AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0576610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576610 MtrunA17_Chr8g0390711 94.697 132 7 0 1 132 1 132 4.46e-90 257
Msa0576610 MtrunA17_Chr6g0485081 80.153 131 25 1 1 131 1 130 2.21e-75 220
Msa0576610 MtrunA17_Chr1g0182101 41.176 119 57 3 17 131 141 250 1.44e-23 92.0
Msa0576610 MtrunA17_Chr4g0050571 53.030 66 31 0 66 131 101 166 2.86e-23 89.0
Msa0576610 MtrunA17_Chr4g0030661 56.061 66 29 0 64 129 210 275 1.11e-21 87.8
Msa0576610 MtrunA17_Chr7g0263491 50.000 64 32 0 66 129 209 272 9.52e-20 82.4
Msa0576610 MtrunA17_Chr1g0198341 43.284 67 37 1 66 131 4 70 4.83e-12 57.8
Msa0576610 MtrunA17_Chr7g0218981 40.000 65 38 1 66 129 4 68 1.95e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576610 AT3G24450.1 56.835 139 52 4 1 131 1 139 1.06e-43 140
Msa0576610 AT5G02600.2 60.938 64 25 0 66 129 250 313 3.18e-23 92.4
Msa0576610 AT5G02600.1 60.938 64 25 0 66 129 250 313 3.18e-23 92.4
Msa0576610 AT2G37390.1 50.000 76 38 0 56 131 171 246 6.13e-23 90.5
Msa0576610 AT2G37390.2 54.545 66 30 0 66 131 180 245 9.89e-23 90.1
Msa0576610 AT3G53530.1 56.716 67 27 1 66 130 168 234 8.86e-22 87.4
Msa0576610 AT2G28660.1 57.812 64 27 0 66 129 183 246 1.91e-20 84.3
Msa0576610 AT3G53530.2 55.882 68 27 2 66 130 168 235 5.48e-20 82.8
Msa0576610 AT1G66240.1 40.299 67 39 1 66 131 33 99 4.16e-12 58.9
Msa0576610 AT3G56240.3 40.000 65 38 1 66 129 3 67 5.99e-11 56.2
Msa0576610 AT3G56240.1 40.000 65 38 1 66 129 3 67 5.99e-11 56.2

Find 47 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTCATTATCAAAATTTA+TGG 0.091750 4_1:-80681172 None:intergenic
AATCATGCACATTTGCTTTA+TGG 0.118411 4_1:+80682594 Msa0576610:three_prime_UTR
GCCAATGACAGCAACCATTT+TGG 0.233480 4_1:-80682255 None:intergenic
ATTGGTGACATAACTATAAA+TGG 0.241539 4_1:-80680798 None:intergenic
GATTCCTTCAACTCACATAA+TGG 0.306207 4_1:+80681080 Msa0576610:exon
TAGTTGATCTTTCTCCTTCT+CGG 0.325138 4_1:+80682568 Msa0576610:three_prime_UTR
GTTCTAGCAACTCTCTTTGC+AGG 0.337096 4_1:+80682513 Msa0576610:three_prime_UTR
TGTCTCCATTGGATTTAGTT+TGG 0.342390 4_1:+80680831 Msa0576610:five_prime_UTR
TGCAGGAAAGAAGTTGAAAC+AGG 0.355505 4_1:+80682530 Msa0576610:three_prime_UTR
ATTCCTTCAACTCACATAAT+GGG 0.359263 4_1:+80681081 Msa0576610:exon
CACAACACTTCCTTATGTTA+AGG 0.360864 4_1:-80681023 None:intergenic
ATCTCTGTCACTTGCAATCA+AGG 0.372146 4_1:-80681205 None:intergenic
ACCAAAATGGTTGCTGTCAT+TGG 0.375586 4_1:+80682254 Msa0576610:CDS
TTCCCCATTATGTGAGTTGA+AGG 0.395303 4_1:-80681084 None:intergenic
GTTTGGTCAATGTAATTTAG+TGG 0.403129 4_1:+80680848 Msa0576610:five_prime_UTR
ATAGTTATGTCACCAATGTT+TGG 0.415119 4_1:+80680804 Msa0576610:five_prime_UTR
AACAGGAGTGAGCTCATATA+AGG 0.416871 4_1:+80682220 Msa0576610:intron
CGATTGAAGGATGTTGTTGC+TGG 0.427133 4_1:+80681240 Msa0576610:CDS
GGTAGATCTAGACACCAAAA+TGG 0.437295 4_1:+80682241 Msa0576610:CDS
TGTTTGGTATCTGTCTCCAT+TGG 0.444454 4_1:+80680820 Msa0576610:five_prime_UTR
GGGGAAACTTGGTAAAATGT+TGG 0.462441 4_1:+80681101 Msa0576610:CDS
AAACAGAAATGTATTAAAAG+AGG 0.466049 4_1:-80680772 None:intergenic
ACTCACATAATGGGGAAACT+TGG 0.481047 4_1:+80681090 Msa0576610:exon
TATGCAGATAGTAATATTGA+AGG 0.490687 4_1:+80681818 Msa0576610:intron
ATTAATAGAGAGATTAGAAA+AGG 0.495760 4_1:-80680726 None:intergenic
TGCTAGAACATGCATTGCAT+GGG 0.514201 4_1:-80682499 None:intergenic
ATGACAAAATTTAATCTCAC+TGG 0.518273 4_1:+80680879 Msa0576610:five_prime_UTR
ACACTTTGCAACACTTCGAA+TGG 0.529196 4_1:-80682287 None:intergenic
CTTTATGGTGCAGAGGCTGT+AGG 0.534556 4_1:+80682609 Msa0576610:three_prime_UTR
TTATTCTAGATAAATTCCAT+TGG 0.541838 4_1:+80680917 Msa0576610:five_prime_UTR
TTGCTAGAACATGCATTGCA+TGG 0.544380 4_1:-80682500 None:intergenic
AAACATATATCAAAGCTTGA+AGG 0.550802 4_1:+80681879 Msa0576610:CDS
TGCACAGCCATGACAATGCA+TGG 0.563796 4_1:-80681844 None:intergenic
AGTGTTGCAAAGTGTATCAA+AGG 0.566152 4_1:+80682295 Msa0576610:CDS
ATTGACCAAACTAAATCCAA+TGG 0.577521 4_1:-80680836 None:intergenic
TCTCTGTCACTTGCAATCAA+GGG 0.585982 4_1:-80681204 None:intergenic
TTGGTGGAATCTGCTAATGC+CGG 0.598406 4_1:-80682393 None:intergenic
TCTGCATTGCTAAACTCCAC+CGG 0.607232 4_1:+80682374 Msa0576610:three_prime_UTR
TTCCTTCAACTCACATAATG+GGG 0.610996 4_1:+80681082 Msa0576610:exon
GTGGAATCTGCTAATGCCGG+TGG 0.615330 4_1:-80682390 None:intergenic
ACATTTGCTTTATGGTGCAG+AGG 0.619699 4_1:+80682602 Msa0576610:three_prime_UTR
TGTGCATGATTCATCCGAGA+AGG 0.632281 4_1:-80682582 None:intergenic
TGTTGCTGGAAAGCAAACAT+TGG 0.652091 4_1:+80681254 Msa0576610:CDS
AAGGTATCCATGCATTGTCA+TGG 0.661899 4_1:+80681837 Msa0576610:CDS
TGGAGACAGATACCAAACAT+TGG 0.669694 4_1:-80680816 None:intergenic
GCAGGAAAGAAGTTGAAACA+GGG 0.672653 4_1:+80682531 Msa0576610:three_prime_UTR
GCTAGAACATGCATTGCATG+GGG 0.698020 4_1:-80682498 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTCATTATCAAAATTTA+TGG - chr4_1:80681175-80681194 None:intergenic 15.0%
!!! ATTTTAAAATATAAGCAATC+TGG - chr4_1:80681370-80681389 None:intergenic 15.0%
!! ACAAATACAAACAAAATTAA+AGG - chr4_1:80681920-80681939 None:intergenic 15.0%
!!! CATCTTTTAATTAGTAAATT+TGG + chr4_1:80682672-80682691 Msa0576610:three_prime_UTR 15.0%
!! AAACTCATTATCAAAATTTA+TGG - chr4_1:80681175-80681194 None:intergenic 15.0%
!!! ATTTTAAAATATAAGCAATC+TGG - chr4_1:80681370-80681389 None:intergenic 15.0%
!! ACAAATACAAACAAAATTAA+AGG - chr4_1:80681920-80681939 None:intergenic 15.0%
!! ATTAATAGAGAGATTAGAAA+AGG - chr4_1:80680729-80680748 None:intergenic 20.0%
!! AAACAGAAATGTATTAAAAG+AGG - chr4_1:80680775-80680794 None:intergenic 20.0%
!! TTATTCTAGATAAATTCCAT+TGG + chr4_1:80680917-80680936 Msa0576610:five_prime_UTR 20.0%
!!! TTAAGGTGAAAATTTTATTG+GGG - chr4_1:80680966-80680985 None:intergenic 20.0%
!!! CTTAAGGTGAAAATTTTATT+GGG - chr4_1:80680967-80680986 None:intergenic 20.0%
!!! TTAGAATATTGATAATGTAG+AGG + chr4_1:80681628-80681647 Msa0576610:intron 20.0%
!! GATAATATATCTCATAAGAT+GGG - chr4_1:80682041-80682060 None:intergenic 20.0%
!! AGATAATATATCTCATAAGA+TGG - chr4_1:80682042-80682061 None:intergenic 20.0%
!! TAAGATATAGTATCAAAATG+TGG + chr4_1:80682135-80682154 Msa0576610:intron 20.0%
!!! ATTTTTTCTTTATAACTAGC+AGG - chr4_1:80682348-80682367 None:intergenic 20.0%
!! ATTAATAGAGAGATTAGAAA+AGG - chr4_1:80680729-80680748 None:intergenic 20.0%
!! AAACAGAAATGTATTAAAAG+AGG - chr4_1:80680775-80680794 None:intergenic 20.0%
!! TTATTCTAGATAAATTCCAT+TGG + chr4_1:80680917-80680936 Msa0576610:five_prime_UTR 20.0%
!!! TTAAGGTGAAAATTTTATTG+GGG - chr4_1:80680966-80680985 None:intergenic 20.0%
!!! CTTAAGGTGAAAATTTTATT+GGG - chr4_1:80680967-80680986 None:intergenic 20.0%
!!! TTAGAATATTGATAATGTAG+AGG + chr4_1:80681628-80681647 Msa0576610:intron 20.0%
!! GATAATATATCTCATAAGAT+GGG - chr4_1:80682041-80682060 None:intergenic 20.0%
!! AGATAATATATCTCATAAGA+TGG - chr4_1:80682042-80682061 None:intergenic 20.0%
!! TAAGATATAGTATCAAAATG+TGG + chr4_1:80682135-80682154 Msa0576610:intron 20.0%
!!! ATTTTTTCTTTATAACTAGC+AGG - chr4_1:80682348-80682367 None:intergenic 20.0%
!! ATTGGTGACATAACTATAAA+TGG - chr4_1:80680801-80680820 None:intergenic 25.0%
! ATGACAAAATTTAATCTCAC+TGG + chr4_1:80680879-80680898 Msa0576610:five_prime_UTR 25.0%
!! GCTTAAGGTGAAAATTTTAT+TGG - chr4_1:80680968-80680987 None:intergenic 25.0%
!!! TTCCTTTTTTCCTTAACATA+AGG + chr4_1:80681013-80681032 Msa0576610:five_prime_UTR 25.0%
! TTCCTTATGTTAAGGAAAAA+AGG - chr4_1:80681018-80681037 None:intergenic 25.0%
! TTAAAATATAAGCAATCTGG+TGG - chr4_1:80681367-80681386 None:intergenic 25.0%
! ATTAACATTGTGCAAGTATA+GGG - chr4_1:80681396-80681415 None:intergenic 25.0%
! TGAAATCAGATCAAGATAAA+GGG - chr4_1:80681428-80681447 None:intergenic 25.0%
! TTGAAATCAGATCAAGATAA+AGG - chr4_1:80681429-80681448 None:intergenic 25.0%
! AAGTTAAAAATGACAGAGAT+GGG - chr4_1:80681453-80681472 None:intergenic 25.0%
! TAAGTTAAAAATGACAGAGA+TGG - chr4_1:80681454-80681473 None:intergenic 25.0%
! GAACTATAATGACATCAAAT+CGG - chr4_1:80681501-80681520 None:intergenic 25.0%
! ATTCACATCTTACAATTTCA+AGG + chr4_1:80681600-80681619 Msa0576610:intron 25.0%
! TAAAATTTCAGAGATCAACT+TGG + chr4_1:80681678-80681697 Msa0576610:intron 25.0%
! TGGATATTTACTCTTCATTA+TGG + chr4_1:80681698-80681717 Msa0576610:intron 25.0%
! TATGCAGATAGTAATATTGA+AGG + chr4_1:80681818-80681837 Msa0576610:intron 25.0%
! AAACATATATCAAAGCTTGA+AGG + chr4_1:80681879-80681898 Msa0576610:CDS 25.0%
! GAAGATGAAACAAATTGTAT+TGG + chr4_1:80681980-80681999 Msa0576610:intron 25.0%
! TTAATGTGATAAAAGCAAAG+AGG + chr4_1:80682003-80682022 Msa0576610:intron 25.0%
! TTAGCCATCTTGATATATAA+AGG + chr4_1:80682061-80682080 Msa0576610:intron 25.0%
!! TGAATTTTTCATATGTGACT+TGG - chr4_1:80682087-80682106 None:intergenic 25.0%
!! ATTGGTGACATAACTATAAA+TGG - chr4_1:80680801-80680820 None:intergenic 25.0%
! ATGACAAAATTTAATCTCAC+TGG + chr4_1:80680879-80680898 Msa0576610:five_prime_UTR 25.0%
!! GCTTAAGGTGAAAATTTTAT+TGG - chr4_1:80680968-80680987 None:intergenic 25.0%
!!! TTCCTTTTTTCCTTAACATA+AGG + chr4_1:80681013-80681032 Msa0576610:five_prime_UTR 25.0%
! TTCCTTATGTTAAGGAAAAA+AGG - chr4_1:80681018-80681037 None:intergenic 25.0%
! TTAAAATATAAGCAATCTGG+TGG - chr4_1:80681367-80681386 None:intergenic 25.0%
! ATTAACATTGTGCAAGTATA+GGG - chr4_1:80681396-80681415 None:intergenic 25.0%
! TGAAATCAGATCAAGATAAA+GGG - chr4_1:80681428-80681447 None:intergenic 25.0%
! TTGAAATCAGATCAAGATAA+AGG - chr4_1:80681429-80681448 None:intergenic 25.0%
! AAGTTAAAAATGACAGAGAT+GGG - chr4_1:80681453-80681472 None:intergenic 25.0%
! TAAGTTAAAAATGACAGAGA+TGG - chr4_1:80681454-80681473 None:intergenic 25.0%
! GAACTATAATGACATCAAAT+CGG - chr4_1:80681501-80681520 None:intergenic 25.0%
! ATTCACATCTTACAATTTCA+AGG + chr4_1:80681600-80681619 Msa0576610:intron 25.0%
! TAAAATTTCAGAGATCAACT+TGG + chr4_1:80681678-80681697 Msa0576610:intron 25.0%
! TGGATATTTACTCTTCATTA+TGG + chr4_1:80681698-80681717 Msa0576610:intron 25.0%
! TATGCAGATAGTAATATTGA+AGG + chr4_1:80681818-80681837 Msa0576610:intron 25.0%
! AAACATATATCAAAGCTTGA+AGG + chr4_1:80681879-80681898 Msa0576610:CDS 25.0%
! GAAGATGAAACAAATTGTAT+TGG + chr4_1:80681980-80681999 Msa0576610:intron 25.0%
! TTAATGTGATAAAAGCAAAG+AGG + chr4_1:80682003-80682022 Msa0576610:intron 25.0%
! TTAGCCATCTTGATATATAA+AGG + chr4_1:80682061-80682080 Msa0576610:intron 25.0%
!! TGAATTTTTCATATGTGACT+TGG - chr4_1:80682087-80682106 None:intergenic 25.0%
ATAGTTATGTCACCAATGTT+TGG + chr4_1:80680804-80680823 Msa0576610:five_prime_UTR 30.0%
ATTGACCAAACTAAATCCAA+TGG - chr4_1:80680839-80680858 None:intergenic 30.0%
GTTTGGTCAATGTAATTTAG+TGG + chr4_1:80680848-80680867 Msa0576610:five_prime_UTR 30.0%
TCAAATTGCACAAAAACCAA+TGG - chr4_1:80680936-80680955 None:intergenic 30.0%
AAAAAAGGAACATCAGGTAT+GGG - chr4_1:80681003-80681022 None:intergenic 30.0%
TTAAGGAAAAAAGGAACATC+AGG - chr4_1:80681009-80681028 None:intergenic 30.0%
!!! TGTGTTTTGAGAGCATATAT+AGG + chr4_1:80681052-80681071 Msa0576610:five_prime_UTR 30.0%
ATTCCTTCAACTCACATAAT+GGG + chr4_1:80681081-80681100 Msa0576610:exon 30.0%
!! GATACATTCTGTTTTTCTTC+TGG + chr4_1:80681123-80681142 Msa0576610:CDS 30.0%
TGATCAAAAACTACGATTGA+AGG + chr4_1:80681227-80681246 Msa0576610:CDS 30.0%
GATTAACATTGTGCAAGTAT+AGG - chr4_1:80681397-80681416 None:intergenic 30.0%
AATATATCTCATAAGATGGG+AGG - chr4_1:80682038-80682057 None:intergenic 30.0%
! TTGGCCTTTATATATCAAGA+TGG - chr4_1:80682068-80682087 None:intergenic 30.0%
TATAGTATCAAAATGTGGCA+TGG + chr4_1:80682140-80682159 Msa0576610:intron 30.0%
! AGGTTAAAAATGCACAGTTT+TGG + chr4_1:80682315-80682334 Msa0576610:CDS 30.0%
! TTTTCTTTATAACTAGCAGG+TGG - chr4_1:80682345-80682364 None:intergenic 30.0%
AATCATGCACATTTGCTTTA+TGG + chr4_1:80682594-80682613 Msa0576610:three_prime_UTR 30.0%
ATAGTTATGTCACCAATGTT+TGG + chr4_1:80680804-80680823 Msa0576610:five_prime_UTR 30.0%
ATTGACCAAACTAAATCCAA+TGG - chr4_1:80680839-80680858 None:intergenic 30.0%
GTTTGGTCAATGTAATTTAG+TGG + chr4_1:80680848-80680867 Msa0576610:five_prime_UTR 30.0%
TCAAATTGCACAAAAACCAA+TGG - chr4_1:80680936-80680955 None:intergenic 30.0%
AAAAAAGGAACATCAGGTAT+GGG - chr4_1:80681003-80681022 None:intergenic 30.0%
TTAAGGAAAAAAGGAACATC+AGG - chr4_1:80681009-80681028 None:intergenic 30.0%
!!! TGTGTTTTGAGAGCATATAT+AGG + chr4_1:80681052-80681071 Msa0576610:five_prime_UTR 30.0%
ATTCCTTCAACTCACATAAT+GGG + chr4_1:80681081-80681100 Msa0576610:exon 30.0%
!! GATACATTCTGTTTTTCTTC+TGG + chr4_1:80681123-80681142 Msa0576610:CDS 30.0%
TGATCAAAAACTACGATTGA+AGG + chr4_1:80681227-80681246 Msa0576610:CDS 30.0%
GATTAACATTGTGCAAGTAT+AGG - chr4_1:80681397-80681416 None:intergenic 30.0%
AATATATCTCATAAGATGGG+AGG - chr4_1:80682038-80682057 None:intergenic 30.0%
! TTGGCCTTTATATATCAAGA+TGG - chr4_1:80682068-80682087 None:intergenic 30.0%
TATAGTATCAAAATGTGGCA+TGG + chr4_1:80682140-80682159 Msa0576610:intron 30.0%
! AGGTTAAAAATGCACAGTTT+TGG + chr4_1:80682315-80682334 Msa0576610:CDS 30.0%
! TTTTCTTTATAACTAGCAGG+TGG - chr4_1:80682345-80682364 None:intergenic 30.0%
AATCATGCACATTTGCTTTA+TGG + chr4_1:80682594-80682613 Msa0576610:three_prime_UTR 30.0%
TGTCTCCATTGGATTTAGTT+TGG + chr4_1:80680831-80680850 Msa0576610:five_prime_UTR 35.0%
GGGAAAAAAGAAGAAGCTTA+AGG - chr4_1:80680983-80681002 None:intergenic 35.0%
GAAAAAAGGAACATCAGGTA+TGG - chr4_1:80681004-80681023 None:intergenic 35.0%
CACAACACTTCCTTATGTTA+AGG - chr4_1:80681026-80681045 None:intergenic 35.0%
GATTCCTTCAACTCACATAA+TGG + chr4_1:80681080-80681099 Msa0576610:exon 35.0%
TTCCTTCAACTCACATAATG+GGG + chr4_1:80681082-80681101 Msa0576610:exon 35.0%
! GGCTTTTCAATTGAAACCTA+AGG + chr4_1:80681275-80681294 Msa0576610:CDS 35.0%
GAGAAGAAAAACTTCACCTT+AGG - chr4_1:80681294-80681313 None:intergenic 35.0%
ATAGTTCCTGCAAATACCAA+CGG + chr4_1:80681514-80681533 Msa0576610:intron 35.0%
AGTGTTGCAAAGTGTATCAA+AGG + chr4_1:80682295-80682314 Msa0576610:CDS 35.0%
!! TATGTCTACTTTTGTCTTGG+TGG - chr4_1:80682412-80682431 None:intergenic 35.0%
!! GAGTATGTCTACTTTTGTCT+TGG - chr4_1:80682415-80682434 None:intergenic 35.0%
TAGTTGATCTTTCTCCTTCT+CGG + chr4_1:80682568-80682587 Msa0576610:three_prime_UTR 35.0%
TGTCTCCATTGGATTTAGTT+TGG + chr4_1:80680831-80680850 Msa0576610:five_prime_UTR 35.0%
GGGAAAAAAGAAGAAGCTTA+AGG - chr4_1:80680983-80681002 None:intergenic 35.0%
GAAAAAAGGAACATCAGGTA+TGG - chr4_1:80681004-80681023 None:intergenic 35.0%
CACAACACTTCCTTATGTTA+AGG - chr4_1:80681026-80681045 None:intergenic 35.0%
GATTCCTTCAACTCACATAA+TGG + chr4_1:80681080-80681099 Msa0576610:exon 35.0%
TTCCTTCAACTCACATAATG+GGG + chr4_1:80681082-80681101 Msa0576610:exon 35.0%
! GGCTTTTCAATTGAAACCTA+AGG + chr4_1:80681275-80681294 Msa0576610:CDS 35.0%
GAGAAGAAAAACTTCACCTT+AGG - chr4_1:80681294-80681313 None:intergenic 35.0%
ATAGTTCCTGCAAATACCAA+CGG + chr4_1:80681514-80681533 Msa0576610:intron 35.0%
AGTGTTGCAAAGTGTATCAA+AGG + chr4_1:80682295-80682314 Msa0576610:CDS 35.0%
!! TATGTCTACTTTTGTCTTGG+TGG - chr4_1:80682412-80682431 None:intergenic 35.0%
!! GAGTATGTCTACTTTTGTCT+TGG - chr4_1:80682415-80682434 None:intergenic 35.0%
TAGTTGATCTTTCTCCTTCT+CGG + chr4_1:80682568-80682587 Msa0576610:three_prime_UTR 35.0%
TGGAGACAGATACCAAACAT+TGG - chr4_1:80680819-80680838 None:intergenic 40.0%
TGTTTGGTATCTGTCTCCAT+TGG + chr4_1:80680820-80680839 Msa0576610:five_prime_UTR 40.0%
! TTCCCCATTATGTGAGTTGA+AGG - chr4_1:80681087-80681106 None:intergenic 40.0%
! ACTCACATAATGGGGAAACT+TGG + chr4_1:80681090-80681109 Msa0576610:exon 40.0%
GGGGAAACTTGGTAAAATGT+TGG + chr4_1:80681101-80681120 Msa0576610:CDS 40.0%
! TCTCTGTCACTTGCAATCAA+GGG - chr4_1:80681207-80681226 None:intergenic 40.0%
! ATCTCTGTCACTTGCAATCA+AGG - chr4_1:80681208-80681227 None:intergenic 40.0%
TGTTGCTGGAAAGCAAACAT+TGG + chr4_1:80681254-80681273 Msa0576610:CDS 40.0%
! AAGGTATCCATGCATTGTCA+TGG + chr4_1:80681837-80681856 Msa0576610:CDS 40.0%
AATGTGGCATGGTATAGACA+TGG + chr4_1:80682151-80682170 Msa0576610:intron 40.0%
ATGTGGCATGGTATAGACAT+GGG + chr4_1:80682152-80682171 Msa0576610:intron 40.0%
GACTCTTTGCACTTATGAAC+AGG + chr4_1:80682203-80682222 Msa0576610:intron 40.0%
AACAGGAGTGAGCTCATATA+AGG + chr4_1:80682220-80682239 Msa0576610:intron 40.0%
GGTAGATCTAGACACCAAAA+TGG + chr4_1:80682241-80682260 Msa0576610:CDS 40.0%
ACCAAAATGGTTGCTGTCAT+TGG + chr4_1:80682254-80682273 Msa0576610:CDS 40.0%
ACACTTTGCAACACTTCGAA+TGG - chr4_1:80682290-80682309 None:intergenic 40.0%
TGCTAGAACATGCATTGCAT+GGG - chr4_1:80682502-80682521 None:intergenic 40.0%
TTGCTAGAACATGCATTGCA+TGG - chr4_1:80682503-80682522 None:intergenic 40.0%
TGCAGGAAAGAAGTTGAAAC+AGG + chr4_1:80682530-80682549 Msa0576610:three_prime_UTR 40.0%
GCAGGAAAGAAGTTGAAACA+GGG + chr4_1:80682531-80682550 Msa0576610:three_prime_UTR 40.0%
ACATTTGCTTTATGGTGCAG+AGG + chr4_1:80682602-80682621 Msa0576610:three_prime_UTR 40.0%
TGGAGACAGATACCAAACAT+TGG - chr4_1:80680819-80680838 None:intergenic 40.0%
TGTTTGGTATCTGTCTCCAT+TGG + chr4_1:80680820-80680839 Msa0576610:five_prime_UTR 40.0%
! TTCCCCATTATGTGAGTTGA+AGG - chr4_1:80681087-80681106 None:intergenic 40.0%
! ACTCACATAATGGGGAAACT+TGG + chr4_1:80681090-80681109 Msa0576610:exon 40.0%
GGGGAAACTTGGTAAAATGT+TGG + chr4_1:80681101-80681120 Msa0576610:CDS 40.0%
! TCTCTGTCACTTGCAATCAA+GGG - chr4_1:80681207-80681226 None:intergenic 40.0%
! ATCTCTGTCACTTGCAATCA+AGG - chr4_1:80681208-80681227 None:intergenic 40.0%
TGTTGCTGGAAAGCAAACAT+TGG + chr4_1:80681254-80681273 Msa0576610:CDS 40.0%
! AAGGTATCCATGCATTGTCA+TGG + chr4_1:80681837-80681856 Msa0576610:CDS 40.0%
AATGTGGCATGGTATAGACA+TGG + chr4_1:80682151-80682170 Msa0576610:intron 40.0%
ATGTGGCATGGTATAGACAT+GGG + chr4_1:80682152-80682171 Msa0576610:intron 40.0%
GACTCTTTGCACTTATGAAC+AGG + chr4_1:80682203-80682222 Msa0576610:intron 40.0%
AACAGGAGTGAGCTCATATA+AGG + chr4_1:80682220-80682239 Msa0576610:intron 40.0%
GGTAGATCTAGACACCAAAA+TGG + chr4_1:80682241-80682260 Msa0576610:CDS 40.0%
ACCAAAATGGTTGCTGTCAT+TGG + chr4_1:80682254-80682273 Msa0576610:CDS 40.0%
ACACTTTGCAACACTTCGAA+TGG - chr4_1:80682290-80682309 None:intergenic 40.0%
TGCTAGAACATGCATTGCAT+GGG - chr4_1:80682502-80682521 None:intergenic 40.0%
TTGCTAGAACATGCATTGCA+TGG - chr4_1:80682503-80682522 None:intergenic 40.0%
TGCAGGAAAGAAGTTGAAAC+AGG + chr4_1:80682530-80682549 Msa0576610:three_prime_UTR 40.0%
GCAGGAAAGAAGTTGAAACA+GGG + chr4_1:80682531-80682550 Msa0576610:three_prime_UTR 40.0%
ACATTTGCTTTATGGTGCAG+AGG + chr4_1:80682602-80682621 Msa0576610:three_prime_UTR 40.0%
CGATTGAAGGATGTTGTTGC+TGG + chr4_1:80681240-80681259 Msa0576610:CDS 45.0%
TTCCTGCAAATACCAACGGA+AGG + chr4_1:80681518-80681537 Msa0576610:intron 45.0%
! TCTCATAAGATGGGAGGTGT+GGG - chr4_1:80682032-80682051 None:intergenic 45.0%
ATCTCATAAGATGGGAGGTG+TGG - chr4_1:80682033-80682052 None:intergenic 45.0%
TGTGGCATGGTATAGACATG+GGG + chr4_1:80682153-80682172 Msa0576610:intron 45.0%
! GCCAATGACAGCAACCATTT+TGG - chr4_1:80682258-80682277 None:intergenic 45.0%
TCTGCATTGCTAAACTCCAC+CGG + chr4_1:80682374-80682393 Msa0576610:three_prime_UTR 45.0%
TTGGTGGAATCTGCTAATGC+CGG - chr4_1:80682396-80682415 None:intergenic 45.0%
GCTAGAACATGCATTGCATG+GGG - chr4_1:80682501-80682520 None:intergenic 45.0%
GTTCTAGCAACTCTCTTTGC+AGG + chr4_1:80682513-80682532 Msa0576610:three_prime_UTR 45.0%
TGTGCATGATTCATCCGAGA+AGG - chr4_1:80682585-80682604 None:intergenic 45.0%
CGATTGAAGGATGTTGTTGC+TGG + chr4_1:80681240-80681259 Msa0576610:CDS 45.0%
TTCCTGCAAATACCAACGGA+AGG + chr4_1:80681518-80681537 Msa0576610:intron 45.0%
! TCTCATAAGATGGGAGGTGT+GGG - chr4_1:80682032-80682051 None:intergenic 45.0%
ATCTCATAAGATGGGAGGTG+TGG - chr4_1:80682033-80682052 None:intergenic 45.0%
TGTGGCATGGTATAGACATG+GGG + chr4_1:80682153-80682172 Msa0576610:intron 45.0%
! GCCAATGACAGCAACCATTT+TGG - chr4_1:80682258-80682277 None:intergenic 45.0%
TCTGCATTGCTAAACTCCAC+CGG + chr4_1:80682374-80682393 Msa0576610:three_prime_UTR 45.0%
TTGGTGGAATCTGCTAATGC+CGG - chr4_1:80682396-80682415 None:intergenic 45.0%
GCTAGAACATGCATTGCATG+GGG - chr4_1:80682501-80682520 None:intergenic 45.0%
GTTCTAGCAACTCTCTTTGC+AGG + chr4_1:80682513-80682532 Msa0576610:three_prime_UTR 45.0%
TGTGCATGATTCATCCGAGA+AGG - chr4_1:80682585-80682604 None:intergenic 45.0%
GTCCTTCCGTTGGTATTTGC+AGG - chr4_1:80681523-80681542 None:intergenic 50.0%
AGTCAAGTTCGTCCTTCCGT+TGG - chr4_1:80681533-80681552 None:intergenic 50.0%
TGCACAGCCATGACAATGCA+TGG - chr4_1:80681847-80681866 None:intergenic 50.0%
CTTTATGGTGCAGAGGCTGT+AGG + chr4_1:80682609-80682628 Msa0576610:three_prime_UTR 50.0%
GTCCTTCCGTTGGTATTTGC+AGG - chr4_1:80681523-80681542 None:intergenic 50.0%
AGTCAAGTTCGTCCTTCCGT+TGG - chr4_1:80681533-80681552 None:intergenic 50.0%
TGCACAGCCATGACAATGCA+TGG - chr4_1:80681847-80681866 None:intergenic 50.0%
CTTTATGGTGCAGAGGCTGT+AGG + chr4_1:80682609-80682628 Msa0576610:three_prime_UTR 50.0%
GTGGAATCTGCTAATGCCGG+TGG - chr4_1:80682393-80682412 None:intergenic 55.0%
GTGGAATCTGCTAATGCCGG+TGG - chr4_1:80682393-80682412 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 80680723 80682694 80680723 ID=Msa0576610;Name=Msa0576610
chr4_1 mRNA 80680723 80682694 80680723 ID=Msa0576610-mRNA-1;Parent=Msa0576610;Name=Msa0576610-mRNA-1;_AED=0.01;_eAED=0.01;_QI=376|1|1|1|1|1|3|342|133
chr4_1 exon 80680723 80681296 80680723 ID=Msa0576610-mRNA-1:exon:28864;Parent=Msa0576610-mRNA-1
chr4_1 exon 80681825 80681900 80681825 ID=Msa0576610-mRNA-1:exon:28865;Parent=Msa0576610-mRNA-1
chr4_1 exon 80682225 80682694 80682225 ID=Msa0576610-mRNA-1:exon:28866;Parent=Msa0576610-mRNA-1
chr4_1 five_prime_UTR 80680723 80681098 80680723 ID=Msa0576610-mRNA-1:five_prime_utr;Parent=Msa0576610-mRNA-1
chr4_1 CDS 80681099 80681296 80681099 ID=Msa0576610-mRNA-1:cds;Parent=Msa0576610-mRNA-1
chr4_1 CDS 80681825 80681900 80681825 ID=Msa0576610-mRNA-1:cds;Parent=Msa0576610-mRNA-1
chr4_1 CDS 80682225 80682352 80682225 ID=Msa0576610-mRNA-1:cds;Parent=Msa0576610-mRNA-1
chr4_1 three_prime_UTR 80682353 80682694 80682353 ID=Msa0576610-mRNA-1:three_prime_utr;Parent=Msa0576610-mRNA-1
Gene Sequence

>Msa0576610

ATGGGGAAACTTGGTAAAATGTTGGATACATTCTGTTTTTCTTCTGGTTCAAACTCATGCTTCTGTCTAAATTCCATAAATTTTGATAATGAGTTTGAGAGTAAGCCCTTGATTGCAAGTGACAGAGATGATCAAAAACTACGATTGAAGGATGTTGTTGCTGGAAAGCAAACATTGGCTTTTCAATTGAAACCTAAGATAGTAATATTGAAGGTATCCATGCATTGTCATGGCTGTGCAAGAAAAGTTGAGAAACATATATCAAAGCTTGAAGGAGTGAGCTCATATAAGGTAGATCTAGACACCAAAATGGTTGCTGTCATTGGCGATATTCTTCCATTCGAAGTGTTGCAAAGTGTATCAAAGGTTAAAAATGCACAGTTTTGGAATTCCACCTGCTAG
Protein sequence

>Msa0576610

MGKLGKMLDTFCFSSGSNSCFCLNSINFDNEFESKPLIASDRDDQKLRLKDVVAGKQTLAFQLKPKIVILKVSMHCHGCARKVEKHISKLEGVSSYKVDLDTKMVAVIGDILPFEVLQSVSKVKNAQFWNSTC*