AlfalfaGEDB | Msa0557350

M. sativa cultivar zhongmu-4 / Msa0557350

KEGG: -
GO: GO:0000075 GO:0000077 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0006974 GO:0008150 GO:0009314 GO:0009628 GO:0009889 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010564 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031570 GO:0033043 GO:0033554 GO:0040020 GO:0043565 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051445 GO:0051716 GO:0051726 GO:0051783 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2000241 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0557350	QDX01714.1	99.373	319	2	0	1	319	1	319	0.0	664

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0557350	sp\|F4HY61\|NAC10_ARATH	57.229	332	98	9	1	314	1	306	8.22e-119	347

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0557350	A0A6B7JET0	99.373	319	2	0	1	319	1	319	0.0	664

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TFs/TRs:

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Gene ID	Type	Classification
Msa0557350	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0557350	Msa0695240	0.897057	2.032183e-76	-8.615850e-47
Msa0557350	Msa0945200	0.812060	5.125683e-51	-8.615850e-47
Msa0557350	Msa1438250	0.800397	1.469385e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0557350	MtrunA17_Chr4g0046431	95.925	319	12	1	1	319	1	318	0.0	637
Msa0557350	MtrunA17_Chr8g0367761	98.206	223	4	0	1	223	1	223	3.60e-166	462
Msa0557350	MtrunA17_Chr2g0282871	61.231	325	92	8	1	314	1	302	1.42e-135	387
Msa0557350	MtrunA17_Chr4g0040541	65.568	273	83	6	29	295	10	277	6.47e-123	353
Msa0557350	MtrunA17_Chr2g0324961	66.078	283	84	8	27	305	10	284	1.03e-118	343
Msa0557350	MtrunA17_Chr8g0389661	70.588	204	47	5	47	241	30	229	3.16e-95	290
Msa0557350	MtrunA17_Chr5g0422431	58.081	198	80	2	44	241	36	230	3.78e-75	237
Msa0557350	MtrunA17_Chr1g0198751	47.619	252	124	4	44	295	36	279	5.01e-72	230
Msa0557350	MtrunA17_Chr7g0227071	55.978	184	74	3	47	227	36	215	8.59e-65	212
Msa0557350	MtrunA17_Chr1g0198741	52.846	123	58	0	123	245	7	129	1.07e-37	135

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0557350	AT1G28470.1	57.229	332	98	9	1	314	1	306	8.36e-120	347
Msa0557350	AT4G28500.1	70.464	237	60	5	1	236	1	228	3.94e-118	343
Msa0557350	AT4G29230.3	70.000	200	48	2	47	238	31	226	1.63e-98	297
Msa0557350	AT4G29230.1	70.000	200	48	2	47	238	31	226	5.15e-98	298
Msa0557350	AT4G29230.2	70.000	200	47	2	47	238	31	225	2.27e-97	296
Msa0557350	AT5G56620.2	65.174	201	56	4	47	236	25	222	8.27e-89	271
Msa0557350	AT5G56620.3	65.174	201	56	4	47	236	25	222	1.05e-88	271
Msa0557350	AT5G56620.1	65.174	201	56	4	47	236	25	222	1.23e-88	270
Msa0557350	AT1G25580.1	58.602	186	68	4	47	232	39	215	3.83e-69	222
Msa0557350	AT5G14490.2	49.206	189	91	4	45	232	34	218	2.03e-55	182

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Find 103 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTAAAATACCTGATCT+TGG	0.368647	4_1:+55662814	None:intergenic
AAATAAATGTGGGACTCAAG+TGG	0.568834	4_1:+55663408	None:intergenic
AAATAATTCCAATATTATCA+AGG	0.530116	4_1:-55660138	Msa0557350:CDS
AACCAAGTTTGGAATAAATT+GGG	0.365436	4_1:+55659977	None:intergenic
AACTTGGTTATGCAAGGTGA+TGG	0.581693	4_1:-55659963	Msa0557350:CDS
AAGAAGGAAGTGAGACAAGA+TGG	0.561201	4_1:-55660379	Msa0557350:CDS
AAGCATACACAACAGGAACA+AGG	0.626885	4_1:-55660427	Msa0557350:CDS
AAGGAATGAGGCAAAGGGAG+AGG	0.389085	4_1:+55663368	None:intergenic
AAGGTTCAAACTGATGAAGA+AGG	0.557893	4_1:-55660395	Msa0557350:CDS
AATAAATGTGGGACTCAAGT+GGG	0.581931	4_1:+55663409	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATATGATTGAAGTGCAAAG+AGG	+	chr4_1:55659818-55659837	None:intergenic	30.0%
CATGTCATGATCTCTTTAAT+TGG	+	chr4_1:55660100-55660119	None:intergenic	30.0%
CAATTAAAGAGATCATGACA+TGG	-	chr4_1:55660098-55660117	Msa0557350:CDS	30.0%
ATAACATCTACTGTTATTCC+TGG	-	chr4_1:55660160-55660179	Msa0557350:CDS	30.0%
TAACATTGAATTCCAAGATC+AGG	-	chr4_1:55660267-55660286	Msa0557350:CDS	30.0%
TTATCAGTAGAAATCGAACT+CGG	-	chr4_1:55660358-55660377	Msa0557350:CDS	30.0%
TTGTGTGTGAATGATTGAAT+AGG	-	chr4_1:55660466-55660485	Msa0557350:CDS	30.0%
TGTGTGTGAATGATTGAATA+GGG	-	chr4_1:55660467-55660486	Msa0557350:CDS	30.0%
TCAATAAGAGGATGAAGTTT+GGG	+	chr4_1:55660593-55660612	None:intergenic	30.0%
ATCAATAAGAGGATGAAGTT+TGG	+	chr4_1:55660594-55660613	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	55659686	55663429	55659686	ID=Msa0557350;Name=Msa0557350
chr4_1	mRNA	55659686	55663429	55659686	ID=Msa0557350-mRNA-1;Parent=Msa0557350;Name=Msa0557350-mRNA-1;_AED=0.03;_eAED=0.03;_QI=263\|1\|1\|1\|0.66\|0.5\|4\|219\|319
chr4_1	exon	55659686	55660497	55659686	ID=Msa0557350-mRNA-1:exon:16658;Parent=Msa0557350-mRNA-1
chr4_1	exon	55662438	55662627	55662438	ID=Msa0557350-mRNA-1:exon:16657;Parent=Msa0557350-mRNA-1
chr4_1	exon	55662827	55663073	55662827	ID=Msa0557350-mRNA-1:exon:16656;Parent=Msa0557350-mRNA-1
chr4_1	exon	55663237	55663429	55663237	ID=Msa0557350-mRNA-1:exon:16655;Parent=Msa0557350-mRNA-1
chr4_1	five_prime_UTR	55663237	55663429	55663237	ID=Msa0557350-mRNA-1:five_prime_utr;Parent=Msa0557350-mRNA-1
chr4_1	five_prime_UTR	55663004	55663073	55663004	ID=Msa0557350-mRNA-1:five_prime_utr;Parent=Msa0557350-mRNA-1
chr4_1	CDS	55662827	55663003	55662827	ID=Msa0557350-mRNA-1:cds;Parent=Msa0557350-mRNA-1
chr4_1	CDS	55662438	55662627	55662438	ID=Msa0557350-mRNA-1:cds;Parent=Msa0557350-mRNA-1
chr4_1	CDS	55659905	55660497	55659905	ID=Msa0557350-mRNA-1:cds;Parent=Msa0557350-mRNA-1
chr4_1	three_prime_UTR	55659686	55659904	55659686	ID=Msa0557350-mRNA-1:three_prime_utr;Parent=Msa0557350-mRNA-1

Gene Sequence

>Msa0557350

ATGACATGGTGCAATGACTCAGATGATCAAGATAGGGGAATTGAACTAATAACATCTACTGTTATTCCTGGAACTAGCAATGAAAGTACTCTTGTTACAGATGATGACACAAAACCAACCGAAGTTCGAACCCTAACTTGCCCCTCATGTGGTCATAACATTGAATTCCAAGATCAGGGTGGAATCAATGAACTGCCGGGTTTACCGGCAGGAGTGAAGTTTGATCCGAATGATCAAGAGATACTTCAGCATTTGGAAGCAAAGGTTCTGTTTGATGTGCCCAAACTTCATCCTCTTATTGATGAGTTCATACCAACACTTGAAGGAGAGAACGGAATCTGTTATACACATCCAGAAAAGCTACCAGGTGTAAAAAAAGATGGACAGATCCGGCACTTCTTTCACAGGCCATCTAAAGCATACACAACAGGAACAAGGAAAAGAAGAAAGGTTCAAACTGATGAAGAAGGAAGTGAGACAAGATGGCACAAAACAGGGAAAACTAGACCAGTTTTTATTGATGGTGTTATGAAAGGTTTCAAGAAGATACTAGTCCTCTACACCAACTATGGAAGGCAAAAGAAGCCAGAGAAAACCAATTGGGTGATGCATCAATACCACCTTGGTAGTAATGAAGAAGAGAAAGATGGTGAAATAGTTGTTTCAAAGGTGTTTTACCAGACACAGCCTCGACAATGTGGTGGAAATAATTCCAATATTATCAAGGATACTGCAAGTTATGAAAATGGACTGATGATAAATCAAGGAAATGTCCAAGATGATAATGAAGCTCCTCCTATGGATTACTACCATTCTCATTTCATAAATTATGACAATGTTGGTCATAATTTGGGAAACTCTGAATCCCAATTTATTCCAAACTTGGTTATGCAAGGTGATGGCTCTTCCTTTATTCGATTGGCAATGGATGCAAACAAAGCTGGGTTGAACAGAAATTAG

Protein sequence

>Msa0557350

MTWCNDSDDQDRGIELITSTVIPGTSNESTLVTDDDTKPTEVRTLTCPSCGHNIEFQDQGGINELPGLPAGVKFDPNDQEILQHLEAKVLFDVPKLHPLIDEFIPTLEGENGICYTHPEKLPGVKKDGQIRHFFHRPSKAYTTGTRKRRKVQTDEEGSETRWHKTGKTRPVFIDGVMKGFKKILVLYTNYGRQKKPEKTNWVMHQYHLGSNEEEKDGEIVVSKVFYQTQPRQCGGNNSNIIKDTASYENGLMINQGNVQDDNEAPPMDYYHSHFINYDNVGHNLGNSESQFIPNLVMQGDGSSFIRLAMDANKAGLNRN*