AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0580490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0580490 MtrunA17_Chr5g0399221 97.527 364 6 1 1 361 1 364 0.0 743
Msa0580490 MtrunA17_Chr6g0487101 52.941 289 135 1 68 356 93 380 7.49e-108 321
Msa0580490 MtrunA17_Chr8g0389771 53.265 291 133 2 68 356 88 377 6.24e-106 316
Msa0580490 MtrunA17_Chr7g0258341 47.541 305 145 4 60 356 90 387 1.23e-92 282
Msa0580490 MtrunA17_Chr5g0446301 46.259 294 152 4 66 356 67 357 7.38e-92 279
Msa0580490 MtrunA17_Chr7g0265201 44.282 341 164 8 25 356 62 385 2.90e-87 268
Msa0580490 MtrunA17_Chr1g0194311 39.612 361 200 6 12 356 10 368 3.38e-84 260
Msa0580490 MtrunA17_Chr4g0070051 43.182 308 154 7 58 358 52 345 5.33e-76 238
Msa0580490 MtrunA17_Chr2g0294321 40.984 305 164 4 57 356 53 346 7.28e-71 225
Msa0580490 MtrunA17_Chr7g0217941 40.000 310 166 7 59 357 5 305 4.11e-70 222
Msa0580490 MtrunA17_Chr6g0466961 37.061 313 178 6 50 356 25 324 2.61e-68 218
Msa0580490 MtrunA17_Chr6g0453551 38.511 309 172 6 59 357 5 305 1.55e-67 215
Msa0580490 MtrunA17_Chr6g0466951 38.019 313 175 6 50 356 54 353 2.87e-66 213
Msa0580490 MtrunA17_Chr4g0037751 34.174 357 217 6 12 358 15 363 1.24e-65 212
Msa0580490 MtrunA17_Chr5g0420831 35.028 354 214 5 13 358 18 363 1.38e-65 212
Msa0580490 MtrunA17_Chr1g0161731 35.920 348 200 7 16 356 8 339 4.26e-64 207
Msa0580490 MtrunA17_Chr2g0295851 39.344 305 160 5 57 356 52 336 6.91e-64 206
Msa0580490 MtrunA17_Chr8g0351871 36.688 308 172 6 58 357 31 323 2.29e-62 202
Msa0580490 MtrunA17_Chr5g0440871 35.390 308 173 6 56 357 32 319 1.12e-52 177
Msa0580490 MtrunA17_Chr7g0272141 30.769 364 222 7 14 359 183 534 1.54e-47 169
Msa0580490 MtrunA17_Chr6g0459781 45.402 174 92 2 59 232 12 182 3.58e-47 159
Msa0580490 MtrunA17_Chr4g0069881 31.447 318 192 6 57 359 221 527 8.61e-47 166
Msa0580490 MtrunA17_Chr8g0345281 32.063 315 187 6 60 359 279 581 5.37e-46 165
Msa0580490 MtrunA17_Chr8g0384121 32.476 311 177 7 68 356 254 553 6.37e-46 165
Msa0580490 MtrunA17_Chr1g0192331 31.366 322 184 9 60 359 214 520 2.28e-44 160
Msa0580490 MtrunA17_Chr2g0314691 39.000 200 119 3 161 358 5 203 9.99e-43 147
Msa0580490 MtrunA17_Chr1g0195301 32.484 314 179 8 65 359 248 547 1.02e-42 155
Msa0580490 MtrunA17_Chr1g0195321 32.484 314 179 8 65 359 248 547 1.02e-42 155
Msa0580490 MtrunA17_Chr4g0069841 30.032 313 192 6 62 359 208 508 1.32e-42 155
Msa0580490 MtrunA17_Chr1g0175661 31.132 318 190 7 59 359 245 550 1.81e-42 155
Msa0580490 MtrunA17_Chr4g0011771 32.099 324 190 8 50 359 198 505 2.15e-42 154
Msa0580490 MtrunA17_Chr4g0025841 36.400 250 137 4 58 304 35 265 2.49e-42 148
Msa0580490 MtrunA17_Chr4g0018371 32.595 316 186 9 60 359 227 531 5.79e-42 153
Msa0580490 MtrunA17_Chr7g0269531 31.546 317 185 8 59 356 251 554 7.90e-42 154
Msa0580490 MtrunA17_Chr7g0265161 36.486 222 115 5 140 357 14 213 9.65e-42 146
Msa0580490 MtrunA17_Chr1g0195331 32.166 314 180 8 65 359 248 547 1.55e-41 152
Msa0580490 MtrunA17_Chr3g0090091 30.380 316 193 8 60 359 216 520 2.99e-41 151
Msa0580490 MtrunA17_Chr3g0080391 30.449 312 195 6 60 361 269 568 1.04e-40 150
Msa0580490 MtrunA17_Chr3g0079341 31.469 286 172 7 60 332 215 489 1.11e-40 150
Msa0580490 MtrunA17_Chr3g0079351 29.747 316 195 8 60 359 215 519 1.20e-40 150
Msa0580490 MtrunA17_Chr3g0079361 30.063 316 194 8 60 359 215 519 1.28e-40 149
Msa0580490 MtrunA17_Chr7g0269511 29.787 329 201 8 49 359 240 556 2.14e-40 149
Msa0580490 MtrunA17_Chr6g0460141 50.000 132 63 1 99 230 16 144 3.62e-40 139
Msa0580490 MtrunA17_Chr8g0354901 30.128 312 196 6 60 361 276 575 3.98e-40 149
Msa0580490 MtrunA17_Chr7g0269541 28.358 335 202 7 51 359 226 548 5.28e-40 148
Msa0580490 MtrunA17_Chr2g0302021 29.560 318 197 6 57 359 212 517 5.68e-40 148
Msa0580490 MtrunA17_Chr7g0269521 29.375 320 197 7 57 359 247 554 5.94e-40 148
Msa0580490 MtrunA17_Chr3g0129921 30.794 315 182 7 66 356 251 553 9.63e-40 148
Msa0580490 MtrunA17_Chr7g0233801 29.524 315 196 6 60 359 242 545 1.69e-39 147
Msa0580490 MtrunA17_Chr1g0195351 31.847 314 179 9 65 359 248 545 2.30e-39 146
Msa0580490 MtrunA17_Chr7g0233831 29.430 316 196 6 60 359 243 547 5.81e-39 145
Msa0580490 MtrunA17_Chr1g0147051 29.290 338 199 8 50 359 274 599 6.62e-39 146
Msa0580490 MtrunA17_Chr5g0437831 30.625 320 188 9 48 348 65 369 7.49e-38 139
Msa0580490 MtrunA17_Chr1g0192311 29.179 329 181 7 60 359 257 562 9.66e-38 142
Msa0580490 MtrunA17_Chr1g0192281 29.179 329 181 7 60 359 257 562 9.66e-38 142
Msa0580490 MtrunA17_Chr3g0080381 30.218 321 195 7 57 360 313 621 1.08e-37 143
Msa0580490 MtrunA17_Chr1g0192321 29.524 315 196 6 60 359 272 575 1.09e-37 142
Msa0580490 MtrunA17_Chr7g0233771 30.420 286 175 5 60 332 242 516 1.29e-37 142
Msa0580490 MtrunA17_Chr2g0295531 31.690 284 171 5 60 332 232 503 2.02e-37 142
Msa0580490 MtrunA17_Chr4g0069861 30.094 319 194 7 53 356 202 506 2.30e-37 140
Msa0580490 MtrunA17_Chr8g0354911 30.503 318 192 7 60 360 300 605 2.65e-37 141
Msa0580490 MtrunA17_Chr3g0126771 30.063 316 193 7 60 359 286 589 3.93e-37 141
Msa0580490 MtrunA17_Chr8g0391291 30.745 322 191 8 60 361 228 537 4.38e-37 140
Msa0580490 MtrunA17_Chr1g0192301 29.217 332 177 8 60 359 262 567 5.28e-37 140
Msa0580490 MtrunA17_Chr2g0302001 32.906 234 137 5 140 359 202 429 1.55e-36 137
Msa0580490 MtrunA17_Chr4g0000121 28.571 329 183 7 60 359 244 549 3.21e-36 138
Msa0580490 MtrunA17_Chr8g0391311 30.745 322 191 8 60 361 230 539 3.90e-36 137
Msa0580490 MtrunA17_Chr7g0270691 29.102 323 201 6 54 359 230 541 4.33e-36 137
Msa0580490 MtrunA17_Chr4g0011751 29.022 317 195 8 61 359 177 481 1.95e-35 135
Msa0580490 MtrunA17_Chr4g0011791 30.128 312 191 8 60 359 205 501 3.72e-35 134
Msa0580490 MtrunA17_Chr1g0195311 32.342 269 152 7 107 359 52 306 7.30e-35 130
Msa0580490 MtrunA17_Chr2g0334111 28.889 315 189 7 57 359 24 315 1.62e-34 129
Msa0580490 MtrunA17_Chr1g0194091 29.642 307 184 7 54 348 249 535 1.66e-34 133
Msa0580490 MtrunA17_Chr7g0268241 30.421 309 175 8 68 360 59 343 1.77e-34 129
Msa0580490 MtrunA17_Chr5g0440901 46.324 136 70 1 176 308 13 148 1.78e-34 124
Msa0580490 MtrunA17_Chr4g0069891 31.058 293 176 6 52 332 279 557 6.38e-34 132
Msa0580490 MtrunA17_Chr4g0067611 29.225 284 178 5 60 332 258 529 8.64e-34 131
Msa0580490 MtrunA17_Chr7g0234711 32.287 223 131 5 152 359 44 261 5.20e-33 124
Msa0580490 MtrunA17_Chr7g0272151 27.812 320 196 7 60 359 271 575 6.85e-33 129
Msa0580490 MtrunA17_Chr4g0067601 29.617 287 177 6 57 332 257 529 2.20e-32 127
Msa0580490 MtrunA17_Chr2g0301931 28.873 284 174 6 85 352 12 283 4.66e-32 122
Msa0580490 MtrunA17_Chr2g0295551 30.824 279 170 5 65 332 255 521 5.43e-32 126
Msa0580490 MtrunA17_Chr1g0194081 28.797 316 198 7 57 359 254 555 5.92e-31 123
Msa0580490 MtrunA17_Chr8g0345211 27.532 316 204 6 53 356 269 571 6.95e-31 123
Msa0580490 MtrunA17_Chr7g0270631 30.345 290 166 7 65 332 800 1075 4.81e-30 121
Msa0580490 MtrunA17_Chr7g0270631 29.286 280 174 5 65 332 252 519 2.29e-28 116
Msa0580490 MtrunA17_Chr8g0380581 25.737 373 234 11 15 359 11 368 1.04e-29 117
Msa0580490 MtrunA17_Chr7g0270651 29.861 288 176 7 57 332 258 531 1.15e-29 119
Msa0580490 MtrunA17_Chr7g0270661 29.966 297 181 8 49 332 249 531 1.19e-29 119
Msa0580490 MtrunA17_Chr7g0270671 29.966 297 181 8 49 332 249 531 1.19e-29 119
Msa0580490 MtrunA17_Chr2g0295541 27.900 319 174 6 56 332 248 552 1.10e-27 114
Msa0580490 MtrunA17_Chr5g0440891 38.764 178 88 3 56 230 41 200 1.54e-26 105
Msa0580490 MtrunA17_Chr7g0271381 26.606 327 195 10 60 359 240 548 7.38e-26 108
Msa0580490 MtrunA17_Chr6g0459791 36.752 117 74 0 241 357 1 117 5.46e-21 87.4
Msa0580490 MtrunA17_Chr4g0073911 32.620 187 108 4 71 250 19 194 2.78e-20 89.0
Msa0580490 MtrunA17_Chr5g0440881 30.303 132 88 2 228 356 9 139 2.36e-16 75.5
Msa0580490 MtrunA17_Chr2g0299431 26.293 232 139 6 51 265 34 250 1.06e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0580490 AT5G47500.1 70.914 361 84 2 9 361 15 362 0.0 545
Msa0580490 AT5G19730.1 51.903 289 138 1 68 356 94 381 9.29e-105 313
Msa0580490 AT2G36710.1 48.208 307 147 3 59 360 90 389 6.78e-96 291
Msa0580490 AT5G55590.1 49.502 301 148 3 58 356 80 378 9.29e-95 287
Msa0580490 AT2G36700.1 45.000 300 156 4 59 356 41 333 7.83e-87 266
Msa0580490 AT1G05310.1 44.337 309 157 4 60 360 91 392 8.54e-87 268
Msa0580490 AT5G55590.2 52.610 249 115 2 58 304 80 327 1.07e-85 263
Msa0580490 AT5G07430.1 42.384 302 164 4 58 358 65 357 5.37e-85 262
Msa0580490 AT2G21610.1 43.046 302 153 6 59 358 50 334 1.65e-81 253
Msa0580490 AT2G21610.2 43.046 302 153 6 59 358 55 339 2.46e-81 252
Msa0580490 AT1G69940.1 41.311 305 169 4 58 361 65 360 1.23e-79 248
Msa0580490 AT5G07420.1 41.967 305 167 4 58 361 65 360 2.15e-79 248
Msa0580490 AT5G07410.1 40.984 305 170 4 58 361 65 360 8.57e-79 246
Msa0580490 AT5G61680.1 40.728 302 170 3 58 358 67 360 6.71e-77 241
Msa0580490 AT3G17060.1 36.158 354 201 6 8 356 3 336 9.15e-74 233
Msa0580490 AT3G29090.1 40.514 311 165 7 58 357 5 306 4.61e-70 222
Msa0580490 AT5G26810.1 32.603 365 198 8 10 357 1 334 4.83e-60 197
Msa0580490 AT3G24130.1 34.967 306 179 4 60 356 34 328 9.06e-60 196
Msa0580490 AT2G47280.1 38.111 307 168 6 60 357 35 328 1.81e-59 195
Msa0580490 AT5G18990.1 33.987 306 182 4 60 356 29 323 3.15e-57 189
Msa0580490 AT3G05620.1 35.974 303 177 6 62 359 243 533 1.13e-52 183
Msa0580490 AT2G19150.1 35.621 306 180 4 58 357 39 333 1.34e-50 172
Msa0580490 AT5G09760.1 34.277 318 175 7 60 355 241 546 2.78e-49 174
Msa0580490 AT5G09760.2 34.277 318 175 7 60 355 267 572 3.50e-49 174
Msa0580490 AT3G05610.1 33.441 311 181 6 60 356 256 554 2.19e-48 173
Msa0580490 AT3G06830.1 29.211 380 228 10 8 359 194 560 7.68e-48 170
Msa0580490 AT2G19150.2 39.044 251 137 3 58 302 39 279 2.19e-45 157
Msa0580490 AT5G27870.1 31.833 311 186 6 60 356 253 551 2.26e-45 166
Msa0580490 AT5G49180.1 32.698 315 183 7 60 358 260 561 6.95e-45 162
Msa0580490 AT5G64640.1 31.790 324 186 8 60 359 289 601 7.83e-45 162
Msa0580490 AT4G02330.1 31.429 315 187 7 59 356 258 560 7.07e-44 159
Msa0580490 AT5G20860.2 33.123 317 179 10 60 359 230 530 1.62e-43 158
Msa0580490 AT2G26440.1 30.530 321 195 7 53 359 233 539 2.51e-43 157
Msa0580490 AT3G59010.1 33.007 306 181 8 62 359 231 520 3.54e-43 157
Msa0580490 AT3G43270.1 31.138 334 193 7 51 359 196 517 1.65e-41 152
Msa0580490 AT1G11590.1 30.573 314 191 7 60 359 216 516 3.64e-41 151
Msa0580490 AT3G27980.1 30.573 314 191 7 60 359 189 489 4.93e-41 150
Msa0580490 AT4G33220.2 29.129 333 200 7 51 359 74 394 6.37e-41 148
Msa0580490 AT4G02300.1 31.761 318 190 7 57 359 219 524 1.02e-40 150
Msa0580490 AT3G10720.2 30.519 308 185 7 70 360 313 608 1.16e-40 151
Msa0580490 AT1G11580.1 29.206 315 196 6 60 359 247 549 2.46e-40 150
Msa0580490 AT1G02810.1 31.834 289 170 6 59 332 264 540 2.46e-40 150
Msa0580490 AT1G11580.2 29.206 315 196 6 60 359 268 570 2.67e-40 150
Msa0580490 AT1G23200.1 31.715 309 189 8 60 359 251 546 3.38e-40 149
Msa0580490 AT4G33220.1 29.129 333 200 7 51 359 195 515 3.47e-40 149
Msa0580490 AT3G14310.1 32.698 315 183 7 61 359 283 584 4.11e-40 149
Msa0580490 AT4G00190.1 30.938 320 194 9 55 359 159 466 6.09e-40 147
Msa0580490 AT1G53840.1 29.355 310 198 5 60 359 281 579 1.55e-39 148
Msa0580490 AT3G10710.1 30.000 310 195 6 60 359 256 553 2.59e-39 147
Msa0580490 AT1G53830.1 31.847 314 187 7 61 359 278 579 6.22e-39 146
Msa0580490 AT5G04970.1 31.068 309 182 8 70 360 318 613 9.08e-39 146
Msa0580490 AT4G15980.1 33.121 314 185 8 60 359 392 694 1.14e-38 146
Msa0580490 AT4G15980.2 33.121 314 185 8 60 359 402 704 1.41e-38 146
Msa0580490 AT1G11370.1 29.323 266 163 5 46 299 34 286 3.40e-38 138
Msa0580490 AT2G47550.1 31.447 318 189 8 59 359 245 550 7.94e-38 143
Msa0580490 AT4G33230.1 28.857 350 217 9 26 356 259 595 4.11e-37 141
Msa0580490 AT5G04960.1 28.617 311 199 6 60 359 258 556 7.17e-37 140
Msa0580490 AT4G03930.1 28.981 314 196 7 60 359 216 516 8.54e-37 139
Msa0580490 AT5G51490.1 28.571 378 235 10 3 359 165 528 2.92e-36 138
Msa0580490 AT2G45220.1 30.599 317 188 10 54 356 203 501 3.59e-36 137
Msa0580490 AT5G53370.1 30.063 316 193 7 60 359 274 577 4.86e-36 138
Msa0580490 AT5G53370.2 30.063 316 193 7 60 359 310 613 4.88e-36 138
Msa0580490 AT2G47030.1 32.867 286 163 6 65 334 282 554 4.90e-36 138
Msa0580490 AT2G26450.1 27.476 313 201 6 61 359 303 603 1.16e-35 137
Msa0580490 AT3G49220.3 31.329 316 189 8 60 359 330 633 2.01e-35 137
Msa0580490 AT3G49220.1 31.329 316 189 8 60 359 285 588 2.29e-35 136
Msa0580490 AT2G43050.1 30.072 276 175 5 90 359 245 508 2.84e-35 135
Msa0580490 AT4G02320.1 27.928 333 211 7 44 359 190 510 4.11e-35 135
Msa0580490 AT3G14300.1 28.994 338 205 10 41 356 634 958 4.93e-35 136
Msa0580490 AT3G10720.1 32.340 235 139 5 140 360 24 252 1.20e-34 128
Msa0580490 AT3G47400.1 30.133 375 215 12 14 359 230 586 1.21e-34 134
Msa0580490 AT2G47040.1 32.155 283 169 8 65 334 289 561 2.60e-34 133
Msa0580490 AT3G60730.1 31.104 299 179 8 76 359 225 511 6.61e-34 131
Msa0580490 AT5G20860.1 33.333 249 137 8 113 344 251 487 2.97e-32 127
Msa0580490 AT3G62170.1 31.119 286 168 7 65 334 282 554 1.77e-31 125
Msa0580490 AT5G51500.1 27.979 386 223 13 3 356 167 529 1.44e-30 122
Msa0580490 AT1G44980.1 24.405 168 114 3 68 234 86 241 7.28e-11 62.4

Find 126 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCGTGGCGTTTCGCATTTC+AGG 0.128654 4_1:+85590133 Msa0580490:CDS
ACATTCATTGTATTGTTATC+TGG 0.170780 4_1:-85588935 None:intergenic
ACATTCTTTGCAATTGCTTT+TGG 0.241254 4_1:+85588715 Msa0580490:CDS
ACGAAGCTCTTCCTGATAAA+TGG 0.247766 4_1:-85591632 None:intergenic
TACAATGTGTGTAAGTAAAA+AGG 0.263182 4_1:-85588530 None:intergenic
CACACGGTGGTTGGGATGAT+TGG 0.283922 4_1:+85591385 Msa0580490:CDS
TTAATTAGGACTGTGTTCTT+TGG 0.291942 4_1:+85591527 Msa0580490:intron
TGTATTTATACTATATATCT+TGG 0.300937 4_1:-85588596 None:intergenic
GTGAAGCCTGTCTTCTCGTC+TGG 0.316654 4_1:-85591255 None:intergenic
AAACAGCACATCCATTTATC+AGG 0.324261 4_1:+85591621 Msa0580490:CDS
GTTATCTGGGATGGAATTTA+CGG 0.330420 4_1:-85588921 None:intergenic
ACAAATAAGAATAGAATGTT+TGG 0.332719 4_1:-85588646 None:intergenic
CAAGAATAATGCTATATATA+TGG 0.342595 4_1:-85591692 None:intergenic
CAATTCTCAACTCTCTATAA+TGG 0.365946 4_1:+85588676 Msa0580490:exon
CATTCATTGTATTGTTATCT+GGG 0.374852 4_1:-85588934 None:intergenic
AGGTTCGATCCATTGGTTCT+TGG 0.385472 4_1:-85588834 None:intergenic
CCGTGGCGTTTCGCATTTCA+GGG 0.392449 4_1:+85590134 Msa0580490:CDS
TATACTAATATTTACCTTGA+AGG 0.396670 4_1:-85589381 None:intergenic
TAATAAAGAAATGCTTATAA+AGG 0.398499 4_1:+85591913 Msa0580490:three_prime_UTR
AGGCAGTACGTGGGGTGTCA+TGG 0.398827 4_1:+85591579 Msa0580490:CDS
CATTTCTTTATTACAATTGA+AGG 0.404631 4_1:-85591903 None:intergenic
TGCAAAGAATATCACCTTCA+AGG 0.406243 4_1:+85589367 Msa0580490:CDS
CGTCTTGGACGGACTGGAAC+TGG 0.408345 4_1:-85588898 None:intergenic
CGATGCAAGGTGACAGGCTC+AGG 0.411228 4_1:+85591285 Msa0580490:CDS
CTTTGGAGTGTACAAATGTT+GGG 0.416963 4_1:+85591544 Msa0580490:CDS
TGTAGTAGACAATGTATTGA+AGG 0.418098 4_1:-85588804 None:intergenic
TGCATCCCGGGCATTGGTGC+CGG 0.422340 4_1:-85590100 None:intergenic
CTATATGTAGGACGTGCAAT+GGG 0.427320 4_1:+85591312 Msa0580490:CDS
CCACGTACTGCCTCTGCTCC+TGG 0.427663 4_1:-85591569 None:intergenic
GGATGGAATTTACGGCGTCT+TGG 0.434709 4_1:-85588913 None:intergenic
TTGCCTATCCTGTGCTGCTA+TGG 0.436377 4_1:-85591229 None:intergenic
GTATTTATACTATATATCTT+GGG 0.437627 4_1:-85588595 None:intergenic
ACAGCGCCGGCACCAATGCC+CGG 0.437990 4_1:+85590094 Msa0580490:CDS
TACCGACAGGTTCGATCCAT+TGG 0.440708 4_1:-85588841 None:intergenic
CATGGTGATTAAAATATTTG+TGG 0.443377 4_1:-85588570 None:intergenic
ATTCTTTGCAGAGAAATAGT+TGG 0.443410 4_1:-85589354 None:intergenic
ATTGCACGTCCTACATATAG+TGG 0.444361 4_1:-85591309 None:intergenic
AATCCTTGCATCCCGGGCAT+TGG 0.445967 4_1:-85590106 None:intergenic
CATTGTATTGTTATCTGGGA+TGG 0.447321 4_1:-85588930 None:intergenic
GAGAAATAACAGTGATTGAA+TGG 0.452312 4_1:+85589255 Msa0580490:CDS
TCAGGAAGAGCTTCGTCAAT+GGG 0.453223 4_1:+85591639 Msa0580490:CDS
TCCCCTTGAAATGTTATGTA+CGG 0.454195 4_1:-85589227 None:intergenic
TGCTGGTAGGCATTACTTCA+AGG 0.455937 4_1:+85590216 Msa0580490:CDS
ACACGGTGGTTGGGATGATT+GGG 0.462473 4_1:+85591386 Msa0580490:CDS
GGAATTTACGGCGTCTTGGA+CGG 0.462494 4_1:-85588909 None:intergenic
TTCTCCGGCTGTGGCTTCCA+TGG 0.464101 4_1:+85590169 Msa0580490:CDS
GCTTGCAATCCTTGCATCCC+GGG 0.466766 4_1:-85590112 None:intergenic
ACTTGCATGTTGTTAAATGC+AGG 0.468024 4_1:+85588743 Msa0580490:CDS
GTACTTATTCAAATCAGTGC+TGG 0.468781 4_1:+85588956 Msa0580490:CDS
AAGCCGTACATAACATTTCA+AGG 0.471438 4_1:+85589224 Msa0580490:CDS
GTGTACAAATGTTGGGGACC+AGG 0.473026 4_1:+85591551 Msa0580490:CDS
GGCTTGCAATCCTTGCATCC+CGG 0.476597 4_1:-85590113 None:intergenic
CATTCAATAGCAACAAGGTT+CGG 0.477326 4_1:+85591204 Msa0580490:CDS
CATGATAGAGCTTGTGATCC+TGG 0.477605 4_1:+85589278 Msa0580490:CDS
GTACGTAACTGTTGTCCGTT+GGG 0.482645 4_1:-85589302 None:intergenic
AGAACACTCAATAAAAGAAT+CGG 0.482751 4_1:-85591873 None:intergenic
GGCAGTACGTGGGGTGTCAT+GGG 0.482778 4_1:+85591580 Msa0580490:CDS
TTCAAGGAATGCTACATTGA+GGG 0.487345 4_1:+85590232 Msa0580490:CDS
TACTATATATCTTGGGAGCA+TGG 0.488535 4_1:-85588588 None:intergenic
GTAATCACAGTTGATATTAA+CGG 0.490404 4_1:+85588869 Msa0580490:CDS
TGAAGGTAGTGTGGTGATGA+TGG 0.492113 4_1:-85588787 None:intergenic
GGGGACAAAGCATACTTCTC+CGG 0.500164 4_1:+85590154 Msa0580490:CDS
TTACGGCGTCTTGGACGGAC+TGG 0.502096 4_1:-85588904 None:intergenic
GGTGTCATGGGCACGAGAGT+TGG 0.502489 4_1:+85591592 Msa0580490:CDS
TCTTTGGAGTGTACAAATGT+TGG 0.504995 4_1:+85591543 Msa0580490:CDS
ATCAGGAAGAGCTTCGTCAA+TGG 0.508046 4_1:+85591638 Msa0580490:CDS
TACATAACATTTCAAGGGGA+AGG 0.513154 4_1:+85589230 Msa0580490:CDS
TGCACCATGGAAGCCACAGC+CGG 0.513222 4_1:-85590173 None:intergenic
AACTGTTGTCCGTTGGGACC+AGG 0.513658 4_1:-85589296 None:intergenic
ACTATATGTAGGACGTGCAA+TGG 0.515008 4_1:+85591311 Msa0580490:CDS
TATAGTTGCACACGGTGGTT+GGG 0.519022 4_1:+85591377 Msa0580490:CDS
GTTATGTACGGCTTTGTCAC+GGG 0.520170 4_1:-85589215 None:intergenic
ATATAGTTGCACACGGTGGT+TGG 0.521044 4_1:+85591376 Msa0580490:CDS
GCACCAATGCCCGGGATGCA+AGG 0.523172 4_1:+85590103 Msa0580490:CDS
TTCGTACGATGCAAGGTGAC+AGG 0.524057 4_1:+85591279 Msa0580490:CDS
AAAGCATACTTCTCCGGCTG+TGG 0.525074 4_1:+85590160 Msa0580490:CDS
CAGCGCCGGCACCAATGCCC+GGG 0.525664 4_1:+85590095 Msa0580490:CDS
TGAAGCCTGTCTTCTCGTCT+GGG 0.532273 4_1:-85591254 None:intergenic
CCAGGAGCAGAGGCAGTACG+TGG 0.532749 4_1:+85591569 Msa0580490:CDS
GACACGCTTTGCGACGATGC+TGG 0.538120 4_1:+85590199 Msa0580490:CDS
GTTCGGATCCATAGCAGCAC+AGG 0.542754 4_1:+85591221 Msa0580490:CDS
GTGATTACACGGTTACCGAC+AGG 0.543167 4_1:-85588854 None:intergenic
GATCCATAGCAGCACAGGAT+AGG 0.554332 4_1:+85591226 Msa0580490:CDS
TGTTATGTACGGCTTTGTCA+CGG 0.554628 4_1:-85589216 None:intergenic
CAATACCCAGACGAGAAGAC+AGG 0.556786 4_1:+85591249 Msa0580490:CDS
CCCTGAAATGCGAAACGCCA+CGG 0.559239 4_1:-85590134 None:intergenic
GCTTCGTCAATGGGAGACAT+TGG 0.566621 4_1:+85591648 Msa0580490:CDS
AACTTGATGGTGTAGAGAGA+AGG 0.569823 4_1:+85589184 Msa0580490:intron
TTTGGAGTGTACAAATGTTG+GGG 0.570214 4_1:+85591545 Msa0580490:CDS
AGTACGTAACTGTTGTCCGT+TGG 0.570235 4_1:-85589303 None:intergenic
CTTCAAGGAATGCTACATTG+AGG 0.571058 4_1:+85590231 Msa0580490:CDS
CAAACTCATCCAAGAACCAA+TGG 0.573843 4_1:+85588825 Msa0580490:CDS
ACAGTTGATATTAACGGTGG+AGG 0.587474 4_1:+85588875 Msa0580490:CDS
TTCTTTATTACAATTGAAGG+AGG 0.588089 4_1:-85591900 None:intergenic
CTTCACATTCGTACGATGCA+AGG 0.588588 4_1:+85591272 Msa0580490:CDS
AATGAATCATACCTTGTACA+TGG 0.588920 4_1:-85590287 None:intergenic
GAGACATTGGATCGCACCTA+CGG 0.590531 4_1:+85591661 Msa0580490:CDS
AGCCGTACATAACATTTCAA+GGG 0.591308 4_1:+85589225 Msa0580490:CDS
ACATAACATTTCAAGGGGAA+GGG 0.591823 4_1:+85589231 Msa0580490:CDS
TGCAAAGAATGTAAAAGAGT+AGG 0.602823 4_1:-85588704 None:intergenic
TAATATCAACTGTGATTACA+CGG 0.603000 4_1:-85588865 None:intergenic
ATGTTGGGGACCAGGAGCAG+AGG 0.612194 4_1:+85591559 Msa0580490:CDS
CGTGGCGTTTCGCATTTCAG+GGG 0.614191 4_1:+85590135 Msa0580490:CDS
AGGAGCAGAGGCAGTACGTG+GGG 0.618933 4_1:+85591571 Msa0580490:CDS
GCTTGTGATCCTGGTCCCAA+CGG 0.619512 4_1:+85589287 Msa0580490:CDS
AAATGGCAGATCCATGTACA+AGG 0.625424 4_1:+85590276 Msa0580490:CDS
TTGATGGTGTAGAGAGAAGG+TGG 0.630741 4_1:+85589187 Msa0580490:intron
CGCTTTGCGACGATGCTGGT+AGG 0.633544 4_1:+85590203 Msa0580490:CDS
ATATATGGCTAAGCATCCGT+AGG 0.635104 4_1:-85591677 None:intergenic
GCCGTACATAACATTTCAAG+GGG 0.638381 4_1:+85589226 Msa0580490:CDS
GAGCATGTAGAATACAGCGC+CGG 0.642880 4_1:+85590081 Msa0580490:intron
TTGCACGTCCTACATATAGT+GGG 0.643315 4_1:-85591308 None:intergenic
ACAATGTATTGAAGGTAGTG+TGG 0.646502 4_1:-85588796 None:intergenic
CAGGAGCAGAGGCAGTACGT+GGG 0.662580 4_1:+85591570 Msa0580490:CDS
AACCAATGGATCGAACCTGT+CGG 0.668325 4_1:+85588839 Msa0580490:CDS
GATGCAAGGATTGCAAGCCG+TGG 0.671605 4_1:+85590117 Msa0580490:CDS
GGCTCAGGCCCACTATATGT+AGG 0.671680 4_1:+85591300 Msa0580490:CDS
ATCACAGTTGATATTAACGG+TGG 0.676561 4_1:+85588872 Msa0580490:CDS
AGCTGCATTCAATAGCAACA+AGG 0.679636 4_1:+85591199 Msa0580490:CDS
TTTGATGATATAGTTGCACA+CGG 0.696006 4_1:+85591369 Msa0580490:CDS
AAAGCGTGTCTTGTGCACCA+TGG 0.707579 4_1:-85590186 None:intergenic
ACAACAGTCTCTCCGCAGAG+AGG 0.708082 4_1:-85591837 None:intergenic
TAATATAGTTATCCTCTCTG+CGG 0.714189 4_1:+85591825 Msa0580490:three_prime_UTR
TATATGTAGGACGTGCAATG+GGG 0.736057 4_1:+85591313 Msa0580490:CDS
ATGGTGTAGAGAGAAGGTGG+TGG 0.744314 4_1:+85589190 Msa0580490:intron
GATGATATAGTTGCACACGG+TGG 0.832583 4_1:+85591372 Msa0580490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATA+TGG + chr4_1:85589533-85589552 Msa0580490:intron 0.0%
!! ATTATTAAAAAATAATAAAA+AGG - chr4_1:85590725-85590744 None:intergenic 0.0%
!!! TTTTAATAATAGTACTTAAT+AGG + chr4_1:85590735-85590754 Msa0580490:intron 10.0%
!! GTATTTATACTATATATCTT+GGG - chr4_1:85588598-85588617 None:intergenic 15.0%
!! TGTATTTATACTATATATCT+TGG - chr4_1:85588599-85588618 None:intergenic 15.0%
!! GTATTAATTCTTAATATTGT+TGG + chr4_1:85589107-85589126 Msa0580490:intron 15.0%
!! TTTGAATGAAAGTAATAATT+TGG + chr4_1:85589150-85589169 Msa0580490:intron 15.0%
!! TATATTTACAATGACAATAT+TGG - chr4_1:85589509-85589528 None:intergenic 15.0%
!! TAGTTTATTTATCTCAATTA+TGG + chr4_1:85590336-85590355 Msa0580490:intron 15.0%
!!! ATGATTATTTTATTGAAAAG+TGG + chr4_1:85590855-85590874 Msa0580490:intron 15.0%
!! TCCAATGTAAAAAAAAATTA+GGG - chr4_1:85590902-85590921 None:intergenic 15.0%
!! TCAACAAAAAAAAAAAAAAC+AGG - chr4_1:85591001-85591020 None:intergenic 15.0%
!! TAATAAAAACATCTCTTAAT+TGG + chr4_1:85591159-85591178 Msa0580490:intron 15.0%
!! TAATAAAGAAATGCTTATAA+AGG + chr4_1:85591913-85591932 Msa0580490:three_prime_UTR 15.0%
!!! TTTGTGTATTTTATAGTAAG+AGG - chr4_1:85588491-85588510 None:intergenic 20.0%
!! ACAAATAAGAATAGAATGTT+TGG - chr4_1:85588649-85588668 None:intergenic 20.0%
!! CTTAATTAATGCTAAAGAAA+AGG - chr4_1:85589057-85589076 None:intergenic 20.0%
!! TATACTAATATTTACCTTGA+AGG - chr4_1:85589384-85589403 None:intergenic 20.0%
!!! AGAATTTTTCAAGAAATAGT+TGG + chr4_1:85589641-85589660 Msa0580490:intron 20.0%
!!! AATAGTTGGCAAATTTTAAT+TGG + chr4_1:85589655-85589674 Msa0580490:intron 20.0%
!! ATTTCCAAAAATGAAATCAA+TGG - chr4_1:85590260-85590279 None:intergenic 20.0%
!!! ATTGATTTCATTTTTGGAAA+TGG + chr4_1:85590259-85590278 Msa0580490:CDS 20.0%
!! TAACATCAAAATTCATTTAG+TGG + chr4_1:85590451-85590470 Msa0580490:intron 20.0%
!! CCAATGTAAAAAAAAATTAG+GGG - chr4_1:85590901-85590920 None:intergenic 20.0%
!! CTCCAATGTAAAAAAAAATT+AGG - chr4_1:85590903-85590922 None:intergenic 20.0%
!!! CTAATTTTTTTTTACATTGG+AGG + chr4_1:85590901-85590920 Msa0580490:intron 20.0%
!! AAACAATAAGAAGGATAAAA+AGG - chr4_1:85591438-85591457 None:intergenic 20.0%
!!! TTTTATCCTTCTTATTGTTT+TGG + chr4_1:85591438-85591457 Msa0580490:intron 20.0%
!! CAAGAATAATGCTATATATA+TGG - chr4_1:85591695-85591714 None:intergenic 20.0%
!!! ATTTTTGTTCTTTTCAACAT+AGG + chr4_1:85591788-85591807 Msa0580490:three_prime_UTR 20.0%
!! CATTTCTTTATTACAATTGA+AGG - chr4_1:85591906-85591925 None:intergenic 20.0%
!!! ATTTTATAGTAAGAGGCATT+TGG - chr4_1:85588484-85588503 None:intergenic 25.0%
! TACAATGTGTGTAAGTAAAA+AGG - chr4_1:85588533-85588552 None:intergenic 25.0%
! CATGGTGATTAAAATATTTG+TGG - chr4_1:85588573-85588592 None:intergenic 25.0%
! TAATATCAACTGTGATTACA+CGG - chr4_1:85588868-85588887 None:intergenic 25.0%
!! GTAATCACAGTTGATATTAA+CGG + chr4_1:85588869-85588888 Msa0580490:CDS 25.0%
! CATTCATTGTATTGTTATCT+GGG - chr4_1:85588937-85588956 None:intergenic 25.0%
! ACATTCATTGTATTGTTATC+TGG - chr4_1:85588938-85588957 None:intergenic 25.0%
! GAATGAAAGTAATAATTTGG+TGG + chr4_1:85589153-85589172 Msa0580490:intron 25.0%
! TAACTGATAATTAACCTCAA+TGG + chr4_1:85589442-85589461 Msa0580490:intron 25.0%
! AACTGATAATTAACCTCAAT+GGG + chr4_1:85589443-85589462 Msa0580490:intron 25.0%
! TTTACAATGACAATATTGGT+AGG - chr4_1:85589505-85589524 None:intergenic 25.0%
! TAAGCTAGAGAAGTTAAAAA+AGG - chr4_1:85589988-85590007 None:intergenic 25.0%
!! TTTTACCTAAGTTAAAAAGC+TGG - chr4_1:85590011-85590030 None:intergenic 25.0%
!! TATTTTATTGAAAAGTGGTG+TGG + chr4_1:85590860-85590879 Msa0580490:intron 25.0%
!! ATTTTATTGAAAAGTGGTGT+GGG + chr4_1:85590861-85590880 Msa0580490:intron 25.0%
!!! CCCCTAATTTTTTTTTACAT+TGG + chr4_1:85590898-85590917 Msa0580490:intron 25.0%
!!! GAGATGTTTTTATTAAGAGA+TGG - chr4_1:85591154-85591173 None:intergenic 25.0%
! ATTTGTGGTGAAACTTAATT+AGG + chr4_1:85591513-85591532 Msa0580490:intron 25.0%
!! TTCCTTTAAAAATGTGAGTA+GGG - chr4_1:85591722-85591741 None:intergenic 25.0%
!! TTTCCTTTAAAAATGTGAGT+AGG - chr4_1:85591723-85591742 None:intergenic 25.0%
! AGAACACTCAATAAAAGAAT+CGG - chr4_1:85591876-85591895 None:intergenic 25.0%
! TTCTTTATTACAATTGAAGG+AGG - chr4_1:85591903-85591922 None:intergenic 25.0%
AAAAAGGAAGAGAAAATGAG+AGG - chr4_1:85588517-85588536 None:intergenic 30.0%
CAATTCTCAACTCTCTATAA+TGG + chr4_1:85588676-85588695 Msa0580490:exon 30.0%
TGCAAAGAATGTAAAAGAGT+AGG - chr4_1:85588707-85588726 None:intergenic 30.0%
! ACATTCTTTGCAATTGCTTT+TGG + chr4_1:85588715-85588734 Msa0580490:CDS 30.0%
TGTAGTAGACAATGTATTGA+AGG - chr4_1:85588807-85588826 None:intergenic 30.0%
! TAACTTGCACCATTATTTAG+TGG + chr4_1:85589020-85589039 Msa0580490:intron 30.0%
CATGTAACACCACTAAATAA+TGG - chr4_1:85589032-85589051 None:intergenic 30.0%
GAGAAATAACAGTGATTGAA+TGG + chr4_1:85589255-85589274 Msa0580490:CDS 30.0%
ATTCTTTGCAGAGAAATAGT+TGG - chr4_1:85589357-85589376 None:intergenic 30.0%
CTTTCATTTCAATGTAAGAG+TGG + chr4_1:85589938-85589957 Msa0580490:intron 30.0%
AATGAATCATACCTTGTACA+TGG - chr4_1:85590290-85590309 None:intergenic 30.0%
TGGCAACATTACCTTAATAA+TGG + chr4_1:85590356-85590375 Msa0580490:intron 30.0%
GGCAACATTACCTTAATAAT+GGG + chr4_1:85590357-85590376 Msa0580490:intron 30.0%
CTTTAACGAACCCATTATTA+AGG - chr4_1:85590370-85590389 None:intergenic 30.0%
AGTGTGACAGAGAAATAAAA+AGG - chr4_1:85590416-85590435 None:intergenic 30.0%
ACAGGGATACTAATAAAGTA+AGG + chr4_1:85590496-85590515 Msa0580490:intron 30.0%
TTGCCAAGTGTTTATGAATA+TGG + chr4_1:85590522-85590541 Msa0580490:intron 30.0%
AGTCCATATTCATAAACACT+TGG - chr4_1:85590528-85590547 None:intergenic 30.0%
ACATATATTAGTTCAATGCC+TGG - chr4_1:85590617-85590636 None:intergenic 30.0%
! TTTTGATAGATGATCTCCAT+TGG - chr4_1:85590773-85590792 None:intergenic 30.0%
AATGGAGATCATCTATCAAA+AGG + chr4_1:85590772-85590791 Msa0580490:intron 30.0%
! TTTTATTGAAAAGTGGTGTG+GGG + chr4_1:85590862-85590881 Msa0580490:intron 30.0%
!! AAAATTAGGGGTATCTTAAG+TGG - chr4_1:85590889-85590908 None:intergenic 30.0%
! TTTGATGATATAGTTGCACA+CGG + chr4_1:85591369-85591388 Msa0580490:CDS 30.0%
TCATCACCAAAACAATAAGA+AGG - chr4_1:85591447-85591466 None:intergenic 30.0%
TAAAGTTCAGAAAATCAGTG+TGG - chr4_1:85591481-85591500 None:intergenic 30.0%
!! TTAACTGTCTTGTTGATTTG+TGG + chr4_1:85591498-85591517 Msa0580490:intron 30.0%
TTAATTAGGACTGTGTTCTT+TGG + chr4_1:85591527-85591546 Msa0580490:intron 30.0%
! TTCCCTACTCACATTTTTAA+AGG + chr4_1:85591717-85591736 Msa0580490:three_prime_UTR 30.0%
! ATATGACAACTCTTTTGCAT+GGG - chr4_1:85591770-85591789 None:intergenic 30.0%
! AATATGACAACTCTTTTGCA+TGG - chr4_1:85591771-85591790 None:intergenic 30.0%
TAATATAGTTATCCTCTCTG+CGG + chr4_1:85591825-85591844 Msa0580490:three_prime_UTR 30.0%
TACTATATATCTTGGGAGCA+TGG - chr4_1:85588591-85588610 None:intergenic 35.0%
ACTTGCATGTTGTTAAATGC+AGG + chr4_1:85588743-85588762 Msa0580490:CDS 35.0%
ACAATGTATTGAAGGTAGTG+TGG - chr4_1:85588799-85588818 None:intergenic 35.0%
! ATCACAGTTGATATTAACGG+TGG + chr4_1:85588872-85588891 Msa0580490:CDS 35.0%
! GTTATCTGGGATGGAATTTA+CGG - chr4_1:85588924-85588943 None:intergenic 35.0%
CATTGTATTGTTATCTGGGA+TGG - chr4_1:85588933-85588952 None:intergenic 35.0%
GTACTTATTCAAATCAGTGC+TGG + chr4_1:85588956-85588975 Msa0580490:CDS 35.0%
AAGCCGTACATAACATTTCA+AGG + chr4_1:85589224-85589243 Msa0580490:CDS 35.0%
AGCCGTACATAACATTTCAA+GGG + chr4_1:85589225-85589244 Msa0580490:CDS 35.0%
TCCCCTTGAAATGTTATGTA+CGG - chr4_1:85589230-85589249 None:intergenic 35.0%
TACATAACATTTCAAGGGGA+AGG + chr4_1:85589230-85589249 Msa0580490:CDS 35.0%
ACATAACATTTCAAGGGGAA+GGG + chr4_1:85589231-85589250 Msa0580490:CDS 35.0%
TGCAAAGAATATCACCTTCA+AGG + chr4_1:85589367-85589386 Msa0580490:CDS 35.0%
ATTAACCTCAATGGGAACAA+AGG + chr4_1:85589451-85589470 Msa0580490:intron 35.0%
TTAACCTCAATGGGAACAAA+GGG + chr4_1:85589452-85589471 Msa0580490:intron 35.0%
AAAACAAGTGAAAGATCACG+AGG - chr4_1:85589869-85589888 None:intergenic 35.0%
!!! GCTTACCAGCTTTTTAACTT+AGG + chr4_1:85590003-85590022 Msa0580490:intron 35.0%
TTCAAGGAATGCTACATTGA+GGG + chr4_1:85590232-85590251 Msa0580490:CDS 35.0%
!!! GGCTCCATTGATTTCATTTT+TGG + chr4_1:85590253-85590272 Msa0580490:CDS 35.0%
AAAACATTGAGATGCGTACA+GGG + chr4_1:85590479-85590498 Msa0580490:intron 35.0%
! GGATGCTCTAATGTTCTTTT+GGG - chr4_1:85590648-85590667 None:intergenic 35.0%
! TGGATGCTCTAATGTTCTTT+TGG - chr4_1:85590649-85590668 None:intergenic 35.0%
! CTCACATTTGAGGTCTTAAA+CGG + chr4_1:85590680-85590699 Msa0580490:intron 35.0%
! TCACATTTGAGGTCTTAAAC+GGG + chr4_1:85590681-85590700 Msa0580490:intron 35.0%
AAAAGGTGATGTGTTCATGT+GGG - chr4_1:85590708-85590727 None:intergenic 35.0%
AAAAAGGTGATGTGTTCATG+TGG - chr4_1:85590709-85590728 None:intergenic 35.0%
!! TTACATTGGAGGTACCTATT+AGG + chr4_1:85590912-85590931 Msa0580490:intron 35.0%
! ACTCTTTACCATTTAAGACC+CGG - chr4_1:85590940-85590959 None:intergenic 35.0%
! ATGGAGTAGTTTGACTTTGA+CGG - chr4_1:85591135-85591154 None:intergenic 35.0%
TCTCTTAATTGGAGATTCTC+AGG + chr4_1:85591170-85591189 Msa0580490:intron 35.0%
CATTCAATAGCAACAAGGTT+CGG + chr4_1:85591204-85591223 Msa0580490:CDS 35.0%
!!! ACTTGTTTTTGTTGTTAGCG+TGG - chr4_1:85591413-85591432 None:intergenic 35.0%
!! TCTTTGGAGTGTACAAATGT+TGG + chr4_1:85591543-85591562 Msa0580490:CDS 35.0%
!! CTTTGGAGTGTACAAATGTT+GGG + chr4_1:85591544-85591563 Msa0580490:CDS 35.0%
! TTTGGAGTGTACAAATGTTG+GGG + chr4_1:85591545-85591564 Msa0580490:CDS 35.0%
AAACAGCACATCCATTTATC+AGG + chr4_1:85591621-85591640 Msa0580490:CDS 35.0%
CAAACTCATCCAAGAACCAA+TGG + chr4_1:85588825-85588844 Msa0580490:CDS 40.0%
! ACAGTTGATATTAACGGTGG+AGG + chr4_1:85588875-85588894 Msa0580490:CDS 40.0%
! AGTGCTGGATTCTACAAGTA+TGG + chr4_1:85588971-85588990 Msa0580490:intron 40.0%
GGTGGTGCATATAAACTTGA+TGG + chr4_1:85589171-85589190 Msa0580490:intron 40.0%
!! AACTTGATGGTGTAGAGAGA+AGG + chr4_1:85589184-85589203 Msa0580490:intron 40.0%
! TGTTATGTACGGCTTTGTCA+CGG - chr4_1:85589219-85589238 None:intergenic 40.0%
GCCGTACATAACATTTCAAG+GGG + chr4_1:85589226-85589245 Msa0580490:CDS 40.0%
TGTGGCTGAAAAATAGGAAG+TGG - chr4_1:85589694-85589713 None:intergenic 40.0%
CTATTTGCCACAATTCAAGG+AGG - chr4_1:85589717-85589736 None:intergenic 40.0%
GCACTATTTGCCACAATTCA+AGG - chr4_1:85589720-85589739 None:intergenic 40.0%
GGCAAATAGTGCATTGTGAT+TGG + chr4_1:85589728-85589747 Msa0580490:intron 40.0%
CTTCAAGGAATGCTACATTG+AGG + chr4_1:85590231-85590250 Msa0580490:CDS 40.0%
AAATGGCAGATCCATGTACA+AGG + chr4_1:85590276-85590295 Msa0580490:CDS 40.0%
! CTGTACGCATCTCAATGTTT+TGG - chr4_1:85590480-85590499 None:intergenic 40.0%
CAAAACATTGAGATGCGTAC+AGG + chr4_1:85590478-85590497 Msa0580490:intron 40.0%
! CTACCACGTTTTTAATGACC+AGG + chr4_1:85590596-85590615 Msa0580490:intron 40.0%
ATGCCTGGTCATTAAAAACG+TGG - chr4_1:85590602-85590621 None:intergenic 40.0%
TAGGTACTCAACTACTCCAA+TGG + chr4_1:85590754-85590773 Msa0580490:intron 40.0%
! TTTAAGACCCGGTACCTAAT+AGG - chr4_1:85590929-85590948 None:intergenic 40.0%
!! ATTAGGTACCGGGTCTTAAA+TGG + chr4_1:85590929-85590948 Msa0580490:intron 40.0%
GGGTCTTAAATGGTAAAGAG+TGG + chr4_1:85590939-85590958 Msa0580490:intron 40.0%
AGCTGCATTCAATAGCAACA+AGG + chr4_1:85591199-85591218 Msa0580490:CDS 40.0%
TTGCACGTCCTACATATAGT+GGG - chr4_1:85591311-85591330 None:intergenic 40.0%
ATTGCACGTCCTACATATAG+TGG - chr4_1:85591312-85591331 None:intergenic 40.0%
ACTATATGTAGGACGTGCAA+TGG + chr4_1:85591311-85591330 Msa0580490:CDS 40.0%
CTATATGTAGGACGTGCAAT+GGG + chr4_1:85591312-85591331 Msa0580490:CDS 40.0%
TATATGTAGGACGTGCAATG+GGG + chr4_1:85591313-85591332 Msa0580490:CDS 40.0%
ACGAAGCTCTTCCTGATAAA+TGG - chr4_1:85591635-85591654 None:intergenic 40.0%
ATATATGGCTAAGCATCCGT+AGG - chr4_1:85591680-85591699 None:intergenic 40.0%
! TGAAGGTAGTGTGGTGATGA+TGG - chr4_1:85588790-85588809 None:intergenic 45.0%
!! AGGTTCGATCCATTGGTTCT+TGG - chr4_1:85588837-85588856 None:intergenic 45.0%
AACCAATGGATCGAACCTGT+CGG + chr4_1:85588839-85588858 Msa0580490:CDS 45.0%
!! TTGATGGTGTAGAGAGAAGG+TGG + chr4_1:85589187-85589206 Msa0580490:intron 45.0%
! GTTATGTACGGCTTTGTCAC+GGG - chr4_1:85589218-85589237 None:intergenic 45.0%
CATGATAGAGCTTGTGATCC+TGG + chr4_1:85589278-85589297 Msa0580490:CDS 45.0%
GTACGTAACTGTTGTCCGTT+GGG - chr4_1:85589305-85589324 None:intergenic 45.0%
AGTACGTAACTGTTGTCCGT+TGG - chr4_1:85589306-85589325 None:intergenic 45.0%
! AGGAGGTGTGGCTGAAAAAT+AGG - chr4_1:85589700-85589719 None:intergenic 45.0%
TGCTGGTAGGCATTACTTCA+AGG + chr4_1:85590216-85590235 Msa0580490:CDS 45.0%
! GAACATTAGAGCATCCACGA+TGG + chr4_1:85590652-85590671 Msa0580490:intron 45.0%
AAATGTGAGAGTCTCCATCG+TGG - chr4_1:85590669-85590688 None:intergenic 45.0%
GATGGAGACTCTCACATTTG+AGG + chr4_1:85590670-85590689 Msa0580490:intron 45.0%
!! TGGAGGTACCTATTAGGTAC+CGG + chr4_1:85590918-85590937 Msa0580490:intron 45.0%
CTTCACATTCGTACGATGCA+AGG + chr4_1:85591272-85591291 Msa0580490:CDS 45.0%
GATGATATAGTTGCACACGG+TGG + chr4_1:85591372-85591391 Msa0580490:CDS 45.0%
ATATAGTTGCACACGGTGGT+TGG + chr4_1:85591376-85591395 Msa0580490:CDS 45.0%
TATAGTTGCACACGGTGGTT+GGG + chr4_1:85591377-85591396 Msa0580490:CDS 45.0%
ATCAGGAAGAGCTTCGTCAA+TGG + chr4_1:85591638-85591657 Msa0580490:CDS 45.0%
TCAGGAAGAGCTTCGTCAAT+GGG + chr4_1:85591639-85591658 Msa0580490:CDS 45.0%
TACCGACAGGTTCGATCCAT+TGG - chr4_1:85588844-85588863 None:intergenic 50.0%
GTGATTACACGGTTACCGAC+AGG - chr4_1:85588857-85588876 None:intergenic 50.0%
GGAATTTACGGCGTCTTGGA+CGG - chr4_1:85588912-85588931 None:intergenic 50.0%
GGATGGAATTTACGGCGTCT+TGG - chr4_1:85588916-85588935 None:intergenic 50.0%
ATGGTGTAGAGAGAAGGTGG+TGG + chr4_1:85589190-85589209 Msa0580490:intron 50.0%
ACCTCAATGGGAACAAAGGG+AGG + chr4_1:85589455-85589474 Msa0580490:intron 50.0%
TCCTCCCTTTGTTCCCATTG+AGG - chr4_1:85589459-85589478 None:intergenic 50.0%
CAATGGGAACAAAGGGAGGA+AGG + chr4_1:85589459-85589478 Msa0580490:intron 50.0%
AGCCACACCTCCTTGAATTG+TGG + chr4_1:85589707-85589726 Msa0580490:intron 50.0%
TGCCACAATTCAAGGAGGTG+TGG - chr4_1:85589712-85589731 None:intergenic 50.0%
GAGCATGTAGAATACAGCGC+CGG + chr4_1:85590081-85590100 Msa0580490:intron 50.0%
GGGGACAAAGCATACTTCTC+CGG + chr4_1:85590154-85590173 Msa0580490:CDS 50.0%
AAAGCATACTTCTCCGGCTG+TGG + chr4_1:85590160-85590179 Msa0580490:CDS 50.0%
AAAGCGTGTCTTGTGCACCA+TGG - chr4_1:85590189-85590208 None:intergenic 50.0%
!! GGAGGTACCTATTAGGTACC+GGG + chr4_1:85590919-85590938 Msa0580490:intron 50.0%
GATCCATAGCAGCACAGGAT+AGG + chr4_1:85591226-85591245 Msa0580490:CDS 50.0%
! TTGCCTATCCTGTGCTGCTA+TGG - chr4_1:85591232-85591251 None:intergenic 50.0%
CAATACCCAGACGAGAAGAC+AGG + chr4_1:85591249-85591268 Msa0580490:CDS 50.0%
TGAAGCCTGTCTTCTCGTCT+GGG - chr4_1:85591257-85591276 None:intergenic 50.0%
TTCGTACGATGCAAGGTGAC+AGG + chr4_1:85591279-85591298 Msa0580490:CDS 50.0%
ACACGGTGGTTGGGATGATT+GGG + chr4_1:85591386-85591405 Msa0580490:CDS 50.0%
GTGTACAAATGTTGGGGACC+AGG + chr4_1:85591551-85591570 Msa0580490:CDS 50.0%
GCTTCGTCAATGGGAGACAT+TGG + chr4_1:85591648-85591667 Msa0580490:CDS 50.0%
GAGACATTGGATCGCACCTA+CGG + chr4_1:85591661-85591680 Msa0580490:CDS 50.0%
GCTTGTGATCCTGGTCCCAA+CGG + chr4_1:85589287-85589306 Msa0580490:CDS 55.0%
AACTGTTGTCCGTTGGGACC+AGG - chr4_1:85589299-85589318 None:intergenic 55.0%
AATCCTTGCATCCCGGGCAT+TGG - chr4_1:85590109-85590128 None:intergenic 55.0%
GCTTGCAATCCTTGCATCCC+GGG - chr4_1:85590115-85590134 None:intergenic 55.0%
GGCTTGCAATCCTTGCATCC+CGG - chr4_1:85590116-85590135 None:intergenic 55.0%
GATGCAAGGATTGCAAGCCG+TGG + chr4_1:85590117-85590136 Msa0580490:CDS 55.0%
CCCTGAAATGCGAAACGCCA+CGG - chr4_1:85590137-85590156 None:intergenic 55.0%
CCGTGGCGTTTCGCATTTCA+GGG + chr4_1:85590134-85590153 Msa0580490:CDS 55.0%
CGTGGCGTTTCGCATTTCAG+GGG + chr4_1:85590135-85590154 Msa0580490:CDS 55.0%
GTTCGGATCCATAGCAGCAC+AGG + chr4_1:85591221-85591240 Msa0580490:CDS 55.0%
GTGAAGCCTGTCTTCTCGTC+TGG - chr4_1:85591258-85591277 None:intergenic 55.0%
GGCTCAGGCCCACTATATGT+AGG + chr4_1:85591300-85591319 Msa0580490:CDS 55.0%
CACACGGTGGTTGGGATGAT+TGG + chr4_1:85591385-85591404 Msa0580490:CDS 55.0%
ACAACAGTCTCTCCGCAGAG+AGG - chr4_1:85591840-85591859 None:intergenic 55.0%
!! CGTCTTGGACGGACTGGAAC+TGG - chr4_1:85588901-85588920 None:intergenic 60.0%
TTACGGCGTCTTGGACGGAC+TGG - chr4_1:85588907-85588926 None:intergenic 60.0%
GCCGTGGCGTTTCGCATTTC+AGG + chr4_1:85590133-85590152 Msa0580490:CDS 60.0%
TTCTCCGGCTGTGGCTTCCA+TGG + chr4_1:85590169-85590188 Msa0580490:CDS 60.0%
TGCACCATGGAAGCCACAGC+CGG - chr4_1:85590176-85590195 None:intergenic 60.0%
GACACGCTTTGCGACGATGC+TGG + chr4_1:85590199-85590218 Msa0580490:CDS 60.0%
CGCTTTGCGACGATGCTGGT+AGG + chr4_1:85590203-85590222 Msa0580490:CDS 60.0%
CGATGCAAGGTGACAGGCTC+AGG + chr4_1:85591285-85591304 Msa0580490:CDS 60.0%
ATGTTGGGGACCAGGAGCAG+AGG + chr4_1:85591559-85591578 Msa0580490:CDS 60.0%
CAGGAGCAGAGGCAGTACGT+GGG + chr4_1:85591570-85591589 Msa0580490:CDS 60.0%
AGGAGCAGAGGCAGTACGTG+GGG + chr4_1:85591571-85591590 Msa0580490:CDS 60.0%
! AGGCAGTACGTGGGGTGTCA+TGG + chr4_1:85591579-85591598 Msa0580490:CDS 60.0%
! GGCAGTACGTGGGGTGTCAT+GGG + chr4_1:85591580-85591599 Msa0580490:CDS 60.0%
! GGTGTCATGGGCACGAGAGT+TGG + chr4_1:85591592-85591611 Msa0580490:CDS 60.0%
!! TGCATCCCGGGCATTGGTGC+CGG - chr4_1:85590103-85590122 None:intergenic 65.0%
GCACCAATGCCCGGGATGCA+AGG + chr4_1:85590103-85590122 Msa0580490:CDS 65.0%
CCACGTACTGCCTCTGCTCC+TGG - chr4_1:85591572-85591591 None:intergenic 65.0%
CCAGGAGCAGAGGCAGTACG+TGG + chr4_1:85591569-85591588 Msa0580490:CDS 65.0%
!! ACAGCGCCGGCACCAATGCC+CGG + chr4_1:85590094-85590113 Msa0580490:CDS 70.0%
!! CAGCGCCGGCACCAATGCCC+GGG + chr4_1:85590095-85590114 Msa0580490:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 85588480 85591965 85588480 ID=Msa0580490;Name=Msa0580490
chr4_1 mRNA 85588480 85591965 85588480 ID=Msa0580490-mRNA-1;Parent=Msa0580490;Name=Msa0580490-mRNA-1;_AED=0.03;_eAED=0.03;_QI=215|1|1|1|1|1|5|274|361
chr4_1 exon 85588480 85588987 85588480 ID=Msa0580490-mRNA-1:exon:31482;Parent=Msa0580490-mRNA-1
chr4_1 exon 85589199 85589388 85589199 ID=Msa0580490-mRNA-1:exon:31483;Parent=Msa0580490-mRNA-1
chr4_1 exon 85590091 85590297 85590091 ID=Msa0580490-mRNA-1:exon:31484;Parent=Msa0580490-mRNA-1
chr4_1 exon 85591192 85591430 85591192 ID=Msa0580490-mRNA-1:exon:31485;Parent=Msa0580490-mRNA-1
chr4_1 exon 85591535 85591965 85591535 ID=Msa0580490-mRNA-1:exon:31486;Parent=Msa0580490-mRNA-1
chr4_1 five_prime_UTR 85588480 85588694 85588480 ID=Msa0580490-mRNA-1:five_prime_utr;Parent=Msa0580490-mRNA-1
chr4_1 CDS 85588695 85588987 85588695 ID=Msa0580490-mRNA-1:cds;Parent=Msa0580490-mRNA-1
chr4_1 CDS 85589199 85589388 85589199 ID=Msa0580490-mRNA-1:cds;Parent=Msa0580490-mRNA-1
chr4_1 CDS 85590091 85590297 85590091 ID=Msa0580490-mRNA-1:cds;Parent=Msa0580490-mRNA-1
chr4_1 CDS 85591192 85591430 85591192 ID=Msa0580490-mRNA-1:cds;Parent=Msa0580490-mRNA-1
chr4_1 CDS 85591535 85591691 85591535 ID=Msa0580490-mRNA-1:cds;Parent=Msa0580490-mRNA-1
chr4_1 three_prime_UTR 85591692 85591965 85591692 ID=Msa0580490-mRNA-1:three_prime_utr;Parent=Msa0580490-mRNA-1
Gene Sequence

>Msa0580490

ATGGATACACCTACTCTTTTACATTCTTTGCAATTGCTTTTGGTTTTCACTTGCATGTTGTTAAATGCAGGAATAGTGACACTAGCTGCACGCCATCATCACCACACTACCTTCAATACATTGTCTACTACAAACTCATCCAAGAACCAATGGATCGAACCTGTCGGTAACCGTGTAATCACAGTTGATATTAACGGTGGAGGCCAGTTCCAGTCCGTCCAAGACGCCGTAAATTCCATCCCAGATAACAATACAATGAATGTACTTATTCAAATCAGTGCTGGATTCTACAAAGAGAAGGTGGTGGTTCCCGTGACAAAGCCGTACATAACATTTCAAGGGGAAGGGAGAGAAATAACAGTGATTGAATGGCATGATAGAGCTTGTGATCCTGGTCCCAACGGACAACAGTTACGTACTTATAGAACTGCTTCTGTTACTGTCTTTGCCAACTATTTCTCTGCAAAGAATATCACCTTCAAGAATACAGCGCCGGCACCAATGCCCGGGATGCAAGGATTGCAAGCCGTGGCGTTTCGCATTTCAGGGGACAAAGCATACTTCTCCGGCTGTGGCTTCCATGGTGCACAAGACACGCTTTGCGACGATGCTGGTAGGCATTACTTCAAGGAATGCTACATTGAGGGCTCCATTGATTTCATTTTTGGAAATGGCAGATCCATGTACAAGGATTGTGAGCTGCATTCAATAGCAACAAGGTTCGGATCCATAGCAGCACAGGATAGGCAATACCCAGACGAGAAGACAGGCTTCACATTCGTACGATGCAAGGTGACAGGCTCAGGCCCACTATATGTAGGACGTGCAATGGGGCAATACTCAAGAATAGTTTATGCATATACATACTTTGATGATATAGTTGCACACGGTGGTTGGGATGATTGGGACCACGCTAACAACAAAAACAAGACTGTGTTCTTTGGAGTGTACAAATGTTGGGGACCAGGAGCAGAGGCAGTACGTGGGGTGTCATGGGCACGAGAGTTGGATTTCGAAACAGCACATCCATTTATCAGGAAGAGCTTCGTCAATGGGAGACATTGGATCGCACCTACGGATGCTTAG
Protein sequence

>Msa0580490

MDTPTLLHSLQLLLVFTCMLLNAGIVTLAARHHHHTTFNTLSTTNSSKNQWIEPVGNRVITVDINGGGQFQSVQDAVNSIPDNNTMNVLIQISAGFYKEKVVVPVTKPYITFQGEGREITVIEWHDRACDPGPNGQQLRTYRTASVTVFANYFSAKNITFKNTAPAPMPGMQGLQAVAFRISGDKAYFSGCGFHGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRQYPDEKTGFTFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDIVAHGGWDDWDHANNKNKTVFFGVYKCWGPGAEAVRGVSWARELDFETAHPFIRKSFVNGRHWIAPTDA*