AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1132640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1132640 MtrunA17_Chr5g0399221 97.802 364 5 1 1 361 1 364 0.0 743
Msa1132640 MtrunA17_Chr6g0487101 52.941 289 135 1 68 356 93 380 8.82e-108 320
Msa1132640 MtrunA17_Chr8g0389771 53.265 291 133 2 68 356 88 377 6.18e-106 316
Msa1132640 MtrunA17_Chr7g0258341 47.869 305 144 4 60 356 90 387 7.53e-93 283
Msa1132640 MtrunA17_Chr5g0446301 46.599 294 151 4 66 356 67 357 5.75e-92 279
Msa1132640 MtrunA17_Chr7g0265201 44.575 341 163 8 25 356 62 385 1.53e-87 269
Msa1132640 MtrunA17_Chr1g0194311 39.335 361 201 6 12 356 10 368 5.86e-84 259
Msa1132640 MtrunA17_Chr4g0070051 42.857 308 155 7 58 358 52 345 1.18e-75 237
Msa1132640 MtrunA17_Chr2g0294321 40.656 305 165 4 57 356 53 346 1.01e-70 224
Msa1132640 MtrunA17_Chr7g0217941 40.323 310 165 7 59 357 5 305 2.48e-70 222
Msa1132640 MtrunA17_Chr6g0466961 36.741 313 179 6 50 356 25 324 3.52e-68 217
Msa1132640 MtrunA17_Chr6g0453551 38.835 309 171 6 59 357 5 305 1.09e-67 216
Msa1132640 MtrunA17_Chr6g0466951 37.700 313 176 6 50 356 54 353 4.70e-66 213
Msa1132640 MtrunA17_Chr4g0037751 33.894 357 218 6 12 358 15 363 2.00e-65 211
Msa1132640 MtrunA17_Chr5g0420831 34.746 354 215 5 13 358 18 363 2.18e-65 211
Msa1132640 MtrunA17_Chr1g0161731 35.632 348 201 7 16 356 8 339 5.82e-64 207
Msa1132640 MtrunA17_Chr2g0295851 39.016 305 161 5 57 356 52 336 9.33e-64 206
Msa1132640 MtrunA17_Chr8g0351871 37.013 308 171 6 58 357 31 323 1.62e-62 202
Msa1132640 MtrunA17_Chr5g0440871 35.065 308 174 6 56 357 32 319 1.93e-52 176
Msa1132640 MtrunA17_Chr7g0272141 30.769 364 222 7 14 359 183 534 1.04e-47 169
Msa1132640 MtrunA17_Chr4g0069881 31.447 318 192 6 57 359 221 527 5.05e-47 167
Msa1132640 MtrunA17_Chr6g0459781 44.828 174 93 2 59 232 12 182 6.24e-47 158
Msa1132640 MtrunA17_Chr8g0345281 32.063 315 187 6 60 359 279 581 3.96e-46 166
Msa1132640 MtrunA17_Chr8g0384121 32.476 311 177 7 68 356 254 553 4.11e-46 165
Msa1132640 MtrunA17_Chr1g0192331 31.366 322 184 9 60 359 214 520 1.54e-44 160
Msa1132640 MtrunA17_Chr1g0195301 32.484 314 179 8 65 359 248 547 7.90e-43 156
Msa1132640 MtrunA17_Chr1g0195321 32.484 314 179 8 65 359 248 547 7.90e-43 156
Msa1132640 MtrunA17_Chr4g0069841 30.032 313 192 6 62 359 208 508 8.69e-43 155
Msa1132640 MtrunA17_Chr2g0314691 39.000 200 119 3 161 358 5 203 9.28e-43 147
Msa1132640 MtrunA17_Chr1g0175661 31.132 318 190 7 59 359 245 550 1.34e-42 155
Msa1132640 MtrunA17_Chr4g0011771 31.790 324 191 8 50 359 198 505 3.37e-42 154
Msa1132640 MtrunA17_Chr4g0025841 36.000 250 138 4 58 304 35 265 4.17e-42 148
Msa1132640 MtrunA17_Chr4g0018371 32.595 316 186 9 60 359 227 531 4.45e-42 154
Msa1132640 MtrunA17_Chr7g0269531 31.546 317 185 8 59 356 251 554 5.61e-42 154
Msa1132640 MtrunA17_Chr7g0265161 36.486 222 115 5 140 357 14 213 9.75e-42 146
Msa1132640 MtrunA17_Chr1g0195331 32.166 314 180 8 65 359 248 547 1.23e-41 153
Msa1132640 MtrunA17_Chr3g0090091 30.380 316 193 8 60 359 216 520 2.01e-41 152
Msa1132640 MtrunA17_Chr3g0080391 30.769 312 194 6 60 361 269 568 6.39e-41 151
Msa1132640 MtrunA17_Chr3g0079341 31.469 286 172 7 60 332 215 489 7.58e-41 150
Msa1132640 MtrunA17_Chr3g0079361 30.063 316 194 8 60 359 215 519 9.19e-41 150
Msa1132640 MtrunA17_Chr3g0079351 29.747 316 195 8 60 359 215 519 9.67e-41 150
Msa1132640 MtrunA17_Chr7g0269511 29.787 329 201 8 49 359 240 556 1.65e-40 150
Msa1132640 MtrunA17_Chr8g0354901 30.449 312 195 6 60 361 276 575 2.89e-40 149
Msa1132640 MtrunA17_Chr2g0302021 29.560 318 197 6 57 359 212 517 3.43e-40 148
Msa1132640 MtrunA17_Chr7g0269521 29.375 320 197 7 57 359 247 554 3.49e-40 149
Msa1132640 MtrunA17_Chr6g0460141 50.000 132 63 1 99 230 16 144 3.54e-40 139
Msa1132640 MtrunA17_Chr7g0269541 28.358 335 202 7 51 359 226 548 4.28e-40 149
Msa1132640 MtrunA17_Chr3g0129921 30.794 315 182 7 66 356 251 553 7.72e-40 148
Msa1132640 MtrunA17_Chr7g0233801 29.524 315 196 6 60 359 242 545 1.11e-39 147
Msa1132640 MtrunA17_Chr1g0195351 31.847 314 179 9 65 359 248 545 1.50e-39 147
Msa1132640 MtrunA17_Chr1g0147051 29.290 338 199 8 50 359 274 599 4.24e-39 146
Msa1132640 MtrunA17_Chr7g0233831 29.430 316 196 6 60 359 243 547 4.75e-39 145
Msa1132640 MtrunA17_Chr1g0192311 29.483 329 180 7 60 359 257 562 6.69e-38 143
Msa1132640 MtrunA17_Chr1g0192281 29.483 329 180 7 60 359 257 562 6.69e-38 143
Msa1132640 MtrunA17_Chr3g0080381 30.218 321 195 7 57 360 313 621 7.26e-38 143
Msa1132640 MtrunA17_Chr1g0192321 29.841 315 195 6 60 359 272 575 7.28e-38 143
Msa1132640 MtrunA17_Chr5g0437831 30.625 320 188 9 48 348 65 369 7.64e-38 139
Msa1132640 MtrunA17_Chr7g0233771 30.420 286 175 5 60 332 242 516 9.28e-38 142
Msa1132640 MtrunA17_Chr2g0295531 32.042 284 170 5 60 332 232 503 1.35e-37 142
Msa1132640 MtrunA17_Chr4g0069861 30.094 319 194 7 53 356 202 506 1.62e-37 141
Msa1132640 MtrunA17_Chr8g0354911 30.503 318 192 7 60 360 300 605 1.81e-37 142
Msa1132640 MtrunA17_Chr8g0391291 30.745 322 191 8 60 361 228 537 3.44e-37 140
Msa1132640 MtrunA17_Chr1g0192301 29.518 332 176 8 60 359 262 567 3.55e-37 141
Msa1132640 MtrunA17_Chr3g0126771 29.747 316 194 7 60 359 286 589 6.06e-37 140
Msa1132640 MtrunA17_Chr2g0302001 32.906 234 137 5 140 359 202 429 1.65e-36 137
Msa1132640 MtrunA17_Chr4g0000121 28.875 329 182 7 60 359 244 549 2.48e-36 138
Msa1132640 MtrunA17_Chr8g0391311 30.745 322 191 8 60 361 230 539 2.59e-36 138
Msa1132640 MtrunA17_Chr7g0270691 29.102 323 201 6 54 359 230 541 4.21e-36 137
Msa1132640 MtrunA17_Chr4g0011751 29.022 317 195 8 61 359 177 481 2.56e-35 134
Msa1132640 MtrunA17_Chr4g0011791 30.128 312 191 8 60 359 205 501 2.59e-35 135
Msa1132640 MtrunA17_Chr1g0195311 32.342 269 152 7 107 359 52 306 7.68e-35 130
Msa1132640 MtrunA17_Chr7g0268241 30.421 309 175 8 68 360 59 343 1.08e-34 130
Msa1132640 MtrunA17_Chr2g0334111 28.889 315 189 7 57 359 24 315 1.24e-34 129
Msa1132640 MtrunA17_Chr1g0194091 29.642 307 184 7 54 348 249 535 1.76e-34 133
Msa1132640 MtrunA17_Chr5g0440901 46.324 136 70 1 176 308 13 148 1.76e-34 124
Msa1132640 MtrunA17_Chr4g0067611 29.577 284 177 5 60 332 258 529 6.57e-34 132
Msa1132640 MtrunA17_Chr4g0069891 30.717 293 177 6 52 332 279 557 1.20e-33 131
Msa1132640 MtrunA17_Chr7g0272151 28.125 320 195 7 60 359 271 575 4.25e-33 129
Msa1132640 MtrunA17_Chr7g0234711 32.287 223 131 5 152 359 44 261 5.25e-33 124
Msa1132640 MtrunA17_Chr4g0067601 29.965 287 176 6 57 332 257 529 1.66e-32 127
Msa1132640 MtrunA17_Chr2g0295551 31.183 279 169 5 65 332 255 521 4.02e-32 126
Msa1132640 MtrunA17_Chr2g0301931 28.873 284 174 6 85 352 12 283 4.71e-32 122
Msa1132640 MtrunA17_Chr8g0345211 27.848 316 203 6 53 356 269 571 4.29e-31 124
Msa1132640 MtrunA17_Chr1g0194081 28.797 316 198 7 57 359 254 555 5.02e-31 123
Msa1132640 MtrunA17_Chr7g0270631 30.345 290 166 7 65 332 800 1075 4.46e-30 121
Msa1132640 MtrunA17_Chr7g0270631 29.286 280 174 5 65 332 252 519 2.27e-28 116
Msa1132640 MtrunA17_Chr8g0380581 25.737 373 234 11 15 359 11 368 9.79e-30 117
Msa1132640 MtrunA17_Chr7g0270651 29.861 288 176 7 57 332 258 531 1.20e-29 119
Msa1132640 MtrunA17_Chr7g0270661 29.966 297 181 8 49 332 249 531 1.40e-29 119
Msa1132640 MtrunA17_Chr7g0270671 29.966 297 181 8 49 332 249 531 1.40e-29 119
Msa1132640 MtrunA17_Chr2g0295541 28.213 319 173 6 56 332 248 552 7.15e-28 114
Msa1132640 MtrunA17_Chr5g0440891 38.202 178 89 3 56 230 41 200 1.90e-26 104
Msa1132640 MtrunA17_Chr7g0271381 26.606 327 195 10 60 359 240 548 6.98e-26 108
Msa1132640 MtrunA17_Chr6g0459791 36.752 117 74 0 241 357 1 117 5.51e-21 87.4
Msa1132640 MtrunA17_Chr4g0073911 32.086 187 109 4 71 250 19 194 4.43e-20 88.2
Msa1132640 MtrunA17_Chr5g0440881 30.303 132 88 2 228 356 9 139 2.29e-16 75.5
Msa1132640 MtrunA17_Chr2g0299431 26.293 232 139 6 51 265 34 250 8.34e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1132640 AT5G47500.1 70.637 361 85 2 9 361 15 362 0.0 545
Msa1132640 AT5G19730.1 51.903 289 138 1 68 356 94 381 1.13e-104 313
Msa1132640 AT2G36710.1 48.534 307 146 3 59 360 90 389 4.40e-96 292
Msa1132640 AT5G55590.1 49.834 301 147 3 58 356 80 378 6.78e-95 288
Msa1132640 AT1G05310.1 44.660 309 156 4 60 360 91 392 5.31e-87 268
Msa1132640 AT2G36700.1 45.000 300 156 4 59 356 41 333 7.83e-87 266
Msa1132640 AT5G55590.2 53.012 249 114 2 58 304 80 327 6.84e-86 263
Msa1132640 AT5G07430.1 42.053 302 165 4 58 358 65 357 8.46e-85 261
Msa1132640 AT2G21610.1 42.715 302 154 6 59 358 50 334 2.55e-81 252
Msa1132640 AT2G21610.2 42.715 302 154 6 59 358 55 339 3.75e-81 252
Msa1132640 AT1G69940.1 41.639 305 168 4 58 361 65 360 8.70e-80 248
Msa1132640 AT5G07420.1 41.639 305 168 4 58 361 65 360 3.46e-79 247
Msa1132640 AT5G07410.1 41.311 305 169 4 58 361 65 360 5.39e-79 246
Msa1132640 AT5G61680.1 41.060 302 169 3 58 358 67 360 3.67e-77 242
Msa1132640 AT3G17060.1 36.441 354 200 6 8 356 3 336 5.63e-74 233
Msa1132640 AT3G29090.1 40.836 311 164 7 58 357 5 306 3.44e-70 222
Msa1132640 AT5G26810.1 32.877 365 197 8 10 357 1 334 3.74e-60 197
Msa1132640 AT3G24130.1 35.294 306 178 4 60 356 34 328 6.03e-60 196
Msa1132640 AT2G47280.1 37.785 307 169 6 60 357 35 328 2.41e-59 195
Msa1132640 AT5G18990.1 34.314 306 181 4 60 356 29 323 1.94e-57 190
Msa1132640 AT3G05620.1 35.974 303 177 6 62 359 243 533 7.48e-53 183
Msa1132640 AT2G19150.1 35.294 306 181 4 58 357 39 333 2.24e-50 172
Msa1132640 AT5G09760.1 34.277 318 175 7 60 355 241 546 1.98e-49 174
Msa1132640 AT5G09760.2 34.277 318 175 7 60 355 267 572 2.52e-49 174
Msa1132640 AT3G05610.1 33.762 311 180 6 60 356 256 554 1.31e-48 174
Msa1132640 AT3G06830.1 29.474 380 227 10 8 359 194 560 4.03e-48 171
Msa1132640 AT5G27870.1 32.154 311 185 6 60 356 253 551 1.13e-45 166
Msa1132640 AT2G19150.2 38.645 251 138 3 58 302 39 279 3.00e-45 157
Msa1132640 AT5G49180.1 33.016 315 182 7 60 358 260 561 4.31e-45 163
Msa1132640 AT5G64640.1 31.790 324 186 8 60 359 289 601 5.44e-45 163
Msa1132640 AT4G02330.1 31.429 315 187 7 59 356 258 560 5.01e-44 160
Msa1132640 AT5G20860.2 33.123 317 179 10 60 359 230 530 9.94e-44 159
Msa1132640 AT2G26440.1 30.530 321 195 7 53 359 233 539 1.62e-43 158
Msa1132640 AT3G59010.1 33.007 306 181 8 62 359 231 520 5.22e-43 156
Msa1132640 AT3G43270.1 31.138 334 193 7 51 359 196 517 1.07e-41 153
Msa1132640 AT1G11590.1 30.573 314 191 7 60 359 216 516 2.88e-41 152
Msa1132640 AT3G27980.1 30.573 314 191 7 60 359 189 489 3.90e-41 151
Msa1132640 AT4G33220.2 29.129 333 200 7 51 359 74 394 4.38e-41 149
Msa1132640 AT4G02300.1 31.761 318 190 7 57 359 219 524 6.33e-41 151
Msa1132640 AT3G10720.2 30.519 308 185 7 70 360 313 608 8.22e-41 152
Msa1132640 AT1G02810.1 31.834 289 170 6 59 332 264 540 1.57e-40 150
Msa1132640 AT1G11580.1 29.206 315 196 6 60 359 247 549 1.82e-40 150
Msa1132640 AT1G11580.2 29.206 315 196 6 60 359 268 570 1.94e-40 150
Msa1132640 AT4G33220.1 29.129 333 200 7 51 359 195 515 2.29e-40 149
Msa1132640 AT3G14310.1 32.698 315 183 7 61 359 283 584 3.40e-40 150
Msa1132640 AT4G00190.1 30.938 320 194 9 55 359 159 466 3.96e-40 148
Msa1132640 AT1G23200.1 31.715 309 189 8 60 359 251 546 4.17e-40 149
Msa1132640 AT1G53840.1 29.677 310 197 5 60 359 281 579 8.20e-40 149
Msa1132640 AT3G10710.1 30.323 310 194 6 60 359 256 553 1.79e-39 147
Msa1132640 AT5G04970.1 31.068 309 182 8 70 360 318 613 6.11e-39 146
Msa1132640 AT1G53830.1 31.847 314 187 7 61 359 278 579 6.28e-39 146
Msa1132640 AT4G15980.1 33.439 314 184 8 60 359 392 694 7.58e-39 147
Msa1132640 AT4G15980.2 33.439 314 184 8 60 359 402 704 1.03e-38 147
Msa1132640 AT1G11370.1 29.323 266 163 5 46 299 34 286 2.38e-38 139
Msa1132640 AT2G47550.1 31.447 318 189 8 59 359 245 550 6.70e-38 143
Msa1132640 AT4G33230.1 28.857 350 217 9 26 356 259 595 4.72e-37 141
Msa1132640 AT5G04960.1 28.939 311 198 6 60 359 258 556 4.72e-37 140
Msa1132640 AT4G03930.1 28.981 314 196 7 60 359 216 516 5.60e-37 140
Msa1132640 AT5G51490.1 28.571 378 235 10 3 359 165 528 1.56e-36 139
Msa1132640 AT2G45220.1 30.599 317 188 10 54 356 203 501 2.17e-36 138
Msa1132640 AT5G53370.2 30.063 316 193 7 60 359 310 613 3.27e-36 139
Msa1132640 AT2G47030.1 32.867 286 163 6 65 334 282 554 3.79e-36 138
Msa1132640 AT5G53370.1 30.063 316 193 7 60 359 274 577 3.80e-36 138
Msa1132640 AT2G26450.1 27.476 313 201 6 61 359 303 603 1.30e-35 137
Msa1132640 AT3G49220.3 31.329 316 189 8 60 359 330 633 1.53e-35 137
Msa1132640 AT3G49220.1 31.329 316 189 8 60 359 285 588 1.66e-35 137
Msa1132640 AT4G02320.1 27.928 333 211 7 44 359 190 510 2.81e-35 135
Msa1132640 AT2G43050.1 30.072 276 175 5 90 359 245 508 3.43e-35 135
Msa1132640 AT3G14300.1 29.290 338 204 10 41 356 634 958 4.04e-35 137
Msa1132640 AT3G47400.1 30.133 375 215 12 14 359 230 586 8.44e-35 135
Msa1132640 AT3G10720.1 32.340 235 139 5 140 360 24 252 1.24e-34 128
Msa1132640 AT2G47040.1 32.155 283 169 8 65 334 289 561 2.13e-34 134
Msa1132640 AT3G60730.1 31.104 299 179 8 76 359 225 511 7.75e-34 131
Msa1132640 AT5G20860.1 33.333 249 137 8 113 344 251 487 2.91e-32 127
Msa1132640 AT3G62170.1 31.119 286 168 7 65 334 282 554 1.55e-31 125
Msa1132640 AT5G51500.1 27.979 386 223 13 3 356 167 529 1.01e-30 122
Msa1132640 AT1G44980.1 24.405 168 114 3 68 234 86 241 4.63e-11 62.8

Find 126 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTTATTAAAATATTTA+TGG 0.116018 7_3:+85524194 None:intergenic
ACATTCATTGTATTGTTATC+TGG 0.184382 7_3:+85523829 None:intergenic
ACATTCTTTGCAATTGCTTT+TGG 0.241254 7_3:-85524049 Msa1132640:CDS
ACGAAGCTCTTCCTGATAAA+TGG 0.247766 7_3:+85521166 None:intergenic
TTTATAGTAAGAGGTATATT+TGG 0.263269 7_3:+85524285 None:intergenic
CACACGGTGGTTGGGATGAT+TGG 0.283922 7_3:-85521413 Msa1132640:CDS
TTAATTAGGACTGTGTTCTT+TGG 0.291942 7_3:-85521271 Msa1132640:intron
TGTATTTATACTATATATCT+TGG 0.300937 7_3:+85524168 None:intergenic
GTTATCTGGGACGGAATTTA+CGG 0.302481 7_3:+85523843 None:intergenic
GCGGTGGCGTTTCGCATATC+AGG 0.303188 7_3:-85522939 Msa1132640:CDS
GTGAAGCCTGTCTTCTCGTC+TGG 0.316654 7_3:+85521543 None:intergenic
AAACAGCACATCCATTTATC+AGG 0.324261 7_3:-85521177 Msa1132640:CDS
GTCAAGAATAATGCTATATA+TGG 0.331491 7_3:+85521106 None:intergenic
ACAAATAAGAATAGAATGTT+TGG 0.342302 7_3:+85524118 None:intergenic
CAATTCTCAACTCTCTATAA+TGG 0.365946 7_3:-85524088 Msa1132640:exon
ATCCAGCACTGATTTGAATA+AGG 0.375627 7_3:+85523806 None:intergenic
AGGTTCGATCCATTGGTTCT+TGG 0.385472 7_3:+85523930 None:intergenic
CATTCATTGTATTGTTATCT+GGG 0.392536 7_3:+85523830 None:intergenic
TATACTAATATTTACCTTGA+AGG 0.396670 7_3:+85523383 None:intergenic
TAATAAAGAAATGCTTATAA+AGG 0.398499 7_3:-85520887 Msa1132640:three_prime_UTR
CATTTCTTTATTACAATTGA+AGG 0.404631 7_3:+85520897 None:intergenic
TGCAAAGAATATCACCTTCA+AGG 0.406243 7_3:-85523397 Msa1132640:intron
CGTCTTGGACGGACTGGAAC+TGG 0.408345 7_3:+85523866 None:intergenic
CTTTGGAGTGTACAAATGTT+GGG 0.416963 7_3:-85521254 Msa1132640:CDS
TGTAGTAGACAATGTATTGA+AGG 0.418098 7_3:+85523960 None:intergenic
TGCATCCCGGGCATTGGTGC+CGG 0.422340 7_3:+85522972 None:intergenic
CTATATGTAGGACGTGCAAT+GGG 0.422683 7_3:-85521486 Msa1132640:CDS
CGATGCAAGGTGACAGGCTC+AGG 0.427837 7_3:-85521513 Msa1132640:CDS
TTGCCTATCCTGTGCTGCTA+TGG 0.436377 7_3:+85521569 None:intergenic
GTATTTATACTATATATCTT+GGG 0.437627 7_3:+85524169 None:intergenic
ACAGCGCCGGCACCAATGCC+CGG 0.437990 7_3:-85522978 Msa1132640:CDS
TACCGACAGGTTCGATCCAT+TGG 0.440708 7_3:+85523923 None:intergenic
ATTCTTTGCAGAGAAATAGT+TGG 0.443410 7_3:+85523410 None:intergenic
AATCCTTGCATCCCGGGCAT+TGG 0.445967 7_3:+85522966 None:intergenic
CGCTTGCAATCCTTGCATCC+CGG 0.449510 7_3:+85522959 None:intergenic
GAGAAATAACAGTGATTGAA+TGG 0.452312 7_3:-85523509 Msa1132640:CDS
TCAGGAAGAGCTTCGTCAAT+GGG 0.453223 7_3:-85521159 Msa1132640:CDS
TCCCCTTGAAATGTTATGTA+CGG 0.454195 7_3:+85523537 None:intergenic
TGCTGGTAGGCATTACTTCA+AGG 0.455937 7_3:-85522856 Msa1132640:CDS
GTCCTTATTCAAATCAGTGC+TGG 0.456134 7_3:-85523808 Msa1132640:CDS
TTCTCTGGCTGTGGCTTCCA+CGG 0.457319 7_3:-85522903 Msa1132640:CDS
GGGGACAAAGCATACTTCTC+TGG 0.459293 7_3:-85522918 Msa1132640:CDS
TACTATATATCTTGGGAGCA+TGG 0.461957 7_3:+85524176 None:intergenic
ACACGGTGGTTGGGATGATT+GGG 0.462473 7_3:-85521412 Msa1132640:CDS
AGGCAGTACGTGGTGTGTCA+TGG 0.467502 7_3:-85521219 Msa1132640:CDS
ACTTGCATGTTGTTAAATGC+AGG 0.468024 7_3:-85524021 Msa1132640:CDS
AAGCCGTACATAACATTTCA+AGG 0.471438 7_3:-85523540 Msa1132640:CDS
GTGTACAAATGTTGGGGACC+AGG 0.473026 7_3:-85521247 Msa1132640:CDS
GCTTGCAATCCTTGCATCCC+GGG 0.474029 7_3:+85522960 None:intergenic
CCACGTACTGCCTCTGCTCC+TGG 0.474972 7_3:+85521229 None:intergenic
CATTCAATAGCAACAAGGTT+CGG 0.477326 7_3:-85521594 Msa1132640:CDS
CATGATAGAGCTTGTGATCC+TGG 0.477605 7_3:-85523486 Msa1132640:CDS
TACAATGTGTGTAAGTAAAG+AGG 0.481245 7_3:+85524234 None:intergenic
GTACGTAACTGTTGTCCGTT+GGG 0.482645 7_3:+85523462 None:intergenic
CATTGTATTGTTATCTGGGA+CGG 0.482901 7_3:+85523834 None:intergenic
TTCAAGGAATGCTACATTGA+GGG 0.487345 7_3:-85522840 Msa1132640:CDS
GTAATCACAGTTGATATTAA+CGG 0.490404 7_3:-85523895 Msa1132640:CDS
ATTGCACGTCCTACATATAG+TGG 0.491228 7_3:+85521489 None:intergenic
TGAAGGTAGTGTGGTGATGA+TGG 0.492113 7_3:+85523977 None:intergenic
TTACGGCGTCTTGGACGGAC+TGG 0.502096 7_3:+85523860 None:intergenic
TCTTTGGAGTGTACAAATGT+TGG 0.504995 7_3:-85521255 Msa1132640:CDS
GGACGGAATTTACGGCGTCT+TGG 0.507752 7_3:+85523851 None:intergenic
ATCAGGAAGAGCTTCGTCAA+TGG 0.508046 7_3:-85521160 Msa1132640:CDS
GGAATTTACGGCGTCTTGGA+CGG 0.512824 7_3:+85523855 None:intergenic
TACATAACATTTCAAGGGGA+AGG 0.513154 7_3:-85523534 Msa1132640:CDS
AACTGTTGTCCGTTGGGACC+AGG 0.513658 7_3:+85523468 None:intergenic
TATAGTTGCACACGGTGGTT+GGG 0.519022 7_3:-85521421 Msa1132640:CDS
GTTATGTACGGCTTTGTCAC+GGG 0.520170 7_3:+85523549 None:intergenic
ATATAGTTGCACACGGTGGT+TGG 0.521044 7_3:-85521422 Msa1132640:CDS
GCACCAATGCCCGGGATGCA+AGG 0.523172 7_3:-85522969 Msa1132640:CDS
TTCGTACGATGCAAGGTGAC+AGG 0.524057 7_3:-85521519 Msa1132640:CDS
CAGCGCCGGCACCAATGCCC+GGG 0.525664 7_3:-85522977 Msa1132640:CDS
GGCAGTACGTGGTGTGTCAT+GGG 0.529160 7_3:-85521218 Msa1132640:CDS
TGAAGCCTGTCTTCTCGTCT+GGG 0.532273 7_3:+85521544 None:intergenic
ATTACAATTGAAGGAGGTCA+AGG 0.532533 7_3:+85520906 None:intergenic
TGTGTCATGGGCACGAGAGT+TGG 0.536786 7_3:-85521206 Msa1132640:CDS
ACTATATGTAGGACGTGCAA+TGG 0.542419 7_3:-85521487 Msa1132640:CDS
GTTCGGATCCATAGCAGCAC+AGG 0.542754 7_3:-85521577 Msa1132640:CDS
GTGATTACACGGTTACCGAC+AGG 0.543167 7_3:+85523910 None:intergenic
CGGTGGCGTTTCGCATATCA+GGG 0.544327 7_3:-85522938 Msa1132640:CDS
AAAGCATACTTCTCTGGCTG+TGG 0.552260 7_3:-85522912 Msa1132640:CDS
GATCCATAGCAGCACAGGAT+AGG 0.554332 7_3:-85521572 Msa1132640:CDS
TGTTATGTACGGCTTTGTCA+CGG 0.554628 7_3:+85523548 None:intergenic
CAATACCCAGACGAGAAGAC+AGG 0.556786 7_3:-85521549 Msa1132640:CDS
GCTTCGTCAATGGGAGACAT+TGG 0.566621 7_3:-85521150 Msa1132640:CDS
AACTTGATGGTGTAGAGAGA+AGG 0.569823 7_3:-85523580 Msa1132640:intron
TTTGGAGTGTACAAATGTTG+GGG 0.570214 7_3:-85521253 Msa1132640:CDS
AGTACGTAACTGTTGTCCGT+TGG 0.570235 7_3:+85523461 None:intergenic
CTTCAAGGAATGCTACATTG+AGG 0.571058 7_3:-85522841 Msa1132640:CDS
GACACACTTTGTGACGATGC+TGG 0.571568 7_3:-85522873 Msa1132640:CDS
CAAACTCATCCAAGAACCAA+TGG 0.573843 7_3:-85523939 Msa1132640:CDS
ATCATACCTTGTACATGGAT+CGG 0.576866 7_3:+85522790 None:intergenic
ACAGTTGATATTAACGGTGG+AGG 0.587474 7_3:-85523889 Msa1132640:CDS
TTCTTTATTACAATTGAAGG+AGG 0.588089 7_3:+85520900 None:intergenic
CTTCACATTCGTACGATGCA+AGG 0.588588 7_3:-85521526 Msa1132640:CDS
AATGAATCATACCTTGTACA+TGG 0.588920 7_3:+85522785 None:intergenic
GAGACATTGGATCGCACCTA+CGG 0.590531 7_3:-85521137 Msa1132640:CDS
AGCCGTACATAACATTTCAA+GGG 0.591308 7_3:-85523539 Msa1132640:CDS
ACATAACATTTCAAGGGGAA+GGG 0.591823 7_3:-85523533 Msa1132640:CDS
TGCAAAGAATGTAAAAGAGT+AGG 0.602823 7_3:+85524060 None:intergenic
TAATATCAACTGTGATTACA+CGG 0.603000 7_3:+85523899 None:intergenic
ATGTTGGGGACCAGGAGCAG+AGG 0.612194 7_3:-85521239 Msa1132640:CDS
GCTTGTGATCCTGGTCCCAA+CGG 0.619512 7_3:-85523477 Msa1132640:CDS
AAATGGCCGATCCATGTACA+AGG 0.619865 7_3:-85522796 Msa1132640:intron
TTGATGGTGTAGAGAGAAGG+TGG 0.630741 7_3:-85523577 Msa1132640:intron
CACTTTGTGACGATGCTGGT+AGG 0.638179 7_3:-85522869 Msa1132640:CDS
GCCGTACATAACATTTCAAG+GGG 0.638381 7_3:-85523538 Msa1132640:CDS
ATATATGGCTAAGCATCCGT+AGG 0.641001 7_3:+85521121 None:intergenic
GAGCATGTAGAATACAGCGC+CGG 0.642880 7_3:-85522991 Msa1132640:intron
AAAGAGGAAGAACAAATGAG+AGG 0.645359 7_3:+85524250 None:intergenic
CCAGGAGCAGAGGCAGTACG+TGG 0.646245 7_3:-85521229 Msa1132640:CDS
ACAATGTATTGAAGGTAGTG+TGG 0.646502 7_3:+85523968 None:intergenic
CGGGATGCAAGGATTGCAAG+CGG 0.655175 7_3:-85522958 Msa1132640:CDS
AACCAATGGATCGAACCTGT+CGG 0.668325 7_3:-85523925 Msa1132640:CDS
GATGCAAGGATTGCAAGCGG+TGG 0.671605 7_3:-85522955 Msa1132640:CDS
GGCTCAGGTCCACTATATGT+AGG 0.671680 7_3:-85521498 Msa1132640:CDS
ATCACAGTTGATATTAACGG+TGG 0.676561 7_3:-85523892 Msa1132640:CDS
AGCTGCATTCAATAGCAACA+AGG 0.679636 7_3:-85521599 Msa1132640:CDS
TTTGATGATATAGTTGCACA+CGG 0.696006 7_3:-85521429 Msa1132640:CDS
ACAACAGTCTCTCCGCAGAG+AGG 0.708082 7_3:+85520963 None:intergenic
TAATATAGTTATCCTCTCTG+CGG 0.714189 7_3:-85520975 Msa1132640:three_prime_UTR
GGTGGCGTTTCGCATATCAG+GGG 0.723699 7_3:-85522937 Msa1132640:CDS
TATATGTAGGACGTGCAATG+GGG 0.736057 7_3:-85521485 Msa1132640:CDS
ATGGTGTAGAGAGAAGGTGG+TGG 0.744314 7_3:-85523574 Msa1132640:intron
AAAGTGTGTCTTGTGCACCG+TGG 0.789181 7_3:+85522886 None:intergenic
GATGATATAGTTGCACACGG+TGG 0.832583 7_3:-85521426 Msa1132640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTAAAAAAATAATAAAA+AGG + chr7_3:85522796-85522815 None:intergenic 0.0%
!! TATATTTACAATAACAATAT+TGG + chr7_3:85521896-85521915 None:intergenic 10.0%
!!! TTTTAATAATAGTACTTAAT+AGG - chr7_3:85522807-85522826 Msa1132640:CDS 10.0%
!! CATGGTTATTAAAATATTTA+TGG + chr7_3:85520952-85520971 None:intergenic 15.0%
!! GTATTTATACTATATATCTT+GGG + chr7_3:85520977-85520996 None:intergenic 15.0%
!! TGTATTTATACTATATATCT+TGG + chr7_3:85520978-85520997 None:intergenic 15.0%
!! GTATTAATTCTTAATATTGT+TGG - chr7_3:85521486-85521505 Msa1132640:CDS 15.0%
!! TTTGAATGAAAGTAATAATT+TGG - chr7_3:85521529-85521548 Msa1132640:CDS 15.0%
!! TAGTTTATTTATCTCAATTA+TGG - chr7_3:85522407-85522426 Msa1132640:intron 15.0%
!!! ATGATTATTTTATTGAAAAG+TGG - chr7_3:85522927-85522946 Msa1132640:CDS 15.0%
!! AAACTAACTAATAGTTAATA+AGG - chr7_3:85523261-85523280 Msa1132640:intron 15.0%
!! AATAGTTAATAAGGATAATT+TGG - chr7_3:85523270-85523289 Msa1132640:intron 15.0%
!! AATAATTAAAAGAAAGAGAA+TGG + chr7_3:85523304-85523323 None:intergenic 15.0%
!! TAATAAAAACATCTCTTAAT+TGG - chr7_3:85523504-85523523 Msa1132640:CDS 15.0%
!! TAATAAAGAAATGCTTATAA+AGG - chr7_3:85524256-85524275 Msa1132640:five_prime_UTR 15.0%
!!! TTTATAGTAAGAGGTATATT+TGG + chr7_3:85520861-85520880 None:intergenic 20.0%
!!! TTTGTGTATTTTATAGTAAG+AGG + chr7_3:85520870-85520889 None:intergenic 20.0%
!! ACAAATAAGAATAGAATGTT+TGG + chr7_3:85521028-85521047 None:intergenic 20.0%
!! CTTAATTAATGCTAAAGAAA+AGG + chr7_3:85521436-85521455 None:intergenic 20.0%
!! TATACTAATATTTACCTTGA+AGG + chr7_3:85521763-85521782 None:intergenic 20.0%
!! TTTACAATAACAATATTGGT+AGG + chr7_3:85521892-85521911 None:intergenic 20.0%
!! TAAGCTAAAGAAGTTAAAAA+AGG + chr7_3:85522057-85522076 None:intergenic 20.0%
!! ATTTCCAAAAATGAAATCAA+TGG + chr7_3:85522331-85522350 None:intergenic 20.0%
!!! ATTGATTTCATTTTTGGAAA+TGG - chr7_3:85522330-85522349 Msa1132640:intron 20.0%
!! TAACATCAAAATTCATTAAG+TGG - chr7_3:85522522-85522541 Msa1132640:intron 20.0%
!! TCCAATGTAGAAAAAAATTA+GGG + chr7_3:85522974-85522993 None:intergenic 20.0%
!! TGTTCAACAAAAAAAAAAAC+AGG + chr7_3:85523073-85523092 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + chr7_3:85523184-85523203 None:intergenic 20.0%
!! ATTTGTGGATAAACTTAATT+AGG - chr7_3:85523858-85523877 Msa1132640:CDS 20.0%
!!! ATTTTTGTTCTTTTCAACAT+AGG - chr7_3:85524131-85524150 Msa1132640:five_prime_UTR 20.0%
!! CATTTCTTTATTACAATTGA+AGG + chr7_3:85524249-85524268 None:intergenic 20.0%
! TAATATCAACTGTGATTACA+CGG + chr7_3:85521247-85521266 None:intergenic 25.0%
!! GTAATCACAGTTGATATTAA+CGG - chr7_3:85521248-85521267 Msa1132640:CDS 25.0%
! CATTCATTGTATTGTTATCT+GGG + chr7_3:85521316-85521335 None:intergenic 25.0%
! ACATTCATTGTATTGTTATC+TGG + chr7_3:85521317-85521336 None:intergenic 25.0%
! GAATGAAAGTAATAATTTGG+TGG - chr7_3:85521532-85521551 Msa1132640:CDS 25.0%
! TAACTGATAATTAACCTCAA+TGG - chr7_3:85521821-85521840 Msa1132640:intron 25.0%
! AACTGATAATTAACCTCAAT+GGG - chr7_3:85521822-85521841 Msa1132640:intron 25.0%
!! TTTTACCTAAGTTAAAAAGC+TGG + chr7_3:85522080-85522099 None:intergenic 25.0%
!! TATTTTATTGAAAAGTGGTG+TGG - chr7_3:85522932-85522951 Msa1132640:CDS 25.0%
!! ATTTTATTGAAAAGTGGTGT+GGG - chr7_3:85522933-85522952 Msa1132640:CDS 25.0%
! CCAATGTAGAAAAAAATTAG+GGG + chr7_3:85522973-85522992 None:intergenic 25.0%
! CTCCAATGTAGAAAAAAATT+AGG + chr7_3:85522975-85522994 None:intergenic 25.0%
!!! CTAATTTTTTTCTACATTGG+AGG - chr7_3:85522973-85522992 Msa1132640:CDS 25.0%
!!! GAGATGTTTTTATTAAGAGA+TGG + chr7_3:85523499-85523518 None:intergenic 25.0%
! AAACGATAAGAAGGATAAAA+AGG + chr7_3:85523783-85523802 None:intergenic 25.0%
!! TTTTATCCTTCTTATCGTTT+TGG - chr7_3:85523783-85523802 Msa1132640:intron 25.0%
! TAAAATTCAGAAAATCAGTG+TGG + chr7_3:85523826-85523845 None:intergenic 25.0%
! GTCAAGAATAATGCTATATA+TGG + chr7_3:85524040-85524059 None:intergenic 25.0%
!! TTCCTTTAAAAATGTGAGTA+GGG + chr7_3:85524065-85524084 None:intergenic 25.0%
!! TTTCCTTTAAAAATGTGAGT+AGG + chr7_3:85524066-85524085 None:intergenic 25.0%
!! ATATGACAACTCTTTTGTAT+GGG + chr7_3:85524113-85524132 None:intergenic 25.0%
!! AATATGACAACTCTTTTGTA+TGG + chr7_3:85524114-85524133 None:intergenic 25.0%
! TTCTTTATTACAATTGAAGG+AGG + chr7_3:85524246-85524265 None:intergenic 25.0%
TACAATGTGTGTAAGTAAAG+AGG + chr7_3:85520912-85520931 None:intergenic 30.0%
CAATTCTCAACTCTCTATAA+TGG - chr7_3:85521055-85521074 Msa1132640:three_prime_UTR 30.0%
TGCAAAGAATGTAAAAGAGT+AGG + chr7_3:85521086-85521105 None:intergenic 30.0%
! ACATTCTTTGCAATTGCTTT+TGG - chr7_3:85521094-85521113 Msa1132640:three_prime_UTR 30.0%
TGTAGTAGACAATGTATTGA+AGG + chr7_3:85521186-85521205 None:intergenic 30.0%
! TAACTTGCACCATTATTTAG+TGG - chr7_3:85521399-85521418 Msa1132640:CDS 30.0%
CATGTAACACCACTAAATAA+TGG + chr7_3:85521411-85521430 None:intergenic 30.0%
GAGAAATAACAGTGATTGAA+TGG - chr7_3:85521634-85521653 Msa1132640:intron 30.0%
ATTCTTTGCAGAGAAATAGT+TGG + chr7_3:85521736-85521755 None:intergenic 30.0%
CTTTCATTTCAATGTAAGAG+TGG - chr7_3:85522007-85522026 Msa1132640:intron 30.0%
AATGAATCATACCTTGTACA+TGG + chr7_3:85522361-85522380 None:intergenic 30.0%
TGGCAACATTACCTTAATAA+TGG - chr7_3:85522427-85522446 Msa1132640:intron 30.0%
GGCAACATTACCTTAATAAT+GGG - chr7_3:85522428-85522447 Msa1132640:intron 30.0%
CTTTAACGAACCCATTATTA+AGG + chr7_3:85522441-85522460 None:intergenic 30.0%
AGTGTGACAGAGAAATAAAA+AGG + chr7_3:85522487-85522506 None:intergenic 30.0%
AAAACATTGAGATGCGTAAA+GGG - chr7_3:85522550-85522569 Msa1132640:intron 30.0%
TTGCCAAGTGTTTATGAATA+TGG - chr7_3:85522588-85522607 Msa1132640:intron 30.0%
AGTCCATATTCATAAACACT+TGG + chr7_3:85522594-85522613 None:intergenic 30.0%
ACATATATTAGTTCAATGCC+TGG + chr7_3:85522690-85522709 None:intergenic 30.0%
! TTTTGATAGATGATCTCCAT+TGG + chr7_3:85522845-85522864 None:intergenic 30.0%
AATGGAGATCATCTATCAAA+AGG - chr7_3:85522844-85522863 Msa1132640:CDS 30.0%
! TTTTATTGAAAAGTGGTGTG+GGG - chr7_3:85522934-85522953 Msa1132640:CDS 30.0%
!! AAAATTAGGGGTATCTTAAG+TGG + chr7_3:85522961-85522980 None:intergenic 30.0%
!!! CCCCTAATTTTTTTCTACAT+TGG - chr7_3:85522970-85522989 Msa1132640:CDS 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG + chr7_3:85523352-85523371 None:intergenic 30.0%
!! AAACGACACTCATTTTGAAA+CGG - chr7_3:85523353-85523372 Msa1132640:intron 30.0%
!! ATGGAGTAGTTTGACTTTTA+GGG + chr7_3:85523480-85523499 None:intergenic 30.0%
! TTTGATGATATAGTTGCACA+CGG - chr7_3:85523714-85523733 Msa1132640:intron 30.0%
!! TTAACTGTCTTGTTGATTTG+TGG - chr7_3:85523843-85523862 Msa1132640:CDS 30.0%
TTAATTAGGACTGTGTTCTT+TGG - chr7_3:85523872-85523891 Msa1132640:CDS 30.0%
! TTCCCTACTCACATTTTTAA+AGG - chr7_3:85524060-85524079 Msa1132640:CDS 30.0%
TAATATAGTTATCCTCTCTG+CGG - chr7_3:85524168-85524187 Msa1132640:five_prime_UTR 30.0%
AAAGAGGAAGAACAAATGAG+AGG + chr7_3:85520896-85520915 None:intergenic 35.0%
TACTATATATCTTGGGAGCA+TGG + chr7_3:85520970-85520989 None:intergenic 35.0%
ACTTGCATGTTGTTAAATGC+AGG - chr7_3:85521122-85521141 Msa1132640:exon 35.0%
ACAATGTATTGAAGGTAGTG+TGG + chr7_3:85521178-85521197 None:intergenic 35.0%
! ATCACAGTTGATATTAACGG+TGG - chr7_3:85521251-85521270 Msa1132640:CDS 35.0%
CATTGTATTGTTATCTGGGA+CGG + chr7_3:85521312-85521331 None:intergenic 35.0%
!! ATCCAGCACTGATTTGAATA+AGG + chr7_3:85521340-85521359 None:intergenic 35.0%
AAGCCGTACATAACATTTCA+AGG - chr7_3:85521603-85521622 Msa1132640:CDS 35.0%
AGCCGTACATAACATTTCAA+GGG - chr7_3:85521604-85521623 Msa1132640:CDS 35.0%
TCCCCTTGAAATGTTATGTA+CGG + chr7_3:85521609-85521628 None:intergenic 35.0%
TACATAACATTTCAAGGGGA+AGG - chr7_3:85521609-85521628 Msa1132640:CDS 35.0%
ACATAACATTTCAAGGGGAA+GGG - chr7_3:85521610-85521629 Msa1132640:intron 35.0%
TGCAAAGAATATCACCTTCA+AGG - chr7_3:85521746-85521765 Msa1132640:intron 35.0%
ATTAACCTCAATGGGAACAA+AGG - chr7_3:85521830-85521849 Msa1132640:intron 35.0%
TTAACCTCAATGGGAACAAA+GGG - chr7_3:85521831-85521850 Msa1132640:intron 35.0%
!!! GCTTACCAGCTTTTTAACTT+AGG - chr7_3:85522072-85522091 Msa1132640:intron 35.0%
TTCAAGGAATGCTACATTGA+GGG - chr7_3:85522303-85522322 Msa1132640:intron 35.0%
!!! GGCTCCATTGATTTCATTTT+TGG - chr7_3:85522324-85522343 Msa1132640:intron 35.0%
ATCATACCTTGTACATGGAT+CGG + chr7_3:85522356-85522375 None:intergenic 35.0%
! CTTTACGCATCTCAATGTTT+TGG + chr7_3:85522551-85522570 None:intergenic 35.0%
CAAAACATTGAGATGCGTAA+AGG - chr7_3:85522549-85522568 Msa1132640:intron 35.0%
! GGATGCTCTAATGTTCTTTT+GGG + chr7_3:85522721-85522740 None:intergenic 35.0%
! TGGATGCTCTAATGTTCTTT+TGG + chr7_3:85522722-85522741 None:intergenic 35.0%
! CTCACATTTGAGGTCTTAAA+CGG - chr7_3:85522751-85522770 Msa1132640:intron 35.0%
! TCACATTTGAGGTCTTAAAC+GGG - chr7_3:85522752-85522771 Msa1132640:intron 35.0%
AAAAGGTGATGTGTCTATGT+GGG + chr7_3:85522779-85522798 None:intergenic 35.0%
AAAAAGGTGATGTGTCTATG+TGG + chr7_3:85522780-85522799 None:intergenic 35.0%
! ACTCTTTACCATTTAAGACC+CGG + chr7_3:85523012-85523031 None:intergenic 35.0%
!!! GACACTCATTTTGAAACGAA+GGG + chr7_3:85523114-85523133 None:intergenic 35.0%
! TGTGCAGAGTATTAATTGGA+GGG + chr7_3:85523218-85523237 None:intergenic 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG + chr7_3:85523222-85523241 None:intergenic 35.0%
!! GATGGAGTAGTTTGACTTTT+AGG + chr7_3:85523481-85523500 None:intergenic 35.0%
TCTCTTAATTGGAGATTCTC+AGG - chr7_3:85523515-85523534 Msa1132640:CDS 35.0%
CATTCAATAGCAACAAGGTT+CGG - chr7_3:85523549-85523568 Msa1132640:CDS 35.0%
!!! ACTTGTTTTTGTTGTTAGCG+TGG + chr7_3:85523758-85523777 None:intergenic 35.0%
TCATCACCAAAACGATAAGA+AGG + chr7_3:85523792-85523811 None:intergenic 35.0%
!! TCTTTGGAGTGTACAAATGT+TGG - chr7_3:85523888-85523907 Msa1132640:CDS 35.0%
!! CTTTGGAGTGTACAAATGTT+GGG - chr7_3:85523889-85523908 Msa1132640:CDS 35.0%
! TTTGGAGTGTACAAATGTTG+GGG - chr7_3:85523890-85523909 Msa1132640:CDS 35.0%
AAACAGCACATCCATTTATC+AGG - chr7_3:85523966-85523985 Msa1132640:CDS 35.0%
ATTACAATTGAAGGAGGTCA+AGG + chr7_3:85524240-85524259 None:intergenic 35.0%
CAAACTCATCCAAGAACCAA+TGG - chr7_3:85521204-85521223 Msa1132640:CDS 40.0%
! ACAGTTGATATTAACGGTGG+AGG - chr7_3:85521254-85521273 Msa1132640:CDS 40.0%
! GTTATCTGGGACGGAATTTA+CGG + chr7_3:85521303-85521322 None:intergenic 40.0%
GTCCTTATTCAAATCAGTGC+TGG - chr7_3:85521335-85521354 Msa1132640:intron 40.0%
! AGTGCTGGATTCTACAAGTA+TGG - chr7_3:85521350-85521369 Msa1132640:intron 40.0%
GGTGGTGCATATAAACTTGA+TGG - chr7_3:85521550-85521569 Msa1132640:CDS 40.0%
!! AACTTGATGGTGTAGAGAGA+AGG - chr7_3:85521563-85521582 Msa1132640:CDS 40.0%
! TGTTATGTACGGCTTTGTCA+CGG + chr7_3:85521598-85521617 None:intergenic 40.0%
GCCGTACATAACATTTCAAG+GGG - chr7_3:85521605-85521624 Msa1132640:CDS 40.0%
CTTCAAGGAATGCTACATTG+AGG - chr7_3:85522302-85522321 Msa1132640:intron 40.0%
TGCGTAAAGGGATACTAGTA+AGG - chr7_3:85522562-85522581 Msa1132640:intron 40.0%
! CTACCACGTTTTTAATGACC+AGG - chr7_3:85522669-85522688 Msa1132640:intron 40.0%
ATGCCTGGTCATTAAAAACG+TGG + chr7_3:85522675-85522694 None:intergenic 40.0%
TCAAATGTGAGTCTCGATTG+TGG + chr7_3:85522742-85522761 None:intergenic 40.0%
ACAATCGAGACTCACATTTG+AGG - chr7_3:85522741-85522760 Msa1132640:intron 40.0%
ATAGATGATCTCCATTGGAG+AGG + chr7_3:85522840-85522859 None:intergenic 40.0%
!! CTACATTGGAGGTACCTATT+AGG - chr7_3:85522984-85523003 Msa1132640:CDS 40.0%
! TTTAAGACCCGGTACCTAAT+AGG + chr7_3:85523001-85523020 None:intergenic 40.0%
!! ATTAGGTACCGGGTCTTAAA+TGG - chr7_3:85523001-85523020 Msa1132640:intron 40.0%
GGGTCTTAAATGGTAAAGAG+TGG - chr7_3:85523011-85523030 Msa1132640:intron 40.0%
!!! CGACACTCATTTTGAAACGA+AGG + chr7_3:85523115-85523134 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG + chr7_3:85523219-85523238 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr7_3:85523357-85523376 Msa1132640:intron 40.0%
AGCTGCATTCAATAGCAACA+AGG - chr7_3:85523544-85523563 Msa1132640:CDS 40.0%
ATTGCACGTCCTACATATAG+TGG + chr7_3:85523657-85523676 None:intergenic 40.0%
ACTATATGTAGGACGTGCAA+TGG - chr7_3:85523656-85523675 Msa1132640:intron 40.0%
CTATATGTAGGACGTGCAAT+GGG - chr7_3:85523657-85523676 Msa1132640:intron 40.0%
TATATGTAGGACGTGCAATG+GGG - chr7_3:85523658-85523677 Msa1132640:intron 40.0%
ACGAAGCTCTTCCTGATAAA+TGG + chr7_3:85523980-85523999 None:intergenic 40.0%
ATATATGGCTAAGCATCCGT+AGG + chr7_3:85524025-85524044 None:intergenic 40.0%
! TGAAGGTAGTGTGGTGATGA+TGG + chr7_3:85521169-85521188 None:intergenic 45.0%
!! AGGTTCGATCCATTGGTTCT+TGG + chr7_3:85521216-85521235 None:intergenic 45.0%
AACCAATGGATCGAACCTGT+CGG - chr7_3:85521218-85521237 Msa1132640:CDS 45.0%
!! TTGATGGTGTAGAGAGAAGG+TGG - chr7_3:85521566-85521585 Msa1132640:CDS 45.0%
! GTTATGTACGGCTTTGTCAC+GGG + chr7_3:85521597-85521616 None:intergenic 45.0%
CATGATAGAGCTTGTGATCC+TGG - chr7_3:85521657-85521676 Msa1132640:intron 45.0%
GTACGTAACTGTTGTCCGTT+GGG + chr7_3:85521684-85521703 None:intergenic 45.0%
AGTACGTAACTGTTGTCCGT+TGG + chr7_3:85521685-85521704 None:intergenic 45.0%
AAAGCATACTTCTCTGGCTG+TGG - chr7_3:85522231-85522250 Msa1132640:intron 45.0%
TGCTGGTAGGCATTACTTCA+AGG - chr7_3:85522287-85522306 Msa1132640:intron 45.0%
AAATGGCCGATCCATGTACA+AGG - chr7_3:85522347-85522366 Msa1132640:intron 45.0%
TAGGTACTCAACCTCTCCAA+TGG - chr7_3:85522826-85522845 Msa1132640:CDS 45.0%
!! TGGAGGTACCTATTAGGTAC+CGG - chr7_3:85522990-85523009 Msa1132640:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr7_3:85523356-85523375 Msa1132640:intron 45.0%
!!! TCATTTTGAAACGGAGGGAG+CGG - chr7_3:85523362-85523381 Msa1132640:intron 45.0%
CTTCACATTCGTACGATGCA+AGG - chr7_3:85523617-85523636 Msa1132640:intron 45.0%
GATGATATAGTTGCACACGG+TGG - chr7_3:85523717-85523736 Msa1132640:intron 45.0%
ATATAGTTGCACACGGTGGT+TGG - chr7_3:85523721-85523740 Msa1132640:intron 45.0%
TATAGTTGCACACGGTGGTT+GGG - chr7_3:85523722-85523741 Msa1132640:intron 45.0%
ATCAGGAAGAGCTTCGTCAA+TGG - chr7_3:85523983-85524002 Msa1132640:CDS 45.0%
TCAGGAAGAGCTTCGTCAAT+GGG - chr7_3:85523984-85524003 Msa1132640:CDS 45.0%
TACCGACAGGTTCGATCCAT+TGG + chr7_3:85521223-85521242 None:intergenic 50.0%
GTGATTACACGGTTACCGAC+AGG + chr7_3:85521236-85521255 None:intergenic 50.0%
GGAATTTACGGCGTCTTGGA+CGG + chr7_3:85521291-85521310 None:intergenic 50.0%
ATGGTGTAGAGAGAAGGTGG+TGG - chr7_3:85521569-85521588 Msa1132640:CDS 50.0%
ACCTCAATGGGAACAAAGGG+AGG - chr7_3:85521834-85521853 Msa1132640:intron 50.0%
TCCTCCCTTTGTTCCCATTG+AGG + chr7_3:85521838-85521857 None:intergenic 50.0%
CAATGGGAACAAAGGGAGGA+AGG - chr7_3:85521838-85521857 Msa1132640:intron 50.0%
GAGCATGTAGAATACAGCGC+CGG - chr7_3:85522152-85522171 Msa1132640:intron 50.0%
GGGGACAAAGCATACTTCTC+TGG - chr7_3:85522225-85522244 Msa1132640:intron 50.0%
AAAGTGTGTCTTGTGCACCG+TGG + chr7_3:85522260-85522279 None:intergenic 50.0%
GACACACTTTGTGACGATGC+TGG - chr7_3:85522270-85522289 Msa1132640:intron 50.0%
CACTTTGTGACGATGCTGGT+AGG - chr7_3:85522274-85522293 Msa1132640:intron 50.0%
!! GGAGGTACCTATTAGGTACC+GGG - chr7_3:85522991-85523010 Msa1132640:intron 50.0%
GATCCATAGCAGCACAGGAT+AGG - chr7_3:85523571-85523590 Msa1132640:intron 50.0%
! TTGCCTATCCTGTGCTGCTA+TGG + chr7_3:85523577-85523596 None:intergenic 50.0%
CAATACCCAGACGAGAAGAC+AGG - chr7_3:85523594-85523613 Msa1132640:intron 50.0%
TGAAGCCTGTCTTCTCGTCT+GGG + chr7_3:85523602-85523621 None:intergenic 50.0%
TTCGTACGATGCAAGGTGAC+AGG - chr7_3:85523624-85523643 Msa1132640:intron 50.0%
GGCTCAGGTCCACTATATGT+AGG - chr7_3:85523645-85523664 Msa1132640:intron 50.0%
ACACGGTGGTTGGGATGATT+GGG - chr7_3:85523731-85523750 Msa1132640:intron 50.0%
GTGTACAAATGTTGGGGACC+AGG - chr7_3:85523896-85523915 Msa1132640:CDS 50.0%
GCTTCGTCAATGGGAGACAT+TGG - chr7_3:85523993-85524012 Msa1132640:CDS 50.0%
GAGACATTGGATCGCACCTA+CGG - chr7_3:85524006-85524025 Msa1132640:CDS 50.0%
GGACGGAATTTACGGCGTCT+TGG + chr7_3:85521295-85521314 None:intergenic 55.0%
GCTTGTGATCCTGGTCCCAA+CGG - chr7_3:85521666-85521685 Msa1132640:intron 55.0%
AACTGTTGTCCGTTGGGACC+AGG + chr7_3:85521678-85521697 None:intergenic 55.0%
AATCCTTGCATCCCGGGCAT+TGG + chr7_3:85522180-85522199 None:intergenic 55.0%
GCTTGCAATCCTTGCATCCC+GGG + chr7_3:85522186-85522205 None:intergenic 55.0%
CGCTTGCAATCCTTGCATCC+CGG + chr7_3:85522187-85522206 None:intergenic 55.0%
CGGGATGCAAGGATTGCAAG+CGG - chr7_3:85522185-85522204 Msa1132640:intron 55.0%
GATGCAAGGATTGCAAGCGG+TGG - chr7_3:85522188-85522207 Msa1132640:intron 55.0%
CGGTGGCGTTTCGCATATCA+GGG - chr7_3:85522205-85522224 Msa1132640:intron 55.0%
GGTGGCGTTTCGCATATCAG+GGG - chr7_3:85522206-85522225 Msa1132640:intron 55.0%
TTCTCTGGCTGTGGCTTCCA+CGG - chr7_3:85522240-85522259 Msa1132640:intron 55.0%
GTTCGGATCCATAGCAGCAC+AGG - chr7_3:85523566-85523585 Msa1132640:CDS 55.0%
GTGAAGCCTGTCTTCTCGTC+TGG + chr7_3:85523603-85523622 None:intergenic 55.0%
CACACGGTGGTTGGGATGAT+TGG - chr7_3:85523730-85523749 Msa1132640:intron 55.0%
AGGCAGTACGTGGTGTGTCA+TGG - chr7_3:85523924-85523943 Msa1132640:CDS 55.0%
GGCAGTACGTGGTGTGTCAT+GGG - chr7_3:85523925-85523944 Msa1132640:CDS 55.0%
TGTGTCATGGGCACGAGAGT+TGG - chr7_3:85523937-85523956 Msa1132640:CDS 55.0%
ACAACAGTCTCTCCGCAGAG+AGG + chr7_3:85524183-85524202 None:intergenic 55.0%
!! CGTCTTGGACGGACTGGAAC+TGG + chr7_3:85521280-85521299 None:intergenic 60.0%
TTACGGCGTCTTGGACGGAC+TGG + chr7_3:85521286-85521305 None:intergenic 60.0%
GCGGTGGCGTTTCGCATATC+AGG - chr7_3:85522204-85522223 Msa1132640:intron 60.0%
CGATGCAAGGTGACAGGCTC+AGG - chr7_3:85523630-85523649 Msa1132640:intron 60.0%
ATGTTGGGGACCAGGAGCAG+AGG - chr7_3:85523904-85523923 Msa1132640:CDS 60.0%
!! TGCATCCCGGGCATTGGTGC+CGG + chr7_3:85522174-85522193 None:intergenic 65.0%
GCACCAATGCCCGGGATGCA+AGG - chr7_3:85522174-85522193 Msa1132640:intron 65.0%
CCACGTACTGCCTCTGCTCC+TGG + chr7_3:85523917-85523936 None:intergenic 65.0%
CCAGGAGCAGAGGCAGTACG+TGG - chr7_3:85523914-85523933 Msa1132640:CDS 65.0%
!! ACAGCGCCGGCACCAATGCC+CGG - chr7_3:85522165-85522184 Msa1132640:intron 70.0%
!! CAGCGCCGGCACCAATGCCC+GGG - chr7_3:85522166-85522185 Msa1132640:intron 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 85520857 85524308 85520857 ID=Msa1132640;Name=Msa1132640
chr7_3 mRNA 85520857 85524308 85520857 ID=Msa1132640-mRNA-1;Parent=Msa1132640;Name=Msa1132640-mRNA-1;_AED=0.04;_eAED=0.04;_QI=217|1|1|1|1|1|5|272|361
chr7_3 exon 85520857 85521285 85520857 ID=Msa1132640-mRNA-1:exon:25661;Parent=Msa1132640-mRNA-1
chr7_3 exon 85521390 85521628 85521390 ID=Msa1132640-mRNA-1:exon:25660;Parent=Msa1132640-mRNA-1
chr7_3 exon 85522797 85523003 85522797 ID=Msa1132640-mRNA-1:exon:25659;Parent=Msa1132640-mRNA-1
chr7_3 exon 85523398 85523587 85523398 ID=Msa1132640-mRNA-1:exon:25658;Parent=Msa1132640-mRNA-1
chr7_3 exon 85523799 85524308 85523799 ID=Msa1132640-mRNA-1:exon:25657;Parent=Msa1132640-mRNA-1
chr7_3 five_prime_UTR 85524092 85524308 85524092 ID=Msa1132640-mRNA-1:five_prime_utr;Parent=Msa1132640-mRNA-1
chr7_3 CDS 85523799 85524091 85523799 ID=Msa1132640-mRNA-1:cds;Parent=Msa1132640-mRNA-1
chr7_3 CDS 85523398 85523587 85523398 ID=Msa1132640-mRNA-1:cds;Parent=Msa1132640-mRNA-1
chr7_3 CDS 85522797 85523003 85522797 ID=Msa1132640-mRNA-1:cds;Parent=Msa1132640-mRNA-1
chr7_3 CDS 85521390 85521628 85521390 ID=Msa1132640-mRNA-1:cds;Parent=Msa1132640-mRNA-1
chr7_3 CDS 85521129 85521285 85521129 ID=Msa1132640-mRNA-1:cds;Parent=Msa1132640-mRNA-1
chr7_3 three_prime_UTR 85520857 85521128 85520857 ID=Msa1132640-mRNA-1:three_prime_utr;Parent=Msa1132640-mRNA-1
Gene Sequence

>Msa1132640

ATGGATACACCTACTCTTTTACATTCTTTGCAATTGCTTTTGGTTTTCACTTGCATGTTGTTAAATGCAGGAATAGTGACACTAGCTGCACGCCATCATCACCACACTACCTTCAATACATTGTCTACTACAAACTCATCCAAGAACCAATGGATCGAACCTGTCGGTAACCGTGTAATCACAGTTGATATTAACGGTGGAGGCCAGTTCCAGTCCGTCCAAGACGCCGTAAATTCCGTCCCAGATAACAATACAATGAATGTCCTTATTCAAATCAGTGCTGGATTCTACAAAGAGAAGGTGGTGGTTCCCGTGACAAAGCCGTACATAACATTTCAAGGGGAAGGGAGAGAAATAACAGTGATTGAATGGCATGATAGAGCTTGTGATCCTGGTCCCAACGGACAACAGTTACGTACTTATAGAACTGCTTCTGTTACTGTCTTTGCCAACTATTTCTCTGCAAAGAATATCACCTTCAAGAATACAGCGCCGGCACCAATGCCCGGGATGCAAGGATTGCAAGCGGTGGCGTTTCGCATATCAGGGGACAAAGCATACTTCTCTGGCTGTGGCTTCCACGGTGCACAAGACACACTTTGTGACGATGCTGGTAGGCATTACTTCAAGGAATGCTACATTGAGGGCTCCATTGATTTCATTTTTGGAAATGGCCGATCCATGTACAAGGATTGTGAGCTGCATTCAATAGCAACAAGGTTCGGATCCATAGCAGCACAGGATAGGCAATACCCAGACGAGAAGACAGGCTTCACATTCGTACGATGCAAGGTGACAGGCTCAGGTCCACTATATGTAGGACGTGCAATGGGGCAATACTCAAGAATAGTTTATGCATATACATACTTTGATGATATAGTTGCACACGGTGGTTGGGATGATTGGGACCACGCTAACAACAAAAACAAGACTGTGTTCTTTGGAGTGTACAAATGTTGGGGACCAGGAGCAGAGGCAGTACGTGGTGTGTCATGGGCACGAGAGTTGGATTTCGAAACAGCACATCCATTTATCAGGAAGAGCTTCGTCAATGGGAGACATTGGATCGCACCTACGGATGCTTAG
Protein sequence

>Msa1132640

MDTPTLLHSLQLLLVFTCMLLNAGIVTLAARHHHHTTFNTLSTTNSSKNQWIEPVGNRVITVDINGGGQFQSVQDAVNSVPDNNTMNVLIQISAGFYKEKVVVPVTKPYITFQGEGREITVIEWHDRACDPGPNGQQLRTYRTASVTVFANYFSAKNITFKNTAPAPMPGMQGLQAVAFRISGDKAYFSGCGFHGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRQYPDEKTGFTFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDIVAHGGWDDWDHANNKNKTVFFGVYKCWGPGAEAVRGVSWARELDFETAHPFIRKSFVNGRHWIAPTDA*