Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581360 | XP_003610142.1 | 94.700 | 283 | 15 | 0 | 1 | 283 | 1 | 283 | 0.0 | 558 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581360 | sp|Q38910|XTH23_ARATH | 73.498 | 283 | 72 | 2 | 1 | 280 | 1 | 283 | 4.32e-156 | 439 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581360 | G7JVI0 | 94.700 | 283 | 15 | 0 | 1 | 283 | 1 | 283 | 0.0 | 558 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0266220 | Msa0581360 | 0.805815 | 1.112623e-49 | -8.615850e-47 |
| Msa0581360 | Msa1221280 | 0.944006 | 4.293967e-103 | -8.615850e-47 |
| Msa0581360 | Msa1337440 | 0.844860 | 5.817877e-59 | -8.615850e-47 |
| Msa0581360 | Msa1360260 | 0.803447 | 3.471750e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581360 | MtrunA17_Chr4g0072611 | 94.700 | 283 | 15 | 0 | 1 | 283 | 1 | 283 | 0.0 | 558 |
| Msa0581360 | MtrunA17_Chr7g0258911 | 91.166 | 283 | 24 | 1 | 1 | 283 | 1 | 282 | 0.0 | 512 |
| Msa0581360 | MtrunA17_Chr4g0072671 | 84.411 | 263 | 37 | 2 | 22 | 280 | 23 | 285 | 3.69e-163 | 454 |
| Msa0581360 | MtrunA17_Chr4g0072601 | 73.310 | 281 | 71 | 1 | 4 | 280 | 3 | 283 | 3.17e-156 | 437 |
| Msa0581360 | MtrunA17_Chr4g0071341 | 74.815 | 270 | 67 | 1 | 14 | 282 | 14 | 283 | 8.29e-147 | 412 |
| Msa0581360 | MtrunA17_Chr2g0299061 | 69.675 | 277 | 79 | 1 | 9 | 280 | 10 | 286 | 6.38e-143 | 403 |
| Msa0581360 | MtrunA17_Chr8g0377841 | 71.691 | 272 | 72 | 1 | 9 | 280 | 15 | 281 | 8.35e-143 | 402 |
| Msa0581360 | MtrunA17_Chr3g0122031 | 70.107 | 281 | 77 | 2 | 1 | 281 | 5 | 278 | 1.48e-141 | 399 |
| Msa0581360 | MtrunA17_Chr3g0122021 | 70.107 | 281 | 77 | 2 | 1 | 281 | 5 | 278 | 1.60e-141 | 399 |
| Msa0581360 | MtrunA17_Chr4g0072681 | 73.663 | 243 | 56 | 2 | 46 | 280 | 1 | 243 | 3.80e-129 | 367 |
| Msa0581360 | MtrunA17_Chr8g0389641 | 64.207 | 271 | 54 | 2 | 11 | 281 | 16 | 243 | 2.42e-123 | 352 |
| Msa0581360 | MtrunA17_Chr5g0443391 | 55.678 | 273 | 116 | 1 | 9 | 281 | 12 | 279 | 1.26e-108 | 316 |
| Msa0581360 | MtrunA17_Chr6g0486581 | 52.330 | 279 | 126 | 3 | 6 | 280 | 8 | 283 | 3.38e-103 | 302 |
| Msa0581360 | MtrunA17_Chr6g0461731 | 53.191 | 282 | 118 | 4 | 10 | 280 | 32 | 310 | 3.20e-102 | 301 |
| Msa0581360 | MtrunA17_Chr7g0252631 | 54.851 | 268 | 111 | 4 | 17 | 281 | 28 | 288 | 5.47e-102 | 299 |
| Msa0581360 | MtrunA17_Chr7g0252621 | 52.448 | 286 | 124 | 5 | 1 | 281 | 10 | 288 | 9.87e-102 | 299 |
| Msa0581360 | MtrunA17_Chr6g0461761 | 53.546 | 282 | 117 | 4 | 10 | 280 | 9 | 287 | 1.45e-101 | 298 |
| Msa0581360 | MtrunA17_Chr3g0096651 | 54.023 | 261 | 115 | 2 | 22 | 281 | 27 | 283 | 1.21e-100 | 296 |
| Msa0581360 | MtrunA17_Chr7g0252601 | 50.859 | 291 | 130 | 4 | 1 | 281 | 1 | 288 | 2.21e-100 | 295 |
| Msa0581360 | MtrunA17_Chr2g0323161 | 51.095 | 274 | 120 | 5 | 17 | 280 | 17 | 286 | 3.84e-98 | 290 |
| Msa0581360 | MtrunA17_Chr4g0023961 | 49.296 | 284 | 132 | 4 | 6 | 280 | 8 | 288 | 9.70e-97 | 286 |
| Msa0581360 | MtrunA17_Chr4g0054451 | 49.643 | 280 | 132 | 6 | 6 | 280 | 14 | 289 | 3.32e-96 | 285 |
| Msa0581360 | MtrunA17_Chr4g0054431 | 50.179 | 279 | 130 | 6 | 6 | 280 | 14 | 287 | 1.16e-95 | 283 |
| Msa0581360 | MtrunA17_Chr1g0206681 | 49.446 | 271 | 133 | 3 | 15 | 282 | 24 | 293 | 3.58e-92 | 275 |
| Msa0581360 | MtrunA17_Chr1g0206081 | 47.687 | 281 | 135 | 5 | 11 | 283 | 21 | 297 | 2.52e-86 | 260 |
| Msa0581360 | MtrunA17_Chr8g0390201 | 54.545 | 198 | 86 | 1 | 90 | 283 | 15 | 212 | 2.26e-77 | 234 |
| Msa0581360 | MtrunA17_Chr8g0340001 | 46.957 | 230 | 111 | 5 | 56 | 280 | 8 | 231 | 3.66e-68 | 211 |
| Msa0581360 | MtrunA17_Chr4g0072641 | 74.603 | 126 | 18 | 2 | 155 | 280 | 37 | 148 | 2.47e-61 | 191 |
| Msa0581360 | MtrunA17_Chr4g0028221 | 41.132 | 265 | 139 | 7 | 24 | 280 | 49 | 304 | 4.60e-61 | 195 |
| Msa0581360 | MtrunA17_Chr7g0264731 | 40.377 | 265 | 141 | 6 | 24 | 280 | 36 | 291 | 1.33e-59 | 191 |
| Msa0581360 | MtrunA17_Chr2g0327411 | 40.755 | 265 | 141 | 7 | 24 | 280 | 53 | 309 | 5.24e-59 | 190 |
| Msa0581360 | MtrunA17_Chr2g0303861 | 41.985 | 262 | 135 | 9 | 25 | 281 | 29 | 278 | 8.19e-59 | 189 |
| Msa0581360 | MtrunA17_Chr1g0180451 | 38.868 | 265 | 145 | 6 | 24 | 280 | 39 | 294 | 9.02e-58 | 187 |
| Msa0581360 | MtrunA17_Chr8g0375981 | 38.346 | 266 | 148 | 7 | 25 | 280 | 31 | 290 | 1.99e-56 | 184 |
| Msa0581360 | MtrunA17_Chr2g0303721 | 39.223 | 283 | 150 | 9 | 3 | 277 | 6 | 274 | 5.44e-56 | 181 |
| Msa0581360 | MtrunA17_Chr1g0161951 | 40.310 | 258 | 137 | 8 | 33 | 280 | 41 | 291 | 1.97e-54 | 179 |
| Msa0581360 | MtrunA17_Chr7g0235541 | 45.161 | 217 | 105 | 6 | 72 | 282 | 1 | 209 | 1.29e-53 | 173 |
| Msa0581360 | MtrunA17_Chr8g0365231 | 36.364 | 264 | 155 | 6 | 29 | 281 | 50 | 311 | 2.17e-52 | 173 |
| Msa0581360 | MtrunA17_Chr5g0410621 | 36.782 | 261 | 151 | 9 | 33 | 282 | 50 | 307 | 1.45e-49 | 167 |
| Msa0581360 | MtrunA17_Chr8g0382041 | 35.401 | 274 | 146 | 10 | 25 | 280 | 34 | 294 | 3.87e-44 | 152 |
| Msa0581360 | MtrunA17_Chr4g0072631 | 76.190 | 63 | 15 | 0 | 10 | 72 | 16 | 78 | 2.96e-27 | 102 |
| Msa0581360 | MtrunA17_Chr8g0374231 | 44.444 | 108 | 51 | 4 | 181 | 283 | 1 | 104 | 1.92e-23 | 92.0 |
| Msa0581360 | MtrunA17_Chr8g0390181 | 47.826 | 92 | 43 | 2 | 1 | 92 | 7 | 93 | 2.66e-17 | 75.5 |
| Msa0581360 | MtrunA17_Chr8g0350711 | 32.184 | 174 | 110 | 4 | 47 | 218 | 54 | 221 | 1.84e-14 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581360 | AT4G25810.1 | 73.498 | 283 | 72 | 2 | 1 | 280 | 1 | 283 | 4.40e-157 | 439 |
| Msa0581360 | AT5G57550.1 | 69.534 | 279 | 82 | 1 | 3 | 281 | 8 | 283 | 1.27e-149 | 420 |
| Msa0581360 | AT5G57560.1 | 69.474 | 285 | 78 | 3 | 1 | 280 | 1 | 281 | 1.36e-149 | 420 |
| Msa0581360 | AT4G30270.1 | 68.116 | 276 | 70 | 2 | 9 | 280 | 4 | 265 | 5.16e-143 | 403 |
| Msa0581360 | AT3G23730.1 | 66.078 | 283 | 92 | 1 | 3 | 281 | 5 | 287 | 3.70e-141 | 399 |
| Msa0581360 | AT4G14130.1 | 69.118 | 272 | 83 | 1 | 11 | 281 | 14 | 285 | 3.14e-140 | 397 |
| Msa0581360 | AT5G48070.1 | 64.130 | 276 | 92 | 3 | 8 | 280 | 10 | 281 | 2.68e-137 | 389 |
| Msa0581360 | AT5G57530.1 | 68.077 | 260 | 83 | 0 | 23 | 282 | 25 | 284 | 4.38e-135 | 383 |
| Msa0581360 | AT5G57540.1 | 66.284 | 261 | 88 | 0 | 22 | 282 | 23 | 283 | 2.86e-133 | 379 |
| Msa0581360 | AT4G25820.1 | 66.541 | 266 | 89 | 0 | 17 | 282 | 22 | 287 | 6.23e-132 | 375 |
| Msa0581360 | AT2G18800.1 | 62.676 | 284 | 93 | 4 | 10 | 280 | 13 | 296 | 3.76e-131 | 374 |
| Msa0581360 | AT4G30290.1 | 62.724 | 279 | 97 | 3 | 5 | 280 | 2 | 276 | 7.91e-125 | 357 |
| Msa0581360 | AT4G30280.1 | 62.500 | 272 | 97 | 2 | 10 | 280 | 14 | 281 | 4.17e-124 | 355 |
| Msa0581360 | AT1G65310.2 | 64.504 | 262 | 88 | 2 | 20 | 280 | 46 | 303 | 3.34e-123 | 354 |
| Msa0581360 | AT1G65310.1 | 64.504 | 262 | 88 | 2 | 20 | 280 | 24 | 281 | 5.75e-123 | 352 |
| Msa0581360 | AT5G13870.2 | 53.546 | 282 | 121 | 4 | 7 | 281 | 10 | 288 | 2.86e-102 | 300 |
| Msa0581360 | AT5G13870.1 | 53.546 | 282 | 121 | 4 | 7 | 281 | 10 | 288 | 2.86e-102 | 300 |
| Msa0581360 | AT5G13870.3 | 53.546 | 282 | 121 | 4 | 7 | 281 | 10 | 288 | 2.86e-102 | 300 |
| Msa0581360 | AT2G06850.1 | 51.056 | 284 | 133 | 3 | 1 | 281 | 11 | 291 | 9.55e-97 | 286 |
| Msa0581360 | AT4G03210.1 | 49.306 | 288 | 133 | 6 | 1 | 281 | 4 | 285 | 5.28e-96 | 285 |
| Msa0581360 | AT5G65730.1 | 46.853 | 286 | 140 | 6 | 1 | 281 | 13 | 291 | 1.18e-94 | 281 |
| Msa0581360 | AT4G37800.1 | 47.099 | 293 | 138 | 6 | 1 | 280 | 1 | 289 | 3.16e-92 | 275 |
| Msa0581360 | AT4G28850.1 | 48.043 | 281 | 133 | 4 | 11 | 280 | 9 | 287 | 3.61e-92 | 275 |
| Msa0581360 | AT2G14620.1 | 46.429 | 280 | 146 | 3 | 6 | 282 | 18 | 296 | 2.98e-89 | 268 |
| Msa0581360 | AT4G03210.2 | 53.527 | 241 | 99 | 5 | 47 | 281 | 24 | 257 | 6.57e-89 | 265 |
| Msa0581360 | AT2G06850.2 | 55.217 | 230 | 97 | 3 | 55 | 281 | 5 | 231 | 1.21e-88 | 264 |
| Msa0581360 | AT1G11545.1 | 47.099 | 293 | 138 | 7 | 2 | 283 | 16 | 302 | 2.78e-87 | 263 |
| Msa0581360 | AT4G13090.1 | 45.878 | 279 | 144 | 4 | 8 | 281 | 13 | 289 | 7.47e-81 | 246 |
| Msa0581360 | AT2G14620.2 | 50.000 | 230 | 111 | 3 | 56 | 282 | 18 | 246 | 9.64e-79 | 239 |
| Msa0581360 | AT4G13080.1 | 43.253 | 289 | 153 | 4 | 1 | 281 | 7 | 292 | 3.81e-77 | 236 |
| Msa0581360 | AT2G14620.3 | 50.679 | 221 | 105 | 3 | 65 | 282 | 16 | 235 | 8.76e-77 | 234 |
| Msa0581360 | AT3G25050.1 | 44.788 | 259 | 136 | 4 | 25 | 280 | 35 | 289 | 4.14e-76 | 234 |
| Msa0581360 | AT2G36870.1 | 41.509 | 265 | 138 | 6 | 24 | 280 | 42 | 297 | 4.67e-60 | 193 |
| Msa0581360 | AT3G44990.1 | 41.288 | 264 | 138 | 6 | 25 | 280 | 39 | 293 | 5.41e-59 | 190 |
| Msa0581360 | AT1G32170.1 | 38.934 | 244 | 135 | 7 | 47 | 280 | 54 | 293 | 3.01e-54 | 179 |
| Msa0581360 | AT1G14720.1 | 35.616 | 292 | 169 | 9 | 3 | 280 | 4 | 290 | 1.31e-53 | 177 |
| Msa0581360 | AT1G10550.1 | 36.398 | 261 | 147 | 6 | 36 | 280 | 52 | 309 | 2.30e-52 | 174 |
| Msa0581360 | AT2G01850.1 | 33.333 | 291 | 177 | 8 | 3 | 280 | 4 | 290 | 5.52e-49 | 166 |
| Msa0581360 | AT3G48580.1 | 36.940 | 268 | 145 | 10 | 15 | 280 | 28 | 273 | 1.65e-47 | 160 |
| Msa0581360 | AT4G18990.1 | 35.231 | 281 | 145 | 10 | 31 | 280 | 38 | 312 | 8.33e-46 | 158 |
| Msa0581360 | AT3G48580.3 | 36.842 | 228 | 125 | 8 | 55 | 280 | 4 | 214 | 3.85e-41 | 142 |
| Msa0581360 | AT4G18990.2 | 36.634 | 202 | 104 | 7 | 98 | 280 | 42 | 238 | 3.40e-37 | 133 |
| Msa0581360 | AT2G36870.2 | 44.604 | 139 | 67 | 3 | 24 | 154 | 42 | 178 | 1.09e-30 | 113 |
| Msa0581360 | AT3G48580.2 | 32.090 | 268 | 138 | 11 | 15 | 280 | 28 | 253 | 1.18e-30 | 115 |
Find 70 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGATAGACTTTGAATTCT+TGG | 0.224719 | 4_1:+86739450 | Msa0581360:CDS |
| ATGTTCTTGAATTCTCTAAT+TGG | 0.274937 | 4_1:-86739745 | None:intergenic |
| TTCAAATATACTTACATAAT+AGG | 0.274974 | 4_1:-86739309 | None:intergenic |
| CTCTCTCTTGATAAATATTC+TGG | 0.281081 | 4_1:+86739194 | Msa0581360:CDS |
| TGAGGATCTATTCTAGTCTT+TGG | 0.281081 | 4_1:+86739806 | Msa0581360:CDS |
| AATGCATGCACTGTGTCTTC+TGG | 0.301674 | 4_1:+86739919 | Msa0581360:CDS |
| TTTGTTATAGTTGAGAAATA+TGG | 0.302317 | 4_1:-86738974 | None:intergenic |
| TTTGCCTTGGCTAAACATAT+TGG | 0.338463 | 4_1:-86739506 | None:intergenic |
| GTCTTTGGAATGCTGATGAT+TGG | 0.342081 | 4_1:+86739821 | Msa0581360:CDS |
| GAGATAGACTTTGAATTCTT+GGG | 0.388826 | 4_1:+86739451 | Msa0581360:CDS |
| TTGTTATAGTTGAGAAATAT+GGG | 0.388984 | 4_1:-86738973 | None:intergenic |
| CTTGATAAATATTCTGGCTC+TGG | 0.390937 | 4_1:+86739200 | Msa0581360:CDS |
| TGTGTAAGTGATCATTATAA+AGG | 0.397248 | 4_1:+86740212 | Msa0581360:three_prime_UTR |
| GAGAGCAACAATTCTACCTT+TGG | 0.408360 | 4_1:+86739536 | Msa0581360:CDS |
| ATGCAACTTAAACTTGTTCC+TGG | 0.420276 | 4_1:+86739263 | Msa0581360:CDS |
| CAAGAATGCAGGATGACTTA+GGG | 0.422302 | 4_1:+86740096 | Msa0581360:CDS |
| TCAAGAATGCAGGATGACTT+AGG | 0.428486 | 4_1:+86740095 | Msa0581360:CDS |
| TGAAGCAGTGAAAGGAGCTT+TGG | 0.428854 | 4_1:-86739878 | None:intergenic |
| CACAATCTGATAAGAATGAT+TGG | 0.435316 | 4_1:+86739962 | Msa0581360:CDS |
| GCTCTCTGTCACCTTTGCCT+TGG | 0.439694 | 4_1:-86739519 | None:intergenic |
| CTTGTTCCTGGCAACTCTGC+TGG | 0.467097 | 4_1:+86739275 | Msa0581360:CDS |
| TGATAACAAAAGATTTCCTC+AGG | 0.471429 | 4_1:+86740065 | Msa0581360:CDS |
| GGCTAAACATATTGGTATGA+AGG | 0.473300 | 4_1:-86739498 | None:intergenic |
| TCTTTGGAATGCTGATGATT+GGG | 0.473720 | 4_1:+86739822 | Msa0581360:CDS |
| CTAATTCAGCTATCATCAAA+AGG | 0.477234 | 4_1:+86739415 | Msa0581360:intron |
| ACTGTTGCAATTGCCTATGC+TGG | 0.488876 | 4_1:+86739095 | Msa0581360:CDS |
| AGACTAGAATAGATCCTCAT+TGG | 0.496993 | 4_1:-86739802 | None:intergenic |
| AAGTTCAAATACTTTAAGAC+TGG | 0.498828 | 4_1:-86739006 | None:intergenic |
| GAGGACTTGTTAAGACAGAT+TGG | 0.501266 | 4_1:+86739854 | Msa0581360:CDS |
| TAGAATAGATCCTCATTGGC+TGG | 0.507706 | 4_1:-86739798 | None:intergenic |
| CAAGAGAGAGAGTAAGAAGT+TGG | 0.519079 | 4_1:-86739181 | None:intergenic |
| ATCAAGACTTTGATATAACA+TGG | 0.525397 | 4_1:+86739126 | Msa0581360:CDS |
| ATATTGGTATGAAGGATGTA+AGG | 0.534436 | 4_1:-86739490 | None:intergenic |
| ATCTCCTTGTAAGTAGTCAA+TGG | 0.538861 | 4_1:+86739031 | Msa0581360:exon |
| ACAGTGCCAGCAGAGTTGCC+AGG | 0.543526 | 4_1:-86739281 | None:intergenic |
| TTCACACATACTCAATTCTG+TGG | 0.546538 | 4_1:+86739578 | Msa0581360:CDS |
| TACAATTATACAACATGTTG+AGG | 0.546546 | 4_1:-86740138 | None:intergenic |
| AGGGTCTTCCTCAAGAATGC+AGG | 0.547862 | 4_1:+86740085 | Msa0581360:CDS |
| CAAGTCTTGAGAGGCTCAAG+TGG | 0.563861 | 4_1:+86740007 | Msa0581360:CDS |
| TCAAGACTTTGATATAACAT+GGG | 0.567537 | 4_1:+86739127 | Msa0581360:CDS |
| ATCATCAAAAGGAGGTGCAT+GGG | 0.570048 | 4_1:+86739426 | Msa0581360:CDS |
| AGTGCCATTGACTACTTACA+AGG | 0.576524 | 4_1:-86739035 | None:intergenic |
| TATCATCAAAAGGAGGTGCA+TGG | 0.582020 | 4_1:+86739425 | Msa0581360:CDS |
| TCTTCTGGAATATCATCTTG+TGG | 0.584435 | 4_1:+86739934 | Msa0581360:CDS |
| CATACCAATATGTTTAGCCA+AGG | 0.585494 | 4_1:+86739502 | Msa0581360:CDS |
| AATTAGAGAATTCAAGAACA+TGG | 0.587989 | 4_1:+86739747 | Msa0581360:CDS |
| CAAGACTTTGATATAACATG+GGG | 0.592977 | 4_1:+86739128 | Msa0581360:CDS |
| TTTCTGTATGAAGCAGTGAA+AGG | 0.596238 | 4_1:-86739886 | None:intergenic |
| CTGCAGTTGGGTCAAACCAA+AGG | 0.596298 | 4_1:-86739552 | None:intergenic |
| ATGTGTGAAAATCTGCAGTT+GGG | 0.606525 | 4_1:-86739564 | None:intergenic |
| ACTTGTAGAATCTAGCTCCT+CGG | 0.610078 | 4_1:-86739989 | None:intergenic |
| TATGTGTGAAAATCTGCAGT+TGG | 0.616665 | 4_1:-86739565 | None:intergenic |
| GATGATTGGGCTACAAGAGG+AGG | 0.618195 | 4_1:+86739835 | Msa0581360:CDS |
| AGTACTTACACAATGCGTTG+TGG | 0.625079 | 4_1:-86739604 | None:intergenic |
| TTCAAGAACATGGAATCAAG+TGG | 0.625602 | 4_1:+86739757 | Msa0581360:CDS |
| GCTGATGATTGGGCTACAAG+AGG | 0.628982 | 4_1:+86739832 | Msa0581360:CDS |
| GAATTCTTGGGAAATTTGAG+TGG | 0.630242 | 4_1:+86739463 | Msa0581360:CDS |
| TAGATTCTACAAGTCTTGAG+AGG | 0.640770 | 4_1:+86739998 | Msa0581360:CDS |
| AAGAATGCAGGATGACTTAG+GGG | 0.644048 | 4_1:+86740097 | Msa0581360:CDS |
| TAAGTCATCCTGCATTCTTG+AGG | 0.647584 | 4_1:-86740093 | None:intergenic |
| AATATGTTTAGCCAAGGCAA+AGG | 0.662699 | 4_1:+86739508 | Msa0581360:CDS |
| GGTCGTGCTAAAATACTCAA+CGG | 0.673213 | 4_1:+86739155 | Msa0581360:CDS |
| AAGTCTTGAGAGGCTCAAGT+GGG | 0.675842 | 4_1:+86740008 | Msa0581360:CDS |
| AGACTTTGATATAACATGGG+GGG | 0.676311 | 4_1:+86739130 | Msa0581360:CDS |
| GATAACAAAAGATTTCCTCA+GGG | 0.679415 | 4_1:+86740066 | Msa0581360:CDS |
| ATTCAGCTATCATCAAAAGG+AGG | 0.686014 | 4_1:+86739418 | Msa0581360:intron |
| CATTCTTGAGGAAGACCCTG+AGG | 0.700428 | 4_1:-86740081 | None:intergenic |
| TAAGAATGATTGGTTCTCCG+AGG | 0.717666 | 4_1:+86739972 | Msa0581360:CDS |
| CGTGCTAAAATACTCAACGG+TGG | 0.743251 | 4_1:+86739158 | Msa0581360:CDS |
| AAGACTTTGATATAACATGG+GGG | 0.749171 | 4_1:+86739129 | Msa0581360:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCAAATATACTTACATAAT+AGG | - | chr4_1:86739312-86739331 | None:intergenic | 15.0% |
| !!! | TTTATTTATTTTTTTGAGAG+AGG | - | chr4_1:86740246-86740265 | None:intergenic | 15.0% |
| !! | TTGTTATAGTTGAGAAATAT+GGG | - | chr4_1:86738976-86738995 | None:intergenic | 20.0% |
| !!! | TTTGTTATAGTTGAGAAATA+TGG | - | chr4_1:86738977-86738996 | None:intergenic | 20.0% |
| !!! | AGCTTTGATAACTAATTTTT+TGG | + | chr4_1:86739381-86739400 | Msa0581360:intron | 20.0% |
| !!! | AAGTTCAAATACTTTAAGAC+TGG | - | chr4_1:86739009-86739028 | None:intergenic | 25.0% |
| ! | ATCAAGACTTTGATATAACA+TGG | + | chr4_1:86739126-86739145 | Msa0581360:CDS | 25.0% |
| ! | TCAAGACTTTGATATAACAT+GGG | + | chr4_1:86739127-86739146 | Msa0581360:CDS | 25.0% |
| !! | TCCAAAAATGAATACCTTTT+TGG | + | chr4_1:86739230-86739249 | Msa0581360:CDS | 25.0% |
| ! | TAAAAGAACATTACTTGTGT+TGG | + | chr4_1:86739665-86739684 | Msa0581360:intron | 25.0% |
| ! | ATGTTCTTGAATTCTCTAAT+TGG | - | chr4_1:86739748-86739767 | None:intergenic | 25.0% |
| ! | AATTAGAGAATTCAAGAACA+TGG | + | chr4_1:86739747-86739766 | Msa0581360:CDS | 25.0% |
| ! | TACAATTATACAACATGTTG+AGG | - | chr4_1:86740141-86740160 | None:intergenic | 25.0% |
| ! | TGTGTAAGTGATCATTATAA+AGG | + | chr4_1:86740212-86740231 | Msa0581360:three_prime_UTR | 25.0% |
| !!! | TATTTTTTTGAGAGAGGAAT+TGG | - | chr4_1:86740240-86740259 | None:intergenic | 25.0% |
| TTGATAAAAATTGCCAGCAT+AGG | - | chr4_1:86739111-86739130 | None:intergenic | 30.0% | |
| CAAGACTTTGATATAACATG+GGG | + | chr4_1:86739128-86739147 | Msa0581360:CDS | 30.0% | |
| AAGACTTTGATATAACATGG+GGG | + | chr4_1:86739129-86739148 | Msa0581360:CDS | 30.0% | |
| CTCTCTCTTGATAAATATTC+TGG | + | chr4_1:86739194-86739213 | Msa0581360:CDS | 30.0% | |
| !!! | GCCAAAAAGGTATTCATTTT+TGG | - | chr4_1:86739234-86739253 | None:intergenic | 30.0% |
| ! | GCATATCAATTTTGCCAAAA+AGG | - | chr4_1:86739247-86739266 | None:intergenic | 30.0% |
| CTAATTCAGCTATCATCAAA+AGG | + | chr4_1:86739415-86739434 | Msa0581360:intron | 30.0% | |
| TGAGATAGACTTTGAATTCT+TGG | + | chr4_1:86739450-86739469 | Msa0581360:CDS | 30.0% | |
| GAGATAGACTTTGAATTCTT+GGG | + | chr4_1:86739451-86739470 | Msa0581360:CDS | 30.0% | |
| ATATTGGTATGAAGGATGTA+AGG | - | chr4_1:86739493-86739512 | None:intergenic | 30.0% | |
| CTGAGAAACTGAAAAATACA+TGG | - | chr4_1:86739714-86739733 | None:intergenic | 30.0% | |
| !! | TGTATTTTTCAGTTTCTCAG+TGG | + | chr4_1:86739714-86739733 | Msa0581360:intron | 30.0% |
| CACAATCTGATAAGAATGAT+TGG | + | chr4_1:86739962-86739981 | Msa0581360:CDS | 30.0% | |
| TGATAACAAAAGATTTCCTC+AGG | + | chr4_1:86740065-86740084 | Msa0581360:CDS | 30.0% | |
| GATAACAAAAGATTTCCTCA+GGG | + | chr4_1:86740066-86740085 | Msa0581360:CDS | 30.0% | |
| ATCTCCTTGTAAGTAGTCAA+TGG | + | chr4_1:86739031-86739050 | Msa0581360:exon | 35.0% | |
| CAGTCGAAGTAAAAATGACT+AGG | - | chr4_1:86739079-86739098 | None:intergenic | 35.0% | |
| AGACTTTGATATAACATGGG+GGG | + | chr4_1:86739130-86739149 | Msa0581360:CDS | 35.0% | |
| !! | CTTGATAAATATTCTGGCTC+TGG | + | chr4_1:86739200-86739219 | Msa0581360:CDS | 35.0% |
| ! | ATGCAACTTAAACTTGTTCC+TGG | + | chr4_1:86739263-86739282 | Msa0581360:CDS | 35.0% |
| ATTCAGCTATCATCAAAAGG+AGG | + | chr4_1:86739418-86739437 | Msa0581360:intron | 35.0% | |
| GAATTCTTGGGAAATTTGAG+TGG | + | chr4_1:86739463-86739482 | Msa0581360:CDS | 35.0% | |
| GGCTAAACATATTGGTATGA+AGG | - | chr4_1:86739501-86739520 | None:intergenic | 35.0% | |
| CATACCAATATGTTTAGCCA+AGG | + | chr4_1:86739502-86739521 | Msa0581360:CDS | 35.0% | |
| TTTGCCTTGGCTAAACATAT+TGG | - | chr4_1:86739509-86739528 | None:intergenic | 35.0% | |
| AATATGTTTAGCCAAGGCAA+AGG | + | chr4_1:86739508-86739527 | Msa0581360:CDS | 35.0% | |
| ATGTGTGAAAATCTGCAGTT+GGG | - | chr4_1:86739567-86739586 | None:intergenic | 35.0% | |
| TATGTGTGAAAATCTGCAGT+TGG | - | chr4_1:86739568-86739587 | None:intergenic | 35.0% | |
| TTCACACATACTCAATTCTG+TGG | + | chr4_1:86739578-86739597 | Msa0581360:CDS | 35.0% | |
| !! | TTTTTCAGTTTCTCAGTGGA+TGG | + | chr4_1:86739718-86739737 | Msa0581360:intron | 35.0% |
| TTCAAGAACATGGAATCAAG+TGG | + | chr4_1:86739757-86739776 | Msa0581360:CDS | 35.0% | |
| AGACTAGAATAGATCCTCAT+TGG | - | chr4_1:86739805-86739824 | None:intergenic | 35.0% | |
| ! | TGAGGATCTATTCTAGTCTT+TGG | + | chr4_1:86739806-86739825 | Msa0581360:CDS | 35.0% |
| !! | TCTTTGGAATGCTGATGATT+GGG | + | chr4_1:86739822-86739841 | Msa0581360:CDS | 35.0% |
| TTTCTGTATGAAGCAGTGAA+AGG | - | chr4_1:86739889-86739908 | None:intergenic | 35.0% | |
| !! | TCTTCTGGAATATCATCTTG+TGG | + | chr4_1:86739934-86739953 | Msa0581360:CDS | 35.0% |
| TAGATTCTACAAGTCTTGAG+AGG | + | chr4_1:86739998-86740017 | Msa0581360:CDS | 35.0% | |
| ! | AGTGCCATTGACTACTTACA+AGG | - | chr4_1:86739038-86739057 | None:intergenic | 40.0% |
| TTTGATATAACATGGGGGGA+TGG | + | chr4_1:86739134-86739153 | Msa0581360:CDS | 40.0% | |
| GGTCGTGCTAAAATACTCAA+CGG | + | chr4_1:86739155-86739174 | Msa0581360:CDS | 40.0% | |
| CAAGAGAGAGAGTAAGAAGT+TGG | - | chr4_1:86739184-86739203 | None:intergenic | 40.0% | |
| ! | TATCATCAAAAGGAGGTGCA+TGG | + | chr4_1:86739425-86739444 | Msa0581360:CDS | 40.0% |
| ! | ATCATCAAAAGGAGGTGCAT+GGG | + | chr4_1:86739426-86739445 | Msa0581360:CDS | 40.0% |
| GAGAGCAACAATTCTACCTT+TGG | + | chr4_1:86739536-86739555 | Msa0581360:CDS | 40.0% | |
| AGTACTTACACAATGCGTTG+TGG | - | chr4_1:86739607-86739626 | None:intergenic | 40.0% | |
| !!! | ATCCTCATTGGCTGGTTTTT+GGG | - | chr4_1:86739793-86739812 | None:intergenic | 40.0% |
| TAGAATAGATCCTCATTGGC+TGG | - | chr4_1:86739801-86739820 | None:intergenic | 40.0% | |
| !! | GTCTTTGGAATGCTGATGAT+TGG | + | chr4_1:86739821-86739840 | Msa0581360:CDS | 40.0% |
| !! | GAGGACTTGTTAAGACAGAT+TGG | + | chr4_1:86739854-86739873 | Msa0581360:CDS | 40.0% |
| ! | TAAGAATGATTGGTTCTCCG+AGG | + | chr4_1:86739972-86739991 | Msa0581360:CDS | 40.0% |
| ! | ACTTGTAGAATCTAGCTCCT+CGG | - | chr4_1:86739992-86740011 | None:intergenic | 40.0% |
| TAAGTCATCCTGCATTCTTG+AGG | - | chr4_1:86740096-86740115 | None:intergenic | 40.0% | |
| TCAAGAATGCAGGATGACTT+AGG | + | chr4_1:86740095-86740114 | Msa0581360:CDS | 40.0% | |
| CAAGAATGCAGGATGACTTA+GGG | + | chr4_1:86740096-86740115 | Msa0581360:CDS | 40.0% | |
| AAGAATGCAGGATGACTTAG+GGG | + | chr4_1:86740097-86740116 | Msa0581360:CDS | 40.0% | |
| ACTGTTGCAATTGCCTATGC+TGG | + | chr4_1:86739095-86739114 | Msa0581360:CDS | 45.0% | |
| CGTGCTAAAATACTCAACGG+TGG | + | chr4_1:86739158-86739177 | Msa0581360:CDS | 45.0% | |
| !!! | ATTGGCTGGTTTTTGGGGAA+TGG | - | chr4_1:86739787-86739806 | None:intergenic | 45.0% |
| !!! | TCCTCATTGGCTGGTTTTTG+GGG | - | chr4_1:86739792-86739811 | None:intergenic | 45.0% |
| !!! | GATCCTCATTGGCTGGTTTT+TGG | - | chr4_1:86739794-86739813 | None:intergenic | 45.0% |
| TGAAGCAGTGAAAGGAGCTT+TGG | - | chr4_1:86739881-86739900 | None:intergenic | 45.0% | |
| AATGCATGCACTGTGTCTTC+TGG | + | chr4_1:86739919-86739938 | Msa0581360:CDS | 45.0% | |
| AAGTCTTGAGAGGCTCAAGT+GGG | + | chr4_1:86740008-86740027 | Msa0581360:CDS | 45.0% | |
| CTGCAGTTGGGTCAAACCAA+AGG | - | chr4_1:86739555-86739574 | None:intergenic | 50.0% | |
| TCCCCAAAAACCAGCCAATG+AGG | + | chr4_1:86739788-86739807 | Msa0581360:CDS | 50.0% | |
| GCTGATGATTGGGCTACAAG+AGG | + | chr4_1:86739832-86739851 | Msa0581360:CDS | 50.0% | |
| GATGATTGGGCTACAAGAGG+AGG | + | chr4_1:86739835-86739854 | Msa0581360:CDS | 50.0% | |
| CAAGTCTTGAGAGGCTCAAG+TGG | + | chr4_1:86740007-86740026 | Msa0581360:CDS | 50.0% | |
| CATTCTTGAGGAAGACCCTG+AGG | - | chr4_1:86740084-86740103 | None:intergenic | 50.0% | |
| AGGGTCTTCCTCAAGAATGC+AGG | + | chr4_1:86740085-86740104 | Msa0581360:CDS | 50.0% | |
| CTTGTTCCTGGCAACTCTGC+TGG | + | chr4_1:86739275-86739294 | Msa0581360:CDS | 55.0% | |
| GCTCTCTGTCACCTTTGCCT+TGG | - | chr4_1:86739522-86739541 | None:intergenic | 55.0% | |
| ACAGTGCCAGCAGAGTTGCC+AGG | - | chr4_1:86739284-86739303 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 86738973 | 86740266 | 86738973 | ID=Msa0581360;Name=Msa0581360 |
| chr4_1 | mRNA | 86738973 | 86740266 | 86738973 | ID=Msa0581360-mRNA-1;Parent=Msa0581360;Name=Msa0581360-mRNA-1;_AED=0.06;_eAED=0.06;_QI=77|1|1|1|1|1|3|150|283 |
| chr4_1 | exon | 86738973 | 86739316 | 86738973 | ID=Msa0581360-mRNA-1:exon:32073;Parent=Msa0581360-mRNA-1 |
| chr4_1 | exon | 86739424 | 86739617 | 86739424 | ID=Msa0581360-mRNA-1:exon:32074;Parent=Msa0581360-mRNA-1 |
| chr4_1 | exon | 86739726 | 86740266 | 86739726 | ID=Msa0581360-mRNA-1:exon:32075;Parent=Msa0581360-mRNA-1 |
| chr4_1 | five_prime_UTR | 86738973 | 86739049 | 86738973 | ID=Msa0581360-mRNA-1:five_prime_utr;Parent=Msa0581360-mRNA-1 |
| chr4_1 | CDS | 86739050 | 86739316 | 86739050 | ID=Msa0581360-mRNA-1:cds;Parent=Msa0581360-mRNA-1 |
| chr4_1 | CDS | 86739424 | 86739617 | 86739424 | ID=Msa0581360-mRNA-1:cds;Parent=Msa0581360-mRNA-1 |
| chr4_1 | CDS | 86739726 | 86740116 | 86739726 | ID=Msa0581360-mRNA-1:cds;Parent=Msa0581360-mRNA-1 |
| chr4_1 | three_prime_UTR | 86740117 | 86740266 | 86740117 | ID=Msa0581360-mRNA-1:three_prime_utr;Parent=Msa0581360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
