AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0575330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575330 MtrunA17_Chr8g0392471 95.213 188 9 0 52 239 95 282 4.45e-120 343
Msa0575330 MtrunA17_Chr8g0392471 95.918 98 4 0 154 251 1 98 2.57e-64 202
Msa0575330 MtrunA17_Chr8g0392461 68.103 232 66 3 5 231 4 232 3.26e-110 317
Msa0575330 MtrunA17_Chr5g0428761 53.211 218 89 4 3 208 2 218 1.81e-70 216
Msa0575330 MtrunA17_Chr8g0392481 94.624 93 5 0 1 93 1 93 2.73e-59 183
Msa0575330 MtrunA17_Chr1g0188581 37.915 211 119 4 6 206 7 215 4.86e-38 133
Msa0575330 MtrunA17_Chr1g0147511 36.232 207 126 1 6 206 7 213 7.92e-38 132
Msa0575330 MtrunA17_Chr3g0139261 36.232 207 126 2 6 206 7 213 8.52e-37 129
Msa0575330 MtrunA17_Chr6g0477471 34.300 207 130 2 6 206 7 213 9.01e-32 116
Msa0575330 MtrunA17_Chr6g0477681 34.135 208 129 5 6 206 7 213 7.42e-25 98.6
Msa0575330 MtrunA17_Chr6g0477661 30.918 207 137 3 6 206 7 213 9.33e-25 98.2
Msa0575330 MtrunA17_Chr2g0282971 31.220 205 119 5 39 232 1 194 8.13e-24 95.1
Msa0575330 MtrunA17_Chr7g0244961 28.846 208 119 5 1 192 38 232 1.18e-15 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575330 AT3G18215.1 58.607 244 95 1 1 238 1 244 2.51e-96 282
Msa0575330 AT5G24600.1 53.153 222 88 3 3 208 2 223 1.09e-70 217
Msa0575330 AT5G24600.2 53.153 222 88 3 3 208 2 223 1.30e-70 217
Msa0575330 AT3G18215.2 57.558 172 67 1 73 238 1 172 2.08e-61 191
Msa0575330 AT5G46060.1 37.931 232 127 4 3 224 6 230 1.59e-33 122
Msa0575330 AT4G31330.1 35.616 219 128 5 1 206 1 219 1.57e-32 119
Msa0575330 AT5G10580.1 32.114 246 147 5 6 237 7 246 3.86e-32 118
Msa0575330 AT5G10580.4 32.114 246 147 5 6 237 7 246 3.86e-32 118
Msa0575330 AT5G10580.3 32.377 244 143 5 6 235 7 242 7.68e-32 117
Msa0575330 AT5G24790.1 29.583 240 156 4 6 232 7 246 1.04e-24 99.0
Msa0575330 AT5G10580.2 36.986 146 86 2 6 145 7 152 6.48e-22 90.1
Msa0575330 AT5G43180.1 25.751 233 140 5 8 212 8 235 5.40e-13 67.0

Find 62 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTCTTCCTATGGATTTA+TGG 0.078037 4_1:-79191374 Msa0575330:CDS
CTCTCTTCCTATGGATTTAT+GGG 0.158265 4_1:-79191373 Msa0575330:CDS
AATCCAAAGTAGTAATTATT+TGG 0.193942 4_1:-79192704 Msa0575330:five_prime_UTR
AATAATTACTACTTTGGATT+TGG 0.216277 4_1:+79192707 None:intergenic
TTTAATGAGGTTGATGTCTT+TGG 0.223912 4_1:-79192838 Msa0575330:five_prime_UTR
ATCACACTAAGTTCACTTAT+TGG 0.238986 4_1:-79191665 Msa0575330:CDS
GTTTGTACCATTGGGATTAT+TGG 0.289538 4_1:-79192648 Msa0575330:CDS
ATTCGTAGCAATTACAGTTT+TGG 0.290029 4_1:-79191143 Msa0575330:three_prime_UTR
TGAAGAAGTACTTTGAAGTA+TGG 0.301496 4_1:+79191601 None:intergenic
TCCCTTCAAGAACGGTGTTT+TGG 0.320103 4_1:-79191744 Msa0575330:CDS
ACCTACACTCAGAGATAAAA+AGG 0.325592 4_1:-79191480 Msa0575330:CDS
TGATATTGTTGCGGATAGTT+TGG 0.326815 4_1:+79191717 None:intergenic
CCACCAAATAATTACTACTT+TGG 0.332002 4_1:+79192701 None:intergenic
TTCAATAAGAGTAGAGTTCT+AGG 0.334940 4_1:-79191052 Msa0575330:three_prime_UTR
GTCAGTGTCACTCTCATGTT+TGG 0.336541 4_1:-79192862 Msa0575330:five_prime_UTR
CTTGATAGTACAAGATTCTA+TGG 0.353262 4_1:+79192751 None:intergenic
AGGGTTGTCGAATTCATTTA+AGG 0.363639 4_1:-79191006 Msa0575330:three_prime_UTR
GGTGAATCTTCCATTTCAAT+TGG 0.367905 4_1:-79192899 Msa0575330:five_prime_UTR
TTAATGAGGTTGATGTCTTT+GGG 0.372553 4_1:-79192837 Msa0575330:five_prime_UTR
TATCATTTCCTCTCTTCCTA+TGG 0.386632 4_1:-79191382 Msa0575330:CDS
GCAAGTTGCAACTTGTTGAT+AGG 0.412995 4_1:+79192981 None:intergenic
GAACCTCATCGAACTGTCAT+TGG 0.422795 4_1:-79192581 Msa0575330:CDS
GATGGAGAACAACGAGCATC+TGG 0.424053 4_1:-79192678 Msa0575330:CDS
ACGCTGAGTCACGTATTCAA+TGG 0.435962 4_1:-79192553 Msa0575330:CDS
GCTAGTCACTCTTGGTCCCT+TGG 0.438401 4_1:-79191422 Msa0575330:CDS
GCTAAATTGTTACCAGCTAA+AGG 0.439017 4_1:+79191203 None:intergenic
GCTGATTATTATCCTTTAGC+TGG 0.444834 4_1:-79191215 Msa0575330:CDS
GGAGTATGTGTTTGTACCAT+TGG 0.450989 4_1:-79192657 Msa0575330:CDS
CGCTGAGTCACGTATTCAAT+GGG 0.461213 4_1:-79192552 Msa0575330:CDS
CGCCAAAACACCGTTCTTGA+AGG 0.462146 4_1:+79191742 None:intergenic
CAACAAGCAAACATTGGTAT+AGG 0.462578 4_1:+79191350 None:intergenic
GAGTATGTGTTTGTACCATT+GGG 0.476547 4_1:-79192656 Msa0575330:CDS
AGTAAAATGCTCGCAATCCA+AGG 0.482507 4_1:+79191405 None:intergenic
TTTGGGTTGATCTCATTACT+AGG 0.487139 4_1:-79192820 Msa0575330:five_prime_UTR
GTAGTAATTATTTGGTGGGA+TGG 0.488750 4_1:-79192696 Msa0575330:exon
GAGGTACAACACACATGAAT+GGG 0.493426 4_1:+79190959 None:intergenic
CCAAAGTAGTAATTATTTGG+TGG 0.499862 4_1:-79192701 Msa0575330:five_prime_UTR
ATTCATGTGTGTTGTACCTC+TGG 0.499923 4_1:-79190956 Msa0575330:three_prime_UTR
TATGAACATGTGCCTTGGAT+TGG 0.508171 4_1:-79191087 Msa0575330:three_prime_UTR
AGAGGTACAACACACATGAA+TGG 0.508371 4_1:+79190958 None:intergenic
CAAAGTAGTAATTATTTGGT+GGG 0.530732 4_1:-79192700 Msa0575330:five_prime_UTR
TGAATCGCGCTAGTCACTCT+TGG 0.544487 4_1:-79191430 Msa0575330:CDS
AACTATCCGCAACAATATCA+TGG 0.557822 4_1:-79191714 Msa0575330:CDS
ATGTGCCTTGGATTGGATGT+TGG 0.562422 4_1:-79191080 Msa0575330:three_prime_UTR
AAGTATATGAACATGTGCCT+TGG 0.566367 4_1:-79191092 Msa0575330:three_prime_UTR
TGAGTGACTCACACTCATCA+TGG 0.574492 4_1:+79192523 None:intergenic
TTTCAGGATCCCTTCAAGAA+CGG 0.574856 4_1:-79191752 Msa0575330:intron
GTATAGGCCCATAAATCCAT+AGG 0.583485 4_1:+79191366 None:intergenic
GCCAAAACACCGTTCTTGAA+GGG 0.587893 4_1:+79191743 None:intergenic
TCATAGTGACTCATTCAGAA+AGG 0.595692 4_1:-79191267 Msa0575330:CDS
CCATAAATCCATAGGAAGAG+AGG 0.597591 4_1:+79191374 None:intergenic
TAGACGCCATGATATTGTTG+CGG 0.610202 4_1:+79191708 None:intergenic
AAGCAGCAACAAGCAAACAT+TGG 0.610513 4_1:+79191344 None:intergenic
TCATTCCAACATCCAATCCA+AGG 0.617086 4_1:+79191075 None:intergenic
GTAAAATGCTCGCAATCCAA+GGG 0.634647 4_1:+79191406 None:intergenic
AAGAACACCAATAATCCCAA+TGG 0.652121 4_1:+79192641 None:intergenic
AAATGCAACGAGGAAGCAGA+CGG 0.655539 4_1:+79191559 None:intergenic
TGATGATGTAGAATCTGCAG+CGG 0.679814 4_1:-79191240 Msa0575330:CDS
CAAAGTCAACAACTCTCCAG+AGG 0.691397 4_1:+79190940 None:intergenic
ATACGACAACTAGAATCACA+AGG 0.695372 4_1:-79191295 Msa0575330:CDS
AAGCCAATGACAGTTCGATG+AGG 0.700602 4_1:+79192578 None:intergenic
TACGACAACTAGAATCACAA+GGG 0.715521 4_1:-79191294 Msa0575330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTGTTTTTATTTTTTA+TGG + chr4_1:79190993-79191012 None:intergenic 10.0%
!! TAAAAAATAAAAACAAATTG+TGG - chr4_1:79190993-79191012 Msa0575330:three_prime_UTR 10.0%
!!! AAAAAAAAAGTATTGTAATA+TGG + chr4_1:79191534-79191553 None:intergenic 10.0%
!!! CAATTTGTTTTTATTTTTTA+TGG + chr4_1:79190993-79191012 None:intergenic 10.0%
!! TAAAAAATAAAAACAAATTG+TGG - chr4_1:79190993-79191012 Msa0575330:three_prime_UTR 10.0%
!!! AAAAAAAAAGTATTGTAATA+TGG + chr4_1:79191534-79191553 None:intergenic 10.0%
!!! TACTTTTTTTTTTCTTTTTG+AGG - chr4_1:79191542-79191561 Msa0575330:CDS 15.0%
!!! ACTTTTTTTTTTCTTTTTGA+GGG - chr4_1:79191543-79191562 Msa0575330:CDS 15.0%
!! AAATATAGCAATTGAATTAA+TGG + chr4_1:79192143-79192162 None:intergenic 15.0%
!!! TAAATTTTAGTTTCTTGTTA+AGG - chr4_1:79192887-79192906 Msa0575330:five_prime_UTR 15.0%
!!! AAATTTTAGTTTCTTGTTAA+GGG - chr4_1:79192888-79192907 Msa0575330:five_prime_UTR 15.0%
!!! TACTTTTTTTTTTCTTTTTG+AGG - chr4_1:79191542-79191561 Msa0575330:CDS 15.0%
!!! ACTTTTTTTTTTCTTTTTGA+GGG - chr4_1:79191543-79191562 Msa0575330:CDS 15.0%
!! AAATATAGCAATTGAATTAA+TGG + chr4_1:79192143-79192162 None:intergenic 15.0%
!!! TAAATTTTAGTTTCTTGTTA+AGG - chr4_1:79192887-79192906 Msa0575330:five_prime_UTR 15.0%
!!! AAATTTTAGTTTCTTGTTAA+GGG - chr4_1:79192888-79192907 Msa0575330:five_prime_UTR 15.0%
!! AATAATTACTACTTTGGATT+TGG + chr4_1:79191209-79191228 None:intergenic 20.0%
!! AATCCAAAGTAGTAATTATT+TGG - chr4_1:79191209-79191228 Msa0575330:CDS 20.0%
!! ACTAAACAATGAAAATAGAA+AGG + chr4_1:79191426-79191445 None:intergenic 20.0%
!! ACCATATTACAATACTTAAA+AGG - chr4_1:79191567-79191586 Msa0575330:CDS 20.0%
!! CCATATTACAATACTTAAAA+GGG - chr4_1:79191568-79191587 Msa0575330:CDS 20.0%
!! CACAAAATTAAATCCTAAAA+AGG + chr4_1:79192091-79192110 None:intergenic 20.0%
!!! AATTCAATTGCTATATTTTC+AGG - chr4_1:79192145-79192164 Msa0575330:intron 20.0%
!! AATAATTACTACTTTGGATT+TGG + chr4_1:79191209-79191228 None:intergenic 20.0%
!! AATCCAAAGTAGTAATTATT+TGG - chr4_1:79191209-79191228 Msa0575330:CDS 20.0%
!! ACTAAACAATGAAAATAGAA+AGG + chr4_1:79191426-79191445 None:intergenic 20.0%
!! ACCATATTACAATACTTAAA+AGG - chr4_1:79191567-79191586 Msa0575330:CDS 20.0%
!! CCATATTACAATACTTAAAA+GGG - chr4_1:79191568-79191587 Msa0575330:CDS 20.0%
!! CACAAAATTAAATCCTAAAA+AGG + chr4_1:79192091-79192110 None:intergenic 20.0%
!!! AATTCAATTGCTATATTTTC+AGG - chr4_1:79192145-79192164 Msa0575330:intron 20.0%
! CAAAGTAGTAATTATTTGGT+GGG - chr4_1:79191213-79191232 Msa0575330:CDS 25.0%
!!! CCCTTTTAAGTATTGTAATA+TGG + chr4_1:79191571-79191590 None:intergenic 25.0%
! AAACTTGAGCAATAAAAACT+CGG + chr4_1:79191669-79191688 None:intergenic 25.0%
! AAACTTGTGTAGAAAAATCT+TGG - chr4_1:79191904-79191923 Msa0575330:intron 25.0%
! CAAACAATAACTATGTTCTA+AGG + chr4_1:79192050-79192069 None:intergenic 25.0%
!! ATCATTCAGTTTGTACTTTT+TGG - chr4_1:79192595-79192614 Msa0575330:CDS 25.0%
! CAAAGTAGTAATTATTTGGT+GGG - chr4_1:79191213-79191232 Msa0575330:CDS 25.0%
!!! CCCTTTTAAGTATTGTAATA+TGG + chr4_1:79191571-79191590 None:intergenic 25.0%
! AAACTTGAGCAATAAAAACT+CGG + chr4_1:79191669-79191688 None:intergenic 25.0%
! AAACTTGTGTAGAAAAATCT+TGG - chr4_1:79191904-79191923 Msa0575330:intron 25.0%
! CAAACAATAACTATGTTCTA+AGG + chr4_1:79192050-79192069 None:intergenic 25.0%
!! ATCATTCAGTTTGTACTTTT+TGG - chr4_1:79192595-79192614 Msa0575330:CDS 25.0%
TCCATTTCAATTGGAAAAAC+TGG - chr4_1:79191023-79191042 Msa0575330:three_prime_UTR 30.0%
! ACCAGTTTTTCCAATTGAAA+TGG + chr4_1:79191027-79191046 None:intergenic 30.0%
!!! TCTCATGTTTGGTTTTAATG+AGG - chr4_1:79191062-79191081 Msa0575330:three_prime_UTR 30.0%
!! TTTAATGAGGTTGATGTCTT+TGG - chr4_1:79191075-79191094 Msa0575330:three_prime_UTR 30.0%
!! TTAATGAGGTTGATGTCTTT+GGG - chr4_1:79191076-79191095 Msa0575330:three_prime_UTR 30.0%
! CTTGATAGTACAAGATTCTA+TGG + chr4_1:79191165-79191184 None:intergenic 30.0%
CCACCAAATAATTACTACTT+TGG + chr4_1:79191215-79191234 None:intergenic 30.0%
CCAAAGTAGTAATTATTTGG+TGG - chr4_1:79191212-79191231 Msa0575330:CDS 30.0%
! TGTTCTTTTTGTATCATGCT+TGG - chr4_1:79191288-79191307 Msa0575330:CDS 30.0%
ACTTTGTTCTTGAGATTAAC+TGG - chr4_1:79191596-79191615 Msa0575330:CDS 30.0%
AGATTAACTGGAATTTATCC+TGG - chr4_1:79191608-79191627 Msa0575330:CDS 30.0%
! TACCTAAAGTTTAAGACAGT+AGG - chr4_1:79191771-79191790 Msa0575330:intron 30.0%
TTCCTACTGTCTTAAACTTT+AGG + chr4_1:79191776-79191795 None:intergenic 30.0%
AATTGTGTTCTGCAAATGAT+AGG - chr4_1:79191794-79191813 Msa0575330:intron 30.0%
!! ACCAATTTACTGATTTACCA+CGG + chr4_1:79191875-79191894 None:intergenic 30.0%
ATCACACTAAGTTCACTTAT+TGG - chr4_1:79192248-79192267 Msa0575330:intron 30.0%
!! TGAAGAAGTACTTTGAAGTA+TGG + chr4_1:79192315-79192334 None:intergenic 30.0%
! CAGAGATAAAAAGGCATATA+TGG - chr4_1:79192442-79192461 Msa0575330:intron 30.0%
! ATTCGTAGCAATTACAGTTT+TGG - chr4_1:79192770-79192789 Msa0575330:five_prime_UTR 30.0%
TTCAATAAGAGTAGAGTTCT+AGG - chr4_1:79192861-79192880 Msa0575330:five_prime_UTR 30.0%
TCCATTTCAATTGGAAAAAC+TGG - chr4_1:79191023-79191042 Msa0575330:three_prime_UTR 30.0%
! ACCAGTTTTTCCAATTGAAA+TGG + chr4_1:79191027-79191046 None:intergenic 30.0%
!!! TCTCATGTTTGGTTTTAATG+AGG - chr4_1:79191062-79191081 Msa0575330:three_prime_UTR 30.0%
!! TTTAATGAGGTTGATGTCTT+TGG - chr4_1:79191075-79191094 Msa0575330:three_prime_UTR 30.0%
!! TTAATGAGGTTGATGTCTTT+GGG - chr4_1:79191076-79191095 Msa0575330:three_prime_UTR 30.0%
! CTTGATAGTACAAGATTCTA+TGG + chr4_1:79191165-79191184 None:intergenic 30.0%
CCACCAAATAATTACTACTT+TGG + chr4_1:79191215-79191234 None:intergenic 30.0%
CCAAAGTAGTAATTATTTGG+TGG - chr4_1:79191212-79191231 Msa0575330:CDS 30.0%
! TGTTCTTTTTGTATCATGCT+TGG - chr4_1:79191288-79191307 Msa0575330:CDS 30.0%
ACTTTGTTCTTGAGATTAAC+TGG - chr4_1:79191596-79191615 Msa0575330:CDS 30.0%
AGATTAACTGGAATTTATCC+TGG - chr4_1:79191608-79191627 Msa0575330:CDS 30.0%
! TACCTAAAGTTTAAGACAGT+AGG - chr4_1:79191771-79191790 Msa0575330:intron 30.0%
TTCCTACTGTCTTAAACTTT+AGG + chr4_1:79191776-79191795 None:intergenic 30.0%
AATTGTGTTCTGCAAATGAT+AGG - chr4_1:79191794-79191813 Msa0575330:intron 30.0%
!! ACCAATTTACTGATTTACCA+CGG + chr4_1:79191875-79191894 None:intergenic 30.0%
ATCACACTAAGTTCACTTAT+TGG - chr4_1:79192248-79192267 Msa0575330:intron 30.0%
!! TGAAGAAGTACTTTGAAGTA+TGG + chr4_1:79192315-79192334 None:intergenic 30.0%
! CAGAGATAAAAAGGCATATA+TGG - chr4_1:79192442-79192461 Msa0575330:intron 30.0%
! ATTCGTAGCAATTACAGTTT+TGG - chr4_1:79192770-79192789 Msa0575330:five_prime_UTR 30.0%
TTCAATAAGAGTAGAGTTCT+AGG - chr4_1:79192861-79192880 Msa0575330:five_prime_UTR 30.0%
GGTGAATCTTCCATTTCAAT+TGG - chr4_1:79191014-79191033 Msa0575330:three_prime_UTR 35.0%
!! TTTGGGTTGATCTCATTACT+AGG - chr4_1:79191093-79191112 Msa0575330:three_prime_UTR 35.0%
! GTAGTAATTATTTGGTGGGA+TGG - chr4_1:79191217-79191236 Msa0575330:CDS 35.0%
GAGTATGTGTTTGTACCATT+GGG - chr4_1:79191257-79191276 Msa0575330:CDS 35.0%
GTTTGTACCATTGGGATTAT+TGG - chr4_1:79191265-79191284 Msa0575330:CDS 35.0%
AAGAACACCAATAATCCCAA+TGG + chr4_1:79191275-79191294 None:intergenic 35.0%
TAACTGGAATTTATCCTGGT+TGG - chr4_1:79191612-79191631 Msa0575330:CDS 35.0%
TCCGTGGTAAATCAGTAAAT+TGG - chr4_1:79191871-79191890 Msa0575330:intron 35.0%
! TATTACTGCATTACTGACTG+AGG - chr4_1:79191931-79191950 Msa0575330:intron 35.0%
GTTTGTTCTACCAACGTATT+TGG - chr4_1:79192065-79192084 Msa0575330:intron 35.0%
TGATATTGTTGCGGATAGTT+TGG + chr4_1:79192199-79192218 None:intergenic 35.0%
AACTATCCGCAACAATATCA+TGG - chr4_1:79192199-79192218 Msa0575330:intron 35.0%
CATTGCATAAAAATGCAACG+AGG + chr4_1:79192367-79192386 None:intergenic 35.0%
ACCTACACTCAGAGATAAAA+AGG - chr4_1:79192433-79192452 Msa0575330:intron 35.0%
TATCATTTCCTCTCTTCCTA+TGG - chr4_1:79192531-79192550 Msa0575330:intron 35.0%
CTCTCTTCCTATGGATTTAT+GGG - chr4_1:79192540-79192559 Msa0575330:CDS 35.0%
CAACAAGCAAACATTGGTAT+AGG + chr4_1:79192566-79192585 None:intergenic 35.0%
ATACGACAACTAGAATCACA+AGG - chr4_1:79192618-79192637 Msa0575330:CDS 35.0%
TACGACAACTAGAATCACAA+GGG - chr4_1:79192619-79192638 Msa0575330:CDS 35.0%
TCATAGTGACTCATTCAGAA+AGG - chr4_1:79192646-79192665 Msa0575330:CDS 35.0%
GCTGATTATTATCCTTTAGC+TGG - chr4_1:79192698-79192717 Msa0575330:exon 35.0%
GCTAAATTGTTACCAGCTAA+AGG + chr4_1:79192713-79192732 None:intergenic 35.0%
AAGTATATGAACATGTGCCT+TGG - chr4_1:79192821-79192840 Msa0575330:five_prime_UTR 35.0%
AGGGTTGTCGAATTCATTTA+AGG - chr4_1:79192907-79192926 Msa0575330:five_prime_UTR 35.0%
GGTGAATCTTCCATTTCAAT+TGG - chr4_1:79191014-79191033 Msa0575330:three_prime_UTR 35.0%
!! TTTGGGTTGATCTCATTACT+AGG - chr4_1:79191093-79191112 Msa0575330:three_prime_UTR 35.0%
! GTAGTAATTATTTGGTGGGA+TGG - chr4_1:79191217-79191236 Msa0575330:CDS 35.0%
GAGTATGTGTTTGTACCATT+GGG - chr4_1:79191257-79191276 Msa0575330:CDS 35.0%
GTTTGTACCATTGGGATTAT+TGG - chr4_1:79191265-79191284 Msa0575330:CDS 35.0%
AAGAACACCAATAATCCCAA+TGG + chr4_1:79191275-79191294 None:intergenic 35.0%
TAACTGGAATTTATCCTGGT+TGG - chr4_1:79191612-79191631 Msa0575330:CDS 35.0%
TCCGTGGTAAATCAGTAAAT+TGG - chr4_1:79191871-79191890 Msa0575330:intron 35.0%
! TATTACTGCATTACTGACTG+AGG - chr4_1:79191931-79191950 Msa0575330:intron 35.0%
GTTTGTTCTACCAACGTATT+TGG - chr4_1:79192065-79192084 Msa0575330:intron 35.0%
TGATATTGTTGCGGATAGTT+TGG + chr4_1:79192199-79192218 None:intergenic 35.0%
AACTATCCGCAACAATATCA+TGG - chr4_1:79192199-79192218 Msa0575330:intron 35.0%
CATTGCATAAAAATGCAACG+AGG + chr4_1:79192367-79192386 None:intergenic 35.0%
ACCTACACTCAGAGATAAAA+AGG - chr4_1:79192433-79192452 Msa0575330:intron 35.0%
TATCATTTCCTCTCTTCCTA+TGG - chr4_1:79192531-79192550 Msa0575330:intron 35.0%
CTCTCTTCCTATGGATTTAT+GGG - chr4_1:79192540-79192559 Msa0575330:CDS 35.0%
CAACAAGCAAACATTGGTAT+AGG + chr4_1:79192566-79192585 None:intergenic 35.0%
ATACGACAACTAGAATCACA+AGG - chr4_1:79192618-79192637 Msa0575330:CDS 35.0%
TACGACAACTAGAATCACAA+GGG - chr4_1:79192619-79192638 Msa0575330:CDS 35.0%
TCATAGTGACTCATTCAGAA+AGG - chr4_1:79192646-79192665 Msa0575330:CDS 35.0%
GCTGATTATTATCCTTTAGC+TGG - chr4_1:79192698-79192717 Msa0575330:exon 35.0%
GCTAAATTGTTACCAGCTAA+AGG + chr4_1:79192713-79192732 None:intergenic 35.0%
AAGTATATGAACATGTGCCT+TGG - chr4_1:79192821-79192840 Msa0575330:five_prime_UTR 35.0%
AGGGTTGTCGAATTCATTTA+AGG - chr4_1:79192907-79192926 Msa0575330:five_prime_UTR 35.0%
!! GCAAGTTGCAACTTGTTGAT+AGG + chr4_1:79190935-79190954 None:intergenic 40.0%
GGAGTATGTGTTTGTACCAT+TGG - chr4_1:79191256-79191275 Msa0575330:CDS 40.0%
!! TCGGTGTTTTAGATTTGCGT+TGG + chr4_1:79191650-79191669 None:intergenic 40.0%
TGTGTTCTGCAAATGATAGG+TGG - chr4_1:79191797-79191816 Msa0575330:intron 40.0%
TCTTCTGCTGTCTATCTCAA+AGG - chr4_1:79191987-79192006 Msa0575330:intron 40.0%
CCTAAAAAGGCCAAATACGT+TGG + chr4_1:79192078-79192097 None:intergenic 40.0%
!! CCAACGTATTTGGCCTTTTT+AGG - chr4_1:79192075-79192094 Msa0575330:intron 40.0%
TTTCAGGATCCCTTCAAGAA+CGG - chr4_1:79192161-79192180 Msa0575330:intron 40.0%
TAGACGCCATGATATTGTTG+CGG + chr4_1:79192208-79192227 None:intergenic 40.0%
! GCCTTTTTATCTCTGAGTGT+AGG + chr4_1:79192437-79192456 None:intergenic 40.0%
GTAAAATGCTCGCAATCCAA+GGG + chr4_1:79192510-79192529 None:intergenic 40.0%
AGTAAAATGCTCGCAATCCA+AGG + chr4_1:79192511-79192530 None:intergenic 40.0%
CCATAAATCCATAGGAAGAG+AGG + chr4_1:79192542-79192561 None:intergenic 40.0%
CCTCTCTTCCTATGGATTTA+TGG - chr4_1:79192539-79192558 Msa0575330:CDS 40.0%
GTATAGGCCCATAAATCCAT+AGG + chr4_1:79192550-79192569 None:intergenic 40.0%
AAGCAGCAACAAGCAAACAT+TGG + chr4_1:79192572-79192591 None:intergenic 40.0%
! TGATGATGTAGAATCTGCAG+CGG - chr4_1:79192673-79192692 Msa0575330:CDS 40.0%
TATGAACATGTGCCTTGGAT+TGG - chr4_1:79192826-79192845 Msa0575330:five_prime_UTR 40.0%
TCATTCCAACATCCAATCCA+AGG + chr4_1:79192841-79192860 None:intergenic 40.0%
GAGGTACAACACACATGAAT+GGG + chr4_1:79192957-79192976 None:intergenic 40.0%
! AGAGGTACAACACACATGAA+TGG + chr4_1:79192958-79192977 None:intergenic 40.0%
ATTCATGTGTGTTGTACCTC+TGG - chr4_1:79192957-79192976 Msa0575330:five_prime_UTR 40.0%
!! GCAAGTTGCAACTTGTTGAT+AGG + chr4_1:79190935-79190954 None:intergenic 40.0%
GGAGTATGTGTTTGTACCAT+TGG - chr4_1:79191256-79191275 Msa0575330:CDS 40.0%
!! TCGGTGTTTTAGATTTGCGT+TGG + chr4_1:79191650-79191669 None:intergenic 40.0%
TGTGTTCTGCAAATGATAGG+TGG - chr4_1:79191797-79191816 Msa0575330:intron 40.0%
TCTTCTGCTGTCTATCTCAA+AGG - chr4_1:79191987-79192006 Msa0575330:intron 40.0%
CCTAAAAAGGCCAAATACGT+TGG + chr4_1:79192078-79192097 None:intergenic 40.0%
!! CCAACGTATTTGGCCTTTTT+AGG - chr4_1:79192075-79192094 Msa0575330:intron 40.0%
TTTCAGGATCCCTTCAAGAA+CGG - chr4_1:79192161-79192180 Msa0575330:intron 40.0%
TAGACGCCATGATATTGTTG+CGG + chr4_1:79192208-79192227 None:intergenic 40.0%
! GCCTTTTTATCTCTGAGTGT+AGG + chr4_1:79192437-79192456 None:intergenic 40.0%
GTAAAATGCTCGCAATCCAA+GGG + chr4_1:79192510-79192529 None:intergenic 40.0%
AGTAAAATGCTCGCAATCCA+AGG + chr4_1:79192511-79192530 None:intergenic 40.0%
CCATAAATCCATAGGAAGAG+AGG + chr4_1:79192542-79192561 None:intergenic 40.0%
CCTCTCTTCCTATGGATTTA+TGG - chr4_1:79192539-79192558 Msa0575330:CDS 40.0%
GTATAGGCCCATAAATCCAT+AGG + chr4_1:79192550-79192569 None:intergenic 40.0%
AAGCAGCAACAAGCAAACAT+TGG + chr4_1:79192572-79192591 None:intergenic 40.0%
! TGATGATGTAGAATCTGCAG+CGG - chr4_1:79192673-79192692 Msa0575330:CDS 40.0%
TATGAACATGTGCCTTGGAT+TGG - chr4_1:79192826-79192845 Msa0575330:five_prime_UTR 40.0%
TCATTCCAACATCCAATCCA+AGG + chr4_1:79192841-79192860 None:intergenic 40.0%
GAGGTACAACACACATGAAT+GGG + chr4_1:79192957-79192976 None:intergenic 40.0%
! AGAGGTACAACACACATGAA+TGG + chr4_1:79192958-79192977 None:intergenic 40.0%
ATTCATGTGTGTTGTACCTC+TGG - chr4_1:79192957-79192976 Msa0575330:five_prime_UTR 40.0%
! GTCAGTGTCACTCTCATGTT+TGG - chr4_1:79191051-79191070 Msa0575330:three_prime_UTR 45.0%
GAACCTCATCGAACTGTCAT+TGG - chr4_1:79191332-79191351 Msa0575330:CDS 45.0%
AAGCCAATGACAGTTCGATG+AGG + chr4_1:79191338-79191357 None:intergenic 45.0%
ACGCTGAGTCACGTATTCAA+TGG - chr4_1:79191360-79191379 Msa0575330:CDS 45.0%
CGCTGAGTCACGTATTCAAT+GGG - chr4_1:79191361-79191380 Msa0575330:CDS 45.0%
TGAGTGACTCACACTCATCA+TGG + chr4_1:79191393-79191412 None:intergenic 45.0%
ATTGGCAGTTTGGCACTTCT+TGG + chr4_1:79191826-79191845 None:intergenic 45.0%
TGCCAAACTGCCAATAGAGT+TGG - chr4_1:79191831-79191850 Msa0575330:intron 45.0%
GCCAAACTGCCAATAGAGTT+GGG - chr4_1:79191832-79191851 Msa0575330:intron 45.0%
TCCCAACTCTATTGGCAGTT+TGG + chr4_1:79191836-79191855 None:intergenic 45.0%
TGCCAATAGAGTTGGGACTT+GGG - chr4_1:79191839-79191858 Msa0575330:intron 45.0%
! TCCCTTCAAGAACGGTGTTT+TGG - chr4_1:79192169-79192188 Msa0575330:intron 45.0%
GCCAAAACACCGTTCTTGAA+GGG + chr4_1:79192173-79192192 None:intergenic 45.0%
!! CTTCAAGAACGGTGTTTTGG+CGG - chr4_1:79192172-79192191 Msa0575330:intron 45.0%
! GAATTTTCGCTAGCAGTTCG+TGG - chr4_1:79192270-79192289 Msa0575330:intron 45.0%
AAATGCAACGAGGAAGCAGA+CGG + chr4_1:79192357-79192376 None:intergenic 45.0%
! ATGTGCCTTGGATTGGATGT+TGG - chr4_1:79192833-79192852 Msa0575330:five_prime_UTR 45.0%
CAAAGTCAACAACTCTCCAG+AGG + chr4_1:79192976-79192995 None:intergenic 45.0%
! GTCAGTGTCACTCTCATGTT+TGG - chr4_1:79191051-79191070 Msa0575330:three_prime_UTR 45.0%
GAACCTCATCGAACTGTCAT+TGG - chr4_1:79191332-79191351 Msa0575330:CDS 45.0%
AAGCCAATGACAGTTCGATG+AGG + chr4_1:79191338-79191357 None:intergenic 45.0%
ACGCTGAGTCACGTATTCAA+TGG - chr4_1:79191360-79191379 Msa0575330:CDS 45.0%
CGCTGAGTCACGTATTCAAT+GGG - chr4_1:79191361-79191380 Msa0575330:CDS 45.0%
TGAGTGACTCACACTCATCA+TGG + chr4_1:79191393-79191412 None:intergenic 45.0%
ATTGGCAGTTTGGCACTTCT+TGG + chr4_1:79191826-79191845 None:intergenic 45.0%
TGCCAAACTGCCAATAGAGT+TGG - chr4_1:79191831-79191850 Msa0575330:intron 45.0%
GCCAAACTGCCAATAGAGTT+GGG - chr4_1:79191832-79191851 Msa0575330:intron 45.0%
TCCCAACTCTATTGGCAGTT+TGG + chr4_1:79191836-79191855 None:intergenic 45.0%
TGCCAATAGAGTTGGGACTT+GGG - chr4_1:79191839-79191858 Msa0575330:intron 45.0%
! TCCCTTCAAGAACGGTGTTT+TGG - chr4_1:79192169-79192188 Msa0575330:intron 45.0%
GCCAAAACACCGTTCTTGAA+GGG + chr4_1:79192173-79192192 None:intergenic 45.0%
!! CTTCAAGAACGGTGTTTTGG+CGG - chr4_1:79192172-79192191 Msa0575330:intron 45.0%
! GAATTTTCGCTAGCAGTTCG+TGG - chr4_1:79192270-79192289 Msa0575330:intron 45.0%
AAATGCAACGAGGAAGCAGA+CGG + chr4_1:79192357-79192376 None:intergenic 45.0%
! ATGTGCCTTGGATTGGATGT+TGG - chr4_1:79192833-79192852 Msa0575330:five_prime_UTR 45.0%
CAAAGTCAACAACTCTCCAG+AGG + chr4_1:79192976-79192995 None:intergenic 45.0%
GATGGAGAACAACGAGCATC+TGG - chr4_1:79191235-79191254 Msa0575330:CDS 50.0%
GGCTATGTTCTCAACCAACC+AGG + chr4_1:79191629-79191648 None:intergenic 50.0%
CTGCCAATAGAGTTGGGACT+TGG - chr4_1:79191838-79191857 Msa0575330:intron 50.0%
CTCCCAAGTCCCAACTCTAT+TGG + chr4_1:79191844-79191863 None:intergenic 50.0%
CAATAGAGTTGGGACTTGGG+AGG - chr4_1:79191842-79191861 Msa0575330:intron 50.0%
CGCCAAAACACCGTTCTTGA+AGG + chr4_1:79192174-79192193 None:intergenic 50.0%
TGAATCGCGCTAGTCACTCT+TGG - chr4_1:79192483-79192502 Msa0575330:intron 50.0%
GATGGAGAACAACGAGCATC+TGG - chr4_1:79191235-79191254 Msa0575330:CDS 50.0%
GGCTATGTTCTCAACCAACC+AGG + chr4_1:79191629-79191648 None:intergenic 50.0%
CTGCCAATAGAGTTGGGACT+TGG - chr4_1:79191838-79191857 Msa0575330:intron 50.0%
CTCCCAAGTCCCAACTCTAT+TGG + chr4_1:79191844-79191863 None:intergenic 50.0%
CAATAGAGTTGGGACTTGGG+AGG - chr4_1:79191842-79191861 Msa0575330:intron 50.0%
CGCCAAAACACCGTTCTTGA+AGG + chr4_1:79192174-79192193 None:intergenic 50.0%
TGAATCGCGCTAGTCACTCT+TGG - chr4_1:79192483-79192502 Msa0575330:intron 50.0%
GGCAGTTTGGCACTTCTTGG+CGG + chr4_1:79191823-79191842 None:intergenic 55.0%
AGTTGGGACTTGGGAGGTCT+TGG - chr4_1:79191848-79191867 Msa0575330:intron 55.0%
GCTAGTCACTCTTGGTCCCT+TGG - chr4_1:79192491-79192510 Msa0575330:intron 55.0%
GGCAGTTTGGCACTTCTTGG+CGG + chr4_1:79191823-79191842 None:intergenic 55.0%
AGTTGGGACTTGGGAGGTCT+TGG - chr4_1:79191848-79191867 Msa0575330:intron 55.0%
GCTAGTCACTCTTGGTCCCT+TGG - chr4_1:79192491-79192510 Msa0575330:intron 55.0%
ACTTGGGAGGTCTTGGTCCG+TGG - chr4_1:79191855-79191874 Msa0575330:intron 60.0%
ACTTGGGAGGTCTTGGTCCG+TGG - chr4_1:79191855-79191874 Msa0575330:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 79190926 79193009 79190926 ID=Msa0575330;Name=Msa0575330
chr4_1 mRNA 79190926 79193009 79190926 ID=Msa0575330-mRNA-1;Parent=Msa0575330;Name=Msa0575330-mRNA-1;_AED=0.37;_eAED=0.37;_QI=310|1|1|1|1|1|2|249|252
chr4_1 exon 79190926 79191768 79190926 ID=Msa0575330-mRNA-1:exon:28235;Parent=Msa0575330-mRNA-1
chr4_1 exon 79192535 79193009 79192535 ID=Msa0575330-mRNA-1:exon:28234;Parent=Msa0575330-mRNA-1
chr4_1 five_prime_UTR 79192700 79193009 79192700 ID=Msa0575330-mRNA-1:five_prime_utr;Parent=Msa0575330-mRNA-1
chr4_1 CDS 79192535 79192699 79192535 ID=Msa0575330-mRNA-1:cds;Parent=Msa0575330-mRNA-1
chr4_1 CDS 79191175 79191768 79191175 ID=Msa0575330-mRNA-1:cds;Parent=Msa0575330-mRNA-1
chr4_1 three_prime_UTR 79190926 79191174 79190926 ID=Msa0575330-mRNA-1:three_prime_utr;Parent=Msa0575330-mRNA-1
Gene Sequence

>Msa0575330

ATGGAGAACAACGAGCATCTGGAGTATGTGTTTGTACCATTGGGATTATTGGTGTTCTTTTTGTATCATGCTTGGCTTCTCTTTACTATTCTTCGTGAACCTCATCGAACTGTCATTGGCTTAAACGCTGAGTCACGTATTCAATGGGTTCATGCCATGATGAGTGATCCCTTCAAGAACGGTGTTTTGGCGGTCCAAACTATCCGCAACAATATCATGGCGTCTAATCTTCTTGCTACAACAGCAATCACACTAAGTTCACTTATTGGAATTTTCGCTAGCAGTTCGTGGAGTTCTGATGATACATCTTCCATACTTCAAAGTACTTCTTCAATCAAACGTATTTCGATTACCGTCTGCTTCCTCGTTGCATTTTTATGCAATGTTCAGTCTATAAGATGCTATTGTCATGTCAGCTTTTTAATCACTGCACCTACACTCAGAGATAAAAAGGCATATATGGAATATATTGCTAAGACATTGAATCGCGCTAGTCACTCTTGGTCCCTTGGATTGCGAGCATTTTACTTATCATTTCCTCTCTTCCTATGGATTTATGGGCCTATACCAATGTTTGCTTGTTGCTGCTTGACATCATTCAGTTTGTACTTTTTGGATACGACAACTAGAATCACAAGGGATCTTCATAGTGACTCATTCAGAAAGGAGAGTGATGATGTAGAATCTGCAGCGGACGCTGATTATTATCCTTTAGCTGGTAACAATTTAGCTCAAAATGCAGCTGTAGATAATGTATAA
Protein sequence

>Msa0575330

MENNEHLEYVFVPLGLLVFFLYHAWLLFTILREPHRTVIGLNAESRIQWVHAMMSDPFKNGVLAVQTIRNNIMASNLLATTAITLSSLIGIFASSSWSSDDTSSILQSTSSIKRISITVCFLVAFLCNVQSIRCYCHVSFLITAPTLRDKKAYMEYIAKTLNRASHSWSLGLRAFYLSFPLFLWIYGPIPMFACCCLTSFSLYFLDTTTRITRDLHSDSFRKESDDVESAADADYYPLAGNNLAQNAAVDNV*