCTTTGCATTATTCTACAAA+AGG - chr4_1:46053463-46053482 None:intergenic 30.0%
! CCTTTTGTAGAATAATGCAA+AGG + chr4_1:46053460-46053479 Msa0550530:intron 30.0%
!!! TGATTTCACAGTTTTAGTTG+AGG + chr4_1:46053632-46053651 Msa0550530:three_prime_UTR 30.0%
!! TAAAAAAGGAATGTTTTGGG+CGG + chr4_1:46053855-46053874 Msa0550530:three_prime_UTR 30.0%
AATCACTAAAACCATCCAAT+TGG - chr4_1:46053890-46053909 None:intergenic 30.0%
!!! ATTGGATGGTTTTAGTGATT+TGG + chr4_1:46053890-46053909 Msa0550530:three_prime_UTR 30.0%
ATACTGACAAAAGAAGAAAC+GGG + chr4_1:46053929-46053948 Msa0550530:three_prime_UTR 30.0%
! ATATTTTCTCGAGAATAACG+GGG + chr4_1:46054070-46054089 Msa0550530:three_prime_UTR 30.0%
! GGAAGAATGTTTAAAGGTGT+GGG - chr4_1:46052816-46052835 None:intergenic 35.0%
! CTTTTCAGTATCCAAAATGC+TGG - chr4_1:46053014-46053033 None:intergenic 35.0%
!! TGAAAAGCAGCTTAATTTGC+AGG + chr4_1:46053027-46053046 Msa0550530:CDS 35.0%
TAACGAGTGAAACTAGAACT+TGG + chr4_1:46053101-46053120 Msa0550530:intron 35.0%
!!! CTACTTTTTACCATCATTGG+TGG + chr4_1:46053371-46053390 Msa0550530:intron 35.0%
TGCAAAGGATTCAAATCATG+TGG + chr4_1:46053475-46053494 Msa0550530:CDS 35.0%
GAAGCAATTGCTTCATAATC+TGG + chr4_1:46053505-46053524 Msa0550530:CDS 35.0%
! ACCTCTCTTATCGATTAGAA+TGG + chr4_1:46053528-46053547 Msa0550530:CDS 35.0%
! CCTCTCTTATCGATTAGAAT+GGG + chr4_1:46053529-46053548 Msa0550530:CDS 35.0%
!! TTATCGATTAGAATGGGTTG+CGG + chr4_1:46053535-46053554 Msa0550530:CDS 35.0%
!! AACGGAAAGTACTTCTTACA+AGG + chr4_1:46053558-46053577 Msa0550530:CDS 35.0%
! AAAGTACTTCTTACAAGGCA+TGG + chr4_1:46053563-46053582 Msa0550530:CDS 35.0%
!!! CTTTTTTAAAGGACTTCCTG+AGG - chr4_1:46053843-46053862 None:intergenic 35.0%
! CTCAGGAAGTCCTTTAAAAA+AGG + chr4_1:46053841-46053860 Msa0550530:three_prime_UTR 35.0%
!! AAAACCATCCAATTGGCTTT+CGG - chr4_1:46053883-46053902 None:intergenic 35.0%
TGTTGGCAAACTAATTGTGA+TGG + chr4_1:46053970-46053989 Msa0550530:three_prime_UTR 35.0%
ATTCAAAATGAGTCACCACT+GGG + chr4_1:46054012-46054031 Msa0550530:three_prime_UTR 35.0%
! GGAAGAATGTTTAAAGGTGT+GGG - chr4_1:46052816-46052835 None:intergenic 35.0%
! CTTTTCAGTATCCAAAATGC+TGG - chr4_1:46053014-46053033 None:intergenic 35.0%
!! TGAAAAGCAGCTTAATTTGC+AGG + chr4_1:46053027-46053046 Msa0550530:CDS 35.0%
TAACGAGTGAAACTAGAACT+TGG + chr4_1:46053101-46053120 Msa0550530:intron 35.0%
!!! CTACTTTTTACCATCATTGG+TGG + chr4_1:46053371-46053390 Msa0550530:intron 35.0%
TGCAAAGGATTCAAATCATG+TGG + chr4_1:46053475-46053494 Msa0550530:CDS 35.0%
GAAGCAATTGCTTCATAATC+TGG + chr4_1:46053505-46053524 Msa0550530:CDS 35.0%
! ACCTCTCTTATCGATTAGAA+TGG + chr4_1:46053528-46053547 Msa0550530:CDS 35.0%
! CCTCTCTTATCGATTAGAAT+GGG + chr4_1:46053529-46053548 Msa0550530:CDS 35.0%
!! TTATCGATTAGAATGGGTTG+CGG + chr4_1:46053535-46053554 Msa0550530:CDS 35.0%
!! AACGGAAAGTACTTCTTACA+AGG + chr4_1:46053558-46053577 Msa0550530:CDS 35.0%
! AAAGTACTTCTTACAAGGCA+TGG + chr4_1:46053563-46053582 Msa0550530:CDS 35.0%
!!! CTTTTTTAAAGGACTTCCTG+AGG - chr4_1:46053843-46053862 None:intergenic 35.0%
! CTCAGGAAGTCCTTTAAAAA+AGG + chr4_1:46053841-46053860 Msa0550530:three_prime_UTR 35.0%
!! AAAACCATCCAATTGGCTTT+CGG - chr4_1:46053883-46053902 None:intergenic 35.0%
TGTTGGCAAACTAATTGTGA+TGG + chr4_1:46053970-46053989 Msa0550530:three_prime_UTR 35.0%
ATTCAAAATGAGTCACCACT+GGG + chr4_1:46054012-46054031 Msa0550530:three_prime_UTR 35.0%
TTGTAGGAAACATCAATCCC+AGG - chr4_1:46052766-46052785 None:intergenic 40.0%
! GAATGTTTAAAGGTGTGGGA+GGG - chr4_1:46052812-46052831 None:intergenic 40.0%
! AGAATGTTTAAAGGTGTGGG+AGG - chr4_1:46052813-46052832 None:intergenic 40.0%
GGGAAGAATGTTTAAAGGTG+TGG - chr4_1:46052817-46052836 None:intergenic 40.0%
AACATGGGCTTATGATTAGG+TGG + chr4_1:46052861-46052880 Msa0550530:exon 40.0%
!!! ATTAGTTTTGTGCACTGGAC+AGG - chr4_1:46052935-46052954 None:intergenic 40.0%
AATATTTGTTCCTCCTTGGG+AGG + chr4_1:46052952-46052971 Msa0550530:CDS 40.0%
ATATTTGTTCCTCCTTGGGA+GGG + chr4_1:46052953-46052972 Msa0550530:CDS 40.0%
GATTCTCACCTAATTCATCC+CGG + chr4_1:46052975-46052994 Msa0550530:CDS 40.0%
! AAGCATTATGGCCAGCATTT+TGG + chr4_1:46053000-46053019 Msa0550530:CDS 40.0%
AAGGGCTTTCAACTTAGCAA+TGG - chr4_1:46053198-46053217 None:intergenic 40.0%
CTCAAGCTATCTAGATCATG+TGG - chr4_1:46053339-46053358 None:intergenic 40.0%
CCCATTCTAATCGATAAGAG+AGG - chr4_1:46053532-46053551 None:intergenic 40.0%
! TACAAGGCATGGTAATGAAC+TGG + chr4_1:46053574-46053593 Msa0550530:CDS 40.0%
! ACAAGGCATGGTAATGAACT+GGG + chr4_1:46053575-46053594 Msa0550530:CDS 40.0%
TGAGCTGCTATACATGCTAA+AGG - chr4_1:46053674-46053693 None:intergenic 40.0%
!! TGCTCATACTAGAGTGCTTT+TGG + chr4_1:46053724-46053743 Msa0550530:three_prime_UTR 40.0%
!! TCATACTAGAGTGCTTTTGG+AGG + chr4_1:46053727-46053746 Msa0550530:three_prime_UTR 40.0%
CCAACACTTCAGAAATTGCA+AGG - chr4_1:46053956-46053975 None:intergenic 40.0%
!! CCTTGCAATTTCTGAAGTGT+TGG + chr4_1:46053953-46053972 Msa0550530:three_prime_UTR 40.0%
CATTCAAAATGAGTCACCAC+TGG + chr4_1:46054011-46054030 Msa0550530:three_prime_UTR 40.0%
!!! AGTTTTGATAGTGAGCAGAG+AGG + chr4_1:46054099-46054118 Msa0550530:three_prime_UTR 40.0%
TGTCTGTACAAATGTTGACG+GGG - chr4_1:46054211-46054230 None:intergenic 40.0%
CTGTCTGTACAAATGTTGAC+GGG - chr4_1:46054212-46054231 None:intergenic 40.0%
CCTGTCTGTACAAATGTTGA+CGG - chr4_1:46054213-46054232 None:intergenic 40.0%
CGTCAACATTTGTACAGACA+GGG + chr4_1:46054211-46054230 Msa0550530:three_prime_UTR 40.0%
TTGTAGGAAACATCAATCCC+AGG - chr4_1:46052766-46052785 None:intergenic 40.0%
! GAATGTTTAAAGGTGTGGGA+GGG - chr4_1:46052812-46052831 None:intergenic 40.0%
! AGAATGTTTAAAGGTGTGGG+AGG - chr4_1:46052813-46052832 None:intergenic 40.0%
GGGAAGAATGTTTAAAGGTG+TGG - chr4_1:46052817-46052836 None:intergenic 40.0%
AACATGGGCTTATGATTAGG+TGG + chr4_1:46052861-46052880 Msa0550530:exon 40.0%
!!! ATTAGTTTTGTGCACTGGAC+AGG - chr4_1:46052935-46052954 None:intergenic 40.0%
AATATTTGTTCCTCCTTGGG+AGG + chr4_1:46052952-46052971 Msa0550530:CDS 40.0%
ATATTTGTTCCTCCTTGGGA+GGG + chr4_1:46052953-46052972 Msa0550530:CDS 40.0%
GATTCTCACCTAATTCATCC+CGG + chr4_1:46052975-46052994 Msa0550530:CDS 40.0%
! AAGCATTATGGCCAGCATTT+TGG + chr4_1:46053000-46053019 Msa0550530:CDS 40.0%
AAGGGCTTTCAACTTAGCAA+TGG - chr4_1:46053198-46053217 None:intergenic 40.0%
CTCAAGCTATCTAGATCATG+TGG - chr4_1:46053339-46053358 None:intergenic 40.0%
CCCATTCTAATCGATAAGAG+AGG - chr4_1:46053532-46053551 None:intergenic 40.0%
! TACAAGGCATGGTAATGAAC+TGG + chr4_1:46053574-46053593 Msa0550530:CDS 40.0%
! ACAAGGCATGGTAATGAACT+GGG + chr4_1:46053575-46053594 Msa0550530:CDS 40.0%
TGAGCTGCTATACATGCTAA+AGG - chr4_1:46053674-46053693 None:intergenic 40.0%
!! TGCTCATACTAGAGTGCTTT+TGG + chr4_1:46053724-46053743 Msa0550530:three_prime_UTR 40.0%
!! TCATACTAGAGTGCTTTTGG+AGG + chr4_1:46053727-46053746 Msa0550530:three_prime_UTR 40.0%
CCAACACTTCAGAAATTGCA+AGG - chr4_1:46053956-46053975 None:intergenic 40.0%
!! CCTTGCAATTTCTGAAGTGT+TGG + chr4_1:46053953-46053972 Msa0550530:three_prime_UTR 40.0%
CATTCAAAATGAGTCACCAC+TGG + chr4_1:46054011-46054030 Msa0550530:three_prime_UTR 40.0%
!!! AGTTTTGATAGTGAGCAGAG+AGG + chr4_1:46054099-46054118 Msa0550530:three_prime_UTR 40.0%
TGTCTGTACAAATGTTGACG+GGG - chr4_1:46054211-46054230 None:intergenic 40.0%
CTGTCTGTACAAATGTTGAC+GGG - chr4_1:46054212-46054231 None:intergenic 40.0%
CCTGTCTGTACAAATGTTGA+CGG - chr4_1:46054213-46054232 None:intergenic 40.0%
CGTCAACATTTGTACAGACA+GGG + chr4_1:46054211-46054230 Msa0550530:three_prime_UTR 40.0%
! TGGTTGCCTTTTCGGAACAT+GGG + chr4_1:46052846-46052865 Msa0550530:five_prime_UTR 45.0%
CATAAGCCCATGTTCCGAAA+AGG - chr4_1:46052855-46052874 None:intergenic 45.0%
CGGAACATGGGCTTATGATT+AGG + chr4_1:46052858-46052877 Msa0550530:exon 45.0%
ATGGGCTTATGATTAGGTGG+TGG + chr4_1:46052864-46052883 Msa0550530:exon 45.0%
! TATGATTAGGTGGTGGTCAC+TGG + chr4_1:46052871-46052890 Msa0550530:exon 45.0%
! ATGATTAGGTGGTGGTCACT+GGG + chr4_1:46052872-46052891 Msa0550530:CDS 45.0%
TCTGGTATGAGAAACTGAGC+CGG + chr4_1:46052900-46052919 Msa0550530:CDS 45.0%
ACTGGACAGGAACAAAGTTC+CGG - chr4_1:46052922-46052941 None:intergenic 45.0%
ATGCTTGACCGGGATGAATT+AGG - chr4_1:46052986-46053005 None:intergenic 45.0%
TTCATCCCGGTCAAGCATTA+TGG + chr4_1:46052988-46053007 Msa0550530:CDS 45.0%
!! CTAGAACTTGGTCTTTTGCC+AGG + chr4_1:46053113-46053132 Msa0550530:intron 45.0%
! AACTCGTTGCATTTTCCCTC+AGG - chr4_1:46053147-46053166 None:intergenic 45.0%
CTATCTAGATCATGTGGCAG+TGG - chr4_1:46053333-46053352 None:intergenic 45.0%
! GACTTGATGACCACCAATGA+TGG - chr4_1:46053384-46053403 None:intergenic 45.0%
!! GATTAGAATGGGTTGCGGAA+CGG + chr4_1:46053540-46053559 Msa0550530:CDS 45.0%
! TGGGCACTTTTCAGTTGTAG+CGG + chr4_1:46053594-46053613 Msa0550530:CDS 45.0%
CACCACTGGGATTGTCAATT+TGG + chr4_1:46054025-46054044 Msa0550530:three_prime_UTR 45.0%
GACCAAATTGACAATCCCAG+TGG - chr4_1:46054030-46054049 None:intergenic 45.0%
CCGTCAACATTTGTACAGAC+AGG + chr4_1:46054210-46054229 Msa0550530:three_prime_UTR 45.0%
CGAAGTCTCAATGTGTGCAT+CGG - chr4_1:46054285-46054304 None:intergenic 45.0%
! TGGTTGCCTTTTCGGAACAT+GGG + chr4_1:46052846-46052865 Msa0550530:five_prime_UTR 45.0%
CATAAGCCCATGTTCCGAAA+AGG - chr4_1:46052855-46052874 None:intergenic 45.0%
CGGAACATGGGCTTATGATT+AGG + chr4_1:46052858-46052877 Msa0550530:exon 45.0%
ATGGGCTTATGATTAGGTGG+TGG + chr4_1:46052864-46052883 Msa0550530:exon 45.0%
! TATGATTAGGTGGTGGTCAC+TGG + chr4_1:46052871-46052890 Msa0550530:exon 45.0%
! ATGATTAGGTGGTGGTCACT+GGG + chr4_1:46052872-46052891 Msa0550530:CDS 45.0%
TCTGGTATGAGAAACTGAGC+CGG + chr4_1:46052900-46052919 Msa0550530:CDS 45.0%
ACTGGACAGGAACAAAGTTC+CGG - chr4_1:46052922-46052941 None:intergenic 45.0%
ATGCTTGACCGGGATGAATT+AGG - chr4_1:46052986-46053005 None:intergenic 45.0%
TTCATCCCGGTCAAGCATTA+TGG + chr4_1:46052988-46053007 Msa0550530:CDS 45.0%
!! CTAGAACTTGGTCTTTTGCC+AGG + chr4_1:46053113-46053132 Msa0550530:intron 45.0%
! AACTCGTTGCATTTTCCCTC+AGG - chr4_1:46053147-46053166 None:intergenic 45.0%
CTATCTAGATCATGTGGCAG+TGG - chr4_1:46053333-46053352 None:intergenic 45.0%
! GACTTGATGACCACCAATGA+TGG - chr4_1:46053384-46053403 None:intergenic 45.0%
!! GATTAGAATGGGTTGCGGAA+CGG + chr4_1:46053540-46053559 Msa0550530:CDS 45.0%
! TGGGCACTTTTCAGTTGTAG+CGG + chr4_1:46053594-46053613 Msa0550530:CDS 45.0%
CACCACTGGGATTGTCAATT+TGG + chr4_1:46054025-46054044 Msa0550530:three_prime_UTR 45.0%
GACCAAATTGACAATCCCAG+TGG - chr4_1:46054030-46054049 None:intergenic 45.0%
CCGTCAACATTTGTACAGAC+AGG + chr4_1:46054210-46054229 Msa0550530:three_prime_UTR 45.0%
CGAAGTCTCAATGTGTGCAT+CGG - chr4_1:46054285-46054304 None:intergenic 45.0%
ACATTCTTCCCTATAGGGGC+TGG + chr4_1:46052826-46052845 Msa0550530:five_prime_UTR 50.0%
AAAGGCAACCAGCCCCTATA+GGG - chr4_1:46052837-46052856 None:intergenic 50.0%
AAAAGGCAACCAGCCCCTAT+AGG - chr4_1:46052838-46052857 None:intergenic 50.0%
!!! ATAGGGGCTGGTTGCCTTTT+CGG + chr4_1:46052838-46052857 Msa0550530:five_prime_UTR 50.0%
! CTGGTTGCCTTTTCGGAACA+TGG + chr4_1:46052845-46052864 Msa0550530:five_prime_UTR 50.0%
TTAGGTGAGAATCCCTCCCA+AGG - chr4_1:46052968-46052987 None:intergenic 50.0%
TGCTGGCCATAATGCTTGAC+CGG - chr4_1:46052997-46053016 None:intergenic 50.0%
!! CTTGGTCTTTTGCCAGGTAC+TGG + chr4_1:46053119-46053138 Msa0550530:intron 50.0%
! GCACTTTTCAGTTGTAGCGG+TGG + chr4_1:46053597-46053616 Msa0550530:exon 50.0%
! CTTTTCAGTTGTAGCGGTGG+TGG + chr4_1:46053600-46053619 Msa0550530:exon 50.0%
!! TGTAGCGGTGGTGGAGATTT+CGG + chr4_1:46053609-46053628 Msa0550530:exon 50.0%
TCCACTTAGTGATGCTCCTC+AGG + chr4_1:46053824-46053843 Msa0550530:three_prime_UTR 50.0%
!! TCCTGAGGAGCATCACTAAG+TGG - chr4_1:46053828-46053847 None:intergenic 50.0%
GGAACCGAAAGCCAATTGGA+TGG + chr4_1:46053876-46053895 Msa0550530:three_prime_UTR 50.0%
ACATTCTTCCCTATAGGGGC+TGG + chr4_1:46052826-46052845 Msa0550530:five_prime_UTR 50.0%
AAAGGCAACCAGCCCCTATA+GGG - chr4_1:46052837-46052856 None:intergenic 50.0%
AAAAGGCAACCAGCCCCTAT+AGG - chr4_1:46052838-46052857 None:intergenic 50.0%
!!! ATAGGGGCTGGTTGCCTTTT+CGG + chr4_1:46052838-46052857 Msa0550530:five_prime_UTR 50.0%
! CTGGTTGCCTTTTCGGAACA+TGG + chr4_1:46052845-46052864 Msa0550530:five_prime_UTR 50.0%
TTAGGTGAGAATCCCTCCCA+AGG - chr4_1:46052968-46052987 None:intergenic 50.0%
TGCTGGCCATAATGCTTGAC+CGG - chr4_1:46052997-46053016 None:intergenic 50.0%
!! CTTGGTCTTTTGCCAGGTAC+TGG + chr4_1:46053119-46053138 Msa0550530:intron 50.0%
! GCACTTTTCAGTTGTAGCGG+TGG + chr4_1:46053597-46053616 Msa0550530:exon 50.0%
! CTTTTCAGTTGTAGCGGTGG+TGG + chr4_1:46053600-46053619 Msa0550530:exon 50.0%
!! TGTAGCGGTGGTGGAGATTT+CGG + chr4_1:46053609-46053628 Msa0550530:exon 50.0%
TCCACTTAGTGATGCTCCTC+AGG + chr4_1:46053824-46053843 Msa0550530:three_prime_UTR 50.0%
!! TCCTGAGGAGCATCACTAAG+TGG - chr4_1:46053828-46053847 None:intergenic 50.0%
GGAACCGAAAGCCAATTGGA+TGG + chr4_1:46053876-46053895 Msa0550530:three_prime_UTR 50.0%
GGTGGTCACTGGGATCTTTC+TGG + chr4_1:46052882-46052901 Msa0550530:CDS 55.0%
GCTGGCCATAATGCTTGACC+GGG - chr4_1:46052996-46053015 None:intergenic 55.0%
GGTGGTCACTGGGATCTTTC+TGG + chr4_1:46052882-46052901 Msa0550530:CDS 55.0%
GCTGGCCATAATGCTTGACC+GGG - chr4_1:46052996-46053015 None:intergenic 55.0%
GGTGAGAATCCCTCCCAAGG+AGG - chr4_1:46052965-46052984 None:intergenic 60.0%
!! TTGCCAGGTACTGGTGCCTG+AGG + chr4_1:46053128-46053147 Msa0550530:intron 60.0%
!! TGCCAGGTACTGGTGCCTGA+GGG + chr4_1:46053129-46053148 Msa0550530:intron 60.0%
TTCCCTCAGGCACCAGTACC+TGG - chr4_1:46053134-46053153 None:intergenic 60.0%
GGGCGGAACCGAAAGCCAAT+TGG + chr4_1:46053872-46053891 Msa0550530:three_prime_UTR 60.0%
GGTGAGAATCCCTCCCAAGG+AGG - chr4_1:46052965-46052984 None:intergenic 60.0%
!! TTGCCAGGTACTGGTGCCTG+AGG + chr4_1:46053128-46053147 Msa0550530:intron 60.0%
!! TGCCAGGTACTGGTGCCTGA+GGG + chr4_1:46053129-46053148 Msa0550530:intron 60.0%
TTCCCTCAGGCACCAGTACC+TGG - chr4_1:46053134-46053153 None:intergenic 60.0%
GGGCGGAACCGAAAGCCAAT+TGG + chr4_1:46053872-46053891 Msa0550530:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 46052731 46054350 46052731 ID=Msa0550530;Name=Msa0550530
chr4_1 mRNA 46052731 46054350 46052731 ID=Msa0550530-mRNA-1;Parent=Msa0550530;Name=Msa0550530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=141|1|1|1|1|1|2|737|106
chr4_1 exon 46052731 46053048 46052731 ID=Msa0550530-mRNA-1:exon:12198;Parent=Msa0550530-mRNA-1
chr4_1 exon 46053470 46054350 46053470 ID=Msa0550530-mRNA-1:exon:12199;Parent=Msa0550530-mRNA-1
chr4_1 five_prime_UTR 46052731 46052871 46052731 ID=Msa0550530-mRNA-1:five_prime_utr;Parent=Msa0550530-mRNA-1
chr4_1 CDS 46052872 46053048 46052872 ID=Msa0550530-mRNA-1:cds;Parent=Msa0550530-mRNA-1
chr4_1 CDS 46053470 46053613 46053470 ID=Msa0550530-mRNA-1:cds;Parent=Msa0550530-mRNA-1
chr4_1 three_prime_UTR 46053614 46054350 46053614 ID=Msa0550530-mRNA-1:three_prime_utr;Parent=Msa0550530-mRNA-1
Gene Sequence

>Msa0550530

ATGATTAGGTGGTGGTCACTGGGATCTTTCTGGTATGAGAAACTGAGCCGGAACTTTGTTCCTGTCCAGTGCACAAAACTAATATTTGTTCCTCCTTGGGAGGGATTCTCACCTAATTCATCCCGGTCAAGCATTATGGCCAGCATTTTGGATACTGAAAAGCAGCTTAATTTGCAGAATAATGCAAAGGATTCAAATCATGTGGACAGTTTGAAGCAATTGCTTCATAATCTGGACCTCTCTTATCGATTAGAATGGGTTGCGGAACGGAAAGTACTTCTTACAAGGCATGGTAATGAACTGGGCACTTTTCAGTTGTAG
Protein sequence

>Msa0550530

MIRWWSLGSFWYEKLSRNFVPVQCTKLIFVPPWEGFSPNSSRSSIMASILDTEKQLNLQNNAKDSNHVDSLKQLLHNLDLSYRLEWVAERKVLLTRHGNELGTFQL*