AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0562480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562480 MtrunA17_Chr8g0375771 97.076 342 10 0 1 342 114 455 0.0 682
Msa0562480 MtrunA17_Chr5g0403581 44.218 294 148 6 47 329 77 365 4.10e-78 243
Msa0562480 MtrunA17_Chr8g0373601 41.695 295 152 8 48 329 79 366 3.25e-74 233
Msa0562480 MtrunA17_Chr5g0444251 31.674 221 133 4 53 263 46 258 8.33e-24 99.4
Msa0562480 MtrunA17_Chr2g0313871 24.771 218 147 6 58 268 110 317 3.66e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562480 AT4G38460.1 64.948 291 92 2 45 335 42 322 2.75e-131 378
Msa0562480 AT4G36810.1 44.554 303 148 7 42 329 68 365 7.31e-82 253
Msa0562480 AT2G18620.1 39.093 353 187 9 1 333 1 345 7.28e-81 250
Msa0562480 AT2G18640.1 43.333 300 150 10 48 334 79 371 1.67e-71 227
Msa0562480 AT2G23800.1 40.667 300 158 10 48 334 83 375 2.16e-66 214
Msa0562480 AT3G20160.1 39.677 310 160 9 43 333 41 342 4.23e-66 212
Msa0562480 AT3G14550.3 42.424 297 150 7 48 329 64 354 2.86e-64 208
Msa0562480 AT3G14550.1 42.424 297 150 7 48 329 64 354 2.86e-64 208
Msa0562480 AT3G14550.2 42.424 297 150 7 48 329 64 354 2.86e-64 208
Msa0562480 AT3G20160.2 39.535 301 145 9 43 333 41 314 1.94e-63 204
Msa0562480 AT3G14530.1 42.761 297 149 7 48 329 64 354 3.83e-63 205
Msa0562480 AT3G14510.1 42.599 277 138 7 68 329 8 278 9.68e-63 201
Msa0562480 AT3G29430.1 41.231 325 166 11 24 329 33 351 6.11e-62 202
Msa0562480 AT3G32040.1 42.712 295 152 7 48 329 64 354 4.00e-61 200
Msa0562480 AT1G49530.1 39.672 305 157 8 52 333 34 334 6.17e-60 196
Msa0562480 AT1G17050.1 26.490 151 102 3 119 268 171 313 1.58e-11 65.5

Find 143 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGTTATGTAGAGGTTTA+TGG 0.097267 4_1:+62426542 Msa0562480:CDS
TCTGATTAATCTGCCAAATT+AGG 0.105478 4_1:-62425274 None:intergenic
CGTATTGGACTTCCCTAATT+TGG 0.207587 4_1:+62425261 Msa0562480:CDS
AGAGATTACCGATATGAAAA+TGG 0.230892 4_1:-62424642 None:intergenic
TCAACCACTTATAGTTCTTT+TGG 0.249081 4_1:+62426736 Msa0562480:three_prime_UTR
TTGGATGGATTTGAGAAGTA+TGG 0.258489 4_1:+62426620 Msa0562480:CDS
AGCAAAGGCTTTGGAGGAAT+TGG 0.259488 4_1:+62426601 Msa0562480:CDS
CGGTGTTGACATGGCAATTC+TGG 0.263672 4_1:+62426145 Msa0562480:CDS
AAATACAAACATGCACTTAT+TGG 0.264686 4_1:+62426871 Msa0562480:three_prime_UTR
TGCTATTGTAGATGATATTT+TGG 0.266530 4_1:+62426472 Msa0562480:CDS
TGTATCAATCCAACTGCATT+TGG 0.293189 4_1:-62426329 None:intergenic
GGCTCCACTGGAATGGCTGC+AGG 0.305044 4_1:+62426281 Msa0562480:CDS
AATGGCTGCAGGGCAGTTCT+TGG 0.308107 4_1:+62426292 Msa0562480:CDS
AGAGTTATGTAGAGGTTTAT+GGG 0.313757 4_1:+62426543 Msa0562480:CDS
CTGATTAATCTGCCAAATTA+GGG 0.317142 4_1:-62425273 None:intergenic
GGCTGCTCCTTCTCCTTCTT+CGG 0.326678 4_1:+62425199 Msa0562480:CDS
GGCATTGTCGCAATTGTAAA+AGG 0.335180 4_1:-62425113 None:intergenic
GGGCAGTTCTTGGACCTTGA+AGG 0.335310 4_1:+62426302 Msa0562480:CDS
CGTAATTCACAAAACTATAG+AGG 0.352365 4_1:-62426658 None:intergenic
GGCAGTTCTTGGACCTTGAA+GGG 0.352960 4_1:+62426303 Msa0562480:CDS
GGATAAGATTCGGAGACTTT+CGG 0.378999 4_1:-62425157 None:intergenic
TGCCATGTCAACACCGTAGA+TGG 0.385514 4_1:-62426138 None:intergenic
CCGTAGATGGCGTGGTTTGA+GGG 0.385791 4_1:-62426125 None:intergenic
TCTAAAATGCAATATTCATA+AGG 0.392996 4_1:-62426897 None:intergenic
CAAGCAGAGATACACATAAC+AGG 0.399894 4_1:-62425389 None:intergenic
GAGATTACCGATATGAAAAT+GGG 0.401571 4_1:-62424641 None:intergenic
TTCCCATCTTTATCTCCCTC+AGG 0.406329 4_1:-62426509 None:intergenic
ACTTTGATTTGAAGACGTAT+TGG 0.408292 4_1:+62425246 Msa0562480:CDS
ACACGAAGTACACGGGGTTC+AGG 0.414466 4_1:-62426233 None:intergenic
GTATCAATCCAACTGCATTT+GGG 0.418171 4_1:-62426328 None:intergenic
ATAGAGAGACTGAGGAGGTA+TGG 0.421597 4_1:+62426428 Msa0562480:CDS
AGAACTTAGAGCAAAGGCTT+TGG 0.423144 4_1:+62426592 Msa0562480:CDS
GCCCGCTGTGTGGGCTCCAC+TGG 0.424889 4_1:+62426269 Msa0562480:CDS
TGGGGTTAATTAAGAGAGTA+AGG 0.429252 4_1:+62426945 Msa0562480:three_prime_UTR
GAAAGAAGATTACCATTTCA+AGG 0.432327 4_1:-62425460 None:intergenic
AGCACGGGACTAGTCCTCTT+TGG 0.433750 4_1:-62427046 None:intergenic
GCAGCCATGGAGCAGCGAAT+TGG 0.434085 4_1:-62425182 None:intergenic
GGTGATGCACTCTTTCCCCT+TGG 0.434286 4_1:+62426170 Msa0562480:CDS
TGCAGTGTGTGGAGGGTTGT+TGG 0.437777 4_1:+62426385 Msa0562480:CDS
GTGAAATGGCGTGACTGTAC+TGG 0.439817 4_1:-62424599 None:intergenic
TGGCGTGACTGTACTGGGTT+AGG 0.446821 4_1:-62424593 None:intergenic
AGATGGCGTGGTTTGAGGGC+TGG 0.460200 4_1:-62426121 None:intergenic
ACTTAGAGCAAAGGCTTTGG+AGG 0.466634 4_1:+62426595 Msa0562480:CDS
CGAATTGGAAGGATAAGATT+CGG 0.471835 4_1:-62425167 None:intergenic
TTGAGGGAACTGAACTTCAA+TGG 0.473114 4_1:-62425309 None:intergenic
CTGTGTGGGCTCCACTGGAA+TGG 0.474784 4_1:+62426274 Msa0562480:CDS
TCTCTCTATCTCATCATCTT+CGG 0.478860 4_1:-62426414 None:intergenic
GCAGTTCTTGGACCTTGAAG+GGG 0.479193 4_1:+62426304 Msa0562480:CDS
CACTGCATGTGCCCTTGAAA+TGG 0.480852 4_1:+62425448 Msa0562480:CDS
CGGTATTCTGCCCTTGCTAA+AGG 0.483034 4_1:+62425350 Msa0562480:CDS
AAACCTGAGGGAGATAAAGA+TGG 0.484680 4_1:+62426506 Msa0562480:CDS
CAATGCCCTCATTAAACATC+GGG 0.486811 4_1:+62425129 Msa0562480:CDS
TCCAGTGGAGCCCACACAGC+GGG 0.487936 4_1:-62426270 None:intergenic
CAGTGAGTTTGGCGAAATCA+TGG 0.490226 4_1:+62426764 Msa0562480:three_prime_UTR
ATTTAGAGGTGGGGCAGTAA+TGG 0.490613 4_1:+62427020 Msa0562480:three_prime_UTR
TTGACGAGGCCAGTAGATGA+TGG 0.492007 4_1:+62426702 Msa0562480:CDS
ACCGTAGATGGCGTGGTTTG+AGG 0.494704 4_1:-62426126 None:intergenic
CCCTCAAACCACGCCATCTA+CGG 0.495109 4_1:+62426125 Msa0562480:CDS
AGAAGGAGAAGGAGCAGCCA+TGG 0.495191 4_1:-62425195 None:intergenic
AGGAGGTATGGTAGAGCTGT+TGG 0.495677 4_1:+62426440 Msa0562480:CDS
CGTGGTTTGAGGGCTGGCCA+CGG 0.508134 4_1:-62426115 None:intergenic
CAGTTCTTGGACCTTGAAGG+GGG 0.508920 4_1:+62426305 Msa0562480:CDS
ATGAAAATGGGGTTGTGATA+AGG 0.509752 4_1:-62424629 None:intergenic
CCTTCCAATTCGCTGCTCCA+TGG 0.517982 4_1:+62425178 Msa0562480:CDS
GTGTGTGGAGGGTTGTTGGC+CGG 0.518361 4_1:+62426389 Msa0562480:CDS
ATCGCTTAGCACCTTTAGCA+AGG 0.521626 4_1:-62425361 None:intergenic
TATCTCATCATCTTCGGCAC+CGG 0.522001 4_1:-62426408 None:intergenic
CGCCATCTACGGTGTTGACA+TGG 0.522018 4_1:+62426136 Msa0562480:CDS
ATGATGAGATAGAGAGACTG+AGG 0.525344 4_1:+62426420 Msa0562480:CDS
TTCAAGAACCCATCATCTAC+TGG 0.527028 4_1:-62426711 None:intergenic
TCGTCCAAAAGAACTATAAG+TGG 0.527126 4_1:-62426740 None:intergenic
GCTCCACTGGAATGGCTGCA+GGG 0.527665 4_1:+62426282 Msa0562480:CDS
GTAATTCACAAAACTATAGA+GGG 0.531875 4_1:-62426657 None:intergenic
GGGCTGGCCACGGCGTGACA+AGG 0.532295 4_1:-62426105 None:intergenic
TGACAAGGAGTCATCCATGC+AGG 0.533253 4_1:-62426090 None:intergenic
ATAGCTTCATATATCTGTTG+AGG 0.536580 4_1:-62425326 None:intergenic
CAGAGGGAAGAGTTATGTAG+AGG 0.537549 4_1:+62426535 Msa0562480:CDS
GATCGGATGGAAACCGAAGA+AGG 0.542003 4_1:-62425212 None:intergenic
GAAACACCCGATGTTTAATG+AGG 0.542457 4_1:-62425135 None:intergenic
GAGTTATGTAGAGGTTTATG+GGG 0.542842 4_1:+62426544 Msa0562480:CDS
GATAAGGTGGTAATGTGAAA+TGG 0.543730 4_1:-62424613 None:intergenic
AACAATGTGTTGAAATCCAA+GGG 0.546111 4_1:-62426186 None:intergenic
CATTTCAAGGGCACATGCAG+TGG 0.547793 4_1:-62425447 None:intergenic
TAGCTTCATATATCTGTTGA+GGG 0.547993 4_1:-62425325 None:intergenic
AAGGCTTTGGAGGAATTGGA+TGG 0.548932 4_1:+62426605 Msa0562480:CDS
GACTGTACTGGGTTAGGCAT+TGG 0.551081 4_1:-62424587 None:intergenic
GAGTGTTCTGCAGTGTGTGG+AGG 0.552684 4_1:+62426377 Msa0562480:CDS
AAGGGCACATGCAGTGGGGA+AGG 0.558957 4_1:-62425441 None:intergenic
AAAGAAGATTACCATTTCAA+GGG 0.560180 4_1:-62425459 None:intergenic
GTACACGGGGTTCAGGTACA+AGG 0.565661 4_1:-62426226 None:intergenic
AGAAATTGTCATAATCAGAG+TGG 0.568045 4_1:-62426844 None:intergenic
ATCATCATGTATCAATGAAG+CGG 0.568247 4_1:-62426063 None:intergenic
ATGCAGTGGGGAAGGCAGCA+AGG 0.568393 4_1:-62425433 None:intergenic
AACCTGAGGGAGATAAAGAT+GGG 0.571753 4_1:+62426507 Msa0562480:CDS
ATGAGATAGAGAGACTGAGG+AGG 0.573192 4_1:+62426423 Msa0562480:CDS
ATTTCAAGGGCACATGCAGT+GGG 0.576737 4_1:-62425446 None:intergenic
CAGCAATGACACGAAGTACA+CGG 0.576879 4_1:-62426241 None:intergenic
AGTGTTCTGCAGTGTGTGGA+GGG 0.579045 4_1:+62426378 Msa0562480:CDS
GGCTGAAGAACTTAGAGCAA+AGG 0.579804 4_1:+62426586 Msa0562480:CDS
CTGCCCTGCAGCCATTCCAG+TGG 0.580181 4_1:-62426285 None:intergenic
GCTGAGATAGCCCGCTGTGT+GGG 0.580286 4_1:+62426260 Msa0562480:CDS
AGCAATGACACGAAGTACAC+GGG 0.582072 4_1:-62426240 None:intergenic
TGAAATGGCGTGACTGTACT+GGG 0.582756 4_1:-62424598 None:intergenic
GTTGACATGGCAATTCTGGC+GGG 0.592172 4_1:+62426149 Msa0562480:CDS
GACTGTGAATCAATCTAAAG+CGG 0.594820 4_1:+62426982 Msa0562480:three_prime_UTR
TCGCTTAGCACCTTTAGCAA+GGG 0.604739 4_1:-62425360 None:intergenic
GAAATTGTCATAATCAGAGT+GGG 0.607421 4_1:-62426843 None:intergenic
ACATGATGATCTTCCCTGCA+TGG 0.608437 4_1:+62426076 Msa0562480:CDS
TGACGAGGCCAGTAGATGAT+GGG 0.612003 4_1:+62426703 Msa0562480:exon
ACAATGCCCTCATTAAACAT+CGG 0.612387 4_1:+62425128 Msa0562480:CDS
GGGTTCAGGTACAAGGTCAG+AGG 0.613058 4_1:-62426219 None:intergenic
CCATGGAGCAGCGAATTGGA+AGG 0.614799 4_1:-62425178 None:intergenic
ATGGAAACCGAAGAAGGAGA+AGG 0.615829 4_1:-62425206 None:intergenic
TGTTGACATGGCAATTCTGG+CGG 0.616197 4_1:+62426148 Msa0562480:CDS
AAACAATGTGTTGAAATCCA+AGG 0.617990 4_1:-62426187 None:intergenic
AAGCAGAGATACACATAACA+GGG 0.618552 4_1:-62425388 None:intergenic
GGAAGAGAGACTGAAACCTG+AGG 0.622933 4_1:+62426493 Msa0562480:CDS
GATGACTCCTTGTCACGCCG+TGG 0.624688 4_1:+62426098 Msa0562480:CDS
TCTCGTAAAGACTAGAGCAC+GGG 0.626060 4_1:-62427061 None:intergenic
TTCTCGTAAAGACTAGAGCA+CGG 0.630041 4_1:-62427062 None:intergenic
GACAAGGAGTCATCCATGCA+GGG 0.630249 4_1:-62426089 None:intergenic
GCGGAGTGTTCTGCAGTGTG+TGG 0.632086 4_1:+62426374 Msa0562480:CDS
TGCTGAGATAGCCCGCTGTG+TGG 0.636542 4_1:+62426259 Msa0562480:CDS
GAAGAGAGACTGAAACCTGA+GGG 0.638321 4_1:+62426494 Msa0562480:CDS
AAACACCCGATGTTTAATGA+GGG 0.640367 4_1:-62425134 None:intergenic
TGTCAACACCGTAGATGGCG+TGG 0.643803 4_1:-62426133 None:intergenic
AAAATGGGGTTGTGATAAGG+TGG 0.645607 4_1:-62424626 None:intergenic
GGCAGTAATGGATACCAAAG+AGG 0.647150 4_1:+62427032 Msa0562480:three_prime_UTR
GCAGAGATACACATAACAGG+GGG 0.650726 4_1:-62425386 None:intergenic
AGAGATACACATAACAGGGG+GGG 0.650807 4_1:-62425384 None:intergenic
TAGAAGTTTCAATGTTGACG+AGG 0.655023 4_1:+62426688 Msa0562480:CDS
GGTTCAGGTACAAGGTCAGA+GGG 0.665434 4_1:-62426218 None:intergenic
AGATTACCGATATGAAAATG+GGG 0.687205 4_1:-62424640 None:intergenic
AACAGATATATGAAGCTATG+CGG 0.703508 4_1:+62425330 Msa0562480:CDS
TAATTCACAAAACTATAGAG+GGG 0.709745 4_1:-62426656 None:intergenic
AGCAGAGATACACATAACAG+GGG 0.710591 4_1:-62425387 None:intergenic
TTCCAGTGGAGCCCACACAG+CGG 0.722527 4_1:-62426271 None:intergenic
ACAATGTGTTGAAATCCAAG+GGG 0.726426 4_1:-62426185 None:intergenic
TGAGTTTGGCGAAATCATGG+TGG 0.733549 4_1:+62426767 Msa0562480:three_prime_UTR
CAGAGATACACATAACAGGG+GGG 0.735594 4_1:-62425385 None:intergenic
TTTCAAGGGCACATGCAGTG+GGG 0.742785 4_1:-62425445 None:intergenic
ATAATCAGAGTGGGACACCG+CGG 0.764761 4_1:-62426834 None:intergenic
GCAATGACACGAAGTACACG+GGG 0.769728 4_1:-62426239 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATTATTTGAATTTAGA+TGG + chr4_1:62424799-62424818 Msa0562480:intron 10.0%
!! TGAAATGATTAATTATTAGT+AGG + chr4_1:62424992-62425011 Msa0562480:intron 15.0%
!! GAAATGATTAATTATTAGTA+GGG + chr4_1:62424993-62425012 Msa0562480:intron 15.0%
!! TTAACTCTATAAGAAAAAAA+AGG - chr4_1:62425085-62425104 None:intergenic 15.0%
!!! ATGTATTTTAATATTTTGGT+CGG + chr4_1:62427187-62427206 Msa0562480:three_prime_UTR 15.0%
!!! ATTAGTGTTGAGAATAAAAT+GGG - chr4_1:62424747-62424766 None:intergenic 20.0%
!!! AATTAGTGTTGAGAATAAAA+TGG - chr4_1:62424748-62424767 None:intergenic 20.0%
!!! ATTTATGCAATTTCCAATTT+TGG + chr4_1:62424765-62424784 Msa0562480:intron 20.0%
!!! TTTATGCAATTTCCAATTTT+GGG + chr4_1:62424766-62424785 Msa0562480:intron 20.0%
!! TATTTGAATTTAGATGGAAA+TGG + chr4_1:62424805-62424824 Msa0562480:intron 20.0%
!!! TTTTCTTATAGAGTTAAAGA+TGG + chr4_1:62425088-62425107 Msa0562480:intron 20.0%
!! TTATGTTTGGTATTTCTTAT+TGG + chr4_1:62425921-62425940 Msa0562480:intron 20.0%
!!! ATGATAAACTCTATGAATTT+TGG + chr4_1:62426009-62426028 Msa0562480:intron 20.0%
!! TTGATACAAGATAAAAAGTT+TGG + chr4_1:62426344-62426363 Msa0562480:CDS 20.0%
!!! TCTAAAATGCAATATTCATA+AGG - chr4_1:62426900-62426919 None:intergenic 20.0%
!! AGAGCTGTAAAAAATATAAT+TGG + chr4_1:62426925-62426944 Msa0562480:three_prime_UTR 20.0%
!! GAGCTGTAAAAAATATAATT+GGG + chr4_1:62426926-62426945 Msa0562480:three_prime_UTR 20.0%
!! AGCTGTAAAAAATATAATTG+GGG + chr4_1:62426927-62426946 Msa0562480:three_prime_UTR 20.0%
!!! CTGCATGTATTTTAATATTT+TGG + chr4_1:62427183-62427202 Msa0562480:three_prime_UTR 20.0%
!! TTCATTGTATTTTCTTCTCA+CGG + chr4_1:62424695-62424714 Msa0562480:intron 25.0%
!! TTAGTGTTGAGAATAAAATG+GGG - chr4_1:62424746-62424765 None:intergenic 25.0%
!! TTATGCAATTTCCAATTTTG+GGG + chr4_1:62424767-62424786 Msa0562480:intron 25.0%
! CATTTCATCAAACAATTAGT+GGG - chr4_1:62424914-62424933 None:intergenic 25.0%
! AAAGAAGATTACCATTTCAA+GGG - chr4_1:62425462-62425481 None:intergenic 25.0%
! ACAATAGAATGAATATGAAG+TGG - chr4_1:62425495-62425514 None:intergenic 25.0%
! GATAGATAAAAGTAAAACTC+TGG - chr4_1:62425607-62425626 None:intergenic 25.0%
! AATCAAAATTGGAAAGAGAA+AGG - chr4_1:62425690-62425709 None:intergenic 25.0%
!!! ATAGTACATACTTTGACTTT+TGG + chr4_1:62425847-62425866 Msa0562480:intron 25.0%
! AATATTCAGAGACACATTTA+AGG - chr4_1:62425902-62425921 None:intergenic 25.0%
! TGTCTCTGAATATTTATGTT+TGG + chr4_1:62425908-62425927 Msa0562480:intron 25.0%
!! AGATAAAAAGTTTGGTGAAA+TGG + chr4_1:62426352-62426371 Msa0562480:CDS 25.0%
!! TGCTATTGTAGATGATATTT+TGG + chr4_1:62426472-62426491 Msa0562480:CDS 25.0%
! TAATTCACAAAACTATAGAG+GGG - chr4_1:62426659-62426678 None:intergenic 25.0%
! GTAATTCACAAAACTATAGA+GGG - chr4_1:62426660-62426679 None:intergenic 25.0%
! AAATACAAACATGCACTTAT+TGG + chr4_1:62426871-62426890 Msa0562480:three_prime_UTR 25.0%
!!! CCTCTAAATACTTTTTGAAT+CGG - chr4_1:62427009-62427028 None:intergenic 25.0%
!!! ATTCAAAAAGTATTTAGAGG+TGG + chr4_1:62427009-62427028 Msa0562480:three_prime_UTR 25.0%
!!! TTCAAAAAGTATTTAGAGGT+GGG + chr4_1:62427010-62427029 Msa0562480:three_prime_UTR 25.0%
AGATTACCGATATGAAAATG+GGG - chr4_1:62424643-62424662 None:intergenic 30.0%
GAGATTACCGATATGAAAAT+GGG - chr4_1:62424644-62424663 None:intergenic 30.0%
AGAGATTACCGATATGAAAA+TGG - chr4_1:62424645-62424664 None:intergenic 30.0%
AGTGAATTAGTGTGTTAGAT+AGG + chr4_1:62424873-62424892 Msa0562480:intron 30.0%
GCATTTCATCAAACAATTAG+TGG - chr4_1:62424915-62424934 None:intergenic 30.0%
!! ACTTTGATTTGAAGACGTAT+TGG + chr4_1:62425246-62425265 Msa0562480:CDS 30.0%
CTGATTAATCTGCCAAATTA+GGG - chr4_1:62425276-62425295 None:intergenic 30.0%
TCTGATTAATCTGCCAAATT+AGG - chr4_1:62425277-62425296 None:intergenic 30.0%
TAGCTTCATATATCTGTTGA+GGG - chr4_1:62425328-62425347 None:intergenic 30.0%
ATAGCTTCATATATCTGTTG+AGG - chr4_1:62425329-62425348 None:intergenic 30.0%
AACAGATATATGAAGCTATG+CGG + chr4_1:62425330-62425349 Msa0562480:CDS 30.0%
GAAAGAAGATTACCATTTCA+AGG - chr4_1:62425463-62425482 None:intergenic 30.0%
CAGATACAACTAGCTAAAAA+TGG - chr4_1:62425821-62425840 None:intergenic 30.0%
!!! CTTATATTTTAGTTGCAAGC+AGG + chr4_1:62426034-62426053 Msa0562480:intron 30.0%
ATCATCATGTATCAATGAAG+CGG - chr4_1:62426066-62426085 None:intergenic 30.0%
AACAATGTGTTGAAATCCAA+GGG - chr4_1:62426189-62426208 None:intergenic 30.0%
AAACAATGTGTTGAAATCCA+AGG - chr4_1:62426190-62426209 None:intergenic 30.0%
! TAAAAAGTTTGGTGAAATGG+CGG + chr4_1:62426355-62426374 Msa0562480:CDS 30.0%
ATAAAGATGGGAAAAACAGA+GGG + chr4_1:62426519-62426538 Msa0562480:CDS 30.0%
AAGAGTTATGTAGAGGTTTA+TGG + chr4_1:62426542-62426561 Msa0562480:CDS 30.0%
AGAGTTATGTAGAGGTTTAT+GGG + chr4_1:62426543-62426562 Msa0562480:CDS 30.0%
CGTAATTCACAAAACTATAG+AGG - chr4_1:62426661-62426680 None:intergenic 30.0%
! TCAACCACTTATAGTTCTTT+TGG + chr4_1:62426736-62426755 Msa0562480:three_prime_UTR 30.0%
GAAATTGTCATAATCAGAGT+GGG - chr4_1:62426846-62426865 None:intergenic 30.0%
AGAAATTGTCATAATCAGAG+TGG - chr4_1:62426847-62426866 None:intergenic 30.0%
!! CCGATTCAAAAAGTATTTAG+AGG + chr4_1:62427006-62427025 Msa0562480:three_prime_UTR 30.0%
!! TCAAAAAGTATTTAGAGGTG+GGG + chr4_1:62427011-62427030 Msa0562480:three_prime_UTR 30.0%
! GATAAGGTGGTAATGTGAAA+TGG - chr4_1:62424616-62424635 None:intergenic 35.0%
ATGAAAATGGGGTTGTGATA+AGG - chr4_1:62424632-62424651 None:intergenic 35.0%
! TCACAACCCCATTTTCATAT+CGG + chr4_1:62424634-62424653 Msa0562480:five_prime_UTR 35.0%
ACAATGCCCTCATTAAACAT+CGG + chr4_1:62425128-62425147 Msa0562480:CDS 35.0%
AAACACCCGATGTTTAATGA+GGG - chr4_1:62425137-62425156 None:intergenic 35.0%
CGAATTGGAAGGATAAGATT+CGG - chr4_1:62425170-62425189 None:intergenic 35.0%
! CAAAGTGAACAGCTTTTGAT+CGG - chr4_1:62425232-62425251 None:intergenic 35.0%
AAGCAGAGATACACATAACA+GGG - chr4_1:62425391-62425410 None:intergenic 35.0%
! ATCTCTGCTTGTGAACTTTT+CGG + chr4_1:62425401-62425420 Msa0562480:CDS 35.0%
! GGATGAGCTTTCTTTTTCTT+TGG + chr4_1:62425660-62425679 Msa0562480:intron 35.0%
ACAATGTGTTGAAATCCAAG+GGG - chr4_1:62426188-62426207 None:intergenic 35.0%
GTATCAATCCAACTGCATTT+GGG - chr4_1:62426331-62426350 None:intergenic 35.0%
TGTATCAATCCAACTGCATT+TGG - chr4_1:62426332-62426351 None:intergenic 35.0%
TCTCTCTATCTCATCATCTT+CGG - chr4_1:62426417-62426436 None:intergenic 35.0%
GATAAAGATGGGAAAAACAG+AGG + chr4_1:62426518-62426537 Msa0562480:CDS 35.0%
GAGTTATGTAGAGGTTTATG+GGG + chr4_1:62426544-62426563 Msa0562480:CDS 35.0%
! TTGGATGGATTTGAGAAGTA+TGG + chr4_1:62426620-62426639 Msa0562480:CDS 35.0%
TAGAAGTTTCAATGTTGACG+AGG + chr4_1:62426688-62426707 Msa0562480:CDS 35.0%
TCGTCCAAAAGAACTATAAG+TGG - chr4_1:62426743-62426762 None:intergenic 35.0%
! TGTAGTTTTTGTCAAAACCG+CGG + chr4_1:62426817-62426836 Msa0562480:three_prime_UTR 35.0%
TGGGGTTAATTAAGAGAGTA+AGG + chr4_1:62426945-62426964 Msa0562480:three_prime_UTR 35.0%
GACTGTGAATCAATCTAAAG+CGG + chr4_1:62426982-62427001 Msa0562480:three_prime_UTR 35.0%
AAAATGGGGTTGTGATAAGG+TGG - chr4_1:62424629-62424648 None:intergenic 40.0%
!! TGCAATTTCCAATTTTGGGG+TGG + chr4_1:62424770-62424789 Msa0562480:intron 40.0%
!!! AATTTCCAATTTTGGGGTGG+TGG + chr4_1:62424773-62424792 Msa0562480:intron 40.0%
!!! ATTTCCAATTTTGGGGTGGT+GGG + chr4_1:62424774-62424793 Msa0562480:intron 40.0%
GGCATTGTCGCAATTGTAAA+AGG - chr4_1:62425116-62425135 None:intergenic 40.0%
CAATGCCCTCATTAAACATC+GGG + chr4_1:62425129-62425148 Msa0562480:CDS 40.0%
GAAACACCCGATGTTTAATG+AGG - chr4_1:62425138-62425157 None:intergenic 40.0%
GGATAAGATTCGGAGACTTT+CGG - chr4_1:62425160-62425179 None:intergenic 40.0%
CGTATTGGACTTCCCTAATT+TGG + chr4_1:62425261-62425280 Msa0562480:CDS 40.0%
TTGAGGGAACTGAACTTCAA+TGG - chr4_1:62425312-62425331 None:intergenic 40.0%
AGCAGAGATACACATAACAG+GGG - chr4_1:62425390-62425409 None:intergenic 40.0%
CAAGCAGAGATACACATAAC+AGG - chr4_1:62425392-62425411 None:intergenic 40.0%
GACAATGATAGACCAATCTC+TGG - chr4_1:62425555-62425574 None:intergenic 40.0%
AAAGAAAGCTCATCCTCTCA+CGG - chr4_1:62425655-62425674 None:intergenic 40.0%
ATGATGAGATAGAGAGACTG+AGG + chr4_1:62426420-62426439 Msa0562480:CDS 40.0%
AAACCTGAGGGAGATAAAGA+TGG + chr4_1:62426506-62426525 Msa0562480:CDS 40.0%
AACCTGAGGGAGATAAAGAT+GGG + chr4_1:62426507-62426526 Msa0562480:CDS 40.0%
AGAACTTAGAGCAAAGGCTT+TGG + chr4_1:62426592-62426611 Msa0562480:CDS 40.0%
TTCAAGAACCCATCATCTAC+TGG - chr4_1:62426714-62426733 None:intergenic 40.0%
TTCTCGTAAAGACTAGAGCA+CGG - chr4_1:62427065-62427084 None:intergenic 40.0%
TGAAATGGCGTGACTGTACT+GGG - chr4_1:62424601-62424620 None:intergenic 45.0%
ATGGAAACCGAAGAAGGAGA+AGG - chr4_1:62425209-62425228 None:intergenic 45.0%
! GTGAACAGCTTTTGATCGGA+TGG - chr4_1:62425228-62425247 None:intergenic 45.0%
TCGCTTAGCACCTTTAGCAA+GGG - chr4_1:62425363-62425382 None:intergenic 45.0%
ATCGCTTAGCACCTTTAGCA+AGG - chr4_1:62425364-62425383 None:intergenic 45.0%
AGAGATACACATAACAGGGG+GGG - chr4_1:62425387-62425406 None:intergenic 45.0%
CAGAGATACACATAACAGGG+GGG - chr4_1:62425388-62425407 None:intergenic 45.0%
GCAGAGATACACATAACAGG+GGG - chr4_1:62425389-62425408 None:intergenic 45.0%
! TCTGCTTGTGAACTTTTCGG+AGG + chr4_1:62425404-62425423 Msa0562480:CDS 45.0%
! ATTTCAAGGGCACATGCAGT+GGG - chr4_1:62425449-62425468 None:intergenic 45.0%
ACATGATGATCTTCCCTGCA+TGG + chr4_1:62426076-62426095 Msa0562480:CDS 45.0%
TGTTGACATGGCAATTCTGG+CGG + chr4_1:62426148-62426167 Msa0562480:CDS 45.0%
AGCAATGACACGAAGTACAC+GGG - chr4_1:62426243-62426262 None:intergenic 45.0%
CAGCAATGACACGAAGTACA+CGG - chr4_1:62426244-62426263 None:intergenic 45.0%
TATCTCATCATCTTCGGCAC+CGG - chr4_1:62426411-62426430 None:intergenic 45.0%
ATGAGATAGAGAGACTGAGG+AGG + chr4_1:62426423-62426442 Msa0562480:CDS 45.0%
ATAGAGAGACTGAGGAGGTA+TGG + chr4_1:62426428-62426447 Msa0562480:CDS 45.0%
GAAGAGAGACTGAAACCTGA+GGG + chr4_1:62426494-62426513 Msa0562480:CDS 45.0%
TTCCCATCTTTATCTCCCTC+AGG - chr4_1:62426512-62426531 None:intergenic 45.0%
CAGAGGGAAGAGTTATGTAG+AGG + chr4_1:62426535-62426554 Msa0562480:CDS 45.0%
GGCTGAAGAACTTAGAGCAA+AGG + chr4_1:62426586-62426605 Msa0562480:CDS 45.0%
! ACTTAGAGCAAAGGCTTTGG+AGG + chr4_1:62426595-62426614 Msa0562480:CDS 45.0%
!! AGCAAAGGCTTTGGAGGAAT+TGG + chr4_1:62426601-62426620 Msa0562480:CDS 45.0%
!! AAGGCTTTGGAGGAATTGGA+TGG + chr4_1:62426605-62426624 Msa0562480:CDS 45.0%
! TTTTGGACGAGCAGTGAGTT+TGG + chr4_1:62426753-62426772 Msa0562480:three_prime_UTR 45.0%
CAGTGAGTTTGGCGAAATCA+TGG + chr4_1:62426764-62426783 Msa0562480:three_prime_UTR 45.0%
TGAGTTTGGCGAAATCATGG+TGG + chr4_1:62426767-62426786 Msa0562480:three_prime_UTR 45.0%
ATTTAGAGGTGGGGCAGTAA+TGG + chr4_1:62427020-62427039 Msa0562480:three_prime_UTR 45.0%
!! GGCAGTAATGGATACCAAAG+AGG + chr4_1:62427032-62427051 Msa0562480:three_prime_UTR 45.0%
TCTCGTAAAGACTAGAGCAC+GGG - chr4_1:62427064-62427083 None:intergenic 45.0%
!!! TTATTTTAATAAATCAAAAT+TGG - chr4_1:62425701-62425720 None:intergenic 5.0%
! GACTGTACTGGGTTAGGCAT+TGG - chr4_1:62424590-62424609 None:intergenic 50.0%
GTGAAATGGCGTGACTGTAC+TGG - chr4_1:62424602-62424621 None:intergenic 50.0%
AACACCCACCACCCCAAAAT+TGG - chr4_1:62424781-62424800 None:intergenic 50.0%
GATCGGATGGAAACCGAAGA+AGG - chr4_1:62425215-62425234 None:intergenic 50.0%
CGGTATTCTGCCCTTGCTAA+AGG + chr4_1:62425350-62425369 Msa0562480:CDS 50.0%
! TTTCAAGGGCACATGCAGTG+GGG - chr4_1:62425448-62425467 None:intergenic 50.0%
! CATTTCAAGGGCACATGCAG+TGG - chr4_1:62425450-62425469 None:intergenic 50.0%
CACTGCATGTGCCCTTGAAA+TGG + chr4_1:62425448-62425467 Msa0562480:CDS 50.0%
GCTAAGAACTCGCCAGAGAT+TGG + chr4_1:62425540-62425559 Msa0562480:intron 50.0%
TCACGGTAGGCAGTTAGGAA+CGG - chr4_1:62425638-62425657 None:intergenic 50.0%
AAAGCTCATCCTCTCACGGT+AGG - chr4_1:62425651-62425670 None:intergenic 50.0%
GACAAGGAGTCATCCATGCA+GGG - chr4_1:62426092-62426111 None:intergenic 50.0%
TGACAAGGAGTCATCCATGC+AGG - chr4_1:62426093-62426112 None:intergenic 50.0%
TGCCATGTCAACACCGTAGA+TGG - chr4_1:62426141-62426160 None:intergenic 50.0%
!! CGGTGTTGACATGGCAATTC+TGG + chr4_1:62426145-62426164 Msa0562480:CDS 50.0%
GTTGACATGGCAATTCTGGC+GGG + chr4_1:62426149-62426168 Msa0562480:CDS 50.0%
GGTTCAGGTACAAGGTCAGA+GGG - chr4_1:62426221-62426240 None:intergenic 50.0%
GCAATGACACGAAGTACACG+GGG - chr4_1:62426242-62426261 None:intergenic 50.0%
GGCAGTTCTTGGACCTTGAA+GGG + chr4_1:62426303-62426322 Msa0562480:CDS 50.0%
GCAGTTCTTGGACCTTGAAG+GGG + chr4_1:62426304-62426323 Msa0562480:CDS 50.0%
CAGTTCTTGGACCTTGAAGG+GGG + chr4_1:62426305-62426324 Msa0562480:CDS 50.0%
AGTGTTCTGCAGTGTGTGGA+GGG + chr4_1:62426378-62426397 Msa0562480:CDS 50.0%
! AGGAGGTATGGTAGAGCTGT+TGG + chr4_1:62426440-62426459 Msa0562480:CDS 50.0%
GGAAGAGAGACTGAAACCTG+AGG + chr4_1:62426493-62426512 Msa0562480:CDS 50.0%
TGGGGTTGAAAAAGCAGCAG+AGG + chr4_1:62426562-62426581 Msa0562480:CDS 50.0%
GGTTGAAAAAGCAGCAGAGG+TGG + chr4_1:62426565-62426584 Msa0562480:CDS 50.0%
TTGACGAGGCCAGTAGATGA+TGG + chr4_1:62426702-62426721 Msa0562480:CDS 50.0%
TGACGAGGCCAGTAGATGAT+GGG + chr4_1:62426703-62426722 Msa0562480:exon 50.0%
ATAATCAGAGTGGGACACCG+CGG - chr4_1:62426837-62426856 None:intergenic 50.0%
TGGCGTGACTGTACTGGGTT+AGG - chr4_1:62424596-62424615 None:intergenic 55.0%
CCATGGAGCAGCGAATTGGA+AGG - chr4_1:62425181-62425200 None:intergenic 55.0%
CCTTCCAATTCGCTGCTCCA+TGG + chr4_1:62425178-62425197 Msa0562480:CDS 55.0%
AGAAGGAGAAGGAGCAGCCA+TGG - chr4_1:62425198-62425217 None:intergenic 55.0%
GGCTGCTCCTTCTCCTTCTT+CGG + chr4_1:62425199-62425218 Msa0562480:CDS 55.0%
TCCTAACTGCCTACCGTGAG+AGG + chr4_1:62425639-62425658 Msa0562480:intron 55.0%
TCCTCTCACGGTAGGCAGTT+AGG - chr4_1:62425643-62425662 None:intergenic 55.0%
CCGTAGATGGCGTGGTTTGA+GGG - chr4_1:62426128-62426147 None:intergenic 55.0%
CCCTCAAACCACGCCATCTA+CGG + chr4_1:62426125-62426144 Msa0562480:CDS 55.0%
ACCGTAGATGGCGTGGTTTG+AGG - chr4_1:62426129-62426148 None:intergenic 55.0%
TGTCAACACCGTAGATGGCG+TGG - chr4_1:62426136-62426155 None:intergenic 55.0%
!! CGCCATCTACGGTGTTGACA+TGG + chr4_1:62426136-62426155 Msa0562480:CDS 55.0%
GGTGATGCACTCTTTCCCCT+TGG + chr4_1:62426170-62426189 Msa0562480:CDS 55.0%
GGGTTCAGGTACAAGGTCAG+AGG - chr4_1:62426222-62426241 None:intergenic 55.0%
GTACACGGGGTTCAGGTACA+AGG - chr4_1:62426229-62426248 None:intergenic 55.0%
ACACGAAGTACACGGGGTTC+AGG - chr4_1:62426236-62426255 None:intergenic 55.0%
AATGGCTGCAGGGCAGTTCT+TGG + chr4_1:62426292-62426311 Msa0562480:CDS 55.0%
GGGCAGTTCTTGGACCTTGA+AGG + chr4_1:62426302-62426321 Msa0562480:CDS 55.0%
GAGTGTTCTGCAGTGTGTGG+AGG + chr4_1:62426377-62426396 Msa0562480:CDS 55.0%
TGCAGTGTGTGGAGGGTTGT+TGG + chr4_1:62426385-62426404 Msa0562480:CDS 55.0%
!! AGCACGGGACTAGTCCTCTT+TGG - chr4_1:62427049-62427068 None:intergenic 55.0%
GCAGCCATGGAGCAGCGAAT+TGG - chr4_1:62425185-62425204 None:intergenic 60.0%
ATGCAGTGGGGAAGGCAGCA+AGG - chr4_1:62425436-62425455 None:intergenic 60.0%
! AAGGGCACATGCAGTGGGGA+AGG - chr4_1:62425444-62425463 None:intergenic 60.0%
GATGACTCCTTGTCACGCCG+TGG + chr4_1:62426098-62426117 Msa0562480:CDS 60.0%
AGATGGCGTGGTTTGAGGGC+TGG - chr4_1:62426124-62426143 None:intergenic 60.0%
TGCTGAGATAGCCCGCTGTG+TGG + chr4_1:62426259-62426278 Msa0562480:CDS 60.0%
GCTGAGATAGCCCGCTGTGT+GGG + chr4_1:62426260-62426279 Msa0562480:CDS 60.0%
TTCCAGTGGAGCCCACACAG+CGG - chr4_1:62426274-62426293 None:intergenic 60.0%
CTGTGTGGGCTCCACTGGAA+TGG + chr4_1:62426274-62426293 Msa0562480:CDS 60.0%
GCTCCACTGGAATGGCTGCA+GGG + chr4_1:62426282-62426301 Msa0562480:CDS 60.0%
CTGCATTTGGGCCCCCTTCA+AGG - chr4_1:62426319-62426338 None:intergenic 60.0%
GAAGGGGGCCCAAATGCAGT+TGG + chr4_1:62426320-62426339 Msa0562480:CDS 60.0%
! GCGGAGTGTTCTGCAGTGTG+TGG + chr4_1:62426374-62426393 Msa0562480:CDS 60.0%
GTGTGTGGAGGGTTGTTGGC+CGG + chr4_1:62426389-62426408 Msa0562480:CDS 60.0%
!! CGTGGTTTGAGGGCTGGCCA+CGG - chr4_1:62426118-62426137 None:intergenic 65.0%
TCCAGTGGAGCCCACACAGC+GGG - chr4_1:62426273-62426292 None:intergenic 65.0%
GGCTCCACTGGAATGGCTGC+AGG + chr4_1:62426281-62426300 Msa0562480:CDS 65.0%
CTGCCCTGCAGCCATTCCAG+TGG - chr4_1:62426288-62426307 None:intergenic 65.0%
!! GGGCTGGCCACGGCGTGACA+AGG - chr4_1:62426108-62426127 None:intergenic 75.0%
GCCCGCTGTGTGGGCTCCAC+TGG + chr4_1:62426269-62426288 Msa0562480:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 62424571 62427219 62424571 ID=Msa0562480;Name=Msa0562480
chr4_1 mRNA 62424571 62427219 62424571 ID=Msa0562480-mRNA-1;Parent=Msa0562480;Name=Msa0562480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=93|1|1|1|0.5|0.33|3|498|342
chr4_1 exon 62424571 62424655 62424571 ID=Msa0562480-mRNA-1:exon:19710;Parent=Msa0562480-mRNA-1
chr4_1 exon 62425099 62425469 62425099 ID=Msa0562480-mRNA-1:exon:19711;Parent=Msa0562480-mRNA-1
chr4_1 exon 62426056 62427219 62426056 ID=Msa0562480-mRNA-1:exon:19712;Parent=Msa0562480-mRNA-1
chr4_1 five_prime_UTR 62424571 62424655 62424571 ID=Msa0562480-mRNA-1:five_prime_utr;Parent=Msa0562480-mRNA-1
chr4_1 five_prime_UTR 62425099 62425106 62425099 ID=Msa0562480-mRNA-1:five_prime_utr;Parent=Msa0562480-mRNA-1
chr4_1 CDS 62425107 62425469 62425107 ID=Msa0562480-mRNA-1:cds;Parent=Msa0562480-mRNA-1
chr4_1 CDS 62426056 62426721 62426056 ID=Msa0562480-mRNA-1:cds;Parent=Msa0562480-mRNA-1
chr4_1 three_prime_UTR 62426722 62427219 62426722 ID=Msa0562480-mRNA-1:three_prime_utr;Parent=Msa0562480-mRNA-1
Gene Sequence

>Msa0562480

ATGGCTCCTTTTACAATTGCGACAATGCCCTCATTAAACATCGGGTGTTTCCGAAAGTCTCCGAATCTTATCCTTCCAATTCGCTGCTCCATGGCTGCTCCTTCTCCTTCTTCGGTTTCCATCCGATCAAAAGCTGTTCACTTTGATTTGAAGACGTATTGGACTTCCCTAATTTGGCAGATTAATCAGAAGCTCGATGAAGCCATTGAAGTTCAGTTCCCTCAACAGATATATGAAGCTATGCGGTATTCTGCCCTTGCTAAAGGTGCTAAGCGATCCCCCCCTGTTATGTGTATCTCTGCTTGTGAACTTTTCGGAGGCAGTCGCCTTGCTGCCTTCCCCACTGCATGTGCCCTTGAAATGGTTCATGCCGCTTCATTGATACATGATGATCTTCCCTGCATGGATGACTCCTTGTCACGCCGTGGCCAGCCCTCAAACCACGCCATCTACGGTGTTGACATGGCAATTCTGGCGGGTGATGCACTCTTTCCCCTTGGATTTCAACACATTGTTTCACATACTCCCTCTGACCTTGTACCTGAACCCCGTGTACTTCGTGTCATTGCTGAGATAGCCCGCTGTGTGGGCTCCACTGGAATGGCTGCAGGGCAGTTCTTGGACCTTGAAGGGGGCCCAAATGCAGTTGGATTGATACAAGATAAAAAGTTTGGTGAAATGGCGGAGTGTTCTGCAGTGTGTGGAGGGTTGTTGGCCGGTGCCGAAGATGATGAGATAGAGAGACTGAGGAGGTATGGTAGAGCTGTTGGAGTATTGTATGCTATTGTAGATGATATTTTGGAAGAGAGACTGAAACCTGAGGGAGATAAAGATGGGAAAAACAGAGGGAAGAGTTATGTAGAGGTTTATGGGGTTGAAAAAGCAGCAGAGGTGGCTGAAGAACTTAGAGCAAAGGCTTTGGAGGAATTGGATGGATTTGAGAAGTATGGAGATCAAGTGTTGCCCCTCTATAGTTTTGTGAATTACGCTATTGATAGAAGTTTCAATGTTGACGAGGCCAGTAGATGA
Protein sequence

>Msa0562480

MAPFTIATMPSLNIGCFRKSPNLILPIRCSMAAPSPSSVSIRSKAVHFDLKTYWTSLIWQINQKLDEAIEVQFPQQIYEAMRYSALAKGAKRSPPVMCISACELFGGSRLAAFPTACALEMVHAASLIHDDLPCMDDSLSRRGQPSNHAIYGVDMAILAGDALFPLGFQHIVSHTPSDLVPEPRVLRVIAEIARCVGSTGMAAGQFLDLEGGPNAVGLIQDKKFGEMAECSAVCGGLLAGAEDDEIERLRRYGRAVGVLYAIVDDILEERLKPEGDKDGKNRGKSYVEVYGVEKAAEVAEELRAKALEELDGFEKYGDQVLPLYSFVNYAIDRSFNVDEASR*