AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0574620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574620 MtrunA17_Chr8g0391311 94.139 546 27 2 1 546 1 541 0.0 1051
Msa0574620 MtrunA17_Chr8g0391291 93.956 546 26 3 1 546 1 539 0.0 1047
Msa0574620 MtrunA17_Chr8g0391281 92.405 316 17 3 1 316 1 309 0.0 570
Msa0574620 MtrunA17_Chr4g0011771 49.538 541 233 11 13 546 6 513 0.0 520
Msa0574620 MtrunA17_Chr4g0011791 48.885 538 231 13 18 546 7 509 1.10e-176 508
Msa0574620 MtrunA17_Chr4g0018371 44.015 518 265 6 36 546 40 539 2.83e-159 464
Msa0574620 MtrunA17_Chr3g0090091 45.857 519 247 8 36 546 36 528 1.25e-158 462
Msa0574620 MtrunA17_Chr3g0079341 45.385 520 247 7 36 546 36 527 1.02e-157 460
Msa0574620 MtrunA17_Chr4g0011751 51.002 449 190 7 104 546 65 489 8.42e-157 456
Msa0574620 MtrunA17_Chr3g0079361 45.087 519 250 6 36 546 36 527 4.02e-156 456
Msa0574620 MtrunA17_Chr3g0079351 45.385 520 247 9 36 546 36 527 5.56e-155 453
Msa0574620 MtrunA17_Chr7g0269541 49.446 451 209 5 102 542 111 552 1.34e-152 448
Msa0574620 MtrunA17_Chr7g0233771 45.644 528 249 11 37 546 47 554 1.80e-152 448
Msa0574620 MtrunA17_Chr8g0345281 44.612 529 266 7 35 546 71 589 8.79e-149 439
Msa0574620 MtrunA17_Chr3g0126771 42.964 533 267 8 35 546 81 597 1.54e-147 437
Msa0574620 MtrunA17_Chr1g0147051 42.778 540 264 12 35 546 85 607 1.51e-143 427
Msa0574620 MtrunA17_Chr2g0302021 46.751 477 219 7 83 546 71 525 3.05e-142 421
Msa0574620 MtrunA17_Chr7g0233801 42.830 523 260 11 42 546 52 553 1.66e-140 417
Msa0574620 MtrunA17_Chr8g0354901 49.465 467 204 9 92 546 135 581 3.81e-140 417
Msa0574620 MtrunA17_Chr7g0233831 46.957 460 222 7 92 546 113 555 1.66e-139 415
Msa0574620 MtrunA17_Chr7g0272141 44.980 498 235 10 65 546 68 542 1.74e-139 414
Msa0574620 MtrunA17_Chr4g0069841 45.417 480 216 8 85 546 65 516 6.17e-138 409
Msa0574620 MtrunA17_Chr7g0269531 43.333 540 258 10 33 542 40 561 9.61e-137 408
Msa0574620 MtrunA17_Chr1g0192331 44.821 531 241 14 35 546 31 528 2.67e-136 405
Msa0574620 MtrunA17_Chr4g0069891 42.026 533 269 10 33 546 82 593 3.63e-134 402
Msa0574620 MtrunA17_Chr7g0269521 49.184 429 199 6 133 546 138 562 4.03e-134 401
Msa0574620 MtrunA17_Chr3g0080391 46.813 455 212 8 103 546 139 574 3.99e-133 399
Msa0574620 MtrunA17_Chr7g0269511 43.396 530 276 8 35 546 41 564 2.05e-132 397
Msa0574620 MtrunA17_Chr1g0195331 43.063 555 283 11 18 546 8 555 4.43e-131 393
Msa0574620 MtrunA17_Chr4g0069881 42.308 520 263 12 36 546 44 535 6.19e-131 392
Msa0574620 MtrunA17_Chr8g0345211 42.857 532 253 13 36 546 81 582 1.01e-130 393
Msa0574620 MtrunA17_Chr4g0069861 43.478 483 223 7 85 546 64 517 1.10e-130 391
Msa0574620 MtrunA17_Chr1g0195301 42.703 555 285 11 18 546 8 555 1.13e-130 392
Msa0574620 MtrunA17_Chr1g0195321 42.703 555 285 11 18 546 8 555 1.13e-130 392
Msa0574620 MtrunA17_Chr1g0175661 42.105 551 287 10 21 546 15 558 7.92e-129 387
Msa0574620 MtrunA17_Chr8g0354911 40.942 552 270 12 35 546 77 612 9.94e-127 384
Msa0574620 MtrunA17_Chr1g0194091 39.854 547 291 11 27 546 34 569 2.19e-126 382
Msa0574620 MtrunA17_Chr1g0195351 42.342 555 285 12 18 546 8 553 1.08e-125 379
Msa0574620 MtrunA17_Chr1g0194081 38.878 499 285 10 63 546 70 563 1.27e-117 359
Msa0574620 MtrunA17_Chr1g0195311 60.920 261 101 1 286 546 55 314 3.06e-117 349
Msa0574620 MtrunA17_Chr3g0080381 38.559 555 281 13 35 546 91 628 9.04e-117 358
Msa0574620 MtrunA17_Chr1g0192311 38.697 522 288 12 36 542 62 566 4.35e-114 350
Msa0574620 MtrunA17_Chr1g0192281 38.697 522 288 12 36 542 62 566 4.35e-114 350
Msa0574620 MtrunA17_Chr1g0192301 39.425 487 266 10 72 542 98 571 3.51e-112 345
Msa0574620 MtrunA17_Chr1g0192321 37.500 544 285 14 36 545 60 582 4.31e-111 343
Msa0574620 MtrunA17_Chr7g0272151 39.364 503 264 11 72 545 92 582 1.98e-110 341
Msa0574620 MtrunA17_Chr4g0000121 39.220 487 267 10 72 542 80 553 3.19e-110 340
Msa0574620 MtrunA17_Chr4g0067611 39.238 525 288 12 36 545 63 571 2.05e-109 338
Msa0574620 MtrunA17_Chr4g0067601 39.245 530 287 14 33 545 60 571 8.16e-108 334
Msa0574620 MtrunA17_Chr7g0270661 39.511 491 265 9 79 541 83 569 5.95e-107 332
Msa0574620 MtrunA17_Chr7g0270671 39.511 491 265 9 79 541 83 569 5.95e-107 332
Msa0574620 MtrunA17_Chr2g0301931 53.169 284 128 3 263 546 14 292 4.42e-104 314
Msa0574620 MtrunA17_Chr7g0270651 38.247 502 278 9 73 546 77 574 2.28e-103 322
Msa0574620 MtrunA17_Chr2g0295531 35.606 528 293 12 33 542 44 542 3.04e-103 323
Msa0574620 MtrunA17_Chr7g0234711 61.404 228 87 1 320 546 42 269 3.05e-102 309
Msa0574620 MtrunA17_Chr2g0295551 37.453 534 286 12 33 545 57 563 2.10e-100 314
Msa0574620 MtrunA17_Chr7g0270691 36.875 480 262 9 88 546 90 549 7.24e-100 313
Msa0574620 MtrunA17_Chr2g0302001 53.150 254 118 1 293 546 185 437 8.93e-99 306
Msa0574620 MtrunA17_Chr7g0270631 38.604 487 267 9 79 537 71 553 4.94e-95 313
Msa0574620 MtrunA17_Chr7g0270631 37.967 482 264 10 79 529 619 1096 2.63e-84 283
Msa0574620 MtrunA17_Chr8g0391261 91.367 139 12 0 408 546 28 166 7.78e-91 276
Msa0574620 MtrunA17_Chr8g0384121 33.585 530 304 12 31 538 53 556 4.28e-90 288
Msa0574620 MtrunA17_Chr2g0295541 35.872 499 253 11 98 545 112 594 1.41e-89 287
Msa0574620 MtrunA17_Chr3g0129921 33.692 558 304 16 20 539 28 557 1.76e-89 286
Msa0574620 MtrunA17_Chr8g0380581 39.763 337 193 4 208 542 44 372 8.44e-85 268
Msa0574620 MtrunA17_Chr5g0437831 41.593 339 191 6 210 546 50 383 9.99e-84 265
Msa0574620 MtrunA17_Chr7g0268241 40.823 316 174 5 232 546 47 350 1.07e-77 248
Msa0574620 MtrunA17_Chr2g0334111 39.172 314 176 5 234 546 24 323 7.74e-77 245
Msa0574620 MtrunA17_Chr7g0271381 30.869 541 296 17 36 546 64 556 2.87e-68 230
Msa0574620 MtrunA17_Chr6g0487101 34.430 395 228 11 156 535 2 380 4.34e-53 184
Msa0574620 MtrunA17_Chr8g0389771 35.735 347 191 10 205 535 47 377 1.12e-50 178
Msa0574620 MtrunA17_Chr6g0453551 33.443 305 178 6 235 529 6 295 3.63e-49 172
Msa0574620 MtrunA17_Chr1g0194311 32.132 333 195 8 213 535 57 368 7.08e-49 173
Msa0574620 MtrunA17_Chr2g0299431 42.975 242 123 6 208 444 30 261 2.62e-48 169
Msa0574620 MtrunA17_Chr6g0466951 34.722 288 164 6 226 508 60 328 4.93e-48 170
Msa0574620 MtrunA17_Chr7g0258341 31.701 388 230 9 158 535 25 387 2.47e-47 169
Msa0574620 MtrunA17_Chr7g0217941 32.099 324 189 7 237 544 8 316 8.81e-47 166
Msa0574620 MtrunA17_Chr6g0466961 35.862 290 158 7 226 508 31 299 9.29e-47 166
Msa0574620 MtrunA17_Chr1g0161731 29.683 347 199 8 201 539 28 337 1.14e-42 155
Msa0574620 MtrunA17_Chr7g0234731 32.753 287 159 6 36 315 55 314 4.53e-41 150
Msa0574620 MtrunA17_Chr4g0070051 32.154 311 180 12 235 535 54 343 8.88e-38 142
Msa0574620 MtrunA17_Chr5g0446301 31.922 307 181 7 241 535 67 357 5.11e-37 140
Msa0574620 MtrunA17_Chr7g0265201 29.739 306 187 6 237 535 101 385 1.56e-36 140
Msa0574620 MtrunA17_Chr5g0440871 31.250 304 184 7 235 536 39 319 1.40e-35 135
Msa0574620 MtrunA17_Chr5g0399221 30.395 329 195 9 230 544 56 364 2.74e-35 135
Msa0574620 MtrunA17_Chr5g0437821 46.212 132 70 1 349 479 4 135 3.88e-35 128
Msa0574620 MtrunA17_Chr2g0294321 29.042 334 213 7 210 535 29 346 9.81e-34 131
Msa0574620 MtrunA17_Chr8g0351871 28.707 317 197 8 229 536 27 323 8.20e-32 125
Msa0574620 MtrunA17_Chr4g0037751 29.866 298 185 7 214 505 55 334 2.08e-31 125
Msa0574620 MtrunA17_Chr6g0459781 38.333 180 95 5 235 401 13 189 1.22e-30 119
Msa0574620 MtrunA17_Chr2g0295851 28.358 335 205 10 210 535 28 336 7.16e-30 120
Msa0574620 MtrunA17_Chr5g0420831 30.035 283 167 8 235 505 71 334 2.23e-29 119
Msa0574620 MtrunA17_Chr4g0025841 28.846 260 167 5 220 479 22 263 1.59e-25 106
Msa0574620 MtrunA17_Chr5g0440901 32.895 152 92 2 328 479 1 142 2.43e-22 93.6
Msa0574620 MtrunA17_Chr2g0314691 31.176 170 105 3 339 507 18 176 4.18e-20 89.0
Msa0574620 MtrunA17_Chr7g0265161 30.508 177 90 4 305 476 9 157 4.80e-20 89.7
Msa0574620 MtrunA17_Chr6g0460141 36.957 138 79 4 266 395 7 144 5.19e-19 84.0
Msa0574620 MtrunA17_Chr5g0440891 35.032 157 92 4 235 390 48 195 1.44e-18 84.3
Msa0574620 MtrunA17_Chr4g0073911 27.841 176 117 3 266 434 38 210 4.50e-16 78.2
Msa0574620 MtrunA17_Chr6g0459791 36.000 125 67 4 412 535 4 116 2.60e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574620 AT1G23200.1 53.846 533 210 12 35 546 37 554 0.0 554
Msa0574620 AT2G45220.1 49.027 514 223 11 36 546 34 511 7.34e-171 493
Msa0574620 AT3G49220.1 45.038 524 259 11 35 546 90 596 6.00e-149 441
Msa0574620 AT3G49220.3 45.038 524 259 11 35 546 135 641 2.07e-148 441
Msa0574620 AT5G53370.1 43.678 522 270 9 35 546 78 585 6.39e-147 435
Msa0574620 AT5G53370.2 43.678 522 270 9 35 546 114 621 2.47e-146 435
Msa0574620 AT3G60730.1 45.030 493 244 6 74 546 34 519 4.45e-146 431
Msa0574620 AT5G51490.1 41.825 526 264 9 36 546 38 536 2.51e-145 429
Msa0574620 AT5G51500.1 42.776 526 259 11 36 546 42 540 4.93e-144 426
Msa0574620 AT3G14310.1 42.518 548 262 9 35 546 62 592 1.10e-142 424
Msa0574620 AT3G47400.1 42.395 526 269 9 36 546 88 594 3.80e-142 423
Msa0574620 AT1G53830.1 42.778 540 262 9 36 546 66 587 9.03e-140 417
Msa0574620 AT1G11580.1 45.570 474 230 6 86 546 99 557 1.90e-139 415
Msa0574620 AT1G11580.2 45.570 474 230 6 86 546 120 578 3.86e-139 415
Msa0574620 AT3G59010.1 46.791 483 225 11 73 546 69 528 2.96e-138 411
Msa0574620 AT2G26440.1 43.238 525 266 10 36 546 41 547 3.99e-135 404
Msa0574620 AT4G00190.1 42.176 524 248 11 28 546 1 474 6.43e-134 398
Msa0574620 AT3G43270.1 45.492 488 240 7 73 546 50 525 1.65e-133 399
Msa0574620 AT4G02320.1 46.771 449 226 6 104 546 77 518 2.77e-133 398
Msa0574620 AT4G02330.1 41.739 575 277 14 18 546 9 571 2.83e-133 400
Msa0574620 AT2G26450.1 42.337 522 267 12 36 546 113 611 1.48e-132 399
Msa0574620 AT3G05620.1 43.522 494 233 9 73 546 74 541 4.72e-132 395
Msa0574620 AT4G02300.1 41.683 523 255 10 56 546 28 532 1.35e-131 394
Msa0574620 AT4G33220.1 38.559 555 284 8 16 546 2 523 2.81e-131 393
Msa0574620 AT2G43050.1 43.487 476 241 6 74 546 66 516 5.72e-131 392
Msa0574620 AT5G27870.1 41.379 522 275 12 36 546 61 562 1.17e-130 398
Msa0574620 AT5G04960.1 41.586 517 268 9 36 544 72 562 5.10e-130 391
Msa0574620 AT3G05610.1 40.494 526 276 14 36 546 62 565 3.12e-125 382
Msa0574620 AT3G10710.1 41.154 520 265 10 36 546 74 561 4.31e-125 378
Msa0574620 AT4G33220.2 45.185 405 207 4 147 546 8 402 1.13e-124 372
Msa0574620 AT1G02810.1 38.917 591 277 14 21 546 6 577 1.41e-123 375
Msa0574620 AT5G49180.1 40.228 527 282 13 33 542 56 566 2.53e-123 374
Msa0574620 AT2G47550.1 44.792 480 235 10 92 546 84 558 6.51e-122 370
Msa0574620 AT3G10720.2 39.042 543 291 7 35 546 82 615 1.07e-121 371
Msa0574620 AT4G33230.1 40.153 523 286 11 36 546 99 606 3.01e-121 370
Msa0574620 AT3G06830.1 40.351 513 278 12 51 544 63 566 3.07e-121 369
Msa0574620 AT5G04970.1 40.037 547 282 10 34 546 86 620 9.90e-121 369
Msa0574620 AT1G53840.1 38.606 531 274 14 36 540 77 581 1.38e-118 362
Msa0574620 AT5G20860.2 41.538 455 226 9 106 546 110 538 3.06e-117 357
Msa0574620 AT3G14300.1 39.544 526 276 13 36 541 463 966 1.58e-115 365
Msa0574620 AT3G10720.1 56.154 260 113 1 287 546 1 259 3.57e-107 322
Msa0574620 AT4G03930.1 36.314 548 296 12 20 546 9 524 9.93e-105 325
Msa0574620 AT4G15980.1 47.246 345 178 4 201 544 359 700 9.73e-104 327
Msa0574620 AT4G15980.2 47.246 345 178 4 201 544 369 710 1.69e-103 327
Msa0574620 AT3G27980.1 47.478 337 169 3 210 546 169 497 1.06e-102 318
Msa0574620 AT1G11590.1 40.045 447 238 7 110 546 98 524 6.93e-102 317
Msa0574620 AT5G20860.1 38.444 437 201 8 103 525 107 489 1.59e-96 303
Msa0574620 AT5G09760.1 33.955 536 295 12 33 539 46 551 6.01e-92 292
Msa0574620 AT5G09760.2 33.955 536 295 12 33 539 72 577 1.15e-91 292
Msa0574620 AT1G11370.1 50.000 258 128 1 221 478 32 288 1.52e-87 272
Msa0574620 AT2G47030.1 42.368 380 194 8 185 546 216 588 4.02e-87 281
Msa0574620 AT2G47040.1 43.501 377 191 9 185 546 226 595 6.50e-87 280
Msa0574620 AT5G64640.1 33.333 525 310 13 33 536 94 599 6.58e-87 280
Msa0574620 AT3G62170.1 37.395 476 254 13 104 546 124 588 3.64e-85 276
Msa0574620 AT5G19730.1 36.278 317 174 8 231 535 81 381 1.13e-51 181
Msa0574620 AT3G29090.1 34.507 284 164 4 235 508 7 278 4.83e-49 172
Msa0574620 AT1G05310.1 34.259 324 184 7 225 535 81 388 7.95e-48 171
Msa0574620 AT2G36710.1 33.766 308 184 5 235 535 91 385 4.29e-45 164
Msa0574620 AT2G36700.1 31.579 304 189 5 237 535 44 333 3.83e-44 159
Msa0574620 AT1G44980.1 44.211 190 104 2 210 399 54 241 1.11e-41 150
Msa0574620 AT3G17060.1 30.000 310 189 7 235 535 46 336 4.15e-40 149
Msa0574620 AT2G21610.1 30.464 302 189 6 235 535 51 332 7.89e-40 148
Msa0574620 AT2G21610.2 30.464 302 189 6 235 535 56 337 1.43e-39 148
Msa0574620 AT3G24130.1 31.818 286 170 6 234 508 33 304 2.66e-39 146
Msa0574620 AT5G47500.1 30.909 330 194 9 229 544 53 362 1.21e-37 142
Msa0574620 AT5G18990.1 32.000 300 172 7 220 508 21 299 1.68e-37 141
Msa0574620 AT5G07430.1 30.492 305 192 6 235 535 67 355 1.06e-36 140
Msa0574620 AT5G55590.1 33.758 314 178 9 235 535 82 378 4.57e-35 136
Msa0574620 AT3G49220.2 33.333 294 167 11 35 316 135 411 2.35e-34 135
Msa0574620 AT5G26810.1 30.159 315 193 9 235 536 34 334 2.91e-34 132
Msa0574620 AT5G55590.2 33.852 257 145 7 235 479 82 325 2.46e-33 130
Msa0574620 AT5G61680.1 29.085 306 196 7 235 535 69 358 2.58e-33 130
Msa0574620 AT2G19150.1 28.659 328 203 8 226 542 32 339 5.67e-33 129
Msa0574620 AT5G07420.1 29.450 309 190 10 235 535 67 355 4.63e-31 124
Msa0574620 AT2G19150.2 29.658 263 161 6 226 479 32 279 1.19e-28 115
Msa0574620 AT2G47280.1 28.296 311 197 7 235 536 35 328 1.55e-28 116
Msa0574620 AT1G69940.1 26.510 298 198 7 243 535 74 355 1.30e-27 114
Msa0574620 AT5G07410.1 26.174 298 199 7 243 535 74 355 1.43e-26 111

Find 168 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCATATTGGCTTTC+AGG 0.136783 4_1:-78225592 Msa0574620:CDS
GCTGGTGATTCTTGGATTTC+AGG 0.138272 4_1:-78224140 Msa0574620:CDS
GTGACAAATTTAATGATATT+TGG 0.156463 4_1:-78225367 Msa0574620:CDS
CACAAATTAGTATACCTTTA+TGG 0.175493 4_1:-78226388 Msa0574620:five_prime_UTR
GCCAAGTTAATTTGTCATTT+AGG 0.213459 4_1:+78225833 None:intergenic
AATAATTATTGCAGCTGTTA+TGG 0.232312 4_1:-78224792 Msa0574620:intron
CTAACATTTCACAAAGTTCT+TGG 0.238259 4_1:+78226468 None:intergenic
CTTCCATGATATTGCACTTA+AGG 0.261043 4_1:-78226010 Msa0574620:CDS
GCATTGAGTACACTTTATTA+TGG 0.267204 4_1:-78224278 Msa0574620:CDS
AATCATCAAACTTGTCAAAA+TGG 0.271720 4_1:-78225793 Msa0574620:CDS
ACAATCTTCCCATGCTGATT+TGG 0.281247 4_1:+78225912 None:intergenic
CAGATAATAAATTTCCATAT+TGG 0.306714 4_1:-78225600 Msa0574620:CDS
TCTGACCCTGAAAGCCAATA+TGG 0.325074 4_1:+78225586 None:intergenic
TAATTTGTGGGTGTAAGTTT+AGG 0.327047 4_1:+78226402 None:intergenic
TCTATGTATATTTGACCCTT+TGG 0.330671 4_1:+78223875 None:intergenic
AAGATGATGAAGAAGAATCT+AGG 0.330716 4_1:+78226040 None:intergenic
CTGGTGATTCTTGGATTTCA+GGG 0.333928 4_1:-78224139 Msa0574620:CDS
AATCATTTGATGCGGTTATA+CGG 0.335152 4_1:+78224435 None:intergenic
TTGTGGTGGTTCCAATATAA+TGG 0.354156 4_1:+78226076 None:intergenic
ATAATTATTGCAGCTGTTAT+GGG 0.354501 4_1:-78224791 Msa0574620:intron
TCATCAAGTACTAAACAAAA+TGG 0.356786 4_1:-78225640 Msa0574620:CDS
TTGGCTTTGGCGGGCCATAA+AGG 0.377975 4_1:+78226374 None:intergenic
CTTCAGGCAACACCGGGGTC+AGG 0.382501 4_1:-78225553 Msa0574620:CDS
TAATTGAAGTCTATAAAGAT+AGG 0.382638 4_1:-78223986 Msa0574620:three_prime_UTR
AATTTCCATATTGGCTTTCA+GGG 0.383402 4_1:-78225591 Msa0574620:CDS
AATGGTCATATTGTATTGTA+TGG 0.386077 4_1:+78226432 None:intergenic
GGAACAATTGATTTCATCTT+CGG 0.386944 4_1:-78224587 Msa0574620:CDS
TATATATAGATCAAGAGATT+TGG 0.395187 4_1:+78226355 None:intergenic
GGTAATCAAAATGCTATAGA+TGG 0.400131 4_1:-78225316 Msa0574620:CDS
TTTGATAAATTCAGAAGGTT+GGG 0.405145 4_1:-78224321 Msa0574620:CDS
CATGTGTGCAACCATTATAT+TGG 0.409559 4_1:-78226087 Msa0574620:CDS
TCCATTTCCTTTACTCCAAA+GGG 0.409561 4_1:-78223890 Msa0574620:three_prime_UTR
ACATTTCGTTCAGCTACTTT+CGG 0.410470 4_1:-78225286 Msa0574620:intron
ACCCTTTGGAGTAAAGGAAA+TGG 0.411609 4_1:+78223889 None:intergenic
ATACTGGTGGAAGAGTTAAT+TGG 0.417090 4_1:-78224226 Msa0574620:CDS
GTAATGAAAAGTAATATTGA+TGG 0.420796 4_1:-78224344 Msa0574620:CDS
GTCCATTTCCTTTACTCCAA+AGG 0.421976 4_1:-78223891 Msa0574620:three_prime_UTR
TATTTGACCCTTTGGAGTAA+AGG 0.423968 4_1:+78223883 None:intergenic
TAGGGTTGATAGTTTATTTG+TGG 0.428236 4_1:+78226059 None:intergenic
GCTGCTAAGCTTAGTGGAAA+AGG 0.429873 4_1:-78225451 Msa0574620:CDS
CGCATCAAATGATTTGAAAC+CGG 0.431620 4_1:-78224426 Msa0574620:CDS
TTCACTCTCTTGTTTGTGCA+TGG 0.431690 4_1:-78226144 Msa0574620:CDS
TGTTCTTCCCTGTGCTGTTA+CGG 0.432458 4_1:+78224496 None:intergenic
ATGGGAAGAGCTAGGTGTAT+AGG 0.434996 4_1:+78226268 None:intergenic
GTTGGTGGTGGTGCTAATAC+TGG 0.437055 4_1:-78224242 Msa0574620:CDS
AATTCAGTTGAAACTTATCT+TGG 0.438603 4_1:-78224398 Msa0574620:CDS
CAAGCAGTAGCTCTCCGTTC+CGG 0.442034 4_1:-78224710 Msa0574620:CDS
GTCAGATAGAAAGCTTCTTC+AGG 0.443922 4_1:-78225569 Msa0574620:CDS
GTTTGATAAATTCAGAAGGT+TGG 0.445073 4_1:-78224322 Msa0574620:CDS
ATCAAGAGATTTGGCTTTGG+CGG 0.445220 4_1:+78226364 None:intergenic
GTTTCAACTGAATTCTGAAC+CGG 0.449894 4_1:+78224407 None:intergenic
TTTGAAATTATTTAATTCCA+TGG 0.450557 4_1:+78225945 None:intergenic
GTGTTCTGTTGGTTGCTCAT+TGG 0.453378 4_1:+78224518 None:intergenic
GCAACACCGGGGTCAGGGAC+AGG 0.454024 4_1:-78225547 Msa0574620:CDS
AAGAGAATGTTGATATCAAA+AGG 0.455565 4_1:-78225393 Msa0574620:CDS
GGTGTCTTGGTAGCCTTTGA+AGG 0.460911 4_1:+78224658 None:intergenic
TTAAGTAGTAATAATAAAAG+AGG 0.461839 4_1:+78223769 None:intergenic
TAGATCAAGAGATTTGGCTT+TGG 0.463035 4_1:+78226361 None:intergenic
GTGAAGTTGCTTAACATGGA+TGG 0.463236 4_1:+78225730 None:intergenic
GTCATATTGTATTGTATGGA+AGG 0.464123 4_1:+78226436 None:intergenic
ATTCAGAAGGTTGGGCTCCA+TGG 0.464333 4_1:-78224313 Msa0574620:CDS
GTTTGTGTGCTTCAATTGCT+TGG 0.467097 4_1:+78225977 None:intergenic
ATTGCAGCTGTTATGGGAGA+TGG 0.472604 4_1:-78224785 Msa0574620:intron
TGAGATGAGATGATGTATAG+TGG 0.472882 4_1:-78224051 Msa0574620:three_prime_UTR
TAACCTTAAGTGCAATATCA+TGG 0.473824 4_1:+78226007 None:intergenic
AGATGATGAAGAAGAATCTA+GGG 0.475990 4_1:+78226041 None:intergenic
GGACATGACCTTTGAGAATA+CGG 0.477411 4_1:-78224750 Msa0574620:CDS
AATGATATTTGGAGATGGAA+TGG 0.477734 4_1:-78225356 Msa0574620:CDS
GAAGGTTGGGCTCCATGGAT+GGG 0.479662 4_1:-78224308 Msa0574620:CDS
AGAAGGTTGGGCTCCATGGA+TGG 0.481727 4_1:-78224309 Msa0574620:CDS
GATGCAGCTGCTAAGCTTAG+TGG 0.483722 4_1:-78225457 Msa0574620:CDS
ACAAGTTTGATGATTGGTAA+TGG 0.484728 4_1:+78225801 None:intergenic
GTTGTTATTCATCTGAAAGC+AGG 0.486478 4_1:-78225424 Msa0574620:CDS
CATAAAGGTATACTAATTTG+TGG 0.487591 4_1:+78226389 None:intergenic
TGCAGTCGCGGTAGAATTGA+CGG 0.487904 4_1:+78224618 None:intergenic
TTCTACCGTTGTGCCTTCAA+AGG 0.490213 4_1:-78224671 Msa0574620:CDS
GTAAGTTTAGGATGAAGAAA+TGG 0.494613 4_1:+78226414 None:intergenic
AATAATAAATGGGAAGAGCT+AGG 0.499818 4_1:+78226260 None:intergenic
ACAGAACACCGTAACAGCAC+AGG 0.500933 4_1:-78224504 Msa0574620:CDS
GGGAAGTAGTTTAAGTGAGA+GGG 0.501534 4_1:+78225751 None:intergenic
GGTTGATAGTTTATTTGTGG+TGG 0.502880 4_1:+78226062 None:intergenic
AGATTGTTTAGAGCTTTATG+AGG 0.504717 4_1:-78225896 Msa0574620:CDS
CTGCTAAGCTTAGTGGAAAA+GGG 0.504769 4_1:-78225450 Msa0574620:CDS
AGTGGTGTGCCGTTTGATGC+AGG 0.508356 4_1:-78224116 Msa0574620:CDS
ACCTAAATGACAAATTAACT+TGG 0.508748 4_1:-78225834 Msa0574620:CDS
AAGCTTCTTCAGGCAACACC+GGG 0.510144 4_1:-78225559 Msa0574620:CDS
TCAAGAGATTTGGCTTTGGC+GGG 0.510356 4_1:+78226365 None:intergenic
TGAAGTTGCTTAACATGGAT+GGG 0.511299 4_1:+78225731 None:intergenic
TGCTTATGAAATTAAGTGCA+AGG 0.515674 4_1:+78226323 None:intergenic
TGAAATTAAGTGCAAGGGAA+TGG 0.518172 4_1:+78226329 None:intergenic
GTTCTTGAATATTTCTGCCA+TGG 0.518439 4_1:+78224371 None:intergenic
GGTGGAAGAGTTAATTGGCC+TGG 0.520232 4_1:-78224221 Msa0574620:CDS
TAAGACAATATCTGAGGGTG+TGG 0.520252 4_1:-78225479 Msa0574620:CDS
GGGAGATGGTTTCATAGCTA+AGG 0.523196 4_1:-78224771 Msa0574620:CDS
GAAGGCACAACGGTAGAAAA+CGG 0.523739 4_1:+78224676 None:intergenic
TGGTTGCACACATGAGGGTA+TGG 0.524847 4_1:+78226096 None:intergenic
CTTGGTCCTGTCCCTGACCC+CGG 0.525603 4_1:+78225541 None:intergenic
ATTCACAAACCTGCATCAAA+CGG 0.526344 4_1:+78224107 None:intergenic
TATTGCACTTAAGGTTACCT+TGG 0.526896 4_1:-78226001 Msa0574620:CDS
TTTAGTGAAGTTGCTTAACA+TGG 0.527598 4_1:+78225726 None:intergenic
TACCGCGACTGCAACATCTA+CGG 0.528232 4_1:-78224608 Msa0574620:CDS
CATAAGCATACAAGGTGTCT+TGG 0.531685 4_1:+78224645 None:intergenic
TTCTTGGTCGTGTTATGTGA+TGG 0.532587 4_1:+78226484 None:intergenic
AAGACTTGAAATGATGACTT+TGG 0.535176 4_1:-78224026 Msa0574620:three_prime_UTR
TGAAGTTAGAGTGCTGCTAA+TGG 0.537879 4_1:+78225684 None:intergenic
TGTTATTACTAATCCTTCTG+AGG 0.537907 4_1:-78224192 Msa0574620:CDS
GTGCTGTTACGGTGTTCTGT+TGG 0.539536 4_1:+78224507 None:intergenic
GGTTGGGCTCCATGGATGGG+TGG 0.540114 4_1:-78224305 Msa0574620:CDS
AATTTAATGATATTTGGAGA+TGG 0.540497 4_1:-78225361 Msa0574620:CDS
TATGGTGAATATATGAATGT+TGG 0.547151 4_1:-78224260 Msa0574620:CDS
ATGAAATAGTGTGCATGTTG+TGG 0.550100 4_1:+78226220 None:intergenic
AAAGCTTCTTCAGGCAACAC+CGG 0.552192 4_1:-78225560 Msa0574620:CDS
GAAAATTTAACAGCCTCAGA+AGG 0.553132 4_1:+78224179 None:intergenic
AATTGAAGTCTATAAAGATA+GGG 0.553959 4_1:-78223985 Msa0574620:three_prime_UTR
TTCCGTAGATGTTGCAGTCG+CGG 0.554832 4_1:+78224606 None:intergenic
GTGGTTGCACAAGATGGAAG+TGG 0.556036 4_1:-78225508 Msa0574620:CDS
TGGGAAGTAGTTTAAGTGAG+AGG 0.556743 4_1:+78225750 None:intergenic
GCTTATGAAATTAAGTGCAA+GGG 0.558485 4_1:+78226324 None:intergenic
CTTAAGTGCAATATCATGGA+AGG 0.559044 4_1:+78226011 None:intergenic
GATTCTTGGATTTCAGGGAG+TGG 0.559486 4_1:-78224134 Msa0574620:CDS
CGGAGTGATCGGCGCCGGAA+CGG 0.559791 4_1:+78224696 None:intergenic
ATAAAGGTATACTAATTTGT+GGG 0.563799 4_1:+78226390 None:intergenic
AGTAGTTTAAGTGAGAGGGA+AGG 0.567101 4_1:+78225755 None:intergenic
TGTTATGTGATGGGCCATGC+AGG 0.567785 4_1:+78226494 None:intergenic
ACGGTAGAAAACGGAGTGAT+CGG 0.568769 4_1:+78224685 None:intergenic
GGGTCCTGCCGTATTCTCAA+AGG 0.569689 4_1:+78224742 None:intergenic
CAGAACACCGTAACAGCACA+GGG 0.570741 4_1:-78224503 Msa0574620:CDS
ATGGATTCTACTATTGTCAC+TGG 0.572496 4_1:-78225337 Msa0574620:CDS
TCTTGGTCGTGTTATGTGAT+GGG 0.573595 4_1:+78226485 None:intergenic
ATATAATGGTTGCACACATG+AGG 0.574017 4_1:+78226090 None:intergenic
CGGAACGGAGAGCTACTGCT+TGG 0.579824 4_1:+78224711 None:intergenic
GATGGTTTGATAAATTCAGA+AGG 0.580230 4_1:-78224326 Msa0574620:CDS
AATGCAAAACCACCCATCCA+TGG 0.580980 4_1:+78224296 None:intergenic
GAAAACGGAGTGATCGGCGC+CGG 0.581382 4_1:+78224691 None:intergenic
TGCTTCAATTGCTTGGTCCA+AGG 0.583595 4_1:+78225984 None:intergenic
CGGTTTCAAATCATTTGATG+CGG 0.588448 4_1:+78224427 None:intergenic
TCAAGTACTAAACAAAATGG+AGG 0.588650 4_1:-78225637 Msa0574620:CDS
GGTGAATATATGAATGTTGG+TGG 0.591996 4_1:-78224257 Msa0574620:CDS
GATATCGTGGTTGCACAAGA+TGG 0.592172 4_1:-78225514 Msa0574620:CDS
ATGACCTTTGAGAATACGGC+AGG 0.599978 4_1:-78224746 Msa0574620:CDS
TTCAGGCAACACCGGGGTCA+GGG 0.601142 4_1:-78225552 Msa0574620:CDS
GATGATGTAGATGCGGATGA+TGG 0.603143 4_1:+78225658 None:intergenic
ACGGTTGGCATAAGCATACA+AGG 0.605300 4_1:+78224637 None:intergenic
GAACTATAAGACAATATCTG+AGG 0.605352 4_1:-78225485 Msa0574620:CDS
TATAATGGTTGCACACATGA+GGG 0.607421 4_1:+78226091 None:intergenic
GAAAGTAGCTGAACGAAATG+TGG 0.611952 4_1:+78225288 None:intergenic
AACTATAAGACAATATCTGA+GGG 0.612206 4_1:-78225484 Msa0574620:CDS
AAACTTATCTTGGTAGACCA+TGG 0.615078 4_1:-78224388 Msa0574620:CDS
TAAGCATGCCAAATCAGCAT+GGG 0.616130 4_1:-78225920 Msa0574620:CDS
CTAAACAAAATGGAGGGAGA+AGG 0.620123 4_1:-78225630 Msa0574620:CDS
GAATATATGAATGTTGGTGG+TGG 0.621736 4_1:-78224254 Msa0574620:CDS
GTCGCGGTAGAATTGACGGT+TGG 0.627744 4_1:+78224622 None:intergenic
CAAGTACTAAACAAAATGGA+GGG 0.628274 4_1:-78225636 Msa0574620:CDS
AGGACCAAGTGCTGATATCG+TGG 0.631260 4_1:-78225527 Msa0574620:CDS
ATAAGCATGCCAAATCAGCA+TGG 0.635295 4_1:-78225921 Msa0574620:CDS
ACAGATCCAAATGAAAACAC+AGG 0.641536 4_1:-78224476 Msa0574620:CDS
TGGTTGCACAAGATGGAAGT+GGG 0.642324 4_1:-78225507 Msa0574620:CDS
TTTCACGTAAACTTCCTGCA+TGG 0.652686 4_1:-78226508 Msa0574620:five_prime_UTR
GCAACCACGATATCAGCACT+TGG 0.658862 4_1:+78225523 None:intergenic
GGTGGTGGTGCTAATACTGG+TGG 0.661832 4_1:-78224239 Msa0574620:CDS
AGTACTTGATGATGTAGATG+CGG 0.671746 4_1:+78225651 None:intergenic
ATGGCAGAAATATTCAAGAA+CGG 0.671890 4_1:-78224369 Msa0574620:CDS
TTAGTAATAACATGAAAACC+AGG 0.674041 4_1:+78224203 None:intergenic
TGTATAGGAATGAATACGAG+AGG 0.675433 4_1:+78226283 None:intergenic
ACACAAACTTGTATCAACCA+TGG 0.676347 4_1:-78225962 Msa0574620:CDS
GGTAGCCTTTGAAGGCACAA+CGG 0.688392 4_1:+78224666 None:intergenic
AGCTTCTTCAGGCAACACCG+GGG 0.762405 4_1:-78225558 Msa0574620:CDS
AAGTTTACGTGAAATATACG+AGG 0.765155 4_1:+78226517 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTCTATAATAATAAAT+GGG + chr4_1:78224053-78224072 None:intergenic 10.0%
!!! TGTTTTTCTATAATAATAAA+TGG + chr4_1:78224054-78224073 None:intergenic 10.0%
!!! TTTGAAATTATTTAATTCCA+TGG + chr4_1:78224358-78224377 None:intergenic 15.0%
!! CAAAAAATATATATGATCAA+TGG - chr4_1:78225141-78225160 Msa0574620:intron 15.0%
!!! ATAAAGGTATACTAATTTGT+GGG + chr4_1:78223913-78223932 None:intergenic 20.0%
!! TATATATAGATCAAGAGATT+TGG + chr4_1:78223948-78223967 None:intergenic 20.0%
!!! CACTATTTCATTTTCTAATA+TGG - chr4_1:78224092-78224111 Msa0574620:exon 20.0%
!! CAGATAATAAATTTCCATAT+TGG - chr4_1:78224700-78224719 Msa0574620:CDS 20.0%
!! GTGACAAATTTAATGATATT+TGG - chr4_1:78224933-78224952 Msa0574620:intron 20.0%
!! AATTTAATGATATTTGGAGA+TGG - chr4_1:78224939-78224958 Msa0574620:intron 20.0%
!!! AATAGATATTGGTTGATTAA+TGG + chr4_1:78225053-78225072 None:intergenic 20.0%
!!! TTATCTGTTTTGAGAAATAA+AGG + chr4_1:78225394-78225413 None:intergenic 20.0%
!!! AAACTACTCTCTTTTTTATT+TGG - chr4_1:78225423-78225442 Msa0574620:CDS 20.0%
!!! AACTACTCTCTTTTTTATTT+GGG - chr4_1:78225424-78225443 Msa0574620:CDS 20.0%
!! GTAATGAAAAGTAATATTGA+TGG - chr4_1:78225956-78225975 Msa0574620:CDS 20.0%
!!! ATTTTCTGTTGGTAATTTTT+TGG - chr4_1:78226138-78226157 Msa0574620:CDS 20.0%
!! TAATTGAAGTCTATAAAGAT+AGG - chr4_1:78226314-78226333 Msa0574620:five_prime_UTR 20.0%
!! AATTGAAGTCTATAAAGATA+GGG - chr4_1:78226315-78226334 Msa0574620:five_prime_UTR 20.0%
! AATGGTCATATTGTATTGTA+TGG + chr4_1:78223871-78223890 None:intergenic 25.0%
!! CATAAAGGTATACTAATTTG+TGG + chr4_1:78223914-78223933 None:intergenic 25.0%
! CACAAATTAGTATACCTTTA+TGG - chr4_1:78223912-78223931 Msa0574620:three_prime_UTR 25.0%
! ACCTAAATGACAAATTAACT+TGG - chr4_1:78224466-78224485 Msa0574620:CDS 25.0%
!!! ATTTTGACAAGTTTGATGAT+TGG + chr4_1:78224508-78224527 None:intergenic 25.0%
! AATCATCAAACTTGTCAAAA+TGG - chr4_1:78224507-78224526 Msa0574620:CDS 25.0%
! TCATCAAGTACTAAACAAAA+TGG - chr4_1:78224660-78224679 Msa0574620:CDS 25.0%
! AACTATAAGACAATATCTGA+GGG - chr4_1:78224816-78224835 Msa0574620:intron 25.0%
! AAGAGAATGTTGATATCAAA+AGG - chr4_1:78224907-78224926 Msa0574620:intron 25.0%
!!! TTTTGTTGTTTCTATATGCA+TGG - chr4_1:78225115-78225134 Msa0574620:intron 25.0%
! AATAATTATTGCAGCTGTTA+TGG - chr4_1:78225508-78225527 Msa0574620:CDS 25.0%
! ATAATTATTGCAGCTGTTAT+GGG - chr4_1:78225509-78225528 Msa0574620:CDS 25.0%
! AATTCAGTTGAAACTTATCT+TGG - chr4_1:78225902-78225921 Msa0574620:CDS 25.0%
! TTTGATAAATTCAGAAGGTT+GGG - chr4_1:78225979-78225998 Msa0574620:CDS 25.0%
! TATGGTGAATATATGAATGT+TGG - chr4_1:78226040-78226059 Msa0574620:CDS 25.0%
! TTAGTAATAACATGAAAACC+AGG + chr4_1:78226100-78226119 None:intergenic 25.0%
!! TATTGTCATGTAGTTTTTGT+TGG - chr4_1:78226342-78226361 Msa0574620:five_prime_UTR 25.0%
!! ATTGTCATGTAGTTTTTGTT+GGG - chr4_1:78226343-78226362 Msa0574620:five_prime_UTR 25.0%
!! TCTTGTATAACTTTTGTACA+AGG + chr4_1:78226374-78226393 None:intergenic 25.0%
CTAACATTTCACAAAGTTCT+TGG + chr4_1:78223835-78223854 None:intergenic 30.0%
GTCATATTGTATTGTATGGA+AGG + chr4_1:78223867-78223886 None:intergenic 30.0%
! GTAAGTTTAGGATGAAGAAA+TGG + chr4_1:78223889-78223908 None:intergenic 30.0%
! TAATTTGTGGGTGTAAGTTT+AGG + chr4_1:78223901-78223920 None:intergenic 30.0%
GCTTATGAAATTAAGTGCAA+GGG + chr4_1:78223979-78223998 None:intergenic 30.0%
TGCTTATGAAATTAAGTGCA+AGG + chr4_1:78223980-78223999 None:intergenic 30.0%
AATAATAAATGGGAAGAGCT+AGG + chr4_1:78224043-78224062 None:intergenic 30.0%
!! TAGGGTTGATAGTTTATTTG+TGG + chr4_1:78224244-78224263 None:intergenic 30.0%
AGATGATGAAGAAGAATCTA+GGG + chr4_1:78224262-78224281 None:intergenic 30.0%
AAGATGATGAAGAAGAATCT+AGG + chr4_1:78224263-78224282 None:intergenic 30.0%
TAACCTTAAGTGCAATATCA+TGG + chr4_1:78224296-78224315 None:intergenic 30.0%
! AGATTGTTTAGAGCTTTATG+AGG - chr4_1:78224404-78224423 Msa0574620:CDS 30.0%
!! GCCAAGTTAATTTGTCATTT+AGG + chr4_1:78224470-78224489 None:intergenic 30.0%
! ACAAGTTTGATGATTGGTAA+TGG + chr4_1:78224502-78224521 None:intergenic 30.0%
TTTAGTGAAGTTGCTTAACA+TGG + chr4_1:78224577-78224596 None:intergenic 30.0%
TCAAGTACTAAACAAAATGG+AGG - chr4_1:78224663-78224682 Msa0574620:CDS 30.0%
CAAGTACTAAACAAAATGGA+GGG - chr4_1:78224664-78224683 Msa0574620:CDS 30.0%
!! AAATTTCCATATTGGCTTTC+AGG - chr4_1:78224708-78224727 Msa0574620:CDS 30.0%
!! AATTTCCATATTGGCTTTCA+GGG - chr4_1:78224709-78224728 Msa0574620:CDS 30.0%
GAACTATAAGACAATATCTG+AGG - chr4_1:78224815-78224834 Msa0574620:intron 30.0%
AATGATATTTGGAGATGGAA+TGG - chr4_1:78224944-78224963 Msa0574620:intron 30.0%
GGTAATCAAAATGCTATAGA+TGG - chr4_1:78224984-78225003 Msa0574620:intron 30.0%
AGAGCTACATGAATAGATAT+TGG + chr4_1:78225064-78225083 None:intergenic 30.0%
! GTTTTGTACAAACTGTGTTA+CGG - chr4_1:78225254-78225273 Msa0574620:intron 30.0%
ATGAGTTGCTAAGTAAAGTA+TGG + chr4_1:78225451-78225470 None:intergenic 30.0%
ATACTTTACTTAGCAACTCA+TGG - chr4_1:78225450-78225469 Msa0574620:CDS 30.0%
! GGAACAATTGATTTCATCTT+CGG - chr4_1:78225713-78225732 Msa0574620:CDS 30.0%
! ACAATTCCTGTGTTTTCATT+TGG + chr4_1:78225833-78225852 None:intergenic 30.0%
AATCATTTGATGCGGTTATA+CGG + chr4_1:78225868-78225887 None:intergenic 30.0%
ATGGCAGAAATATTCAAGAA+CGG - chr4_1:78225931-78225950 Msa0574620:CDS 30.0%
GATGGTTTGATAAATTCAGA+AGG - chr4_1:78225974-78225993 Msa0574620:CDS 30.0%
GTTTGATAAATTCAGAAGGT+TGG - chr4_1:78225978-78225997 Msa0574620:CDS 30.0%
GCATTGAGTACACTTTATTA+TGG - chr4_1:78226022-78226041 Msa0574620:CDS 30.0%
TGTTATTACTAATCCTTCTG+AGG - chr4_1:78226108-78226127 Msa0574620:CDS 30.0%
AAGACTTGAAATGATGACTT+TGG - chr4_1:78226274-78226293 Msa0574620:five_prime_UTR 30.0%
TCTATGTATATTTGACCCTT+TGG + chr4_1:78226428-78226447 None:intergenic 30.0%
! TAGATCAAGAGATTTGGCTT+TGG + chr4_1:78223942-78223961 None:intergenic 35.0%
TGAAATTAAGTGCAAGGGAA+TGG + chr4_1:78223974-78223993 None:intergenic 35.0%
TGTATAGGAATGAATACGAG+AGG + chr4_1:78224020-78224039 None:intergenic 35.0%
ATGAAATAGTGTGCATGTTG+TGG + chr4_1:78224083-78224102 None:intergenic 35.0%
TATAATGGTTGCACACATGA+GGG + chr4_1:78224212-78224231 None:intergenic 35.0%
ATATAATGGTTGCACACATG+AGG + chr4_1:78224213-78224232 None:intergenic 35.0%
CATGTGTGCAACCATTATAT+TGG - chr4_1:78224213-78224232 Msa0574620:CDS 35.0%
TTGTGGTGGTTCCAATATAA+TGG + chr4_1:78224227-78224246 None:intergenic 35.0%
!! GGTTGATAGTTTATTTGTGG+TGG + chr4_1:78224241-78224260 None:intergenic 35.0%
CTTAAGTGCAATATCATGGA+AGG + chr4_1:78224292-78224311 None:intergenic 35.0%
CTTCCATGATATTGCACTTA+AGG - chr4_1:78224290-78224309 Msa0574620:CDS 35.0%
TATTGCACTTAAGGTTACCT+TGG - chr4_1:78224299-78224318 Msa0574620:CDS 35.0%
ACACAAACTTGTATCAACCA+TGG - chr4_1:78224338-78224357 Msa0574620:CDS 35.0%
TGAAGTTGCTTAACATGGAT+GGG + chr4_1:78224572-78224591 None:intergenic 35.0%
!! AGTACTTGATGATGTAGATG+CGG + chr4_1:78224652-78224671 None:intergenic 35.0%
! GTTGTTATTCATCTGAAAGC+AGG - chr4_1:78224876-78224895 Msa0574620:intron 35.0%
ATGGATTCTACTATTGTCAC+TGG - chr4_1:78224963-78224982 Msa0574620:intron 35.0%
! ACATTTCGTTCAGCTACTTT+CGG - chr4_1:78225014-78225033 Msa0574620:intron 35.0%
! ACTTTTGCCCCATTAAGATA+GGG - chr4_1:78225217-78225236 Msa0574620:intron 35.0%
ACAGATCCAAATGAAAACAC+AGG - chr4_1:78225824-78225843 Msa0574620:CDS 35.0%
CGGTTTCAAATCATTTGATG+CGG + chr4_1:78225876-78225895 None:intergenic 35.0%
! CGCATCAAATGATTTGAAAC+CGG - chr4_1:78225874-78225893 Msa0574620:CDS 35.0%
GTTTCAACTGAATTCTGAAC+CGG + chr4_1:78225896-78225915 None:intergenic 35.0%
AAACTTATCTTGGTAGACCA+TGG - chr4_1:78225912-78225931 Msa0574620:CDS 35.0%
! GTTCTTGAATATTTCTGCCA+TGG + chr4_1:78225932-78225951 None:intergenic 35.0%
GGTGAATATATGAATGTTGG+TGG - chr4_1:78226043-78226062 Msa0574620:CDS 35.0%
! GAATATATGAATGTTGGTGG+TGG - chr4_1:78226046-78226065 Msa0574620:CDS 35.0%
ATACTGGTGGAAGAGTTAAT+TGG - chr4_1:78226074-78226093 Msa0574620:CDS 35.0%
GAAAATTTAACAGCCTCAGA+AGG + chr4_1:78226124-78226143 None:intergenic 35.0%
! GAGGCTGTTAAATTTTCTGT+TGG - chr4_1:78226127-78226146 Msa0574620:CDS 35.0%
!!! TCTGTTGGTAATTTTTTGGC+TGG - chr4_1:78226142-78226161 Msa0574620:CDS 35.0%
!!! ATTTTTTGGCTGGTGATTCT+TGG - chr4_1:78226152-78226171 Msa0574620:CDS 35.0%
ATTCACAAACCTGCATCAAA+CGG + chr4_1:78226196-78226215 None:intergenic 35.0%
TGAGATGAGATGATGTATAG+TGG - chr4_1:78226249-78226268 Msa0574620:five_prime_UTR 35.0%
TCCATTTCCTTTACTCCAAA+GGG - chr4_1:78226410-78226429 Msa0574620:five_prime_UTR 35.0%
!! TATTTGACCCTTTGGAGTAA+AGG + chr4_1:78226420-78226439 None:intergenic 35.0%
TTTCACGTAAACTTCCTGCA+TGG - chr4_1:78223792-78223811 Msa0574620:three_prime_UTR 40.0%
TCTTGGTCGTGTTATGTGAT+GGG + chr4_1:78223818-78223837 None:intergenic 40.0%
TTCTTGGTCGTGTTATGTGA+TGG + chr4_1:78223819-78223838 None:intergenic 40.0%
!! ATCAAGAGATTTGGCTTTGG+CGG + chr4_1:78223939-78223958 None:intergenic 40.0%
! TTCACTCTCTTGTTTGTGCA+TGG - chr4_1:78224156-78224175 Msa0574620:CDS 40.0%
GTTTGTGTGCTTCAATTGCT+TGG + chr4_1:78224326-78224345 None:intergenic 40.0%
ATAAGCATGCCAAATCAGCA+TGG - chr4_1:78224379-78224398 Msa0574620:CDS 40.0%
TAAGCATGCCAAATCAGCAT+GGG - chr4_1:78224380-78224399 Msa0574620:CDS 40.0%
! ACAATCTTCCCATGCTGATT+TGG + chr4_1:78224391-78224410 None:intergenic 40.0%
AGTAGTTTAAGTGAGAGGGA+AGG + chr4_1:78224548-78224567 None:intergenic 40.0%
GGGAAGTAGTTTAAGTGAGA+GGG + chr4_1:78224552-78224571 None:intergenic 40.0%
TGGGAAGTAGTTTAAGTGAG+AGG + chr4_1:78224553-78224572 None:intergenic 40.0%
GTGAAGTTGCTTAACATGGA+TGG + chr4_1:78224573-78224592 None:intergenic 40.0%
!! TGAAGTTAGAGTGCTGCTAA+TGG + chr4_1:78224619-78224638 None:intergenic 40.0%
CTAAACAAAATGGAGGGAGA+AGG - chr4_1:78224670-78224689 Msa0574620:CDS 40.0%
GTCAGATAGAAAGCTTCTTC+AGG - chr4_1:78224731-78224750 Msa0574620:CDS 40.0%
TAAGACAATATCTGAGGGTG+TGG - chr4_1:78224821-78224840 Msa0574620:intron 40.0%
CTGCTAAGCTTAGTGGAAAA+GGG - chr4_1:78224850-78224869 Msa0574620:intron 40.0%
GAAAGTAGCTGAACGAAATG+TGG + chr4_1:78225015-78225034 None:intergenic 40.0%
! CACTTTTGCCCCATTAAGAT+AGG - chr4_1:78225216-78225235 Msa0574620:intron 40.0%
TGCATGTCCCTATCTTAATG+GGG + chr4_1:78225227-78225246 None:intergenic 40.0%
CTGCATGTCCCTATCTTAAT+GGG + chr4_1:78225228-78225247 None:intergenic 40.0%
ACTGCATGTCCCTATCTTAA+TGG + chr4_1:78225229-78225248 None:intergenic 40.0%
! GGACATGACCTTTGAGAATA+CGG - chr4_1:78225550-78225569 Msa0574620:CDS 40.0%
!!! AGCTACTGCTTGGTGTTTTT+GGG + chr4_1:78225582-78225601 None:intergenic 40.0%
! CATAAGCATACAAGGTGTCT+TGG + chr4_1:78225658-78225677 None:intergenic 40.0%
!! CGAAGATGTTGCAGTTTTGA+AGG + chr4_1:78225754-78225773 None:intergenic 40.0%
CTGGTGATTCTTGGATTTCA+GGG - chr4_1:78226161-78226180 Msa0574620:CDS 40.0%
GTCCATTTCCTTTACTCCAA+AGG - chr4_1:78226409-78226428 Msa0574620:five_prime_UTR 40.0%
!! ACCCTTTGGAGTAAAGGAAA+TGG + chr4_1:78226414-78226433 None:intergenic 40.0%
!! TCAAGAGATTTGGCTTTGGC+GGG + chr4_1:78223938-78223957 None:intergenic 45.0%
ATGGGAAGAGCTAGGTGTAT+AGG + chr4_1:78224035-78224054 None:intergenic 45.0%
TGCTTCAATTGCTTGGTCCA+AGG + chr4_1:78224319-78224338 None:intergenic 45.0%
GATGATGTAGATGCGGATGA+TGG + chr4_1:78224645-78224664 None:intergenic 45.0%
TCTGACCCTGAAAGCCAATA+TGG + chr4_1:78224717-78224736 None:intergenic 45.0%
AAAGCTTCTTCAGGCAACAC+CGG - chr4_1:78224740-78224759 Msa0574620:CDS 45.0%
GATATCGTGGTTGCACAAGA+TGG - chr4_1:78224786-78224805 Msa0574620:intron 45.0%
TGGTTGCACAAGATGGAAGT+GGG - chr4_1:78224793-78224812 Msa0574620:intron 45.0%
GCTGCTAAGCTTAGTGGAAA+AGG - chr4_1:78224849-78224868 Msa0574620:intron 45.0%
ATTGCAGCTGTTATGGGAGA+TGG - chr4_1:78225515-78225534 Msa0574620:CDS 45.0%
GGGAGATGGTTTCATAGCTA+AGG - chr4_1:78225529-78225548 Msa0574620:CDS 45.0%
! ATGACCTTTGAGAATACGGC+AGG - chr4_1:78225554-78225573 Msa0574620:CDS 45.0%
!!! GCTACTGCTTGGTGTTTTTG+GGG + chr4_1:78225581-78225600 None:intergenic 45.0%
!!! GAGCTACTGCTTGGTGTTTT+TGG + chr4_1:78225583-78225602 None:intergenic 45.0%
ACGGTAGAAAACGGAGTGAT+CGG + chr4_1:78225618-78225637 None:intergenic 45.0%
! GAAGGCACAACGGTAGAAAA+CGG + chr4_1:78225627-78225646 None:intergenic 45.0%
TTCTACCGTTGTGCCTTCAA+AGG - chr4_1:78225629-78225648 Msa0574620:CDS 45.0%
ACGGTTGGCATAAGCATACA+AGG + chr4_1:78225666-78225685 None:intergenic 45.0%
! GTGTTCTGTTGGTTGCTCAT+TGG + chr4_1:78225785-78225804 None:intergenic 45.0%
! TGTTCTTCCCTGTGCTGTTA+CGG + chr4_1:78225807-78225826 None:intergenic 45.0%
AATGCAAAACCACCCATCCA+TGG + chr4_1:78226007-78226026 None:intergenic 45.0%
GCTGGTGATTCTTGGATTTC+AGG - chr4_1:78226160-78226179 Msa0574620:CDS 45.0%
GATTCTTGGATTTCAGGGAG+TGG - chr4_1:78226166-78226185 Msa0574620:CDS 45.0%
!! TGTTATGTGATGGGCCATGC+AGG + chr4_1:78223809-78223828 None:intergenic 50.0%
TGGTTGCACACATGAGGGTA+TGG + chr4_1:78224207-78224226 None:intergenic 50.0%
AAGCTTCTTCAGGCAACACC+GGG - chr4_1:78224741-78224760 Msa0574620:CDS 50.0%
!! AGGACCAAGTGCTGATATCG+TGG - chr4_1:78224773-78224792 Msa0574620:CDS 50.0%
GCAACCACGATATCAGCACT+TGG + chr4_1:78224780-78224799 None:intergenic 50.0%
GTGGTTGCACAAGATGGAAG+TGG - chr4_1:78224792-78224811 Msa0574620:intron 50.0%
GATGCAGCTGCTAAGCTTAG+TGG - chr4_1:78224843-78224862 Msa0574620:intron 50.0%
! GGTAGCCTTTGAAGGCACAA+CGG + chr4_1:78225637-78225656 None:intergenic 50.0%
! GGTGTCTTGGTAGCCTTTGA+AGG + chr4_1:78225645-78225664 None:intergenic 50.0%
TGCAGTCGCGGTAGAATTGA+CGG + chr4_1:78225685-78225704 None:intergenic 50.0%
TACCGCGACTGCAACATCTA+CGG - chr4_1:78225692-78225711 Msa0574620:CDS 50.0%
TTCCGTAGATGTTGCAGTCG+CGG + chr4_1:78225697-78225716 None:intergenic 50.0%
! GTGCTGTTACGGTGTTCTGT+TGG + chr4_1:78225796-78225815 None:intergenic 50.0%
ACAGAACACCGTAACAGCAC+AGG - chr4_1:78225796-78225815 Msa0574620:CDS 50.0%
CAGAACACCGTAACAGCACA+GGG - chr4_1:78225797-78225816 Msa0574620:CDS 50.0%
ATTCAGAAGGTTGGGCTCCA+TGG - chr4_1:78225987-78226006 Msa0574620:CDS 50.0%
!! GTTGGTGGTGGTGCTAATAC+TGG - chr4_1:78226058-78226077 Msa0574620:CDS 50.0%
GGTGGAAGAGTTAATTGGCC+TGG - chr4_1:78226079-78226098 Msa0574620:CDS 50.0%
!! TTGGCTTTGGCGGGCCATAA+AGG + chr4_1:78223929-78223948 None:intergenic 55.0%
AGCTTCTTCAGGCAACACCG+GGG - chr4_1:78224742-78224761 Msa0574620:CDS 55.0%
GGGTCCTGCCGTATTCTCAA+AGG + chr4_1:78225561-78225580 None:intergenic 55.0%
CAAGCAGTAGCTCTCCGTTC+CGG - chr4_1:78225590-78225609 Msa0574620:CDS 55.0%
GTCGCGGTAGAATTGACGGT+TGG + chr4_1:78225681-78225700 None:intergenic 55.0%
AGAAGGTTGGGCTCCATGGA+TGG - chr4_1:78225991-78226010 Msa0574620:CDS 55.0%
GAAGGTTGGGCTCCATGGAT+GGG - chr4_1:78225992-78226011 Msa0574620:CDS 55.0%
! GGTGGTGGTGCTAATACTGG+TGG - chr4_1:78226061-78226080 Msa0574620:CDS 55.0%
!! AGTGGTGTGCCGTTTGATGC+AGG - chr4_1:78226184-78226203 Msa0574620:CDS 55.0%
TTCAGGCAACACCGGGGTCA+GGG - chr4_1:78224748-78224767 Msa0574620:CDS 60.0%
CGGAACGGAGAGCTACTGCT+TGG + chr4_1:78225592-78225611 None:intergenic 60.0%
GAAAACGGAGTGATCGGCGC+CGG + chr4_1:78225612-78225631 None:intergenic 60.0%
CTTCAGGCAACACCGGGGTC+AGG - chr4_1:78224747-78224766 Msa0574620:CDS 65.0%
CTTGGTCCTGTCCCTGACCC+CGG + chr4_1:78224762-78224781 None:intergenic 65.0%
GGTTGGGCTCCATGGATGGG+TGG - chr4_1:78225995-78226014 Msa0574620:CDS 65.0%
GCAACACCGGGGTCAGGGAC+AGG - chr4_1:78224753-78224772 Msa0574620:CDS 70.0%
! CGGAGTGATCGGCGCCGGAA+CGG + chr4_1:78225607-78225626 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 78223784 78226538 78223784 ID=Msa0574620;Name=Msa0574620
chr4_1 mRNA 78223784 78226538 78223784 ID=Msa0574620-mRNA-1;Parent=Msa0574620;Name=Msa0574620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=303|1|1|1|1|1|2|325|546
chr4_1 exon 78223784 78224800 78223784 ID=Msa0574620-mRNA-1:exon:27725;Parent=Msa0574620-mRNA-1
chr4_1 exon 78225287 78226538 78225287 ID=Msa0574620-mRNA-1:exon:27724;Parent=Msa0574620-mRNA-1
chr4_1 five_prime_UTR 78226236 78226538 78226236 ID=Msa0574620-mRNA-1:five_prime_utr;Parent=Msa0574620-mRNA-1
chr4_1 CDS 78225287 78226235 78225287 ID=Msa0574620-mRNA-1:cds;Parent=Msa0574620-mRNA-1
chr4_1 CDS 78224109 78224800 78224109 ID=Msa0574620-mRNA-1:cds;Parent=Msa0574620-mRNA-1
chr4_1 three_prime_UTR 78223784 78224108 78223784 ID=Msa0574620-mRNA-1:three_prime_utr;Parent=Msa0574620-mRNA-1
Gene Sequence

>Msa0574620

ATGCACACTATTTCATTTTCTAATATGGATAAACTCATATTCAACTCTTGCATTGCATACTTTCTACTTTTCACTCTCTTGTTTGTGCATGGCAAAGAATTGTCATGCAATCAAACTCCATACCCTCATGTGTGCAACCATTATATTGGAACCACCACAAATAAACTATCAACCCTAGATTCTTCTTCATCATCTTCTTCTTCCTTCCATGATATTGCACTTAAGGTTACCTTGGACCAAGCAATTGAAGCACACAAACTTGTATCAACCATGGAATTAAATAATTTCAAAGATAAGCATGCCAAATCAGCATGGGAAGATTGTTTAGAGCTTTATGAGGATACAATATATCAACTCAAACGCTCAATAAACTCAAACAACCTAAATGACAAATTAACTTGGCAAAGTGCTTCCATTACCAATCATCAAACTTGTCAAAATGGTTTCATTGATTTCAACCTTCCCTCTCACTTAAACTACTTCCCATCCATGTTAAGCAACTTCACTAAATTGCTTAGTAATTCTTTGTCCATTAGCAGCACTCTAACTTCATCACCATCATCCGCATCTACATCATCAAGTACTAAACAAAATGGAGGGAGAAGGTTGTTATCAGATAATAAATTTCCATATTGGCTTTCAGGGTCAGATAGAAAGCTTCTTCAGGCAACACCGGGGTCAGGGACAGGACCAAGTGCTGATATCGTGGTTGCACAAGATGGAAGTGGGAACTATAAGACAATATCTGAGGGTGTGGATGCAGCTGCTAAGCTTAGTGGAAAAGGGAGAGTTGTTATTCATCTGAAAGCAGGTGTTTATAAAGAGAATGTTGATATCAAAAGGACAGTGACAAATTTAATGATATTTGGAGATGGAATGGATTCTACTATTGTCACTGGTAATCAAAATGCTATAGATGGTTCTACCACATTTCGTTCAGCTACTTTCGCTGTTATGGGAGATGGTTTCATAGCTAAGGACATGACCTTTGAGAATACGGCAGGACCCCAAAAACACCAAGCAGTAGCTCTCCGTTCCGGCGCCGATCACTCCGTTTTCTACCGTTGTGCCTTCAAAGGCTACCAAGACACCTTGTATGCTTATGCCAACCGTCAATTCTACCGCGACTGCAACATCTACGGAACAATTGATTTCATCTTCGGTAACGCAGTTACTGTCCTTCAAAACTGCAACATCTTCGTTAGAAAACCAATGAGCAACCAACAGAACACCGTAACAGCACAGGGAAGAACAGATCCAAATGAAAACACAGGAATTGTAATACATAACTGCCGTATAACCGCATCAAATGATTTGAAACCGGTTCAGAATTCAGTTGAAACTTATCTTGGTAGACCATGGCAGAAATATTCAAGAACGGTTGTAATGAAAAGTAATATTGATGGTTTGATAAATTCAGAAGGTTGGGCTCCATGGATGGGTGGTTTTGCATTGAGTACACTTTATTATGGTGAATATATGAATGTTGGTGGTGGTGCTAATACTGGTGGAAGAGTTAATTGGCCTGGTTTTCATGTTATTACTAATCCTTCTGAGGCTGTTAAATTTTCTGTTGGTAATTTTTTGGCTGGTGATTCTTGGATTTCAGGGAGTGGTGTGCCGTTTGATGCAGGTTTGTGA
Protein sequence

>Msa0574620

MHTISFSNMDKLIFNSCIAYFLLFTLLFVHGKELSCNQTPYPHVCNHYIGTTTNKLSTLDSSSSSSSSFHDIALKVTLDQAIEAHKLVSTMELNNFKDKHAKSAWEDCLELYEDTIYQLKRSINSNNLNDKLTWQSASITNHQTCQNGFIDFNLPSHLNYFPSMLSNFTKLLSNSLSISSTLTSSPSSASTSSSTKQNGGRRLLSDNKFPYWLSGSDRKLLQATPGSGTGPSADIVVAQDGSGNYKTISEGVDAAAKLSGKGRVVIHLKAGVYKENVDIKRTVTNLMIFGDGMDSTIVTGNQNAIDGSTTFRSATFAVMGDGFIAKDMTFENTAGPQKHQAVALRSGADHSVFYRCAFKGYQDTLYAYANRQFYRDCNIYGTIDFIFGNAVTVLQNCNIFVRKPMSNQQNTVTAQGRTDPNENTGIVIHNCRITASNDLKPVQNSVETYLGRPWQKYSRTVVMKSNIDGLINSEGWAPWMGGFALSTLYYGEYMNVGGGANTGGRVNWPGFHVITNPSEAVKFSVGNFLAGDSWISGSGVPFDAGL*