AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0560750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0560750 MtrunA17_Chr4g0050781 97.653 213 1 2 1 211 1 211 1.31e-138 386
Msa0560750 MtrunA17_Chr1g0210241 43.382 136 73 3 1 135 1 133 2.41e-31 115
Msa0560750 MtrunA17_Chr4g0035031 59.524 84 34 0 2 85 3 86 7.47e-30 109
Msa0560750 MtrunA17_Chr4g0035001 58.228 79 33 0 6 84 5 83 9.44e-29 105
Msa0560750 MtrunA17_Chr4g0035051 55.696 79 35 0 6 84 7 85 7.61e-27 99.4
Msa0560750 MtrunA17_Chr7g0266751 43.038 79 45 0 7 85 32 110 9.95e-19 79.0
Msa0560750 MtrunA17_Chr3g0086861 40.476 84 50 0 1 84 38 121 4.54e-18 81.6
Msa0560750 MtrunA17_Chr3g0119311 47.368 76 40 0 8 83 37 112 2.58e-17 75.1
Msa0560750 MtrunA17_Chr3g0116631 45.455 88 46 1 4 89 203 290 6.98e-17 77.0
Msa0560750 MtrunA17_Chr1g0168601 41.975 81 47 0 4 84 150 230 8.41e-17 76.3
Msa0560750 MtrunA17_Chr7g0263001 46.250 80 43 0 8 87 36 115 1.15e-16 73.6
Msa0560750 MtrunA17_Chr1g0198431 44.156 77 43 0 8 84 193 269 1.84e-16 75.9
Msa0560750 MtrunA17_Chr4g0061451 36.905 84 53 0 1 84 36 119 2.97e-16 75.1
Msa0560750 MtrunA17_Chr7g0219021 40.789 76 45 0 8 83 41 116 3.56e-16 74.7
Msa0560750 MtrunA17_Chr3g0121631 41.975 81 46 1 8 88 37 116 1.84e-15 70.5
Msa0560750 MtrunA17_Chr4g0017401 46.479 71 37 1 3 72 12 82 3.65e-15 72.0
Msa0560750 MtrunA17_Chr2g0327921 40.244 82 49 0 8 89 8 89 4.68e-15 67.8
Msa0560750 MtrunA17_Chr7g0276531 45.833 72 35 1 3 70 14 85 1.16e-14 70.5
Msa0560750 MtrunA17_Chr6g0481071 41.026 78 46 0 8 85 96 173 1.49e-14 68.9
Msa0560750 MtrunA17_Chr8g0346011 40.741 81 41 2 2 76 26 105 3.14e-14 69.7
Msa0560750 MtrunA17_Chr1g0181111 46.053 76 41 0 8 83 197 272 5.90e-14 68.9
Msa0560750 MtrunA17_Chr4g0058151 43.590 78 44 0 7 84 216 293 1.11e-13 68.6
Msa0560750 MtrunA17_Chr4g0058151 38.272 81 50 0 5 85 120 200 5.18e-13 66.6
Msa0560750 MtrunA17_Chr3g0088511 32.353 102 64 1 8 109 33 129 1.16e-13 65.5
Msa0560750 MtrunA17_Chr2g0290191 35.556 90 51 2 8 97 5 87 1.39e-13 68.9
Msa0560750 MtrunA17_Chr2g0290191 36.709 79 49 1 8 86 107 184 6.41e-13 67.0
Msa0560750 MtrunA17_Chr5g0447761 31.250 112 71 3 8 116 7 115 1.74e-13 68.6
Msa0560750 MtrunA17_Chr5g0447761 36.585 82 51 1 8 89 107 187 1.13e-12 66.2
Msa0560750 MtrunA17_Chr1g0186351 39.241 79 47 1 8 86 113 190 2.13e-13 68.2
Msa0560750 MtrunA17_Chr1g0186351 33.333 81 52 1 3 83 2 80 6.14e-12 63.9
Msa0560750 MtrunA17_Chr6g0449481 31.325 83 57 0 8 90 134 216 2.19e-13 68.2
Msa0560750 MtrunA17_Chr3g0101061 31.624 117 74 3 3 116 36 149 4.22e-13 67.4
Msa0560750 MtrunA17_Chr3g0101061 35.366 82 52 1 8 89 141 221 4.05e-11 61.6
Msa0560750 MtrunA17_Chr3g0144311 48.276 58 30 0 3 60 25 82 6.85e-13 65.9
Msa0560750 MtrunA17_Chr3g0120721 35.000 80 52 0 8 87 107 186 7.32e-13 66.2
Msa0560750 MtrunA17_Chr4g0042161 42.353 85 43 2 4 85 48 129 9.58e-13 63.2
Msa0560750 MtrunA17_Chr7g0267571 37.662 77 48 0 7 83 212 288 1.14e-12 65.5
Msa0560750 MtrunA17_Chr7g0268551 39.189 74 45 0 9 82 350 423 1.36e-12 65.9
Msa0560750 MtrunA17_Chr5g0426311 42.029 69 39 1 8 76 17 84 1.92e-12 64.7
Msa0560750 MtrunA17_Chr3g0135111 36.364 88 53 2 8 94 118 203 3.02e-12 64.7
Msa0560750 MtrunA17_Chr4g0065741 36.471 85 52 1 8 92 72 154 5.54e-12 61.6
Msa0560750 MtrunA17_Chr4g0031091 38.356 73 45 0 8 80 34 106 6.69e-12 60.1
Msa0560750 MtrunA17_Chr3g0080801 28.030 132 79 3 9 140 70 185 7.22e-12 63.2
Msa0560750 MtrunA17_Chr5g0419351 30.645 124 73 3 9 122 12 132 1.11e-11 60.8
Msa0560750 MtrunA17_Chr3g0110211 44.737 76 41 1 9 83 114 189 1.24e-11 62.8
Msa0560750 MtrunA17_Chr8g0371201 40.789 76 45 0 9 84 64 139 2.24e-11 61.6
Msa0560750 MtrunA17_Chr1g0177101 33.333 90 60 0 7 96 207 296 3.65e-11 61.2
Msa0560750 MtrunA17_Chr2g0281891 32.222 90 61 0 8 97 299 388 6.22e-11 61.2
Msa0560750 MtrunA17_Chr6g0484281 27.778 144 85 4 9 152 245 369 9.05e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0560750 AT3G26420.1 53.755 253 62 7 1 208 1 243 9.42e-69 211
Msa0560750 AT4G39260.3 60.976 82 32 0 4 85 3 84 1.50e-30 108
Msa0560750 AT4G39260.1 61.728 81 31 0 4 84 3 83 4.55e-29 107
Msa0560750 AT2G21660.2 60.759 79 31 0 6 84 7 85 1.13e-28 105
Msa0560750 AT1G60650.2 41.221 131 68 3 6 136 11 132 1.74e-28 108
Msa0560750 AT1G60650.1 41.221 131 68 3 6 136 11 132 1.74e-28 108
Msa0560750 AT2G21660.1 60.759 79 31 0 6 84 7 85 1.78e-28 105
Msa0560750 AT5G04280.1 38.953 172 87 4 1 159 1 167 8.87e-26 102
Msa0560750 AT4G39260.4 60.606 66 26 0 4 69 3 68 2.40e-22 87.8
Msa0560750 AT4G13850.3 46.429 84 45 0 8 91 36 119 7.54e-19 79.7
Msa0560750 AT4G13850.2 46.429 84 45 0 8 91 36 119 9.37e-19 79.7
Msa0560750 AT4G13850.4 49.351 77 39 0 8 84 36 112 1.00e-18 79.0
Msa0560750 AT4G13850.1 46.429 84 45 0 8 91 36 119 1.05e-18 79.7
Msa0560750 AT3G23830.2 43.023 86 49 0 8 93 36 121 5.24e-18 77.4
Msa0560750 AT3G23830.1 43.023 86 49 0 8 93 36 121 5.24e-18 77.4
Msa0560750 AT2G37220.1 48.052 77 40 0 8 84 205 281 1.01e-17 79.7
Msa0560750 AT2G37220.1 38.202 89 53 1 6 94 90 176 3.53e-12 64.3
Msa0560750 AT3G53460.1 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0560750 AT3G53460.3 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0560750 AT3G53460.2 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0560750 AT5G06210.1 42.500 80 46 0 8 87 35 114 6.48e-17 74.7
Msa0560750 AT5G61030.2 43.421 76 43 0 8 83 41 116 7.77e-17 77.8
Msa0560750 AT5G61030.1 43.421 76 43 0 8 83 41 116 7.77e-17 77.8
Msa0560750 AT3G53460.4 46.753 77 41 0 8 84 279 355 1.02e-16 77.8
Msa0560750 AT1G18630.1 50.000 76 38 0 8 83 37 112 1.21e-16 74.3
Msa0560750 AT1G33470.3 47.945 73 37 1 1 72 1 73 4.93e-16 74.3
Msa0560750 AT1G33470.1 47.945 73 37 1 1 72 1 73 6.09e-16 74.3
Msa0560750 AT1G33470.2 47.945 73 37 1 1 72 1 73 6.12e-16 74.3
Msa0560750 AT2G46780.1 46.667 75 39 1 2 76 17 90 1.88e-15 73.9
Msa0560750 AT2G46780.2 46.667 75 39 1 2 76 17 90 1.88e-15 73.9
Msa0560750 AT1G22910.1 46.575 73 38 1 1 72 7 79 1.99e-15 73.2
Msa0560750 AT4G24770.1 43.210 81 46 0 4 84 241 321 2.12e-15 73.9
Msa0560750 AT4G24770.1 38.272 81 50 0 5 85 148 228 1.64e-12 65.9
Msa0560750 AT4G24770.2 43.210 81 46 0 4 84 241 321 2.12e-15 73.9
Msa0560750 AT4G24770.2 38.272 81 50 0 5 85 148 228 1.64e-12 65.9
Msa0560750 AT5G04280.3 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0560750 AT5G04280.4 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0560750 AT5G04280.5 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0560750 AT5G04280.2 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0560750 AT1G22910.2 46.575 73 38 1 1 72 7 79 2.71e-15 72.4
Msa0560750 AT5G47320.1 35.922 103 64 1 8 108 32 134 3.90e-15 71.6
Msa0560750 AT5G59865.1 43.038 79 45 0 8 86 27 105 4.84e-15 68.9
Msa0560750 AT1G60000.1 41.667 84 49 0 1 84 171 254 5.22e-15 72.0
Msa0560750 AT2G21690.1 46.377 69 37 0 1 69 1 69 5.63e-15 68.9
Msa0560750 AT1G73530.1 35.514 107 61 2 8 111 78 179 5.97e-15 70.5
Msa0560750 AT1G22910.3 46.575 73 38 1 1 72 7 79 2.06e-14 71.2
Msa0560750 AT1G60650.3 36.538 104 57 3 33 136 1 95 2.17e-14 70.1
Msa0560750 AT2G37510.1 50.000 74 37 0 8 81 35 108 2.53e-14 67.8
Msa0560750 AT3G20930.1 41.558 77 45 0 8 84 283 359 3.37e-14 70.9
Msa0560750 AT1G74230.1 43.421 76 42 1 8 83 35 109 6.18e-14 69.3
Msa0560750 AT1G03457.3 41.096 73 43 0 9 81 227 299 7.38e-14 69.3
Msa0560750 AT3G13224.2 32.143 84 57 0 8 91 110 193 8.75e-14 69.3
Msa0560750 AT1G60650.4 38.776 98 51 3 39 136 1 89 1.03e-13 68.2
Msa0560750 AT3G08000.1 44.737 76 42 0 8 83 42 117 1.07e-13 66.2
Msa0560750 AT4G14300.1 37.805 82 49 1 3 84 2 81 1.08e-13 69.3
Msa0560750 AT4G14300.1 36.709 79 49 1 8 86 111 188 4.98e-12 64.7
Msa0560750 AT4G14300.2 37.805 82 49 1 3 84 2 81 1.08e-13 69.3
Msa0560750 AT4G14300.2 36.709 79 49 1 8 86 111 188 4.98e-12 64.7
Msa0560750 AT4G26650.1 32.456 114 70 2 2 115 10 116 1.21e-13 69.3
Msa0560750 AT4G26650.2 32.456 114 70 2 2 115 7 113 1.32e-13 69.3
Msa0560750 AT1G78260.1 43.478 69 38 1 8 76 18 85 1.44e-13 68.2
Msa0560750 AT3G07810.2 34.615 78 49 1 8 85 7 82 1.49e-13 69.3
Msa0560750 AT3G07810.2 37.805 82 50 1 8 89 109 189 1.25e-12 66.6
Msa0560750 AT3G07810.1 34.615 78 49 1 8 85 7 82 1.53e-13 68.9
Msa0560750 AT3G07810.1 37.805 82 50 1 8 89 109 189 1.38e-12 66.2
Msa0560750 AT3G13224.3 32.143 84 57 0 8 91 101 184 1.90e-13 68.6
Msa0560750 AT1G03457.2 41.096 73 43 0 9 81 341 413 2.25e-13 68.6
Msa0560750 AT4G13860.1 42.466 73 42 0 8 80 4 76 2.30e-13 63.5
Msa0560750 AT1G03457.1 41.096 73 43 0 9 81 332 404 2.33e-13 68.6
Msa0560750 AT1G76460.1 50.943 53 26 0 8 60 25 77 2.56e-13 67.8
Msa0560750 AT5G47620.1 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0560750 AT5G47620.2 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0560750 AT5G47620.5 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0560750 AT1G22330.1 39.189 74 44 1 3 76 13 85 7.18e-13 66.2
Msa0560750 AT1G78260.2 43.478 69 38 1 8 76 18 85 7.78e-13 66.2
Msa0560750 AT1G20880.2 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0560750 AT1G20880.3 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0560750 AT1G20880.1 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0560750 AT5G55550.5 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0560750 AT5G55550.4 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0560750 AT5G55550.9 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0560750 AT5G55550.7 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0560750 AT5G55550.1 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0560750 AT5G55550.2 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0560750 AT5G55550.3 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0560750 AT5G55550.6 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0560750 AT5G55550.11 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0560750 AT5G40490.1 36.145 83 53 0 8 90 131 213 1.32e-12 66.2
Msa0560750 AT1G58470.2 36.364 88 55 1 8 95 121 207 1.83e-12 65.9
Msa0560750 AT1G58470.1 36.364 88 55 1 8 95 121 207 1.83e-12 65.9
Msa0560750 AT5G50250.1 41.026 78 46 0 7 84 207 284 3.50e-12 64.3
Msa0560750 AT3G20930.2 39.024 82 45 1 8 84 283 364 3.76e-12 64.7
Msa0560750 AT3G54770.3 41.667 72 38 1 3 70 13 84 3.93e-12 63.9
Msa0560750 AT3G54770.1 41.667 72 38 1 3 70 13 84 4.51e-12 63.9
Msa0560750 AT5G47620.4 40.000 65 39 0 8 72 7 71 8.35e-12 63.9
Msa0560750 AT5G40490.2 37.931 87 46 2 8 90 114 196 9.15e-12 63.9
Msa0560750 AT5G53720.1 41.026 78 45 1 6 83 8 84 1.30e-11 59.3
Msa0560750 AT5G54580.1 40.449 89 47 2 4 89 53 138 1.56e-11 60.5
Msa0560750 AT4G03110.1 41.096 73 43 0 9 81 351 423 2.49e-11 62.4
Msa0560750 AT3G46020.1 34.524 84 55 0 1 84 1 84 3.09e-11 58.5
Msa0560750 AT5G55550.8 38.028 71 42 1 8 78 7 75 4.65e-11 62.0
Msa0560750 AT2G33410.1 34.177 79 51 1 8 86 111 188 5.57e-11 61.6
Msa0560750 AT2G33410.1 32.927 82 53 1 3 84 2 81 7.33e-11 61.2
Msa0560750 AT5G55550.10 38.028 71 42 1 8 78 7 75 5.67e-11 61.6

Find 130 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTGGCTATCCTGTTTC+TGG 0.103545 4_1:+60002898 Msa0560750:three_prime_UTR
TTGGCTAAATGTGGTCATTT+TGG 0.169476 4_1:+60003045 Msa0560750:three_prime_UTR
CTATCCTGTTTCTGGATCTT+TGG 0.177002 4_1:+60002906 Msa0560750:three_prime_UTR
TTCGTTCACAATGTTCTTCT+TGG 0.185957 4_1:+60002458 Msa0560750:three_prime_UTR
TATGCGTTATTGTTCTATTT+GGG 0.210106 4_1:+60003287 Msa0560750:three_prime_UTR
TTATGCGTTATTGTTCTATT+TGG 0.210748 4_1:+60003286 Msa0560750:three_prime_UTR
TCTGGACGCTCTCGTGGTTT+TGG 0.216628 4_1:+60001771 Msa0560750:CDS
CATCACATGCGGCAGTTAAA+AGG 0.266799 4_1:-60003330 None:intergenic
GGTGGTAGTCGTGGAGATTC+TGG 0.273094 4_1:+60002179 Msa0560750:CDS
AGGGATACATTTGAAAAGTT+TGG 0.280841 4_1:+60001417 Msa0560750:CDS
TTGGAAGAGTACCGTGAGTT+AGG 0.287051 4_1:-60000658 None:intergenic
TATCCTGTTTCTGGATCTTT+GGG 0.299460 4_1:+60002907 Msa0560750:three_prime_UTR
ACATGTCCAGTTAGCAATAA+AGG 0.301676 4_1:-60003083 None:intergenic
GTTCTTCTGGCGGTCGCAGC+AGG 0.318323 4_1:+60002153 Msa0560750:CDS
GTTCTTCTTGGATTGTTGTT+TGG 0.332366 4_1:+60002470 Msa0560750:three_prime_UTR
GATTCTATTTAAAGAGGAAT+TGG 0.336167 4_1:+60002764 Msa0560750:three_prime_UTR
AGTGGTGGTGGTGGTCACTA+TGG 0.339869 4_1:+60002110 Msa0560750:CDS
TTAAAAGTATAATGCCGATT+TGG 0.340839 4_1:+60002883 Msa0560750:three_prime_UTR
ACGTTCCTATGCTGGTCTTT+TGG 0.352860 4_1:+60002553 Msa0560750:three_prime_UTR
TGGGTCCCTTTATTGCTAAC+TGG 0.353192 4_1:+60003077 Msa0560750:three_prime_UTR
TTTGCAGCTTTGAAATCAAA+TGG 0.363420 4_1:+60002408 Msa0560750:intron
AGAAATGGAGATCGTTCTTC+TGG 0.378108 4_1:+60002140 Msa0560750:CDS
AAGAAGCAAAACTCTACATT+TGG 0.380905 4_1:-60002840 None:intergenic
GAACGATCTCCATTTCTATC+TGG 0.388438 4_1:-60002134 None:intergenic
AGGGAGTGCCCTAGTGGTGA+AGG 0.394081 4_1:+60002035 Msa0560750:CDS
CCATATGAACGACGTGGATC+TGG 0.395444 4_1:+60002239 Msa0560750:CDS
TATGATTCTGGCACATTATG+TGG 0.404121 4_1:+60003226 Msa0560750:three_prime_UTR
GTGGAAGAGAATGGAAGTTT+AGG 0.404584 4_1:-60003413 None:intergenic
TTCTTGGATTGTTGTTTGGA+TGG 0.418357 4_1:+60002474 Msa0560750:three_prime_UTR
CAACATCCGATAGAAAGTTA+AGG 0.426777 4_1:+60001397 Msa0560750:CDS
ACTAATTAACGTTCCTATGC+TGG 0.434677 4_1:+60002545 Msa0560750:three_prime_UTR
GTGGTTGTTGACAAATTCTC+TGG 0.436058 4_1:+60001753 Msa0560750:CDS
TACCTTGATTCTATTTAAAG+AGG 0.439783 4_1:+60002758 Msa0560750:three_prime_UTR
GAAAGTAGATATGGTGGAAG+TGG 0.439807 4_1:+60002092 Msa0560750:CDS
TATCAAAACACTAGTATCAT+TGG 0.440320 4_1:-60002498 None:intergenic
CCAGATCCACGTCGTTCATA+TGG 0.442836 4_1:-60002239 None:intergenic
TTCGCACTATTTGTCTATCA+TGG 0.443041 4_1:+60003488 Msa0560750:three_prime_UTR
CATGTCCAGTTAGCAATAAA+GGG 0.444130 4_1:-60003082 None:intergenic
TTTCCCAAAGATCCAGAAAC+AGG 0.446640 4_1:-60002910 None:intergenic
CAGAAACAGGATAGCCAAAT+CGG 0.447584 4_1:-60002897 None:intergenic
ATGGTTGAGTTCACTAGCAT+TGG 0.449938 4_1:+60003439 Msa0560750:three_prime_UTR
CTACATTTGGACCTCCATCT+TGG 0.453690 4_1:-60002827 None:intergenic
CACTATGGTCCAGATAGAAA+TGG 0.454742 4_1:+60002125 Msa0560750:CDS
TTCCTCTTTAAATAGAATCA+AGG 0.459266 4_1:-60002760 None:intergenic
CGAGATTACCGAGGTGGTCG+AGG 0.461040 4_1:+60001963 Msa0560750:CDS
TGGAAGAGTACCGTGAGTTA+GGG 0.468642 4_1:-60000657 None:intergenic
CCGTGAGTTAGGGCGGAACT+TGG 0.469870 4_1:-60000647 None:intergenic
AACGGTAGTCAAACCTGAGC+CGG 0.470968 4_1:+60003388 Msa0560750:three_prime_UTR
GGTGGTAGAGAAAGTAGATA+TGG 0.473535 4_1:+60002083 Msa0560750:CDS
CTCTATCAACAGTAATAGTT+CGG 0.474868 4_1:-60001866 None:intergenic
CAGTTTGTTGTGCTATAAAA+CGG 0.477865 4_1:+60003370 Msa0560750:three_prime_UTR
ATGAATGGGATAGATTTAGA+TGG 0.485420 4_1:+60001843 Msa0560750:CDS
AGATATGGTGGAAGTGGTGG+TGG 0.486452 4_1:+60002098 Msa0560750:CDS
GTTATTGCAGTTTAAAATGT+CGG 0.488369 4_1:+60001332 Msa0560750:intron
AACATCCGATAGAAAGTTAA+GGG 0.491433 4_1:+60001398 Msa0560750:CDS
CAAGGATCCTCTAGAGATGA+TGG 0.492928 4_1:+60001900 Msa0560750:CDS
TCTGGCGGTCGCAGCAGGGA+TGG 0.500920 4_1:+60002158 Msa0560750:CDS
TTGCAGCTTTGAAATCAAAT+GGG 0.504229 4_1:+60002409 Msa0560750:intron
TCTTCATGGGGAATATATGA+AGG 0.505584 4_1:+60003190 Msa0560750:three_prime_UTR
TATGGTGGAAGTGGTGGTGG+TGG 0.507336 4_1:+60002101 Msa0560750:CDS
GGTAGAGAAAGTAGATATGG+TGG 0.507553 4_1:+60002086 Msa0560750:CDS
AATAGTGCGAATATTTAGTA+AGG 0.509280 4_1:-60003476 None:intergenic
CATGCATGTCGAGTGCTGCT+TGG 0.509513 4_1:+60002675 Msa0560750:three_prime_UTR
CAAACCACATTTCTATGTGC+TGG 0.509981 4_1:+60003683 Msa0560750:three_prime_UTR
ATAAAAGAAGAATAAACAAA+AGG 0.510192 4_1:+60000560 Msa0560750:five_prime_UTR
AACCATGTCGTGGAAGAGAA+TGG 0.511194 4_1:-60003422 None:intergenic
ATTAAATACCTCGATGAGAA+AGG 0.513257 4_1:-60002282 None:intergenic
AAATTCTCTGGACGCTCTCG+TGG 0.522134 4_1:+60001765 Msa0560750:CDS
TGTGCCAGAATCATAGAAAA+CGG 0.524140 4_1:-60003218 None:intergenic
GATGCTATTGATGCTATGAA+TGG 0.526053 4_1:+60001828 Msa0560750:CDS
TATCGGATGTTGACCAAGCA+AGG 0.526702 4_1:-60001386 None:intergenic
ATGCGTTATTGTTCTATTTG+GGG 0.526965 4_1:+60003288 Msa0560750:three_prime_UTR
TGTATCCCTTAACTTTCTAT+CGG 0.530467 4_1:-60001403 None:intergenic
ATGCTATCAATAATTGCTGC+TGG 0.532558 4_1:+60002630 Msa0560750:three_prime_UTR
GGAAGTTTAGGACCCGGCTC+AGG 0.533784 4_1:-60003401 None:intergenic
TGCTTCAATTGTGGTAAACC+TGG 0.534286 4_1:+60002002 Msa0560750:CDS
TTCCATTCTCTTCCACGACA+TGG 0.538850 4_1:+60003420 Msa0560750:three_prime_UTR
GGGAGTGCCCTAGTGGTGAA+GGG 0.544084 4_1:+60002036 Msa0560750:CDS
TTCTTCTGGCGGTCGCAGCA+GGG 0.544328 4_1:+60002154 Msa0560750:CDS
CACGATCGCGACCACGCTCT+CGG 0.544700 4_1:-60001932 None:intergenic
TGGCAAGCTTACAGAGGCCA+AGG 0.548700 4_1:+60001437 Msa0560750:CDS
ACGGTAGTCAAACCTGAGCC+GGG 0.550013 4_1:+60003389 Msa0560750:three_prime_UTR
TATCGTGATCGTGATCGTGC+TGG 0.552506 4_1:+60002215 Msa0560750:CDS
GCAGGATTCTTGGCTAAATG+TGG 0.559112 4_1:+60003036 Msa0560750:intron
CTTCATGGGGAATATATGAA+GGG 0.560134 4_1:+60003191 Msa0560750:three_prime_UTR
GGCGGTCGCAGCAGGGATGG+TGG 0.563764 4_1:+60002161 Msa0560750:CDS
ACAAACCAAAAGACCAGCAT+AGG 0.565468 4_1:-60002558 None:intergenic
TGCGTTATTGTTCTATTTGG+GGG 0.565803 4_1:+60003289 Msa0560750:three_prime_UTR
AGCAGGGATGGTGGTAGTCG+TGG 0.574692 4_1:+60002170 Msa0560750:CDS
TTTATGCATGTCGAGTTGCA+TGG 0.575230 4_1:+60002718 Msa0560750:three_prime_UTR
AAAATGATCAAATCTAACAT+TGG 0.578255 4_1:-60003597 None:intergenic
CACTCCCTAGCAAAATGACC+AGG 0.578775 4_1:-60002020 None:intergenic
GGTGGTCGAGGATCTAATGA+TGG 0.580077 4_1:+60001975 Msa0560750:CDS
GGAGTGCCCTAGTGGTGAAG+GGG 0.582980 4_1:+60002037 Msa0560750:CDS
AGTAGATATGGTGGAAGTGG+TGG 0.583787 4_1:+60002095 Msa0560750:CDS
GCAGTTTAAAATGTCGGACG+AGG 0.587416 4_1:+60001338 Msa0560750:intron
CTTTGGGAAAGTAGTCAAGC+GGG 0.590635 4_1:+60002923 Msa0560750:three_prime_UTR
ATGCTATTGATGCTATGAAT+GGG 0.592487 4_1:+60001829 Msa0560750:CDS
TTTGCTAGGGAGTGCCCTAG+TGG 0.594989 4_1:+60002029 Msa0560750:CDS
TTTGTGGCTTTGGATGTTTG+GGG 0.601798 4_1:+60002792 Msa0560750:three_prime_UTR
GAACGACGTGGATCTGGAGG+CGG 0.604365 4_1:+60002245 Msa0560750:CDS
AACGTTAATTAGTCTAACAA+AGG 0.610574 4_1:-60002535 None:intergenic
ATATGATGAAAAGAAAGCAA+TGG 0.616878 4_1:+60001803 Msa0560750:CDS
GAGAATGGAAGTTTAGGACC+CGG 0.619545 4_1:-60003407 None:intergenic
CCAAGTTCCGCCCTAACTCA+CGG 0.631741 4_1:+60000647 Msa0560750:five_prime_UTR
GAGTGCCCTAGTGGTGAAGG+GGG 0.631815 4_1:+60002038 Msa0560750:CDS
GGTGATCGCTACCGAGAGCG+TGG 0.633883 4_1:+60001921 Msa0560750:CDS
TTCTTCTCATTGGAAGAACG+AGG 0.636743 4_1:+60002858 Msa0560750:three_prime_UTR
TCTCTAGAGGATCCTTGTTG+AGG 0.640001 4_1:-60001894 None:intergenic
TTCATGGGGAATATATGAAG+GGG 0.640366 4_1:+60003192 Msa0560750:three_prime_UTR
TATGAACGACGTGGATCTGG+AGG 0.640937 4_1:+60002242 Msa0560750:CDS
GATAGAGCTCAGCCTCAACA+AGG 0.641644 4_1:+60001882 Msa0560750:CDS
GCGATCACCATCATCTCTAG+AGG 0.642537 4_1:-60001907 None:intergenic
CATTAGATCCTCGACCACCT+CGG 0.645981 4_1:-60001971 None:intergenic
AATGGAGATCGTTCTTCTGG+CGG 0.649243 4_1:+60002143 Msa0560750:CDS
AACAAACAAAACATCACATG+CGG 0.657583 4_1:-60003341 None:intergenic
AAGAGTACCGTGAGTTAGGG+CGG 0.658136 4_1:-60000654 None:intergenic
TTGATATACCTTTCTCATCG+AGG 0.661732 4_1:+60002274 Msa0560750:exon
GATGGAGGATGCTTCAATTG+TGG 0.663732 4_1:+60001993 Msa0560750:CDS
AAAGTTTGGCAAGCTTACAG+AGG 0.664669 4_1:+60001431 Msa0560750:CDS
CCCCATGAAGAAAACAAGAG+CGG 0.666738 4_1:-60003178 None:intergenic
TCTTTGGGAAAGTAGTCAAG+CGG 0.667376 4_1:+60002922 Msa0560750:three_prime_UTR
GGTCGAGGATCTAATGATGG+AGG 0.667781 4_1:+60001978 Msa0560750:CDS
AACTCCAGCACATAGAAATG+TGG 0.671634 4_1:-60003687 None:intergenic
TCATGGGGAATATATGAAGG+GGG 0.691942 4_1:+60003193 Msa0560750:three_prime_UTR
TAGTGAACTCAACCATGTCG+TGG 0.697075 4_1:-60003432 None:intergenic
CGTGATCGAGATTACCGAGG+TGG 0.697615 4_1:+60001957 Msa0560750:CDS
ATAGAATCAAGGTAATCCCG+CGG 0.718822 4_1:-60002749 None:intergenic
GCTGGACCATATGAACGACG+TGG 0.755617 4_1:+60002233 Msa0560750:CDS
GAACGTGATCGAGATTACCG+AGG 0.756337 4_1:+60001954 Msa0560750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTGTATTTTTTATTTTATTT+TGG + chr4_1:60002305-60002324 Msa0560750:intron 10.0%
!! ATAAAAGAAGAATAAACAAA+AGG + chr4_1:60000560-60000579 Msa0560750:five_prime_UTR 15.0%
!! TATGATAGAAAATAACTAAA+GGG - chr4_1:60001154-60001173 None:intergenic 15.0%
!! TTATGATAGAAAATAACTAA+AGG - chr4_1:60001155-60001174 None:intergenic 15.0%
!! TTACTTGATTTACTAATAAA+AGG - chr4_1:60001614-60001633 None:intergenic 15.0%
!! CAATGTAATATGATAAAAAA+TGG + chr4_1:60001651-60001670 Msa0560750:intron 15.0%
!!! TTTCATTTTATTTTGTGAAA+AGG + chr4_1:60001731-60001750 Msa0560750:intron 15.0%
!! TGAAAAAAATACAATTTGAA+AGG - chr4_1:60003562-60003581 None:intergenic 15.0%
!! TTAAAAATCGGATTTAAATC+AGG - chr4_1:60000748-60000767 None:intergenic 20.0%
!! ATAACATAAGAATTAGTACA+CGG - chr4_1:60001317-60001336 None:intergenic 20.0%
!!! CTTTTGTTCATTTTTATTTG+AGG + chr4_1:60001564-60001583 Msa0560750:intron 20.0%
!! TCTGAACATTATATTTGATT+TGG + chr4_1:60001684-60001703 Msa0560750:intron 20.0%
!!! ATTATATTTGATTTGGAGAA+TGG + chr4_1:60001691-60001710 Msa0560750:intron 20.0%
!!! TTTATTTTATTTTGGACGAA+TGG + chr4_1:60002313-60002332 Msa0560750:intron 20.0%
!! AAAATGATCAAATCTAACAT+TGG - chr4_1:60003600-60003619 None:intergenic 20.0%
! AAGAAAAAAGTGGAAGAATT+TGG - chr4_1:60000680-60000699 None:intergenic 25.0%
! ATGAAGAATGAAGAAAAAAG+TGG - chr4_1:60000690-60000709 None:intergenic 25.0%
!! GTTTTGTATAATTCGTGTTT+GGG + chr4_1:60000795-60000814 Msa0560750:intron 25.0%
! GTTGAAAATTCAAACTTATG+AGG - chr4_1:60001105-60001124 None:intergenic 25.0%
!! TTCTATTAGCTTATTGTTTG+AGG + chr4_1:60001225-60001244 Msa0560750:intron 25.0%
!! GTTATTGCAGTTTAAAATGT+CGG + chr4_1:60001332-60001351 Msa0560750:intron 25.0%
!!! AAAGATAAAGTTTGTTGTCT+AGG - chr4_1:60001548-60001567 None:intergenic 25.0%
!!! CATTTTATTTTGTGAAAAGG+TGG + chr4_1:60001734-60001753 Msa0560750:intron 25.0%
! ATATGATGAAAAGAAAGCAA+TGG + chr4_1:60001803-60001822 Msa0560750:CDS 25.0%
! TATCAAAACACTAGTATCAT+TGG - chr4_1:60002501-60002520 None:intergenic 25.0%
!! AACGTTAATTAGTCTAACAA+AGG - chr4_1:60002538-60002557 None:intergenic 25.0%
! TACCTTGATTCTATTTAAAG+AGG + chr4_1:60002758-60002777 Msa0560750:three_prime_UTR 25.0%
! TTCCTCTTTAAATAGAATCA+AGG - chr4_1:60002763-60002782 None:intergenic 25.0%
! GATTCTATTTAAAGAGGAAT+TGG + chr4_1:60002764-60002783 Msa0560750:three_prime_UTR 25.0%
!! TTAAAAGTATAATGCCGATT+TGG + chr4_1:60002883-60002902 Msa0560750:three_prime_UTR 25.0%
!! TATTGATGTTATTGAGCTTT+TGG + chr4_1:60003014-60003033 Msa0560750:intron 25.0%
! ACAATAACGCATAAAAACAA+TGG - chr4_1:60003280-60003299 None:intergenic 25.0%
! TTATGCGTTATTGTTCTATT+TGG + chr4_1:60003286-60003305 Msa0560750:three_prime_UTR 25.0%
! TATGCGTTATTGTTCTATTT+GGG + chr4_1:60003287-60003306 Msa0560750:three_prime_UTR 25.0%
! AATAGTGCGAATATTTAGTA+AGG - chr4_1:60003479-60003498 None:intergenic 25.0%
ATCGGATTTAAATCAGGAAA+AGG - chr4_1:60000742-60000761 None:intergenic 30.0%
GAATTGTCACGGTTAAAAAT+CGG - chr4_1:60000760-60000779 None:intergenic 30.0%
!! GGTTTTGTATAATTCGTGTT+TGG + chr4_1:60000794-60000813 Msa0560750:intron 30.0%
!! TCACTGGTTTACTTGAATTT+AGG + chr4_1:60000823-60000842 Msa0560750:intron 30.0%
! GTTTACTTGAATTTAGGATC+AGG + chr4_1:60000829-60000848 Msa0560750:intron 30.0%
GTAATTGTTGTCATCATTGT+AGG + chr4_1:60001029-60001048 Msa0560750:intron 30.0%
GTTATTCCTTTGTAGAGATT+TGG + chr4_1:60001069-60001088 Msa0560750:intron 30.0%
AACATTAGCACAAAGTTTGA+TGG + chr4_1:60001122-60001141 Msa0560750:intron 30.0%
GTTATGTGTGTTAATTACCT+TGG + chr4_1:60001247-60001266 Msa0560750:intron 30.0%
AACATCCGATAGAAAGTTAA+GGG + chr4_1:60001398-60001417 Msa0560750:CDS 30.0%
TGTATCCCTTAACTTTCTAT+CGG - chr4_1:60001406-60001425 None:intergenic 30.0%
AGGGATACATTTGAAAAGTT+TGG + chr4_1:60001417-60001436 Msa0560750:CDS 30.0%
! ATTTGAGATATTGATCACCT+TGG - chr4_1:60001457-60001476 None:intergenic 30.0%
ATGCTATTGATGCTATGAAT+GGG + chr4_1:60001829-60001848 Msa0560750:CDS 30.0%
ATGAATGGGATAGATTTAGA+TGG + chr4_1:60001843-60001862 Msa0560750:CDS 30.0%
CTCTATCAACAGTAATAGTT+CGG - chr4_1:60001869-60001888 None:intergenic 30.0%
ATTAAATACCTCGATGAGAA+AGG - chr4_1:60002285-60002304 None:intergenic 30.0%
!! TTTGCAGCTTTGAAATCAAA+TGG + chr4_1:60002408-60002427 Msa0560750:intron 30.0%
!! TTGCAGCTTTGAAATCAAAT+GGG + chr4_1:60002409-60002428 Msa0560750:intron 30.0%
!!! GTGTTTTGATATGCAATACT+TGG + chr4_1:60002510-60002529 Msa0560750:three_prime_UTR 30.0%
AAGAAGCAAAACTCTACATT+TGG - chr4_1:60002843-60002862 None:intergenic 30.0%
!! GTCATTTTGGATTCATCAAT+GGG + chr4_1:60003058-60003077 Msa0560750:three_prime_UTR 30.0%
ATGCGTTATTGTTCTATTTG+GGG + chr4_1:60003288-60003307 Msa0560750:three_prime_UTR 30.0%
AACAAACAAAACATCACATG+CGG - chr4_1:60003344-60003363 None:intergenic 30.0%
!! CAGTTTGTTGTGCTATAAAA+CGG + chr4_1:60003370-60003389 Msa0560750:three_prime_UTR 30.0%
TCTTCTGTTTGTGTGTATTG+AGG + chr4_1:60000864-60000883 Msa0560750:intron 35.0%
TTTGTGTGTATTGAGGATTG+AGG + chr4_1:60000871-60000890 Msa0560750:intron 35.0%
TATGTGATAGCTGTGAAGTT+TGG + chr4_1:60000971-60000990 Msa0560750:intron 35.0%
TCCTTTGTAGAGATTTGGAA+AGG + chr4_1:60001074-60001093 Msa0560750:intron 35.0%
ACCTTTCCAAATCTCTACAA+AGG - chr4_1:60001078-60001097 None:intergenic 35.0%
GTGTTGAATTAATCAGTCCA+AGG - chr4_1:60001267-60001286 None:intergenic 35.0%
TTAATTCAACACCGTGTAGT+TGG + chr4_1:60001275-60001294 Msa0560750:intron 35.0%
!! GATGAGTATCGTTGTTTTGT+TGG + chr4_1:60001360-60001379 Msa0560750:CDS 35.0%
CAACATCCGATAGAAAGTTA+AGG + chr4_1:60001397-60001416 Msa0560750:CDS 35.0%
TCATGTTTGTACCTAGAAGT+TGG + chr4_1:60001498-60001517 Msa0560750:intron 35.0%
TACAGCATAAACCAACTTCT+AGG - chr4_1:60001512-60001531 None:intergenic 35.0%
GATGCTATTGATGCTATGAA+TGG + chr4_1:60001828-60001847 Msa0560750:CDS 35.0%
!! TAAACCTGGTCATTTTGCTA+GGG + chr4_1:60002016-60002035 Msa0560750:CDS 35.0%
TTGATATACCTTTCTCATCG+AGG + chr4_1:60002274-60002293 Msa0560750:exon 35.0%
TTCGTTCACAATGTTCTTCT+TGG + chr4_1:60002458-60002477 Msa0560750:three_prime_UTR 35.0%
! GTTCTTCTTGGATTGTTGTT+TGG + chr4_1:60002470-60002489 Msa0560750:three_prime_UTR 35.0%
!! TTCTTGGATTGTTGTTTGGA+TGG + chr4_1:60002474-60002493 Msa0560750:three_prime_UTR 35.0%
ACTAATTAACGTTCCTATGC+TGG + chr4_1:60002545-60002564 Msa0560750:three_prime_UTR 35.0%
ATGCTATCAATAATTGCTGC+TGG + chr4_1:60002630-60002649 Msa0560750:three_prime_UTR 35.0%
! AGAGGAATTGGAATGTTTTG+TGG + chr4_1:60002776-60002795 Msa0560750:three_prime_UTR 35.0%
! ATTGGAATGTTTTGTGGCTT+TGG + chr4_1:60002782-60002801 Msa0560750:three_prime_UTR 35.0%
!!! TTTTGTGGCTTTGGATGTTT+GGG + chr4_1:60002791-60002810 Msa0560750:three_prime_UTR 35.0%
!!! GGTTTTTTCATGTACCAAGA+TGG + chr4_1:60002813-60002832 Msa0560750:three_prime_UTR 35.0%
! TTTTTCATGTACCAAGATGG+AGG + chr4_1:60002816-60002835 Msa0560750:three_prime_UTR 35.0%
! AGAGTTTTGCTTCTTCTCAT+TGG + chr4_1:60002848-60002867 Msa0560750:three_prime_UTR 35.0%
!! TATCCTGTTTCTGGATCTTT+GGG + chr4_1:60002907-60002926 Msa0560750:three_prime_UTR 35.0%
! TTGGCTAAATGTGGTCATTT+TGG + chr4_1:60003045-60003064 Msa0560750:three_prime_UTR 35.0%
!! GGTCATTTTGGATTCATCAA+TGG + chr4_1:60003057-60003076 Msa0560750:three_prime_UTR 35.0%
CATGTCCAGTTAGCAATAAA+GGG - chr4_1:60003085-60003104 None:intergenic 35.0%
ACATGTCCAGTTAGCAATAA+AGG - chr4_1:60003086-60003105 None:intergenic 35.0%
TCTTCATGGGGAATATATGA+AGG + chr4_1:60003190-60003209 Msa0560750:three_prime_UTR 35.0%
CTTCATGGGGAATATATGAA+GGG + chr4_1:60003191-60003210 Msa0560750:three_prime_UTR 35.0%
TTCATGGGGAATATATGAAG+GGG + chr4_1:60003192-60003211 Msa0560750:three_prime_UTR 35.0%
TGTGCCAGAATCATAGAAAA+CGG - chr4_1:60003221-60003240 None:intergenic 35.0%
TATGATTCTGGCACATTATG+TGG + chr4_1:60003226-60003245 Msa0560750:three_prime_UTR 35.0%
TGCGTTATTGTTCTATTTGG+GGG + chr4_1:60003289-60003308 Msa0560750:three_prime_UTR 35.0%
! CGTTTTATAGCACAACAAAC+TGG - chr4_1:60003372-60003391 None:intergenic 35.0%
TTCGCACTATTTGTCTATCA+TGG + chr4_1:60003488-60003507 Msa0560750:three_prime_UTR 35.0%
! AAATTTTCCTGCAAAAGCTC+TGG - chr4_1:60003719-60003738 None:intergenic 35.0%
ACAATTCGCTCAGGAACATT+GGG + chr4_1:60000773-60000792 Msa0560750:intron 40.0%
! CATACTATTGTGCTGCTGTT+TGG + chr4_1:60001186-60001205 Msa0560750:intron 40.0%
AATTAGTCGGTCCAACTACA+CGG - chr4_1:60001289-60001308 None:intergenic 40.0%
! GTACACGGTGTTGAATTAGT+CGG - chr4_1:60001302-60001321 None:intergenic 40.0%
!! GAGTATCGTTGTTTTGTTGG+TGG + chr4_1:60001363-60001382 Msa0560750:CDS 40.0%
AAAGTTTGGCAAGCTTACAG+AGG + chr4_1:60001431-60001450 Msa0560750:CDS 40.0%
GTGGTTGTTGACAAATTCTC+TGG + chr4_1:60001753-60001772 Msa0560750:CDS 40.0%
TGCTTCAATTGTGGTAAACC+TGG + chr4_1:60002002-60002021 Msa0560750:CDS 40.0%
!! GTAAACCTGGTCATTTTGCT+AGG + chr4_1:60002015-60002034 Msa0560750:CDS 40.0%
GGTGGTAGAGAAAGTAGATA+TGG + chr4_1:60002083-60002102 Msa0560750:CDS 40.0%
GGTAGAGAAAGTAGATATGG+TGG + chr4_1:60002086-60002105 Msa0560750:CDS 40.0%
! GAAAGTAGATATGGTGGAAG+TGG + chr4_1:60002092-60002111 Msa0560750:CDS 40.0%
CACTATGGTCCAGATAGAAA+TGG + chr4_1:60002125-60002144 Msa0560750:CDS 40.0%
GAACGATCTCCATTTCTATC+TGG - chr4_1:60002137-60002156 None:intergenic 40.0%
AGAAATGGAGATCGTTCTTC+TGG + chr4_1:60002140-60002159 Msa0560750:CDS 40.0%
ACAAACCAAAAGACCAGCAT+AGG - chr4_1:60002561-60002580 None:intergenic 40.0%
! TTTATGCATGTCGAGTTGCA+TGG + chr4_1:60002718-60002737 Msa0560750:three_prime_UTR 40.0%
ATAGAATCAAGGTAATCCCG+CGG - chr4_1:60002752-60002771 None:intergenic 40.0%
!!! GTTTTGTGGCTTTGGATGTT+TGG + chr4_1:60002790-60002809 Msa0560750:three_prime_UTR 40.0%
!! TTTGTGGCTTTGGATGTTTG+GGG + chr4_1:60002792-60002811 Msa0560750:three_prime_UTR 40.0%
TTCTTCTCATTGGAAGAACG+AGG + chr4_1:60002858-60002877 Msa0560750:three_prime_UTR 40.0%
CAGAAACAGGATAGCCAAAT+CGG - chr4_1:60002900-60002919 None:intergenic 40.0%
!! CTATCCTGTTTCTGGATCTT+TGG + chr4_1:60002906-60002925 Msa0560750:three_prime_UTR 40.0%
TTTCCCAAAGATCCAGAAAC+AGG - chr4_1:60002913-60002932 None:intergenic 40.0%
! TCTTTGGGAAAGTAGTCAAG+CGG + chr4_1:60002922-60002941 Msa0560750:three_prime_UTR 40.0%
ACTTCACCATCACTAGAGTT+AGG + chr4_1:60002976-60002995 Msa0560750:intron 40.0%
CTGAAACCTAACTCTAGTGA+TGG - chr4_1:60002985-60003004 None:intergenic 40.0%
! GATGTTATTGAGCTTTTGGC+AGG + chr4_1:60003018-60003037 Msa0560750:intron 40.0%
! CAAATCTTTTCAGCTGCAAG+AGG + chr4_1:60003127-60003146 Msa0560750:three_prime_UTR 40.0%
!!! TACCGCTCTTGTTTTCTTCA+TGG + chr4_1:60003176-60003195 Msa0560750:three_prime_UTR 40.0%
!!! ACCGCTCTTGTTTTCTTCAT+GGG + chr4_1:60003177-60003196 Msa0560750:three_prime_UTR 40.0%
TCATGGGGAATATATGAAGG+GGG + chr4_1:60003193-60003212 Msa0560750:three_prime_UTR 40.0%
! GGTTCCGTTTTCTATGATTC+TGG + chr4_1:60003214-60003233 Msa0560750:three_prime_UTR 40.0%
GTGGAAGAGAATGGAAGTTT+AGG - chr4_1:60003416-60003435 None:intergenic 40.0%
ATGGTTGAGTTCACTAGCAT+TGG + chr4_1:60003439-60003458 Msa0560750:three_prime_UTR 40.0%
CAAACCACATTTCTATGTGC+TGG + chr4_1:60003683-60003702 Msa0560750:three_prime_UTR 40.0%
AACTCCAGCACATAGAAATG+TGG - chr4_1:60003690-60003709 None:intergenic 40.0%
!! TGGAAGAGTACCGTGAGTTA+GGG - chr4_1:60000660-60000679 None:intergenic 45.0%
!! TTGGAAGAGTACCGTGAGTT+AGG - chr4_1:60000661-60000680 None:intergenic 45.0%
TTAACCGTGACAATTCGCTC+AGG + chr4_1:60000764-60000783 Msa0560750:intron 45.0%
TGTTCCTGAGCGAATTGTCA+CGG - chr4_1:60000771-60000790 None:intergenic 45.0%
GACAATTCGCTCAGGAACAT+TGG + chr4_1:60000772-60000791 Msa0560750:intron 45.0%
TCGTGTTTGGGAAACATCAC+TGG + chr4_1:60000807-60000826 Msa0560750:intron 45.0%
! GCAGTTTAAAATGTCGGACG+AGG + chr4_1:60001338-60001357 Msa0560750:intron 45.0%
TATCGGATGTTGACCAAGCA+AGG - chr4_1:60001389-60001408 None:intergenic 45.0%
! TCTCTAGAGGATCCTTGTTG+AGG - chr4_1:60001897-60001916 None:intergenic 45.0%
! CAAGGATCCTCTAGAGATGA+TGG + chr4_1:60001900-60001919 Msa0560750:CDS 45.0%
GATGGAGGATGCTTCAATTG+TGG + chr4_1:60001993-60002012 Msa0560750:CDS 45.0%
! AGTAGATATGGTGGAAGTGG+TGG + chr4_1:60002095-60002114 Msa0560750:CDS 45.0%
AATGGAGATCGTTCTTCTGG+CGG + chr4_1:60002143-60002162 Msa0560750:CDS 45.0%
!!! ACGTTCCTATGCTGGTCTTT+TGG + chr4_1:60002553-60002572 Msa0560750:three_prime_UTR 45.0%
! GTTGCATGGCATATTTTCCG+CGG + chr4_1:60002732-60002751 Msa0560750:three_prime_UTR 45.0%
! TTGCATGGCATATTTTCCGC+GGG + chr4_1:60002733-60002752 Msa0560750:three_prime_UTR 45.0%
CTACATTTGGACCTCCATCT+TGG - chr4_1:60002830-60002849 None:intergenic 45.0%
! CGATTTGGCTATCCTGTTTC+TGG + chr4_1:60002898-60002917 Msa0560750:three_prime_UTR 45.0%
! CTTTGGGAAAGTAGTCAAGC+GGG + chr4_1:60002923-60002942 Msa0560750:three_prime_UTR 45.0%
! TGAGCTTTTGGCAGGATTCT+TGG + chr4_1:60003026-60003045 Msa0560750:intron 45.0%
GCAGGATTCTTGGCTAAATG+TGG + chr4_1:60003036-60003055 Msa0560750:intron 45.0%
TGGGTCCCTTTATTGCTAAC+TGG + chr4_1:60003077-60003096 Msa0560750:three_prime_UTR 45.0%
CCCCATGAAGAAAACAAGAG+CGG - chr4_1:60003181-60003200 None:intergenic 45.0%
!!! CCGCTCTTGTTTTCTTCATG+GGG + chr4_1:60003178-60003197 Msa0560750:three_prime_UTR 45.0%
CATCACATGCGGCAGTTAAA+AGG - chr4_1:60003333-60003352 None:intergenic 45.0%
! GAGAATGGAAGTTTAGGACC+CGG - chr4_1:60003410-60003429 None:intergenic 45.0%
TTCCATTCTCTTCCACGACA+TGG + chr4_1:60003420-60003439 Msa0560750:three_prime_UTR 45.0%
AACCATGTCGTGGAAGAGAA+TGG - chr4_1:60003425-60003444 None:intergenic 45.0%
TAGTGAACTCAACCATGTCG+TGG - chr4_1:60003435-60003454 None:intergenic 45.0%
!!! TTTTTTTTTTTTATTGATTT+AGG + chr4_1:60000912-60000931 Msa0560750:intron 5.0%
!! AAGAGTACCGTGAGTTAGGG+CGG - chr4_1:60000657-60000676 None:intergenic 50.0%
!!! GTTTTGTTGGTGGCCTTGCT+TGG + chr4_1:60001373-60001392 Msa0560750:CDS 50.0%
AAATTCTCTGGACGCTCTCG+TGG + chr4_1:60001765-60001784 Msa0560750:CDS 50.0%
GATAGAGCTCAGCCTCAACA+AGG + chr4_1:60001882-60001901 Msa0560750:CDS 50.0%
GCGATCACCATCATCTCTAG+AGG - chr4_1:60001910-60001929 None:intergenic 50.0%
GAACGTGATCGAGATTACCG+AGG + chr4_1:60001954-60001973 Msa0560750:CDS 50.0%
CATTAGATCCTCGACCACCT+CGG - chr4_1:60001974-60001993 None:intergenic 50.0%
GGTGGTCGAGGATCTAATGA+TGG + chr4_1:60001975-60001994 Msa0560750:CDS 50.0%
GGTCGAGGATCTAATGATGG+AGG + chr4_1:60001978-60001997 Msa0560750:CDS 50.0%
CACTCCCTAGCAAAATGACC+AGG - chr4_1:60002023-60002042 None:intergenic 50.0%
! AGATATGGTGGAAGTGGTGG+TGG + chr4_1:60002098-60002117 Msa0560750:CDS 50.0%
TATCGTGATCGTGATCGTGC+TGG + chr4_1:60002215-60002234 Msa0560750:CDS 50.0%
CCAGATCCACGTCGTTCATA+TGG - chr4_1:60002242-60002261 None:intergenic 50.0%
CCATATGAACGACGTGGATC+TGG + chr4_1:60002239-60002258 Msa0560750:CDS 50.0%
TATGAACGACGTGGATCTGG+AGG + chr4_1:60002242-60002261 Msa0560750:CDS 50.0%
AACGGTAGTCAAACCTGAGC+CGG + chr4_1:60003388-60003407 Msa0560750:three_prime_UTR 50.0%
CGCTCTCTCTCTCTCTCTCT+AGG - chr4_1:60000614-60000633 None:intergenic 55.0%
CCAAGTTCCGCCCTAACTCA+CGG + chr4_1:60000647-60000666 Msa0560750:five_prime_UTR 55.0%
TGGCAAGCTTACAGAGGCCA+AGG + chr4_1:60001437-60001456 Msa0560750:CDS 55.0%
! TCTGGACGCTCTCGTGGTTT+TGG + chr4_1:60001771-60001790 Msa0560750:CDS 55.0%
CGTGATCGAGATTACCGAGG+TGG + chr4_1:60001957-60001976 Msa0560750:CDS 55.0%
!! TTTGCTAGGGAGTGCCCTAG+TGG + chr4_1:60002029-60002048 Msa0560750:CDS 55.0%
CTCTTCCCCCTTCACCACTA+GGG - chr4_1:60002046-60002065 None:intergenic 55.0%
CTAGTGGTGAAGGGGGAAGA+GGG + chr4_1:60002045-60002064 Msa0560750:CDS 55.0%
TAGTGGTGAAGGGGGAAGAG+GGG + chr4_1:60002046-60002065 Msa0560750:CDS 55.0%
!! TATGGTGGAAGTGGTGGTGG+TGG + chr4_1:60002101-60002120 Msa0560750:CDS 55.0%
!! AGTGGTGGTGGTGGTCACTA+TGG + chr4_1:60002110-60002129 Msa0560750:CDS 55.0%
GGTGGTAGTCGTGGAGATTC+TGG + chr4_1:60002179-60002198 Msa0560750:CDS 55.0%
GCTGGACCATATGAACGACG+TGG + chr4_1:60002233-60002252 Msa0560750:CDS 55.0%
! CATGCATGTCGAGTGCTGCT+TGG + chr4_1:60002675-60002694 Msa0560750:three_prime_UTR 55.0%
ACGGTAGTCAAACCTGAGCC+GGG + chr4_1:60003389-60003408 Msa0560750:three_prime_UTR 55.0%
! TCGTGTGCCAGAGCTTTTGC+AGG + chr4_1:60003709-60003728 Msa0560750:three_prime_UTR 55.0%
! AGAGAGAGAGAGCGCAGAGC+AGG + chr4_1:60000620-60000639 Msa0560750:five_prime_UTR 60.0%
CCGTGAGTTAGGGCGGAACT+TGG - chr4_1:60000650-60000669 None:intergenic 60.0%
CGAGATTACCGAGGTGGTCG+AGG + chr4_1:60001963-60001982 Msa0560750:CDS 60.0%
!! AGGGAGTGCCCTAGTGGTGA+AGG + chr4_1:60002035-60002054 Msa0560750:CDS 60.0%
!! GGGAGTGCCCTAGTGGTGAA+GGG + chr4_1:60002036-60002055 Msa0560750:CDS 60.0%
! GGAGTGCCCTAGTGGTGAAG+GGG + chr4_1:60002037-60002056 Msa0560750:CDS 60.0%
GAGTGCCCTAGTGGTGAAGG+GGG + chr4_1:60002038-60002057 Msa0560750:CDS 60.0%
CCTCTTCCCCCTTCACCACT+AGG - chr4_1:60002047-60002066 None:intergenic 60.0%
CCTAGTGGTGAAGGGGGAAG+AGG + chr4_1:60002044-60002063 Msa0560750:CDS 60.0%
AGTGGTGAAGGGGGAAGAGG+GGG + chr4_1:60002047-60002066 Msa0560750:CDS 60.0%
TTCTTCTGGCGGTCGCAGCA+GGG + chr4_1:60002154-60002173 Msa0560750:CDS 60.0%
! AGCAGGGATGGTGGTAGTCG+TGG + chr4_1:60002170-60002189 Msa0560750:CDS 60.0%
GAACGACGTGGATCTGGAGG+CGG + chr4_1:60002245-60002264 Msa0560750:CDS 60.0%
! GGAAGTTTAGGACCCGGCTC+AGG - chr4_1:60003404-60003423 None:intergenic 60.0%
GGTGATCGCTACCGAGAGCG+TGG + chr4_1:60001921-60001940 Msa0560750:CDS 65.0%
CACGATCGCGACCACGCTCT+CGG - chr4_1:60001935-60001954 None:intergenic 65.0%
GTGAAGGGGGAAGAGGGGGA+GGG + chr4_1:60002051-60002070 Msa0560750:CDS 65.0%
TGAAGGGGGAAGAGGGGGAG+GGG + chr4_1:60002052-60002071 Msa0560750:CDS 65.0%
AGAGGGGGAGGGGGAAGGTA+TGG + chr4_1:60002062-60002081 Msa0560750:CDS 65.0%
GTTCTTCTGGCGGTCGCAGC+AGG + chr4_1:60002153-60002172 Msa0560750:CDS 65.0%
GGTGAAGGGGGAAGAGGGGG+AGG + chr4_1:60002050-60002069 Msa0560750:CDS 70.0%
GAAGGGGGAAGAGGGGGAGG+GGG + chr4_1:60002053-60002072 Msa0560750:CDS 70.0%
! GGGGGAGGGGGAAGGTATGG+TGG + chr4_1:60002065-60002084 Msa0560750:CDS 70.0%
TCTGGCGGTCGCAGCAGGGA+TGG + chr4_1:60002158-60002177 Msa0560750:CDS 70.0%
GGGGAAGAGGGGGAGGGGGA+AGG + chr4_1:60002057-60002076 Msa0560750:CDS 75.0%
GGCGGTCGCAGCAGGGATGG+TGG + chr4_1:60002161-60002180 Msa0560750:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 60000560 60003750 60000560 ID=Msa0560750;Name=Msa0560750
chr4_1 mRNA 60000560 60003750 60000560 ID=Msa0560750-mRNA-1;Parent=Msa0560750;Name=Msa0560750-mRNA-1;_AED=0.09;_eAED=0.09;_QI=115|1|1|1|0.5|0.4|5|1259|211
chr4_1 exon 60000560 60000668 60000560 ID=Msa0560750-mRNA-1:exon:18689;Parent=Msa0560750-mRNA-1
chr4_1 exon 60001342 60001458 60001342 ID=Msa0560750-mRNA-1:exon:18690;Parent=Msa0560750-mRNA-1
chr4_1 exon 60001753 60002295 60001753 ID=Msa0560750-mRNA-1:exon:18691;Parent=Msa0560750-mRNA-1
chr4_1 exon 60002415 60002944 60002415 ID=Msa0560750-mRNA-1:exon:18692;Parent=Msa0560750-mRNA-1
chr4_1 exon 60003040 60003750 60003040 ID=Msa0560750-mRNA-1:exon:18693;Parent=Msa0560750-mRNA-1
chr4_1 five_prime_UTR 60000560 60000668 60000560 ID=Msa0560750-mRNA-1:five_prime_utr;Parent=Msa0560750-mRNA-1
chr4_1 five_prime_UTR 60001342 60001347 60001342 ID=Msa0560750-mRNA-1:five_prime_utr;Parent=Msa0560750-mRNA-1
chr4_1 CDS 60001348 60001458 60001348 ID=Msa0560750-mRNA-1:cds;Parent=Msa0560750-mRNA-1
chr4_1 CDS 60001753 60002277 60001753 ID=Msa0560750-mRNA-1:cds;Parent=Msa0560750-mRNA-1
chr4_1 three_prime_UTR 60002278 60002295 60002278 ID=Msa0560750-mRNA-1:three_prime_utr;Parent=Msa0560750-mRNA-1
chr4_1 three_prime_UTR 60002415 60002944 60002415 ID=Msa0560750-mRNA-1:three_prime_utr;Parent=Msa0560750-mRNA-1
chr4_1 three_prime_UTR 60003040 60003750 60003040 ID=Msa0560750-mRNA-1:three_prime_utr;Parent=Msa0560750-mRNA-1
Gene Sequence

>Msa0560750

ATGTCGGACGAGGATGAGTATCGTTGTTTTGTTGGTGGCCTTGCTTGGTCAACATCCGATAGAAAGTTAAGGGATACATTTGAAAAGTTTGGCAAGCTTACAGAGGCCAAGGTGGTTGTTGACAAATTCTCTGGACGCTCTCGTGGTTTTGGATTTGTCACATATGATGAAAAGAAAGCAATGGAAGATGCTATTGATGCTATGAATGGGATAGATTTAGATGGCCGAACTATTACTGTTGATAGAGCTCAGCCTCAACAAGGATCCTCTAGAGATGATGGTGATCGCTACCGAGAGCGTGGTCGCGATCGTGAACGTGATCGAGATTACCGAGGTGGTCGAGGATCTAATGATGGAGGATGCTTCAATTGTGGTAAACCTGGTCATTTTGCTAGGGAGTGCCCTAGTGGTGAAGGGGGAAGAGGGGGAGGGGGAAGGTATGGTGGTAGAGAAAGTAGATATGGTGGAAGTGGTGGTGGTGGTCACTATGGTCCAGATAGAAATGGAGATCGTTCTTCTGGCGGTCGCAGCAGGGATGGTGGTAGTCGTGGAGATTCTGGAAGTGATCGATACTATCGTGATCGTGATCGTGCTGGACCATATGAACGACGTGGATCTGGAGGCGGCTATCGTTGA
Protein sequence

>Msa0560750

MSDEDEYRCFVGGLAWSTSDRKLRDTFEKFGKLTEAKVVVDKFSGRSRGFGFVTYDEKKAMEDAIDAMNGIDLDGRTITVDRAQPQQGSSRDDGDRYRERGRDRERDRDYRGGRGSNDGGCFNCGKPGHFARECPSGEGGRGGGGRYGGRESRYGGSGGGGHYGPDRNGDRSSGGRSRDGGSRGDSGSDRYYRDRDRAGPYERRGSGGGYR*