AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0574320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574320 MtrunA17_Chr8g0390711 94.697 132 7 0 1 132 1 132 4.46e-90 257
Msa0574320 MtrunA17_Chr6g0485081 80.153 131 25 1 1 131 1 130 2.21e-75 220
Msa0574320 MtrunA17_Chr1g0182101 41.176 119 57 3 17 131 141 250 1.44e-23 92.0
Msa0574320 MtrunA17_Chr4g0050571 53.030 66 31 0 66 131 101 166 2.86e-23 89.0
Msa0574320 MtrunA17_Chr4g0030661 56.061 66 29 0 64 129 210 275 1.11e-21 87.8
Msa0574320 MtrunA17_Chr7g0263491 50.000 64 32 0 66 129 209 272 9.52e-20 82.4
Msa0574320 MtrunA17_Chr1g0198341 43.284 67 37 1 66 131 4 70 4.83e-12 57.8
Msa0574320 MtrunA17_Chr7g0218981 40.000 65 38 1 66 129 4 68 1.95e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574320 AT3G24450.1 56.835 139 52 4 1 131 1 139 1.06e-43 140
Msa0574320 AT5G02600.2 60.938 64 25 0 66 129 250 313 3.18e-23 92.4
Msa0574320 AT5G02600.1 60.938 64 25 0 66 129 250 313 3.18e-23 92.4
Msa0574320 AT2G37390.1 50.000 76 38 0 56 131 171 246 6.13e-23 90.5
Msa0574320 AT2G37390.2 54.545 66 30 0 66 131 180 245 9.89e-23 90.1
Msa0574320 AT3G53530.1 56.716 67 27 1 66 130 168 234 8.86e-22 87.4
Msa0574320 AT2G28660.1 57.812 64 27 0 66 129 183 246 1.91e-20 84.3
Msa0574320 AT3G53530.2 55.882 68 27 2 66 130 168 235 5.48e-20 82.8
Msa0574320 AT1G66240.1 40.299 67 39 1 66 131 33 99 4.16e-12 58.9
Msa0574320 AT3G56240.3 40.000 65 38 1 66 129 3 67 5.99e-11 56.2
Msa0574320 AT3G56240.1 40.000 65 38 1 66 129 3 67 5.99e-11 56.2

Find 45 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTCATTATCAAAATTTA+TGG 0.091750 4_1:-77773317 None:intergenic
AATCATGCACATTTGCTTTA+TGG 0.118411 4_1:+77774765 Msa0574320:three_prime_UTR
GCCAATGACAGCAACCATTT+TGG 0.233480 4_1:-77774424 None:intergenic
ATTGGTGACATAACTATAAA+TGG 0.241539 4_1:-77772943 None:intergenic
GATTCCTTCAACTCACATAA+TGG 0.306207 4_1:+77773225 Msa0574320:exon
TGGTTGATCTTTCTCCTTCT+CGG 0.321091 4_1:+77774739 Msa0574320:three_prime_UTR
GTTCTAGCAACTCTCTTTGC+AGG 0.337096 4_1:+77774682 Msa0574320:three_prime_UTR
TGTCTCCATTGGATTTAGTT+TGG 0.342390 4_1:+77772976 Msa0574320:five_prime_UTR
ATTCCTTCAACTCACATAAT+GGG 0.359263 4_1:+77773226 Msa0574320:exon
CACAACACTTCCTTATGTTA+AGG 0.360864 4_1:-77773168 None:intergenic
ATCTCTGTCACTTGCAATCA+AGG 0.372146 4_1:-77773350 None:intergenic
ACCAAAATGGTTGCTGTCAT+TGG 0.375586 4_1:+77774423 Msa0574320:CDS
TTCCCCATTATGTGAGTTGA+AGG 0.395303 4_1:-77773229 None:intergenic
GTTTGGTCAATGTAATTTAG+TGG 0.403129 4_1:+77772993 Msa0574320:five_prime_UTR
ATAGTTATGTCACCAATGTT+TGG 0.415119 4_1:+77772949 Msa0574320:five_prime_UTR
AACAGGAGTGAGCTCATATA+AGG 0.418333 4_1:+77774389 Msa0574320:intron
CGATTGAAGGATGTTGTTGC+TGG 0.427133 4_1:+77773385 Msa0574320:CDS
GGTAGATCTAGACACCAAAA+TGG 0.437295 4_1:+77774410 Msa0574320:CDS
TGTTTGGTATCTGTCTCCAT+TGG 0.444454 4_1:+77772965 Msa0574320:five_prime_UTR
ATTAATAGAGAGATTAGAAA+AGG 0.455386 4_1:-77772871 None:intergenic
GGGGAAACTTGGTAAAATGT+TGG 0.462441 4_1:+77773246 Msa0574320:CDS
AAACAGAAATGTATTAAAAG+AGG 0.466049 4_1:-77772917 None:intergenic
ACTCACATAATGGGGAAACT+TGG 0.481047 4_1:+77773235 Msa0574320:exon
TATGCAGATAGTAATATTGA+AGG 0.490687 4_1:+77773983 Msa0574320:intron
ATGACAAAATTTAATCTCAC+TGG 0.518273 4_1:+77773024 Msa0574320:five_prime_UTR
ACACTTTGCAACACTTCGAA+TGG 0.529196 4_1:-77774456 None:intergenic
TGCTAGAACATGCATTGCAT+GGG 0.532292 4_1:-77774668 None:intergenic
CTTTATGGTGCAGAGGCTGT+AGG 0.534556 4_1:+77774780 Msa0574320:three_prime_UTR
TTATTCTAGATAAATTCCAT+TGG 0.541838 4_1:+77773062 Msa0574320:five_prime_UTR
TTGCTAGAACATGCATTGCA+TGG 0.544380 4_1:-77774669 None:intergenic
AAACATATATCAAAGCTTGA+AGG 0.550802 4_1:+77774044 Msa0574320:CDS
TGCACAGCCATGACAATGCA+TGG 0.563796 4_1:-77774009 None:intergenic
AGTGTTGCAAAGTGTATCAA+AGG 0.566152 4_1:+77774464 Msa0574320:CDS
ATTGACCAAACTAAATCCAA+TGG 0.577521 4_1:-77772981 None:intergenic
TCTCTGTCACTTGCAATCAA+GGG 0.585982 4_1:-77773349 None:intergenic
TTGGTGGAATCTGCTAATGC+CGG 0.598406 4_1:-77774562 None:intergenic
TCTGCATTGCTAAACTCCAC+CGG 0.607232 4_1:+77774543 Msa0574320:three_prime_UTR
TTCCTTCAACTCACATAATG+GGG 0.610996 4_1:+77773227 Msa0574320:exon
GTGGAATCTGCTAATGCCGG+TGG 0.615330 4_1:-77774559 None:intergenic
ACATTTGCTTTATGGTGCAG+AGG 0.619699 4_1:+77774773 Msa0574320:three_prime_UTR
TGTGCATGATTCATCCGAGA+AGG 0.632281 4_1:-77774753 None:intergenic
TGTTGCTGGAAAGCAAACAT+TGG 0.652091 4_1:+77773399 Msa0574320:CDS
AAGGTATCCATGCATTGTCA+TGG 0.661899 4_1:+77774002 Msa0574320:CDS
TGGAGACAGATACCAAACAT+TGG 0.669694 4_1:-77772961 None:intergenic
GCTAGAACATGCATTGCATG+GGG 0.757483 4_1:-77774667 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTCATTATCAAAATTTA+TGG - chr4_1:77773320-77773339 None:intergenic 15.0%
!!! ATTTTAAAATATAAGCAATC+TGG - chr4_1:77773515-77773534 None:intergenic 15.0%
!! ACAAATACAAACAAAATTAA+AGG - chr4_1:77774085-77774104 None:intergenic 15.0%
!!! ATTTTTTCTTTATAATTAGC+AGG - chr4_1:77774517-77774536 None:intergenic 15.0%
!!! CATCTTTTAATTAGTAAATT+TGG + chr4_1:77774843-77774862 Msa0574320:three_prime_UTR 15.0%
!! AAACTCATTATCAAAATTTA+TGG - chr4_1:77773320-77773339 None:intergenic 15.0%
!!! ATTTTAAAATATAAGCAATC+TGG - chr4_1:77773515-77773534 None:intergenic 15.0%
!! ACAAATACAAACAAAATTAA+AGG - chr4_1:77774085-77774104 None:intergenic 15.0%
!!! ATTTTTTCTTTATAATTAGC+AGG - chr4_1:77774517-77774536 None:intergenic 15.0%
!! ATTAATAGAGAGATTAGAAA+AGG - chr4_1:77772874-77772893 None:intergenic 20.0%
!! AAACAGAAATGTATTAAAAG+AGG - chr4_1:77772920-77772939 None:intergenic 20.0%
!! TTATTCTAGATAAATTCCAT+TGG + chr4_1:77773062-77773081 Msa0574320:five_prime_UTR 20.0%
!!! TTAAGGTGAAAATTTTATTG+GGG - chr4_1:77773111-77773130 None:intergenic 20.0%
!!! CTTAAGGTGAAAATTTTATT+GGG - chr4_1:77773112-77773131 None:intergenic 20.0%
!!! TTAGAATATTGATAATGTAG+AGG + chr4_1:77773782-77773801 Msa0574320:intron 20.0%
!! GATAATATATCTCATAAGAT+GGG - chr4_1:77774210-77774229 None:intergenic 20.0%
!! AGATAATATATCTCATAAGA+TGG - chr4_1:77774211-77774230 None:intergenic 20.0%
!! TAAGATATAGTATCAAAATG+TGG + chr4_1:77774304-77774323 Msa0574320:intron 20.0%
!! AGAGAGAAAAAAAATAATGT+TGG + chr4_1:77774719-77774738 Msa0574320:three_prime_UTR 20.0%
!! ATTAATAGAGAGATTAGAAA+AGG - chr4_1:77772874-77772893 None:intergenic 20.0%
!! AAACAGAAATGTATTAAAAG+AGG - chr4_1:77772920-77772939 None:intergenic 20.0%
!! TTATTCTAGATAAATTCCAT+TGG + chr4_1:77773062-77773081 Msa0574320:five_prime_UTR 20.0%
!!! TTAAGGTGAAAATTTTATTG+GGG - chr4_1:77773111-77773130 None:intergenic 20.0%
!!! CTTAAGGTGAAAATTTTATT+GGG - chr4_1:77773112-77773131 None:intergenic 20.0%
!!! TTAGAATATTGATAATGTAG+AGG + chr4_1:77773782-77773801 Msa0574320:intron 20.0%
!! GATAATATATCTCATAAGAT+GGG - chr4_1:77774210-77774229 None:intergenic 20.0%
!! AGATAATATATCTCATAAGA+TGG - chr4_1:77774211-77774230 None:intergenic 20.0%
!! TAAGATATAGTATCAAAATG+TGG + chr4_1:77774304-77774323 Msa0574320:intron 20.0%
!! AGAGAGAAAAAAAATAATGT+TGG + chr4_1:77774719-77774738 Msa0574320:three_prime_UTR 20.0%
!! ATTGGTGACATAACTATAAA+TGG - chr4_1:77772946-77772965 None:intergenic 25.0%
! ATGACAAAATTTAATCTCAC+TGG + chr4_1:77773024-77773043 Msa0574320:five_prime_UTR 25.0%
!! GCTTAAGGTGAAAATTTTAT+TGG - chr4_1:77773113-77773132 None:intergenic 25.0%
!!! TTCCTTTTTTCCTTAACATA+AGG + chr4_1:77773158-77773177 Msa0574320:five_prime_UTR 25.0%
! TTCCTTATGTTAAGGAAAAA+AGG - chr4_1:77773163-77773182 None:intergenic 25.0%
! TTAAAATATAAGCAATCTGG+TGG - chr4_1:77773512-77773531 None:intergenic 25.0%
! ATTAACATTGTGCAACTATA+GGG - chr4_1:77773541-77773560 None:intergenic 25.0%
! TGAAATCAGATCAAGATAAA+GGG - chr4_1:77773573-77773592 None:intergenic 25.0%
! TTGAAATCAGATCAAGATAA+AGG - chr4_1:77773574-77773593 None:intergenic 25.0%
! AAGTTAAAAATGACAGAGAT+GGG - chr4_1:77773598-77773617 None:intergenic 25.0%
! TAAGTTAAAAATGACAGAGA+TGG - chr4_1:77773599-77773618 None:intergenic 25.0%
! GAACTATAATGACATCAAAT+CGG - chr4_1:77773646-77773665 None:intergenic 25.0%
! ATTCACATCTTACAATTTCA+AGG + chr4_1:77773754-77773773 Msa0574320:intron 25.0%
! TAAAATTTCAGAGATCAACT+TGG + chr4_1:77773832-77773851 Msa0574320:intron 25.0%
! TGGATATTTACTCTTCATTA+TGG + chr4_1:77773852-77773871 Msa0574320:intron 25.0%
! TATGCAGATAGTAATATTGA+AGG + chr4_1:77773983-77774002 Msa0574320:intron 25.0%
! AAACATATATCAAAGCTTGA+AGG + chr4_1:77774044-77774063 Msa0574320:CDS 25.0%
! GAAGATGAAACAAATTGTAT+TGG + chr4_1:77774149-77774168 Msa0574320:intron 25.0%
! TTAATGTGATAAAAGCAAAG+AGG + chr4_1:77774172-77774191 Msa0574320:intron 25.0%
! TTAGCCATCTTGATATATAA+AGG + chr4_1:77774230-77774249 Msa0574320:intron 25.0%
!! TGAATTTTTCATATGTGACT+TGG - chr4_1:77774256-77774275 None:intergenic 25.0%
!! TTTTCTTTATAATTAGCAGG+TGG - chr4_1:77774514-77774533 None:intergenic 25.0%
!! ATTGGTGACATAACTATAAA+TGG - chr4_1:77772946-77772965 None:intergenic 25.0%
! ATGACAAAATTTAATCTCAC+TGG + chr4_1:77773024-77773043 Msa0574320:five_prime_UTR 25.0%
!! GCTTAAGGTGAAAATTTTAT+TGG - chr4_1:77773113-77773132 None:intergenic 25.0%
!!! TTCCTTTTTTCCTTAACATA+AGG + chr4_1:77773158-77773177 Msa0574320:five_prime_UTR 25.0%
! TTCCTTATGTTAAGGAAAAA+AGG - chr4_1:77773163-77773182 None:intergenic 25.0%
! TTAAAATATAAGCAATCTGG+TGG - chr4_1:77773512-77773531 None:intergenic 25.0%
! ATTAACATTGTGCAACTATA+GGG - chr4_1:77773541-77773560 None:intergenic 25.0%
! TGAAATCAGATCAAGATAAA+GGG - chr4_1:77773573-77773592 None:intergenic 25.0%
! TTGAAATCAGATCAAGATAA+AGG - chr4_1:77773574-77773593 None:intergenic 25.0%
! AAGTTAAAAATGACAGAGAT+GGG - chr4_1:77773598-77773617 None:intergenic 25.0%
! TAAGTTAAAAATGACAGAGA+TGG - chr4_1:77773599-77773618 None:intergenic 25.0%
! GAACTATAATGACATCAAAT+CGG - chr4_1:77773646-77773665 None:intergenic 25.0%
! ATTCACATCTTACAATTTCA+AGG + chr4_1:77773754-77773773 Msa0574320:intron 25.0%
! TAAAATTTCAGAGATCAACT+TGG + chr4_1:77773832-77773851 Msa0574320:intron 25.0%
! TGGATATTTACTCTTCATTA+TGG + chr4_1:77773852-77773871 Msa0574320:intron 25.0%
! TATGCAGATAGTAATATTGA+AGG + chr4_1:77773983-77774002 Msa0574320:intron 25.0%
! AAACATATATCAAAGCTTGA+AGG + chr4_1:77774044-77774063 Msa0574320:CDS 25.0%
! GAAGATGAAACAAATTGTAT+TGG + chr4_1:77774149-77774168 Msa0574320:intron 25.0%
! TTAATGTGATAAAAGCAAAG+AGG + chr4_1:77774172-77774191 Msa0574320:intron 25.0%
! TTAGCCATCTTGATATATAA+AGG + chr4_1:77774230-77774249 Msa0574320:intron 25.0%
!! TGAATTTTTCATATGTGACT+TGG - chr4_1:77774256-77774275 None:intergenic 25.0%
!! TTTTCTTTATAATTAGCAGG+TGG - chr4_1:77774514-77774533 None:intergenic 25.0%
ATAGTTATGTCACCAATGTT+TGG + chr4_1:77772949-77772968 Msa0574320:five_prime_UTR 30.0%
ATTGACCAAACTAAATCCAA+TGG - chr4_1:77772984-77773003 None:intergenic 30.0%
GTTTGGTCAATGTAATTTAG+TGG + chr4_1:77772993-77773012 Msa0574320:five_prime_UTR 30.0%
TCAAATTGCACAAAAACCAA+TGG - chr4_1:77773081-77773100 None:intergenic 30.0%
AAAAAAGGAACATCAGGTAT+GGG - chr4_1:77773148-77773167 None:intergenic 30.0%
TTAAGGAAAAAAGGAACATC+AGG - chr4_1:77773154-77773173 None:intergenic 30.0%
!!! TGTGTTTTGAGAGCATATAT+AGG + chr4_1:77773197-77773216 Msa0574320:five_prime_UTR 30.0%
ATTCCTTCAACTCACATAAT+GGG + chr4_1:77773226-77773245 Msa0574320:exon 30.0%
!! GATACATTCTGTTTTTCTTC+TGG + chr4_1:77773268-77773287 Msa0574320:CDS 30.0%
TGATCAAAAACTACGATTGA+AGG + chr4_1:77773372-77773391 Msa0574320:CDS 30.0%
GATTAACATTGTGCAACTAT+AGG - chr4_1:77773542-77773561 None:intergenic 30.0%
AATATATCTCATAAGATGGG+AGG - chr4_1:77774207-77774226 None:intergenic 30.0%
! TTGGCCTTTATATATCAAGA+TGG - chr4_1:77774237-77774256 None:intergenic 30.0%
TATAGTATCAAAATGTGGCA+TGG + chr4_1:77774309-77774328 Msa0574320:intron 30.0%
! AGGTTAAAAATGCACAGTTT+TGG + chr4_1:77774484-77774503 Msa0574320:CDS 30.0%
AATCATGCACATTTGCTTTA+TGG + chr4_1:77774765-77774784 Msa0574320:three_prime_UTR 30.0%
ATAGTTATGTCACCAATGTT+TGG + chr4_1:77772949-77772968 Msa0574320:five_prime_UTR 30.0%
ATTGACCAAACTAAATCCAA+TGG - chr4_1:77772984-77773003 None:intergenic 30.0%
GTTTGGTCAATGTAATTTAG+TGG + chr4_1:77772993-77773012 Msa0574320:five_prime_UTR 30.0%
TCAAATTGCACAAAAACCAA+TGG - chr4_1:77773081-77773100 None:intergenic 30.0%
AAAAAAGGAACATCAGGTAT+GGG - chr4_1:77773148-77773167 None:intergenic 30.0%
TTAAGGAAAAAAGGAACATC+AGG - chr4_1:77773154-77773173 None:intergenic 30.0%
!!! TGTGTTTTGAGAGCATATAT+AGG + chr4_1:77773197-77773216 Msa0574320:five_prime_UTR 30.0%
ATTCCTTCAACTCACATAAT+GGG + chr4_1:77773226-77773245 Msa0574320:exon 30.0%
!! GATACATTCTGTTTTTCTTC+TGG + chr4_1:77773268-77773287 Msa0574320:CDS 30.0%
TGATCAAAAACTACGATTGA+AGG + chr4_1:77773372-77773391 Msa0574320:CDS 30.0%
GATTAACATTGTGCAACTAT+AGG - chr4_1:77773542-77773561 None:intergenic 30.0%
AATATATCTCATAAGATGGG+AGG - chr4_1:77774207-77774226 None:intergenic 30.0%
! TTGGCCTTTATATATCAAGA+TGG - chr4_1:77774237-77774256 None:intergenic 30.0%
TATAGTATCAAAATGTGGCA+TGG + chr4_1:77774309-77774328 Msa0574320:intron 30.0%
! AGGTTAAAAATGCACAGTTT+TGG + chr4_1:77774484-77774503 Msa0574320:CDS 30.0%
AATCATGCACATTTGCTTTA+TGG + chr4_1:77774765-77774784 Msa0574320:three_prime_UTR 30.0%
TGTCTCCATTGGATTTAGTT+TGG + chr4_1:77772976-77772995 Msa0574320:five_prime_UTR 35.0%
GGGAAAAAAGAAGAAGCTTA+AGG - chr4_1:77773128-77773147 None:intergenic 35.0%
GAAAAAAGGAACATCAGGTA+TGG - chr4_1:77773149-77773168 None:intergenic 35.0%
CACAACACTTCCTTATGTTA+AGG - chr4_1:77773171-77773190 None:intergenic 35.0%
GATTCCTTCAACTCACATAA+TGG + chr4_1:77773225-77773244 Msa0574320:exon 35.0%
TTCCTTCAACTCACATAATG+GGG + chr4_1:77773227-77773246 Msa0574320:exon 35.0%
! GGCTTTTCAATTGAAACCTA+AGG + chr4_1:77773420-77773439 Msa0574320:CDS 35.0%
GAGAAGAAAAACTTCACCTT+AGG - chr4_1:77773439-77773458 None:intergenic 35.0%
AGTGTTGCAAAGTGTATCAA+AGG + chr4_1:77774464-77774483 Msa0574320:CDS 35.0%
!! TATGTCTACTTTTGTCTTGG+TGG - chr4_1:77774581-77774600 None:intergenic 35.0%
!! GAGTATGTCTACTTTTGTCT+TGG - chr4_1:77774584-77774603 None:intergenic 35.0%
TGTCTCCATTGGATTTAGTT+TGG + chr4_1:77772976-77772995 Msa0574320:five_prime_UTR 35.0%
GGGAAAAAAGAAGAAGCTTA+AGG - chr4_1:77773128-77773147 None:intergenic 35.0%
GAAAAAAGGAACATCAGGTA+TGG - chr4_1:77773149-77773168 None:intergenic 35.0%
CACAACACTTCCTTATGTTA+AGG - chr4_1:77773171-77773190 None:intergenic 35.0%
GATTCCTTCAACTCACATAA+TGG + chr4_1:77773225-77773244 Msa0574320:exon 35.0%
TTCCTTCAACTCACATAATG+GGG + chr4_1:77773227-77773246 Msa0574320:exon 35.0%
! GGCTTTTCAATTGAAACCTA+AGG + chr4_1:77773420-77773439 Msa0574320:CDS 35.0%
GAGAAGAAAAACTTCACCTT+AGG - chr4_1:77773439-77773458 None:intergenic 35.0%
AGTGTTGCAAAGTGTATCAA+AGG + chr4_1:77774464-77774483 Msa0574320:CDS 35.0%
!! TATGTCTACTTTTGTCTTGG+TGG - chr4_1:77774581-77774600 None:intergenic 35.0%
!! GAGTATGTCTACTTTTGTCT+TGG - chr4_1:77774584-77774603 None:intergenic 35.0%
TGGAGACAGATACCAAACAT+TGG - chr4_1:77772964-77772983 None:intergenic 40.0%
TGTTTGGTATCTGTCTCCAT+TGG + chr4_1:77772965-77772984 Msa0574320:five_prime_UTR 40.0%
! TTCCCCATTATGTGAGTTGA+AGG - chr4_1:77773232-77773251 None:intergenic 40.0%
! ACTCACATAATGGGGAAACT+TGG + chr4_1:77773235-77773254 Msa0574320:exon 40.0%
GGGGAAACTTGGTAAAATGT+TGG + chr4_1:77773246-77773265 Msa0574320:CDS 40.0%
! TCTCTGTCACTTGCAATCAA+GGG - chr4_1:77773352-77773371 None:intergenic 40.0%
! ATCTCTGTCACTTGCAATCA+AGG - chr4_1:77773353-77773372 None:intergenic 40.0%
TGTTGCTGGAAAGCAAACAT+TGG + chr4_1:77773399-77773418 Msa0574320:CDS 40.0%
TTCCTGCAAATACCAACCAA+CGG + chr4_1:77773663-77773682 Msa0574320:intron 40.0%
! AAGGTATCCATGCATTGTCA+TGG + chr4_1:77774002-77774021 Msa0574320:CDS 40.0%
AATGTGGCATGGTATAGACA+TGG + chr4_1:77774320-77774339 Msa0574320:intron 40.0%
ATGTGGCATGGTATAGACAT+GGG + chr4_1:77774321-77774340 Msa0574320:intron 40.0%
AACAGGAGTGAGCTCATATA+AGG + chr4_1:77774389-77774408 Msa0574320:intron 40.0%
GGTAGATCTAGACACCAAAA+TGG + chr4_1:77774410-77774429 Msa0574320:CDS 40.0%
ACCAAAATGGTTGCTGTCAT+TGG + chr4_1:77774423-77774442 Msa0574320:CDS 40.0%
ACACTTTGCAACACTTCGAA+TGG - chr4_1:77774459-77774478 None:intergenic 40.0%
TGCTAGAACATGCATTGCAT+GGG - chr4_1:77774671-77774690 None:intergenic 40.0%
TTGCTAGAACATGCATTGCA+TGG - chr4_1:77774672-77774691 None:intergenic 40.0%
TGGTTGATCTTTCTCCTTCT+CGG + chr4_1:77774739-77774758 Msa0574320:three_prime_UTR 40.0%
ACATTTGCTTTATGGTGCAG+AGG + chr4_1:77774773-77774792 Msa0574320:three_prime_UTR 40.0%
TGGAGACAGATACCAAACAT+TGG - chr4_1:77772964-77772983 None:intergenic 40.0%
TGTTTGGTATCTGTCTCCAT+TGG + chr4_1:77772965-77772984 Msa0574320:five_prime_UTR 40.0%
! TTCCCCATTATGTGAGTTGA+AGG - chr4_1:77773232-77773251 None:intergenic 40.0%
! ACTCACATAATGGGGAAACT+TGG + chr4_1:77773235-77773254 Msa0574320:exon 40.0%
GGGGAAACTTGGTAAAATGT+TGG + chr4_1:77773246-77773265 Msa0574320:CDS 40.0%
! TCTCTGTCACTTGCAATCAA+GGG - chr4_1:77773352-77773371 None:intergenic 40.0%
! ATCTCTGTCACTTGCAATCA+AGG - chr4_1:77773353-77773372 None:intergenic 40.0%
TGTTGCTGGAAAGCAAACAT+TGG + chr4_1:77773399-77773418 Msa0574320:CDS 40.0%
TTCCTGCAAATACCAACCAA+CGG + chr4_1:77773663-77773682 Msa0574320:intron 40.0%
! AAGGTATCCATGCATTGTCA+TGG + chr4_1:77774002-77774021 Msa0574320:CDS 40.0%
AATGTGGCATGGTATAGACA+TGG + chr4_1:77774320-77774339 Msa0574320:intron 40.0%
ATGTGGCATGGTATAGACAT+GGG + chr4_1:77774321-77774340 Msa0574320:intron 40.0%
AACAGGAGTGAGCTCATATA+AGG + chr4_1:77774389-77774408 Msa0574320:intron 40.0%
GGTAGATCTAGACACCAAAA+TGG + chr4_1:77774410-77774429 Msa0574320:CDS 40.0%
ACCAAAATGGTTGCTGTCAT+TGG + chr4_1:77774423-77774442 Msa0574320:CDS 40.0%
ACACTTTGCAACACTTCGAA+TGG - chr4_1:77774459-77774478 None:intergenic 40.0%
TGCTAGAACATGCATTGCAT+GGG - chr4_1:77774671-77774690 None:intergenic 40.0%
TTGCTAGAACATGCATTGCA+TGG - chr4_1:77774672-77774691 None:intergenic 40.0%
TGGTTGATCTTTCTCCTTCT+CGG + chr4_1:77774739-77774758 Msa0574320:three_prime_UTR 40.0%
ACATTTGCTTTATGGTGCAG+AGG + chr4_1:77774773-77774792 Msa0574320:three_prime_UTR 40.0%
CGATTGAAGGATGTTGTTGC+TGG + chr4_1:77773385-77773404 Msa0574320:CDS 45.0%
! TTCCGTTGGTTGGTATTTGC+AGG - chr4_1:77773668-77773687 None:intergenic 45.0%
TGCAAATACCAACCAACGGA+AGG + chr4_1:77773667-77773686 Msa0574320:intron 45.0%
! TCTCATAAGATGGGAGGTGT+GGG - chr4_1:77774201-77774220 None:intergenic 45.0%
ATCTCATAAGATGGGAGGTG+TGG - chr4_1:77774202-77774221 None:intergenic 45.0%
TGTGGCATGGTATAGACATG+GGG + chr4_1:77774322-77774341 Msa0574320:intron 45.0%
GACTCTTTGCACTTACGAAC+AGG + chr4_1:77774372-77774391 Msa0574320:intron 45.0%
! GCCAATGACAGCAACCATTT+TGG - chr4_1:77774427-77774446 None:intergenic 45.0%
TCTGCATTGCTAAACTCCAC+CGG + chr4_1:77774543-77774562 Msa0574320:three_prime_UTR 45.0%
TTGGTGGAATCTGCTAATGC+CGG - chr4_1:77774565-77774584 None:intergenic 45.0%
GCTAGAACATGCATTGCATG+GGG - chr4_1:77774670-77774689 None:intergenic 45.0%
GTTCTAGCAACTCTCTTTGC+AGG + chr4_1:77774682-77774701 Msa0574320:three_prime_UTR 45.0%
TGTGCATGATTCATCCGAGA+AGG - chr4_1:77774756-77774775 None:intergenic 45.0%
CGATTGAAGGATGTTGTTGC+TGG + chr4_1:77773385-77773404 Msa0574320:CDS 45.0%
! TTCCGTTGGTTGGTATTTGC+AGG - chr4_1:77773668-77773687 None:intergenic 45.0%
TGCAAATACCAACCAACGGA+AGG + chr4_1:77773667-77773686 Msa0574320:intron 45.0%
! TCTCATAAGATGGGAGGTGT+GGG - chr4_1:77774201-77774220 None:intergenic 45.0%
ATCTCATAAGATGGGAGGTG+TGG - chr4_1:77774202-77774221 None:intergenic 45.0%
TGTGGCATGGTATAGACATG+GGG + chr4_1:77774322-77774341 Msa0574320:intron 45.0%
GACTCTTTGCACTTACGAAC+AGG + chr4_1:77774372-77774391 Msa0574320:intron 45.0%
! GCCAATGACAGCAACCATTT+TGG - chr4_1:77774427-77774446 None:intergenic 45.0%
TCTGCATTGCTAAACTCCAC+CGG + chr4_1:77774543-77774562 Msa0574320:three_prime_UTR 45.0%
TTGGTGGAATCTGCTAATGC+CGG - chr4_1:77774565-77774584 None:intergenic 45.0%
GCTAGAACATGCATTGCATG+GGG - chr4_1:77774670-77774689 None:intergenic 45.0%
GTTCTAGCAACTCTCTTTGC+AGG + chr4_1:77774682-77774701 Msa0574320:three_prime_UTR 45.0%
TGTGCATGATTCATCCGAGA+AGG - chr4_1:77774756-77774775 None:intergenic 45.0%
ATACCAACCAACGGAAGGAC+TGG + chr4_1:77773672-77773691 Msa0574320:intron 50.0%
TGCACAGCCATGACAATGCA+TGG - chr4_1:77774012-77774031 None:intergenic 50.0%
CTTTATGGTGCAGAGGCTGT+AGG + chr4_1:77774780-77774799 Msa0574320:three_prime_UTR 50.0%
ATACCAACCAACGGAAGGAC+TGG + chr4_1:77773672-77773691 Msa0574320:intron 50.0%
TGCACAGCCATGACAATGCA+TGG - chr4_1:77774012-77774031 None:intergenic 50.0%
CTTTATGGTGCAGAGGCTGT+AGG + chr4_1:77774780-77774799 Msa0574320:three_prime_UTR 50.0%
AGTTCGTCCAGTCCTTCCGT+TGG - chr4_1:77773682-77773701 None:intergenic 55.0%
GTGGAATCTGCTAATGCCGG+TGG - chr4_1:77774562-77774581 None:intergenic 55.0%
AGTTCGTCCAGTCCTTCCGT+TGG - chr4_1:77773682-77773701 None:intergenic 55.0%
GTGGAATCTGCTAATGCCGG+TGG - chr4_1:77774562-77774581 None:intergenic 55.0%
CGTCCAGTCCTTCCGTTGGT+TGG - chr4_1:77773678-77773697 None:intergenic 60.0%
CGTCCAGTCCTTCCGTTGGT+TGG - chr4_1:77773678-77773697 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 77772868 77774865 77772868 ID=Msa0574320;Name=Msa0574320
chr4_1 mRNA 77772868 77774865 77772868 ID=Msa0574320-mRNA-1;Parent=Msa0574320;Name=Msa0574320-mRNA-1;_AED=0.01;_eAED=0.01;_QI=376|1|1|1|1|1|3|344|133
chr4_1 exon 77772868 77773441 77772868 ID=Msa0574320-mRNA-1:exon:27404;Parent=Msa0574320-mRNA-1
chr4_1 exon 77773990 77774065 77773990 ID=Msa0574320-mRNA-1:exon:27405;Parent=Msa0574320-mRNA-1
chr4_1 exon 77774394 77774865 77774394 ID=Msa0574320-mRNA-1:exon:27406;Parent=Msa0574320-mRNA-1
chr4_1 five_prime_UTR 77772868 77773243 77772868 ID=Msa0574320-mRNA-1:five_prime_utr;Parent=Msa0574320-mRNA-1
chr4_1 CDS 77773244 77773441 77773244 ID=Msa0574320-mRNA-1:cds;Parent=Msa0574320-mRNA-1
chr4_1 CDS 77773990 77774065 77773990 ID=Msa0574320-mRNA-1:cds;Parent=Msa0574320-mRNA-1
chr4_1 CDS 77774394 77774521 77774394 ID=Msa0574320-mRNA-1:cds;Parent=Msa0574320-mRNA-1
chr4_1 three_prime_UTR 77774522 77774865 77774522 ID=Msa0574320-mRNA-1:three_prime_utr;Parent=Msa0574320-mRNA-1
Gene Sequence

>Msa0574320

ATGGGGAAACTTGGTAAAATGTTGGATACATTCTGTTTTTCTTCTGGTTCAAACTCATGCTTCTGTCTAAATTCCATAAATTTTGATAATGAGTTTGAGAGCAAGCCCTTGATTGCAAGTGACAGAGATGATCAAAAACTACGATTGAAGGATGTTGTTGCTGGAAAGCAAACATTGGCTTTTCAATTGAAACCTAAGATAGTAATATTGAAGGTATCCATGCATTGTCATGGCTGTGCAAGAAAAGTTGAGAAACATATATCAAAGCTTGAAGGAGTGAGCTCATATAAGGTAGATCTAGACACCAAAATGGTTGCTGTCATTGGCGATATTCTTCCATTCGAAGTGTTGCAAAGTGTATCAAAGGTTAAAAATGCACAGTTTTGGAATTCCACCTGCTAA
Protein sequence

>Msa0574320

MGKLGKMLDTFCFSSGSNSCFCLNSINFDNEFESKPLIASDRDDQKLRLKDVVAGKQTLAFQLKPKIVILKVSMHCHGCARKVEKHISKLEGVSSYKVDLDTKMVAVIGDILPFEVLQSVSKVKNAQFWNSTC*