AlfalfaGEDB | Msa1221280

M. sativa cultivar zhongmu-4 / Msa1221280

KEGG: K08235|K14504
GO: GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
PFAM: PF00722.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221280	sp\|Q38910\|XTH23_ARATH	74.205	283	70	2	1	280	1	283	2.56e-157	442

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221280	G7JVI0	95.406	283	13	0	1	283	1	283	0.0	561

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0266220	Msa1221280	0.810765	9.795591e-51	-8.615850e-47
Msa0581360	Msa1221280	0.944006	4.293967e-103	-8.615850e-47
Msa0641960	Msa1221280	0.806351	8.579331e-50	-8.615850e-47
Msa0680900	Msa1221280	0.802508	5.425635e-49	-8.615850e-47
Msa1221280	Msa1252000	0.801881	7.302506e-49	-8.615850e-47
Msa1221280	Msa1337440	0.894309	2.783468e-75	-8.615850e-47
Msa1221280	Msa1350700	0.804556	2.041486e-49	-8.615850e-47
Msa1221280	Msa1360260	0.805717	1.166810e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221280	MtrunA17_Chr4g0072611	95.406	283	13	0	1	283	1	283	0.0	561
Msa1221280	MtrunA17_Chr7g0258911	91.873	283	22	1	1	283	1	282	0.0	514
Msa1221280	MtrunA17_Chr4g0072671	85.171	263	35	2	22	280	23	285	2.97e-164	457
Msa1221280	MtrunA17_Chr4g0072601	74.021	281	69	1	4	280	3	283	3.59e-157	439
Msa1221280	MtrunA17_Chr4g0071341	75.185	270	66	1	14	282	14	283	9.48e-148	415
Msa1221280	MtrunA17_Chr2g0299061	70.397	277	77	1	9	280	10	286	3.15e-144	407
Msa1221280	MtrunA17_Chr8g0377841	72.059	272	71	1	9	280	15	281	8.47e-144	405
Msa1221280	MtrunA17_Chr3g0122031	70.819	281	75	2	1	281	5	278	8.14e-143	402
Msa1221280	MtrunA17_Chr3g0122021	70.819	281	75	2	1	281	5	278	9.92e-143	402
Msa1221280	MtrunA17_Chr4g0072681	74.486	243	54	2	46	280	1	243	2.93e-130	369

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221280	AT4G25810.1	74.205	283	70	2	1	280	1	283	2.60e-158	442
Msa1221280	AT5G57550.1	70.251	279	80	1	3	281	8	283	5.61e-151	424
Msa1221280	AT5G57560.1	70.175	285	76	3	1	280	1	281	6.40e-151	423
Msa1221280	AT4G30270.1	68.841	276	68	2	9	280	4	265	3.37e-144	406
Msa1221280	AT3G23730.1	66.431	283	91	1	3	281	5	287	8.81e-142	400
Msa1221280	AT4G14130.1	69.853	272	81	1	11	281	14	285	1.57e-141	400
Msa1221280	AT5G48070.1	64.855	276	90	3	8	280	10	281	2.07e-138	392
Msa1221280	AT5G57530.1	68.846	260	81	0	23	282	25	284	3.10e-136	386
Msa1221280	AT5G57540.1	67.050	261	86	0	22	282	23	283	2.02e-134	382
Msa1221280	AT4G25820.1	67.293	266	87	0	17	282	22	287	4.18e-133	379

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Find 70 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATGCAGGATGACTTAG+GGG	0.644048	8_1:+84847271	Msa1221280:CDS
AAGACTTTGATATAACATGG+GGG	0.749171	8_1:+84846303	Msa1221280:CDS
AAGTCTTGAGAGGCTCAAGT+GGG	0.675842	8_1:+84847182	Msa1221280:CDS
AAGTTCAAATACTTTAAGAT+TGG	0.409635	8_1:-84846180	None:intergenic
AATGCATGCACTGTGTCTTC+TGG	0.301674	8_1:+84847093	Msa1221280:CDS
AATGTGTTTAGCCAAGGCAA+AGG	0.660260	8_1:+84846682	Msa1221280:CDS
AATTAGAGAATTCAAGAACA+TGG	0.587989	8_1:+84846921	Msa1221280:CDS
ACAGTGCCAGCAGAGTTGCC+AGG	0.543526	8_1:-84846455	None:intergenic
ACATTGGTATGAAGGATGTA+AGG	0.521423	8_1:-84846664	None:intergenic
ACTGTTGCAATTGCCTATGC+TGG	0.488876	8_1:+84846269	Msa1221280:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGATAAAAATTGCCAGCAT+AGG	-	chr8_1:84846285-84846304	None:intergenic	30.0%
CAAGACTTTGATATAACATG+GGG	+	chr8_1:84846302-84846321	Msa1221280:CDS	30.0%
AAGACTTTGATATAACATGG+GGG	+	chr8_1:84846303-84846322	Msa1221280:CDS	30.0%
CTCTCTCTTGATAAATATTC+TGG	+	chr8_1:84846368-84846387	Msa1221280:CDS	30.0%
CTAATTCAGCTATCATCAAA+AGG	+	chr8_1:84846589-84846608	Msa1221280:intron	30.0%
TGAGATAGACTTTGAATTCT+TGG	+	chr8_1:84846624-84846643	Msa1221280:CDS	30.0%
GAGATAGACTTTGAATTCTT+GGG	+	chr8_1:84846625-84846644	Msa1221280:CDS	30.0%
CTGAGAAACTGAAAAATACA+TGG	-	chr8_1:84846888-84846907	None:intergenic	30.0%
CACAATCTGATAAGAATGAT+TGG	+	chr8_1:84847136-84847155	Msa1221280:CDS	30.0%
TGATAACAAAAGATTTCCTC+AGG	+	chr8_1:84847239-84847258	Msa1221280:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	84846147	84847440	84846147	ID=Msa1221280;Name=Msa1221280
chr8_1	mRNA	84846147	84847440	84846147	ID=Msa1221280-mRNA-1;Parent=Msa1221280;Name=Msa1221280-mRNA-1;_AED=0.40;_eAED=0.40;_QI=77\|1\|1\|1\|1\|1\|3\|150\|283
chr8_1	exon	84846147	84846490	84846147	ID=Msa1221280-mRNA-1:exon:30468;Parent=Msa1221280-mRNA-1
chr8_1	exon	84846598	84846791	84846598	ID=Msa1221280-mRNA-1:exon:30469;Parent=Msa1221280-mRNA-1
chr8_1	exon	84846900	84847440	84846900	ID=Msa1221280-mRNA-1:exon:30470;Parent=Msa1221280-mRNA-1
chr8_1	five_prime_UTR	84846147	84846223	84846147	ID=Msa1221280-mRNA-1:five_prime_utr;Parent=Msa1221280-mRNA-1
chr8_1	CDS	84846224	84846490	84846224	ID=Msa1221280-mRNA-1:cds;Parent=Msa1221280-mRNA-1
chr8_1	CDS	84846598	84846791	84846598	ID=Msa1221280-mRNA-1:cds;Parent=Msa1221280-mRNA-1
chr8_1	CDS	84846900	84847290	84846900	ID=Msa1221280-mRNA-1:cds;Parent=Msa1221280-mRNA-1
chr8_1	three_prime_UTR	84847291	84847440	84847291	ID=Msa1221280-mRNA-1:three_prime_utr;Parent=Msa1221280-mRNA-1

Gene Sequence

>Msa1221280

ATGGCACTTTCAAAATTTGTCACATTCCTAGTCATTTTTACTTCGACTGTTGCAATTGCCTATGCTGGCAATTTTTATCAAGACTTTGATATAACATGGGGGGATGGTCGTGCTAAAATACTCAACGGTGGCCAACTTCTTACTCTCTCTCTTGATAAATATTCTGGCTCTGGCTTTCAATCCAAAAATGAATACCTTTTTGGCAAAATTGATATGCAACTTAAACTTGTTCCTGGCAACTCTGCTGGCACTGTCACTGCCTATTATCTATCATCAAAAGGAGGTGCATGGGATGAGATAGACTTTGAATTCTTGGGAAATTTGAGTGGTGATCCTTACATCCTTCATACCAATGTGTTTAGCCAAGGCAAAGGTGACAGAGAGCAACAATTCTACCTTTGGTTTGACCCAACTGCAGATTTTCACACATACTCAATTCTGTGGAATCCACAACGCATTGTTTTCTCAGTGGATGGAACTCCAATTAGAGAATTCAAGAACATGGAATCAAGTGGTGTTCCATTCCCCAAAAACCAGCCAATGAGGATCTATTCTAGTCTTTGGAATGCTGATGATTGGGCTACAAGAGGAGGACTTGTTAAGACAGATTGGTCCAAAGCTCCTTTCACTGCTTCATACAGAAATTTCAATGCTAATGCATGCACTGTGTCTTCTGGAATATCATCTTGTGGTTCATCACAATCTGATAAGAATGATTGGTTCTCCGAGGAGCTAGATTCTACAAGTCTTGAGAGGCTCAAGTGGGTTCAGAAGAATTACATGATCTATAATTACTGCAGTGATAACAAAAGATTTCCTCAGGGTCTTCCTCAAGAATGCAGGATGACTTAG

Protein sequence

>Msa1221280

MALSKFVTFLVIFTSTVAIAYAGNFYQDFDITWGDGRAKILNGGQLLTLSLDKYSGSGFQSKNEYLFGKIDMQLKLVPGNSAGTVTAYYLSSKGGAWDEIDFEFLGNLSGDPYILHTNVFSQGKGDREQQFYLWFDPTADFHTYSILWNPQRIVFSVDGTPIREFKNMESSGVPFPKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNANACTVSSGISSCGSSQSDKNDWFSEELDSTSLERLKWVQKNYMIYNYCSDNKRFPQGLPQECRMT*