AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0833700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833700 MtrunA17_Chr5g0399221 97.802 364 5 1 1 361 1 364 0.0 743
Msa0833700 MtrunA17_Chr6g0487101 52.941 289 135 1 68 356 93 380 8.82e-108 320
Msa0833700 MtrunA17_Chr8g0389771 53.265 291 133 2 68 356 88 377 6.18e-106 316
Msa0833700 MtrunA17_Chr7g0258341 47.869 305 144 4 60 356 90 387 7.53e-93 283
Msa0833700 MtrunA17_Chr5g0446301 46.599 294 151 4 66 356 67 357 5.75e-92 279
Msa0833700 MtrunA17_Chr7g0265201 44.575 341 163 8 25 356 62 385 1.53e-87 269
Msa0833700 MtrunA17_Chr1g0194311 39.335 361 201 6 12 356 10 368 5.86e-84 259
Msa0833700 MtrunA17_Chr4g0070051 42.857 308 155 7 58 358 52 345 1.18e-75 237
Msa0833700 MtrunA17_Chr2g0294321 40.656 305 165 4 57 356 53 346 1.01e-70 224
Msa0833700 MtrunA17_Chr7g0217941 40.323 310 165 7 59 357 5 305 2.48e-70 222
Msa0833700 MtrunA17_Chr6g0466961 36.741 313 179 6 50 356 25 324 3.52e-68 217
Msa0833700 MtrunA17_Chr6g0453551 38.835 309 171 6 59 357 5 305 1.09e-67 216
Msa0833700 MtrunA17_Chr6g0466951 37.700 313 176 6 50 356 54 353 4.70e-66 213
Msa0833700 MtrunA17_Chr4g0037751 33.894 357 218 6 12 358 15 363 2.00e-65 211
Msa0833700 MtrunA17_Chr5g0420831 34.746 354 215 5 13 358 18 363 2.18e-65 211
Msa0833700 MtrunA17_Chr1g0161731 35.632 348 201 7 16 356 8 339 5.82e-64 207
Msa0833700 MtrunA17_Chr2g0295851 39.016 305 161 5 57 356 52 336 9.33e-64 206
Msa0833700 MtrunA17_Chr8g0351871 37.013 308 171 6 58 357 31 323 1.62e-62 202
Msa0833700 MtrunA17_Chr5g0440871 35.065 308 174 6 56 357 32 319 1.93e-52 176
Msa0833700 MtrunA17_Chr7g0272141 30.769 364 222 7 14 359 183 534 1.04e-47 169
Msa0833700 MtrunA17_Chr4g0069881 31.447 318 192 6 57 359 221 527 5.05e-47 167
Msa0833700 MtrunA17_Chr6g0459781 44.828 174 93 2 59 232 12 182 6.24e-47 158
Msa0833700 MtrunA17_Chr8g0345281 32.063 315 187 6 60 359 279 581 3.96e-46 166
Msa0833700 MtrunA17_Chr8g0384121 32.476 311 177 7 68 356 254 553 4.11e-46 165
Msa0833700 MtrunA17_Chr1g0192331 31.366 322 184 9 60 359 214 520 1.54e-44 160
Msa0833700 MtrunA17_Chr1g0195301 32.484 314 179 8 65 359 248 547 7.90e-43 156
Msa0833700 MtrunA17_Chr1g0195321 32.484 314 179 8 65 359 248 547 7.90e-43 156
Msa0833700 MtrunA17_Chr4g0069841 30.032 313 192 6 62 359 208 508 8.69e-43 155
Msa0833700 MtrunA17_Chr2g0314691 39.000 200 119 3 161 358 5 203 9.28e-43 147
Msa0833700 MtrunA17_Chr1g0175661 31.132 318 190 7 59 359 245 550 1.34e-42 155
Msa0833700 MtrunA17_Chr4g0011771 31.790 324 191 8 50 359 198 505 3.37e-42 154
Msa0833700 MtrunA17_Chr4g0025841 36.000 250 138 4 58 304 35 265 4.17e-42 148
Msa0833700 MtrunA17_Chr4g0018371 32.595 316 186 9 60 359 227 531 4.45e-42 154
Msa0833700 MtrunA17_Chr7g0269531 31.546 317 185 8 59 356 251 554 5.61e-42 154
Msa0833700 MtrunA17_Chr7g0265161 36.486 222 115 5 140 357 14 213 9.75e-42 146
Msa0833700 MtrunA17_Chr1g0195331 32.166 314 180 8 65 359 248 547 1.23e-41 153
Msa0833700 MtrunA17_Chr3g0090091 30.380 316 193 8 60 359 216 520 2.01e-41 152
Msa0833700 MtrunA17_Chr3g0080391 30.769 312 194 6 60 361 269 568 6.39e-41 151
Msa0833700 MtrunA17_Chr3g0079341 31.469 286 172 7 60 332 215 489 7.58e-41 150
Msa0833700 MtrunA17_Chr3g0079361 30.063 316 194 8 60 359 215 519 9.19e-41 150
Msa0833700 MtrunA17_Chr3g0079351 29.747 316 195 8 60 359 215 519 9.67e-41 150
Msa0833700 MtrunA17_Chr7g0269511 29.787 329 201 8 49 359 240 556 1.65e-40 150
Msa0833700 MtrunA17_Chr8g0354901 30.449 312 195 6 60 361 276 575 2.89e-40 149
Msa0833700 MtrunA17_Chr2g0302021 29.560 318 197 6 57 359 212 517 3.43e-40 148
Msa0833700 MtrunA17_Chr7g0269521 29.375 320 197 7 57 359 247 554 3.49e-40 149
Msa0833700 MtrunA17_Chr6g0460141 50.000 132 63 1 99 230 16 144 3.54e-40 139
Msa0833700 MtrunA17_Chr7g0269541 28.358 335 202 7 51 359 226 548 4.28e-40 149
Msa0833700 MtrunA17_Chr3g0129921 30.794 315 182 7 66 356 251 553 7.72e-40 148
Msa0833700 MtrunA17_Chr7g0233801 29.524 315 196 6 60 359 242 545 1.11e-39 147
Msa0833700 MtrunA17_Chr1g0195351 31.847 314 179 9 65 359 248 545 1.50e-39 147
Msa0833700 MtrunA17_Chr1g0147051 29.290 338 199 8 50 359 274 599 4.24e-39 146
Msa0833700 MtrunA17_Chr7g0233831 29.430 316 196 6 60 359 243 547 4.75e-39 145
Msa0833700 MtrunA17_Chr1g0192311 29.483 329 180 7 60 359 257 562 6.69e-38 143
Msa0833700 MtrunA17_Chr1g0192281 29.483 329 180 7 60 359 257 562 6.69e-38 143
Msa0833700 MtrunA17_Chr3g0080381 30.218 321 195 7 57 360 313 621 7.26e-38 143
Msa0833700 MtrunA17_Chr1g0192321 29.841 315 195 6 60 359 272 575 7.28e-38 143
Msa0833700 MtrunA17_Chr5g0437831 30.625 320 188 9 48 348 65 369 7.64e-38 139
Msa0833700 MtrunA17_Chr7g0233771 30.420 286 175 5 60 332 242 516 9.28e-38 142
Msa0833700 MtrunA17_Chr2g0295531 32.042 284 170 5 60 332 232 503 1.35e-37 142
Msa0833700 MtrunA17_Chr4g0069861 30.094 319 194 7 53 356 202 506 1.62e-37 141
Msa0833700 MtrunA17_Chr8g0354911 30.503 318 192 7 60 360 300 605 1.81e-37 142
Msa0833700 MtrunA17_Chr8g0391291 30.745 322 191 8 60 361 228 537 3.44e-37 140
Msa0833700 MtrunA17_Chr1g0192301 29.518 332 176 8 60 359 262 567 3.55e-37 141
Msa0833700 MtrunA17_Chr3g0126771 29.747 316 194 7 60 359 286 589 6.06e-37 140
Msa0833700 MtrunA17_Chr2g0302001 32.906 234 137 5 140 359 202 429 1.65e-36 137
Msa0833700 MtrunA17_Chr4g0000121 28.875 329 182 7 60 359 244 549 2.48e-36 138
Msa0833700 MtrunA17_Chr8g0391311 30.745 322 191 8 60 361 230 539 2.59e-36 138
Msa0833700 MtrunA17_Chr7g0270691 29.102 323 201 6 54 359 230 541 4.21e-36 137
Msa0833700 MtrunA17_Chr4g0011751 29.022 317 195 8 61 359 177 481 2.56e-35 134
Msa0833700 MtrunA17_Chr4g0011791 30.128 312 191 8 60 359 205 501 2.59e-35 135
Msa0833700 MtrunA17_Chr1g0195311 32.342 269 152 7 107 359 52 306 7.68e-35 130
Msa0833700 MtrunA17_Chr7g0268241 30.421 309 175 8 68 360 59 343 1.08e-34 130
Msa0833700 MtrunA17_Chr2g0334111 28.889 315 189 7 57 359 24 315 1.24e-34 129
Msa0833700 MtrunA17_Chr1g0194091 29.642 307 184 7 54 348 249 535 1.76e-34 133
Msa0833700 MtrunA17_Chr5g0440901 46.324 136 70 1 176 308 13 148 1.76e-34 124
Msa0833700 MtrunA17_Chr4g0067611 29.577 284 177 5 60 332 258 529 6.57e-34 132
Msa0833700 MtrunA17_Chr4g0069891 30.717 293 177 6 52 332 279 557 1.20e-33 131
Msa0833700 MtrunA17_Chr7g0272151 28.125 320 195 7 60 359 271 575 4.25e-33 129
Msa0833700 MtrunA17_Chr7g0234711 32.287 223 131 5 152 359 44 261 5.25e-33 124
Msa0833700 MtrunA17_Chr4g0067601 29.965 287 176 6 57 332 257 529 1.66e-32 127
Msa0833700 MtrunA17_Chr2g0295551 31.183 279 169 5 65 332 255 521 4.02e-32 126
Msa0833700 MtrunA17_Chr2g0301931 28.873 284 174 6 85 352 12 283 4.71e-32 122
Msa0833700 MtrunA17_Chr8g0345211 27.848 316 203 6 53 356 269 571 4.29e-31 124
Msa0833700 MtrunA17_Chr1g0194081 28.797 316 198 7 57 359 254 555 5.02e-31 123
Msa0833700 MtrunA17_Chr7g0270631 30.345 290 166 7 65 332 800 1075 4.46e-30 121
Msa0833700 MtrunA17_Chr7g0270631 29.286 280 174 5 65 332 252 519 2.27e-28 116
Msa0833700 MtrunA17_Chr8g0380581 25.737 373 234 11 15 359 11 368 9.79e-30 117
Msa0833700 MtrunA17_Chr7g0270651 29.861 288 176 7 57 332 258 531 1.20e-29 119
Msa0833700 MtrunA17_Chr7g0270661 29.966 297 181 8 49 332 249 531 1.40e-29 119
Msa0833700 MtrunA17_Chr7g0270671 29.966 297 181 8 49 332 249 531 1.40e-29 119
Msa0833700 MtrunA17_Chr2g0295541 28.213 319 173 6 56 332 248 552 7.15e-28 114
Msa0833700 MtrunA17_Chr5g0440891 38.202 178 89 3 56 230 41 200 1.90e-26 104
Msa0833700 MtrunA17_Chr7g0271381 26.606 327 195 10 60 359 240 548 6.98e-26 108
Msa0833700 MtrunA17_Chr6g0459791 36.752 117 74 0 241 357 1 117 5.51e-21 87.4
Msa0833700 MtrunA17_Chr4g0073911 32.086 187 109 4 71 250 19 194 4.43e-20 88.2
Msa0833700 MtrunA17_Chr5g0440881 30.303 132 88 2 228 356 9 139 2.29e-16 75.5
Msa0833700 MtrunA17_Chr2g0299431 26.293 232 139 6 51 265 34 250 8.34e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833700 AT5G47500.1 70.637 361 85 2 9 361 15 362 0.0 545
Msa0833700 AT5G19730.1 51.903 289 138 1 68 356 94 381 1.13e-104 313
Msa0833700 AT2G36710.1 48.534 307 146 3 59 360 90 389 4.40e-96 292
Msa0833700 AT5G55590.1 49.834 301 147 3 58 356 80 378 6.78e-95 288
Msa0833700 AT1G05310.1 44.660 309 156 4 60 360 91 392 5.31e-87 268
Msa0833700 AT2G36700.1 45.000 300 156 4 59 356 41 333 7.83e-87 266
Msa0833700 AT5G55590.2 53.012 249 114 2 58 304 80 327 6.84e-86 263
Msa0833700 AT5G07430.1 42.053 302 165 4 58 358 65 357 8.46e-85 261
Msa0833700 AT2G21610.1 42.715 302 154 6 59 358 50 334 2.55e-81 252
Msa0833700 AT2G21610.2 42.715 302 154 6 59 358 55 339 3.75e-81 252
Msa0833700 AT1G69940.1 41.639 305 168 4 58 361 65 360 8.70e-80 248
Msa0833700 AT5G07420.1 41.639 305 168 4 58 361 65 360 3.46e-79 247
Msa0833700 AT5G07410.1 41.311 305 169 4 58 361 65 360 5.39e-79 246
Msa0833700 AT5G61680.1 41.060 302 169 3 58 358 67 360 3.67e-77 242
Msa0833700 AT3G17060.1 36.441 354 200 6 8 356 3 336 5.63e-74 233
Msa0833700 AT3G29090.1 40.836 311 164 7 58 357 5 306 3.44e-70 222
Msa0833700 AT5G26810.1 32.877 365 197 8 10 357 1 334 3.74e-60 197
Msa0833700 AT3G24130.1 35.294 306 178 4 60 356 34 328 6.03e-60 196
Msa0833700 AT2G47280.1 37.785 307 169 6 60 357 35 328 2.41e-59 195
Msa0833700 AT5G18990.1 34.314 306 181 4 60 356 29 323 1.94e-57 190
Msa0833700 AT3G05620.1 35.974 303 177 6 62 359 243 533 7.48e-53 183
Msa0833700 AT2G19150.1 35.294 306 181 4 58 357 39 333 2.24e-50 172
Msa0833700 AT5G09760.1 34.277 318 175 7 60 355 241 546 1.98e-49 174
Msa0833700 AT5G09760.2 34.277 318 175 7 60 355 267 572 2.52e-49 174
Msa0833700 AT3G05610.1 33.762 311 180 6 60 356 256 554 1.31e-48 174
Msa0833700 AT3G06830.1 29.474 380 227 10 8 359 194 560 4.03e-48 171
Msa0833700 AT5G27870.1 32.154 311 185 6 60 356 253 551 1.13e-45 166
Msa0833700 AT2G19150.2 38.645 251 138 3 58 302 39 279 3.00e-45 157
Msa0833700 AT5G49180.1 33.016 315 182 7 60 358 260 561 4.31e-45 163
Msa0833700 AT5G64640.1 31.790 324 186 8 60 359 289 601 5.44e-45 163
Msa0833700 AT4G02330.1 31.429 315 187 7 59 356 258 560 5.01e-44 160
Msa0833700 AT5G20860.2 33.123 317 179 10 60 359 230 530 9.94e-44 159
Msa0833700 AT2G26440.1 30.530 321 195 7 53 359 233 539 1.62e-43 158
Msa0833700 AT3G59010.1 33.007 306 181 8 62 359 231 520 5.22e-43 156
Msa0833700 AT3G43270.1 31.138 334 193 7 51 359 196 517 1.07e-41 153
Msa0833700 AT1G11590.1 30.573 314 191 7 60 359 216 516 2.88e-41 152
Msa0833700 AT3G27980.1 30.573 314 191 7 60 359 189 489 3.90e-41 151
Msa0833700 AT4G33220.2 29.129 333 200 7 51 359 74 394 4.38e-41 149
Msa0833700 AT4G02300.1 31.761 318 190 7 57 359 219 524 6.33e-41 151
Msa0833700 AT3G10720.2 30.519 308 185 7 70 360 313 608 8.22e-41 152
Msa0833700 AT1G02810.1 31.834 289 170 6 59 332 264 540 1.57e-40 150
Msa0833700 AT1G11580.1 29.206 315 196 6 60 359 247 549 1.82e-40 150
Msa0833700 AT1G11580.2 29.206 315 196 6 60 359 268 570 1.94e-40 150
Msa0833700 AT4G33220.1 29.129 333 200 7 51 359 195 515 2.29e-40 149
Msa0833700 AT3G14310.1 32.698 315 183 7 61 359 283 584 3.40e-40 150
Msa0833700 AT4G00190.1 30.938 320 194 9 55 359 159 466 3.96e-40 148
Msa0833700 AT1G23200.1 31.715 309 189 8 60 359 251 546 4.17e-40 149
Msa0833700 AT1G53840.1 29.677 310 197 5 60 359 281 579 8.20e-40 149
Msa0833700 AT3G10710.1 30.323 310 194 6 60 359 256 553 1.79e-39 147
Msa0833700 AT5G04970.1 31.068 309 182 8 70 360 318 613 6.11e-39 146
Msa0833700 AT1G53830.1 31.847 314 187 7 61 359 278 579 6.28e-39 146
Msa0833700 AT4G15980.1 33.439 314 184 8 60 359 392 694 7.58e-39 147
Msa0833700 AT4G15980.2 33.439 314 184 8 60 359 402 704 1.03e-38 147
Msa0833700 AT1G11370.1 29.323 266 163 5 46 299 34 286 2.38e-38 139
Msa0833700 AT2G47550.1 31.447 318 189 8 59 359 245 550 6.70e-38 143
Msa0833700 AT4G33230.1 28.857 350 217 9 26 356 259 595 4.72e-37 141
Msa0833700 AT5G04960.1 28.939 311 198 6 60 359 258 556 4.72e-37 140
Msa0833700 AT4G03930.1 28.981 314 196 7 60 359 216 516 5.60e-37 140
Msa0833700 AT5G51490.1 28.571 378 235 10 3 359 165 528 1.56e-36 139
Msa0833700 AT2G45220.1 30.599 317 188 10 54 356 203 501 2.17e-36 138
Msa0833700 AT5G53370.2 30.063 316 193 7 60 359 310 613 3.27e-36 139
Msa0833700 AT2G47030.1 32.867 286 163 6 65 334 282 554 3.79e-36 138
Msa0833700 AT5G53370.1 30.063 316 193 7 60 359 274 577 3.80e-36 138
Msa0833700 AT2G26450.1 27.476 313 201 6 61 359 303 603 1.30e-35 137
Msa0833700 AT3G49220.3 31.329 316 189 8 60 359 330 633 1.53e-35 137
Msa0833700 AT3G49220.1 31.329 316 189 8 60 359 285 588 1.66e-35 137
Msa0833700 AT4G02320.1 27.928 333 211 7 44 359 190 510 2.81e-35 135
Msa0833700 AT2G43050.1 30.072 276 175 5 90 359 245 508 3.43e-35 135
Msa0833700 AT3G14300.1 29.290 338 204 10 41 356 634 958 4.04e-35 137
Msa0833700 AT3G47400.1 30.133 375 215 12 14 359 230 586 8.44e-35 135
Msa0833700 AT3G10720.1 32.340 235 139 5 140 360 24 252 1.24e-34 128
Msa0833700 AT2G47040.1 32.155 283 169 8 65 334 289 561 2.13e-34 134
Msa0833700 AT3G60730.1 31.104 299 179 8 76 359 225 511 7.75e-34 131
Msa0833700 AT5G20860.1 33.333 249 137 8 113 344 251 487 2.91e-32 127
Msa0833700 AT3G62170.1 31.119 286 168 7 65 334 282 554 1.55e-31 125
Msa0833700 AT5G51500.1 27.979 386 223 13 3 356 167 529 1.01e-30 122
Msa0833700 AT1G44980.1 24.405 168 114 3 68 234 86 241 4.63e-11 62.8

Find 126 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTTATTAAAATATTTA+TGG 0.116018 5_4:+6796791 None:intergenic
ACATTCATTGTATTGTTATC+TGG 0.184382 5_4:+6796426 None:intergenic
ACATTCTTTGCAATTGCTTT+TGG 0.241254 5_4:-6796646 Msa0833700:CDS
ACGAAGCTCTTCCTGATAAA+TGG 0.247766 5_4:+6793761 None:intergenic
TTTATAGTAAGAGGTATATT+TGG 0.263269 5_4:+6796882 None:intergenic
CACACGGTGGTTGGGATGAT+TGG 0.283922 5_4:-6794008 Msa0833700:CDS
TTAATTAGGACTGTGTTCTT+TGG 0.291942 5_4:-6793866 Msa0833700:intron
TGTATTTATACTATATATCT+TGG 0.300937 5_4:+6796765 None:intergenic
GTTATCTGGGACGGAATTTA+CGG 0.302481 5_4:+6796440 None:intergenic
GCGGTGGCGTTTCGCATATC+AGG 0.303188 5_4:-6795534 Msa0833700:CDS
GTGAAGCCTGTCTTCTCGTC+TGG 0.316654 5_4:+6794138 None:intergenic
AAACAGCACATCCATTTATC+AGG 0.324261 5_4:-6793772 Msa0833700:CDS
GTCAAGAATAATGCTATATA+TGG 0.331491 5_4:+6793701 None:intergenic
ACAAATAAGAATAGAATGTT+TGG 0.342302 5_4:+6796715 None:intergenic
CAATTCTCAACTCTCTATAA+TGG 0.365946 5_4:-6796685 Msa0833700:exon
ATCCAGCACTGATTTGAATA+AGG 0.375627 5_4:+6796403 None:intergenic
AGGTTCGATCCATTGGTTCT+TGG 0.385472 5_4:+6796527 None:intergenic
CATTCATTGTATTGTTATCT+GGG 0.392536 5_4:+6796427 None:intergenic
TATACTAATATTTACCTTGA+AGG 0.396670 5_4:+6795980 None:intergenic
TAATAAAGAAATGCTTATAA+AGG 0.398499 5_4:-6793482 Msa0833700:three_prime_UTR
CATTTCTTTATTACAATTGA+AGG 0.404631 5_4:+6793492 None:intergenic
TGCAAAGAATATCACCTTCA+AGG 0.406243 5_4:-6795994 Msa0833700:intron
CGTCTTGGACGGACTGGAAC+TGG 0.408345 5_4:+6796463 None:intergenic
CTTTGGAGTGTACAAATGTT+GGG 0.416963 5_4:-6793849 Msa0833700:CDS
TGTAGTAGACAATGTATTGA+AGG 0.418098 5_4:+6796557 None:intergenic
TGCATCCCGGGCATTGGTGC+CGG 0.422340 5_4:+6795567 None:intergenic
CTATATGTAGGACGTGCAAT+GGG 0.422683 5_4:-6794081 Msa0833700:CDS
CGATGCAAGGTGACAGGCTC+AGG 0.427837 5_4:-6794108 Msa0833700:CDS
TTGCCTATCCTGTGCTGCTA+TGG 0.436377 5_4:+6794164 None:intergenic
GTATTTATACTATATATCTT+GGG 0.437627 5_4:+6796766 None:intergenic
ACAGCGCCGGCACCAATGCC+CGG 0.437990 5_4:-6795573 Msa0833700:CDS
TACCGACAGGTTCGATCCAT+TGG 0.440708 5_4:+6796520 None:intergenic
ATTCTTTGCAGAGAAATAGT+TGG 0.443410 5_4:+6796007 None:intergenic
AATCCTTGCATCCCGGGCAT+TGG 0.445967 5_4:+6795561 None:intergenic
CGCTTGCAATCCTTGCATCC+CGG 0.449510 5_4:+6795554 None:intergenic
GAGAAATAACAGTGATTGAA+TGG 0.452312 5_4:-6796106 Msa0833700:CDS
TCAGGAAGAGCTTCGTCAAT+GGG 0.453223 5_4:-6793754 Msa0833700:CDS
TCCCCTTGAAATGTTATGTA+CGG 0.454195 5_4:+6796134 None:intergenic
TGCTGGTAGGCATTACTTCA+AGG 0.455937 5_4:-6795451 Msa0833700:CDS
GTCCTTATTCAAATCAGTGC+TGG 0.456134 5_4:-6796405 Msa0833700:CDS
TTCTCTGGCTGTGGCTTCCA+CGG 0.457319 5_4:-6795498 Msa0833700:CDS
GGGGACAAAGCATACTTCTC+TGG 0.459293 5_4:-6795513 Msa0833700:CDS
TACTATATATCTTGGGAGCA+TGG 0.461957 5_4:+6796773 None:intergenic
ACACGGTGGTTGGGATGATT+GGG 0.462473 5_4:-6794007 Msa0833700:CDS
AGGCAGTACGTGGTGTGTCA+TGG 0.467502 5_4:-6793814 Msa0833700:CDS
ACTTGCATGTTGTTAAATGC+AGG 0.468024 5_4:-6796618 Msa0833700:CDS
AAGCCGTACATAACATTTCA+AGG 0.471438 5_4:-6796137 Msa0833700:CDS
GTGTACAAATGTTGGGGACC+AGG 0.473026 5_4:-6793842 Msa0833700:CDS
GCTTGCAATCCTTGCATCCC+GGG 0.474029 5_4:+6795555 None:intergenic
CCACGTACTGCCTCTGCTCC+TGG 0.474972 5_4:+6793824 None:intergenic
CATTCAATAGCAACAAGGTT+CGG 0.477326 5_4:-6794189 Msa0833700:CDS
CATGATAGAGCTTGTGATCC+TGG 0.477605 5_4:-6796083 Msa0833700:CDS
TACAATGTGTGTAAGTAAAG+AGG 0.481245 5_4:+6796831 None:intergenic
GTACGTAACTGTTGTCCGTT+GGG 0.482645 5_4:+6796059 None:intergenic
CATTGTATTGTTATCTGGGA+CGG 0.482901 5_4:+6796431 None:intergenic
TTCAAGGAATGCTACATTGA+GGG 0.487345 5_4:-6795435 Msa0833700:CDS
GTAATCACAGTTGATATTAA+CGG 0.490404 5_4:-6796492 Msa0833700:CDS
ATTGCACGTCCTACATATAG+TGG 0.491228 5_4:+6794084 None:intergenic
TGAAGGTAGTGTGGTGATGA+TGG 0.492113 5_4:+6796574 None:intergenic
TTACGGCGTCTTGGACGGAC+TGG 0.502096 5_4:+6796457 None:intergenic
TCTTTGGAGTGTACAAATGT+TGG 0.504995 5_4:-6793850 Msa0833700:CDS
GGACGGAATTTACGGCGTCT+TGG 0.507752 5_4:+6796448 None:intergenic
ATCAGGAAGAGCTTCGTCAA+TGG 0.508046 5_4:-6793755 Msa0833700:CDS
GGAATTTACGGCGTCTTGGA+CGG 0.512824 5_4:+6796452 None:intergenic
TACATAACATTTCAAGGGGA+AGG 0.513154 5_4:-6796131 Msa0833700:CDS
AACTGTTGTCCGTTGGGACC+AGG 0.513658 5_4:+6796065 None:intergenic
TATAGTTGCACACGGTGGTT+GGG 0.519022 5_4:-6794016 Msa0833700:CDS
GTTATGTACGGCTTTGTCAC+GGG 0.520170 5_4:+6796146 None:intergenic
ATATAGTTGCACACGGTGGT+TGG 0.521044 5_4:-6794017 Msa0833700:CDS
GCACCAATGCCCGGGATGCA+AGG 0.523172 5_4:-6795564 Msa0833700:CDS
TTCGTACGATGCAAGGTGAC+AGG 0.524057 5_4:-6794114 Msa0833700:CDS
CAGCGCCGGCACCAATGCCC+GGG 0.525664 5_4:-6795572 Msa0833700:CDS
GGCAGTACGTGGTGTGTCAT+GGG 0.529160 5_4:-6793813 Msa0833700:CDS
TGAAGCCTGTCTTCTCGTCT+GGG 0.532273 5_4:+6794139 None:intergenic
ATTACAATTGAAGGAGGTCA+AGG 0.532533 5_4:+6793501 None:intergenic
TGTGTCATGGGCACGAGAGT+TGG 0.536786 5_4:-6793801 Msa0833700:CDS
ACTATATGTAGGACGTGCAA+TGG 0.542419 5_4:-6794082 Msa0833700:CDS
GTTCGGATCCATAGCAGCAC+AGG 0.542754 5_4:-6794172 Msa0833700:CDS
GTGATTACACGGTTACCGAC+AGG 0.543167 5_4:+6796507 None:intergenic
CGGTGGCGTTTCGCATATCA+GGG 0.544327 5_4:-6795533 Msa0833700:CDS
AAAGCATACTTCTCTGGCTG+TGG 0.552260 5_4:-6795507 Msa0833700:CDS
GATCCATAGCAGCACAGGAT+AGG 0.554332 5_4:-6794167 Msa0833700:CDS
TGTTATGTACGGCTTTGTCA+CGG 0.554628 5_4:+6796145 None:intergenic
CAATACCCAGACGAGAAGAC+AGG 0.556786 5_4:-6794144 Msa0833700:CDS
GCTTCGTCAATGGGAGACAT+TGG 0.566621 5_4:-6793745 Msa0833700:CDS
AACTTGATGGTGTAGAGAGA+AGG 0.569823 5_4:-6796177 Msa0833700:intron
TTTGGAGTGTACAAATGTTG+GGG 0.570214 5_4:-6793848 Msa0833700:CDS
AGTACGTAACTGTTGTCCGT+TGG 0.570235 5_4:+6796058 None:intergenic
CTTCAAGGAATGCTACATTG+AGG 0.571058 5_4:-6795436 Msa0833700:CDS
GACACACTTTGTGACGATGC+TGG 0.571568 5_4:-6795468 Msa0833700:CDS
CAAACTCATCCAAGAACCAA+TGG 0.573843 5_4:-6796536 Msa0833700:CDS
ATCATACCTTGTACATGGAT+CGG 0.576866 5_4:+6795385 None:intergenic
ACAGTTGATATTAACGGTGG+AGG 0.587474 5_4:-6796486 Msa0833700:CDS
TTCTTTATTACAATTGAAGG+AGG 0.588089 5_4:+6793495 None:intergenic
CTTCACATTCGTACGATGCA+AGG 0.588588 5_4:-6794121 Msa0833700:CDS
AATGAATCATACCTTGTACA+TGG 0.588920 5_4:+6795380 None:intergenic
GAGACATTGGATCGCACCTA+CGG 0.590531 5_4:-6793732 Msa0833700:CDS
AGCCGTACATAACATTTCAA+GGG 0.591308 5_4:-6796136 Msa0833700:CDS
ACATAACATTTCAAGGGGAA+GGG 0.591823 5_4:-6796130 Msa0833700:CDS
TGCAAAGAATGTAAAAGAGT+AGG 0.602823 5_4:+6796657 None:intergenic
TAATATCAACTGTGATTACA+CGG 0.603000 5_4:+6796496 None:intergenic
ATGTTGGGGACCAGGAGCAG+AGG 0.612194 5_4:-6793834 Msa0833700:CDS
GCTTGTGATCCTGGTCCCAA+CGG 0.619512 5_4:-6796074 Msa0833700:CDS
AAATGGCCGATCCATGTACA+AGG 0.619865 5_4:-6795391 Msa0833700:intron
TTGATGGTGTAGAGAGAAGG+TGG 0.630741 5_4:-6796174 Msa0833700:intron
CACTTTGTGACGATGCTGGT+AGG 0.638179 5_4:-6795464 Msa0833700:CDS
GCCGTACATAACATTTCAAG+GGG 0.638381 5_4:-6796135 Msa0833700:CDS
ATATATGGCTAAGCATCCGT+AGG 0.641001 5_4:+6793716 None:intergenic
GAGCATGTAGAATACAGCGC+CGG 0.642880 5_4:-6795586 Msa0833700:intron
AAAGAGGAAGAACAAATGAG+AGG 0.645359 5_4:+6796847 None:intergenic
CCAGGAGCAGAGGCAGTACG+TGG 0.646245 5_4:-6793824 Msa0833700:CDS
ACAATGTATTGAAGGTAGTG+TGG 0.646502 5_4:+6796565 None:intergenic
CGGGATGCAAGGATTGCAAG+CGG 0.655175 5_4:-6795553 Msa0833700:CDS
AACCAATGGATCGAACCTGT+CGG 0.668325 5_4:-6796522 Msa0833700:CDS
GATGCAAGGATTGCAAGCGG+TGG 0.671605 5_4:-6795550 Msa0833700:CDS
GGCTCAGGTCCACTATATGT+AGG 0.671680 5_4:-6794093 Msa0833700:CDS
ATCACAGTTGATATTAACGG+TGG 0.676561 5_4:-6796489 Msa0833700:CDS
AGCTGCATTCAATAGCAACA+AGG 0.679636 5_4:-6794194 Msa0833700:CDS
TTTGATGATATAGTTGCACA+CGG 0.696006 5_4:-6794024 Msa0833700:CDS
ACAACAGTCTCTCCGCAGAG+AGG 0.708082 5_4:+6793558 None:intergenic
TAATATAGTTATCCTCTCTG+CGG 0.714189 5_4:-6793570 Msa0833700:three_prime_UTR
GGTGGCGTTTCGCATATCAG+GGG 0.723699 5_4:-6795532 Msa0833700:CDS
TATATGTAGGACGTGCAATG+GGG 0.736057 5_4:-6794080 Msa0833700:CDS
ATGGTGTAGAGAGAAGGTGG+TGG 0.744314 5_4:-6796171 Msa0833700:intron
AAAGTGTGTCTTGTGCACCG+TGG 0.789181 5_4:+6795481 None:intergenic
GATGATATAGTTGCACACGG+TGG 0.832583 5_4:-6794021 Msa0833700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTAAAAAAATAATAAAA+AGG + chr5_4:6795393-6795412 None:intergenic 0.0%
!! TATATTTACAATAACAATAT+TGG + chr5_4:6794491-6794510 None:intergenic 10.0%
!!! TTTTAATAATAGTACTTAAT+AGG - chr5_4:6795404-6795423 Msa0833700:CDS 10.0%
!! AATAATTAAAAGAAAAAGAA+TGG + chr5_4:6795901-6795920 None:intergenic 10.0%
!! CATGGTTATTAAAATATTTA+TGG + chr5_4:6793547-6793566 None:intergenic 15.0%
!! GTATTTATACTATATATCTT+GGG + chr5_4:6793572-6793591 None:intergenic 15.0%
!! TGTATTTATACTATATATCT+TGG + chr5_4:6793573-6793592 None:intergenic 15.0%
!! GTATTAATTCTTAATATTGT+TGG - chr5_4:6794081-6794100 Msa0833700:CDS 15.0%
!! TTTGAATGAAAGTAATAATT+TGG - chr5_4:6794124-6794143 Msa0833700:CDS 15.0%
!! TAGTTTATTTATCTCAATTA+TGG - chr5_4:6795004-6795023 Msa0833700:intron 15.0%
!!! ATGATTATTTTATTGAAAAG+TGG - chr5_4:6795524-6795543 Msa0833700:CDS 15.0%
!! AAATTATCCTTATTAACTAT+TGG + chr5_4:6795868-6795887 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG - chr5_4:6795867-6795886 Msa0833700:intron 15.0%
!! TAATAAAAACATCTCTTAAT+TGG - chr5_4:6796101-6796120 Msa0833700:CDS 15.0%
!! TAATAAAGAAATGCTTATAA+AGG - chr5_4:6796853-6796872 Msa0833700:five_prime_UTR 15.0%
!!! TTTATAGTAAGAGGTATATT+TGG + chr5_4:6793456-6793475 None:intergenic 20.0%
!!! TTTGTGTATTTTATAGTAAG+AGG + chr5_4:6793465-6793484 None:intergenic 20.0%
!! ACAAATAAGAATAGAATGTT+TGG + chr5_4:6793623-6793642 None:intergenic 20.0%
!! CTTAATTAATGCTAAAGAAA+AGG + chr5_4:6794031-6794050 None:intergenic 20.0%
!! TATACTAATATTTACCTTGA+AGG + chr5_4:6794358-6794377 None:intergenic 20.0%
!! TTTACAATAACAATATTGGT+AGG + chr5_4:6794487-6794506 None:intergenic 20.0%
!! TAAGCTAAAGAAGTTAAAAA+AGG + chr5_4:6794652-6794671 None:intergenic 20.0%
!! ATTTCCAAAAATGAAATCAA+TGG + chr5_4:6794928-6794947 None:intergenic 20.0%
!!! ATTGATTTCATTTTTGGAAA+TGG - chr5_4:6794927-6794946 Msa0833700:intron 20.0%
!! TAACATCAAAATTCATTAAG+TGG - chr5_4:6795119-6795138 Msa0833700:intron 20.0%
!! TCCAATGTAGAAAAAAATTA+GGG + chr5_4:6795571-6795590 None:intergenic 20.0%
!! TGTTCAACAAAAAAAAAAAC+AGG + chr5_4:6795670-6795689 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + chr5_4:6795781-6795800 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG - chr5_4:6795858-6795877 Msa0833700:intron 20.0%
!! ATTTGTGGATAAACTTAATT+AGG - chr5_4:6796455-6796474 Msa0833700:CDS 20.0%
!!! ATTTTTGTTCTTTTCAACAT+AGG - chr5_4:6796728-6796747 Msa0833700:five_prime_UTR 20.0%
!! CATTTCTTTATTACAATTGA+AGG + chr5_4:6796846-6796865 None:intergenic 20.0%
! TAATATCAACTGTGATTACA+CGG + chr5_4:6793842-6793861 None:intergenic 25.0%
!! GTAATCACAGTTGATATTAA+CGG - chr5_4:6793843-6793862 Msa0833700:CDS 25.0%
! CATTCATTGTATTGTTATCT+GGG + chr5_4:6793911-6793930 None:intergenic 25.0%
! ACATTCATTGTATTGTTATC+TGG + chr5_4:6793912-6793931 None:intergenic 25.0%
! GAATGAAAGTAATAATTTGG+TGG - chr5_4:6794127-6794146 Msa0833700:CDS 25.0%
! TAACTGATAATTAACCTCAA+TGG - chr5_4:6794416-6794435 Msa0833700:intron 25.0%
! AACTGATAATTAACCTCAAT+GGG - chr5_4:6794417-6794436 Msa0833700:intron 25.0%
!! TTTTACCTAAGTTAAAAAGC+TGG + chr5_4:6794675-6794694 None:intergenic 25.0%
!! TATTTTATTGAAAAGTGGTG+TGG - chr5_4:6795529-6795548 Msa0833700:CDS 25.0%
!! ATTTTATTGAAAAGTGGTGT+GGG - chr5_4:6795530-6795549 Msa0833700:CDS 25.0%
! CCAATGTAGAAAAAAATTAG+GGG + chr5_4:6795570-6795589 None:intergenic 25.0%
! CTCCAATGTAGAAAAAAATT+AGG + chr5_4:6795572-6795591 None:intergenic 25.0%
!!! CTAATTTTTTTCTACATTGG+AGG - chr5_4:6795570-6795589 Msa0833700:CDS 25.0%
!!! GAGATGTTTTTATTAAGAGA+TGG + chr5_4:6796096-6796115 None:intergenic 25.0%
! AAACGATAAGAAGGATAAAA+AGG + chr5_4:6796380-6796399 None:intergenic 25.0%
!! TTTTATCCTTCTTATCGTTT+TGG - chr5_4:6796380-6796399 Msa0833700:intron 25.0%
! TAAAATTCAGAAAATCAGTG+TGG + chr5_4:6796423-6796442 None:intergenic 25.0%
! GTCAAGAATAATGCTATATA+TGG + chr5_4:6796637-6796656 None:intergenic 25.0%
!! TTCCTTTAAAAATGTGAGTA+GGG + chr5_4:6796662-6796681 None:intergenic 25.0%
!! TTTCCTTTAAAAATGTGAGT+AGG + chr5_4:6796663-6796682 None:intergenic 25.0%
!! ATATGACAACTCTTTTGTAT+GGG + chr5_4:6796710-6796729 None:intergenic 25.0%
!! AATATGACAACTCTTTTGTA+TGG + chr5_4:6796711-6796730 None:intergenic 25.0%
! TTCTTTATTACAATTGAAGG+AGG + chr5_4:6796843-6796862 None:intergenic 25.0%
TACAATGTGTGTAAGTAAAG+AGG + chr5_4:6793507-6793526 None:intergenic 30.0%
CAATTCTCAACTCTCTATAA+TGG - chr5_4:6793650-6793669 Msa0833700:three_prime_UTR 30.0%
TGCAAAGAATGTAAAAGAGT+AGG + chr5_4:6793681-6793700 None:intergenic 30.0%
! ACATTCTTTGCAATTGCTTT+TGG - chr5_4:6793689-6793708 Msa0833700:three_prime_UTR 30.0%
TGTAGTAGACAATGTATTGA+AGG + chr5_4:6793781-6793800 None:intergenic 30.0%
! TAACTTGCACCATTATTTAG+TGG - chr5_4:6793994-6794013 Msa0833700:CDS 30.0%
CATGTAACACCACTAAATAA+TGG + chr5_4:6794006-6794025 None:intergenic 30.0%
GAGAAATAACAGTGATTGAA+TGG - chr5_4:6794229-6794248 Msa0833700:intron 30.0%
ATTCTTTGCAGAGAAATAGT+TGG + chr5_4:6794331-6794350 None:intergenic 30.0%
CTTTCATTTCAATGTAAGAG+TGG - chr5_4:6794602-6794621 Msa0833700:intron 30.0%
AATGAATCATACCTTGTACA+TGG + chr5_4:6794958-6794977 None:intergenic 30.0%
TGGCAACATTACCTTAATAA+TGG - chr5_4:6795024-6795043 Msa0833700:intron 30.0%
GGCAACATTACCTTAATAAT+GGG - chr5_4:6795025-6795044 Msa0833700:intron 30.0%
CTTTAACGAACCCATTATTA+AGG + chr5_4:6795038-6795057 None:intergenic 30.0%
AGTGTGACAGAGAAATAAAA+AGG + chr5_4:6795084-6795103 None:intergenic 30.0%
AAAACATTGAGATGCGTAAA+GGG - chr5_4:6795147-6795166 Msa0833700:intron 30.0%
TTGCCAAGTGTTTATGAATA+TGG - chr5_4:6795185-6795204 Msa0833700:intron 30.0%
AGTCCATATTCATAAACACT+TGG + chr5_4:6795191-6795210 None:intergenic 30.0%
ACATATATTAGTTCAATGCC+TGG + chr5_4:6795287-6795306 None:intergenic 30.0%
! TTTTGATAGATGATCTCCAT+TGG + chr5_4:6795442-6795461 None:intergenic 30.0%
AATGGAGATCATCTATCAAA+AGG - chr5_4:6795441-6795460 Msa0833700:CDS 30.0%
! TTTTATTGAAAAGTGGTGTG+GGG - chr5_4:6795531-6795550 Msa0833700:CDS 30.0%
!! AAAATTAGGGGTATCTTAAG+TGG + chr5_4:6795558-6795577 None:intergenic 30.0%
!!! CCCCTAATTTTTTTCTACAT+TGG - chr5_4:6795567-6795586 Msa0833700:CDS 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG + chr5_4:6795949-6795968 None:intergenic 30.0%
!! AAACGACACTCATTTTGAAA+CGG - chr5_4:6795950-6795969 Msa0833700:intron 30.0%
!! ATGGAGTAGTTTGACTTTTA+GGG + chr5_4:6796077-6796096 None:intergenic 30.0%
! TTTGATGATATAGTTGCACA+CGG - chr5_4:6796311-6796330 Msa0833700:intron 30.0%
!! TTAACTGTCTTGTTGATTTG+TGG - chr5_4:6796440-6796459 Msa0833700:CDS 30.0%
TTAATTAGGACTGTGTTCTT+TGG - chr5_4:6796469-6796488 Msa0833700:CDS 30.0%
! TTCCCTACTCACATTTTTAA+AGG - chr5_4:6796657-6796676 Msa0833700:CDS 30.0%
TAATATAGTTATCCTCTCTG+CGG - chr5_4:6796765-6796784 Msa0833700:five_prime_UTR 30.0%
AAAGAGGAAGAACAAATGAG+AGG + chr5_4:6793491-6793510 None:intergenic 35.0%
TACTATATATCTTGGGAGCA+TGG + chr5_4:6793565-6793584 None:intergenic 35.0%
ACTTGCATGTTGTTAAATGC+AGG - chr5_4:6793717-6793736 Msa0833700:exon 35.0%
ACAATGTATTGAAGGTAGTG+TGG + chr5_4:6793773-6793792 None:intergenic 35.0%
! ATCACAGTTGATATTAACGG+TGG - chr5_4:6793846-6793865 Msa0833700:CDS 35.0%
CATTGTATTGTTATCTGGGA+CGG + chr5_4:6793907-6793926 None:intergenic 35.0%
!! ATCCAGCACTGATTTGAATA+AGG + chr5_4:6793935-6793954 None:intergenic 35.0%
AAGCCGTACATAACATTTCA+AGG - chr5_4:6794198-6794217 Msa0833700:CDS 35.0%
AGCCGTACATAACATTTCAA+GGG - chr5_4:6794199-6794218 Msa0833700:CDS 35.0%
TCCCCTTGAAATGTTATGTA+CGG + chr5_4:6794204-6794223 None:intergenic 35.0%
TACATAACATTTCAAGGGGA+AGG - chr5_4:6794204-6794223 Msa0833700:CDS 35.0%
ACATAACATTTCAAGGGGAA+GGG - chr5_4:6794205-6794224 Msa0833700:intron 35.0%
TGCAAAGAATATCACCTTCA+AGG - chr5_4:6794341-6794360 Msa0833700:intron 35.0%
ATTAACCTCAATGGGAACAA+AGG - chr5_4:6794425-6794444 Msa0833700:intron 35.0%
TTAACCTCAATGGGAACAAA+GGG - chr5_4:6794426-6794445 Msa0833700:intron 35.0%
!!! GCTTACCAGCTTTTTAACTT+AGG - chr5_4:6794667-6794686 Msa0833700:intron 35.0%
TTCAAGGAATGCTACATTGA+GGG - chr5_4:6794900-6794919 Msa0833700:intron 35.0%
!!! GGCTCCATTGATTTCATTTT+TGG - chr5_4:6794921-6794940 Msa0833700:intron 35.0%
ATCATACCTTGTACATGGAT+CGG + chr5_4:6794953-6794972 None:intergenic 35.0%
! CTTTACGCATCTCAATGTTT+TGG + chr5_4:6795148-6795167 None:intergenic 35.0%
CAAAACATTGAGATGCGTAA+AGG - chr5_4:6795146-6795165 Msa0833700:intron 35.0%
! GGATGCTCTAATGTTCTTTT+GGG + chr5_4:6795318-6795337 None:intergenic 35.0%
! TGGATGCTCTAATGTTCTTT+TGG + chr5_4:6795319-6795338 None:intergenic 35.0%
! CTCACATTTGAGGTCTTAAA+CGG - chr5_4:6795348-6795367 Msa0833700:intron 35.0%
! TCACATTTGAGGTCTTAAAC+GGG - chr5_4:6795349-6795368 Msa0833700:intron 35.0%
AAAAGGTGATGTGTCTATGT+GGG + chr5_4:6795376-6795395 None:intergenic 35.0%
AAAAAGGTGATGTGTCTATG+TGG + chr5_4:6795377-6795396 None:intergenic 35.0%
! ACTCTTTACCATTTAAGACC+CGG + chr5_4:6795609-6795628 None:intergenic 35.0%
!!! GACACTCATTTTGAAACGAA+GGG + chr5_4:6795711-6795730 None:intergenic 35.0%
! TGTGCAGAGTATTAATTGGA+GGG + chr5_4:6795815-6795834 None:intergenic 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG + chr5_4:6795819-6795838 None:intergenic 35.0%
!! GATGGAGTAGTTTGACTTTT+AGG + chr5_4:6796078-6796097 None:intergenic 35.0%
TCTCTTAATTGGAGATTCTC+AGG - chr5_4:6796112-6796131 Msa0833700:CDS 35.0%
CATTCAATAGCAACAAGGTT+CGG - chr5_4:6796146-6796165 Msa0833700:CDS 35.0%
!!! ACTTGTTTTTGTTGTTAGCG+TGG + chr5_4:6796355-6796374 None:intergenic 35.0%
TCATCACCAAAACGATAAGA+AGG + chr5_4:6796389-6796408 None:intergenic 35.0%
!! TCTTTGGAGTGTACAAATGT+TGG - chr5_4:6796485-6796504 Msa0833700:CDS 35.0%
!! CTTTGGAGTGTACAAATGTT+GGG - chr5_4:6796486-6796505 Msa0833700:CDS 35.0%
! TTTGGAGTGTACAAATGTTG+GGG - chr5_4:6796487-6796506 Msa0833700:CDS 35.0%
AAACAGCACATCCATTTATC+AGG - chr5_4:6796563-6796582 Msa0833700:CDS 35.0%
ATTACAATTGAAGGAGGTCA+AGG + chr5_4:6796837-6796856 None:intergenic 35.0%
CAAACTCATCCAAGAACCAA+TGG - chr5_4:6793799-6793818 Msa0833700:CDS 40.0%
! ACAGTTGATATTAACGGTGG+AGG - chr5_4:6793849-6793868 Msa0833700:CDS 40.0%
! GTTATCTGGGACGGAATTTA+CGG + chr5_4:6793898-6793917 None:intergenic 40.0%
GTCCTTATTCAAATCAGTGC+TGG - chr5_4:6793930-6793949 Msa0833700:intron 40.0%
! AGTGCTGGATTCTACAAGTA+TGG - chr5_4:6793945-6793964 Msa0833700:intron 40.0%
GGTGGTGCATATAAACTTGA+TGG - chr5_4:6794145-6794164 Msa0833700:CDS 40.0%
!! AACTTGATGGTGTAGAGAGA+AGG - chr5_4:6794158-6794177 Msa0833700:CDS 40.0%
! TGTTATGTACGGCTTTGTCA+CGG + chr5_4:6794193-6794212 None:intergenic 40.0%
GCCGTACATAACATTTCAAG+GGG - chr5_4:6794200-6794219 Msa0833700:CDS 40.0%
CTTCAAGGAATGCTACATTG+AGG - chr5_4:6794899-6794918 Msa0833700:intron 40.0%
TGCGTAAAGGGATACTAGTA+AGG - chr5_4:6795159-6795178 Msa0833700:intron 40.0%
! CTACCACGTTTTTAATGACC+AGG - chr5_4:6795266-6795285 Msa0833700:intron 40.0%
ATGCCTGGTCATTAAAAACG+TGG + chr5_4:6795272-6795291 None:intergenic 40.0%
TCAAATGTGAGTCTCGATTG+TGG + chr5_4:6795339-6795358 None:intergenic 40.0%
ACAATCGAGACTCACATTTG+AGG - chr5_4:6795338-6795357 Msa0833700:intron 40.0%
ATAGATGATCTCCATTGGAG+AGG + chr5_4:6795437-6795456 None:intergenic 40.0%
!! CTACATTGGAGGTACCTATT+AGG - chr5_4:6795581-6795600 Msa0833700:intron 40.0%
! TTTAAGACCCGGTACCTAAT+AGG + chr5_4:6795598-6795617 None:intergenic 40.0%
!! ATTAGGTACCGGGTCTTAAA+TGG - chr5_4:6795598-6795617 Msa0833700:intron 40.0%
GGGTCTTAAATGGTAAAGAG+TGG - chr5_4:6795608-6795627 Msa0833700:intron 40.0%
!!! CGACACTCATTTTGAAACGA+AGG + chr5_4:6795712-6795731 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG + chr5_4:6795816-6795835 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr5_4:6795954-6795973 Msa0833700:intron 40.0%
AGCTGCATTCAATAGCAACA+AGG - chr5_4:6796141-6796160 Msa0833700:CDS 40.0%
ATTGCACGTCCTACATATAG+TGG + chr5_4:6796254-6796273 None:intergenic 40.0%
ACTATATGTAGGACGTGCAA+TGG - chr5_4:6796253-6796272 Msa0833700:intron 40.0%
CTATATGTAGGACGTGCAAT+GGG - chr5_4:6796254-6796273 Msa0833700:intron 40.0%
TATATGTAGGACGTGCAATG+GGG - chr5_4:6796255-6796274 Msa0833700:intron 40.0%
ACGAAGCTCTTCCTGATAAA+TGG + chr5_4:6796577-6796596 None:intergenic 40.0%
ATATATGGCTAAGCATCCGT+AGG + chr5_4:6796622-6796641 None:intergenic 40.0%
! TGAAGGTAGTGTGGTGATGA+TGG + chr5_4:6793764-6793783 None:intergenic 45.0%
!! AGGTTCGATCCATTGGTTCT+TGG + chr5_4:6793811-6793830 None:intergenic 45.0%
AACCAATGGATCGAACCTGT+CGG - chr5_4:6793813-6793832 Msa0833700:CDS 45.0%
!! TTGATGGTGTAGAGAGAAGG+TGG - chr5_4:6794161-6794180 Msa0833700:CDS 45.0%
! GTTATGTACGGCTTTGTCAC+GGG + chr5_4:6794192-6794211 None:intergenic 45.0%
CATGATAGAGCTTGTGATCC+TGG - chr5_4:6794252-6794271 Msa0833700:intron 45.0%
GTACGTAACTGTTGTCCGTT+GGG + chr5_4:6794279-6794298 None:intergenic 45.0%
AGTACGTAACTGTTGTCCGT+TGG + chr5_4:6794280-6794299 None:intergenic 45.0%
AAAGCATACTTCTCTGGCTG+TGG - chr5_4:6794828-6794847 Msa0833700:intron 45.0%
TGCTGGTAGGCATTACTTCA+AGG - chr5_4:6794884-6794903 Msa0833700:intron 45.0%
AAATGGCCGATCCATGTACA+AGG - chr5_4:6794944-6794963 Msa0833700:intron 45.0%
TAGGTACTCAACCTCTCCAA+TGG - chr5_4:6795423-6795442 Msa0833700:CDS 45.0%
!! TGGAGGTACCTATTAGGTAC+CGG - chr5_4:6795587-6795606 Msa0833700:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr5_4:6795953-6795972 Msa0833700:intron 45.0%
CTTCACATTCGTACGATGCA+AGG - chr5_4:6796214-6796233 Msa0833700:intron 45.0%
GATGATATAGTTGCACACGG+TGG - chr5_4:6796314-6796333 Msa0833700:intron 45.0%
ATATAGTTGCACACGGTGGT+TGG - chr5_4:6796318-6796337 Msa0833700:intron 45.0%
TATAGTTGCACACGGTGGTT+GGG - chr5_4:6796319-6796338 Msa0833700:intron 45.0%
ATCAGGAAGAGCTTCGTCAA+TGG - chr5_4:6796580-6796599 Msa0833700:CDS 45.0%
TCAGGAAGAGCTTCGTCAAT+GGG - chr5_4:6796581-6796600 Msa0833700:CDS 45.0%
TACCGACAGGTTCGATCCAT+TGG + chr5_4:6793818-6793837 None:intergenic 50.0%
GTGATTACACGGTTACCGAC+AGG + chr5_4:6793831-6793850 None:intergenic 50.0%
GGAATTTACGGCGTCTTGGA+CGG + chr5_4:6793886-6793905 None:intergenic 50.0%
ATGGTGTAGAGAGAAGGTGG+TGG - chr5_4:6794164-6794183 Msa0833700:CDS 50.0%
ACCTCAATGGGAACAAAGGG+AGG - chr5_4:6794429-6794448 Msa0833700:intron 50.0%
TCCTCCCTTTGTTCCCATTG+AGG + chr5_4:6794433-6794452 None:intergenic 50.0%
CAATGGGAACAAAGGGAGGA+AGG - chr5_4:6794433-6794452 Msa0833700:intron 50.0%
GAGCATGTAGAATACAGCGC+CGG - chr5_4:6794749-6794768 Msa0833700:intron 50.0%
GGGGACAAAGCATACTTCTC+TGG - chr5_4:6794822-6794841 Msa0833700:intron 50.0%
AAAGTGTGTCTTGTGCACCG+TGG + chr5_4:6794857-6794876 None:intergenic 50.0%
GACACACTTTGTGACGATGC+TGG - chr5_4:6794867-6794886 Msa0833700:intron 50.0%
CACTTTGTGACGATGCTGGT+AGG - chr5_4:6794871-6794890 Msa0833700:intron 50.0%
!! GGAGGTACCTATTAGGTACC+GGG - chr5_4:6795588-6795607 Msa0833700:intron 50.0%
GATCCATAGCAGCACAGGAT+AGG - chr5_4:6796168-6796187 Msa0833700:intron 50.0%
! TTGCCTATCCTGTGCTGCTA+TGG + chr5_4:6796174-6796193 None:intergenic 50.0%
CAATACCCAGACGAGAAGAC+AGG - chr5_4:6796191-6796210 Msa0833700:intron 50.0%
TGAAGCCTGTCTTCTCGTCT+GGG + chr5_4:6796199-6796218 None:intergenic 50.0%
TTCGTACGATGCAAGGTGAC+AGG - chr5_4:6796221-6796240 Msa0833700:intron 50.0%
GGCTCAGGTCCACTATATGT+AGG - chr5_4:6796242-6796261 Msa0833700:intron 50.0%
ACACGGTGGTTGGGATGATT+GGG - chr5_4:6796328-6796347 Msa0833700:intron 50.0%
GTGTACAAATGTTGGGGACC+AGG - chr5_4:6796493-6796512 Msa0833700:CDS 50.0%
GCTTCGTCAATGGGAGACAT+TGG - chr5_4:6796590-6796609 Msa0833700:CDS 50.0%
GAGACATTGGATCGCACCTA+CGG - chr5_4:6796603-6796622 Msa0833700:CDS 50.0%
GGACGGAATTTACGGCGTCT+TGG + chr5_4:6793890-6793909 None:intergenic 55.0%
GCTTGTGATCCTGGTCCCAA+CGG - chr5_4:6794261-6794280 Msa0833700:intron 55.0%
AACTGTTGTCCGTTGGGACC+AGG + chr5_4:6794273-6794292 None:intergenic 55.0%
AATCCTTGCATCCCGGGCAT+TGG + chr5_4:6794777-6794796 None:intergenic 55.0%
GCTTGCAATCCTTGCATCCC+GGG + chr5_4:6794783-6794802 None:intergenic 55.0%
CGCTTGCAATCCTTGCATCC+CGG + chr5_4:6794784-6794803 None:intergenic 55.0%
CGGGATGCAAGGATTGCAAG+CGG - chr5_4:6794782-6794801 Msa0833700:intron 55.0%
GATGCAAGGATTGCAAGCGG+TGG - chr5_4:6794785-6794804 Msa0833700:intron 55.0%
CGGTGGCGTTTCGCATATCA+GGG - chr5_4:6794802-6794821 Msa0833700:intron 55.0%
GGTGGCGTTTCGCATATCAG+GGG - chr5_4:6794803-6794822 Msa0833700:intron 55.0%
TTCTCTGGCTGTGGCTTCCA+CGG - chr5_4:6794837-6794856 Msa0833700:intron 55.0%
GTTCGGATCCATAGCAGCAC+AGG - chr5_4:6796163-6796182 Msa0833700:CDS 55.0%
GTGAAGCCTGTCTTCTCGTC+TGG + chr5_4:6796200-6796219 None:intergenic 55.0%
CACACGGTGGTTGGGATGAT+TGG - chr5_4:6796327-6796346 Msa0833700:intron 55.0%
AGGCAGTACGTGGTGTGTCA+TGG - chr5_4:6796521-6796540 Msa0833700:CDS 55.0%
GGCAGTACGTGGTGTGTCAT+GGG - chr5_4:6796522-6796541 Msa0833700:CDS 55.0%
TGTGTCATGGGCACGAGAGT+TGG - chr5_4:6796534-6796553 Msa0833700:CDS 55.0%
ACAACAGTCTCTCCGCAGAG+AGG + chr5_4:6796780-6796799 None:intergenic 55.0%
!! CGTCTTGGACGGACTGGAAC+TGG + chr5_4:6793875-6793894 None:intergenic 60.0%
TTACGGCGTCTTGGACGGAC+TGG + chr5_4:6793881-6793900 None:intergenic 60.0%
GCGGTGGCGTTTCGCATATC+AGG - chr5_4:6794801-6794820 Msa0833700:intron 60.0%
CGATGCAAGGTGACAGGCTC+AGG - chr5_4:6796227-6796246 Msa0833700:intron 60.0%
ATGTTGGGGACCAGGAGCAG+AGG - chr5_4:6796501-6796520 Msa0833700:CDS 60.0%
!! TGCATCCCGGGCATTGGTGC+CGG + chr5_4:6794771-6794790 None:intergenic 65.0%
GCACCAATGCCCGGGATGCA+AGG - chr5_4:6794771-6794790 Msa0833700:intron 65.0%
CCACGTACTGCCTCTGCTCC+TGG + chr5_4:6796514-6796533 None:intergenic 65.0%
CCAGGAGCAGAGGCAGTACG+TGG - chr5_4:6796511-6796530 Msa0833700:CDS 65.0%
!! ACAGCGCCGGCACCAATGCC+CGG - chr5_4:6794762-6794781 Msa0833700:intron 70.0%
!! CAGCGCCGGCACCAATGCCC+GGG - chr5_4:6794763-6794782 Msa0833700:intron 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 6793452 6796905 6793452 ID=Msa0833700;Name=Msa0833700
chr5_4 mRNA 6793452 6796905 6793452 ID=Msa0833700-mRNA-1;Parent=Msa0833700;Name=Msa0833700-mRNA-1;_AED=0.03;_eAED=0.03;_QI=217|1|1|1|1|1|5|272|361
chr5_4 exon 6793452 6793880 6793452 ID=Msa0833700-mRNA-1:exon:3038;Parent=Msa0833700-mRNA-1
chr5_4 exon 6793985 6794223 6793985 ID=Msa0833700-mRNA-1:exon:3037;Parent=Msa0833700-mRNA-1
chr5_4 exon 6795392 6795598 6795392 ID=Msa0833700-mRNA-1:exon:3036;Parent=Msa0833700-mRNA-1
chr5_4 exon 6795995 6796184 6795995 ID=Msa0833700-mRNA-1:exon:3035;Parent=Msa0833700-mRNA-1
chr5_4 exon 6796396 6796905 6796396 ID=Msa0833700-mRNA-1:exon:3034;Parent=Msa0833700-mRNA-1
chr5_4 five_prime_UTR 6796689 6796905 6796689 ID=Msa0833700-mRNA-1:five_prime_utr;Parent=Msa0833700-mRNA-1
chr5_4 CDS 6796396 6796688 6796396 ID=Msa0833700-mRNA-1:cds;Parent=Msa0833700-mRNA-1
chr5_4 CDS 6795995 6796184 6795995 ID=Msa0833700-mRNA-1:cds;Parent=Msa0833700-mRNA-1
chr5_4 CDS 6795392 6795598 6795392 ID=Msa0833700-mRNA-1:cds;Parent=Msa0833700-mRNA-1
chr5_4 CDS 6793985 6794223 6793985 ID=Msa0833700-mRNA-1:cds;Parent=Msa0833700-mRNA-1
chr5_4 CDS 6793724 6793880 6793724 ID=Msa0833700-mRNA-1:cds;Parent=Msa0833700-mRNA-1
chr5_4 three_prime_UTR 6793452 6793723 6793452 ID=Msa0833700-mRNA-1:three_prime_utr;Parent=Msa0833700-mRNA-1
Gene Sequence

>Msa0833700

ATGGATACACCTACTCTTTTACATTCTTTGCAATTGCTTTTGGTTTTCACTTGCATGTTGTTAAATGCAGGAATAGTGACACTAGCTGCACGCCATCATCACCACACTACCTTCAATACATTGTCTACTACAAACTCATCCAAGAACCAATGGATCGAACCTGTCGGTAACCGTGTAATCACAGTTGATATTAACGGTGGAGGCCAGTTCCAGTCCGTCCAAGACGCCGTAAATTCCGTCCCAGATAACAATACAATGAATGTCCTTATTCAAATCAGTGCTGGATTCTACAAAGAGAAGGTGGTGGTTCCCGTGACAAAGCCGTACATAACATTTCAAGGGGAAGGGAGAGAAATAACAGTGATTGAATGGCATGATAGAGCTTGTGATCCTGGTCCCAACGGACAACAGTTACGTACTTATAGAACTGCTTCTGTTACTGTCTTTGCCAACTATTTCTCTGCAAAGAATATCACCTTCAAGAATACAGCGCCGGCACCAATGCCCGGGATGCAAGGATTGCAAGCGGTGGCGTTTCGCATATCAGGGGACAAAGCATACTTCTCTGGCTGTGGCTTCCACGGTGCACAAGACACACTTTGTGACGATGCTGGTAGGCATTACTTCAAGGAATGCTACATTGAGGGCTCCATTGATTTCATTTTTGGAAATGGCCGATCCATGTACAAGGATTGTGAGCTGCATTCAATAGCAACAAGGTTCGGATCCATAGCAGCACAGGATAGGCAATACCCAGACGAGAAGACAGGCTTCACATTCGTACGATGCAAGGTGACAGGCTCAGGTCCACTATATGTAGGACGTGCAATGGGGCAATACTCAAGAATAGTTTATGCATATACATACTTTGATGATATAGTTGCACACGGTGGTTGGGATGATTGGGACCACGCTAACAACAAAAACAAGACTGTGTTCTTTGGAGTGTACAAATGTTGGGGACCAGGAGCAGAGGCAGTACGTGGTGTGTCATGGGCACGAGAGTTGGATTTCGAAACAGCACATCCATTTATCAGGAAGAGCTTCGTCAATGGGAGACATTGGATCGCACCTACGGATGCTTAG
Protein sequence

>Msa0833700

MDTPTLLHSLQLLLVFTCMLLNAGIVTLAARHHHHTTFNTLSTTNSSKNQWIEPVGNRVITVDINGGGQFQSVQDAVNSVPDNNTMNVLIQISAGFYKEKVVVPVTKPYITFQGEGREITVIEWHDRACDPGPNGQQLRTYRTASVTVFANYFSAKNITFKNTAPAPMPGMQGLQAVAFRISGDKAYFSGCGFHGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRQYPDEKTGFTFVRCKVTGSGPLYVGRAMGQYSRIVYAYTYFDDIVAHGGWDDWDHANNKNKTVFFGVYKCWGPGAEAVRGVSWARELDFETAHPFIRKSFVNGRHWIAPTDA*