AlfalfaGEDB | Msa0585280

M. sativa cultivar zhongmu-4 / Msa0585280

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0585280	GAU50549.1	74.167	480	103	4	1	475	1	464	0.0	705

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0585280	sp\|Q9SZL8\|FRS5_ARATH	40.493	284	160	2	111	390	411	689	1.67e-62	223

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0585280	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0585280	Msa0595880	0.902822	6.544699e-79	-8.615850e-47
Msa0585280	Msa0602590	0.837078	6.426949e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0585280	MtrunA17_Chr1g0202341	77.104	297	68	0	114	410	389	685	3.76e-164	484
Msa0585280	MtrunA17_Chr1g0202341	63.717	113	39	1	4	114	12	124	4.39e-37	146
Msa0585280	MtrunA17_Chr5g0448761	42.069	290	155	2	114	397	405	687	2.48e-71	244
Msa0585280	MtrunA17_Chr5g0448511	37.979	287	171	2	114	397	177	459	1.16e-67	229
Msa0585280	MtrunA17_Chr3g0118611	31.387	411	238	8	115	504	284	671	4.44e-57	205
Msa0585280	MtrunA17_Chr5g0440451	37.024	289	165	4	115	397	387	664	2.92e-55	201
Msa0585280	MtrunA17_Chr3g0080351	35.125	279	170	3	114	390	404	673	2.89e-48	180
Msa0585280	MtrunA17_Chr1g0198761	30.641	359	229	6	123	480	405	744	1.04e-45	172
Msa0585280	MtrunA17_Chr2g0287061	35.811	296	172	5	105	394	474	757	2.09e-44	169
Msa0585280	MtrunA17_Chr2g0329031	30.086	349	224	6	113	449	342	682	2.07e-43	166

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0585280	AT4G38180.1	40.493	284	160	2	111	390	411	689	1.70e-63	223
Msa0585280	AT4G38170.1	32.941	340	215	5	115	452	180	508	8.79e-57	200
Msa0585280	AT2G27110.3	36.071	280	173	2	115	394	217	490	2.20e-54	197
Msa0585280	AT2G27110.2	36.071	280	173	2	115	394	362	635	8.98e-54	197
Msa0585280	AT2G27110.1	36.071	280	173	2	115	394	362	635	8.98e-54	197
Msa0585280	AT5G18960.2	35.374	294	165	4	110	394	507	784	2.58e-49	183
Msa0585280	AT5G18960.1	35.374	294	165	4	110	394	507	784	2.58e-49	183
Msa0585280	AT3G06250.2	33.333	294	171	4	110	394	483	760	1.53e-47	178
Msa0585280	AT3G06250.1	33.333	294	171	4	110	394	483	760	1.53e-47	178
Msa0585280	AT3G06250.3	33.333	294	171	4	110	394	483	760	1.53e-47	178

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Find 122 sgRNAs with CRISPR-Local

Find 349 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGTTCAAGATATTCC+AGG	0.469314	4_2:-5849313	Msa0585280:CDS
AAAGTACGTGGACGACCTTT+AGG	0.294239	4_2:+5848730	None:intergenic
AAATTGTGACGTACATAAGA+AGG	0.554526	4_2:+5849561	None:intergenic
AAGTACGTGGACGACCTTTA+GGG	0.355782	4_2:+5848731	None:intergenic
AATACTACACAAGGTACGCG+AGG	0.651596	4_2:-5850664	Msa0585280:CDS
AATCACAAAACTTGATCAAT+TGG	0.417160	4_2:-5848857	Msa0585280:CDS
AATGAAGTGTAGTTATTGCC+AGG	0.439936	4_2:-5848668	Msa0585280:CDS
AATGGCAGAAAATCAGCACC+AGG	0.625686	4_2:+5847024	None:intergenic
AATGGTAGTCTATCATTGAG+AGG	0.621900	4_2:+5850746	None:intergenic
AATTTCATGAATCTTGTATG+TGG	0.536832	4_2:+5849230	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAACCACATCTTCAATTACT+TGG	-	chr4_2:5845992-5846011	Msa0585280:intron	30.0%
AAACCAAGTAATTGAAGATG+TGG	+	chr4_2:5845998-5846017	None:intergenic	30.0%
TCAAGAGATTCTTTGTTCAA+AGG	-	chr4_2:5846496-5846515	Msa0585280:intron	30.0%
CGAATCATCATTATTGTATG+TGG	-	chr4_2:5847287-5847306	Msa0585280:intron	30.0%
CACTATGTTCATGATAGATT+TGG	+	chr4_2:5847345-5847364	None:intergenic	30.0%
TGATGACTTTGATTCTGATT+GGG	-	chr4_2:5847411-5847430	Msa0585280:intron	30.0%
AAATTGTGACGTACATAAGA+AGG	+	chr4_2:5847514-5847533	None:intergenic	30.0%
ATTCATACTTTCACTCCTTT+GGG	+	chr4_2:5847557-5847576	None:intergenic	30.0%
TATTCATACTTTCACTCCTT+TGG	+	chr4_2:5847558-5847577	None:intergenic	30.0%
GAGAGAAGAATGTTAAAACA+AGG	-	chr4_2:5847671-5847690	Msa0585280:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	5845774	5851320	5845774	ID=Msa0585280;Name=Msa0585280
chr4_2	mRNA	5845774	5851320	5845774	ID=Msa0585280-mRNA-1;Parent=Msa0585280;Name=Msa0585280-mRNA-1;_AED=0.20;_eAED=0.37;_QI=236\|0.33\|0.14\|0.71\|0.66\|0.71\|7\|0\|591
chr4_2	exon	5845774	5845893	5845774	ID=Msa0585280-mRNA-1:exon:1446;Parent=Msa0585280-mRNA-1
chr4_2	exon	5847008	5847110	5847008	ID=Msa0585280-mRNA-1:exon:1445;Parent=Msa0585280-mRNA-1
chr4_2	exon	5848261	5848341	5848261	ID=Msa0585280-mRNA-1:exon:1444;Parent=Msa0585280-mRNA-1
chr4_2	exon	5848457	5848501	5848457	ID=Msa0585280-mRNA-1:exon:1443;Parent=Msa0585280-mRNA-1
chr4_2	exon	5848622	5849706	5848622	ID=Msa0585280-mRNA-1:exon:1442;Parent=Msa0585280-mRNA-1
chr4_2	exon	5850508	5850873	5850508	ID=Msa0585280-mRNA-1:exon:1441;Parent=Msa0585280-mRNA-1
chr4_2	exon	5851109	5851320	5851109	ID=Msa0585280-mRNA-1:exon:1440;Parent=Msa0585280-mRNA-1
chr4_2	five_prime_UTR	5851109	5851320	5851109	ID=Msa0585280-mRNA-1:five_prime_utr;Parent=Msa0585280-mRNA-1
chr4_2	five_prime_UTR	5850850	5850873	5850850	ID=Msa0585280-mRNA-1:five_prime_utr;Parent=Msa0585280-mRNA-1
chr4_2	CDS	5850508	5850849	5850508	ID=Msa0585280-mRNA-1:cds;Parent=Msa0585280-mRNA-1
chr4_2	CDS	5848622	5849706	5848622	ID=Msa0585280-mRNA-1:cds;Parent=Msa0585280-mRNA-1
chr4_2	CDS	5848457	5848501	5848457	ID=Msa0585280-mRNA-1:cds;Parent=Msa0585280-mRNA-1
chr4_2	CDS	5848261	5848341	5848261	ID=Msa0585280-mRNA-1:cds;Parent=Msa0585280-mRNA-1
chr4_2	CDS	5847008	5847110	5847008	ID=Msa0585280-mRNA-1:cds;Parent=Msa0585280-mRNA-1
chr4_2	CDS	5845774	5845893	5845774	ID=Msa0585280-mRNA-1:cds;Parent=Msa0585280-mRNA-1

Gene Sequence

>Msa0585280

ATGACAGATCAAGAAATGACAGATATGCATGAACATACCAACATGATTGGCTCAGATGATATGCTTGCAAGTGATGCAGATCCTCTCAATGATAGACTACCATTGGAGCTCCCCTTAGAGCCCTTTGAAGGAATGGAGTTTACATCAATTGAAGATGTAAAAAAATACTACACAAGGTACGCGAGGAATAAAGGTTTTAGCTTTCGTATGGGTCGTGTTACTAAATCAAGAACGGATGGTAGGGTAATTGGTCAAGAGATTCTTTGTTCAAAGGAGGGGTTTAGATCCCAAAAAAATTTGAAAAAAGAAAATGGTAGTCTTATAGTACATGATCAAACAAGGTGCATTCACCAAACTATCACTACTGATGACTTTGATTCTGATTGGGGAGCAATGATAGATAAGTACGAGTTGCAAGATAACAAATGGCTAAAGAAGATCTACTCCATTAGAGCAAAGTGGATTCCTTCTTATGTACGTCACAATTTTTGTGCTGGAATGTCCACCACCCAAAGGAGTGAAAGTATGAATAAATTTTTTAAGGGCTTTTTGAATTCAAGTACTCCATTGAGCAAGTTTGTGACACAATATGAGAAGGCTCTTGATGCACGTTATAACAAGGAAAGAGAGAAGAATGTTAAAACAAGGAATTCAAAACCACTTTTGCGCACTTTATATCCTATGGAAGAAGAGGCTTCAAAAATTTATACACGAAAAGTGTTCAAGATATTCCAGGATGAGTTGGTTGGATCCCAAATATTTATCGCAGAGAAAGTTGAATTTTCTGTTGAAGTTTCCACATACAAGATTCATGAAATTTACAAAGAGAAACCTAACTATCATGTGACTTTTCATGACATTTCAAAAGAAGCAAAATGTAGTTGTCACATGTTTGAATTTGTGGGTATTCTTTGTAGACATGTGTTGAATGTATTCATCAAGGAAAATGTTTATTCTCTCCCTTCTCAATATGTTCTAAGTCGATGGACCATAAATGCTAAGAAAGACAAAAGTAAAGGACTTGCAATTGAAGACCTTGAAGAAGGCAGAAACCGAGCTTCAAGTACTTCATTGTTTAATAGTATTATGGTTGAGTCTCTTGAACTATCTGAGAGAGGTTCACGATCGCAAAAACATCATGATATTGCAATTCAAGCTTTGCGGAAGGCAATCACAAAACTTGATCAATTGGATATTGAAGAGCCAAATGAAGAGTTTGTTAATTCAACGTTTGAAGTTATGCCTAAAGTCTCCGATGACCAAACCATAAATTTGTGTGATCCAGCACGTGCTGTCCCTAAAGGTCGTCCACGTACTTTACGAATGAAAGGCAGTTTGGAATTGCTCAAAAAAGGTTCAATGAAGTGTAGTTATTGCCAGGGAAAAGGCCATAACAAACGTAGTTGTCCAAGTTTAAAACATGGAAGCGTTCAAATTCCAGTGTTAAAGCTTCTAGTTTGGTTGATTAAGAGACCTGCTTTGCCACTAAAGGATAGTTTTGTCGAACTCAAAGGTGGTGGGTTTCTCTGGTGTGATTTCAGATTTTGGATATGGTCTGGTGGCCGCTGCTCTGCTGTTTGTGTTGAAGCTGCAGGAAGGTTGTTTTTTAATCTGAAGCCTGGTGCTGATTTTCTGCCATTCGCTGCTAAAGGATTTGAGAACTACACTGAGGTACCTCCAAGTATTGCATCAGTATGCAGCAGTGTTCATGAAGACTTTTGGAGGCCAACTGAATATTTAATTCCAATATCAACTCCTGATTTGTCCTCGAGATGA

Protein sequence

>Msa0585280

MTDQEMTDMHEHTNMIGSDDMLASDADPLNDRLPLELPLEPFEGMEFTSIEDVKKYYTRYARNKGFSFRMGRVTKSRTDGRVIGQEILCSKEGFRSQKNLKKENGSLIVHDQTRCIHQTITTDDFDSDWGAMIDKYELQDNKWLKKIYSIRAKWIPSYVRHNFCAGMSTTQRSESMNKFFKGFLNSSTPLSKFVTQYEKALDARYNKEREKNVKTRNSKPLLRTLYPMEEEASKIYTRKVFKIFQDELVGSQIFIAEKVEFSVEVSTYKIHEIYKEKPNYHVTFHDISKEAKCSCHMFEFVGILCRHVLNVFIKENVYSLPSQYVLSRWTINAKKDKSKGLAIEDLEEGRNRASSTSLFNSIMVESLELSERGSRSQKHHDIAIQALRKAITKLDQLDIEEPNEEFVNSTFEVMPKVSDDQTINLCDPARAVPKGRPRTLRMKGSLELLKKGSMKCSYCQGKGHNKRSCPSLKHGSVQIPVLKLLVWLIKRPALPLKDSFVELKGGGFLWCDFRFWIWSGGRCSAVCVEAAGRLFFNLKPGADFLPFAAKGFENYTEVPPSIASVCSSVHEDFWRPTEYLIPISTPDLSSR*