AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0708330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708330 MtrunA17_Chr8g0391311 93.956 546 28 2 1 546 1 541 0.0 1050
Msa0708330 MtrunA17_Chr8g0391291 93.773 546 27 3 1 546 1 539 0.0 1046
Msa0708330 MtrunA17_Chr8g0391281 92.089 316 18 3 1 316 1 309 0.0 568
Msa0708330 MtrunA17_Chr4g0011771 50.092 541 230 11 13 546 6 513 0.0 525
Msa0708330 MtrunA17_Chr4g0011791 49.257 538 229 13 18 546 7 509 6.51e-178 511
Msa0708330 MtrunA17_Chr4g0018371 44.402 518 263 6 36 546 40 539 2.11e-160 468
Msa0708330 MtrunA17_Chr3g0090091 46.435 519 244 8 36 546 36 528 2.29e-160 467
Msa0708330 MtrunA17_Chr3g0079341 45.962 520 244 7 36 546 36 527 2.85e-159 464
Msa0708330 MtrunA17_Chr3g0079361 45.665 519 247 6 36 546 36 527 9.29e-158 460
Msa0708330 MtrunA17_Chr3g0079351 45.962 520 244 9 36 546 36 527 1.01e-156 457
Msa0708330 MtrunA17_Chr4g0011751 51.002 449 190 7 104 546 65 489 1.33e-156 456
Msa0708330 MtrunA17_Chr7g0269541 49.889 451 207 5 102 542 111 552 2.20e-153 450
Msa0708330 MtrunA17_Chr7g0233771 45.644 528 249 11 37 546 47 554 1.80e-152 448
Msa0708330 MtrunA17_Chr8g0345281 44.612 529 266 7 35 546 71 589 1.15e-148 439
Msa0708330 MtrunA17_Chr3g0126771 42.777 533 268 8 35 546 81 597 9.94e-147 435
Msa0708330 MtrunA17_Chr1g0147051 42.593 540 265 12 35 546 85 607 1.06e-142 425
Msa0708330 MtrunA17_Chr2g0302021 46.751 477 219 7 83 546 71 525 5.91e-142 420
Msa0708330 MtrunA17_Chr7g0233801 42.830 523 260 11 42 546 52 553 1.20e-140 417
Msa0708330 MtrunA17_Chr8g0354901 49.465 467 204 9 92 546 135 581 3.77e-140 417
Msa0708330 MtrunA17_Chr7g0233831 46.957 460 222 7 92 546 113 555 1.31e-139 415
Msa0708330 MtrunA17_Chr7g0272141 44.779 498 236 10 65 546 68 542 2.18e-138 411
Msa0708330 MtrunA17_Chr7g0269531 43.519 540 257 10 33 542 40 561 4.18e-137 409
Msa0708330 MtrunA17_Chr4g0069841 45.208 480 217 8 85 546 65 516 6.47e-137 407
Msa0708330 MtrunA17_Chr1g0192331 45.009 531 240 14 35 546 31 528 1.75e-136 406
Msa0708330 MtrunA17_Chr4g0069891 42.026 533 269 10 33 546 82 593 5.90e-134 402
Msa0708330 MtrunA17_Chr7g0269521 49.184 429 199 6 133 546 138 562 1.06e-133 400
Msa0708330 MtrunA17_Chr3g0080391 46.813 455 212 8 103 546 139 574 5.95e-133 399
Msa0708330 MtrunA17_Chr7g0269511 43.208 530 277 8 35 546 41 564 1.93e-131 394
Msa0708330 MtrunA17_Chr1g0195331 43.063 555 283 11 18 546 8 555 9.03e-131 392
Msa0708330 MtrunA17_Chr4g0069881 42.308 520 263 12 36 546 44 535 1.33e-130 391
Msa0708330 MtrunA17_Chr1g0195301 42.703 555 285 11 18 546 8 555 2.54e-130 391
Msa0708330 MtrunA17_Chr1g0195321 42.703 555 285 11 18 546 8 555 2.54e-130 391
Msa0708330 MtrunA17_Chr8g0345211 42.830 530 256 13 36 546 81 582 4.05e-130 392
Msa0708330 MtrunA17_Chr4g0069861 43.271 483 224 7 85 546 64 517 4.10e-130 389
Msa0708330 MtrunA17_Chr1g0175661 42.287 551 286 10 21 546 15 558 3.27e-129 388
Msa0708330 MtrunA17_Chr8g0354911 41.123 552 269 12 35 546 77 612 4.48e-127 385
Msa0708330 MtrunA17_Chr1g0194091 39.854 547 291 11 27 546 34 569 4.56e-126 381
Msa0708330 MtrunA17_Chr1g0195351 42.342 555 285 12 18 546 8 553 2.56e-125 378
Msa0708330 MtrunA17_Chr1g0195311 61.303 261 100 1 286 546 55 314 1.50e-117 350
Msa0708330 MtrunA17_Chr1g0194081 38.677 499 286 10 63 546 70 563 3.87e-117 357
Msa0708330 MtrunA17_Chr3g0080381 38.559 555 281 13 35 546 91 628 1.30e-116 358
Msa0708330 MtrunA17_Chr1g0192311 38.889 522 287 12 36 542 62 566 3.15e-114 350
Msa0708330 MtrunA17_Chr1g0192281 38.889 522 287 12 36 542 62 566 3.15e-114 350
Msa0708330 MtrunA17_Chr1g0192301 39.630 487 265 10 72 542 98 571 3.70e-112 345
Msa0708330 MtrunA17_Chr1g0192321 37.684 544 284 14 36 545 60 582 2.08e-111 343
Msa0708330 MtrunA17_Chr7g0272151 39.563 503 263 11 72 545 92 582 2.04e-110 341
Msa0708330 MtrunA17_Chr4g0000121 39.425 487 266 10 72 542 80 553 2.49e-110 340
Msa0708330 MtrunA17_Chr4g0067611 39.469 527 284 13 36 545 63 571 6.19e-109 337
Msa0708330 MtrunA17_Chr4g0067601 39.245 530 287 14 33 545 60 571 2.18e-107 333
Msa0708330 MtrunA17_Chr7g0270661 39.511 491 265 9 79 541 83 569 1.02e-106 331
Msa0708330 MtrunA17_Chr7g0270671 39.511 491 265 9 79 541 83 569 1.02e-106 331
Msa0708330 MtrunA17_Chr2g0301931 53.521 284 127 3 263 546 14 292 1.64e-104 315
Msa0708330 MtrunA17_Chr7g0234711 62.281 228 85 1 320 546 42 269 4.04e-103 311
Msa0708330 MtrunA17_Chr7g0270651 37.160 514 291 9 61 546 65 574 5.14e-103 321
Msa0708330 MtrunA17_Chr2g0295531 35.606 528 293 12 33 542 44 542 6.63e-103 322
Msa0708330 MtrunA17_Chr7g0270691 36.875 480 262 9 88 546 90 549 7.01e-100 313
Msa0708330 MtrunA17_Chr2g0295551 37.453 534 286 12 33 545 57 563 7.58e-100 313
Msa0708330 MtrunA17_Chr2g0302001 53.543 254 117 1 293 546 185 437 2.99e-99 307
Msa0708330 MtrunA17_Chr7g0270631 38.398 487 268 9 79 537 71 553 1.07e-94 312
Msa0708330 MtrunA17_Chr7g0270631 37.759 482 265 10 79 529 619 1096 5.92e-84 282
Msa0708330 MtrunA17_Chr8g0391261 92.806 139 10 0 408 546 28 166 6.95e-92 278
Msa0708330 MtrunA17_Chr8g0384121 33.585 530 304 12 31 538 53 556 6.89e-90 287
Msa0708330 MtrunA17_Chr3g0129921 33.692 558 304 16 20 539 28 557 3.62e-89 285
Msa0708330 MtrunA17_Chr2g0295541 35.872 499 253 11 98 545 112 594 4.52e-89 286
Msa0708330 MtrunA17_Chr8g0380581 40.059 337 192 4 208 542 44 372 4.08e-85 268
Msa0708330 MtrunA17_Chr5g0437831 41.888 339 190 6 210 546 50 383 4.30e-84 266
Msa0708330 MtrunA17_Chr7g0268241 40.506 316 175 5 232 546 47 350 1.02e-77 248
Msa0708330 MtrunA17_Chr2g0334111 38.854 314 177 5 234 546 24 323 2.70e-76 244
Msa0708330 MtrunA17_Chr7g0271381 30.684 541 297 18 36 546 64 556 1.07e-67 228
Msa0708330 MtrunA17_Chr6g0487101 34.177 395 229 11 156 535 2 380 1.17e-52 183
Msa0708330 MtrunA17_Chr8g0389771 35.447 347 192 10 205 535 47 377 3.16e-50 177
Msa0708330 MtrunA17_Chr6g0453551 33.443 305 178 6 235 529 6 295 5.96e-49 172
Msa0708330 MtrunA17_Chr1g0194311 32.132 333 195 8 213 535 57 368 9.55e-49 172
Msa0708330 MtrunA17_Chr6g0466951 34.722 288 164 6 226 508 60 328 2.71e-48 171
Msa0708330 MtrunA17_Chr2g0299431 42.857 245 125 6 208 447 30 264 4.00e-48 169
Msa0708330 MtrunA17_Chr7g0258341 31.620 389 231 9 158 536 25 388 2.82e-47 169
Msa0708330 MtrunA17_Chr6g0466961 35.862 290 158 7 226 508 31 299 9.39e-47 166
Msa0708330 MtrunA17_Chr7g0217941 32.099 324 189 7 237 544 8 316 1.72e-46 165
Msa0708330 MtrunA17_Chr1g0161731 29.683 347 199 8 201 539 28 337 2.18e-42 155
Msa0708330 MtrunA17_Chr7g0234731 33.101 287 158 6 36 315 55 314 1.07e-41 152
Msa0708330 MtrunA17_Chr4g0070051 31.833 311 181 12 235 535 54 343 2.32e-37 141
Msa0708330 MtrunA17_Chr5g0446301 32.248 307 180 7 241 535 67 357 2.42e-37 141
Msa0708330 MtrunA17_Chr7g0265201 30.065 306 186 6 237 535 101 385 9.90e-37 140
Msa0708330 MtrunA17_Chr5g0440871 31.579 304 183 7 235 536 39 319 4.82e-36 137
Msa0708330 MtrunA17_Chr5g0437821 46.970 132 69 1 349 479 4 135 2.01e-35 129
Msa0708330 MtrunA17_Chr5g0399221 30.091 329 196 9 230 544 56 364 7.18e-35 134
Msa0708330 MtrunA17_Chr2g0294321 29.042 334 213 7 210 535 29 346 6.16e-34 132
Msa0708330 MtrunA17_Chr8g0351871 28.707 317 197 8 229 536 27 323 1.20e-31 125
Msa0708330 MtrunA17_Chr4g0037751 29.866 298 185 7 214 505 55 334 3.32e-31 124
Msa0708330 MtrunA17_Chr6g0459781 38.333 180 95 5 235 401 13 189 1.27e-30 118
Msa0708330 MtrunA17_Chr2g0295851 28.358 335 205 10 210 535 28 336 4.80e-30 120
Msa0708330 MtrunA17_Chr5g0420831 30.035 283 167 8 235 505 71 334 4.38e-29 118
Msa0708330 MtrunA17_Chr4g0025841 28.846 260 167 5 220 479 22 263 4.37e-26 107
Msa0708330 MtrunA17_Chr5g0440901 33.553 152 91 2 328 479 1 142 9.83e-23 94.7
Msa0708330 MtrunA17_Chr7g0265161 31.073 177 89 4 305 476 9 157 2.25e-20 90.5
Msa0708330 MtrunA17_Chr2g0314691 31.176 170 105 3 339 507 18 176 6.45e-20 88.2
Msa0708330 MtrunA17_Chr6g0460141 36.957 138 79 4 266 395 7 144 5.56e-19 84.0
Msa0708330 MtrunA17_Chr5g0440891 35.032 157 92 4 235 390 48 195 1.34e-18 84.3
Msa0708330 MtrunA17_Chr4g0073911 27.841 176 117 3 266 434 38 210 4.05e-16 78.2
Msa0708330 MtrunA17_Chr6g0459791 36.000 125 67 4 412 535 4 116 1.65e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708330 AT1G23200.1 53.659 533 211 12 35 546 37 554 0.0 551
Msa0708330 AT2G45220.1 49.611 514 220 11 36 546 34 511 1.48e-172 498
Msa0708330 AT3G49220.1 45.038 524 259 11 35 546 90 596 1.20e-148 440
Msa0708330 AT3G49220.3 45.038 524 259 11 35 546 135 641 3.47e-148 440
Msa0708330 AT5G51490.1 42.395 526 261 9 36 546 38 536 6.44e-147 433
Msa0708330 AT3G60730.1 45.436 493 242 6 74 546 34 519 8.03e-147 432
Msa0708330 AT5G53370.1 43.678 522 270 9 35 546 78 585 1.20e-146 434
Msa0708330 AT5G53370.2 43.678 522 270 9 35 546 114 621 3.65e-146 434
Msa0708330 AT5G51500.1 43.346 526 256 11 36 546 42 540 9.97e-146 431
Msa0708330 AT3G47400.1 42.966 526 266 9 36 546 88 594 1.03e-143 427
Msa0708330 AT3G14310.1 42.518 548 262 9 35 546 62 592 1.79e-142 424
Msa0708330 AT1G11580.1 45.570 474 230 6 86 546 99 557 1.14e-139 416
Msa0708330 AT1G53830.1 42.778 540 262 9 36 546 66 587 1.57e-139 416
Msa0708330 AT1G11580.2 45.570 474 230 6 86 546 120 578 3.39e-139 415
Msa0708330 AT3G59010.1 46.791 483 225 11 73 546 69 528 3.26e-138 411
Msa0708330 AT4G00190.1 42.748 524 245 11 28 546 1 474 9.92e-136 402
Msa0708330 AT2G26440.1 43.048 525 267 10 36 546 41 547 1.03e-134 402
Msa0708330 AT4G02320.1 46.993 449 225 6 104 546 77 518 9.59e-134 399
Msa0708330 AT4G02330.1 41.913 575 276 14 18 546 9 571 1.08e-133 400
Msa0708330 AT4G02300.1 42.065 523 253 10 56 546 28 532 5.57e-133 397
Msa0708330 AT3G43270.1 45.287 488 241 7 73 546 50 525 1.27e-132 396
Msa0708330 AT2G26450.1 42.337 522 267 12 36 546 113 611 3.25e-132 398
Msa0708330 AT3G05620.1 43.320 494 234 9 73 546 74 541 3.96e-131 393
Msa0708330 AT4G33220.1 38.378 555 285 8 16 546 2 523 6.75e-131 392
Msa0708330 AT5G27870.1 41.379 522 275 12 36 546 61 562 2.25e-130 397
Msa0708330 AT2G43050.1 43.277 476 242 6 74 546 66 516 2.74e-130 390
Msa0708330 AT5G04960.1 41.553 515 271 8 36 544 72 562 4.99e-130 391
Msa0708330 AT3G10710.1 41.154 520 265 10 36 546 74 561 1.92e-125 379
Msa0708330 AT3G05610.1 40.494 526 276 14 36 546 62 565 5.75e-125 381
Msa0708330 AT4G33220.2 45.185 405 207 4 147 546 8 402 1.35e-124 371
Msa0708330 AT1G02810.1 39.086 591 276 14 21 546 6 577 8.33e-124 375
Msa0708330 AT5G49180.1 40.417 527 281 13 33 542 56 566 1.93e-123 374
Msa0708330 AT2G47550.1 45.000 480 234 10 92 546 84 558 4.51e-122 370
Msa0708330 AT3G10720.2 39.227 543 290 7 35 546 82 615 8.90e-122 372
Msa0708330 AT3G06830.1 40.546 513 277 12 51 544 63 566 4.29e-121 368
Msa0708330 AT5G04970.1 40.219 547 281 10 34 546 86 620 4.52e-121 370
Msa0708330 AT4G33230.1 40.153 523 286 11 36 546 99 606 8.81e-121 369
Msa0708330 AT1G53840.1 38.418 531 275 14 36 540 77 581 5.97e-118 360
Msa0708330 AT5G20860.2 41.319 455 227 9 106 546 110 538 2.44e-116 355
Msa0708330 AT3G14300.1 39.544 526 276 13 36 541 463 966 3.12e-115 364
Msa0708330 AT3G10720.1 56.538 260 112 1 287 546 1 259 9.64e-108 323
Msa0708330 AT4G03930.1 36.314 548 296 12 20 546 9 524 1.35e-104 324
Msa0708330 AT4G15980.1 47.246 345 178 4 201 544 359 700 7.15e-104 328
Msa0708330 AT4G15980.2 47.246 345 178 4 201 544 369 710 9.83e-104 328
Msa0708330 AT3G27980.1 47.478 337 169 3 210 546 169 497 5.90e-103 319
Msa0708330 AT1G11590.1 40.045 447 238 7 110 546 98 524 4.77e-102 318
Msa0708330 AT5G20860.1 38.215 437 202 8 103 525 107 489 1.44e-95 300
Msa0708330 AT5G09760.1 33.955 536 295 12 33 539 46 551 1.00e-91 291
Msa0708330 AT5G09760.2 33.955 536 295 12 33 539 72 577 2.04e-91 291
Msa0708330 AT1G11370.1 50.000 258 128 1 221 478 32 288 9.08e-88 273
Msa0708330 AT2G47030.1 42.368 380 194 8 185 546 216 588 9.58e-87 280
Msa0708330 AT2G47040.1 38.901 455 249 13 107 546 155 595 4.60e-86 278
Msa0708330 AT5G64640.1 33.143 525 311 13 33 536 94 599 4.70e-86 278
Msa0708330 AT3G62170.1 37.815 476 252 12 104 546 124 588 5.04e-86 278
Msa0708330 AT5G19730.1 36.278 317 174 8 231 535 81 381 1.89e-51 181
Msa0708330 AT3G29090.1 34.507 284 164 4 235 508 7 278 9.17e-49 171
Msa0708330 AT1G05310.1 34.259 324 184 7 225 535 81 388 1.52e-47 171
Msa0708330 AT2G36710.1 33.117 308 186 5 235 535 91 385 1.42e-44 163
Msa0708330 AT2G36700.1 31.579 304 189 5 237 535 44 333 2.18e-44 160
Msa0708330 AT1G44980.1 44.211 190 104 2 210 399 54 241 1.07e-41 150
Msa0708330 AT3G17060.1 29.677 310 190 7 235 535 46 336 5.34e-40 149
Msa0708330 AT2G21610.1 30.132 302 190 6 235 535 51 332 1.98e-39 147
Msa0708330 AT2G21610.2 30.132 302 190 6 235 535 56 337 3.07e-39 147
Msa0708330 AT3G24130.1 31.818 286 170 6 234 508 33 304 3.75e-39 146
Msa0708330 AT5G18990.1 32.000 300 172 7 220 508 21 299 5.14e-38 143
Msa0708330 AT5G47500.1 30.909 330 194 9 229 544 53 362 1.76e-37 142
Msa0708330 AT5G07430.1 30.492 305 192 6 235 535 67 355 1.30e-36 140
Msa0708330 AT5G55590.1 33.758 314 178 9 235 535 82 378 6.85e-35 135
Msa0708330 AT5G26810.1 30.476 315 192 9 235 536 34 334 3.80e-34 132
Msa0708330 AT3G49220.2 32.993 294 168 11 35 316 135 411 9.42e-34 133
Msa0708330 AT2G19150.1 29.268 328 201 8 226 542 32 339 2.84e-33 130
Msa0708330 AT5G61680.1 29.085 306 196 7 235 535 69 358 4.39e-33 130
Msa0708330 AT5G55590.2 33.852 257 145 7 235 479 82 325 4.41e-33 129
Msa0708330 AT5G07420.1 29.450 309 190 10 235 535 67 355 3.42e-31 124
Msa0708330 AT2G47280.1 28.617 311 196 7 235 536 35 328 2.63e-29 119
Msa0708330 AT2G19150.2 30.418 263 159 6 226 479 32 279 5.69e-29 116
Msa0708330 AT1G69940.1 26.510 298 198 7 243 535 74 355 2.05e-27 114
Msa0708330 AT5G07410.1 26.174 298 199 7 243 535 74 355 2.42e-26 110

Find 165 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCATATTGGCTTTC+AGG 0.136783 4_4:-81873483 Msa0708330:CDS
GCTGGTGATTCTTGGATTTC+AGG 0.138272 4_4:-81871657 Msa0708330:CDS
GTGACAAATTTAATGATATT+TGG 0.156463 4_4:-81873258 Msa0708330:CDS
CACAAATTAGTATACCTTTA+TGG 0.175493 4_4:-81874279 Msa0708330:five_prime_UTR
GCCAAGTTAATTTGTCATTT+AGG 0.213459 4_4:+81873724 None:intergenic
AATAATTATTGCAGCTGTTA+TGG 0.232312 4_4:-81872309 Msa0708330:intron
CTAACATTTCACAAAGTTCT+TGG 0.238259 4_4:+81874359 None:intergenic
GCTTTGAGTACACTTTATTA+TGG 0.257286 4_4:-81871795 Msa0708330:CDS
CTTCCATGATATTGCACTTA+AGG 0.261043 4_4:-81873901 Msa0708330:CDS
AATCATCAAACTTGTCAAAA+TGG 0.271720 4_4:-81873684 Msa0708330:CDS
ACAATCTTCCCATGCTGATT+TGG 0.281247 4_4:+81873803 None:intergenic
CAGATAATAAATTTCCATAT+TGG 0.306714 4_4:-81873491 Msa0708330:CDS
AATCATTTGCTGCGGTTATA+CGG 0.315468 4_4:+81871952 None:intergenic
TCTGACCCTGAAAGCCAATA+TGG 0.325074 4_4:+81873477 None:intergenic
TAATTTGTGGGTGTAAGTTT+AGG 0.327047 4_4:+81874293 None:intergenic
AAGATGATGAAGAAGAATCT+AGG 0.330716 4_4:+81873931 None:intergenic
CTGGTGATTCTTGGATTTCA+GGG 0.333928 4_4:-81871656 Msa0708330:CDS
GTCCATTTCCTTTACTCAAA+AGG 0.344821 4_4:-81871399 Msa0708330:three_prime_UTR
TTGTGGTGGTTCCAATATAA+TGG 0.354156 4_4:+81873967 None:intergenic
ATAATTATTGCAGCTGTTAT+GGG 0.354501 4_4:-81872308 Msa0708330:intron
TCATCAAGTACTAAACAAAA+TGG 0.356786 4_4:-81873531 Msa0708330:CDS
GACTTTGGTGAAAGCATATA+TGG 0.366380 4_4:-81871523 Msa0708330:three_prime_UTR
GGAACTATTGATTTCATCTT+CGG 0.369134 4_4:-81872104 Msa0708330:CDS
TTGGCTTTGGCGGGCCATAA+AGG 0.377975 4_4:+81874265 None:intergenic
CTTCAGGCAACACCGGGGTC+AGG 0.382501 4_4:-81873444 Msa0708330:CDS
TAATTGAAGTCTATAAAGAT+AGG 0.382638 4_4:-81871494 Msa0708330:three_prime_UTR
AATTTCCATATTGGCTTTCA+GGG 0.383402 4_4:-81873482 Msa0708330:CDS
AATGGTCATATTGTATTGTA+TGG 0.386077 4_4:+81874323 None:intergenic
TATATATAGATCAAGAGATT+TGG 0.395187 4_4:+81874246 None:intergenic
AATTCAGTTAAAACATATCT+TGG 0.398697 4_4:-81871915 Msa0708330:CDS
GGTAATCAAAATGCTATAGA+TGG 0.400131 4_4:-81873207 Msa0708330:CDS
TCCATTTCCTTTACTCAAAA+GGG 0.400483 4_4:-81871398 Msa0708330:three_prime_UTR
TTTGATAAATTCAGAAGGTT+GGG 0.405145 4_4:-81871838 Msa0708330:CDS
CATGTGTGCAACCATTATAT+TGG 0.409559 4_4:-81873978 Msa0708330:CDS
ACATTTCGTTCAGCTACTTT+CGG 0.410470 4_4:-81873177 Msa0708330:intron
TGTTGCAGTCGCGGTAGAAT+TGG 0.411073 4_4:+81872132 None:intergenic
TGTTCTTCCTTGTGCTGTTA+CGG 0.414972 4_4:+81872013 None:intergenic
ATACTGGTGGAAGAGTTAAT+TGG 0.417090 4_4:-81871743 Msa0708330:CDS
GTAATGAAAAGTAATATTGA+TGG 0.420796 4_4:-81871861 Msa0708330:CDS
GCGGTTGGCGTAAGCATATA+AGG 0.426322 4_4:+81872154 None:intergenic
TAGGGTTGATAGTTTATTTG+TGG 0.428236 4_4:+81873950 None:intergenic
GCTGCTAAGCTTAGTGGAAA+AGG 0.429873 4_4:-81873342 Msa0708330:CDS
CGCAGCAAATGATTTGAAAC+CGG 0.431620 4_4:-81871943 Msa0708330:CDS
TTCACTCTCTTGTTTGTGCA+TGG 0.431690 4_4:-81874035 Msa0708330:CDS
TACAAGTTTGTGTGCTTCAA+TGG 0.434838 4_4:+81873863 None:intergenic
ATGGGAAGAGCTAGGTGTAT+AGG 0.434996 4_4:+81874159 None:intergenic
GTTGGTGGTGGTGCTAATAC+TGG 0.437055 4_4:-81871759 Msa0708330:CDS
TGAGATGATGTATAGTATAG+TGG 0.439833 4_4:-81871563 Msa0708330:three_prime_UTR
CAAGCAGTAGCTCTCCGTTC+CGG 0.442034 4_4:-81872227 Msa0708330:CDS
GTCAGATAGAAAGCTTCTTC+AGG 0.443922 4_4:-81873460 Msa0708330:CDS
GGTGTCTTGGTAGCCTTTGA+AGG 0.444283 4_4:+81872175 None:intergenic
GTTTGATAAATTCAGAAGGT+TGG 0.445073 4_4:-81871839 Msa0708330:CDS
ATCAAGAGATTTGGCTTTGG+CGG 0.445220 4_4:+81874255 None:intergenic
TTTGAAATTATTTAATTCCA+TGG 0.450557 4_4:+81873836 None:intergenic
TTGAGTAGTAATAATAAAAG+AGG 0.451056 4_4:+81871277 None:intergenic
GTGTTCTGTTGGTTGCTCAT+TGG 0.453378 4_4:+81872035 None:intergenic
GCAACACCGGGGTCAGGGAC+AGG 0.454024 4_4:-81873438 Msa0708330:CDS
AAGAGAATGTTGATATCAAA+AGG 0.455565 4_4:-81873284 Msa0708330:CDS
TAGATCAAGAGATTTGGCTT+TGG 0.463035 4_4:+81874252 None:intergenic
GTGAAGTTGCTTAACATGGA+TGG 0.463236 4_4:+81873621 None:intergenic
GTCATATTGTATTGTATGGA+AGG 0.464123 4_4:+81874327 None:intergenic
ATTCAGAAGGTTGGGCTCCA+TGG 0.464333 4_4:-81871830 Msa0708330:CDS
ATTGCAGCTGTTATGGGAGA+TGG 0.472604 4_4:-81872302 Msa0708330:intron
TAACCTTAAGTGCAATATCA+TGG 0.473824 4_4:+81873898 None:intergenic
AGATGATGAAGAAGAATCTA+GGG 0.475990 4_4:+81873932 None:intergenic
GGACATGACCTTTGAGAATA+CGG 0.477411 4_4:-81872267 Msa0708330:CDS
AATGATATTTGGAGATGGAA+TGG 0.477734 4_4:-81873247 Msa0708330:CDS
GAAGGTTGGGCTCCATGGAT+GGG 0.479662 4_4:-81871825 Msa0708330:CDS
GTTTGTGTGCTTCAATGGCT+TGG 0.481327 4_4:+81873868 None:intergenic
AGAAGGTTGGGCTCCATGGA+TGG 0.481727 4_4:-81871826 Msa0708330:CDS
GATGCAGCTGCTAAGCTTAG+TGG 0.483722 4_4:-81873348 Msa0708330:CDS
ACAAGTTTGATGATTGGTAA+TGG 0.484728 4_4:+81873692 None:intergenic
GTTGTTATTCATCTGAAAGC+AGG 0.486478 4_4:-81873315 Msa0708330:CDS
CATAAAGGTATACTAATTTG+TGG 0.487591 4_4:+81874280 None:intergenic
TTCTACCGTTGTGCCTTCAA+AGG 0.490213 4_4:-81872188 Msa0708330:CDS
GTAAGTTTAGGATGAAGAAA+TGG 0.494613 4_4:+81874305 None:intergenic
AATAATAAATGGGAAGAGCT+AGG 0.499818 4_4:+81874151 None:intergenic
GGGAAGTAGTTTAAGTGAGA+GGG 0.501534 4_4:+81873642 None:intergenic
GGTTGATAGTTTATTTGTGG+TGG 0.502880 4_4:+81873953 None:intergenic
AGATTGTTTAGAGCTTTATG+AGG 0.504717 4_4:-81873787 Msa0708330:CDS
CTGCTAAGCTTAGTGGAAAA+GGG 0.504769 4_4:-81873341 Msa0708330:CDS
AGTGGTGTGCCGTTTGATGC+AGG 0.508356 4_4:-81871633 Msa0708330:CDS
ACCTAAATGACAAATTAACT+TGG 0.508748 4_4:-81873725 Msa0708330:CDS
AAGCTTCTTCAGGCAACACC+GGG 0.510144 4_4:-81873450 Msa0708330:CDS
TCAAGAGATTTGGCTTTGGC+GGG 0.510356 4_4:+81874256 None:intergenic
TGCAGTCGCGGTAGAATTGG+CGG 0.511251 4_4:+81872135 None:intergenic
TGAAGTTGCTTAACATGGAT+GGG 0.511299 4_4:+81873622 None:intergenic
TGCTTATGAAATTAAGTGCA+AGG 0.515674 4_4:+81874214 None:intergenic
TGAAATTAAGTGCAAGGGAA+TGG 0.518172 4_4:+81874220 None:intergenic
GTTCTTGAATATTTCTGCCA+TGG 0.518439 4_4:+81871888 None:intergenic
GGTGGAAGAGTTAATTGGCC+TGG 0.520232 4_4:-81871738 Msa0708330:CDS
TAAGACAATATCTGAGGGTG+TGG 0.520252 4_4:-81873370 Msa0708330:CDS
GGGAGATGGTTTCATAGCTA+AGG 0.523196 4_4:-81872288 Msa0708330:CDS
GAAGGCACAACGGTAGAAAA+CGG 0.523739 4_4:+81872193 None:intergenic
TGGTTGCACACATGAGGGTA+TGG 0.524847 4_4:+81873987 None:intergenic
CTTGGTCCTGTCCCTGACCC+CGG 0.525603 4_4:+81873432 None:intergenic
ATTCACAAACCTGCATCAAA+CGG 0.526344 4_4:+81871624 None:intergenic
TATTGCACTTAAGGTTACCT+TGG 0.526896 4_4:-81873892 Msa0708330:CDS
TTTAGTGAAGTTGCTTAACA+TGG 0.527598 4_4:+81873617 None:intergenic
TACCGCGACTGCAACATCTA+CGG 0.528232 4_4:-81872125 Msa0708330:CDS
TTCTTGGTCGTGTTATGTGA+TGG 0.532587 4_4:+81874375 None:intergenic
CGTAAGCATATAAGGTGTCT+TGG 0.533953 4_4:+81872162 None:intergenic
AAGACTTGAAATGATGACTT+TGG 0.535176 4_4:-81871538 Msa0708330:three_prime_UTR
TGAAGTTAGAGTGCTGCTAA+TGG 0.537879 4_4:+81873575 None:intergenic
TGTTATTACTAATCCTTCTG+AGG 0.537907 4_4:-81871709 Msa0708330:CDS
GTGCTGTTACGGTGTTCTGT+TGG 0.539536 4_4:+81872024 None:intergenic
GGTTGGGCTCCATGGATGGG+TGG 0.540114 4_4:-81871822 Msa0708330:CDS
AATTTAATGATATTTGGAGA+TGG 0.540497 4_4:-81873252 Msa0708330:CDS
TATGGTGAATATATGAATGT+TGG 0.547151 4_4:-81871777 Msa0708330:CDS
AAAGCTTCTTCAGGCAACAC+CGG 0.552192 4_4:-81873451 Msa0708330:CDS
GAAAATTTAACAGCCTCAGA+AGG 0.553132 4_4:+81871696 None:intergenic
AATTGAAGTCTATAAAGATA+GGG 0.553959 4_4:-81871493 Msa0708330:three_prime_UTR
GTGGTTGCACAAGATGGAAG+TGG 0.556036 4_4:-81873399 Msa0708330:CDS
CAGAACACCGTAACAGCACA+AGG 0.556039 4_4:-81872020 Msa0708330:CDS
TGGGAAGTAGTTTAAGTGAG+AGG 0.556743 4_4:+81873641 None:intergenic
GCTTATGAAATTAAGTGCAA+GGG 0.558485 4_4:+81874215 None:intergenic
CTTAAGTGCAATATCATGGA+AGG 0.559044 4_4:+81873902 None:intergenic
GATTCTTGGATTTCAGGGAG+TGG 0.559486 4_4:-81871651 Msa0708330:CDS
CGGAGTGATCGGCGCCGGAA+CGG 0.559791 4_4:+81872213 None:intergenic
ATAAAGGTATACTAATTTGT+GGG 0.563799 4_4:+81874281 None:intergenic
AGTAGTTTAAGTGAGAGGGA+AGG 0.567101 4_4:+81873646 None:intergenic
TGTTATGTGATGGGCCATGC+AGG 0.567785 4_4:+81874385 None:intergenic
ACGGTAGAAAACGGAGTGAT+CGG 0.568769 4_4:+81872202 None:intergenic
GGGTCCTGCCGTATTCTCAA+AGG 0.569689 4_4:+81872259 None:intergenic
GTCGCGGTAGAATTGGCGGT+TGG 0.570719 4_4:+81872139 None:intergenic
ATGGATTCTACTATTGTCAC+TGG 0.572496 4_4:-81873228 Msa0708330:CDS
TCTTGGTCGTGTTATGTGAT+GGG 0.573595 4_4:+81874376 None:intergenic
ATATAATGGTTGCACACATG+AGG 0.574017 4_4:+81873981 None:intergenic
AAAGCAAAACCACCCATCCA+TGG 0.574485 4_4:+81871813 None:intergenic
TGCTTCAATGGCTTGGTCCA+AGG 0.576746 4_4:+81873875 None:intergenic
CGGAACGGAGAGCTACTGCT+TGG 0.579824 4_4:+81872228 None:intergenic
GATGGTTTGATAAATTCAGA+AGG 0.580230 4_4:-81871843 Msa0708330:CDS
GAAAACGGAGTGATCGGCGC+CGG 0.581382 4_4:+81872208 None:intergenic
ACAAGCATGCCAAATCAGCA+TGG 0.584779 4_4:-81873812 Msa0708330:CDS
TTCCGTAGATGTTGCAGTCG+CGG 0.586032 4_4:+81872123 None:intergenic
TCAAGTACTAAACAAAATGG+AGG 0.588650 4_4:-81873528 Msa0708330:CDS
GGTGAATATATGAATGTTGG+TGG 0.591996 4_4:-81871774 Msa0708330:CDS
GATATCGTGGTTGCACAAGA+TGG 0.592172 4_4:-81873405 Msa0708330:CDS
ATGACCTTTGAGAATACGGC+AGG 0.599978 4_4:-81872263 Msa0708330:CDS
TTCAGGCAACACCGGGGTCA+GGG 0.601142 4_4:-81873443 Msa0708330:CDS
GATGATGTAGATGCGGATGA+TGG 0.603143 4_4:+81873549 None:intergenic
CAAGCATGCCAAATCAGCAT+GGG 0.604491 4_4:-81873811 Msa0708330:CDS
GAACTATAAGACAATATCTG+AGG 0.605352 4_4:-81873376 Msa0708330:CDS
TATAATGGTTGCACACATGA+GGG 0.607421 4_4:+81873982 None:intergenic
GAAAGTAGCTGAACGAAATG+TGG 0.611952 4_4:+81873179 None:intergenic
AACTATAAGACAATATCTGA+GGG 0.612206 4_4:-81873375 Msa0708330:CDS
CTAAACAAAATGGAGGGAGA+AGG 0.620123 4_4:-81873521 Msa0708330:CDS
GAATATATGAATGTTGGTGG+TGG 0.621736 4_4:-81871771 Msa0708330:CDS
CGGTTTCAAATCATTTGCTG+CGG 0.623680 4_4:+81871944 None:intergenic
CAAGTACTAAACAAAATGGA+GGG 0.628274 4_4:-81873527 Msa0708330:CDS
AGGACCAAGTGCTGATATCG+TGG 0.631260 4_4:-81873418 Msa0708330:CDS
ACAGATCCAAATGAAAACAC+AGG 0.641536 4_4:-81871993 Msa0708330:CDS
TGGTTGCACAAGATGGAAGT+GGG 0.642324 4_4:-81873398 Msa0708330:CDS
AAACATATCTTGGTAGACCA+TGG 0.646826 4_4:-81871905 Msa0708330:CDS
TTTCACGTAAACTTCCTGCA+TGG 0.652686 4_4:-81874399 Msa0708330:five_prime_UTR
GCAACCACGATATCAGCACT+TGG 0.658862 4_4:+81873414 None:intergenic
GGTGGTGGTGCTAATACTGG+TGG 0.661832 4_4:-81871756 Msa0708330:CDS
AGTACTTGATGATGTAGATG+CGG 0.671746 4_4:+81873542 None:intergenic
ATGGCAGAAATATTCAAGAA+CGG 0.671890 4_4:-81871886 Msa0708330:CDS
TTAGTAATAACATGAAAACC+AGG 0.674041 4_4:+81871720 None:intergenic
TGTATAGGAATGAATACGAG+AGG 0.675433 4_4:+81874174 None:intergenic
ACACAAACTTGTATCAACCA+TGG 0.676347 4_4:-81873853 Msa0708330:CDS
GGTAGCCTTTGAAGGCACAA+CGG 0.688392 4_4:+81872183 None:intergenic
AGCTTCTTCAGGCAACACCG+GGG 0.762405 4_4:-81873449 Msa0708330:CDS
AAGTTTACGTGAAATATACG+AGG 0.765155 4_4:+81874408 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTCTATAATAATAAAT+GGG + chr4_4:81871561-81871580 None:intergenic 10.0%
!!! TGTTTTTCTATAATAATAAA+TGG + chr4_4:81871562-81871581 None:intergenic 10.0%
!! AAATATATATATATGATCAA+TGG - chr4_4:81872654-81872673 Msa0708330:intron 10.0%
!! AATATTAATATATGATATCT+TGG + chr4_4:81873035-81873054 None:intergenic 10.0%
!!! TTTGAAATTATTTAATTCCA+TGG + chr4_4:81871866-81871885 None:intergenic 15.0%
!!! ATAAAGGTATACTAATTTGT+GGG + chr4_4:81871421-81871440 None:intergenic 20.0%
!! TATATATAGATCAAGAGATT+TGG + chr4_4:81871456-81871475 None:intergenic 20.0%
!!! CACTATTTCATTTTCTAATA+TGG - chr4_4:81871600-81871619 Msa0708330:three_prime_UTR 20.0%
!! CAGATAATAAATTTCCATAT+TGG - chr4_4:81872208-81872227 Msa0708330:CDS 20.0%
!! GTGACAAATTTAATGATATT+TGG - chr4_4:81872441-81872460 Msa0708330:intron 20.0%
!! AATTTAATGATATTTGGAGA+TGG - chr4_4:81872447-81872466 Msa0708330:intron 20.0%
!!! AATAGATATTGGTTGATTAA+TGG + chr4_4:81872561-81872580 None:intergenic 20.0%
!!! TTTTATTGTTTCTATATGCA+TGG - chr4_4:81872623-81872642 Msa0708330:intron 20.0%
!! AATTCAGTTAAAACATATCT+TGG - chr4_4:81873784-81873803 Msa0708330:CDS 20.0%
!! GTAATGAAAAGTAATATTGA+TGG - chr4_4:81873838-81873857 Msa0708330:CDS 20.0%
!!! ATTTTCTGTTGGTAATTTTT+TGG - chr4_4:81874020-81874039 Msa0708330:CDS 20.0%
!! TAATTGAAGTCTATAAAGAT+AGG - chr4_4:81874205-81874224 Msa0708330:five_prime_UTR 20.0%
!! AATTGAAGTCTATAAAGATA+GGG - chr4_4:81874206-81874225 Msa0708330:five_prime_UTR 20.0%
! AATGGTCATATTGTATTGTA+TGG + chr4_4:81871379-81871398 None:intergenic 25.0%
!! CATAAAGGTATACTAATTTG+TGG + chr4_4:81871422-81871441 None:intergenic 25.0%
! CACAAATTAGTATACCTTTA+TGG - chr4_4:81871420-81871439 Msa0708330:three_prime_UTR 25.0%
! ACCTAAATGACAAATTAACT+TGG - chr4_4:81871974-81871993 Msa0708330:CDS 25.0%
!!! ATTTTGACAAGTTTGATGAT+TGG + chr4_4:81872016-81872035 None:intergenic 25.0%
! AATCATCAAACTTGTCAAAA+TGG - chr4_4:81872015-81872034 Msa0708330:CDS 25.0%
! TCATCAAGTACTAAACAAAA+TGG - chr4_4:81872168-81872187 Msa0708330:CDS 25.0%
! AACTATAAGACAATATCTGA+GGG - chr4_4:81872324-81872343 Msa0708330:intron 25.0%
! AAGAGAATGTTGATATCAAA+AGG - chr4_4:81872415-81872434 Msa0708330:intron 25.0%
! AAGTAACGTTTGAGAAATAA+AGG + chr4_4:81872907-81872926 None:intergenic 25.0%
! TTAACAAACTTTCTCACAAA+AGG - chr4_4:81872946-81872965 Msa0708330:intron 25.0%
! AATAATTATTGCAGCTGTTA+TGG - chr4_4:81873390-81873409 Msa0708330:CDS 25.0%
! ATAATTATTGCAGCTGTTAT+GGG - chr4_4:81873391-81873410 Msa0708330:CDS 25.0%
! TTTGATAAATTCAGAAGGTT+GGG - chr4_4:81873861-81873880 Msa0708330:CDS 25.0%
! TATGGTGAATATATGAATGT+TGG - chr4_4:81873922-81873941 Msa0708330:CDS 25.0%
! TTAGTAATAACATGAAAACC+AGG + chr4_4:81873982-81874001 None:intergenic 25.0%
!! TATTGTCATGTAGTTTTTGT+TGG - chr4_4:81874233-81874252 Msa0708330:five_prime_UTR 25.0%
!! ATTGTCATGTAGTTTTTGTT+GGG - chr4_4:81874234-81874253 Msa0708330:five_prime_UTR 25.0%
!! TCTTGTATAACTTTTGTACA+AGG + chr4_4:81874265-81874284 None:intergenic 25.0%
CTAACATTTCACAAAGTTCT+TGG + chr4_4:81871343-81871362 None:intergenic 30.0%
GTCATATTGTATTGTATGGA+AGG + chr4_4:81871375-81871394 None:intergenic 30.0%
! GTAAGTTTAGGATGAAGAAA+TGG + chr4_4:81871397-81871416 None:intergenic 30.0%
! TAATTTGTGGGTGTAAGTTT+AGG + chr4_4:81871409-81871428 None:intergenic 30.0%
GCTTATGAAATTAAGTGCAA+GGG + chr4_4:81871487-81871506 None:intergenic 30.0%
TGCTTATGAAATTAAGTGCA+AGG + chr4_4:81871488-81871507 None:intergenic 30.0%
AATAATAAATGGGAAGAGCT+AGG + chr4_4:81871551-81871570 None:intergenic 30.0%
!! TAGGGTTGATAGTTTATTTG+TGG + chr4_4:81871752-81871771 None:intergenic 30.0%
AGATGATGAAGAAGAATCTA+GGG + chr4_4:81871770-81871789 None:intergenic 30.0%
AAGATGATGAAGAAGAATCT+AGG + chr4_4:81871771-81871790 None:intergenic 30.0%
TAACCTTAAGTGCAATATCA+TGG + chr4_4:81871804-81871823 None:intergenic 30.0%
! AGATTGTTTAGAGCTTTATG+AGG - chr4_4:81871912-81871931 Msa0708330:CDS 30.0%
!! GCCAAGTTAATTTGTCATTT+AGG + chr4_4:81871978-81871997 None:intergenic 30.0%
! ACAAGTTTGATGATTGGTAA+TGG + chr4_4:81872010-81872029 None:intergenic 30.0%
TTTAGTGAAGTTGCTTAACA+TGG + chr4_4:81872085-81872104 None:intergenic 30.0%
TCAAGTACTAAACAAAATGG+AGG - chr4_4:81872171-81872190 Msa0708330:CDS 30.0%
CAAGTACTAAACAAAATGGA+GGG - chr4_4:81872172-81872191 Msa0708330:CDS 30.0%
!! AAATTTCCATATTGGCTTTC+AGG - chr4_4:81872216-81872235 Msa0708330:CDS 30.0%
!! AATTTCCATATTGGCTTTCA+GGG - chr4_4:81872217-81872236 Msa0708330:CDS 30.0%
GAACTATAAGACAATATCTG+AGG - chr4_4:81872323-81872342 Msa0708330:intron 30.0%
AATGATATTTGGAGATGGAA+TGG - chr4_4:81872452-81872471 Msa0708330:intron 30.0%
GGTAATCAAAATGCTATAGA+TGG - chr4_4:81872492-81872511 Msa0708330:intron 30.0%
AGAGCTACATGAATAGATAT+TGG + chr4_4:81872572-81872591 None:intergenic 30.0%
! GTTTTGTACAAACTGTGTTA+CGG - chr4_4:81872767-81872786 Msa0708330:intron 30.0%
! ACAAACAGTGTGAAACTTTT+AGG + chr4_4:81873261-81873280 None:intergenic 30.0%
!! GGAACTATTGATTTCATCTT+CGG - chr4_4:81873595-81873614 Msa0708330:CDS 30.0%
! ACAATTCCTGTGTTTTCATT+TGG + chr4_4:81873715-81873734 None:intergenic 30.0%
!!! GTTTTAACTGAATTCTGAAC+CGG + chr4_4:81873778-81873797 None:intergenic 30.0%
ATGGCAGAAATATTCAAGAA+CGG - chr4_4:81873813-81873832 Msa0708330:CDS 30.0%
GATGGTTTGATAAATTCAGA+AGG - chr4_4:81873856-81873875 Msa0708330:CDS 30.0%
GTTTGATAAATTCAGAAGGT+TGG - chr4_4:81873860-81873879 Msa0708330:CDS 30.0%
! GCTTTGAGTACACTTTATTA+TGG - chr4_4:81873904-81873923 Msa0708330:CDS 30.0%
TGTTATTACTAATCCTTCTG+AGG - chr4_4:81873990-81874009 Msa0708330:CDS 30.0%
TGAGATGATGTATAGTATAG+TGG - chr4_4:81874136-81874155 Msa0708330:five_prime_UTR 30.0%
AAGACTTGAAATGATGACTT+TGG - chr4_4:81874161-81874180 Msa0708330:five_prime_UTR 30.0%
TCCATTTCCTTTACTCAAAA+GGG - chr4_4:81874301-81874320 Msa0708330:five_prime_UTR 30.0%
!! TATTTGACCCTTTTGAGTAA+AGG + chr4_4:81874311-81874330 None:intergenic 30.0%
! TAGATCAAGAGATTTGGCTT+TGG + chr4_4:81871450-81871469 None:intergenic 35.0%
TGAAATTAAGTGCAAGGGAA+TGG + chr4_4:81871482-81871501 None:intergenic 35.0%
TGTATAGGAATGAATACGAG+AGG + chr4_4:81871528-81871547 None:intergenic 35.0%
TATAATGGTTGCACACATGA+GGG + chr4_4:81871720-81871739 None:intergenic 35.0%
ATATAATGGTTGCACACATG+AGG + chr4_4:81871721-81871740 None:intergenic 35.0%
CATGTGTGCAACCATTATAT+TGG - chr4_4:81871721-81871740 Msa0708330:CDS 35.0%
TTGTGGTGGTTCCAATATAA+TGG + chr4_4:81871735-81871754 None:intergenic 35.0%
!! GGTTGATAGTTTATTTGTGG+TGG + chr4_4:81871749-81871768 None:intergenic 35.0%
CTTAAGTGCAATATCATGGA+AGG + chr4_4:81871800-81871819 None:intergenic 35.0%
CTTCCATGATATTGCACTTA+AGG - chr4_4:81871798-81871817 Msa0708330:CDS 35.0%
TATTGCACTTAAGGTTACCT+TGG - chr4_4:81871807-81871826 Msa0708330:CDS 35.0%
TACAAGTTTGTGTGCTTCAA+TGG + chr4_4:81871839-81871858 None:intergenic 35.0%
ACACAAACTTGTATCAACCA+TGG - chr4_4:81871846-81871865 Msa0708330:CDS 35.0%
TGAAGTTGCTTAACATGGAT+GGG + chr4_4:81872080-81872099 None:intergenic 35.0%
!! AGTACTTGATGATGTAGATG+CGG + chr4_4:81872160-81872179 None:intergenic 35.0%
! GTTGTTATTCATCTGAAAGC+AGG - chr4_4:81872384-81872403 Msa0708330:intron 35.0%
ATGGATTCTACTATTGTCAC+TGG - chr4_4:81872471-81872490 Msa0708330:intron 35.0%
! ACATTTCGTTCAGCTACTTT+CGG - chr4_4:81872522-81872541 Msa0708330:intron 35.0%
! ACTTTTGCCCCATTAAGATA+GGG - chr4_4:81872730-81872749 Msa0708330:intron 35.0%
AAACGTTACTTAGCAACTCA+TGG - chr4_4:81872916-81872935 Msa0708330:intron 35.0%
TACATCAGTCTCACCAATTA+TGG - chr4_4:81873072-81873091 Msa0708330:intron 35.0%
GATACCTTAGATGCCATAAT+TGG + chr4_4:81873088-81873107 None:intergenic 35.0%
TCACACTGTTTGTACATCAT+TGG - chr4_4:81873268-81873287 Msa0708330:CDS 35.0%
ACAGATCCAAATGAAAACAC+AGG - chr4_4:81873706-81873725 Msa0708330:CDS 35.0%
! AATCATTTGCTGCGGTTATA+CGG + chr4_4:81873750-81873769 None:intergenic 35.0%
AAACATATCTTGGTAGACCA+TGG - chr4_4:81873794-81873813 Msa0708330:CDS 35.0%
! GTTCTTGAATATTTCTGCCA+TGG + chr4_4:81873814-81873833 None:intergenic 35.0%
GGTGAATATATGAATGTTGG+TGG - chr4_4:81873925-81873944 Msa0708330:CDS 35.0%
! GAATATATGAATGTTGGTGG+TGG - chr4_4:81873928-81873947 Msa0708330:CDS 35.0%
ATACTGGTGGAAGAGTTAAT+TGG - chr4_4:81873956-81873975 Msa0708330:CDS 35.0%
GAAAATTTAACAGCCTCAGA+AGG + chr4_4:81874006-81874025 None:intergenic 35.0%
! GAGGCTGTTAAATTTTCTGT+TGG - chr4_4:81874009-81874028 Msa0708330:CDS 35.0%
!!! TCTGTTGGTAATTTTTTGGC+TGG - chr4_4:81874024-81874043 Msa0708330:CDS 35.0%
!!! ATTTTTTGGCTGGTGATTCT+TGG - chr4_4:81874034-81874053 Msa0708330:CDS 35.0%
ATTCACAAACCTGCATCAAA+CGG + chr4_4:81874078-81874097 None:intergenic 35.0%
! GACTTTGGTGAAAGCATATA+TGG - chr4_4:81874176-81874195 Msa0708330:five_prime_UTR 35.0%
GTCCATTTCCTTTACTCAAA+AGG - chr4_4:81874300-81874319 Msa0708330:five_prime_UTR 35.0%
!! ACCCTTTTGAGTAAAGGAAA+TGG + chr4_4:81874305-81874324 None:intergenic 35.0%
TTTCACGTAAACTTCCTGCA+TGG - chr4_4:81871300-81871319 Msa0708330:three_prime_UTR 40.0%
TCTTGGTCGTGTTATGTGAT+GGG + chr4_4:81871326-81871345 None:intergenic 40.0%
TTCTTGGTCGTGTTATGTGA+TGG + chr4_4:81871327-81871346 None:intergenic 40.0%
!! ATCAAGAGATTTGGCTTTGG+CGG + chr4_4:81871447-81871466 None:intergenic 40.0%
! TTCACTCTCTTGTTTGTGCA+TGG - chr4_4:81871664-81871683 Msa0708330:CDS 40.0%
! ACAATCTTCCCATGCTGATT+TGG + chr4_4:81871899-81871918 None:intergenic 40.0%
AGTAGTTTAAGTGAGAGGGA+AGG + chr4_4:81872056-81872075 None:intergenic 40.0%
GGGAAGTAGTTTAAGTGAGA+GGG + chr4_4:81872060-81872079 None:intergenic 40.0%
TGGGAAGTAGTTTAAGTGAG+AGG + chr4_4:81872061-81872080 None:intergenic 40.0%
GTGAAGTTGCTTAACATGGA+TGG + chr4_4:81872081-81872100 None:intergenic 40.0%
!! TGAAGTTAGAGTGCTGCTAA+TGG + chr4_4:81872127-81872146 None:intergenic 40.0%
CTAAACAAAATGGAGGGAGA+AGG - chr4_4:81872178-81872197 Msa0708330:CDS 40.0%
GTCAGATAGAAAGCTTCTTC+AGG - chr4_4:81872239-81872258 Msa0708330:CDS 40.0%
TAAGACAATATCTGAGGGTG+TGG - chr4_4:81872329-81872348 Msa0708330:intron 40.0%
CTGCTAAGCTTAGTGGAAAA+GGG - chr4_4:81872358-81872377 Msa0708330:intron 40.0%
GAAAGTAGCTGAACGAAATG+TGG + chr4_4:81872523-81872542 None:intergenic 40.0%
! CACTTTTGCCCCATTAAGAT+AGG - chr4_4:81872729-81872748 Msa0708330:intron 40.0%
TGCATGTCCCTATCTTAATG+GGG + chr4_4:81872740-81872759 None:intergenic 40.0%
CTGCATGTCCCTATCTTAAT+GGG + chr4_4:81872741-81872760 None:intergenic 40.0%
ACTGCATGTCCCTATCTTAA+TGG + chr4_4:81872742-81872761 None:intergenic 40.0%
CTCACCAATTATGGCATCTA+AGG - chr4_4:81873081-81873100 Msa0708330:intron 40.0%
! GGACATGACCTTTGAGAATA+CGG - chr4_4:81873432-81873451 Msa0708330:CDS 40.0%
!!! AGCTACTGCTTGGTGTTTTT+GGG + chr4_4:81873464-81873483 None:intergenic 40.0%
! CGTAAGCATATAAGGTGTCT+TGG + chr4_4:81873540-81873559 None:intergenic 40.0%
!! CGAAGATGTTGCAGTTTTGA+AGG + chr4_4:81873636-81873655 None:intergenic 40.0%
! TGTTCTTCCTTGTGCTGTTA+CGG + chr4_4:81873689-81873708 None:intergenic 40.0%
! CGGTTTCAAATCATTTGCTG+CGG + chr4_4:81873758-81873777 None:intergenic 40.0%
! CGCAGCAAATGATTTGAAAC+CGG - chr4_4:81873756-81873775 Msa0708330:CDS 40.0%
CTGGTGATTCTTGGATTTCA+GGG - chr4_4:81874043-81874062 Msa0708330:CDS 40.0%
!! TCAAGAGATTTGGCTTTGGC+GGG + chr4_4:81871446-81871465 None:intergenic 45.0%
ATGGGAAGAGCTAGGTGTAT+AGG + chr4_4:81871543-81871562 None:intergenic 45.0%
GTTTGTGTGCTTCAATGGCT+TGG + chr4_4:81871834-81871853 None:intergenic 45.0%
ACAAGCATGCCAAATCAGCA+TGG - chr4_4:81871887-81871906 Msa0708330:CDS 45.0%
CAAGCATGCCAAATCAGCAT+GGG - chr4_4:81871888-81871907 Msa0708330:CDS 45.0%
GATGATGTAGATGCGGATGA+TGG + chr4_4:81872153-81872172 None:intergenic 45.0%
TCTGACCCTGAAAGCCAATA+TGG + chr4_4:81872225-81872244 None:intergenic 45.0%
AAAGCTTCTTCAGGCAACAC+CGG - chr4_4:81872248-81872267 Msa0708330:CDS 45.0%
GATATCGTGGTTGCACAAGA+TGG - chr4_4:81872294-81872313 Msa0708330:CDS 45.0%
TGGTTGCACAAGATGGAAGT+GGG - chr4_4:81872301-81872320 Msa0708330:intron 45.0%
GCTGCTAAGCTTAGTGGAAA+AGG - chr4_4:81872357-81872376 Msa0708330:intron 45.0%
ATTGCAGCTGTTATGGGAGA+TGG - chr4_4:81873397-81873416 Msa0708330:CDS 45.0%
GGGAGATGGTTTCATAGCTA+AGG - chr4_4:81873411-81873430 Msa0708330:CDS 45.0%
! ATGACCTTTGAGAATACGGC+AGG - chr4_4:81873436-81873455 Msa0708330:CDS 45.0%
!!! GCTACTGCTTGGTGTTTTTG+GGG + chr4_4:81873463-81873482 None:intergenic 45.0%
!!! GAGCTACTGCTTGGTGTTTT+TGG + chr4_4:81873465-81873484 None:intergenic 45.0%
ACGGTAGAAAACGGAGTGAT+CGG + chr4_4:81873500-81873519 None:intergenic 45.0%
! GAAGGCACAACGGTAGAAAA+CGG + chr4_4:81873509-81873528 None:intergenic 45.0%
TTCTACCGTTGTGCCTTCAA+AGG - chr4_4:81873511-81873530 Msa0708330:CDS 45.0%
! GTGTTCTGTTGGTTGCTCAT+TGG + chr4_4:81873667-81873686 None:intergenic 45.0%
AAAGCAAAACCACCCATCCA+TGG + chr4_4:81873889-81873908 None:intergenic 45.0%
GCTGGTGATTCTTGGATTTC+AGG - chr4_4:81874042-81874061 Msa0708330:CDS 45.0%
GATTCTTGGATTTCAGGGAG+TGG - chr4_4:81874048-81874067 Msa0708330:CDS 45.0%
!! TGTTATGTGATGGGCCATGC+AGG + chr4_4:81871317-81871336 None:intergenic 50.0%
TGGTTGCACACATGAGGGTA+TGG + chr4_4:81871715-81871734 None:intergenic 50.0%
!! TGCTTCAATGGCTTGGTCCA+AGG + chr4_4:81871827-81871846 None:intergenic 50.0%
AAGCTTCTTCAGGCAACACC+GGG - chr4_4:81872249-81872268 Msa0708330:CDS 50.0%
!! AGGACCAAGTGCTGATATCG+TGG - chr4_4:81872281-81872300 Msa0708330:CDS 50.0%
GCAACCACGATATCAGCACT+TGG + chr4_4:81872288-81872307 None:intergenic 50.0%
GTGGTTGCACAAGATGGAAG+TGG - chr4_4:81872300-81872319 Msa0708330:intron 50.0%
GATGCAGCTGCTAAGCTTAG+TGG - chr4_4:81872351-81872370 Msa0708330:intron 50.0%
! GGTAGCCTTTGAAGGCACAA+CGG + chr4_4:81873519-81873538 None:intergenic 50.0%
! GGTGTCTTGGTAGCCTTTGA+AGG + chr4_4:81873527-81873546 None:intergenic 50.0%
GCGGTTGGCGTAAGCATATA+AGG + chr4_4:81873548-81873567 None:intergenic 50.0%
TGTTGCAGTCGCGGTAGAAT+TGG + chr4_4:81873570-81873589 None:intergenic 50.0%
TACCGCGACTGCAACATCTA+CGG - chr4_4:81873574-81873593 Msa0708330:CDS 50.0%
TTCCGTAGATGTTGCAGTCG+CGG + chr4_4:81873579-81873598 None:intergenic 50.0%
! GTGCTGTTACGGTGTTCTGT+TGG + chr4_4:81873678-81873697 None:intergenic 50.0%
CAGAACACCGTAACAGCACA+AGG - chr4_4:81873679-81873698 Msa0708330:CDS 50.0%
ATTCAGAAGGTTGGGCTCCA+TGG - chr4_4:81873869-81873888 Msa0708330:CDS 50.0%
!! GTTGGTGGTGGTGCTAATAC+TGG - chr4_4:81873940-81873959 Msa0708330:CDS 50.0%
GGTGGAAGAGTTAATTGGCC+TGG - chr4_4:81873961-81873980 Msa0708330:CDS 50.0%
!! TTGGCTTTGGCGGGCCATAA+AGG + chr4_4:81871437-81871456 None:intergenic 55.0%
AGCTTCTTCAGGCAACACCG+GGG - chr4_4:81872250-81872269 Msa0708330:CDS 55.0%
GGGTCCTGCCGTATTCTCAA+AGG + chr4_4:81873443-81873462 None:intergenic 55.0%
CAAGCAGTAGCTCTCCGTTC+CGG - chr4_4:81873472-81873491 Msa0708330:CDS 55.0%
TGCAGTCGCGGTAGAATTGG+CGG + chr4_4:81873567-81873586 None:intergenic 55.0%
AGAAGGTTGGGCTCCATGGA+TGG - chr4_4:81873873-81873892 Msa0708330:CDS 55.0%
GAAGGTTGGGCTCCATGGAT+GGG - chr4_4:81873874-81873893 Msa0708330:CDS 55.0%
! GGTGGTGGTGCTAATACTGG+TGG - chr4_4:81873943-81873962 Msa0708330:CDS 55.0%
!! AGTGGTGTGCCGTTTGATGC+AGG - chr4_4:81874066-81874085 Msa0708330:CDS 55.0%
TTCAGGCAACACCGGGGTCA+GGG - chr4_4:81872256-81872275 Msa0708330:CDS 60.0%
CGGAACGGAGAGCTACTGCT+TGG + chr4_4:81873474-81873493 None:intergenic 60.0%
GAAAACGGAGTGATCGGCGC+CGG + chr4_4:81873494-81873513 None:intergenic 60.0%
GTCGCGGTAGAATTGGCGGT+TGG + chr4_4:81873563-81873582 None:intergenic 60.0%
CTTCAGGCAACACCGGGGTC+AGG - chr4_4:81872255-81872274 Msa0708330:CDS 65.0%
CTTGGTCCTGTCCCTGACCC+CGG + chr4_4:81872270-81872289 None:intergenic 65.0%
GGTTGGGCTCCATGGATGGG+TGG - chr4_4:81873877-81873896 Msa0708330:CDS 65.0%
GCAACACCGGGGTCAGGGAC+AGG - chr4_4:81872261-81872280 Msa0708330:CDS 70.0%
! CGGAGTGATCGGCGCCGGAA+CGG + chr4_4:81873489-81873508 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 81871292 81874429 81871292 ID=Msa0708330;Name=Msa0708330
chr4_4 mRNA 81871292 81874429 81871292 ID=Msa0708330-mRNA-1;Parent=Msa0708330;Name=Msa0708330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=303|1|1|1|1|1|2|334|546
chr4_4 exon 81871292 81872317 81871292 ID=Msa0708330-mRNA-1:exon:26906;Parent=Msa0708330-mRNA-1
chr4_4 exon 81873178 81874429 81873178 ID=Msa0708330-mRNA-1:exon:26905;Parent=Msa0708330-mRNA-1
chr4_4 five_prime_UTR 81874127 81874429 81874127 ID=Msa0708330-mRNA-1:five_prime_utr;Parent=Msa0708330-mRNA-1
chr4_4 CDS 81873178 81874126 81873178 ID=Msa0708330-mRNA-1:cds;Parent=Msa0708330-mRNA-1
chr4_4 CDS 81871626 81872317 81871626 ID=Msa0708330-mRNA-1:cds;Parent=Msa0708330-mRNA-1
chr4_4 three_prime_UTR 81871292 81871625 81871292 ID=Msa0708330-mRNA-1:three_prime_utr;Parent=Msa0708330-mRNA-1
Gene Sequence

>Msa0708330

ATGCACACTATTTCATTTTCTAATATGGATAAACTCATATTCAACTCTTGCATTGCATACTTTCTACTTTTCACTCTCTTGTTTGTGCATGGCAAAGAATTGTCATGCAATCAAACTCCATACCCTCATGTGTGCAACCATTATATTGGAACCACCACAAATAAACTATCAACCCTAGATTCTTCTTCATCATCTTCTTCTTCCTTCCATGATATTGCACTTAAGGTTACCTTGGACCAAGCCATTGAAGCACACAAACTTGTATCAACCATGGAATTAAATAATTTCAAAGACAAGCATGCCAAATCAGCATGGGAAGATTGTTTAGAGCTTTATGAGGATACAATATATCAACTCAAACGCTCAATAAACTCAAACAACCTAAATGACAAATTAACTTGGCAAAGTACTTCCATTACCAATCATCAAACTTGTCAAAATGGTTTCATTGATTTCAACCTTCCCTCTCACTTAAACTACTTCCCATCCATGTTAAGCAACTTCACTAAATTGCTTAGTAATTCTTTGTCCATTAGCAGCACTCTAACTTCATCACCATCATCCGCATCTACATCATCAAGTACTAAACAAAATGGAGGGAGAAGGTTGTTATCAGATAATAAATTTCCATATTGGCTTTCAGGGTCAGATAGAAAGCTTCTTCAGGCAACACCGGGGTCAGGGACAGGACCAAGTGCTGATATCGTGGTTGCACAAGATGGAAGTGGGAACTATAAGACAATATCTGAGGGTGTGGATGCAGCTGCTAAGCTTAGTGGAAAAGGGAGAGTTGTTATTCATCTGAAAGCAGGTGTTTATAAAGAGAATGTTGATATCAAAAGGACAGTGACAAATTTAATGATATTTGGAGATGGAATGGATTCTACTATTGTCACTGGTAATCAAAATGCTATAGATGGTTCTACCACATTTCGTTCAGCTACTTTCGCTGTTATGGGAGATGGTTTCATAGCTAAGGACATGACCTTTGAGAATACGGCAGGACCCCAAAAACACCAAGCAGTAGCTCTCCGTTCCGGCGCCGATCACTCCGTTTTCTACCGTTGTGCCTTCAAAGGCTACCAAGACACCTTATATGCTTACGCCAACCGCCAATTCTACCGCGACTGCAACATCTACGGAACTATTGATTTCATCTTCGGCAACGCAGTTACTGTCCTTCAAAACTGCAACATCTTCGTTAGAAAACCAATGAGCAACCAACAGAACACCGTAACAGCACAAGGAAGAACAGATCCAAATGAAAACACAGGAATTGTAATACATAACTGCCGTATAACCGCAGCAAATGATTTGAAACCGGTTCAGAATTCAGTTAAAACATATCTTGGTAGACCATGGCAGAAATATTCAAGAACGGTTGTAATGAAAAGTAATATTGATGGTTTGATAAATTCAGAAGGTTGGGCTCCATGGATGGGTGGTTTTGCTTTGAGTACACTTTATTATGGTGAATATATGAATGTTGGTGGTGGTGCTAATACTGGTGGAAGAGTTAATTGGCCTGGTTTTCATGTTATTACTAATCCTTCTGAGGCTGTTAAATTTTCTGTTGGTAATTTTTTGGCTGGTGATTCTTGGATTTCAGGGAGTGGTGTGCCGTTTGATGCAGGTTTGTGA
Protein sequence

>Msa0708330

MHTISFSNMDKLIFNSCIAYFLLFTLLFVHGKELSCNQTPYPHVCNHYIGTTTNKLSTLDSSSSSSSSFHDIALKVTLDQAIEAHKLVSTMELNNFKDKHAKSAWEDCLELYEDTIYQLKRSINSNNLNDKLTWQSTSITNHQTCQNGFIDFNLPSHLNYFPSMLSNFTKLLSNSLSISSTLTSSPSSASTSSSTKQNGGRRLLSDNKFPYWLSGSDRKLLQATPGSGTGPSADIVVAQDGSGNYKTISEGVDAAAKLSGKGRVVIHLKAGVYKENVDIKRTVTNLMIFGDGMDSTIVTGNQNAIDGSTTFRSATFAVMGDGFIAKDMTFENTAGPQKHQAVALRSGADHSVFYRCAFKGYQDTLYAYANRQFYRDCNIYGTIDFIFGNAVTVLQNCNIFVRKPMSNQQNTVTAQGRTDPNENTGIVIHNCRITAANDLKPVQNSVKTYLGRPWQKYSRTVVMKSNIDGLINSEGWAPWMGGFALSTLYYGEYMNVGGGANTGGRVNWPGFHVITNPSEAVKFSVGNFLAGDSWISGSGVPFDAGL*