AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0690290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0690290 MtrunA17_Chr4g0038851 95.975 472 19 0 4 475 1 472 0.0 917
Msa0690290 MtrunA17_Chr5g0396491 74.739 479 113 6 4 475 4 481 0.0 731
Msa0690290 MtrunA17_Chr3g0085681 49.888 445 213 4 30 474 4 438 7.35e-161 462
Msa0690290 MtrunA17_Chr3g0085361 50.986 355 163 5 120 474 9 352 3.34e-126 370
Msa0690290 MtrunA17_Chr7g0224271 33.778 450 216 6 40 472 289 673 4.16e-74 246
Msa0690290 MtrunA17_Chr5g0447691 31.323 431 230 6 48 472 174 544 3.52e-66 222
Msa0690290 MtrunA17_Chr1g0159461 29.837 429 235 8 50 472 224 592 8.20e-56 195
Msa0690290 MtrunA17_Chr3g0125811 29.138 429 238 8 50 472 202 570 4.44e-54 190
Msa0690290 MtrunA17_Chr3g0101141 27.928 444 253 8 35 472 160 542 2.33e-52 185
Msa0690290 MtrunA17_Chr8g0357601 29.955 444 230 12 44 472 175 552 6.94e-51 181
Msa0690290 MtrunA17_Chr4g0031721 28.438 429 238 7 51 471 316 683 8.54e-50 180
Msa0690290 MtrunA17_Chr4g0057891 28.798 441 238 11 45 472 143 520 5.29e-49 175
Msa0690290 MtrunA17_Chr2g0306641 30.455 440 231 15 42 473 116 488 9.28e-48 172
Msa0690290 MtrunA17_Chr4g0031701 26.490 453 264 7 24 467 229 621 4.41e-47 172
Msa0690290 MtrunA17_Chr2g0290241 28.037 428 241 9 52 472 58 425 4.69e-47 168
Msa0690290 MtrunA17_Chr7g0238891 27.103 428 247 5 51 472 372 740 1.66e-46 172
Msa0690290 MtrunA17_Chr4g0031731 26.269 453 265 7 24 467 229 621 3.46e-45 167
Msa0690290 MtrunA17_Chr2g0328391 26.168 428 251 5 51 471 299 668 3.67e-44 164
Msa0690290 MtrunA17_Chr4g0031711 27.174 460 261 11 23 471 336 732 4.35e-44 165
Msa0690290 MtrunA17_Chr2g0328411 26.606 436 234 8 51 471 289 653 5.84e-41 155
Msa0690290 MtrunA17_Chr2g0328431 26.464 427 246 7 51 471 289 653 2.05e-40 154
Msa0690290 MtrunA17_Chr4g0057291 35.349 215 135 1 44 254 285 499 3.22e-38 147
Msa0690290 MtrunA17_Chr4g0057291 31.373 153 98 5 325 471 510 661 2.66e-18 87.8
Msa0690290 MtrunA17_Chr2g0328381 25.172 437 262 8 40 471 264 640 3.81e-38 147
Msa0690290 MtrunA17_Chr2g0294471 29.306 447 235 15 42 472 195 576 6.59e-38 145
Msa0690290 MtrunA17_Chr1g0200401 27.948 458 230 14 45 473 74 460 1.14e-37 143
Msa0690290 MtrunA17_Chr7g0245601 40.314 191 111 2 55 244 442 630 3.17e-37 145
Msa0690290 MtrunA17_Chr7g0245601 36.913 149 89 3 327 472 646 792 5.24e-20 93.6
Msa0690290 MtrunA17_Chr1g0183641 27.689 437 239 12 43 474 115 479 5.57e-37 142
Msa0690290 MtrunA17_Chr2g0294461 27.938 451 239 14 42 472 201 585 9.94e-37 142
Msa0690290 MtrunA17_Chr2g0328401 26.984 378 208 6 51 422 290 605 4.99e-36 141
Msa0690290 MtrunA17_Chr3g0110971 34.685 222 131 4 39 255 207 419 1.47e-34 136
Msa0690290 MtrunA17_Chr3g0110971 36.196 163 91 2 323 472 425 587 4.70e-28 117
Msa0690290 MtrunA17_Chr8g0376381 33.482 224 130 5 39 255 157 368 1.22e-31 127
Msa0690290 MtrunA17_Chr8g0376381 36.601 153 90 3 323 472 374 522 3.35e-28 117
Msa0690290 MtrunA17_Chr3g0114841 25.708 459 250 14 38 471 107 499 7.45e-31 125
Msa0690290 MtrunA17_Chr2g0319091 33.778 225 139 3 48 266 209 429 1.25e-30 125
Msa0690290 MtrunA17_Chr2g0319091 41.611 149 86 1 323 471 431 578 2.46e-29 121
Msa0690290 MtrunA17_Chr5g0445331 31.556 225 145 3 35 251 146 369 7.93e-30 122
Msa0690290 MtrunA17_Chr5g0445331 38.298 141 86 1 332 472 393 532 1.24e-25 109
Msa0690290 MtrunA17_Chr2g0328361 24.541 436 241 10 40 471 263 614 8.86e-30 122
Msa0690290 MtrunA17_Chr2g0328341 28.352 261 171 5 26 278 251 503 1.44e-29 122
Msa0690290 MtrunA17_Chr4g0031741 31.111 225 142 4 48 266 281 498 3.06e-29 121
Msa0690290 MtrunA17_Chr4g0031741 32.000 150 101 1 323 471 500 649 4.18e-16 81.3
Msa0690290 MtrunA17_Chr7g0270031 25.567 485 278 13 4 472 137 554 7.56e-29 119
Msa0690290 MtrunA17_Chr4g0038761 32.124 193 128 2 55 244 88 280 3.93e-26 110
Msa0690290 MtrunA17_Chr4g0038761 39.597 149 84 4 326 472 298 442 8.02e-20 91.7
Msa0690290 MtrunA17_Chr2g0328371 25.000 292 158 7 134 422 13 246 8.50e-26 105
Msa0690290 MtrunA17_Chr1g0207361 63.366 101 35 2 276 375 64 163 2.07e-25 102
Msa0690290 MtrunA17_Chr1g0207361 72.222 54 15 0 85 138 1 54 7.67e-22 92.4
Msa0690290 MtrunA17_Chr4g0053901 24.187 492 293 11 14 471 22 467 2.75e-25 108
Msa0690290 MtrunA17_Chr5g0401421 24.330 485 284 14 22 471 9 445 4.87e-25 107
Msa0690290 MtrunA17_Chr5g0424131 31.169 231 142 4 37 250 107 337 6.60e-25 107
Msa0690290 MtrunA17_Chr5g0424131 26.667 150 107 3 324 471 350 498 3.80e-11 65.1
Msa0690290 MtrunA17_Chr5g0401131 31.651 218 139 4 45 253 108 324 1.69e-24 106
Msa0690290 MtrunA17_Chr4g0076091 31.163 215 140 3 46 253 170 383 2.23e-24 106
Msa0690290 MtrunA17_Chr4g0076091 34.228 149 97 1 323 471 395 542 1.48e-21 97.8
Msa0690290 MtrunA17_Chr2g0329591 31.019 216 137 5 42 247 65 278 5.16e-22 98.6
Msa0690290 MtrunA17_Chr1g0194621 33.728 169 102 5 310 472 354 518 2.40e-21 97.1
Msa0690290 MtrunA17_Chr1g0194621 25.000 248 165 8 42 269 137 383 5.65e-12 67.8
Msa0690290 MtrunA17_Chr4g0039661 28.700 223 149 3 48 263 190 409 6.84e-20 92.4
Msa0690290 MtrunA17_Chr4g0039661 30.055 183 115 6 298 472 378 555 1.75e-13 72.8
Msa0690290 MtrunA17_Chr6g0468561 26.331 357 191 11 127 472 367 662 1.43e-19 92.0
Msa0690290 MtrunA17_Chr4g0012071 25.059 427 242 11 52 472 197 551 1.68e-19 91.3
Msa0690290 MtrunA17_Chr7g0236851 33.333 150 95 3 323 471 511 656 2.94e-19 90.9
Msa0690290 MtrunA17_Chr7g0230731 32.941 170 106 3 22 191 349 510 1.42e-14 76.3
Msa0690290 MtrunA17_Chr7g0230771 32.941 170 106 3 22 191 352 513 1.62e-14 76.3
Msa0690290 MtrunA17_Chr8g0383981 29.126 206 132 8 48 248 136 332 1.42e-13 72.8
Msa0690290 MtrunA17_Chr3g0087711 51.724 58 28 0 202 259 1 58 8.09e-13 63.2
Msa0690290 MtrunA17_Chr2g0323111 26.174 149 108 2 323 471 159 305 2.17e-12 68.2
Msa0690290 MtrunA17_Chr8g0344101 22.414 464 250 14 45 475 217 603 6.48e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0690290 AT1G50420.1 62.603 484 170 5 1 475 1 482 0.0 612
Msa0690290 AT1G66350.1 32.037 437 221 8 39 472 143 506 5.51e-68 226
Msa0690290 AT5G48150.1 30.679 427 231 5 52 472 123 490 5.85e-66 220
Msa0690290 AT5G48150.4 30.679 427 231 5 52 472 123 490 5.85e-66 220
Msa0690290 AT5G48150.2 30.679 427 231 5 52 472 123 490 5.85e-66 220
Msa0690290 AT5G48150.3 30.679 427 231 5 52 472 123 490 5.85e-66 220
Msa0690290 AT2G04890.1 32.938 422 222 5 51 472 53 413 2.62e-65 216
Msa0690290 AT5G17490.1 30.905 453 229 12 34 472 134 516 1.30e-59 204
Msa0690290 AT1G50600.3 29.322 457 251 10 27 472 131 526 1.90e-58 201
Msa0690290 AT1G50600.1 29.386 456 250 10 28 472 203 597 4.25e-58 202
Msa0690290 AT1G50600.2 29.322 457 251 10 27 472 206 601 4.79e-58 202
Msa0690290 AT1G21450.1 30.769 429 232 7 50 472 224 593 6.82e-56 196
Msa0690290 AT1G14920.1 30.112 445 225 12 39 472 160 529 5.52e-53 187
Msa0690290 AT2G01570.1 28.764 445 231 13 39 472 212 581 1.11e-47 173
Msa0690290 AT5G41920.1 30.023 433 227 11 48 474 40 402 9.05e-46 164
Msa0690290 AT1G07530.1 27.955 440 227 10 51 471 396 764 5.73e-44 165
Msa0690290 AT3G54220.1 42.180 211 120 1 36 244 278 488 5.17e-41 155
Msa0690290 AT3G54220.1 35.616 146 92 2 329 474 509 652 7.16e-20 93.2
Msa0690290 AT2G29060.1 26.267 434 250 8 48 471 318 691 6.17e-40 153
Msa0690290 AT2G29060.2 27.189 434 246 10 48 471 281 654 7.30e-40 152
Msa0690290 AT1G07520.2 25.282 443 263 7 40 471 143 528 4.61e-39 149
Msa0690290 AT1G07520.4 25.282 443 263 7 40 471 143 528 4.61e-39 149
Msa0690290 AT5G66770.1 28.899 436 231 12 48 472 217 584 8.05e-39 149
Msa0690290 AT1G07520.3 25.282 443 263 7 40 471 308 693 2.22e-38 149
Msa0690290 AT1G07520.1 25.282 443 263 7 40 471 307 692 2.55e-38 148
Msa0690290 AT5G59450.1 25.806 434 254 7 48 472 224 598 1.56e-37 145
Msa0690290 AT2G29065.1 25.571 438 247 10 51 471 252 627 9.41e-37 143
Msa0690290 AT3G46600.2 25.926 432 254 6 50 475 3 374 2.70e-36 138
Msa0690290 AT2G37650.2 24.826 431 257 6 48 472 332 701 5.39e-36 142
Msa0690290 AT2G37650.1 24.826 431 257 6 48 472 344 713 5.99e-36 141
Msa0690290 AT3G46600.3 25.926 432 254 6 50 475 179 550 6.07e-35 137
Msa0690290 AT3G46600.1 25.843 445 263 7 37 475 199 582 1.57e-34 136
Msa0690290 AT1G63100.2 32.218 239 157 4 43 277 269 506 2.10e-31 128
Msa0690290 AT1G63100.2 33.125 160 94 2 325 471 494 653 1.50e-20 95.5
Msa0690290 AT1G63100.1 32.218 239 157 4 43 277 244 481 2.91e-31 127
Msa0690290 AT1G63100.1 33.125 160 94 2 325 471 469 628 1.03e-20 95.9
Msa0690290 AT3G03450.1 31.839 223 143 5 39 255 171 390 2.63e-30 124
Msa0690290 AT3G03450.1 38.854 157 94 2 317 472 390 545 1.07e-28 119
Msa0690290 AT5G52510.1 26.376 436 246 13 50 472 267 640 3.05e-30 124
Msa0690290 AT1G55580.1 38.255 149 91 1 325 472 297 445 4.39e-28 116
Msa0690290 AT1G55580.1 27.843 255 143 8 47 261 40 293 5.29e-13 71.2
Msa0690290 AT4G37650.1 31.984 247 138 7 24 253 131 364 3.06e-24 106
Msa0690290 AT3G50650.1 26.157 432 235 13 56 472 180 542 5.40e-24 105
Msa0690290 AT4G17230.1 34.000 150 98 1 323 472 377 525 6.54e-23 102
Msa0690290 AT4G17230.1 29.245 212 143 2 46 251 153 363 1.62e-22 100
Msa0690290 AT4G17230.2 34.000 150 98 1 323 472 377 525 6.54e-23 102
Msa0690290 AT4G17230.2 29.245 212 143 2 46 251 153 363 1.62e-22 100
Msa0690290 AT4G08250.1 30.116 259 153 9 6 250 72 316 2.81e-21 97.1
Msa0690290 AT3G49950.1 22.126 461 276 14 29 471 12 407 7.07e-16 79.7
Msa0690290 AT4G00150.1 27.179 195 133 5 283 474 368 556 9.47e-12 67.8

Find 127 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCATTCGAGAGATTT+AGG 0.125804 4_4:+57976044 Msa0690290:CDS
CCGAATATGCTTCGGTTTAC+TGG 0.158109 4_4:-57975764 None:intergenic
TTCTTCCTTAGTCATGATTT+TGG 0.181091 4_4:+57974590 Msa0690290:exon
AATGAGCCTCTAAGTTATTT+TGG 0.189632 4_4:+57976190 Msa0690290:CDS
TTTCATCATCCCAGGCTAAA+AGG 0.243724 4_4:-57975676 None:intergenic
TGCAGCACTATTTGATTGTT+TGG 0.256288 4_4:+57976006 Msa0690290:CDS
CAATCCTGTTGTCAGCAAAT+TGG 0.264169 4_4:+57975577 Msa0690290:CDS
CATCATGGAGAAGAGCTTTA+AGG 0.269652 4_4:-57974967 None:intergenic
CAGAACAAAGTAACTCATTA+AGG 0.282442 4_4:+57974462 Msa0690290:five_prime_UTR
AGATTTGATTTAGCTGGATT+TGG 0.318965 4_4:+57976166 Msa0690290:CDS
CAATATCAGCTTGGAGATCT+AGG 0.325494 4_4:+57976320 Msa0690290:CDS
AATCTCTCGAATGAGTTTCT+TGG 0.330855 4_4:-57976040 None:intergenic
AGAGTGTGAGTTTGAACTTT+TGG 0.331382 4_4:-57974416 None:intergenic
ATGCCACCTTCATCTGGCTT+TGG 0.334564 4_4:+57974987 Msa0690290:CDS
CCAGTAAACCGAAGCATATT+CGG 0.339215 4_4:+57975764 Msa0690290:CDS
AGCATTTGTTCTCACAAATC+AGG 0.349043 4_4:+57975334 Msa0690290:CDS
TCCATGATGCCACCTTCATC+TGG 0.349847 4_4:+57974981 Msa0690290:CDS
AACTTACCAAAATAACTTAG+AGG 0.353618 4_4:-57976196 None:intergenic
TTGAGTGCTTTGTGGATTCC+AGG 0.357455 4_4:-57975233 None:intergenic
AAACTCATTCGAGAGATTTA+GGG 0.358753 4_4:+57976045 Msa0690290:CDS
TGCTGACAACAGGATTGAAT+TGG 0.362322 4_4:-57975571 None:intergenic
TTTGGACGAGCACTTGAGTT+TGG 0.364682 4_4:+57976362 Msa0690290:three_prime_UTR
CAATTTGCTTGAGAAAGATT+TGG 0.366215 4_4:+57975787 Msa0690290:CDS
ATCTCCACACCCTTGGCTTA+AGG 0.366272 4_4:+57975010 Msa0690290:CDS
GAGTTACTTTGTTCTGTTGT+TGG 0.378375 4_4:-57974455 None:intergenic
TATAGAATGAAGGAGGAAAA+TGG 0.386592 4_4:+57976259 Msa0690290:CDS
TGAACTTGAGTCAGGACTTA+TGG 0.391992 4_4:-57975822 None:intergenic
ATCTCTCGAATGAGTTTCTT+GGG 0.394420 4_4:-57976039 None:intergenic
ACAAGTATGCAATTCCTTGA+TGG 0.395999 4_4:-57974567 None:intergenic
CAAATCCAAGAGGCAAGCTT+TGG 0.398712 4_4:+57974500 Msa0690290:five_prime_UTR
CTCAAATGTGGAGGGCCATC+AGG 0.400333 4_4:-57975461 None:intergenic
GAGAAACTTCGTGTTAAAAC+CGG 0.400451 4_4:+57975614 Msa0690290:CDS
CAGGATGAGCACTCAAAACT+TGG 0.403034 4_4:-57975442 None:intergenic
TTACCGAAGCACTTGCTGAT+AGG 0.405714 4_4:+57975195 Msa0690290:CDS
CACGAAGTTTCTCAAAATCA+AGG 0.414533 4_4:-57975604 None:intergenic
GCTTTGGATCTCCACACCCT+TGG 0.415132 4_4:+57975003 Msa0690290:CDS
GAGTAGAGTTGAGTGCTTTG+TGG 0.416744 4_4:-57975241 None:intergenic
TTCAATTTCAAAACCCATCA+AGG 0.420097 4_4:+57974553 Msa0690290:five_prime_UTR
TTTGAAGTGTTCAAAGAAAC+CGG 0.422703 4_4:-57975851 None:intergenic
AGTAAGACGATGTGATTGTT+TGG 0.435895 4_4:+57976344 Msa0690290:exon
TTTATGAGATTTGATTTAGC+TGG 0.436460 4_4:+57976160 Msa0690290:CDS
AGGCTAAAAGGGTATGCAAT+TGG 0.439239 4_4:-57975664 None:intergenic
AGGGTGGAGAAACTTCATTT+TGG 0.439547 4_4:+57976064 Msa0690290:CDS
AAGAGGTTCATGCAGAGTTA+TGG 0.445441 4_4:+57976226 Msa0690290:CDS
CGCTGCATAGTATCGCCGTC+AGG 0.446613 4_4:-57975158 None:intergenic
CAAGTATGCAATTCCTTGAT+GGG 0.447226 4_4:-57974566 None:intergenic
GGAACTGAAATCTGAGGAAA+GGG 0.447352 4_4:+57975031 Msa0690290:CDS
CTTCATGGTTTCATCATCCC+AGG 0.449792 4_4:-57975684 None:intergenic
GAACAAGACTCTAATCATAA+TGG 0.450476 4_4:+57975941 Msa0690290:CDS
TGCTAATATGCTGGCAAGAT+AGG 0.451606 4_4:+57976287 Msa0690290:CDS
TCTGTTACAACCATCACCTT+TGG 0.451615 4_4:-57975920 None:intergenic
AAATTCCAAAGCTTGCCTCT+TGG 0.452365 4_4:-57974505 None:intergenic
AAAGGAAAGGCATGAAAAGC+TGG 0.456379 4_4:+57976129 Msa0690290:CDS
TTCATCATCCCAGGCTAAAA+GGG 0.472082 4_4:-57975675 None:intergenic
GCAAATTGCCGAATATGCTT+CGG 0.476281 4_4:-57975772 None:intergenic
CAAGATTACAAGCAGCTACA+TGG 0.487046 4_4:-57975091 None:intergenic
TTGCACAAGTGAGCAACAAA+TGG 0.493036 4_4:-57975067 None:intergenic
ATATGCTTCGGTTTACTGGA+TGG 0.504223 4_4:-57975760 None:intergenic
ACAAATGCTACTTTCATGAA+AGG 0.509715 4_4:-57975320 None:intergenic
AGGATACTTAAAGCATGGCC+TGG 0.511478 4_4:+57975215 Msa0690290:CDS
TTGTAAGTGAATTCCATTCG+AGG 0.515092 4_4:-57975732 None:intergenic
CAAATGCTACTTTCATGAAA+GGG 0.516070 4_4:-57975319 None:intergenic
TTACTTTGTTCTGTTGTTGG+TGG 0.520167 4_4:-57974452 None:intergenic
TCAGGCTATTATTGAAGCAA+TGG 0.520201 4_4:+57975352 Msa0690290:CDS
GCCAGATGAAGGTGGCATCA+TGG 0.521665 4_4:-57974982 None:intergenic
TCCATGCTTGCTTCCCCTGA+CGG 0.525920 4_4:+57975143 Msa0690290:CDS
ACATACCAAAATCATGACTA+AGG 0.527098 4_4:-57974595 None:intergenic
CAAGAACATTATTGCATGTG+AGG 0.529680 4_4:+57976096 Msa0690290:CDS
GCCGTCAGGGGAAGCAAGCA+TGG 0.529802 4_4:-57975144 None:intergenic
TCGAATGGAATTCACTTACA+AGG 0.530422 4_4:+57975734 Msa0690290:CDS
AGAAACTTCGTGTTAAAACC+GGG 0.531168 4_4:+57975615 Msa0690290:CDS
GGCATGAAAAGCTGGATAAG+TGG 0.531394 4_4:+57976137 Msa0690290:CDS
GGTGATGATGAACTTGAGTC+AGG 0.534611 4_4:-57975830 None:intergenic
ATGTGAGGGATTAGAAAGAA+AGG 0.534942 4_4:+57976111 Msa0690290:CDS
TATCCTATCAGCAAGTGCTT+CGG 0.537933 4_4:-57975198 None:intergenic
ATTTGATTGTTTGGAATCAA+CGG 0.538609 4_4:+57976015 Msa0690290:CDS
ACTAATTGCTAGTGCCTCCC+CGG 0.543403 4_4:-57975633 None:intergenic
TGACTCAAGTTCATCATCAC+CGG 0.544363 4_4:+57975832 Msa0690290:CDS
AGGAACTGAAATCTGAGGAA+AGG 0.544931 4_4:+57975030 Msa0690290:CDS
GATTTCAGTTCCTTAAGCCA+AGG 0.546043 4_4:-57975020 None:intergenic
TTGTGAGGCATATAGAATGA+AGG 0.546355 4_4:+57976249 Msa0690290:CDS
GTACAGCTATGTTGCAAGAA+GGG 0.546911 4_4:+57974922 Msa0690290:intron
CCAAGCTGATATTGAAAACA+AGG 0.551538 4_4:-57976311 None:intergenic
AGTTCCTTAAGCCAAGGGTG+TGG 0.551766 4_4:-57975014 None:intergenic
AGAAGTGAAATCCACTGAGC+AGG 0.552331 4_4:-57975419 None:intergenic
ATGGCTGTGTGCTAATATGC+TGG 0.553571 4_4:+57976278 Msa0690290:CDS
CAATCAGTCTATGAGCTACC+TGG 0.553683 4_4:-57975520 None:intergenic
CCTCCACATTTGAGAATCAC+CGG 0.556070 4_4:+57975470 Msa0690290:CDS
GAAGAAAGAGGTTCTCGACC+AGG 0.556759 4_4:+57975502 Msa0690290:CDS
ATTTCAGTTCCTTAAGCCAA+GGG 0.557071 4_4:-57975019 None:intergenic
CTCTTTCTTCTGATGAACGC+CGG 0.562200 4_4:-57975489 None:intergenic
AGTGTAGAGCTTCTAGTAGC+CGG 0.564547 4_4:-57975979 None:intergenic
GCTATTATTGAAGCAATGGA+AGG 0.565201 4_4:+57975356 Msa0690290:CDS
CTCATTCGAGAGATTTAGGG+TGG 0.565611 4_4:+57976048 Msa0690290:CDS
AAGAACATTATTGCATGTGA+GGG 0.567712 4_4:+57976097 Msa0690290:CDS
GGTACAGCTATGTTGCAAGA+AGG 0.572547 4_4:+57974921 Msa0690290:intron
GGAGATCCAAAGCCAGATGA+AGG 0.572774 4_4:-57974993 None:intergenic
GAGTTTATCACCAAAGGTGA+TGG 0.577759 4_4:+57975910 Msa0690290:CDS
ACAACAGGATTGAATTGGAA+TGG 0.580239 4_4:-57975566 None:intergenic
CATGCAGAGTTATGGTTGTG+AGG 0.584714 4_4:+57976234 Msa0690290:CDS
GCTGCATAGTATCGCCGTCA+GGG 0.585579 4_4:-57975157 None:intergenic
TACAAAATACTTTCATACAA+AGG 0.591122 4_4:-57976504 None:intergenic
CAACTCTACTCGAGTGTCGA+TGG 0.592320 4_4:+57975253 Msa0690290:CDS
CGGCGTTCATCAGAAGAAAG+AGG 0.593327 4_4:+57975490 Msa0690290:CDS
ACTTGAGTTTGGCAAAATTG+CGG 0.593940 4_4:+57976373 Msa0690290:three_prime_UTR
TATTATTGAAGCAATGGAAG+GGG 0.594602 4_4:+57975358 Msa0690290:CDS
TTGGTAAGTTGCAAGCAAAG+AGG 0.603635 4_4:+57976209 Msa0690290:CDS
CTGATAGGATACTTAAAGCA+TGG 0.603739 4_4:+57975210 Msa0690290:CDS
AGACATTTCTCAATGCATTG+TGG 0.605127 4_4:+57975888 Msa0690290:CDS
CATCACTCTTCAAATCCAAG+AGG 0.605474 4_4:+57974490 Msa0690290:five_prime_UTR
GCTTAAGGAACTGAAATCTG+AGG 0.605648 4_4:+57975025 Msa0690290:CDS
CTATTATTGAAGCAATGGAA+GGG 0.615371 4_4:+57975357 Msa0690290:CDS
ATTGTGGAGTTTATCACCAA+AGG 0.618172 4_4:+57975904 Msa0690290:CDS
CGGTGATTCTCAAATGTGGA+GGG 0.619559 4_4:-57975469 None:intergenic
TGAGGCATATAGAATGAAGG+AGG 0.622680 4_4:+57976252 Msa0690290:CDS
AGGGATTAGAAAGAAAGGAA+AGG 0.622681 4_4:+57976116 Msa0690290:CDS
ACGCCGGTGATTCTCAAATG+TGG 0.623446 4_4:-57975473 None:intergenic
CTGCATAGTATCGCCGTCAG+GGG 0.626726 4_4:-57975156 None:intergenic
CCGGTGATTCTCAAATGTGG+AGG 0.626889 4_4:-57975470 None:intergenic
TGAGTTTGGCAAAATTGCGG+TGG 0.634786 4_4:+57976376 Msa0690290:three_prime_UTR
ATGCTGCTGAACCTGCTCAG+TGG 0.654432 4_4:+57975408 Msa0690290:CDS
GATCCAAAGCCAGATGAAGG+TGG 0.658186 4_4:-57974990 None:intergenic
CAGCGAGATTGACAAAATCA+CGG 0.671324 4_4:-57976403 None:intergenic
GAAACTTCGTGTTAAAACCG+GGG 0.688408 4_4:+57975616 Msa0690290:CDS
GAACTGAAATCTGAGGAAAG+GGG 0.705778 4_4:+57975032 Msa0690290:CDS
ACTTCGTGTTAAAACCGGGG+AGG 0.735326 4_4:+57975619 Msa0690290:CDS
CTGACGGCGATACTATGCAG+CGG 0.754112 4_4:+57975159 Msa0690290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTATTGTCATTA+TGG - chr4_4:57976457-57976476 None:intergenic 15.0%
!! GACAATAAGAAAAAAAATAA+AGG + chr4_4:57976461-57976480 Msa0690290:three_prime_UTR 15.0%
!!! TTTTTTTCTTATTGTCATTA+TGG - chr4_4:57976457-57976476 None:intergenic 15.0%
!! GACAATAAGAAAAAAAATAA+AGG + chr4_4:57976461-57976480 Msa0690290:three_prime_UTR 15.0%
!!! TCATTTCATTTTATGTTACA+TGG + chr4_4:57974900-57974919 Msa0690290:intron 20.0%
!! TACAAAATACTTTCATACAA+AGG - chr4_4:57976507-57976526 None:intergenic 20.0%
!!! TCATTTCATTTTATGTTACA+TGG + chr4_4:57974900-57974919 Msa0690290:intron 20.0%
!! TACAAAATACTTTCATACAA+AGG - chr4_4:57976507-57976526 None:intergenic 20.0%
!! AAAAGTTATGGACTAAAACA+TGG - chr4_4:57974660-57974679 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG + chr4_4:57976015-57976034 Msa0690290:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG + chr4_4:57976160-57976179 Msa0690290:CDS 25.0%
! AACTTACCAAAATAACTTAG+AGG - chr4_4:57976199-57976218 None:intergenic 25.0%
!! AAAAGTTATGGACTAAAACA+TGG - chr4_4:57974660-57974679 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG + chr4_4:57976015-57976034 Msa0690290:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG + chr4_4:57976160-57976179 Msa0690290:CDS 25.0%
! AACTTACCAAAATAACTTAG+AGG - chr4_4:57976199-57976218 None:intergenic 25.0%
CAGAACAAAGTAACTCATTA+AGG + chr4_4:57974462-57974481 Msa0690290:five_prime_UTR 30.0%
TTCAATTTCAAAACCCATCA+AGG + chr4_4:57974553-57974572 Msa0690290:five_prime_UTR 30.0%
! TTCTTCCTTAGTCATGATTT+TGG + chr4_4:57974590-57974609 Msa0690290:exon 30.0%
ACATACCAAAATCATGACTA+AGG - chr4_4:57974598-57974617 None:intergenic 30.0%
ACAACTTACAAGAAGAAAAG+AGG - chr4_4:57974622-57974641 None:intergenic 30.0%
!!! TCCATAACTTTTTCATGAGT+CGG + chr4_4:57974668-57974687 Msa0690290:intron 30.0%
ATGAGTCTACTTGAGAATAT+TGG + chr4_4:57974809-57974828 Msa0690290:intron 30.0%
!! CAAATGCTACTTTCATGAAA+GGG - chr4_4:57975322-57975341 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG - chr4_4:57975323-57975342 None:intergenic 30.0%
CTATTATTGAAGCAATGGAA+GGG + chr4_4:57975357-57975376 Msa0690290:CDS 30.0%
TATTATTGAAGCAATGGAAG+GGG + chr4_4:57975358-57975377 Msa0690290:CDS 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG - chr4_4:57975702-57975721 None:intergenic 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG - chr4_4:57975719-57975738 None:intergenic 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG + chr4_4:57975719-57975738 Msa0690290:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG + chr4_4:57975787-57975806 Msa0690290:CDS 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG - chr4_4:57975854-57975873 None:intergenic 30.0%
GAACAAGACTCTAATCATAA+TGG + chr4_4:57975941-57975960 Msa0690290:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG + chr4_4:57975955-57975974 Msa0690290:CDS 30.0%
AAACTCATTCGAGAGATTTA+GGG + chr4_4:57976045-57976064 Msa0690290:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG + chr4_4:57976097-57976116 Msa0690290:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG + chr4_4:57976166-57976185 Msa0690290:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG + chr4_4:57976190-57976209 Msa0690290:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG + chr4_4:57976259-57976278 Msa0690290:CDS 30.0%
CAGAACAAAGTAACTCATTA+AGG + chr4_4:57974462-57974481 Msa0690290:five_prime_UTR 30.0%
TTCAATTTCAAAACCCATCA+AGG + chr4_4:57974553-57974572 Msa0690290:five_prime_UTR 30.0%
! TTCTTCCTTAGTCATGATTT+TGG + chr4_4:57974590-57974609 Msa0690290:exon 30.0%
ACATACCAAAATCATGACTA+AGG - chr4_4:57974598-57974617 None:intergenic 30.0%
ACAACTTACAAGAAGAAAAG+AGG - chr4_4:57974622-57974641 None:intergenic 30.0%
!!! TCCATAACTTTTTCATGAGT+CGG + chr4_4:57974668-57974687 Msa0690290:intron 30.0%
ATGAGTCTACTTGAGAATAT+TGG + chr4_4:57974809-57974828 Msa0690290:intron 30.0%
!! CAAATGCTACTTTCATGAAA+GGG - chr4_4:57975322-57975341 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG - chr4_4:57975323-57975342 None:intergenic 30.0%
CTATTATTGAAGCAATGGAA+GGG + chr4_4:57975357-57975376 Msa0690290:CDS 30.0%
TATTATTGAAGCAATGGAAG+GGG + chr4_4:57975358-57975377 Msa0690290:CDS 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG - chr4_4:57975702-57975721 None:intergenic 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG - chr4_4:57975719-57975738 None:intergenic 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG + chr4_4:57975719-57975738 Msa0690290:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG + chr4_4:57975787-57975806 Msa0690290:CDS 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG - chr4_4:57975854-57975873 None:intergenic 30.0%
GAACAAGACTCTAATCATAA+TGG + chr4_4:57975941-57975960 Msa0690290:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG + chr4_4:57975955-57975974 Msa0690290:CDS 30.0%
AAACTCATTCGAGAGATTTA+GGG + chr4_4:57976045-57976064 Msa0690290:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG + chr4_4:57976097-57976116 Msa0690290:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG + chr4_4:57976166-57976185 Msa0690290:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG + chr4_4:57976190-57976209 Msa0690290:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG + chr4_4:57976259-57976278 Msa0690290:CDS 30.0%
! AGAGTGTGAGTTTGAACTTT+TGG - chr4_4:57974419-57974438 None:intergenic 35.0%
TTACTTTGTTCTGTTGTTGG+TGG - chr4_4:57974455-57974474 None:intergenic 35.0%
! GAGTTACTTTGTTCTGTTGT+TGG - chr4_4:57974458-57974477 None:intergenic 35.0%
!!! GCAAGCTTTGGAATTTTCTT+TGG + chr4_4:57974512-57974531 Msa0690290:five_prime_UTR 35.0%
CAAGTATGCAATTCCTTGAT+GGG - chr4_4:57974569-57974588 None:intergenic 35.0%
ACAAGTATGCAATTCCTTGA+TGG - chr4_4:57974570-57974589 None:intergenic 35.0%
! ACCGACTCATGAAAAAGTTA+TGG - chr4_4:57974672-57974691 None:intergenic 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG - chr4_4:57975022-57975041 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG + chr4_4:57975210-57975229 Msa0690290:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG + chr4_4:57975334-57975353 Msa0690290:CDS 35.0%
TCAGGCTATTATTGAAGCAA+TGG + chr4_4:57975352-57975371 Msa0690290:CDS 35.0%
GCTATTATTGAAGCAATGGA+AGG + chr4_4:57975356-57975375 Msa0690290:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG - chr4_4:57975569-57975588 None:intergenic 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG - chr4_4:57975584-57975603 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG - chr4_4:57975607-57975626 None:intergenic 35.0%
GAGAAACTTCGTGTTAAAAC+CGG + chr4_4:57975614-57975633 Msa0690290:CDS 35.0%
AGAAACTTCGTGTTAAAACC+GGG + chr4_4:57975615-57975634 Msa0690290:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG - chr4_4:57975735-57975754 None:intergenic 35.0%
TCGAATGGAATTCACTTACA+AGG + chr4_4:57975734-57975753 Msa0690290:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG + chr4_4:57975888-57975907 Msa0690290:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG + chr4_4:57975904-57975923 Msa0690290:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG + chr4_4:57976006-57976025 Msa0690290:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG - chr4_4:57976042-57976061 None:intergenic 35.0%
AATCTCTCGAATGAGTTTCT+TGG - chr4_4:57976043-57976062 None:intergenic 35.0%
GAAACTCATTCGAGAGATTT+AGG + chr4_4:57976044-57976063 Msa0690290:CDS 35.0%
CAAGAACATTATTGCATGTG+AGG + chr4_4:57976096-57976115 Msa0690290:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG + chr4_4:57976111-57976130 Msa0690290:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG + chr4_4:57976116-57976135 Msa0690290:CDS 35.0%
TTGTGAGGCATATAGAATGA+AGG + chr4_4:57976249-57976268 Msa0690290:CDS 35.0%
CCAAGCTGATATTGAAAACA+AGG - chr4_4:57976314-57976333 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG + chr4_4:57976311-57976330 Msa0690290:CDS 35.0%
AGTAAGACGATGTGATTGTT+TGG + chr4_4:57976344-57976363 Msa0690290:exon 35.0%
ACTTGAGTTTGGCAAAATTG+CGG + chr4_4:57976373-57976392 Msa0690290:three_prime_UTR 35.0%
! AGAGTGTGAGTTTGAACTTT+TGG - chr4_4:57974419-57974438 None:intergenic 35.0%
TTACTTTGTTCTGTTGTTGG+TGG - chr4_4:57974455-57974474 None:intergenic 35.0%
! GAGTTACTTTGTTCTGTTGT+TGG - chr4_4:57974458-57974477 None:intergenic 35.0%
!!! GCAAGCTTTGGAATTTTCTT+TGG + chr4_4:57974512-57974531 Msa0690290:five_prime_UTR 35.0%
CAAGTATGCAATTCCTTGAT+GGG - chr4_4:57974569-57974588 None:intergenic 35.0%
ACAAGTATGCAATTCCTTGA+TGG - chr4_4:57974570-57974589 None:intergenic 35.0%
! ACCGACTCATGAAAAAGTTA+TGG - chr4_4:57974672-57974691 None:intergenic 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG - chr4_4:57975022-57975041 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG + chr4_4:57975210-57975229 Msa0690290:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG + chr4_4:57975334-57975353 Msa0690290:CDS 35.0%
TCAGGCTATTATTGAAGCAA+TGG + chr4_4:57975352-57975371 Msa0690290:CDS 35.0%
GCTATTATTGAAGCAATGGA+AGG + chr4_4:57975356-57975375 Msa0690290:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG - chr4_4:57975569-57975588 None:intergenic 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG - chr4_4:57975584-57975603 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG - chr4_4:57975607-57975626 None:intergenic 35.0%
GAGAAACTTCGTGTTAAAAC+CGG + chr4_4:57975614-57975633 Msa0690290:CDS 35.0%
AGAAACTTCGTGTTAAAACC+GGG + chr4_4:57975615-57975634 Msa0690290:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG - chr4_4:57975735-57975754 None:intergenic 35.0%
TCGAATGGAATTCACTTACA+AGG + chr4_4:57975734-57975753 Msa0690290:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG + chr4_4:57975888-57975907 Msa0690290:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG + chr4_4:57975904-57975923 Msa0690290:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG + chr4_4:57976006-57976025 Msa0690290:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG - chr4_4:57976042-57976061 None:intergenic 35.0%
AATCTCTCGAATGAGTTTCT+TGG - chr4_4:57976043-57976062 None:intergenic 35.0%
GAAACTCATTCGAGAGATTT+AGG + chr4_4:57976044-57976063 Msa0690290:CDS 35.0%
CAAGAACATTATTGCATGTG+AGG + chr4_4:57976096-57976115 Msa0690290:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG + chr4_4:57976111-57976130 Msa0690290:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG + chr4_4:57976116-57976135 Msa0690290:CDS 35.0%
TTGTGAGGCATATAGAATGA+AGG + chr4_4:57976249-57976268 Msa0690290:CDS 35.0%
CCAAGCTGATATTGAAAACA+AGG - chr4_4:57976314-57976333 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG + chr4_4:57976311-57976330 Msa0690290:CDS 35.0%
AGTAAGACGATGTGATTGTT+TGG + chr4_4:57976344-57976363 Msa0690290:exon 35.0%
ACTTGAGTTTGGCAAAATTG+CGG + chr4_4:57976373-57976392 Msa0690290:three_prime_UTR 35.0%
CATCACTCTTCAAATCCAAG+AGG + chr4_4:57974490-57974509 Msa0690290:five_prime_UTR 40.0%
AAATTCCAAAGCTTGCCTCT+TGG - chr4_4:57974508-57974527 None:intergenic 40.0%
TTCTGCTTTGCTTTGCTTGT+TGG + chr4_4:57974779-57974798 Msa0690290:intron 40.0%
GTACAGCTATGTTGCAAGAA+GGG + chr4_4:57974922-57974941 Msa0690290:intron 40.0%
CATCATGGAGAAGAGCTTTA+AGG - chr4_4:57974970-57974989 None:intergenic 40.0%
GATTTCAGTTCCTTAAGCCA+AGG - chr4_4:57975023-57975042 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG + chr4_4:57975025-57975044 Msa0690290:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG + chr4_4:57975030-57975049 Msa0690290:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG + chr4_4:57975031-57975050 Msa0690290:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG + chr4_4:57975032-57975051 Msa0690290:CDS 40.0%
TTGCACAAGTGAGCAACAAA+TGG - chr4_4:57975070-57975089 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG - chr4_4:57975094-57975113 None:intergenic 40.0%
TATCCTATCAGCAAGTGCTT+CGG - chr4_4:57975201-57975220 None:intergenic 40.0%
TGCTGACAACAGGATTGAAT+TGG - chr4_4:57975574-57975593 None:intergenic 40.0%
CAATCCTGTTGTCAGCAAAT+TGG + chr4_4:57975577-57975596 Msa0690290:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG + chr4_4:57975616-57975635 Msa0690290:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG - chr4_4:57975667-57975686 None:intergenic 40.0%
! AATTGCATACCCTTTTAGCC+TGG + chr4_4:57975666-57975685 Msa0690290:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG + chr4_4:57975667-57975686 Msa0690290:CDS 40.0%
TTCATCATCCCAGGCTAAAA+GGG - chr4_4:57975678-57975697 None:intergenic 40.0%
TTTCATCATCCCAGGCTAAA+AGG - chr4_4:57975679-57975698 None:intergenic 40.0%
ATATGCTTCGGTTTACTGGA+TGG - chr4_4:57975763-57975782 None:intergenic 40.0%
CCAGTAAACCGAAGCATATT+CGG + chr4_4:57975764-57975783 Msa0690290:CDS 40.0%
GCAAATTGCCGAATATGCTT+CGG - chr4_4:57975775-57975794 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG - chr4_4:57975825-57975844 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG + chr4_4:57975832-57975851 Msa0690290:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG + chr4_4:57975910-57975929 Msa0690290:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG - chr4_4:57975923-57975942 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG + chr4_4:57976064-57976083 Msa0690290:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG + chr4_4:57976069-57976088 Msa0690290:CDS 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG + chr4_4:57976129-57976148 Msa0690290:CDS 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG + chr4_4:57976209-57976228 Msa0690290:CDS 40.0%
AAGAGGTTCATGCAGAGTTA+TGG + chr4_4:57976226-57976245 Msa0690290:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG + chr4_4:57976252-57976271 Msa0690290:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG + chr4_4:57976287-57976306 Msa0690290:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG + chr4_4:57976320-57976339 Msa0690290:CDS 40.0%
CAGCGAGATTGACAAAATCA+CGG - chr4_4:57976406-57976425 None:intergenic 40.0%
! ATGGCTTATGATTTTGCGCA+TGG - chr4_4:57976438-57976457 None:intergenic 40.0%
CATCACTCTTCAAATCCAAG+AGG + chr4_4:57974490-57974509 Msa0690290:five_prime_UTR 40.0%
AAATTCCAAAGCTTGCCTCT+TGG - chr4_4:57974508-57974527 None:intergenic 40.0%
TTCTGCTTTGCTTTGCTTGT+TGG + chr4_4:57974779-57974798 Msa0690290:intron 40.0%
GTACAGCTATGTTGCAAGAA+GGG + chr4_4:57974922-57974941 Msa0690290:intron 40.0%
CATCATGGAGAAGAGCTTTA+AGG - chr4_4:57974970-57974989 None:intergenic 40.0%
GATTTCAGTTCCTTAAGCCA+AGG - chr4_4:57975023-57975042 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG + chr4_4:57975025-57975044 Msa0690290:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG + chr4_4:57975030-57975049 Msa0690290:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG + chr4_4:57975031-57975050 Msa0690290:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG + chr4_4:57975032-57975051 Msa0690290:CDS 40.0%
TTGCACAAGTGAGCAACAAA+TGG - chr4_4:57975070-57975089 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG - chr4_4:57975094-57975113 None:intergenic 40.0%
TATCCTATCAGCAAGTGCTT+CGG - chr4_4:57975201-57975220 None:intergenic 40.0%
TGCTGACAACAGGATTGAAT+TGG - chr4_4:57975574-57975593 None:intergenic 40.0%
CAATCCTGTTGTCAGCAAAT+TGG + chr4_4:57975577-57975596 Msa0690290:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG + chr4_4:57975616-57975635 Msa0690290:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG - chr4_4:57975667-57975686 None:intergenic 40.0%
! AATTGCATACCCTTTTAGCC+TGG + chr4_4:57975666-57975685 Msa0690290:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG + chr4_4:57975667-57975686 Msa0690290:CDS 40.0%
TTCATCATCCCAGGCTAAAA+GGG - chr4_4:57975678-57975697 None:intergenic 40.0%
TTTCATCATCCCAGGCTAAA+AGG - chr4_4:57975679-57975698 None:intergenic 40.0%
ATATGCTTCGGTTTACTGGA+TGG - chr4_4:57975763-57975782 None:intergenic 40.0%
CCAGTAAACCGAAGCATATT+CGG + chr4_4:57975764-57975783 Msa0690290:CDS 40.0%
GCAAATTGCCGAATATGCTT+CGG - chr4_4:57975775-57975794 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG - chr4_4:57975825-57975844 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG + chr4_4:57975832-57975851 Msa0690290:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG + chr4_4:57975910-57975929 Msa0690290:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG - chr4_4:57975923-57975942 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG + chr4_4:57976064-57976083 Msa0690290:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG + chr4_4:57976069-57976088 Msa0690290:CDS 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG + chr4_4:57976129-57976148 Msa0690290:CDS 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG + chr4_4:57976209-57976228 Msa0690290:CDS 40.0%
AAGAGGTTCATGCAGAGTTA+TGG + chr4_4:57976226-57976245 Msa0690290:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG + chr4_4:57976252-57976271 Msa0690290:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG + chr4_4:57976287-57976306 Msa0690290:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG + chr4_4:57976320-57976339 Msa0690290:CDS 40.0%
CAGCGAGATTGACAAAATCA+CGG - chr4_4:57976406-57976425 None:intergenic 40.0%
! ATGGCTTATGATTTTGCGCA+TGG - chr4_4:57976438-57976457 None:intergenic 40.0%
CAAATCCAAGAGGCAAGCTT+TGG + chr4_4:57974500-57974519 Msa0690290:five_prime_UTR 45.0%
GGTACAGCTATGTTGCAAGA+AGG + chr4_4:57974921-57974940 Msa0690290:intron 45.0%
! TTACCGAAGCACTTGCTGAT+AGG + chr4_4:57975195-57975214 Msa0690290:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG + chr4_4:57975215-57975234 Msa0690290:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG - chr4_4:57975236-57975255 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG - chr4_4:57975244-57975263 None:intergenic 45.0%
AGAAGTGAAATCCACTGAGC+AGG - chr4_4:57975422-57975441 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG - chr4_4:57975445-57975464 None:intergenic 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG + chr4_4:57975446-57975465 Msa0690290:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG - chr4_4:57975472-57975491 None:intergenic 45.0%
CCTCCACATTTGAGAATCAC+CGG + chr4_4:57975470-57975489 Msa0690290:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG - chr4_4:57975492-57975511 None:intergenic 45.0%
CAATCAGTCTATGAGCTACC+TGG - chr4_4:57975523-57975542 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG - chr4_4:57975687-57975706 None:intergenic 45.0%
CCGAATATGCTTCGGTTTAC+TGG - chr4_4:57975767-57975786 None:intergenic 45.0%
! GGTGATGATGAACTTGAGTC+AGG - chr4_4:57975833-57975852 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG + chr4_4:57975960-57975979 Msa0690290:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG - chr4_4:57975982-57976001 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG + chr4_4:57976048-57976067 Msa0690290:CDS 45.0%
GGCATGAAAAGCTGGATAAG+TGG + chr4_4:57976137-57976156 Msa0690290:CDS 45.0%
CATGCAGAGTTATGGTTGTG+AGG + chr4_4:57976234-57976253 Msa0690290:CDS 45.0%
ATGGCTGTGTGCTAATATGC+TGG + chr4_4:57976278-57976297 Msa0690290:CDS 45.0%
TTTGGACGAGCACTTGAGTT+TGG + chr4_4:57976362-57976381 Msa0690290:three_prime_UTR 45.0%
TGAGTTTGGCAAAATTGCGG+TGG + chr4_4:57976376-57976395 Msa0690290:three_prime_UTR 45.0%
CAAATCCAAGAGGCAAGCTT+TGG + chr4_4:57974500-57974519 Msa0690290:five_prime_UTR 45.0%
GGTACAGCTATGTTGCAAGA+AGG + chr4_4:57974921-57974940 Msa0690290:intron 45.0%
! TTACCGAAGCACTTGCTGAT+AGG + chr4_4:57975195-57975214 Msa0690290:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG + chr4_4:57975215-57975234 Msa0690290:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG - chr4_4:57975236-57975255 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG - chr4_4:57975244-57975263 None:intergenic 45.0%
AGAAGTGAAATCCACTGAGC+AGG - chr4_4:57975422-57975441 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG - chr4_4:57975445-57975464 None:intergenic 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG + chr4_4:57975446-57975465 Msa0690290:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG - chr4_4:57975472-57975491 None:intergenic 45.0%
CCTCCACATTTGAGAATCAC+CGG + chr4_4:57975470-57975489 Msa0690290:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG - chr4_4:57975492-57975511 None:intergenic 45.0%
CAATCAGTCTATGAGCTACC+TGG - chr4_4:57975523-57975542 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG - chr4_4:57975687-57975706 None:intergenic 45.0%
CCGAATATGCTTCGGTTTAC+TGG - chr4_4:57975767-57975786 None:intergenic 45.0%
! GGTGATGATGAACTTGAGTC+AGG - chr4_4:57975833-57975852 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG + chr4_4:57975960-57975979 Msa0690290:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG - chr4_4:57975982-57976001 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG + chr4_4:57976048-57976067 Msa0690290:CDS 45.0%
GGCATGAAAAGCTGGATAAG+TGG + chr4_4:57976137-57976156 Msa0690290:CDS 45.0%
CATGCAGAGTTATGGTTGTG+AGG + chr4_4:57976234-57976253 Msa0690290:CDS 45.0%
ATGGCTGTGTGCTAATATGC+TGG + chr4_4:57976278-57976297 Msa0690290:CDS 45.0%
TTTGGACGAGCACTTGAGTT+TGG + chr4_4:57976362-57976381 Msa0690290:three_prime_UTR 45.0%
TGAGTTTGGCAAAATTGCGG+TGG + chr4_4:57976376-57976395 Msa0690290:three_prime_UTR 45.0%
TCCATGATGCCACCTTCATC+TGG + chr4_4:57974981-57975000 Msa0690290:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG + chr4_4:57974987-57975006 Msa0690290:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG - chr4_4:57974993-57975012 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG - chr4_4:57974996-57975015 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG + chr4_4:57975010-57975029 Msa0690290:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG - chr4_4:57975017-57975036 None:intergenic 50.0%
!! CAACTCTACTCGAGTGTCGA+TGG + chr4_4:57975253-57975272 Msa0690290:CDS 50.0%
CCGGTGATTCTCAAATGTGG+AGG - chr4_4:57975473-57975492 None:intergenic 50.0%
ACGCCGGTGATTCTCAAATG+TGG - chr4_4:57975476-57975495 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG + chr4_4:57975490-57975509 Msa0690290:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG + chr4_4:57975502-57975521 Msa0690290:CDS 50.0%
ACTTCGTGTTAAAACCGGGG+AGG + chr4_4:57975619-57975638 Msa0690290:CDS 50.0%
ACTAATTGCTAGTGCCTCCC+CGG - chr4_4:57975636-57975655 None:intergenic 50.0%
TCCATGATGCCACCTTCATC+TGG + chr4_4:57974981-57975000 Msa0690290:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG + chr4_4:57974987-57975006 Msa0690290:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG - chr4_4:57974993-57975012 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG - chr4_4:57974996-57975015 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG + chr4_4:57975010-57975029 Msa0690290:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG - chr4_4:57975017-57975036 None:intergenic 50.0%
!! CAACTCTACTCGAGTGTCGA+TGG + chr4_4:57975253-57975272 Msa0690290:CDS 50.0%
CCGGTGATTCTCAAATGTGG+AGG - chr4_4:57975473-57975492 None:intergenic 50.0%
ACGCCGGTGATTCTCAAATG+TGG - chr4_4:57975476-57975495 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG + chr4_4:57975490-57975509 Msa0690290:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG + chr4_4:57975502-57975521 Msa0690290:CDS 50.0%
ACTTCGTGTTAAAACCGGGG+AGG + chr4_4:57975619-57975638 Msa0690290:CDS 50.0%
ACTAATTGCTAGTGCCTCCC+CGG - chr4_4:57975636-57975655 None:intergenic 50.0%
GCCAGATGAAGGTGGCATCA+TGG - chr4_4:57974985-57975004 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG + chr4_4:57975003-57975022 Msa0690290:CDS 55.0%
TCCATGCTTGCTTCCCCTGA+CGG + chr4_4:57975143-57975162 Msa0690290:CDS 55.0%
CTGCATAGTATCGCCGTCAG+GGG - chr4_4:57975159-57975178 None:intergenic 55.0%
GCTGCATAGTATCGCCGTCA+GGG - chr4_4:57975160-57975179 None:intergenic 55.0%
CTGACGGCGATACTATGCAG+CGG + chr4_4:57975159-57975178 Msa0690290:CDS 55.0%
ATGCTGCTGAACCTGCTCAG+TGG + chr4_4:57975408-57975427 Msa0690290:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG - chr4_4:57975464-57975483 None:intergenic 55.0%
GCCAGATGAAGGTGGCATCA+TGG - chr4_4:57974985-57975004 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG + chr4_4:57975003-57975022 Msa0690290:CDS 55.0%
TCCATGCTTGCTTCCCCTGA+CGG + chr4_4:57975143-57975162 Msa0690290:CDS 55.0%
CTGCATAGTATCGCCGTCAG+GGG - chr4_4:57975159-57975178 None:intergenic 55.0%
GCTGCATAGTATCGCCGTCA+GGG - chr4_4:57975160-57975179 None:intergenic 55.0%
CTGACGGCGATACTATGCAG+CGG + chr4_4:57975159-57975178 Msa0690290:CDS 55.0%
ATGCTGCTGAACCTGCTCAG+TGG + chr4_4:57975408-57975427 Msa0690290:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG - chr4_4:57975464-57975483 None:intergenic 55.0%
CGCTGCATAGTATCGCCGTC+AGG - chr4_4:57975161-57975180 None:intergenic 60.0%
CGCTGCATAGTATCGCCGTC+AGG - chr4_4:57975161-57975180 None:intergenic 60.0%
GCCGTCAGGGGAAGCAAGCA+TGG - chr4_4:57975147-57975166 None:intergenic 65.0%
GCCGTCAGGGGAAGCAAGCA+TGG - chr4_4:57975147-57975166 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 57974408 57976611 57974408 ID=Msa0690290;Name=Msa0690290
chr4_4 mRNA 57974408 57976611 57974408 ID=Msa0690290-mRNA-1;Parent=Msa0690290;Name=Msa0690290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=195|0|0.5|1|0|0.5|2|263|475
chr4_4 exon 57974408 57974611 57974408 ID=Msa0690290-mRNA-1:exon:15479;Parent=Msa0690290-mRNA-1
chr4_4 exon 57974930 57976611 57974930 ID=Msa0690290-mRNA-1:exon:15480;Parent=Msa0690290-mRNA-1
chr4_4 five_prime_UTR 57974408 57974602 57974408 ID=Msa0690290-mRNA-1:five_prime_utr;Parent=Msa0690290-mRNA-1
chr4_4 CDS 57974603 57974611 57974603 ID=Msa0690290-mRNA-1:cds;Parent=Msa0690290-mRNA-1
chr4_4 CDS 57974930 57976348 57974930 ID=Msa0690290-mRNA-1:cds;Parent=Msa0690290-mRNA-1
chr4_4 three_prime_UTR 57976349 57976611 57976349 ID=Msa0690290-mRNA-1:three_prime_utr;Parent=Msa0690290-mRNA-1
Gene Sequence

>Msa0690290

ATGATTTTGATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCTGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAACCATGTAGCTGCTTGTAATCTTGAGCATGCAAATGCAACACTTGAGCAAATTTCCATGCTTGCTTCCCCTGACGGCGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCACTCAACTCTACTCGAGTGTCGATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATTATTGAAGCAATGGAAGGGGAGAGAATGATTCACATAATTGATCTCAATGCTGCTGAACCTGCTCAGTGGATTTCACTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCCCTCCACATTTGAGAATCACCGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCATATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGTTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGGTCCCAAGAAACTCATTCGAGAGATTTAGGGTGGAGAAACTTCATTTTGGTGAGGAAATCAAGAACATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGAGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGCTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA
Protein sequence

>Msa0690290

MILMLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANATLEQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNAAEPAQWISLLQVLSAHPDGPPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSIFGNLLEKDLVSSYTISPDSSSSSPVSLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTVPRNSFERFRVEKLHFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVLICWQDRSLFSISAWRSRK*