AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0576520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576520 MtrunA17_Chr8g0390351 78.169 142 9 1 28 169 476 595 3.39e-64 206
Msa0576520 MtrunA17_Chr6g0485811 48.837 86 33 2 28 113 522 596 2.13e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 64 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGTCTTGTTTCAGAAAA+TGG 0.136929 4_1:-80591428 Msa0576520:CDS
ACAAAATTGATAAAAGAAAA+TGG 0.262257 4_1:+80591218 None:intergenic
TTGAGTCTCTAACTCACTTT+TGG 0.267883 4_1:+80591781 None:intergenic
TGCTTTGCCGACGTTATTAC+AGG 0.297076 4_1:+80591688 None:intergenic
AATTTAGAGAACTGTGATAC+TGG 0.327348 4_1:-80592179 Msa0576520:CDS
ATTCAGTTTGCATCGAAGAT+AGG 0.328810 4_1:-80592137 Msa0576520:CDS
TATATCTATTGTTCGACCTT+CGG 0.358406 4_1:+80591395 None:intergenic
GCAGTCTTGTTTCAGAAAAT+GGG 0.359660 4_1:-80591427 Msa0576520:CDS
CACCATCACTTCAACGCCTT+TGG 0.361349 4_1:-80591492 Msa0576520:CDS
CACCATCACTTCAACACCTT+TGG 0.384125 4_1:-80591525 Msa0576520:CDS
CACCATCACTTCAACACCTT+TGG 0.384125 4_1:-80591558 Msa0576520:CDS
CACCATCACTTCAACACCTT+TGG 0.384125 4_1:-80591591 Msa0576520:CDS
TTTCAGTGCGATTGATGATT+GGG 0.398288 4_1:+80591753 None:intergenic
CACCATCACTTCAGCACCTA+TGG 0.418273 4_1:-80591657 Msa0576520:CDS
AAAAGAAAATGGATCTTAGT+TGG 0.421180 4_1:+80591229 None:intergenic
CGTCACTTCAACAACACCTT+TGG 0.427483 4_1:-80591456 Msa0576520:CDS
TGTATGTGTGCTCTCTGTAA+TGG 0.439729 4_1:-80591314 Msa0576520:three_prime_UTR
GGCAGCGCAACAGCAAGGAC+TGG 0.462015 4_1:-80591717 Msa0576520:CDS
AAAATGGATCTTAGTTGGAC+TGG 0.462910 4_1:+80591234 None:intergenic
CTGTTGCGCTGCCTCAGTAC+TGG 0.464401 4_1:+80591727 None:intergenic
GGTCGAACAATAGATATAGA+AGG 0.470732 4_1:-80591390 Msa0576520:exon
TTACTTCCAAACGGTACCTC+TGG 0.474146 4_1:+80591351 None:intergenic
TTCAGTTTGCATCGAAGATA+GGG 0.475902 4_1:-80592136 Msa0576520:CDS
GCAGCGCAACAGCAAGGACT+GGG 0.486221 4_1:-80591716 Msa0576520:CDS
AGATGACCAGAGGTACCGTT+TGG 0.492086 4_1:-80591357 Msa0576520:three_prime_UTR
CACCATCACTTCAACACCTA+TGG 0.496104 4_1:-80591624 Msa0576520:CDS
TTTCTGTAGGCTTTAACTGT+AGG 0.498182 4_1:+80591259 None:intergenic
GACCAAAGGCGTTGAAGTGA+TGG 0.522108 4_1:+80591490 None:intergenic
CAGTCTTGTTTCAGAAAATG+GGG 0.524569 4_1:-80591426 Msa0576520:CDS
AAGTGAGTTAGAGACTCAAA+AGG 0.552669 4_1:-80591777 Msa0576520:CDS
TTGAAGTGATGGTGTTGCAT+GGG 0.553400 4_1:+80591534 None:intergenic
TTGAAGTGATGGTGTTGCAT+GGG 0.553400 4_1:+80591567 None:intergenic
GTTGAAGTGATGGTGTTGCA+TGG 0.560665 4_1:+80591500 None:intergenic
GTTGAAGTGATGGTGTTGCA+TGG 0.579282 4_1:+80591533 None:intergenic
GTTGAAGTGATGGTGTTGCA+TGG 0.579282 4_1:+80591566 None:intergenic
ACGGTGTTGCAAGGGACCAA+AGG 0.581749 4_1:+80591476 None:intergenic
AATAACGTCGGCAAAGCAGT+TGG 0.582782 4_1:-80591683 Msa0576520:CDS
GTTGAAGTGACGGTGTTGCA+AGG 0.590921 4_1:+80591467 None:intergenic
TTGAAGTGATGGTGTTGCAT+GGG 0.595326 4_1:+80591501 None:intergenic
ACAAGACTGCAAGGGACCAA+AGG 0.597556 4_1:+80591440 None:intergenic
GACCATAGGTGCTGAAGTGA+TGG 0.600765 4_1:+80591655 None:intergenic
GACCATAGGTGTTGAAGTGA+TGG 0.600889 4_1:+80591622 None:intergenic
ATGGTGTTGCAAGGGACCAT+AGG 0.603345 4_1:+80591608 None:intergenic
ATGGTGTTGCAAGGGACCAT+AGG 0.603345 4_1:+80591641 None:intergenic
TTTGCATCGAAGATAGGGAA+TGG 0.606691 4_1:-80592131 Msa0576520:intron
TTTGCCGACGTTATTACAGG+TGG 0.614550 4_1:+80591691 None:intergenic
TTGAAGTGACGGTGTTGCAA+GGG 0.618089 4_1:+80591468 None:intergenic
AAATGGGGAAATTAAGCCGA+AGG 0.620465 4_1:-80591411 Msa0576520:CDS
GACCAAAGGTGTTGAAGTGA+TGG 0.620830 4_1:+80591523 None:intergenic
GACCAAAGGTGTTGAAGTGA+TGG 0.620830 4_1:+80591556 None:intergenic
GACCAAAGGTGTTGAAGTGA+TGG 0.620830 4_1:+80591589 None:intergenic
GTTGAAGTGATGGTGTTGCA+AGG 0.625625 4_1:+80591599 None:intergenic
GTTGAAGTGATGGTGTTGCA+AGG 0.625625 4_1:+80591632 None:intergenic
TTGAAGTGATGGTGTTGCAA+GGG 0.633210 4_1:+80591600 None:intergenic
TTGAAGTGATGGTGTTGCAA+GGG 0.633210 4_1:+80591633 None:intergenic
ACTGAGGCAGCGCAACAGCA+AGG 0.636738 4_1:-80591722 Msa0576520:CDS
ATGGTGTTGCATGGGACCAA+AGG 0.637170 4_1:+80591509 None:intergenic
ATGGTGTTGCATGGGACCAA+AGG 0.637170 4_1:+80591542 None:intergenic
ATGGTGTTGCATGGGACCAA+AGG 0.637170 4_1:+80591575 None:intergenic
CAAAGGTGTTGTTGAAGTGA+CGG 0.642763 4_1:+80591457 None:intergenic
TTTCTGAAACAAGACTGCAA+GGG 0.692177 4_1:+80591432 None:intergenic
GGATCCACCTGTAATAACGT+CGG 0.698709 4_1:-80591695 Msa0576520:CDS
CACTGAAAATACCAGTACTG+AGG 0.701584 4_1:-80591738 Msa0576520:CDS
AGTCTAACTTAGATGACCAG+AGG 0.705976 4_1:-80591367 Msa0576520:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTGAATATTTTTTGAAAAT+TGG + chr4_1:80591213-80591232 None:intergenic 15.0%
!! ACAAAATTGATAAAAGAAAA+TGG + chr4_1:80592148-80592167 None:intergenic 15.0%
!!! ACTGAATATTTTTTGAAAAT+TGG + chr4_1:80591213-80591232 None:intergenic 15.0%
!! ACAAAATTGATAAAAGAAAA+TGG + chr4_1:80592148-80592167 None:intergenic 15.0%
!! TGAAATTAGGAAAAGAAATT+AGG + chr4_1:80591540-80591559 None:intergenic 20.0%
!! TGAAATTAGGAAAAGAAATT+AGG + chr4_1:80591540-80591559 None:intergenic 20.0%
! AATAAATACACTACAGAAGT+TGG + chr4_1:80591427-80591446 None:intergenic 25.0%
!! TAAAGTGTTACAAATAAAGC+AGG + chr4_1:80591512-80591531 None:intergenic 25.0%
! AAAAGAAAATGGATCTTAGT+TGG + chr4_1:80592137-80592156 None:intergenic 25.0%
! TAAATTGAAAGTTGAATGCA+TGG + chr4_1:80591170-80591189 None:intergenic 25.0%
! AATAAATACACTACAGAAGT+TGG + chr4_1:80591427-80591446 None:intergenic 25.0%
!! TAAAGTGTTACAAATAAAGC+AGG + chr4_1:80591512-80591531 None:intergenic 25.0%
! AAAAGAAAATGGATCTTAGT+TGG + chr4_1:80592137-80592156 None:intergenic 25.0%
AATTTAGAGAACTGTGATAC+TGG - chr4_1:80591184-80591203 Msa0576520:three_prime_UTR 30.0%
!! ATGGTTCTGAACTTTGATAA+AGG - chr4_1:80591251-80591270 Msa0576520:three_prime_UTR 30.0%
TATATCTATTGTTCGACCTT+CGG + chr4_1:80591971-80591990 None:intergenic 30.0%
!!! CTTCTAGTTTTACTTCCAAA+CGG + chr4_1:80592024-80592043 None:intergenic 30.0%
! CATTGTCATTCTTCATTTTC+TGG - chr4_1:80592077-80592096 Msa0576520:intron 30.0%
AATTTAGAGAACTGTGATAC+TGG - chr4_1:80591184-80591203 Msa0576520:three_prime_UTR 30.0%
!! ATGGTTCTGAACTTTGATAA+AGG - chr4_1:80591251-80591270 Msa0576520:three_prime_UTR 30.0%
TATATCTATTGTTCGACCTT+CGG + chr4_1:80591971-80591990 None:intergenic 30.0%
!!! CTTCTAGTTTTACTTCCAAA+CGG + chr4_1:80592024-80592043 None:intergenic 30.0%
! CATTGTCATTCTTCATTTTC+TGG - chr4_1:80592077-80592096 Msa0576520:intron 30.0%
ATTCAGTTTGCATCGAAGAT+AGG - chr4_1:80591226-80591245 Msa0576520:three_prime_UTR 35.0%
TTCAGTTTGCATCGAAGATA+GGG - chr4_1:80591227-80591246 Msa0576520:three_prime_UTR 35.0%
!! AACACTTCGTCTCTGAAATT+AGG + chr4_1:80591553-80591572 None:intergenic 35.0%
! TTGAGTCTCTAACTCACTTT+TGG + chr4_1:80591585-80591604 None:intergenic 35.0%
AAGTGAGTTAGAGACTCAAA+AGG - chr4_1:80591586-80591605 Msa0576520:CDS 35.0%
! TTTCAGTGCGATTGATGATT+GGG + chr4_1:80591613-80591632 None:intergenic 35.0%
!! TTTTCAGTGCGATTGATGAT+TGG + chr4_1:80591614-80591633 None:intergenic 35.0%
!! TTTCTGAAACAAGACTGCAA+GGG + chr4_1:80591934-80591953 None:intergenic 35.0%
!!! TTTTCTGAAACAAGACTGCA+AGG + chr4_1:80591935-80591954 None:intergenic 35.0%
!! TGCAGTCTTGTTTCAGAAAA+TGG - chr4_1:80591935-80591954 Msa0576520:intron 35.0%
!! GCAGTCTTGTTTCAGAAAAT+GGG - chr4_1:80591936-80591955 Msa0576520:intron 35.0%
!! CAGTCTTGTTTCAGAAAATG+GGG - chr4_1:80591937-80591956 Msa0576520:intron 35.0%
GGTCGAACAATAGATATAGA+AGG - chr4_1:80591973-80591992 Msa0576520:intron 35.0%
! TTTCTGTAGGCTTTAACTGT+AGG + chr4_1:80592107-80592126 None:intergenic 35.0%
AAAATGGATCTTAGTTGGAC+TGG + chr4_1:80592132-80592151 None:intergenic 35.0%
ATTCAGTTTGCATCGAAGAT+AGG - chr4_1:80591226-80591245 Msa0576520:three_prime_UTR 35.0%
TTCAGTTTGCATCGAAGATA+GGG - chr4_1:80591227-80591246 Msa0576520:three_prime_UTR 35.0%
!! AACACTTCGTCTCTGAAATT+AGG + chr4_1:80591553-80591572 None:intergenic 35.0%
! TTGAGTCTCTAACTCACTTT+TGG + chr4_1:80591585-80591604 None:intergenic 35.0%
AAGTGAGTTAGAGACTCAAA+AGG - chr4_1:80591586-80591605 Msa0576520:CDS 35.0%
! TTTCAGTGCGATTGATGATT+GGG + chr4_1:80591613-80591632 None:intergenic 35.0%
!! TTTTCAGTGCGATTGATGAT+TGG + chr4_1:80591614-80591633 None:intergenic 35.0%
!! TTTCTGAAACAAGACTGCAA+GGG + chr4_1:80591934-80591953 None:intergenic 35.0%
!!! TTTTCTGAAACAAGACTGCA+AGG + chr4_1:80591935-80591954 None:intergenic 35.0%
!! TGCAGTCTTGTTTCAGAAAA+TGG - chr4_1:80591935-80591954 Msa0576520:intron 35.0%
!! GCAGTCTTGTTTCAGAAAAT+GGG - chr4_1:80591936-80591955 Msa0576520:intron 35.0%
!! CAGTCTTGTTTCAGAAAATG+GGG - chr4_1:80591937-80591956 Msa0576520:intron 35.0%
GGTCGAACAATAGATATAGA+AGG - chr4_1:80591973-80591992 Msa0576520:intron 35.0%
! TTTCTGTAGGCTTTAACTGT+AGG + chr4_1:80592107-80592126 None:intergenic 35.0%
AAAATGGATCTTAGTTGGAC+TGG + chr4_1:80592132-80592151 None:intergenic 35.0%
TTTGCATCGAAGATAGGGAA+TGG - chr4_1:80591232-80591251 Msa0576520:three_prime_UTR 40.0%
GCAAATTACAGCAGATCTCA+TGG - chr4_1:80591294-80591313 Msa0576520:three_prime_UTR 40.0%
AATCACAAGTTAGTAGCAGC+TGG + chr4_1:80591351-80591370 None:intergenic 40.0%
TTGACACAGCAATAGAAAGC+AGG + chr4_1:80591385-80591404 None:intergenic 40.0%
CACTGAAAATACCAGTACTG+AGG - chr4_1:80591625-80591644 Msa0576520:CDS 40.0%
!! TTGAAGTGATGGTGTTGCAA+GGG + chr4_1:80591733-80591752 None:intergenic 40.0%
!! TTGAAGTGATGGTGTTGCAA+GGG + chr4_1:80591733-80591752 None:intergenic 40.0%
!! TTGAAGTGATGGTGTTGCAT+GGG + chr4_1:80591799-80591818 None:intergenic 40.0%
!! TTGAAGTGATGGTGTTGCAT+GGG + chr4_1:80591799-80591818 None:intergenic 40.0%
!! TTGAAGTGATGGTGTTGCAT+GGG + chr4_1:80591799-80591818 None:intergenic 40.0%
!! CAAAGGTGTTGTTGAAGTGA+CGG + chr4_1:80591909-80591928 None:intergenic 40.0%
AAATGGGGAAATTAAGCCGA+AGG - chr4_1:80591952-80591971 Msa0576520:intron 40.0%
AGTCTAACTTAGATGACCAG+AGG - chr4_1:80591996-80592015 Msa0576520:intron 40.0%
TGTATGTGTGCTCTCTGTAA+TGG - chr4_1:80592049-80592068 Msa0576520:intron 40.0%
!!! AGTTGGACTGGTTTTTCTGT+AGG + chr4_1:80592120-80592139 None:intergenic 40.0%
TTTGCATCGAAGATAGGGAA+TGG - chr4_1:80591232-80591251 Msa0576520:three_prime_UTR 40.0%
GCAAATTACAGCAGATCTCA+TGG - chr4_1:80591294-80591313 Msa0576520:three_prime_UTR 40.0%
AATCACAAGTTAGTAGCAGC+TGG + chr4_1:80591351-80591370 None:intergenic 40.0%
TTGACACAGCAATAGAAAGC+AGG + chr4_1:80591385-80591404 None:intergenic 40.0%
CACTGAAAATACCAGTACTG+AGG - chr4_1:80591625-80591644 Msa0576520:CDS 40.0%
!! TTGAAGTGATGGTGTTGCAA+GGG + chr4_1:80591733-80591752 None:intergenic 40.0%
!! TTGAAGTGATGGTGTTGCAA+GGG + chr4_1:80591733-80591752 None:intergenic 40.0%
!! TTGAAGTGATGGTGTTGCAT+GGG + chr4_1:80591799-80591818 None:intergenic 40.0%
!! TTGAAGTGATGGTGTTGCAT+GGG + chr4_1:80591799-80591818 None:intergenic 40.0%
!! TTGAAGTGATGGTGTTGCAT+GGG + chr4_1:80591799-80591818 None:intergenic 40.0%
!! CAAAGGTGTTGTTGAAGTGA+CGG + chr4_1:80591909-80591928 None:intergenic 40.0%
AAATGGGGAAATTAAGCCGA+AGG - chr4_1:80591952-80591971 Msa0576520:intron 40.0%
AGTCTAACTTAGATGACCAG+AGG - chr4_1:80591996-80592015 Msa0576520:intron 40.0%
TGTATGTGTGCTCTCTGTAA+TGG - chr4_1:80592049-80592068 Msa0576520:intron 40.0%
!!! AGTTGGACTGGTTTTTCTGT+AGG + chr4_1:80592120-80592139 None:intergenic 40.0%
GGATCCACCTGTAATAACGT+CGG - chr4_1:80591668-80591687 Msa0576520:CDS 45.0%
TTTGCCGACGTTATTACAGG+TGG + chr4_1:80591675-80591694 None:intergenic 45.0%
TGCTTTGCCGACGTTATTAC+AGG + chr4_1:80591678-80591697 None:intergenic 45.0%
AATAACGTCGGCAAAGCAGT+TGG - chr4_1:80591680-80591699 Msa0576520:CDS 45.0%
!! GTTGAAGTGATGGTGTTGCA+AGG + chr4_1:80591734-80591753 None:intergenic 45.0%
CACCATCACTTCAACACCTA+TGG - chr4_1:80591739-80591758 Msa0576520:CDS 45.0%
!! GACCATAGGTGTTGAAGTGA+TGG + chr4_1:80591744-80591763 None:intergenic 45.0%
!! GTTGAAGTGATGGTGTTGCA+AGG + chr4_1:80591734-80591753 None:intergenic 45.0%
CACCATCACTTCAACACCTT+TGG - chr4_1:80591772-80591791 Msa0576520:CDS 45.0%
!! GACCAAAGGTGTTGAAGTGA+TGG + chr4_1:80591777-80591796 None:intergenic 45.0%
!! GTTGAAGTGATGGTGTTGCA+TGG + chr4_1:80591734-80591753 None:intergenic 45.0%
CACCATCACTTCAACACCTT+TGG - chr4_1:80591772-80591791 Msa0576520:CDS 45.0%
!! GACCAAAGGTGTTGAAGTGA+TGG + chr4_1:80591777-80591796 None:intergenic 45.0%
!! GTTGAAGTGATGGTGTTGCA+TGG + chr4_1:80591734-80591753 None:intergenic 45.0%
CACCATCACTTCAACACCTT+TGG - chr4_1:80591772-80591791 Msa0576520:CDS 45.0%
!! GACCAAAGGTGTTGAAGTGA+TGG + chr4_1:80591777-80591796 None:intergenic 45.0%
!! GTTGAAGTGATGGTGTTGCA+TGG + chr4_1:80591734-80591753 None:intergenic 45.0%
!! TTGAAGTGACGGTGTTGCAA+GGG + chr4_1:80591898-80591917 None:intergenic 45.0%
CGTCACTTCAACAACACCTT+TGG - chr4_1:80591907-80591926 Msa0576520:intron 45.0%
TTACTTCCAAACGGTACCTC+TGG + chr4_1:80592015-80592034 None:intergenic 45.0%
GGATCCACCTGTAATAACGT+CGG - chr4_1:80591668-80591687 Msa0576520:CDS 45.0%
TTTGCCGACGTTATTACAGG+TGG + chr4_1:80591675-80591694 None:intergenic 45.0%
TGCTTTGCCGACGTTATTAC+AGG + chr4_1:80591678-80591697 None:intergenic 45.0%
AATAACGTCGGCAAAGCAGT+TGG - chr4_1:80591680-80591699 Msa0576520:CDS 45.0%
!! GTTGAAGTGATGGTGTTGCA+AGG + chr4_1:80591734-80591753 None:intergenic 45.0%
CACCATCACTTCAACACCTA+TGG - chr4_1:80591739-80591758 Msa0576520:CDS 45.0%
!! GACCATAGGTGTTGAAGTGA+TGG + chr4_1:80591744-80591763 None:intergenic 45.0%
!! GTTGAAGTGATGGTGTTGCA+AGG + chr4_1:80591734-80591753 None:intergenic 45.0%
CACCATCACTTCAACACCTT+TGG - chr4_1:80591772-80591791 Msa0576520:CDS 45.0%
!! GACCAAAGGTGTTGAAGTGA+TGG + chr4_1:80591777-80591796 None:intergenic 45.0%
!! GTTGAAGTGATGGTGTTGCA+TGG + chr4_1:80591734-80591753 None:intergenic 45.0%
CACCATCACTTCAACACCTT+TGG - chr4_1:80591772-80591791 Msa0576520:CDS 45.0%
!! GACCAAAGGTGTTGAAGTGA+TGG + chr4_1:80591777-80591796 None:intergenic 45.0%
!! GTTGAAGTGATGGTGTTGCA+TGG + chr4_1:80591734-80591753 None:intergenic 45.0%
CACCATCACTTCAACACCTT+TGG - chr4_1:80591772-80591791 Msa0576520:CDS 45.0%
!! GACCAAAGGTGTTGAAGTGA+TGG + chr4_1:80591777-80591796 None:intergenic 45.0%
!! GTTGAAGTGATGGTGTTGCA+TGG + chr4_1:80591734-80591753 None:intergenic 45.0%
!! TTGAAGTGACGGTGTTGCAA+GGG + chr4_1:80591898-80591917 None:intergenic 45.0%
CGTCACTTCAACAACACCTT+TGG - chr4_1:80591907-80591926 Msa0576520:intron 45.0%
TTACTTCCAAACGGTACCTC+TGG + chr4_1:80592015-80592034 None:intergenic 45.0%
CACCATCACTTCAGCACCTA+TGG - chr4_1:80591706-80591725 Msa0576520:CDS 50.0%
!! GACCATAGGTGCTGAAGTGA+TGG + chr4_1:80591711-80591730 None:intergenic 50.0%
!! ATGGTGTTGCAAGGGACCAT+AGG + chr4_1:80591725-80591744 None:intergenic 50.0%
!! ATGGTGTTGCAAGGGACCAT+AGG + chr4_1:80591725-80591744 None:intergenic 50.0%
!! ATGGTGTTGCATGGGACCAA+AGG + chr4_1:80591791-80591810 None:intergenic 50.0%
!! ATGGTGTTGCATGGGACCAA+AGG + chr4_1:80591791-80591810 None:intergenic 50.0%
!! ATGGTGTTGCATGGGACCAA+AGG + chr4_1:80591791-80591810 None:intergenic 50.0%
CACCATCACTTCAACGCCTT+TGG - chr4_1:80591871-80591890 Msa0576520:intron 50.0%
!! GACCAAAGGCGTTGAAGTGA+TGG + chr4_1:80591876-80591895 None:intergenic 50.0%
!! GTTGAAGTGACGGTGTTGCA+AGG + chr4_1:80591899-80591918 None:intergenic 50.0%
ACAAGACTGCAAGGGACCAA+AGG + chr4_1:80591926-80591945 None:intergenic 50.0%
!! AGATGACCAGAGGTACCGTT+TGG - chr4_1:80592006-80592025 Msa0576520:intron 50.0%
CACCATCACTTCAGCACCTA+TGG - chr4_1:80591706-80591725 Msa0576520:CDS 50.0%
!! GACCATAGGTGCTGAAGTGA+TGG + chr4_1:80591711-80591730 None:intergenic 50.0%
!! ATGGTGTTGCAAGGGACCAT+AGG + chr4_1:80591725-80591744 None:intergenic 50.0%
!! ATGGTGTTGCAAGGGACCAT+AGG + chr4_1:80591725-80591744 None:intergenic 50.0%
!! ATGGTGTTGCATGGGACCAA+AGG + chr4_1:80591791-80591810 None:intergenic 50.0%
!! ATGGTGTTGCATGGGACCAA+AGG + chr4_1:80591791-80591810 None:intergenic 50.0%
!! ATGGTGTTGCATGGGACCAA+AGG + chr4_1:80591791-80591810 None:intergenic 50.0%
CACCATCACTTCAACGCCTT+TGG - chr4_1:80591871-80591890 Msa0576520:intron 50.0%
!! GACCAAAGGCGTTGAAGTGA+TGG + chr4_1:80591876-80591895 None:intergenic 50.0%
!! GTTGAAGTGACGGTGTTGCA+AGG + chr4_1:80591899-80591918 None:intergenic 50.0%
ACAAGACTGCAAGGGACCAA+AGG + chr4_1:80591926-80591945 None:intergenic 50.0%
!! AGATGACCAGAGGTACCGTT+TGG - chr4_1:80592006-80592025 Msa0576520:intron 50.0%
!! ACGGTGTTGCAAGGGACCAA+AGG + chr4_1:80591890-80591909 None:intergenic 55.0%
!! ACGGTGTTGCAAGGGACCAA+AGG + chr4_1:80591890-80591909 None:intergenic 55.0%
CTGTTGCGCTGCCTCAGTAC+TGG + chr4_1:80591639-80591658 None:intergenic 60.0%
ACTGAGGCAGCGCAACAGCA+AGG - chr4_1:80591641-80591660 Msa0576520:CDS 60.0%
GCAGCGCAACAGCAAGGACT+GGG - chr4_1:80591647-80591666 Msa0576520:CDS 60.0%
CTGTTGCGCTGCCTCAGTAC+TGG + chr4_1:80591639-80591658 None:intergenic 60.0%
ACTGAGGCAGCGCAACAGCA+AGG - chr4_1:80591641-80591660 Msa0576520:CDS 60.0%
GCAGCGCAACAGCAAGGACT+GGG - chr4_1:80591647-80591666 Msa0576520:CDS 60.0%
GGCAGCGCAACAGCAAGGAC+TGG - chr4_1:80591646-80591665 Msa0576520:CDS 65.0%
GGCAGCGCAACAGCAAGGAC+TGG - chr4_1:80591646-80591665 Msa0576520:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 80591169 80592216 80591169 ID=Msa0576520;Name=Msa0576520
chr4_1 mRNA 80591169 80592216 80591169 ID=Msa0576520-mRNA-1;Parent=Msa0576520;Name=Msa0576520-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0.5|0.5|0|0|2|231|169
chr4_1 exon 80591169 80591824 80591169 ID=Msa0576520-mRNA-1:exon:28932;Parent=Msa0576520-mRNA-1
chr4_1 exon 80592132 80592216 80592132 ID=Msa0576520-mRNA-1:exon:28931;Parent=Msa0576520-mRNA-1
chr4_1 CDS 80592132 80592216 80592132 ID=Msa0576520-mRNA-1:cds;Parent=Msa0576520-mRNA-1
chr4_1 CDS 80591400 80591824 80591400 ID=Msa0576520-mRNA-1:cds;Parent=Msa0576520-mRNA-1
chr4_1 three_prime_UTR 80591169 80591399 80591169 ID=Msa0576520-mRNA-1:three_prime_utr;Parent=Msa0576520-mRNA-1
Gene Sequence

>Msa0576520

ATGCATTCAACTTTCAATTTAGAGAACTGTGATACTGGATACCAATTTTCAAAAAATATTCAGTTTGCATCGAAGATAGGGAATGAGACGAAGTGTTCTTCATTAGCCAAAAGTGAGTTAGAGACTCAAAAGGACCCAATCATCAATCGCACTGAAAATACCAGTACTGAGGCAGCGCAACAGCAAGGACTGGGATCCACCTGTAATAACGTCGGCAAAGCAGTTGGCAACACCATCACTTCAGCACCTATGGTCCCTTGCAACACCATCACTTCAACACCTATGGTCCCTTGCAACACCATCACTTCAACACCTTTGGTCCCATGCAACACCATCACTTCAACACCTTTGGTCCCATGCAACACCATCACTTCAACACCTTTGGTCCCATGCAACACCATCACTTCAACGCCTTTGGTCCCTTGCAACACCGTCACTTCAACAACACCTTTGGTCCCTTGCAGTCTTGTTTCAGAAAATGGGGAAATTAAGCCGAAGGTCGAACAATAG
Protein sequence

>Msa0576520

MHSTFNLENCDTGYQFSKNIQFASKIGNETKCSSLAKSELETQKDPIINRTENTSTEAAQQQGLGSTCNNVGKAVGNTITSAPMVPCNTITSTPMVPCNTITSTPLVPCNTITSTPLVPCNTITSTPLVPCNTITSTPLVPCNTVTSTTPLVPCSLVSENGEIKPKVEQ*