AlfalfaGEDB | Msa0553120

M. sativa cultivar zhongmu-4 / Msa0553120

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0553120	XP_003607287.1	97.674	301	5	2	1	301	1	299	0.0	607

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0553120	sp\|I1LPE9\|NAC6_SOYBN	77.346	309	49	9	1	301	1	296	2.71e-164	461

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0553120	G7JPL7	97.674	301	5	2	1	301	1	299	0.0	607

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TFs/TRs:

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Gene ID	Type	Classification
Msa0553120	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0418960	Msa0553120	0.806654	7.407499e-50	-8.615850e-47
Msa0464710	Msa0553120	0.801206	1.004394e-48	-8.615850e-47
Msa0553120	Msa0607180	0.856176	3.839649e-62	-8.615850e-47
Msa0553120	Msa0647360	0.842533	2.440285e-58	-8.615850e-47
Msa0553120	Msa1029090	0.803520	3.352301e-49	-8.615850e-47
Msa0553120	Msa1156180	0.800396	1.469702e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0553120	MtrunA17_Chr4g0038751	97.674	301	5	2	1	301	1	299	0.0	607
Msa0553120	MtrunA17_Chr4g0076731	53.947	152	65	2	14	162	53	202	2.46e-56	187
Msa0553120	MtrunA17_Chr8g0361731	45.771	201	89	5	4	188	1	197	1.51e-53	179
Msa0553120	MtrunA17_Chr6g0453131	46.231	199	88	5	16	199	16	210	4.78e-53	178
Msa0553120	MtrunA17_Chr5g0417001	43.684	190	90	3	12	188	11	196	4.95e-50	170
Msa0553120	MtrunA17_Chr2g0326361	39.744	234	118	5	17	238	16	238	1.34e-47	162
Msa0553120	MtrunA17_Chr2g0318101	40.476	210	103	5	6	198	5	209	1.63e-47	162
Msa0553120	MtrunA17_Chr5g0442771	37.339	233	124	4	12	222	15	247	2.27e-47	161
Msa0553120	MtrunA17_Chr5g0398971	41.791	201	106	4	17	208	9	207	3.31e-47	161
Msa0553120	MtrunA17_Chr7g0252971	43.750	192	88	4	9	180	1	192	4.50e-47	161

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0553120	AT2G17040.1	60.279	287	95	7	14	298	5	274	6.39e-112	325
Msa0553120	AT2G02450.1	53.642	151	65	2	14	161	50	198	2.80e-57	189
Msa0553120	AT2G02450.2	53.642	151	65	2	14	161	50	198	3.18e-57	190
Msa0553120	AT5G39820.1	58.647	133	54	1	12	144	17	148	5.35e-53	176
Msa0553120	AT1G26870.1	45.116	215	88	7	12	210	13	213	1.65e-50	172
Msa0553120	AT1G62700.1	47.771	157	73	3	17	167	9	162	7.69e-48	165
Msa0553120	AT1G62700.2	47.771	157	73	3	17	167	9	162	7.69e-48	165
Msa0553120	AT3G10480.2	51.974	152	63	3	17	161	29	177	2.77e-47	164
Msa0553120	AT3G10480.1	52.288	153	62	4	17	161	29	178	5.41e-47	164
Msa0553120	AT4G27410.2	51.266	158	68	5	13	164	11	165	1.91e-46	158

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Find 105 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCGTTATGATGTCAT+TGG	0.391717	4_1:+49972524	Msa0553120:CDS
AAAGGAGATTGTGTCCGTAG+AGG	0.637647	4_1:-49974157	None:intergenic
AAAGGTTTCTGGAAAGCCAC+CGG	0.569247	4_1:+49972815	Msa0553120:CDS
AAGAAGGTGGGGAAGATGTA+GGG	0.391402	4_1:-49974134	None:intergenic
AAGAGGAACCAAGACTGATT+GGG	0.506930	4_1:+49972925	Msa0553120:CDS
AATAATTAAAACTGAAGATA+TGG	0.309992	4_1:+49974263	Msa0553120:CDS
AATATACCTGGCAAGTCACA+AGG	0.637321	4_1:-49972572	None:intergenic
AATCGGGTTGAGGAAGACGT+TGG	0.622308	4_1:+49972880	Msa0553120:CDS
AATGACTTGTTGGAACAATA+AGG	0.302791	4_1:-49974217	None:intergenic
ACAAGCTAAATTAATTAGGC+AGG	0.504198	4_1:+49974600	Msa0553120:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTGTATTTATAAGGAATGGT+TGG	-	chr4_1:49972197-49972216	None:intergenic	30.0%
ATTATATATGGCATCAATGG+AGG	+	chr4_1:49972397-49972416	Msa0553120:exon	30.0%
AAACTTCGTTATGATGTCAT+TGG	+	chr4_1:49972524-49972543	Msa0553120:CDS	30.0%
TGATTGATTAGCAATATACC+TGG	-	chr4_1:49972587-49972606	None:intergenic	30.0%
TATATAGGTTTGTCAAAGGT+TGG	+	chr4_1:49972716-49972735	Msa0553120:intron	30.0%
ATATAGGTTTGTCAAAGGTT+GGG	+	chr4_1:49972717-49972736	Msa0553120:intron	30.0%
ATATACAATACCTTAGGCAA+GGG	-	chr4_1:49972983-49973002	None:intergenic	30.0%
TATATACAATACCTTAGGCA+AGG	-	chr4_1:49972984-49973003	None:intergenic	30.0%
TCAATTAGTCATCGTATGAT+AGG	-	chr4_1:49973075-49973094	None:intergenic	30.0%
AAAAAGATTCCCCTGTTATT+TGG	-	chr4_1:49973657-49973676	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	49972189	49974838	49972189	ID=Msa0553120;Name=Msa0553120
chr4_1	mRNA	49972189	49974838	49972189	ID=Msa0553120-mRNA-1;Parent=Msa0553120;Name=Msa0553120-mRNA-1;_AED=0.14;_eAED=0.14;_QI=215\|1\|1\|1\|1\|1\|3\|350\|301
chr4_1	exon	49972189	49972587	49972189	ID=Msa0553120-mRNA-1:exon:14094;Parent=Msa0553120-mRNA-1
chr4_1	exon	49972723	49972991	49972723	ID=Msa0553120-mRNA-1:exon:14095;Parent=Msa0553120-mRNA-1
chr4_1	exon	49974036	49974838	49974036	ID=Msa0553120-mRNA-1:exon:14096;Parent=Msa0553120-mRNA-1
chr4_1	five_prime_UTR	49972189	49972403	49972189	ID=Msa0553120-mRNA-1:five_prime_utr;Parent=Msa0553120-mRNA-1
chr4_1	CDS	49972404	49972587	49972404	ID=Msa0553120-mRNA-1:cds;Parent=Msa0553120-mRNA-1
chr4_1	CDS	49972723	49972991	49972723	ID=Msa0553120-mRNA-1:cds;Parent=Msa0553120-mRNA-1
chr4_1	CDS	49974036	49974488	49974036	ID=Msa0553120-mRNA-1:cds;Parent=Msa0553120-mRNA-1
chr4_1	three_prime_UTR	49974489	49974838	49974489	ID=Msa0553120-mRNA-1:three_prime_utr;Parent=Msa0553120-mRNA-1

Gene Sequence

>Msa0553120

ATGGCATCAATGGAGGACATCAACATGTGTACTGAACAAATTACACTTCCAGGATTCAGATTTCACCCCACAGAAGAAGAACTCCTTGATTTCTATCTTAAGAATATGGTTGTTGGCAAAAAACTTCGTTATGATGTCATTGGTTTTCTAAATATCTATCACCATGATCCTTGTGACTTGCCAGGTTTGTCAAAGGTTGGGGAAAGAGAATGGTATTTTTTCGTGCCAAGGGACAAAAAACATGGTAGTGGAGGAAGGCCTAACCGCACAACTGAGAAAGGTTTCTGGAAAGCCACCGGTTCGGACCGGAAAATCGTGACCTTGTCGGATCCTAAACGCATAATCGGGTTGAGGAAGACGTTGGTTTTCTATGAGGGAAGAGCTCCAAGAGGAACCAAGACTGATTGGGTTATGAATGAATACCGTTTACCTGACAATAGCCCCTTGCCTAAGGACATAGTATTATGCAAGATATATAGAAAGGCAACTTCCTTGAAAGTGCTTGAACAAAGAGCAGCAGAAGAAGAAGAGATGAAACAAATGGTGGGTTCCCCTACATCTTCCCCACCTTCTTCCTCTACGGACACAATCTCCTTTAACTATACACAACAAGACCAACAACACATGTCCATGCCCTTATTGTTCCAACAAGTCATTCCTAAGAAAGAATCTGAACATGAAATAATTAAAACTGAAGATATGGTTTCTTTGTCAAGTCATGAAAAAATTGTGAAAGATAACAAAAAAGCTTGTGGAACATCTTTGCAATTGCCCTTAGGGAAGGACAATGTACCTGAGCTTCAGTTACCTATAGCTGCAGATTGGACCCAAGATACGTTTTGGGCTCAGCTTAATAGTCCTTGGCTACAAAACTGGAGTGCTTACACCAATATTTTGAATTTTTAA

Protein sequence

>Msa0553120

MASMEDINMCTEQITLPGFRFHPTEEELLDFYLKNMVVGKKLRYDVIGFLNIYHHDPCDLPGLSKVGEREWYFFVPRDKKHGSGGRPNRTTEKGFWKATGSDRKIVTLSDPKRIIGLRKTLVFYEGRAPRGTKTDWVMNEYRLPDNSPLPKDIVLCKIYRKATSLKVLEQRAAEEEEMKQMVGSPTSSPPSSSTDTISFNYTQQDQQHMSMPLLFQQVIPKKESEHEIIKTEDMVSLSSHEKIVKDNKKACGTSLQLPLGKDNVPELQLPIAADWTQDTFWAQLNSPWLQNWSAYTNILNF*