AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0553180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553180 MtrunA17_Chr4g0038851 96.398 472 17 0 4 475 1 472 0.0 945
Msa0553180 MtrunA17_Chr5g0396491 74.948 479 112 6 4 475 4 481 0.0 736
Msa0553180 MtrunA17_Chr3g0085681 49.888 445 213 4 30 474 4 438 3.40e-162 466
Msa0553180 MtrunA17_Chr3g0085361 51.268 355 162 5 120 474 9 352 3.08e-128 376
Msa0553180 MtrunA17_Chr7g0224271 34.000 450 215 6 40 472 289 673 4.70e-76 251
Msa0553180 MtrunA17_Chr5g0447691 31.323 431 230 6 48 472 174 544 9.11e-67 223
Msa0553180 MtrunA17_Chr1g0159461 29.604 429 236 8 50 472 224 592 3.76e-56 196
Msa0553180 MtrunA17_Chr3g0125811 29.138 429 238 8 50 472 202 570 7.45e-55 192
Msa0553180 MtrunA17_Chr2g0319091 30.698 430 232 8 48 471 209 578 4.09e-54 190
Msa0553180 MtrunA17_Chr3g0101141 27.703 444 254 8 35 472 160 542 2.75e-52 185
Msa0553180 MtrunA17_Chr4g0057891 28.604 444 235 11 45 472 143 520 5.99e-50 178
Msa0553180 MtrunA17_Chr4g0031721 27.972 429 240 7 51 471 316 683 2.41e-49 179
Msa0553180 MtrunA17_Chr7g0238891 27.103 428 247 5 51 472 372 740 1.43e-47 175
Msa0553180 MtrunA17_Chr2g0306641 28.733 442 236 13 42 473 116 488 1.58e-47 171
Msa0553180 MtrunA17_Chr4g0031701 26.932 453 262 8 24 467 229 621 7.19e-47 171
Msa0553180 MtrunA17_Chr2g0290241 27.804 428 242 9 52 472 58 425 7.31e-47 167
Msa0553180 MtrunA17_Chr4g0031731 26.711 453 263 8 24 467 229 621 5.51e-45 166
Msa0553180 MtrunA17_Chr2g0328391 26.168 428 251 5 51 471 299 668 5.54e-44 164
Msa0553180 MtrunA17_Chr4g0031711 27.174 460 261 11 23 471 336 732 1.18e-43 164
Msa0553180 MtrunA17_Chr2g0328411 26.147 436 236 8 51 471 289 653 1.99e-41 157
Msa0553180 MtrunA17_Chr2g0328431 26.230 427 247 7 51 471 289 653 4.90e-41 155
Msa0553180 MtrunA17_Chr2g0328341 25.721 451 266 9 26 471 251 637 6.89e-41 155
Msa0553180 MtrunA17_Chr2g0328381 25.629 437 260 9 40 471 264 640 2.32e-40 153
Msa0553180 MtrunA17_Chr4g0057291 35.814 215 134 1 44 254 285 499 5.35e-39 150
Msa0553180 MtrunA17_Chr4g0057291 32.026 153 97 5 325 471 510 661 5.90e-19 90.1
Msa0553180 MtrunA17_Chr2g0294471 29.083 447 236 15 42 472 195 576 4.20e-37 143
Msa0553180 MtrunA17_Chr1g0183641 28.146 437 237 12 43 474 115 479 4.22e-37 142
Msa0553180 MtrunA17_Chr7g0245601 39.791 191 112 2 55 244 442 630 8.23e-37 144
Msa0553180 MtrunA17_Chr7g0245601 37.584 149 88 3 327 472 646 792 5.96e-21 96.3
Msa0553180 MtrunA17_Chr2g0328401 26.720 378 209 6 51 422 290 605 1.27e-36 142
Msa0553180 MtrunA17_Chr8g0357601 43.333 150 83 1 323 472 405 552 1.29e-36 142
Msa0553180 MtrunA17_Chr8g0357601 31.855 248 144 7 44 269 175 419 5.06e-25 108
Msa0553180 MtrunA17_Chr1g0200401 28.228 457 230 16 45 473 74 460 2.91e-36 139
Msa0553180 MtrunA17_Chr2g0294461 27.051 451 243 12 42 472 201 585 1.40e-35 139
Msa0553180 MtrunA17_Chr3g0110971 33.784 222 133 4 39 255 207 419 1.90e-33 133
Msa0553180 MtrunA17_Chr3g0110971 36.196 163 91 2 323 472 425 587 8.84e-28 116
Msa0553180 MtrunA17_Chr8g0376381 32.589 224 132 5 39 255 157 368 1.47e-30 124
Msa0553180 MtrunA17_Chr8g0376381 35.762 151 94 2 323 472 374 522 9.08e-28 116
Msa0553180 MtrunA17_Chr3g0114841 25.490 459 251 14 38 471 107 499 1.67e-30 124
Msa0553180 MtrunA17_Chr5g0445331 31.111 225 146 4 35 251 146 369 3.08e-29 120
Msa0553180 MtrunA17_Chr5g0445331 38.298 141 86 1 332 472 393 532 5.03e-26 110
Msa0553180 MtrunA17_Chr7g0270031 25.567 485 278 13 4 472 137 554 6.33e-29 119
Msa0553180 MtrunA17_Chr4g0031741 30.631 222 141 4 51 266 284 498 1.15e-28 119
Msa0553180 MtrunA17_Chr4g0031741 32.000 150 101 1 323 471 500 649 7.49e-17 83.6
Msa0553180 MtrunA17_Chr2g0328361 29.694 229 155 3 40 266 263 487 1.50e-28 119
Msa0553180 MtrunA17_Chr2g0328371 25.000 292 158 7 134 422 13 246 8.05e-27 108
Msa0553180 MtrunA17_Chr1g0207361 63.725 102 35 2 276 376 64 164 1.22e-26 105
Msa0553180 MtrunA17_Chr1g0207361 72.222 54 15 0 85 138 1 54 7.74e-22 92.4
Msa0553180 MtrunA17_Chr4g0038761 31.606 193 129 2 55 244 88 280 9.40e-26 109
Msa0553180 MtrunA17_Chr4g0038761 40.268 149 83 4 326 472 298 442 9.82e-21 94.7
Msa0553180 MtrunA17_Chr4g0053901 23.984 492 294 11 14 471 22 467 6.33e-25 107
Msa0553180 MtrunA17_Chr5g0401421 24.124 485 285 14 22 471 9 445 1.41e-24 105
Msa0553180 MtrunA17_Chr4g0076091 31.163 215 140 3 46 253 170 383 2.09e-24 106
Msa0553180 MtrunA17_Chr4g0076091 34.228 149 97 1 323 471 395 542 1.28e-21 97.8
Msa0553180 MtrunA17_Chr5g0424131 30.303 231 144 4 37 250 107 337 5.37e-24 104
Msa0553180 MtrunA17_Chr5g0401131 31.193 218 140 4 45 253 108 324 8.22e-24 103
Msa0553180 MtrunA17_Chr1g0194621 34.911 169 100 5 310 472 354 518 7.41e-22 98.6
Msa0553180 MtrunA17_Chr1g0194621 25.200 250 166 8 40 269 135 383 1.16e-12 70.1
Msa0553180 MtrunA17_Chr2g0329591 30.556 216 138 5 42 247 65 278 2.72e-21 96.3
Msa0553180 MtrunA17_Chr6g0468561 27.171 357 188 11 127 472 367 662 1.67e-19 91.7
Msa0553180 MtrunA17_Chr4g0039661 28.251 223 150 3 48 263 190 409 1.91e-19 91.3
Msa0553180 MtrunA17_Chr4g0039661 30.601 183 114 6 298 472 378 555 3.01e-14 75.1
Msa0553180 MtrunA17_Chr7g0236851 31.004 229 131 9 257 471 441 656 7.17e-19 89.7
Msa0553180 MtrunA17_Chr7g0230731 33.529 170 105 3 22 191 349 510 3.95e-15 78.2
Msa0553180 MtrunA17_Chr7g0230771 33.529 170 105 3 22 191 352 513 4.81e-15 77.8
Msa0553180 MtrunA17_Chr8g0383981 28.641 206 133 8 48 248 136 332 6.44e-13 70.9
Msa0553180 MtrunA17_Chr3g0087711 51.724 58 28 0 202 259 1 58 8.67e-13 63.2
Msa0553180 MtrunA17_Chr2g0323111 26.174 149 108 2 323 471 159 305 4.16e-12 67.0
Msa0553180 MtrunA17_Chr8g0344101 22.629 464 249 14 45 475 217 603 6.41e-12 67.8
Msa0553180 MtrunA17_Chr4g0012071 31.351 185 113 4 52 230 197 373 1.53e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553180 AT1G50420.1 62.190 484 172 5 1 475 1 482 0.0 614
Msa0553180 AT1G66350.1 32.265 437 220 9 39 472 143 506 6.66e-67 223
Msa0553180 AT5G48150.1 30.445 427 232 5 52 472 123 490 1.05e-65 219
Msa0553180 AT5G48150.4 30.445 427 232 5 52 472 123 490 1.05e-65 219
Msa0553180 AT5G48150.2 30.445 427 232 5 52 472 123 490 1.05e-65 219
Msa0553180 AT5G48150.3 30.445 427 232 5 52 472 123 490 1.05e-65 219
Msa0553180 AT2G04890.1 32.464 422 224 5 51 472 53 413 1.54e-64 214
Msa0553180 AT5G17490.1 30.022 453 233 11 34 472 134 516 3.93e-58 200
Msa0553180 AT1G21450.1 30.769 429 232 7 50 472 224 593 1.83e-56 197
Msa0553180 AT1G50600.3 29.322 457 251 9 27 472 131 526 6.43e-56 194
Msa0553180 AT1G50600.2 29.322 457 251 9 27 472 206 601 2.69e-55 194
Msa0553180 AT1G63100.1 29.438 445 238 8 43 471 244 628 7.05e-55 194
Msa0553180 AT1G63100.2 29.438 445 238 8 43 471 269 653 8.54e-55 194
Msa0553180 AT1G50600.1 29.322 457 251 10 27 472 202 597 1.59e-54 192
Msa0553180 AT1G14920.1 29.438 445 228 12 39 472 160 529 3.31e-51 182
Msa0553180 AT5G41920.1 29.561 433 229 11 48 474 40 402 3.59e-45 163
Msa0553180 AT1G07530.1 27.727 440 228 10 51 471 396 764 6.63e-44 165
Msa0553180 AT2G29060.2 27.146 431 244 10 51 471 284 654 2.03e-40 154
Msa0553180 AT3G54220.1 41.706 211 121 1 36 244 278 488 2.06e-40 154
Msa0553180 AT3G54220.1 36.301 146 91 2 329 474 509 652 1.31e-20 95.5
Msa0553180 AT2G29060.1 27.189 434 246 10 48 471 318 691 2.20e-40 154
Msa0553180 AT1G07520.2 25.508 443 262 7 40 471 143 528 2.80e-40 152
Msa0553180 AT1G07520.4 25.508 443 262 7 40 471 143 528 2.80e-40 152
Msa0553180 AT1G07520.3 25.508 443 262 7 40 471 308 693 1.65e-39 152
Msa0553180 AT1G07520.1 25.508 443 262 7 40 471 307 692 1.69e-39 152
Msa0553180 AT5G66770.1 28.899 436 231 12 48 472 217 584 3.90e-39 149
Msa0553180 AT5G59450.1 26.037 434 253 7 48 472 224 598 4.20e-38 147
Msa0553180 AT2G29065.1 25.799 438 246 10 51 471 252 627 1.19e-37 146
Msa0553180 AT2G37650.1 24.594 431 258 6 48 472 344 713 1.42e-36 143
Msa0553180 AT2G37650.2 24.594 431 258 6 48 472 332 701 1.53e-36 143
Msa0553180 AT3G46600.2 25.926 432 254 6 50 475 3 374 3.58e-36 137
Msa0553180 AT3G46600.3 25.926 432 254 6 50 475 179 550 2.76e-35 138
Msa0553180 AT3G46600.1 25.926 432 254 6 50 475 211 582 6.05e-35 137
Msa0553180 AT2G01570.1 33.480 227 131 5 39 255 212 428 6.55e-31 126
Msa0553180 AT2G01570.1 37.086 151 91 3 323 472 434 581 1.31e-26 113
Msa0553180 AT5G52510.1 26.147 436 247 13 50 472 267 640 1.35e-30 125
Msa0553180 AT3G03450.1 30.942 223 145 5 39 255 171 390 2.35e-29 121
Msa0553180 AT3G03450.1 38.854 157 94 2 317 472 390 545 1.53e-28 119
Msa0553180 AT1G55580.1 37.584 149 92 1 325 472 297 445 9.47e-28 115
Msa0553180 AT1G55580.1 27.451 255 144 8 47 261 40 293 2.32e-12 69.3
Msa0553180 AT4G37650.1 31.579 247 139 7 24 253 131 364 1.49e-23 103
Msa0553180 AT4G37650.1 25.871 201 126 7 289 472 335 529 1.47e-11 67.0
Msa0553180 AT4G17230.1 34.000 150 98 1 323 472 377 525 7.64e-23 102
Msa0553180 AT4G17230.1 28.972 214 145 2 46 253 153 365 1.39e-22 101
Msa0553180 AT4G17230.2 34.000 150 98 1 323 472 377 525 7.64e-23 102
Msa0553180 AT4G17230.2 28.972 214 145 2 46 253 153 365 1.39e-22 101
Msa0553180 AT4G08250.1 29.730 259 154 9 6 250 72 316 1.80e-20 94.4
Msa0553180 AT3G50650.1 31.455 213 127 5 56 260 180 381 3.16e-17 84.7
Msa0553180 AT3G50650.1 29.530 149 98 3 331 472 394 542 7.81e-14 74.3
Msa0553180 AT3G49950.1 21.692 461 278 13 29 471 12 407 7.69e-15 76.6
Msa0553180 AT4G00150.1 27.041 196 132 6 283 474 368 556 5.03e-12 68.6

Find 129 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACCTCATTGGAGAGATTT+AGG 0.163267 4_1:+50106209 Msa0553180:CDS
TTCTTCCTTAGTCATGATTT+TGG 0.181091 4_1:+50104761 Msa0553180:exon
AATGAGCCTCTAAGTTATTT+TGG 0.189632 4_1:+50106355 Msa0553180:CDS
TTTCATCATCCCAGGCTAAA+AGG 0.243724 4_1:-50105841 None:intergenic
CCGAATGTGCTTCGGTTTAC+TGG 0.250507 4_1:-50105929 None:intergenic
TGCAGCACTATTTGATTGTT+TGG 0.256288 4_1:+50106171 Msa0553180:CDS
CAATCCTGTTGTCAGCAAAT+TGG 0.264169 4_1:+50105742 Msa0553180:CDS
CATCATGGAGAAGAGCTTTA+AGG 0.269652 4_1:-50105132 None:intergenic
CAGAACAAAGTAACTCATTA+AGG 0.282442 4_1:+50104632 Msa0553180:five_prime_UTR
AGATTTGATTTAGCTGGATT+TGG 0.318965 4_1:+50106331 Msa0553180:CDS
CAATATCAGCTTGGAGATCT+AGG 0.325494 4_1:+50106485 Msa0553180:CDS
AGAGTGTGAGTTTGAACTTT+TGG 0.331382 4_1:-50104586 None:intergenic
ATGCCACCTTCATCTGGCTT+TGG 0.334564 4_1:+50105152 Msa0553180:CDS
AGCATTTGTTCTCACAAATC+AGG 0.349043 4_1:+50105499 Msa0553180:CDS
TCCATGATGCCACCTTCATC+TGG 0.349847 4_1:+50105146 Msa0553180:CDS
AACTTACCAAAATAACTTAG+AGG 0.353618 4_1:-50106361 None:intergenic
AATCTCTCCAATGAGGTTCT+TGG 0.353686 4_1:-50106205 None:intergenic
AACCTCATTGGAGAGATTTA+GGG 0.354349 4_1:+50106210 Msa0553180:CDS
TGCTGACAACAGGATTGAAT+TGG 0.362322 4_1:-50105736 None:intergenic
TTTGGACGAGCACTTGAGTT+TGG 0.364682 4_1:+50106527 Msa0553180:three_prime_UTR
CAATTTGCTTGAGAAAGATT+TGG 0.366215 4_1:+50105952 Msa0553180:CDS
ATCTCCACACCCTTGGCTTA+AGG 0.366272 4_1:+50105175 Msa0553180:CDS
GAGTTACTTTGTTCTGTTGT+TGG 0.378375 4_1:-50104625 None:intergenic
TATAGAATGAAGGAGGAAAA+TGG 0.386592 4_1:+50106424 Msa0553180:CDS
TGAACTTGAGTCAGGACTTA+TGG 0.391992 4_1:-50105987 None:intergenic
ACAAGTATGCAATTCCTTGA+TGG 0.395999 4_1:-50104738 None:intergenic
CAAATCCAAGAGGCAAGCTT+TGG 0.398712 4_1:+50104671 Msa0553180:five_prime_UTR
CTCAAATGTGGAGGGCCATC+AGG 0.400333 4_1:-50105626 None:intergenic
GAGAAACTTCGTGTTAAAAC+CGG 0.400451 4_1:+50105779 Msa0553180:CDS
CAGGATGAGCACTCAAAACT+TGG 0.403034 4_1:-50105607 None:intergenic
TTACCGAAGCACTTGCTGAT+AGG 0.405714 4_1:+50105360 Msa0553180:CDS
ATCTCTCCAATGAGGTTCTT+GGG 0.409565 4_1:-50106204 None:intergenic
CACGAAGTTTCTCAAAATCA+AGG 0.414533 4_1:-50105769 None:intergenic
GCTTTGGATCTCCACACCCT+TGG 0.415132 4_1:+50105168 Msa0553180:CDS
GAGTAGAGTTGAGCGCTTTG+TGG 0.416341 4_1:-50105406 None:intergenic
TTTGAAGTGTTCAAAGAAAC+CGG 0.422703 4_1:-50106016 None:intergenic
AGTAAGACGATGTGATTGTT+TGG 0.435895 4_1:+50106509 Msa0553180:exon
TTTATGAGATTTGATTTAGC+TGG 0.436460 4_1:+50106325 Msa0553180:CDS
AGGCTAAAAGGGTATGCAAT+TGG 0.439239 4_1:-50105829 None:intergenic
TTGAGCGCTTTGTGGATTCC+AGG 0.446871 4_1:-50105398 None:intergenic
CAAGTATGCAATTCCTTGAT+GGG 0.447226 4_1:-50104737 None:intergenic
GGAACTGAAATCTGAGGAAA+GGG 0.447352 4_1:+50105196 Msa0553180:CDS
AAGCGGTTCATGCAGAGTTA+TGG 0.447737 4_1:+50106391 Msa0553180:CDS
CCAGTAAACCGAAGCACATT+CGG 0.448222 4_1:+50105929 Msa0553180:CDS
CTTCATGGTTTCATCATCCC+AGG 0.449792 4_1:-50105849 None:intergenic
GAACAAGACTCTAATCATAA+TGG 0.450476 4_1:+50106106 Msa0553180:CDS
TGCTAATATGCTGGCAAGAT+AGG 0.451606 4_1:+50106452 Msa0553180:CDS
TCTGTTACAACCATCACCTT+TGG 0.451615 4_1:-50106085 None:intergenic
AAATTCCAAAGCTTGCCTCT+TGG 0.452365 4_1:-50104676 None:intergenic
AACGATCCCAAGAACCTCAT+TGG 0.456113 4_1:+50106198 Msa0553180:CDS
AAAGGAAAGGCATGAAAAGC+TGG 0.456379 4_1:+50106294 Msa0553180:CDS
CGCTGCATAGTATCACCGTC+AGG 0.465542 4_1:-50105323 None:intergenic
GCAAATTGCCGAATGTGCTT+CGG 0.471544 4_1:-50105937 None:intergenic
TTCATCATCCCAGGCTAAAA+GGG 0.472082 4_1:-50105840 None:intergenic
TCCATGCTTGCTTCCCCTGA+CGG 0.473491 4_1:+50105308 Msa0553180:CDS
CAACTCTACTCGAGTGTCAA+TGG 0.477544 4_1:+50105418 Msa0553180:CDS
TGAAGAGTAATGAAGAAGAA+AGG 0.478667 4_1:-50104705 None:intergenic
CAAGCATGGAAATTTGATCA+AGG 0.482089 4_1:-50105295 None:intergenic
TTGCACAAGTGAGCAACAAA+TGG 0.493036 4_1:-50105232 None:intergenic
ATGTGCTTCGGTTTACTGGA+TGG 0.504499 4_1:-50105925 None:intergenic
ACAAATGCTACTTTCATGAA+AGG 0.509715 4_1:-50105485 None:intergenic
AGGATACTTAAAGCATGGCC+TGG 0.511478 4_1:+50105380 Msa0553180:CDS
TTCAACTTCAAAACCCATCA+AGG 0.512600 4_1:+50104724 Msa0553180:five_prime_UTR
TTGTAAGTGAATTCCATTCG+AGG 0.515092 4_1:-50105897 None:intergenic
CAAATGCTACTTTCATGAAA+GGG 0.516070 4_1:-50105484 None:intergenic
TTACTTTGTTCTGTTGTTGG+TGG 0.520167 4_1:-50104622 None:intergenic
TCAGGCTATCATTGAAGCAA+TGG 0.520201 4_1:+50105517 Msa0553180:CDS
GCCAGATGAAGGTGGCATCA+TGG 0.521665 4_1:-50105147 None:intergenic
ACATACCAAAATCATGACTA+AGG 0.527098 4_1:-50104766 None:intergenic
AAGAATATTATTGCATGTGA+GGG 0.527409 4_1:+50106262 Msa0553180:CDS
CAAGAATATTATTGCATGTG+AGG 0.529680 4_1:+50106261 Msa0553180:CDS
TCGAATGGAATTCACTTACA+AGG 0.530422 4_1:+50105899 Msa0553180:CDS
AGAAACTTCGTGTTAAAACC+GGG 0.531168 4_1:+50105780 Msa0553180:CDS
GGCATGAAAAGCTGGATAAG+TGG 0.531394 4_1:+50106302 Msa0553180:CDS
GGTGATGATGAACTTGAGTC+AGG 0.534611 4_1:-50105995 None:intergenic
ATGTGAGGGATTAGAAAGAA+AGG 0.534942 4_1:+50106276 Msa0553180:CDS
TATCCTATCAGCAAGTGCTT+CGG 0.537933 4_1:-50105363 None:intergenic
ACCGTCAGGGGAAGCAAGCA+TGG 0.541937 4_1:-50105309 None:intergenic
ACTAATTGCTAGTGCCTCCC+CGG 0.543403 4_1:-50105798 None:intergenic
TGACTCAAGTTCATCATCAC+CGG 0.544363 4_1:+50105997 Msa0553180:CDS
AGGAACTGAAATCTGAGGAA+AGG 0.544931 4_1:+50105195 Msa0553180:CDS
GATTTCAGTTCCTTAAGCCA+AGG 0.546043 4_1:-50105185 None:intergenic
TTGTGAGGCATATAGAATGA+AGG 0.546355 4_1:+50106414 Msa0553180:CDS
GTACAGCTATGTTGCAAGAA+GGG 0.546911 4_1:+50105087 Msa0553180:intron
AGAAATGAAATCCACTGAGC+AGG 0.550035 4_1:-50105584 None:intergenic
CCAAGCTGATATTGAAAACA+AGG 0.551538 4_1:-50106476 None:intergenic
AGTTCCTTAAGCCAAGGGTG+TGG 0.551766 4_1:-50105179 None:intergenic
ATGGCTGTGTGCTAATATGC+TGG 0.553571 4_1:+50106443 Msa0553180:CDS
CAATCAGTCTATGAGCTACC+TGG 0.553683 4_1:-50105685 None:intergenic
CCTCCACATTTGAGAATCAC+CGG 0.556070 4_1:+50105635 Msa0553180:CDS
GAAGAAAGAGGTTCTCGACC+AGG 0.556759 4_1:+50105667 Msa0553180:CDS
ATTTCAGTTCCTTAAGCCAA+GGG 0.557071 4_1:-50105184 None:intergenic
CTCTTTCTTCTGATGAACGC+CGG 0.562200 4_1:-50105654 None:intergenic
AGTGTAGAGCTTCTAGTAGC+CGG 0.564547 4_1:-50106144 None:intergenic
AAGCATGGAAATTTGATCAA+GGG 0.565367 4_1:-50105294 None:intergenic
GGTACAGCTATGTTGCAAGA+AGG 0.572547 4_1:+50105086 Msa0553180:intron
GGAGATCCAAAGCCAGATGA+AGG 0.572774 4_1:-50105158 None:intergenic
CTCATTGGAGAGATTTAGGG+TGG 0.573947 4_1:+50106213 Msa0553180:CDS
GAGTTTATCACCAAAGGTGA+TGG 0.577759 4_1:+50106075 Msa0553180:CDS
ACAACAGGATTGAATTGGAA+TGG 0.580239 4_1:-50105731 None:intergenic
CATGCAGAGTTATGGTTGTG+AGG 0.584714 4_1:+50106399 Msa0553180:CDS
TACAAAATACTTTCATACAA+AGG 0.591122 4_1:-50106669 None:intergenic
GCTATCATTGAAGCAATGGA+AGG 0.591127 4_1:+50105521 Msa0553180:CDS
CGGCGTTCATCAGAAGAAAG+AGG 0.593327 4_1:+50105655 Msa0553180:CDS
ACTTGAGTTTGGCAAAATTG+CGG 0.593940 4_1:+50106538 Msa0553180:three_prime_UTR
TATCATTGAAGCAATGGAAG+GGG 0.594602 4_1:+50105523 Msa0553180:CDS
CTATCATTGAAGCAATGGAA+GGG 0.603065 4_1:+50105522 Msa0553180:CDS
CTGATAGGATACTTAAAGCA+TGG 0.603739 4_1:+50105375 Msa0553180:CDS
AGACATTTCTCAATGCATTG+TGG 0.605127 4_1:+50106053 Msa0553180:CDS
CATCACTCTTCAAATCCAAG+AGG 0.605474 4_1:+50104661 Msa0553180:five_prime_UTR
GCTTAAGGAACTGAAATCTG+AGG 0.605648 4_1:+50105190 Msa0553180:CDS
ATTGTGGAGTTTATCACCAA+AGG 0.618172 4_1:+50106069 Msa0553180:CDS
CGGTGATTCTCAAATGTGGA+GGG 0.619559 4_1:-50105634 None:intergenic
TGAGGCATATAGAATGAAGG+AGG 0.622680 4_1:+50106417 Msa0553180:CDS
AGGGATTAGAAAGAAAGGAA+AGG 0.622681 4_1:+50106281 Msa0553180:CDS
ACGCCGGTGATTCTCAAATG+TGG 0.623446 4_1:-50105638 None:intergenic
GCTGCATAGTATCACCGTCA+GGG 0.623909 4_1:-50105322 None:intergenic
CCGGTGATTCTCAAATGTGG+AGG 0.626889 4_1:-50105635 None:intergenic
CACCCTAAATCTCTCCAATG+AGG 0.633605 4_1:-50106212 None:intergenic
TGAGTTTGGCAAAATTGCGG+TGG 0.634786 4_1:+50106541 Msa0553180:three_prime_UTR
CTGCATAGTATCACCGTCAG+GGG 0.640877 4_1:-50105321 None:intergenic
ATGCTGCTGAACCTGCTCAG+TGG 0.654432 4_1:+50105573 Msa0553180:CDS
GATCCAAAGCCAGATGAAGG+TGG 0.658186 4_1:-50105155 None:intergenic
CAGCGAGATTGACAAAATCA+CGG 0.671324 4_1:-50106568 None:intergenic
GAAACTTCGTGTTAAAACCG+GGG 0.688408 4_1:+50105781 Msa0553180:CDS
TTGGTAAGTTGCAAGCAAAG+CGG 0.705559 4_1:+50106374 Msa0553180:CDS
GAACTGAAATCTGAGGAAAG+GGG 0.705778 4_1:+50105197 Msa0553180:CDS
CTGACGGTGATACTATGCAG+CGG 0.719141 4_1:+50105324 Msa0553180:CDS
ACTTCGTGTTAAAACCGGGG+AGG 0.735326 4_1:+50105784 Msa0553180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTATTGTCATTA+TGG - chr4_1:50106622-50106641 None:intergenic 15.0%
!! GACAATAAGAAAAAAAATAA+AGG + chr4_1:50106626-50106645 Msa0553180:three_prime_UTR 15.0%
!!! TTTTTTTCTTATTGTCATTA+TGG - chr4_1:50106622-50106641 None:intergenic 15.0%
!! GACAATAAGAAAAAAAATAA+AGG + chr4_1:50106626-50106645 Msa0553180:three_prime_UTR 15.0%
!!! TCATTTCATTTTATGTTACA+TGG + chr4_1:50105065-50105084 Msa0553180:intron 20.0%
!! TACAAAATACTTTCATACAA+AGG - chr4_1:50106672-50106691 None:intergenic 20.0%
!!! TCATTTCATTTTATGTTACA+TGG + chr4_1:50105065-50105084 Msa0553180:intron 20.0%
!! TACAAAATACTTTCATACAA+AGG - chr4_1:50106672-50106691 None:intergenic 20.0%
!! AAAAGTTATGGACTAAAACA+TGG - chr4_1:50104824-50104843 None:intergenic 25.0%
! AAGAATATTATTGCATGTGA+GGG + chr4_1:50106262-50106281 Msa0553180:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG + chr4_1:50106325-50106344 Msa0553180:CDS 25.0%
! AACTTACCAAAATAACTTAG+AGG - chr4_1:50106364-50106383 None:intergenic 25.0%
!! AAAAGTTATGGACTAAAACA+TGG - chr4_1:50104824-50104843 None:intergenic 25.0%
! AAGAATATTATTGCATGTGA+GGG + chr4_1:50106262-50106281 Msa0553180:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG + chr4_1:50106325-50106344 Msa0553180:CDS 25.0%
! AACTTACCAAAATAACTTAG+AGG - chr4_1:50106364-50106383 None:intergenic 25.0%
CAGAACAAAGTAACTCATTA+AGG + chr4_1:50104632-50104651 Msa0553180:five_prime_UTR 30.0%
TGAAGAGTAATGAAGAAGAA+AGG - chr4_1:50104708-50104727 None:intergenic 30.0%
! TTCTTCCTTAGTCATGATTT+TGG + chr4_1:50104761-50104780 Msa0553180:exon 30.0%
ACATACCAAAATCATGACTA+AGG - chr4_1:50104769-50104788 None:intergenic 30.0%
! TCCATAACTTTTTGATGAGT+CGG + chr4_1:50104832-50104851 Msa0553180:intron 30.0%
ATGAGTCTACTTGAGAATAT+TGG + chr4_1:50104974-50104993 Msa0553180:intron 30.0%
AAGCATGGAAATTTGATCAA+GGG - chr4_1:50105297-50105316 None:intergenic 30.0%
!! CAAATGCTACTTTCATGAAA+GGG - chr4_1:50105487-50105506 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG - chr4_1:50105488-50105507 None:intergenic 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG - chr4_1:50105867-50105886 None:intergenic 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG - chr4_1:50105884-50105903 None:intergenic 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG + chr4_1:50105884-50105903 Msa0553180:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG + chr4_1:50105952-50105971 Msa0553180:CDS 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG - chr4_1:50106019-50106038 None:intergenic 30.0%
GAACAAGACTCTAATCATAA+TGG + chr4_1:50106106-50106125 Msa0553180:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG + chr4_1:50106120-50106139 Msa0553180:CDS 30.0%
CAAGAATATTATTGCATGTG+AGG + chr4_1:50106261-50106280 Msa0553180:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG + chr4_1:50106331-50106350 Msa0553180:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG + chr4_1:50106355-50106374 Msa0553180:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG + chr4_1:50106424-50106443 Msa0553180:CDS 30.0%
CAGAACAAAGTAACTCATTA+AGG + chr4_1:50104632-50104651 Msa0553180:five_prime_UTR 30.0%
TGAAGAGTAATGAAGAAGAA+AGG - chr4_1:50104708-50104727 None:intergenic 30.0%
! TTCTTCCTTAGTCATGATTT+TGG + chr4_1:50104761-50104780 Msa0553180:exon 30.0%
ACATACCAAAATCATGACTA+AGG - chr4_1:50104769-50104788 None:intergenic 30.0%
! TCCATAACTTTTTGATGAGT+CGG + chr4_1:50104832-50104851 Msa0553180:intron 30.0%
ATGAGTCTACTTGAGAATAT+TGG + chr4_1:50104974-50104993 Msa0553180:intron 30.0%
AAGCATGGAAATTTGATCAA+GGG - chr4_1:50105297-50105316 None:intergenic 30.0%
!! CAAATGCTACTTTCATGAAA+GGG - chr4_1:50105487-50105506 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG - chr4_1:50105488-50105507 None:intergenic 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG - chr4_1:50105867-50105886 None:intergenic 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG - chr4_1:50105884-50105903 None:intergenic 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG + chr4_1:50105884-50105903 Msa0553180:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG + chr4_1:50105952-50105971 Msa0553180:CDS 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG - chr4_1:50106019-50106038 None:intergenic 30.0%
GAACAAGACTCTAATCATAA+TGG + chr4_1:50106106-50106125 Msa0553180:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG + chr4_1:50106120-50106139 Msa0553180:CDS 30.0%
CAAGAATATTATTGCATGTG+AGG + chr4_1:50106261-50106280 Msa0553180:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG + chr4_1:50106331-50106350 Msa0553180:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG + chr4_1:50106355-50106374 Msa0553180:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG + chr4_1:50106424-50106443 Msa0553180:CDS 30.0%
! AGAGTGTGAGTTTGAACTTT+TGG - chr4_1:50104589-50104608 None:intergenic 35.0%
TTACTTTGTTCTGTTGTTGG+TGG - chr4_1:50104625-50104644 None:intergenic 35.0%
! GAGTTACTTTGTTCTGTTGT+TGG - chr4_1:50104628-50104647 None:intergenic 35.0%
TTCAACTTCAAAACCCATCA+AGG + chr4_1:50104724-50104743 Msa0553180:five_prime_UTR 35.0%
CAAGTATGCAATTCCTTGAT+GGG - chr4_1:50104740-50104759 None:intergenic 35.0%
ACAAGTATGCAATTCCTTGA+TGG - chr4_1:50104741-50104760 None:intergenic 35.0%
AGCTGTTACAAGAAGAAAAG+AGG - chr4_1:50104793-50104812 None:intergenic 35.0%
! ACCGACTCATCAAAAAGTTA+TGG - chr4_1:50104836-50104855 None:intergenic 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG - chr4_1:50105187-50105206 None:intergenic 35.0%
CAAGCATGGAAATTTGATCA+AGG - chr4_1:50105298-50105317 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG + chr4_1:50105375-50105394 Msa0553180:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG + chr4_1:50105499-50105518 Msa0553180:CDS 35.0%
CTATCATTGAAGCAATGGAA+GGG + chr4_1:50105522-50105541 Msa0553180:CDS 35.0%
TATCATTGAAGCAATGGAAG+GGG + chr4_1:50105523-50105542 Msa0553180:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG - chr4_1:50105734-50105753 None:intergenic 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG - chr4_1:50105749-50105768 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG - chr4_1:50105772-50105791 None:intergenic 35.0%
GAGAAACTTCGTGTTAAAAC+CGG + chr4_1:50105779-50105798 Msa0553180:CDS 35.0%
AGAAACTTCGTGTTAAAACC+GGG + chr4_1:50105780-50105799 Msa0553180:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG - chr4_1:50105900-50105919 None:intergenic 35.0%
TCGAATGGAATTCACTTACA+AGG + chr4_1:50105899-50105918 Msa0553180:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG + chr4_1:50106053-50106072 Msa0553180:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG + chr4_1:50106069-50106088 Msa0553180:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG + chr4_1:50106171-50106190 Msa0553180:CDS 35.0%
AACCTCATTGGAGAGATTTA+GGG + chr4_1:50106210-50106229 Msa0553180:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG + chr4_1:50106276-50106295 Msa0553180:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG + chr4_1:50106281-50106300 Msa0553180:CDS 35.0%
TTGTGAGGCATATAGAATGA+AGG + chr4_1:50106414-50106433 Msa0553180:CDS 35.0%
CCAAGCTGATATTGAAAACA+AGG - chr4_1:50106479-50106498 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG + chr4_1:50106476-50106495 Msa0553180:CDS 35.0%
AGTAAGACGATGTGATTGTT+TGG + chr4_1:50106509-50106528 Msa0553180:exon 35.0%
ACTTGAGTTTGGCAAAATTG+CGG + chr4_1:50106538-50106557 Msa0553180:three_prime_UTR 35.0%
! AGAGTGTGAGTTTGAACTTT+TGG - chr4_1:50104589-50104608 None:intergenic 35.0%
TTACTTTGTTCTGTTGTTGG+TGG - chr4_1:50104625-50104644 None:intergenic 35.0%
! GAGTTACTTTGTTCTGTTGT+TGG - chr4_1:50104628-50104647 None:intergenic 35.0%
TTCAACTTCAAAACCCATCA+AGG + chr4_1:50104724-50104743 Msa0553180:five_prime_UTR 35.0%
CAAGTATGCAATTCCTTGAT+GGG - chr4_1:50104740-50104759 None:intergenic 35.0%
ACAAGTATGCAATTCCTTGA+TGG - chr4_1:50104741-50104760 None:intergenic 35.0%
AGCTGTTACAAGAAGAAAAG+AGG - chr4_1:50104793-50104812 None:intergenic 35.0%
! ACCGACTCATCAAAAAGTTA+TGG - chr4_1:50104836-50104855 None:intergenic 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG - chr4_1:50105187-50105206 None:intergenic 35.0%
CAAGCATGGAAATTTGATCA+AGG - chr4_1:50105298-50105317 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG + chr4_1:50105375-50105394 Msa0553180:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG + chr4_1:50105499-50105518 Msa0553180:CDS 35.0%
CTATCATTGAAGCAATGGAA+GGG + chr4_1:50105522-50105541 Msa0553180:CDS 35.0%
TATCATTGAAGCAATGGAAG+GGG + chr4_1:50105523-50105542 Msa0553180:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG - chr4_1:50105734-50105753 None:intergenic 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG - chr4_1:50105749-50105768 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG - chr4_1:50105772-50105791 None:intergenic 35.0%
GAGAAACTTCGTGTTAAAAC+CGG + chr4_1:50105779-50105798 Msa0553180:CDS 35.0%
AGAAACTTCGTGTTAAAACC+GGG + chr4_1:50105780-50105799 Msa0553180:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG - chr4_1:50105900-50105919 None:intergenic 35.0%
TCGAATGGAATTCACTTACA+AGG + chr4_1:50105899-50105918 Msa0553180:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG + chr4_1:50106053-50106072 Msa0553180:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG + chr4_1:50106069-50106088 Msa0553180:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG + chr4_1:50106171-50106190 Msa0553180:CDS 35.0%
AACCTCATTGGAGAGATTTA+GGG + chr4_1:50106210-50106229 Msa0553180:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG + chr4_1:50106276-50106295 Msa0553180:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG + chr4_1:50106281-50106300 Msa0553180:CDS 35.0%
TTGTGAGGCATATAGAATGA+AGG + chr4_1:50106414-50106433 Msa0553180:CDS 35.0%
CCAAGCTGATATTGAAAACA+AGG - chr4_1:50106479-50106498 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG + chr4_1:50106476-50106495 Msa0553180:CDS 35.0%
AGTAAGACGATGTGATTGTT+TGG + chr4_1:50106509-50106528 Msa0553180:exon 35.0%
ACTTGAGTTTGGCAAAATTG+CGG + chr4_1:50106538-50106557 Msa0553180:three_prime_UTR 35.0%
CATCACTCTTCAAATCCAAG+AGG + chr4_1:50104661-50104680 Msa0553180:five_prime_UTR 40.0%
AAATTCCAAAGCTTGCCTCT+TGG - chr4_1:50104679-50104698 None:intergenic 40.0%
GTACAGCTATGTTGCAAGAA+GGG + chr4_1:50105087-50105106 Msa0553180:intron 40.0%
CATCATGGAGAAGAGCTTTA+AGG - chr4_1:50105135-50105154 None:intergenic 40.0%
GATTTCAGTTCCTTAAGCCA+AGG - chr4_1:50105188-50105207 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG + chr4_1:50105190-50105209 Msa0553180:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG + chr4_1:50105195-50105214 Msa0553180:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG + chr4_1:50105196-50105215 Msa0553180:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG + chr4_1:50105197-50105216 Msa0553180:CDS 40.0%
TTGCACAAGTGAGCAACAAA+TGG - chr4_1:50105235-50105254 None:intergenic 40.0%
TATCCTATCAGCAAGTGCTT+CGG - chr4_1:50105366-50105385 None:intergenic 40.0%
TCAGGCTATCATTGAAGCAA+TGG + chr4_1:50105517-50105536 Msa0553180:CDS 40.0%
GCTATCATTGAAGCAATGGA+AGG + chr4_1:50105521-50105540 Msa0553180:CDS 40.0%
AGAAATGAAATCCACTGAGC+AGG - chr4_1:50105587-50105606 None:intergenic 40.0%
TGCTGACAACAGGATTGAAT+TGG - chr4_1:50105739-50105758 None:intergenic 40.0%
CAATCCTGTTGTCAGCAAAT+TGG + chr4_1:50105742-50105761 Msa0553180:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG + chr4_1:50105781-50105800 Msa0553180:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG - chr4_1:50105832-50105851 None:intergenic 40.0%
! AATTGCATACCCTTTTAGCC+TGG + chr4_1:50105831-50105850 Msa0553180:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG + chr4_1:50105832-50105851 Msa0553180:CDS 40.0%
TTCATCATCCCAGGCTAAAA+GGG - chr4_1:50105843-50105862 None:intergenic 40.0%
TTTCATCATCCCAGGCTAAA+AGG - chr4_1:50105844-50105863 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG - chr4_1:50105990-50106009 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG + chr4_1:50105997-50106016 Msa0553180:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG + chr4_1:50106075-50106094 Msa0553180:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG - chr4_1:50106088-50106107 None:intergenic 40.0%
ATCTCTCCAATGAGGTTCTT+GGG - chr4_1:50106207-50106226 None:intergenic 40.0%
AATCTCTCCAATGAGGTTCT+TGG - chr4_1:50106208-50106227 None:intergenic 40.0%
GAACCTCATTGGAGAGATTT+AGG + chr4_1:50106209-50106228 Msa0553180:CDS 40.0%
! AGGGTGGAGAAACTTCTTTT+TGG + chr4_1:50106229-50106248 Msa0553180:CDS 40.0%
!! GGAGAAACTTCTTTTTGGTG+AGG + chr4_1:50106234-50106253 Msa0553180:CDS 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG + chr4_1:50106294-50106313 Msa0553180:CDS 40.0%
TTGGTAAGTTGCAAGCAAAG+CGG + chr4_1:50106374-50106393 Msa0553180:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG + chr4_1:50106417-50106436 Msa0553180:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG + chr4_1:50106452-50106471 Msa0553180:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG + chr4_1:50106485-50106504 Msa0553180:CDS 40.0%
CAGCGAGATTGACAAAATCA+CGG - chr4_1:50106571-50106590 None:intergenic 40.0%
CATCACTCTTCAAATCCAAG+AGG + chr4_1:50104661-50104680 Msa0553180:five_prime_UTR 40.0%
AAATTCCAAAGCTTGCCTCT+TGG - chr4_1:50104679-50104698 None:intergenic 40.0%
GTACAGCTATGTTGCAAGAA+GGG + chr4_1:50105087-50105106 Msa0553180:intron 40.0%
CATCATGGAGAAGAGCTTTA+AGG - chr4_1:50105135-50105154 None:intergenic 40.0%
GATTTCAGTTCCTTAAGCCA+AGG - chr4_1:50105188-50105207 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG + chr4_1:50105190-50105209 Msa0553180:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG + chr4_1:50105195-50105214 Msa0553180:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG + chr4_1:50105196-50105215 Msa0553180:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG + chr4_1:50105197-50105216 Msa0553180:CDS 40.0%
TTGCACAAGTGAGCAACAAA+TGG - chr4_1:50105235-50105254 None:intergenic 40.0%
TATCCTATCAGCAAGTGCTT+CGG - chr4_1:50105366-50105385 None:intergenic 40.0%
TCAGGCTATCATTGAAGCAA+TGG + chr4_1:50105517-50105536 Msa0553180:CDS 40.0%
GCTATCATTGAAGCAATGGA+AGG + chr4_1:50105521-50105540 Msa0553180:CDS 40.0%
AGAAATGAAATCCACTGAGC+AGG - chr4_1:50105587-50105606 None:intergenic 40.0%
TGCTGACAACAGGATTGAAT+TGG - chr4_1:50105739-50105758 None:intergenic 40.0%
CAATCCTGTTGTCAGCAAAT+TGG + chr4_1:50105742-50105761 Msa0553180:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG + chr4_1:50105781-50105800 Msa0553180:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG - chr4_1:50105832-50105851 None:intergenic 40.0%
! AATTGCATACCCTTTTAGCC+TGG + chr4_1:50105831-50105850 Msa0553180:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG + chr4_1:50105832-50105851 Msa0553180:CDS 40.0%
TTCATCATCCCAGGCTAAAA+GGG - chr4_1:50105843-50105862 None:intergenic 40.0%
TTTCATCATCCCAGGCTAAA+AGG - chr4_1:50105844-50105863 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG - chr4_1:50105990-50106009 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG + chr4_1:50105997-50106016 Msa0553180:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG + chr4_1:50106075-50106094 Msa0553180:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG - chr4_1:50106088-50106107 None:intergenic 40.0%
ATCTCTCCAATGAGGTTCTT+GGG - chr4_1:50106207-50106226 None:intergenic 40.0%
AATCTCTCCAATGAGGTTCT+TGG - chr4_1:50106208-50106227 None:intergenic 40.0%
GAACCTCATTGGAGAGATTT+AGG + chr4_1:50106209-50106228 Msa0553180:CDS 40.0%
! AGGGTGGAGAAACTTCTTTT+TGG + chr4_1:50106229-50106248 Msa0553180:CDS 40.0%
!! GGAGAAACTTCTTTTTGGTG+AGG + chr4_1:50106234-50106253 Msa0553180:CDS 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG + chr4_1:50106294-50106313 Msa0553180:CDS 40.0%
TTGGTAAGTTGCAAGCAAAG+CGG + chr4_1:50106374-50106393 Msa0553180:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG + chr4_1:50106417-50106436 Msa0553180:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG + chr4_1:50106452-50106471 Msa0553180:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG + chr4_1:50106485-50106504 Msa0553180:CDS 40.0%
CAGCGAGATTGACAAAATCA+CGG - chr4_1:50106571-50106590 None:intergenic 40.0%
CAAATCCAAGAGGCAAGCTT+TGG + chr4_1:50104671-50104690 Msa0553180:five_prime_UTR 45.0%
GGTACAGCTATGTTGCAAGA+AGG + chr4_1:50105086-50105105 Msa0553180:intron 45.0%
! TTACCGAAGCACTTGCTGAT+AGG + chr4_1:50105360-50105379 Msa0553180:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG + chr4_1:50105380-50105399 Msa0553180:CDS 45.0%
CAACTCTACTCGAGTGTCAA+TGG + chr4_1:50105418-50105437 Msa0553180:CDS 45.0%
CAGGATGAGCACTCAAAACT+TGG - chr4_1:50105610-50105629 None:intergenic 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG + chr4_1:50105611-50105630 Msa0553180:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG - chr4_1:50105637-50105656 None:intergenic 45.0%
CCTCCACATTTGAGAATCAC+CGG + chr4_1:50105635-50105654 Msa0553180:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG - chr4_1:50105657-50105676 None:intergenic 45.0%
CAATCAGTCTATGAGCTACC+TGG - chr4_1:50105688-50105707 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG - chr4_1:50105852-50105871 None:intergenic 45.0%
ATGTGCTTCGGTTTACTGGA+TGG - chr4_1:50105928-50105947 None:intergenic 45.0%
CCAGTAAACCGAAGCACATT+CGG + chr4_1:50105929-50105948 Msa0553180:CDS 45.0%
GCAAATTGCCGAATGTGCTT+CGG - chr4_1:50105940-50105959 None:intergenic 45.0%
! GGTGATGATGAACTTGAGTC+AGG - chr4_1:50105998-50106017 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG + chr4_1:50106125-50106144 Msa0553180:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG - chr4_1:50106147-50106166 None:intergenic 45.0%
AACGATCCCAAGAACCTCAT+TGG + chr4_1:50106198-50106217 Msa0553180:CDS 45.0%
CACCCTAAATCTCTCCAATG+AGG - chr4_1:50106215-50106234 None:intergenic 45.0%
CTCATTGGAGAGATTTAGGG+TGG + chr4_1:50106213-50106232 Msa0553180:CDS 45.0%
GGCATGAAAAGCTGGATAAG+TGG + chr4_1:50106302-50106321 Msa0553180:CDS 45.0%
AAGCGGTTCATGCAGAGTTA+TGG + chr4_1:50106391-50106410 Msa0553180:CDS 45.0%
CATGCAGAGTTATGGTTGTG+AGG + chr4_1:50106399-50106418 Msa0553180:CDS 45.0%
ATGGCTGTGTGCTAATATGC+TGG + chr4_1:50106443-50106462 Msa0553180:CDS 45.0%
TTTGGACGAGCACTTGAGTT+TGG + chr4_1:50106527-50106546 Msa0553180:three_prime_UTR 45.0%
TGAGTTTGGCAAAATTGCGG+TGG + chr4_1:50106541-50106560 Msa0553180:three_prime_UTR 45.0%
! ATGGCTTATGCTTTTGCGCA+TGG - chr4_1:50106603-50106622 None:intergenic 45.0%
CAAATCCAAGAGGCAAGCTT+TGG + chr4_1:50104671-50104690 Msa0553180:five_prime_UTR 45.0%
GGTACAGCTATGTTGCAAGA+AGG + chr4_1:50105086-50105105 Msa0553180:intron 45.0%
! TTACCGAAGCACTTGCTGAT+AGG + chr4_1:50105360-50105379 Msa0553180:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG + chr4_1:50105380-50105399 Msa0553180:CDS 45.0%
CAACTCTACTCGAGTGTCAA+TGG + chr4_1:50105418-50105437 Msa0553180:CDS 45.0%
CAGGATGAGCACTCAAAACT+TGG - chr4_1:50105610-50105629 None:intergenic 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG + chr4_1:50105611-50105630 Msa0553180:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG - chr4_1:50105637-50105656 None:intergenic 45.0%
CCTCCACATTTGAGAATCAC+CGG + chr4_1:50105635-50105654 Msa0553180:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG - chr4_1:50105657-50105676 None:intergenic 45.0%
CAATCAGTCTATGAGCTACC+TGG - chr4_1:50105688-50105707 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG - chr4_1:50105852-50105871 None:intergenic 45.0%
ATGTGCTTCGGTTTACTGGA+TGG - chr4_1:50105928-50105947 None:intergenic 45.0%
CCAGTAAACCGAAGCACATT+CGG + chr4_1:50105929-50105948 Msa0553180:CDS 45.0%
GCAAATTGCCGAATGTGCTT+CGG - chr4_1:50105940-50105959 None:intergenic 45.0%
! GGTGATGATGAACTTGAGTC+AGG - chr4_1:50105998-50106017 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG + chr4_1:50106125-50106144 Msa0553180:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG - chr4_1:50106147-50106166 None:intergenic 45.0%
AACGATCCCAAGAACCTCAT+TGG + chr4_1:50106198-50106217 Msa0553180:CDS 45.0%
CACCCTAAATCTCTCCAATG+AGG - chr4_1:50106215-50106234 None:intergenic 45.0%
CTCATTGGAGAGATTTAGGG+TGG + chr4_1:50106213-50106232 Msa0553180:CDS 45.0%
GGCATGAAAAGCTGGATAAG+TGG + chr4_1:50106302-50106321 Msa0553180:CDS 45.0%
AAGCGGTTCATGCAGAGTTA+TGG + chr4_1:50106391-50106410 Msa0553180:CDS 45.0%
CATGCAGAGTTATGGTTGTG+AGG + chr4_1:50106399-50106418 Msa0553180:CDS 45.0%
ATGGCTGTGTGCTAATATGC+TGG + chr4_1:50106443-50106462 Msa0553180:CDS 45.0%
TTTGGACGAGCACTTGAGTT+TGG + chr4_1:50106527-50106546 Msa0553180:three_prime_UTR 45.0%
TGAGTTTGGCAAAATTGCGG+TGG + chr4_1:50106541-50106560 Msa0553180:three_prime_UTR 45.0%
! ATGGCTTATGCTTTTGCGCA+TGG - chr4_1:50106603-50106622 None:intergenic 45.0%
TCCATGATGCCACCTTCATC+TGG + chr4_1:50105146-50105165 Msa0553180:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG + chr4_1:50105152-50105171 Msa0553180:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG - chr4_1:50105158-50105177 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG - chr4_1:50105161-50105180 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG + chr4_1:50105175-50105194 Msa0553180:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG - chr4_1:50105182-50105201 None:intergenic 50.0%
CTGCATAGTATCACCGTCAG+GGG - chr4_1:50105324-50105343 None:intergenic 50.0%
GCTGCATAGTATCACCGTCA+GGG - chr4_1:50105325-50105344 None:intergenic 50.0%
CTGACGGTGATACTATGCAG+CGG + chr4_1:50105324-50105343 Msa0553180:CDS 50.0%
TTGAGCGCTTTGTGGATTCC+AGG - chr4_1:50105401-50105420 None:intergenic 50.0%
GAGTAGAGTTGAGCGCTTTG+TGG - chr4_1:50105409-50105428 None:intergenic 50.0%
CCGGTGATTCTCAAATGTGG+AGG - chr4_1:50105638-50105657 None:intergenic 50.0%
ACGCCGGTGATTCTCAAATG+TGG - chr4_1:50105641-50105660 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG + chr4_1:50105655-50105674 Msa0553180:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG + chr4_1:50105667-50105686 Msa0553180:CDS 50.0%
ACTTCGTGTTAAAACCGGGG+AGG + chr4_1:50105784-50105803 Msa0553180:CDS 50.0%
ACTAATTGCTAGTGCCTCCC+CGG - chr4_1:50105801-50105820 None:intergenic 50.0%
CCGAATGTGCTTCGGTTTAC+TGG - chr4_1:50105932-50105951 None:intergenic 50.0%
TCCATGATGCCACCTTCATC+TGG + chr4_1:50105146-50105165 Msa0553180:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG + chr4_1:50105152-50105171 Msa0553180:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG - chr4_1:50105158-50105177 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG - chr4_1:50105161-50105180 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG + chr4_1:50105175-50105194 Msa0553180:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG - chr4_1:50105182-50105201 None:intergenic 50.0%
CTGCATAGTATCACCGTCAG+GGG - chr4_1:50105324-50105343 None:intergenic 50.0%
GCTGCATAGTATCACCGTCA+GGG - chr4_1:50105325-50105344 None:intergenic 50.0%
CTGACGGTGATACTATGCAG+CGG + chr4_1:50105324-50105343 Msa0553180:CDS 50.0%
TTGAGCGCTTTGTGGATTCC+AGG - chr4_1:50105401-50105420 None:intergenic 50.0%
GAGTAGAGTTGAGCGCTTTG+TGG - chr4_1:50105409-50105428 None:intergenic 50.0%
CCGGTGATTCTCAAATGTGG+AGG - chr4_1:50105638-50105657 None:intergenic 50.0%
ACGCCGGTGATTCTCAAATG+TGG - chr4_1:50105641-50105660 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG + chr4_1:50105655-50105674 Msa0553180:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG + chr4_1:50105667-50105686 Msa0553180:CDS 50.0%
ACTTCGTGTTAAAACCGGGG+AGG + chr4_1:50105784-50105803 Msa0553180:CDS 50.0%
ACTAATTGCTAGTGCCTCCC+CGG - chr4_1:50105801-50105820 None:intergenic 50.0%
CCGAATGTGCTTCGGTTTAC+TGG - chr4_1:50105932-50105951 None:intergenic 50.0%
GCCAGATGAAGGTGGCATCA+TGG - chr4_1:50105150-50105169 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG + chr4_1:50105168-50105187 Msa0553180:CDS 55.0%
TCCATGCTTGCTTCCCCTGA+CGG + chr4_1:50105308-50105327 Msa0553180:CDS 55.0%
CGCTGCATAGTATCACCGTC+AGG - chr4_1:50105326-50105345 None:intergenic 55.0%
ATGCTGCTGAACCTGCTCAG+TGG + chr4_1:50105573-50105592 Msa0553180:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG - chr4_1:50105629-50105648 None:intergenic 55.0%
GCCAGATGAAGGTGGCATCA+TGG - chr4_1:50105150-50105169 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG + chr4_1:50105168-50105187 Msa0553180:CDS 55.0%
TCCATGCTTGCTTCCCCTGA+CGG + chr4_1:50105308-50105327 Msa0553180:CDS 55.0%
CGCTGCATAGTATCACCGTC+AGG - chr4_1:50105326-50105345 None:intergenic 55.0%
ATGCTGCTGAACCTGCTCAG+TGG + chr4_1:50105573-50105592 Msa0553180:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG - chr4_1:50105629-50105648 None:intergenic 55.0%
ACCGTCAGGGGAAGCAAGCA+TGG - chr4_1:50105312-50105331 None:intergenic 60.0%
ACCGTCAGGGGAAGCAAGCA+TGG - chr4_1:50105312-50105331 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 50104578 50106776 50104578 ID=Msa0553180;Name=Msa0553180
chr4_1 mRNA 50104578 50106776 50104578 ID=Msa0553180-mRNA-1;Parent=Msa0553180;Name=Msa0553180-mRNA-1;_AED=0.00;_eAED=0.00;_QI=196|0|0.5|1|0|1|2|263|475
chr4_1 exon 50104578 50104782 50104578 ID=Msa0553180-mRNA-1:exon:14144;Parent=Msa0553180-mRNA-1
chr4_1 exon 50105095 50106776 50105095 ID=Msa0553180-mRNA-1:exon:14145;Parent=Msa0553180-mRNA-1
chr4_1 five_prime_UTR 50104578 50104773 50104578 ID=Msa0553180-mRNA-1:five_prime_utr;Parent=Msa0553180-mRNA-1
chr4_1 CDS 50104774 50104782 50104774 ID=Msa0553180-mRNA-1:cds;Parent=Msa0553180-mRNA-1
chr4_1 CDS 50105095 50106513 50105095 ID=Msa0553180-mRNA-1:cds;Parent=Msa0553180-mRNA-1
chr4_1 three_prime_UTR 50106514 50106776 50106514 ID=Msa0553180-mRNA-1:three_prime_utr;Parent=Msa0553180-mRNA-1
Gene Sequence

>Msa0553180

ATGATTTTGATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCTGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAATCATGTAGCTGCTTGTAATCTTGAGCATGCAAATTCAACCCTTGATCAAATTTCCATGCTTGCTTCCCCTGACGGTGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCGCTCAACTCTACTCGAGTGTCAATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATCATTGAAGCAATGGAAGGGGAGAGAATGATTCACATAATTGATCTCAATGCTGCTGAACCTGCTCAGTGGATTTCATTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCCCTCCACATTTGAGAATCACCGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCACATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGTTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGATCCCAAGAACCTCATTGGAGAGATTTAGGGTGGAGAAACTTCTTTTTGGTGAGGAAATCAAGAATATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGCGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGCTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA
Protein sequence

>Msa0553180

MILMLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANSTLDQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNAAEPAQWISFLQVLSAHPDGPPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSTFGNLLEKDLVSSYTISPDSSSSSPVSLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTIPRTSLERFRVEKLLFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVLICWQDRSLFSISAWRSRK*