AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0697940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0697940 MtrunA17_Chr4g0050781 97.653 213 1 2 1 211 1 211 1.31e-138 386
Msa0697940 MtrunA17_Chr1g0210241 43.382 136 73 3 1 135 1 133 2.41e-31 115
Msa0697940 MtrunA17_Chr4g0035031 59.524 84 34 0 2 85 3 86 7.47e-30 109
Msa0697940 MtrunA17_Chr4g0035001 58.228 79 33 0 6 84 5 83 9.44e-29 105
Msa0697940 MtrunA17_Chr4g0035051 55.696 79 35 0 6 84 7 85 7.61e-27 99.4
Msa0697940 MtrunA17_Chr7g0266751 43.038 79 45 0 7 85 32 110 9.95e-19 79.0
Msa0697940 MtrunA17_Chr3g0086861 40.476 84 50 0 1 84 38 121 4.54e-18 81.6
Msa0697940 MtrunA17_Chr3g0119311 47.368 76 40 0 8 83 37 112 2.58e-17 75.1
Msa0697940 MtrunA17_Chr3g0116631 45.455 88 46 1 4 89 203 290 6.98e-17 77.0
Msa0697940 MtrunA17_Chr1g0168601 41.975 81 47 0 4 84 150 230 8.41e-17 76.3
Msa0697940 MtrunA17_Chr7g0263001 46.250 80 43 0 8 87 36 115 1.15e-16 73.6
Msa0697940 MtrunA17_Chr1g0198431 44.156 77 43 0 8 84 193 269 1.84e-16 75.9
Msa0697940 MtrunA17_Chr4g0061451 36.905 84 53 0 1 84 36 119 2.97e-16 75.1
Msa0697940 MtrunA17_Chr7g0219021 40.789 76 45 0 8 83 41 116 3.56e-16 74.7
Msa0697940 MtrunA17_Chr3g0121631 41.975 81 46 1 8 88 37 116 1.84e-15 70.5
Msa0697940 MtrunA17_Chr4g0017401 46.479 71 37 1 3 72 12 82 3.65e-15 72.0
Msa0697940 MtrunA17_Chr2g0327921 40.244 82 49 0 8 89 8 89 4.68e-15 67.8
Msa0697940 MtrunA17_Chr7g0276531 45.833 72 35 1 3 70 14 85 1.16e-14 70.5
Msa0697940 MtrunA17_Chr6g0481071 41.026 78 46 0 8 85 96 173 1.49e-14 68.9
Msa0697940 MtrunA17_Chr8g0346011 40.741 81 41 2 2 76 26 105 3.14e-14 69.7
Msa0697940 MtrunA17_Chr1g0181111 46.053 76 41 0 8 83 197 272 5.90e-14 68.9
Msa0697940 MtrunA17_Chr4g0058151 43.590 78 44 0 7 84 216 293 1.11e-13 68.6
Msa0697940 MtrunA17_Chr4g0058151 38.272 81 50 0 5 85 120 200 5.18e-13 66.6
Msa0697940 MtrunA17_Chr3g0088511 32.353 102 64 1 8 109 33 129 1.16e-13 65.5
Msa0697940 MtrunA17_Chr2g0290191 35.556 90 51 2 8 97 5 87 1.39e-13 68.9
Msa0697940 MtrunA17_Chr2g0290191 36.709 79 49 1 8 86 107 184 6.41e-13 67.0
Msa0697940 MtrunA17_Chr5g0447761 31.250 112 71 3 8 116 7 115 1.74e-13 68.6
Msa0697940 MtrunA17_Chr5g0447761 36.585 82 51 1 8 89 107 187 1.13e-12 66.2
Msa0697940 MtrunA17_Chr1g0186351 39.241 79 47 1 8 86 113 190 2.13e-13 68.2
Msa0697940 MtrunA17_Chr1g0186351 33.333 81 52 1 3 83 2 80 6.14e-12 63.9
Msa0697940 MtrunA17_Chr6g0449481 31.325 83 57 0 8 90 134 216 2.19e-13 68.2
Msa0697940 MtrunA17_Chr3g0101061 31.624 117 74 3 3 116 36 149 4.22e-13 67.4
Msa0697940 MtrunA17_Chr3g0101061 35.366 82 52 1 8 89 141 221 4.05e-11 61.6
Msa0697940 MtrunA17_Chr3g0144311 48.276 58 30 0 3 60 25 82 6.85e-13 65.9
Msa0697940 MtrunA17_Chr3g0120721 35.000 80 52 0 8 87 107 186 7.32e-13 66.2
Msa0697940 MtrunA17_Chr4g0042161 42.353 85 43 2 4 85 48 129 9.58e-13 63.2
Msa0697940 MtrunA17_Chr7g0267571 37.662 77 48 0 7 83 212 288 1.14e-12 65.5
Msa0697940 MtrunA17_Chr7g0268551 39.189 74 45 0 9 82 350 423 1.36e-12 65.9
Msa0697940 MtrunA17_Chr5g0426311 42.029 69 39 1 8 76 17 84 1.92e-12 64.7
Msa0697940 MtrunA17_Chr3g0135111 36.364 88 53 2 8 94 118 203 3.02e-12 64.7
Msa0697940 MtrunA17_Chr4g0065741 36.471 85 52 1 8 92 72 154 5.54e-12 61.6
Msa0697940 MtrunA17_Chr4g0031091 38.356 73 45 0 8 80 34 106 6.69e-12 60.1
Msa0697940 MtrunA17_Chr3g0080801 28.030 132 79 3 9 140 70 185 7.22e-12 63.2
Msa0697940 MtrunA17_Chr5g0419351 30.645 124 73 3 9 122 12 132 1.11e-11 60.8
Msa0697940 MtrunA17_Chr3g0110211 44.737 76 41 1 9 83 114 189 1.24e-11 62.8
Msa0697940 MtrunA17_Chr8g0371201 40.789 76 45 0 9 84 64 139 2.24e-11 61.6
Msa0697940 MtrunA17_Chr1g0177101 33.333 90 60 0 7 96 207 296 3.65e-11 61.2
Msa0697940 MtrunA17_Chr2g0281891 32.222 90 61 0 8 97 299 388 6.22e-11 61.2
Msa0697940 MtrunA17_Chr6g0484281 27.778 144 85 4 9 152 245 369 9.05e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0697940 AT3G26420.1 53.755 253 62 7 1 208 1 243 9.42e-69 211
Msa0697940 AT4G39260.3 60.976 82 32 0 4 85 3 84 1.50e-30 108
Msa0697940 AT4G39260.1 61.728 81 31 0 4 84 3 83 4.55e-29 107
Msa0697940 AT2G21660.2 60.759 79 31 0 6 84 7 85 1.13e-28 105
Msa0697940 AT1G60650.2 41.221 131 68 3 6 136 11 132 1.74e-28 108
Msa0697940 AT1G60650.1 41.221 131 68 3 6 136 11 132 1.74e-28 108
Msa0697940 AT2G21660.1 60.759 79 31 0 6 84 7 85 1.78e-28 105
Msa0697940 AT5G04280.1 38.953 172 87 4 1 159 1 167 8.87e-26 102
Msa0697940 AT4G39260.4 60.606 66 26 0 4 69 3 68 2.40e-22 87.8
Msa0697940 AT4G13850.3 46.429 84 45 0 8 91 36 119 7.54e-19 79.7
Msa0697940 AT4G13850.2 46.429 84 45 0 8 91 36 119 9.37e-19 79.7
Msa0697940 AT4G13850.4 49.351 77 39 0 8 84 36 112 1.00e-18 79.0
Msa0697940 AT4G13850.1 46.429 84 45 0 8 91 36 119 1.05e-18 79.7
Msa0697940 AT3G23830.2 43.023 86 49 0 8 93 36 121 5.24e-18 77.4
Msa0697940 AT3G23830.1 43.023 86 49 0 8 93 36 121 5.24e-18 77.4
Msa0697940 AT2G37220.1 48.052 77 40 0 8 84 205 281 1.01e-17 79.7
Msa0697940 AT2G37220.1 38.202 89 53 1 6 94 90 176 3.53e-12 64.3
Msa0697940 AT3G53460.1 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0697940 AT3G53460.3 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0697940 AT3G53460.2 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0697940 AT5G06210.1 42.500 80 46 0 8 87 35 114 6.48e-17 74.7
Msa0697940 AT5G61030.2 43.421 76 43 0 8 83 41 116 7.77e-17 77.8
Msa0697940 AT5G61030.1 43.421 76 43 0 8 83 41 116 7.77e-17 77.8
Msa0697940 AT3G53460.4 46.753 77 41 0 8 84 279 355 1.02e-16 77.8
Msa0697940 AT1G18630.1 50.000 76 38 0 8 83 37 112 1.21e-16 74.3
Msa0697940 AT1G33470.3 47.945 73 37 1 1 72 1 73 4.93e-16 74.3
Msa0697940 AT1G33470.1 47.945 73 37 1 1 72 1 73 6.09e-16 74.3
Msa0697940 AT1G33470.2 47.945 73 37 1 1 72 1 73 6.12e-16 74.3
Msa0697940 AT2G46780.1 46.667 75 39 1 2 76 17 90 1.88e-15 73.9
Msa0697940 AT2G46780.2 46.667 75 39 1 2 76 17 90 1.88e-15 73.9
Msa0697940 AT1G22910.1 46.575 73 38 1 1 72 7 79 1.99e-15 73.2
Msa0697940 AT4G24770.1 43.210 81 46 0 4 84 241 321 2.12e-15 73.9
Msa0697940 AT4G24770.1 38.272 81 50 0 5 85 148 228 1.64e-12 65.9
Msa0697940 AT4G24770.2 43.210 81 46 0 4 84 241 321 2.12e-15 73.9
Msa0697940 AT4G24770.2 38.272 81 50 0 5 85 148 228 1.64e-12 65.9
Msa0697940 AT5G04280.3 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0697940 AT5G04280.4 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0697940 AT5G04280.5 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0697940 AT5G04280.2 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0697940 AT1G22910.2 46.575 73 38 1 1 72 7 79 2.71e-15 72.4
Msa0697940 AT5G47320.1 35.922 103 64 1 8 108 32 134 3.90e-15 71.6
Msa0697940 AT5G59865.1 43.038 79 45 0 8 86 27 105 4.84e-15 68.9
Msa0697940 AT1G60000.1 41.667 84 49 0 1 84 171 254 5.22e-15 72.0
Msa0697940 AT2G21690.1 46.377 69 37 0 1 69 1 69 5.63e-15 68.9
Msa0697940 AT1G73530.1 35.514 107 61 2 8 111 78 179 5.97e-15 70.5
Msa0697940 AT1G22910.3 46.575 73 38 1 1 72 7 79 2.06e-14 71.2
Msa0697940 AT1G60650.3 36.538 104 57 3 33 136 1 95 2.17e-14 70.1
Msa0697940 AT2G37510.1 50.000 74 37 0 8 81 35 108 2.53e-14 67.8
Msa0697940 AT3G20930.1 41.558 77 45 0 8 84 283 359 3.37e-14 70.9
Msa0697940 AT1G74230.1 43.421 76 42 1 8 83 35 109 6.18e-14 69.3
Msa0697940 AT1G03457.3 41.096 73 43 0 9 81 227 299 7.38e-14 69.3
Msa0697940 AT3G13224.2 32.143 84 57 0 8 91 110 193 8.75e-14 69.3
Msa0697940 AT1G60650.4 38.776 98 51 3 39 136 1 89 1.03e-13 68.2
Msa0697940 AT3G08000.1 44.737 76 42 0 8 83 42 117 1.07e-13 66.2
Msa0697940 AT4G14300.1 37.805 82 49 1 3 84 2 81 1.08e-13 69.3
Msa0697940 AT4G14300.1 36.709 79 49 1 8 86 111 188 4.98e-12 64.7
Msa0697940 AT4G14300.2 37.805 82 49 1 3 84 2 81 1.08e-13 69.3
Msa0697940 AT4G14300.2 36.709 79 49 1 8 86 111 188 4.98e-12 64.7
Msa0697940 AT4G26650.1 32.456 114 70 2 2 115 10 116 1.21e-13 69.3
Msa0697940 AT4G26650.2 32.456 114 70 2 2 115 7 113 1.32e-13 69.3
Msa0697940 AT1G78260.1 43.478 69 38 1 8 76 18 85 1.44e-13 68.2
Msa0697940 AT3G07810.2 34.615 78 49 1 8 85 7 82 1.49e-13 69.3
Msa0697940 AT3G07810.2 37.805 82 50 1 8 89 109 189 1.25e-12 66.6
Msa0697940 AT3G07810.1 34.615 78 49 1 8 85 7 82 1.53e-13 68.9
Msa0697940 AT3G07810.1 37.805 82 50 1 8 89 109 189 1.38e-12 66.2
Msa0697940 AT3G13224.3 32.143 84 57 0 8 91 101 184 1.90e-13 68.6
Msa0697940 AT1G03457.2 41.096 73 43 0 9 81 341 413 2.25e-13 68.6
Msa0697940 AT4G13860.1 42.466 73 42 0 8 80 4 76 2.30e-13 63.5
Msa0697940 AT1G03457.1 41.096 73 43 0 9 81 332 404 2.33e-13 68.6
Msa0697940 AT1G76460.1 50.943 53 26 0 8 60 25 77 2.56e-13 67.8
Msa0697940 AT5G47620.1 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0697940 AT5G47620.2 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0697940 AT5G47620.5 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0697940 AT1G22330.1 39.189 74 44 1 3 76 13 85 7.18e-13 66.2
Msa0697940 AT1G78260.2 43.478 69 38 1 8 76 18 85 7.78e-13 66.2
Msa0697940 AT1G20880.2 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0697940 AT1G20880.3 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0697940 AT1G20880.1 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0697940 AT5G55550.5 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0697940 AT5G55550.4 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0697940 AT5G55550.9 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0697940 AT5G55550.7 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0697940 AT5G55550.1 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0697940 AT5G55550.2 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0697940 AT5G55550.3 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0697940 AT5G55550.6 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0697940 AT5G55550.11 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0697940 AT5G40490.1 36.145 83 53 0 8 90 131 213 1.32e-12 66.2
Msa0697940 AT1G58470.2 36.364 88 55 1 8 95 121 207 1.83e-12 65.9
Msa0697940 AT1G58470.1 36.364 88 55 1 8 95 121 207 1.83e-12 65.9
Msa0697940 AT5G50250.1 41.026 78 46 0 7 84 207 284 3.50e-12 64.3
Msa0697940 AT3G20930.2 39.024 82 45 1 8 84 283 364 3.76e-12 64.7
Msa0697940 AT3G54770.3 41.667 72 38 1 3 70 13 84 3.93e-12 63.9
Msa0697940 AT3G54770.1 41.667 72 38 1 3 70 13 84 4.51e-12 63.9
Msa0697940 AT5G47620.4 40.000 65 39 0 8 72 7 71 8.35e-12 63.9
Msa0697940 AT5G40490.2 37.931 87 46 2 8 90 114 196 9.15e-12 63.9
Msa0697940 AT5G53720.1 41.026 78 45 1 6 83 8 84 1.30e-11 59.3
Msa0697940 AT5G54580.1 40.449 89 47 2 4 89 53 138 1.56e-11 60.5
Msa0697940 AT4G03110.1 41.096 73 43 0 9 81 351 423 2.49e-11 62.4
Msa0697940 AT3G46020.1 34.524 84 55 0 1 84 1 84 3.09e-11 58.5
Msa0697940 AT5G55550.8 38.028 71 42 1 8 78 7 75 4.65e-11 62.0
Msa0697940 AT2G33410.1 34.177 79 51 1 8 86 111 188 5.57e-11 61.6
Msa0697940 AT2G33410.1 32.927 82 53 1 3 84 2 81 7.33e-11 61.2
Msa0697940 AT5G55550.10 38.028 71 42 1 8 78 7 75 5.67e-11 61.6

Find 129 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTGGCTATCCTGTTTC+TGG 0.103545 4_4:+67997976 Msa0697940:three_prime_UTR
TTGGCTAAATGTGGTCATTT+TGG 0.169476 4_4:+67998112 Msa0697940:three_prime_UTR
CTATCCTGTTTCTGGATCTT+TGG 0.177002 4_4:+67997984 Msa0697940:three_prime_UTR
TTCGTTCACAATGTTCTTCT+TGG 0.185957 4_4:+67997536 Msa0697940:three_prime_UTR
TATGCGTTATTGTTCTATTT+GGG 0.210106 4_4:+67998356 Msa0697940:three_prime_UTR
TTATGCGTTATTGTTCTATT+TGG 0.210748 4_4:+67998355 Msa0697940:three_prime_UTR
TCTGGACGCTCTCGTGGTTT+TGG 0.216628 4_4:+67996849 Msa0697940:CDS
CATCACATGCGGCAGTTAAA+AGG 0.266799 4_4:-67998399 None:intergenic
GGTGGTAGTCGTGGAGATTC+TGG 0.273094 4_4:+67997257 Msa0697940:CDS
AGGGATACATTTGAAAAGTT+TGG 0.280841 4_4:+67996495 Msa0697940:CDS
TTGGAAGAGTACCGTGAGTT+AGG 0.287051 4_4:-67995683 None:intergenic
TATCCTGTTTCTGGATCTTT+GGG 0.299460 4_4:+67997985 Msa0697940:three_prime_UTR
ACATGTCCAGTTAGCAATAA+AGG 0.301676 4_4:-67998150 None:intergenic
GTTCTTCTGGCGGTCGCAGC+AGG 0.318323 4_4:+67997231 Msa0697940:CDS
GTTCTTCTTGGATTGTTGTT+TGG 0.332366 4_4:+67997548 Msa0697940:three_prime_UTR
GATTCTATTTAAAGAGGAAT+TGG 0.336167 4_4:+67997842 Msa0697940:three_prime_UTR
AGTGGTGGTGGTGGTCACTA+TGG 0.339869 4_4:+67997188 Msa0697940:CDS
TTAAAAGTATAATGCCGATT+TGG 0.340839 4_4:+67997961 Msa0697940:three_prime_UTR
ACGTTCCTATGCTGGTCTTT+TGG 0.352860 4_4:+67997631 Msa0697940:three_prime_UTR
TGGGTCCCTTTATTGCTAAC+TGG 0.353192 4_4:+67998144 Msa0697940:three_prime_UTR
TTTGCAGCTTTGAAATCAAA+TGG 0.363420 4_4:+67997486 Msa0697940:intron
AGAAATGGAGATCGTTCTTC+TGG 0.378108 4_4:+67997218 Msa0697940:CDS
AAGAAGCAAAACTCTACATT+TGG 0.380905 4_4:-67997918 None:intergenic
GAACGATCTCCATTTCTATC+TGG 0.388438 4_4:-67997212 None:intergenic
AGGGAGTGCCCTAGTGGTGA+AGG 0.394081 4_4:+67997113 Msa0697940:CDS
CCATATGAACGACGTGGATC+TGG 0.395444 4_4:+67997317 Msa0697940:CDS
TATGATTCTGGCACATTATG+TGG 0.404121 4_4:+67998295 Msa0697940:three_prime_UTR
GTGGAAGAGAATGGAAGTTT+AGG 0.404584 4_4:-67998482 None:intergenic
TTCTTGGATTGTTGTTTGGA+TGG 0.418357 4_4:+67997552 Msa0697940:three_prime_UTR
CAACATCCGATAGAAAGTTA+AGG 0.426777 4_4:+67996475 Msa0697940:CDS
GTTCGTGTGCCAGAGCTTGC+AGG 0.428329 4_4:+67998776 Msa0697940:three_prime_UTR
ACTAATTAACGTTCCTATGC+TGG 0.434677 4_4:+67997623 Msa0697940:three_prime_UTR
GTGGTTGTTGACAAATTCTC+TGG 0.436058 4_4:+67996831 Msa0697940:CDS
TACCTTGATTCTATTTAAAG+AGG 0.439783 4_4:+67997836 Msa0697940:three_prime_UTR
GAAAGTAGATATGGTGGAAG+TGG 0.439807 4_4:+67997170 Msa0697940:CDS
TATCAAAACACTAGTATCAT+TGG 0.440320 4_4:-67997576 None:intergenic
CCAGATCCACGTCGTTCATA+TGG 0.442836 4_4:-67997317 None:intergenic
TTCGCACTATTTGTCTATCA+TGG 0.443041 4_4:+67998557 Msa0697940:three_prime_UTR
CATGTCCAGTTAGCAATAAA+GGG 0.444130 4_4:-67998149 None:intergenic
TTTCCCAAAGATCCAGAAAC+AGG 0.446640 4_4:-67997988 None:intergenic
CAGAAACAGGATAGCCAAAT+CGG 0.447584 4_4:-67997975 None:intergenic
ATGGTTGAGTTCACTAGCAT+TGG 0.449938 4_4:+67998508 Msa0697940:three_prime_UTR
CTACATTTGGACCTCCATCT+TGG 0.453690 4_4:-67997905 None:intergenic
CACTATGGTCCAGATAGAAA+TGG 0.454742 4_4:+67997203 Msa0697940:CDS
TTCCTCTTTAAATAGAATCA+AGG 0.459266 4_4:-67997838 None:intergenic
CGAGATTACCGAGGTGGTCG+AGG 0.461040 4_4:+67997041 Msa0697940:CDS
TGGAAGAGTACCGTGAGTTA+GGG 0.468642 4_4:-67995682 None:intergenic
CCGTGAGTTAGGGCGGAACT+TGG 0.469870 4_4:-67995672 None:intergenic
GGTGGTAGAGAAAGTAGATA+TGG 0.473535 4_4:+67997161 Msa0697940:CDS
CTCTATCAACAGTAATAGTT+CGG 0.474868 4_4:-67996944 None:intergenic
CAGTTTGTTGTGCTATAAAA+CGG 0.477865 4_4:+67998439 Msa0697940:three_prime_UTR
ATGAATGGGATAGATTTAGA+TGG 0.485420 4_4:+67996921 Msa0697940:CDS
AGATATGGTGGAAGTGGTGG+TGG 0.486452 4_4:+67997176 Msa0697940:CDS
AACATCCGATAGAAAGTTAA+GGG 0.491433 4_4:+67996476 Msa0697940:CDS
CAAGGATCCTCTAGAGATGA+TGG 0.492928 4_4:+67996978 Msa0697940:CDS
TCTGGCGGTCGCAGCAGGGA+TGG 0.500920 4_4:+67997236 Msa0697940:CDS
TTGCAGCTTTGAAATCAAAT+GGG 0.504229 4_4:+67997487 Msa0697940:intron
TCTTCATGGGGAATATATGA+AGG 0.505584 4_4:+67998259 Msa0697940:three_prime_UTR
TATGGTGGAAGTGGTGGTGG+TGG 0.507336 4_4:+67997179 Msa0697940:CDS
GGTAGAGAAAGTAGATATGG+TGG 0.507553 4_4:+67997164 Msa0697940:CDS
CATGCATGTCGAGTGCTGCT+TGG 0.509513 4_4:+67997753 Msa0697940:three_prime_UTR
CAAACCACATTTCTATGTGC+TGG 0.509981 4_4:+67998752 Msa0697940:three_prime_UTR
AACCATGTCGTGGAAGAGAA+TGG 0.511194 4_4:-67998491 None:intergenic
ATTAAATACCTCGATGAGAA+AGG 0.513257 4_4:-67997360 None:intergenic
AAATTCTCTGGACGCTCTCG+TGG 0.522134 4_4:+67996843 Msa0697940:CDS
TGTGCCAGAATCATAGAAAA+CGG 0.524140 4_4:-67998287 None:intergenic
GATGCTATTGATGCTATGAA+TGG 0.526053 4_4:+67996906 Msa0697940:CDS
TATCGGATGTTGACCAAGCA+AGG 0.526702 4_4:-67996464 None:intergenic
ATGCGTTATTGTTCTATTTG+GGG 0.526965 4_4:+67998357 Msa0697940:three_prime_UTR
TGTATCCCTTAACTTTCTAT+CGG 0.530467 4_4:-67996481 None:intergenic
ATGCTATCAATAATTGCTGC+TGG 0.532558 4_4:+67997708 Msa0697940:three_prime_UTR
ACGGTAGTCAAACCTGAGCC+TGG 0.533062 4_4:+67998458 Msa0697940:three_prime_UTR
TGCTTCAATTGTGGTAAACC+TGG 0.534286 4_4:+67997080 Msa0697940:CDS
TTCCATTCTCTTCCACGACA+TGG 0.538850 4_4:+67998489 Msa0697940:three_prime_UTR
GGAAGTTTAGGACCAGGCTC+AGG 0.543204 4_4:-67998470 None:intergenic
GGGAGTGCCCTAGTGGTGAA+GGG 0.544084 4_4:+67997114 Msa0697940:CDS
TTCTTCTGGCGGTCGCAGCA+GGG 0.544328 4_4:+67997232 Msa0697940:CDS
CACGATCGCGACCACGCTCT+CGG 0.544700 4_4:-67997010 None:intergenic
TGGCAAGCTTACAGAGGCCA+AGG 0.548700 4_4:+67996515 Msa0697940:CDS
TATCGTGATCGTGATCGTGC+TGG 0.552506 4_4:+67997293 Msa0697940:CDS
GCAGGATTCTTGGCTAAATG+TGG 0.559112 4_4:+67998103 Msa0697940:intron
CTTCATGGGGAATATATGAA+GGG 0.560134 4_4:+67998260 Msa0697940:three_prime_UTR
GGCGGTCGCAGCAGGGATGG+TGG 0.563764 4_4:+67997239 Msa0697940:CDS
ACAAACCAAAAGACCAGCAT+AGG 0.565468 4_4:-67997636 None:intergenic
TGCGTTATTGTTCTATTTGG+GGG 0.565803 4_4:+67998358 Msa0697940:three_prime_UTR
AGCAGGGATGGTGGTAGTCG+TGG 0.574692 4_4:+67997248 Msa0697940:CDS
TTTATGCATGTCGAGTTGCA+TGG 0.575230 4_4:+67997796 Msa0697940:three_prime_UTR
AAAATGATCAAATCTAACAT+TGG 0.578255 4_4:-67998666 None:intergenic
CACTCCCTAGCAAAATGACC+AGG 0.578775 4_4:-67997098 None:intergenic
GGTGGTCGAGGATCTAATGA+TGG 0.580077 4_4:+67997053 Msa0697940:CDS
GGAGTGCCCTAGTGGTGAAG+GGG 0.582980 4_4:+67997115 Msa0697940:CDS
AGTAGATATGGTGGAAGTGG+TGG 0.583787 4_4:+67997173 Msa0697940:CDS
CTTTGGGAAAGTAGTCAAGC+GGG 0.590635 4_4:+67998001 Msa0697940:three_prime_UTR
ATGCTATTGATGCTATGAAT+GGG 0.592487 4_4:+67996907 Msa0697940:CDS
CATTAGAACATTGAATTCAA+CGG 0.594184 4_4:-67997676 None:intergenic
TTTGCTAGGGAGTGCCCTAG+TGG 0.594989 4_4:+67997107 Msa0697940:CDS
TTTGTGGCTTTGGATGTTTG+GGG 0.601798 4_4:+67997870 Msa0697940:three_prime_UTR
GAACGACGTGGATCTGGAGG+CGG 0.604365 4_4:+67997323 Msa0697940:CDS
AACGTTAATTAGTCTAACAA+AGG 0.610574 4_4:-67997613 None:intergenic
GAGAATGGAAGTTTAGGACC+AGG 0.613697 4_4:-67998476 None:intergenic
ATATGATGAAAAGAAAGCAA+TGG 0.616878 4_4:+67996881 Msa0697940:CDS
AATAGTGCGAATATTTAGCA+AGG 0.619579 4_4:-67998545 None:intergenic
CCAAGTTCCGCCCTAACTCA+CGG 0.631741 4_4:+67995672 Msa0697940:five_prime_UTR
GAGTGCCCTAGTGGTGAAGG+GGG 0.631815 4_4:+67997116 Msa0697940:CDS
GGTGATCGCTACCGAGAGCG+TGG 0.633883 4_4:+67996999 Msa0697940:CDS
TTCTTCTCATTGGAAGAACG+AGG 0.636743 4_4:+67997936 Msa0697940:three_prime_UTR
TCTCTAGAGGATCCTTGTTG+AGG 0.640001 4_4:-67996972 None:intergenic
TTCATGGGGAATATATGAAG+GGG 0.640366 4_4:+67998261 Msa0697940:three_prime_UTR
TATGAACGACGTGGATCTGG+AGG 0.640937 4_4:+67997320 Msa0697940:CDS
GATAGAGCTCAGCCTCAACA+AGG 0.641644 4_4:+67996960 Msa0697940:CDS
GCGATCACCATCATCTCTAG+AGG 0.642537 4_4:-67996985 None:intergenic
CATTAGATCCTCGACCACCT+CGG 0.645981 4_4:-67997049 None:intergenic
AATGGAGATCGTTCTTCTGG+CGG 0.649243 4_4:+67997221 Msa0697940:CDS
AACAAACAAAACATCACATG+CGG 0.657583 4_4:-67998410 None:intergenic
AAGAGTACCGTGAGTTAGGG+CGG 0.658136 4_4:-67995679 None:intergenic
TTGATATACCTTTCTCATCG+AGG 0.661732 4_4:+67997352 Msa0697940:exon
GATGGAGGATGCTTCAATTG+TGG 0.663732 4_4:+67997071 Msa0697940:CDS
AAAGTTTGGCAAGCTTACAG+AGG 0.664669 4_4:+67996509 Msa0697940:CDS
CCCCATGAAGAAAACAAGAG+CGG 0.666738 4_4:-67998247 None:intergenic
TCTTTGGGAAAGTAGTCAAG+CGG 0.667376 4_4:+67998000 Msa0697940:three_prime_UTR
GGTCGAGGATCTAATGATGG+AGG 0.667781 4_4:+67997056 Msa0697940:CDS
AACTCCAGCACATAGAAATG+TGG 0.671634 4_4:-67998756 None:intergenic
GCAGTTTAAAATGTCAGACG+AGG 0.679910 4_4:+67996416 Msa0697940:intron
TCATGGGGAATATATGAAGG+GGG 0.691942 4_4:+67998262 Msa0697940:three_prime_UTR
TAGTGAACTCAACCATGTCG+TGG 0.697075 4_4:-67998501 None:intergenic
CGTGATCGAGATTACCGAGG+TGG 0.697615 4_4:+67997035 Msa0697940:CDS
ATAGAATCAAGGTAATCCCG+CGG 0.718822 4_4:-67997827 None:intergenic
GCTGGACCATATGAACGACG+TGG 0.755617 4_4:+67997311 Msa0697940:CDS
GAACGTGATCGAGATTACCG+AGG 0.756337 4_4:+67997032 Msa0697940:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTGTATTTTTTATTTTATTT+TGG + chr4_4:67997383-67997402 Msa0697940:intron 10.0%
!! TATGATAGTAAATAACTAAA+GGG - chr4_4:67996232-67996251 None:intergenic 15.0%
!! TTATGATAGTAAATAACTAA+AGG - chr4_4:67996233-67996252 None:intergenic 15.0%
!! TTACTTGATTTACTAATAAA+AGG - chr4_4:67996692-67996711 None:intergenic 15.0%
!! CAATGTAATATGATAAAAAA+TGG + chr4_4:67996729-67996748 Msa0697940:intron 15.0%
!!! TTTCATTTTATTTTGTGAAA+AGG + chr4_4:67996809-67996828 Msa0697940:intron 15.0%
!! TTAAAAATCGGATTTAAATC+AGG - chr4_4:67995773-67995792 None:intergenic 20.0%
!!! GTTAAAAATTCAAACTTTTG+AGG - chr4_4:67996107-67996126 None:intergenic 20.0%
!! ATAACATAAGAATTAGTACA+TGG - chr4_4:67996395-67996414 None:intergenic 20.0%
!!! CTTTTGTTCATTTTTATTTG+AGG + chr4_4:67996642-67996661 Msa0697940:intron 20.0%
!! TCTGAACATTATATTTGATT+TGG + chr4_4:67996762-67996781 Msa0697940:intron 20.0%
!!! ATTATATTTGATTTGGAGAA+TGG + chr4_4:67996769-67996788 Msa0697940:intron 20.0%
!!! TTTATTTTATTTTGGACGAA+TGG + chr4_4:67997391-67997410 Msa0697940:intron 20.0%
!! CGAAAAAAATACAATTTGAA+AGG - chr4_4:67998631-67998650 None:intergenic 20.0%
!! AAAATGATCAAATCTAACAT+TGG - chr4_4:67998669-67998688 None:intergenic 20.0%
! AAGAAAAAAGTGGAAGAATT+TGG - chr4_4:67995705-67995724 None:intergenic 25.0%
! ATGAAGAATGAAGAAAAAAG+TGG - chr4_4:67995715-67995734 None:intergenic 25.0%
!! GTTTTGTATAATTCGTGTTT+GGG + chr4_4:67995820-67995839 Msa0697940:intron 25.0%
!! TTCTATTAGCTTATTGTTTG+AGG + chr4_4:67996303-67996322 Msa0697940:intron 25.0%
!!! AAAGATAAAGTTTGTTGTCT+AGG - chr4_4:67996626-67996645 None:intergenic 25.0%
!!! CATTTTATTTTGTGAAAAGG+TGG + chr4_4:67996812-67996831 Msa0697940:intron 25.0%
! ATATGATGAAAAGAAAGCAA+TGG + chr4_4:67996881-67996900 Msa0697940:CDS 25.0%
! TATCAAAACACTAGTATCAT+TGG - chr4_4:67997579-67997598 None:intergenic 25.0%
!! AACGTTAATTAGTCTAACAA+AGG - chr4_4:67997616-67997635 None:intergenic 25.0%
! CATTAGAACATTGAATTCAA+CGG - chr4_4:67997679-67997698 None:intergenic 25.0%
! TACCTTGATTCTATTTAAAG+AGG + chr4_4:67997836-67997855 Msa0697940:three_prime_UTR 25.0%
! TTCCTCTTTAAATAGAATCA+AGG - chr4_4:67997841-67997860 None:intergenic 25.0%
! GATTCTATTTAAAGAGGAAT+TGG + chr4_4:67997842-67997861 Msa0697940:three_prime_UTR 25.0%
!! TTAAAAGTATAATGCCGATT+TGG + chr4_4:67997961-67997980 Msa0697940:three_prime_UTR 25.0%
!! TATTGATGTTATTGAGCTTT+TGG + chr4_4:67998081-67998100 Msa0697940:intron 25.0%
! ACAATAACGCATAAAAACAA+TGG - chr4_4:67998349-67998368 None:intergenic 25.0%
! TTATGCGTTATTGTTCTATT+TGG + chr4_4:67998355-67998374 Msa0697940:three_prime_UTR 25.0%
! TATGCGTTATTGTTCTATTT+GGG + chr4_4:67998356-67998375 Msa0697940:three_prime_UTR 25.0%
ATCGGATTTAAATCAGGAAA+AGG - chr4_4:67995767-67995786 None:intergenic 30.0%
GAATTGTCACGGTTAAAAAT+CGG - chr4_4:67995785-67995804 None:intergenic 30.0%
!! GGTTTTGTATAATTCGTGTT+TGG + chr4_4:67995819-67995838 Msa0697940:intron 30.0%
!! TCACTGGTTTACTTGAATTT+AGG + chr4_4:67995848-67995867 Msa0697940:intron 30.0%
! GTTTACTTGAATTTAGGATC+AGG + chr4_4:67995854-67995873 Msa0697940:intron 30.0%
ATATAGAAGATGATCTACGT+AGG - chr4_4:67995965-67995984 None:intergenic 30.0%
GTTATTCCTTTGTAGAGATT+TGG + chr4_4:67996071-67996090 Msa0697940:intron 30.0%
!! ATTTTGAGAGCAATTTGGTT+GGG - chr4_4:67996143-67996162 None:intergenic 30.0%
!! AATTTTGAGAGCAATTTGGT+TGG - chr4_4:67996144-67996163 None:intergenic 30.0%
!! ACGAAATTTTGAGAGCAATT+TGG - chr4_4:67996148-67996167 None:intergenic 30.0%
GTTATGTGTGTTAATTACCT+TGG + chr4_4:67996325-67996344 Msa0697940:intron 30.0%
AACATCCGATAGAAAGTTAA+GGG + chr4_4:67996476-67996495 Msa0697940:CDS 30.0%
TGTATCCCTTAACTTTCTAT+CGG - chr4_4:67996484-67996503 None:intergenic 30.0%
AGGGATACATTTGAAAAGTT+TGG + chr4_4:67996495-67996514 Msa0697940:CDS 30.0%
! ATTTGAGATATTGATCACCT+TGG - chr4_4:67996535-67996554 None:intergenic 30.0%
ATGCTATTGATGCTATGAAT+GGG + chr4_4:67996907-67996926 Msa0697940:CDS 30.0%
ATGAATGGGATAGATTTAGA+TGG + chr4_4:67996921-67996940 Msa0697940:CDS 30.0%
CTCTATCAACAGTAATAGTT+CGG - chr4_4:67996947-67996966 None:intergenic 30.0%
ATTAAATACCTCGATGAGAA+AGG - chr4_4:67997363-67997382 None:intergenic 30.0%
!! TTTGCAGCTTTGAAATCAAA+TGG + chr4_4:67997486-67997505 Msa0697940:intron 30.0%
!! TTGCAGCTTTGAAATCAAAT+GGG + chr4_4:67997487-67997506 Msa0697940:intron 30.0%
!!! GTGTTTTGATATGCAATACT+TGG + chr4_4:67997588-67997607 Msa0697940:three_prime_UTR 30.0%
AAGAAGCAAAACTCTACATT+TGG - chr4_4:67997921-67997940 None:intergenic 30.0%
!! GTCATTTTGGATTCATCAAT+GGG + chr4_4:67998125-67998144 Msa0697940:three_prime_UTR 30.0%
ATGCGTTATTGTTCTATTTG+GGG + chr4_4:67998357-67998376 Msa0697940:three_prime_UTR 30.0%
AACAAACAAAACATCACATG+CGG - chr4_4:67998413-67998432 None:intergenic 30.0%
!! CAGTTTGTTGTGCTATAAAA+CGG + chr4_4:67998439-67998458 Msa0697940:three_prime_UTR 30.0%
AATAGTGCGAATATTTAGCA+AGG - chr4_4:67998548-67998567 None:intergenic 30.0%
! CTTGAATTTAGGATCAGGAT+AGG + chr4_4:67995859-67995878 Msa0697940:intron 35.0%
TATGTGATAGCTGTGAAGTT+TGG + chr4_4:67995982-67996001 Msa0697940:intron 35.0%
TCCTTTGTAGAGATTTGGAA+AGG + chr4_4:67996076-67996095 Msa0697940:intron 35.0%
ACCTTTCCAAATCTCTACAA+AGG - chr4_4:67996080-67996099 None:intergenic 35.0%
CACATTAGCACAAAGTTTGA+TGG + chr4_4:67996200-67996219 Msa0697940:intron 35.0%
! TATACTATTGTGCTGCTGTT+TGG + chr4_4:67996264-67996283 Msa0697940:intron 35.0%
GTGTTGAATTAATCAGTCCA+AGG - chr4_4:67996345-67996364 None:intergenic 35.0%
TTAATTCAACACCGTGTAGT+TGG + chr4_4:67996353-67996372 Msa0697940:intron 35.0%
!! GATGAGTATCGTTGTTTTGT+TGG + chr4_4:67996438-67996457 Msa0697940:CDS 35.0%
CAACATCCGATAGAAAGTTA+AGG + chr4_4:67996475-67996494 Msa0697940:CDS 35.0%
TCATGTTTGTACCTAGAAGT+TGG + chr4_4:67996576-67996595 Msa0697940:intron 35.0%
GATGCTATTGATGCTATGAA+TGG + chr4_4:67996906-67996925 Msa0697940:CDS 35.0%
!! TAAACCTGGTCATTTTGCTA+GGG + chr4_4:67997094-67997113 Msa0697940:CDS 35.0%
TTGATATACCTTTCTCATCG+AGG + chr4_4:67997352-67997371 Msa0697940:exon 35.0%
TTCGTTCACAATGTTCTTCT+TGG + chr4_4:67997536-67997555 Msa0697940:three_prime_UTR 35.0%
! GTTCTTCTTGGATTGTTGTT+TGG + chr4_4:67997548-67997567 Msa0697940:three_prime_UTR 35.0%
!! TTCTTGGATTGTTGTTTGGA+TGG + chr4_4:67997552-67997571 Msa0697940:three_prime_UTR 35.0%
ACTAATTAACGTTCCTATGC+TGG + chr4_4:67997623-67997642 Msa0697940:three_prime_UTR 35.0%
ATGCTATCAATAATTGCTGC+TGG + chr4_4:67997708-67997727 Msa0697940:three_prime_UTR 35.0%
! AGAGGAATTGGAATGTTTTG+TGG + chr4_4:67997854-67997873 Msa0697940:three_prime_UTR 35.0%
! ATTGGAATGTTTTGTGGCTT+TGG + chr4_4:67997860-67997879 Msa0697940:three_prime_UTR 35.0%
!!! TTTTGTGGCTTTGGATGTTT+GGG + chr4_4:67997869-67997888 Msa0697940:three_prime_UTR 35.0%
!!! GGTTTTTTCATGTACCAAGA+TGG + chr4_4:67997891-67997910 Msa0697940:three_prime_UTR 35.0%
! TTTTTCATGTACCAAGATGG+AGG + chr4_4:67997894-67997913 Msa0697940:three_prime_UTR 35.0%
! AGAGTTTTGCTTCTTCTCAT+TGG + chr4_4:67997926-67997945 Msa0697940:three_prime_UTR 35.0%
!! TATCCTGTTTCTGGATCTTT+GGG + chr4_4:67997985-67998004 Msa0697940:three_prime_UTR 35.0%
! TTGGCTAAATGTGGTCATTT+TGG + chr4_4:67998112-67998131 Msa0697940:three_prime_UTR 35.0%
!! GGTCATTTTGGATTCATCAA+TGG + chr4_4:67998124-67998143 Msa0697940:three_prime_UTR 35.0%
CATGTCCAGTTAGCAATAAA+GGG - chr4_4:67998152-67998171 None:intergenic 35.0%
ACATGTCCAGTTAGCAATAA+AGG - chr4_4:67998153-67998172 None:intergenic 35.0%
! AAAATCTTTTCAGCTGCAAG+AGG + chr4_4:67998196-67998215 Msa0697940:three_prime_UTR 35.0%
TCTTCATGGGGAATATATGA+AGG + chr4_4:67998259-67998278 Msa0697940:three_prime_UTR 35.0%
CTTCATGGGGAATATATGAA+GGG + chr4_4:67998260-67998279 Msa0697940:three_prime_UTR 35.0%
TTCATGGGGAATATATGAAG+GGG + chr4_4:67998261-67998280 Msa0697940:three_prime_UTR 35.0%
TGTGCCAGAATCATAGAAAA+CGG - chr4_4:67998290-67998309 None:intergenic 35.0%
TATGATTCTGGCACATTATG+TGG + chr4_4:67998295-67998314 Msa0697940:three_prime_UTR 35.0%
TGCGTTATTGTTCTATTTGG+GGG + chr4_4:67998358-67998377 Msa0697940:three_prime_UTR 35.0%
! CGTTTTATAGCACAACAAAC+TGG - chr4_4:67998441-67998460 None:intergenic 35.0%
TTCGCACTATTTGTCTATCA+TGG + chr4_4:67998557-67998576 Msa0697940:three_prime_UTR 35.0%
ACAATTCGCTCAGGAACATT+AGG + chr4_4:67995798-67995817 Msa0697940:intron 40.0%
! GTACATGGTGTTGAATCAGT+CGG - chr4_4:67996380-67996399 None:intergenic 40.0%
! GCAGTTTAAAATGTCAGACG+AGG + chr4_4:67996416-67996435 Msa0697940:intron 40.0%
!! GAGTATCGTTGTTTTGTTGG+TGG + chr4_4:67996441-67996460 Msa0697940:CDS 40.0%
AAAGTTTGGCAAGCTTACAG+AGG + chr4_4:67996509-67996528 Msa0697940:CDS 40.0%
TGCAGCATAAACCAACTTCT+AGG - chr4_4:67996590-67996609 None:intergenic 40.0%
GTGGTTGTTGACAAATTCTC+TGG + chr4_4:67996831-67996850 Msa0697940:CDS 40.0%
TGCTTCAATTGTGGTAAACC+TGG + chr4_4:67997080-67997099 Msa0697940:CDS 40.0%
!! GTAAACCTGGTCATTTTGCT+AGG + chr4_4:67997093-67997112 Msa0697940:CDS 40.0%
GGTGGTAGAGAAAGTAGATA+TGG + chr4_4:67997161-67997180 Msa0697940:CDS 40.0%
GGTAGAGAAAGTAGATATGG+TGG + chr4_4:67997164-67997183 Msa0697940:CDS 40.0%
! GAAAGTAGATATGGTGGAAG+TGG + chr4_4:67997170-67997189 Msa0697940:CDS 40.0%
CACTATGGTCCAGATAGAAA+TGG + chr4_4:67997203-67997222 Msa0697940:CDS 40.0%
GAACGATCTCCATTTCTATC+TGG - chr4_4:67997215-67997234 None:intergenic 40.0%
AGAAATGGAGATCGTTCTTC+TGG + chr4_4:67997218-67997237 Msa0697940:CDS 40.0%
ACAAACCAAAAGACCAGCAT+AGG - chr4_4:67997639-67997658 None:intergenic 40.0%
! TTTATGCATGTCGAGTTGCA+TGG + chr4_4:67997796-67997815 Msa0697940:three_prime_UTR 40.0%
ATAGAATCAAGGTAATCCCG+CGG - chr4_4:67997830-67997849 None:intergenic 40.0%
!!! GTTTTGTGGCTTTGGATGTT+TGG + chr4_4:67997868-67997887 Msa0697940:three_prime_UTR 40.0%
!! TTTGTGGCTTTGGATGTTTG+GGG + chr4_4:67997870-67997889 Msa0697940:three_prime_UTR 40.0%
TTCTTCTCATTGGAAGAACG+AGG + chr4_4:67997936-67997955 Msa0697940:three_prime_UTR 40.0%
CAGAAACAGGATAGCCAAAT+CGG - chr4_4:67997978-67997997 None:intergenic 40.0%
!! CTATCCTGTTTCTGGATCTT+TGG + chr4_4:67997984-67998003 Msa0697940:three_prime_UTR 40.0%
TTTCCCAAAGATCCAGAAAC+AGG - chr4_4:67997991-67998010 None:intergenic 40.0%
! TCTTTGGGAAAGTAGTCAAG+CGG + chr4_4:67998000-67998019 Msa0697940:three_prime_UTR 40.0%
ACTTCACCATCACTAGAGTT+AGG + chr4_4:67998043-67998062 Msa0697940:intron 40.0%
CTGAAACCTAACTCTAGTGA+TGG - chr4_4:67998052-67998071 None:intergenic 40.0%
! GATGTTATTGAGCTTTTGGC+AGG + chr4_4:67998085-67998104 Msa0697940:intron 40.0%
!!! TACCGCTCTTGTTTTCTTCA+TGG + chr4_4:67998245-67998264 Msa0697940:three_prime_UTR 40.0%
!!! ACCGCTCTTGTTTTCTTCAT+GGG + chr4_4:67998246-67998265 Msa0697940:three_prime_UTR 40.0%
TCATGGGGAATATATGAAGG+GGG + chr4_4:67998262-67998281 Msa0697940:three_prime_UTR 40.0%
! GGTTCCGTTTTCTATGATTC+TGG + chr4_4:67998283-67998302 Msa0697940:three_prime_UTR 40.0%
GTGGAAGAGAATGGAAGTTT+AGG - chr4_4:67998485-67998504 None:intergenic 40.0%
ATGGTTGAGTTCACTAGCAT+TGG + chr4_4:67998508-67998527 Msa0697940:three_prime_UTR 40.0%
CAAACCACATTTCTATGTGC+TGG + chr4_4:67998752-67998771 Msa0697940:three_prime_UTR 40.0%
AACTCCAGCACATAGAAATG+TGG - chr4_4:67998759-67998778 None:intergenic 40.0%
! GAAAATTTTCCTGCAAGCTC+TGG - chr4_4:67998788-67998807 None:intergenic 40.0%
!! TGGAAGAGTACCGTGAGTTA+GGG - chr4_4:67995685-67995704 None:intergenic 45.0%
!! TTGGAAGAGTACCGTGAGTT+AGG - chr4_4:67995686-67995705 None:intergenic 45.0%
TTAACCGTGACAATTCGCTC+AGG + chr4_4:67995789-67995808 Msa0697940:intron 45.0%
TGTTCCTGAGCGAATTGTCA+CGG - chr4_4:67995796-67995815 None:intergenic 45.0%
TCGTGTTTGGGAAACATCAC+TGG + chr4_4:67995832-67995851 Msa0697940:intron 45.0%
AATCAGTCGGTCCAACTACA+CGG - chr4_4:67996367-67996386 None:intergenic 45.0%
TATCGGATGTTGACCAAGCA+AGG - chr4_4:67996467-67996486 None:intergenic 45.0%
! TCTCTAGAGGATCCTTGTTG+AGG - chr4_4:67996975-67996994 None:intergenic 45.0%
! CAAGGATCCTCTAGAGATGA+TGG + chr4_4:67996978-67996997 Msa0697940:CDS 45.0%
GATGGAGGATGCTTCAATTG+TGG + chr4_4:67997071-67997090 Msa0697940:CDS 45.0%
! AGTAGATATGGTGGAAGTGG+TGG + chr4_4:67997173-67997192 Msa0697940:CDS 45.0%
AATGGAGATCGTTCTTCTGG+CGG + chr4_4:67997221-67997240 Msa0697940:CDS 45.0%
!!! ACGTTCCTATGCTGGTCTTT+TGG + chr4_4:67997631-67997650 Msa0697940:three_prime_UTR 45.0%
! GTTGCATGGCATATTTTCCG+CGG + chr4_4:67997810-67997829 Msa0697940:three_prime_UTR 45.0%
! TTGCATGGCATATTTTCCGC+GGG + chr4_4:67997811-67997830 Msa0697940:three_prime_UTR 45.0%
CTACATTTGGACCTCCATCT+TGG - chr4_4:67997908-67997927 None:intergenic 45.0%
! CGATTTGGCTATCCTGTTTC+TGG + chr4_4:67997976-67997995 Msa0697940:three_prime_UTR 45.0%
! CTTTGGGAAAGTAGTCAAGC+GGG + chr4_4:67998001-67998020 Msa0697940:three_prime_UTR 45.0%
! TGAGCTTTTGGCAGGATTCT+TGG + chr4_4:67998093-67998112 Msa0697940:intron 45.0%
GCAGGATTCTTGGCTAAATG+TGG + chr4_4:67998103-67998122 Msa0697940:intron 45.0%
TGGGTCCCTTTATTGCTAAC+TGG + chr4_4:67998144-67998163 Msa0697940:three_prime_UTR 45.0%
CCCCATGAAGAAAACAAGAG+CGG - chr4_4:67998250-67998269 None:intergenic 45.0%
!!! CCGCTCTTGTTTTCTTCATG+GGG + chr4_4:67998247-67998266 Msa0697940:three_prime_UTR 45.0%
CATCACATGCGGCAGTTAAA+AGG - chr4_4:67998402-67998421 None:intergenic 45.0%
! GAGAATGGAAGTTTAGGACC+AGG - chr4_4:67998479-67998498 None:intergenic 45.0%
TTCCATTCTCTTCCACGACA+TGG + chr4_4:67998489-67998508 Msa0697940:three_prime_UTR 45.0%
AACCATGTCGTGGAAGAGAA+TGG - chr4_4:67998494-67998513 None:intergenic 45.0%
TAGTGAACTCAACCATGTCG+TGG - chr4_4:67998504-67998523 None:intergenic 45.0%
!!! ATAATTTTTTTTATTGATTT+AGG + chr4_4:67995916-67995935 Msa0697940:intron 5.0%
!! AAGAGTACCGTGAGTTAGGG+CGG - chr4_4:67995682-67995701 None:intergenic 50.0%
!!! GTTTTGTTGGTGGCCTTGCT+TGG + chr4_4:67996451-67996470 Msa0697940:CDS 50.0%
AAATTCTCTGGACGCTCTCG+TGG + chr4_4:67996843-67996862 Msa0697940:CDS 50.0%
GATAGAGCTCAGCCTCAACA+AGG + chr4_4:67996960-67996979 Msa0697940:CDS 50.0%
GCGATCACCATCATCTCTAG+AGG - chr4_4:67996988-67997007 None:intergenic 50.0%
GAACGTGATCGAGATTACCG+AGG + chr4_4:67997032-67997051 Msa0697940:CDS 50.0%
CATTAGATCCTCGACCACCT+CGG - chr4_4:67997052-67997071 None:intergenic 50.0%
GGTGGTCGAGGATCTAATGA+TGG + chr4_4:67997053-67997072 Msa0697940:CDS 50.0%
GGTCGAGGATCTAATGATGG+AGG + chr4_4:67997056-67997075 Msa0697940:CDS 50.0%
CACTCCCTAGCAAAATGACC+AGG - chr4_4:67997101-67997120 None:intergenic 50.0%
! AGATATGGTGGAAGTGGTGG+TGG + chr4_4:67997176-67997195 Msa0697940:CDS 50.0%
TATCGTGATCGTGATCGTGC+TGG + chr4_4:67997293-67997312 Msa0697940:CDS 50.0%
CCAGATCCACGTCGTTCATA+TGG - chr4_4:67997320-67997339 None:intergenic 50.0%
CCATATGAACGACGTGGATC+TGG + chr4_4:67997317-67997336 Msa0697940:CDS 50.0%
TATGAACGACGTGGATCTGG+AGG + chr4_4:67997320-67997339 Msa0697940:CDS 50.0%
CTGCTCTGCTCTCTCTCTCT+AGG - chr4_4:67995647-67995666 None:intergenic 55.0%
CTAGAGAGAGAGAGCAGAGC+AGG + chr4_4:67995645-67995664 Msa0697940:five_prime_UTR 55.0%
CCAAGTTCCGCCCTAACTCA+CGG + chr4_4:67995672-67995691 Msa0697940:five_prime_UTR 55.0%
TGGCAAGCTTACAGAGGCCA+AGG + chr4_4:67996515-67996534 Msa0697940:CDS 55.0%
! TCTGGACGCTCTCGTGGTTT+TGG + chr4_4:67996849-67996868 Msa0697940:CDS 55.0%
CGTGATCGAGATTACCGAGG+TGG + chr4_4:67997035-67997054 Msa0697940:CDS 55.0%
!! TTTGCTAGGGAGTGCCCTAG+TGG + chr4_4:67997107-67997126 Msa0697940:CDS 55.0%
CTCTTCCCCCTTCACCACTA+GGG - chr4_4:67997124-67997143 None:intergenic 55.0%
CTAGTGGTGAAGGGGGAAGA+GGG + chr4_4:67997123-67997142 Msa0697940:CDS 55.0%
TAGTGGTGAAGGGGGAAGAG+GGG + chr4_4:67997124-67997143 Msa0697940:CDS 55.0%
!! TATGGTGGAAGTGGTGGTGG+TGG + chr4_4:67997179-67997198 Msa0697940:CDS 55.0%
!! AGTGGTGGTGGTGGTCACTA+TGG + chr4_4:67997188-67997207 Msa0697940:CDS 55.0%
GGTGGTAGTCGTGGAGATTC+TGG + chr4_4:67997257-67997276 Msa0697940:CDS 55.0%
GCTGGACCATATGAACGACG+TGG + chr4_4:67997311-67997330 Msa0697940:CDS 55.0%
! CATGCATGTCGAGTGCTGCT+TGG + chr4_4:67997753-67997772 Msa0697940:three_prime_UTR 55.0%
ACGGTAGTCAAACCTGAGCC+TGG + chr4_4:67998458-67998477 Msa0697940:three_prime_UTR 55.0%
! GGAAGTTTAGGACCAGGCTC+AGG - chr4_4:67998473-67998492 None:intergenic 55.0%
CCGTGAGTTAGGGCGGAACT+TGG - chr4_4:67995675-67995694 None:intergenic 60.0%
CGAGATTACCGAGGTGGTCG+AGG + chr4_4:67997041-67997060 Msa0697940:CDS 60.0%
!! AGGGAGTGCCCTAGTGGTGA+AGG + chr4_4:67997113-67997132 Msa0697940:CDS 60.0%
!! GGGAGTGCCCTAGTGGTGAA+GGG + chr4_4:67997114-67997133 Msa0697940:CDS 60.0%
! GGAGTGCCCTAGTGGTGAAG+GGG + chr4_4:67997115-67997134 Msa0697940:CDS 60.0%
GAGTGCCCTAGTGGTGAAGG+GGG + chr4_4:67997116-67997135 Msa0697940:CDS 60.0%
CCTCTTCCCCCTTCACCACT+AGG - chr4_4:67997125-67997144 None:intergenic 60.0%
CCTAGTGGTGAAGGGGGAAG+AGG + chr4_4:67997122-67997141 Msa0697940:CDS 60.0%
AGTGGTGAAGGGGGAAGAGG+GGG + chr4_4:67997125-67997144 Msa0697940:CDS 60.0%
TTCTTCTGGCGGTCGCAGCA+GGG + chr4_4:67997232-67997251 Msa0697940:CDS 60.0%
! AGCAGGGATGGTGGTAGTCG+TGG + chr4_4:67997248-67997267 Msa0697940:CDS 60.0%
GAACGACGTGGATCTGGAGG+CGG + chr4_4:67997323-67997342 Msa0697940:CDS 60.0%
GTTCGTGTGCCAGAGCTTGC+AGG + chr4_4:67998776-67998795 Msa0697940:three_prime_UTR 60.0%
GGTGATCGCTACCGAGAGCG+TGG + chr4_4:67996999-67997018 Msa0697940:CDS 65.0%
CACGATCGCGACCACGCTCT+CGG - chr4_4:67997013-67997032 None:intergenic 65.0%
GTGAAGGGGGAAGAGGGGGA+GGG + chr4_4:67997129-67997148 Msa0697940:CDS 65.0%
TGAAGGGGGAAGAGGGGGAG+GGG + chr4_4:67997130-67997149 Msa0697940:CDS 65.0%
AGAGGGGGAGGGGGAAGGTA+TGG + chr4_4:67997140-67997159 Msa0697940:CDS 65.0%
GTTCTTCTGGCGGTCGCAGC+AGG + chr4_4:67997231-67997250 Msa0697940:CDS 65.0%
GGTGAAGGGGGAAGAGGGGG+AGG + chr4_4:67997128-67997147 Msa0697940:CDS 70.0%
GAAGGGGGAAGAGGGGGAGG+GGG + chr4_4:67997131-67997150 Msa0697940:CDS 70.0%
! GGGGGAGGGGGAAGGTATGG+TGG + chr4_4:67997143-67997162 Msa0697940:CDS 70.0%
TCTGGCGGTCGCAGCAGGGA+TGG + chr4_4:67997236-67997255 Msa0697940:CDS 70.0%
GGGGAAGAGGGGGAGGGGGA+AGG + chr4_4:67997135-67997154 Msa0697940:CDS 75.0%
GGCGGTCGCAGCAGGGATGG+TGG + chr4_4:67997239-67997258 Msa0697940:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 67995631 67998817 67995631 ID=Msa0697940;Name=Msa0697940
chr4_4 mRNA 67995631 67998817 67995631 ID=Msa0697940-mRNA-1;Parent=Msa0697940;Name=Msa0697940-mRNA-1;_AED=0.09;_eAED=0.09;_QI=69|1|1|1|0.5|0.4|5|1259|211
chr4_4 exon 67995631 67995693 67995631 ID=Msa0697940-mRNA-1:exon:20166;Parent=Msa0697940-mRNA-1
chr4_4 exon 67996420 67996536 67996420 ID=Msa0697940-mRNA-1:exon:20167;Parent=Msa0697940-mRNA-1
chr4_4 exon 67996831 67997373 67996831 ID=Msa0697940-mRNA-1:exon:20168;Parent=Msa0697940-mRNA-1
chr4_4 exon 67997493 67998022 67997493 ID=Msa0697940-mRNA-1:exon:20169;Parent=Msa0697940-mRNA-1
chr4_4 exon 67998107 67998817 67998107 ID=Msa0697940-mRNA-1:exon:20170;Parent=Msa0697940-mRNA-1
chr4_4 five_prime_UTR 67995631 67995693 67995631 ID=Msa0697940-mRNA-1:five_prime_utr;Parent=Msa0697940-mRNA-1
chr4_4 five_prime_UTR 67996420 67996425 67996420 ID=Msa0697940-mRNA-1:five_prime_utr;Parent=Msa0697940-mRNA-1
chr4_4 CDS 67996426 67996536 67996426 ID=Msa0697940-mRNA-1:cds;Parent=Msa0697940-mRNA-1
chr4_4 CDS 67996831 67997355 67996831 ID=Msa0697940-mRNA-1:cds;Parent=Msa0697940-mRNA-1
chr4_4 three_prime_UTR 67997356 67997373 67997356 ID=Msa0697940-mRNA-1:three_prime_utr;Parent=Msa0697940-mRNA-1
chr4_4 three_prime_UTR 67997493 67998022 67997493 ID=Msa0697940-mRNA-1:three_prime_utr;Parent=Msa0697940-mRNA-1
chr4_4 three_prime_UTR 67998107 67998817 67998107 ID=Msa0697940-mRNA-1:three_prime_utr;Parent=Msa0697940-mRNA-1
Gene Sequence

>Msa0697940

ATGTCAGACGAGGATGAGTATCGTTGTTTTGTTGGTGGCCTTGCTTGGTCAACATCCGATAGAAAGTTAAGGGATACATTTGAAAAGTTTGGCAAGCTTACAGAGGCCAAGGTGGTTGTTGACAAATTCTCTGGACGCTCTCGTGGTTTTGGATTTGTCACATATGATGAAAAGAAAGCAATGGAAGATGCTATTGATGCTATGAATGGGATAGATTTAGATGGCCGAACTATTACTGTTGATAGAGCTCAGCCTCAACAAGGATCCTCTAGAGATGATGGTGATCGCTACCGAGAGCGTGGTCGCGATCGTGAACGTGATCGAGATTACCGAGGTGGTCGAGGATCTAATGATGGAGGATGCTTCAATTGTGGTAAACCTGGTCATTTTGCTAGGGAGTGCCCTAGTGGTGAAGGGGGAAGAGGGGGAGGGGGAAGGTATGGTGGTAGAGAAAGTAGATATGGTGGAAGTGGTGGTGGTGGTCACTATGGTCCAGATAGAAATGGAGATCGTTCTTCTGGCGGTCGCAGCAGGGATGGTGGTAGTCGTGGAGATTCTGGAAGTGATCGATACTATCGTGATCGTGATCGTGCTGGACCATATGAACGACGTGGATCTGGAGGCGGCTATCGTTGA
Protein sequence

>Msa0697940

MSDEDEYRCFVGGLAWSTSDRKLRDTFEKFGKLTEAKVVVDKFSGRSRGFGFVTYDEKKAMEDAIDAMNGIDLDGRTITVDRAQPQQGSSRDDGDRYRERGRDRERDRDYRGGRGSNDGGCFNCGKPGHFARECPSGEGGRGGGGRYGGRESRYGGSGGGGHYGPDRNGDRSSGGRSRDGGSRGDSGSDRYYRDRDRAGPYERRGSGGGYR*