AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0585970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585970 MtrunA17_Chr4g0001611 89.571 163 10 3 1 158 1 161 7.69e-93 266
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585970 AT1G80920.1 62.727 110 40 1 48 157 54 162 8.48e-48 152
Msa0585970 AT5G59610.1 29.323 133 78 3 24 146 54 180 8.68e-12 61.6
Msa0585970 AT5G59610.4 29.323 133 78 3 24 146 54 180 8.68e-12 61.6

Find 66 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTAACCAAACCTATTC+TGG 0.118254 4_2:-7080715 Msa0585970:five_prime_UTR
GCTACTGCTGTTGGTGTTAT+TGG 0.162018 4_2:-7080469 Msa0585970:CDS
TAATTGAAGTTGAGGATATT+TGG 0.195096 4_2:-7079221 Msa0585970:three_prime_UTR
CAAAGAGCACCAACCAAATT+TGG 0.237220 4_2:+7079284 None:intergenic
TGAATTCTTAAAGTCTTATA+AGG 0.256828 4_2:+7080331 None:intergenic
GGAATTGTATTTATAGAAAT+AGG 0.259428 4_2:+7080947 None:intergenic
GTGTTAAATATTTCAAAGTT+TGG 0.284170 4_2:-7080617 Msa0585970:five_prime_UTR
ATGATCCAGATTGGGGCTAT+TGG 0.284504 4_2:-7079454 Msa0585970:CDS
AATAAAACCACCTCAAAGTT+TGG 0.298635 4_2:-7080740 Msa0585970:five_prime_UTR
AAAATAAAATGAATAAAGTT+AGG 0.300912 4_2:-7080393 Msa0585970:CDS
AATCATTATTTAATAGTGTT+TGG 0.302164 4_2:-7080838 Msa0585970:five_prime_UTR
TATGTTCTTATCTTAAAGTT+TGG 0.324007 4_2:-7080679 Msa0585970:five_prime_UTR
CTTGTTTATAAGATTCCATT+TGG 0.340253 4_2:+7079123 None:intergenic
TCTCTTCCTTTGCACACATC+TGG 0.358215 4_2:+7080122 None:intergenic
TTAGTTATTATGTGTGAAAT+CGG 0.370612 4_2:+7080994 None:intergenic
TGATCCAGATTGGGGCTATT+GGG 0.375755 4_2:-7079453 Msa0585970:CDS
GTTGGTGTTATTGGTGGTAA+TGG 0.380315 4_2:-7080460 Msa0585970:CDS
GTAATGGATCTTGTGTTTCA+TGG 0.383277 4_2:-7080444 Msa0585970:CDS
GACAATGATGAGTCATTTAG+AGG 0.388181 4_2:-7079482 Msa0585970:CDS
GAGATATGACATACATTCAT+TGG 0.391558 4_2:+7079251 None:intergenic
TCTTCCCAATAGCCCCAATC+TGG 0.418268 4_2:+7079449 None:intergenic
TTTGTAACCAAACCTATTCT+GGG 0.422263 4_2:-7080714 Msa0585970:five_prime_UTR
GGGCTATTGGGAAGAATGGA+TGG 0.423463 4_2:-7079441 Msa0585970:CDS
GAGGAATGAATGATCCAGAT+TGG 0.426354 4_2:-7079463 Msa0585970:CDS
ACAATGACCCAGAATAGGTT+TGG 0.430276 4_2:+7080707 None:intergenic
AGGAATGAATGATCCAGATT+GGG 0.438090 4_2:-7079462 Msa0585970:CDS
TGTGTTTCATGGATGCAATC+TGG 0.438112 4_2:-7080433 Msa0585970:CDS
AATTCACAATGACCCAGAAT+AGG 0.446531 4_2:+7080702 None:intergenic
ATCTAATTCTTCTTCTGTGA+TGG 0.457322 4_2:-7080356 Msa0585970:CDS
ATCATTGTCTTCATTATATG+TGG 0.460660 4_2:+7079496 None:intergenic
ATTGGGGCTATTGGGAAGAA+TGG 0.464008 4_2:-7079445 Msa0585970:CDS
GATTGAAGAGATTGAAGAAA+GGG 0.466801 4_2:+7080926 None:intergenic
AAATAAAATGAATAAAGTTA+GGG 0.472329 4_2:-7080392 Msa0585970:CDS
TTCTGAATCTGATGTTAGAA+AGG 0.473384 4_2:-7080299 Msa0585970:CDS
TTGGGAAGAATGGATGGGTT+GGG 0.486704 4_2:-7079435 Msa0585970:CDS
ATGGATGCAATCTGGAAGAA+AGG 0.497841 4_2:-7080425 Msa0585970:CDS
ATTGGGAAGAATGGATGGGT+TGG 0.503999 4_2:-7079436 Msa0585970:CDS
TTCCTTTGCACACATCTGGA+TGG 0.515608 4_2:+7080126 None:intergenic
TTACAAAGAACCAAACTTTG+AGG 0.519100 4_2:+7080730 None:intergenic
GTCACGTTTGCATCAATCAA+TGG 0.519392 4_2:-7081024 Msa0585970:five_prime_UTR
ACTGCTGTTGGTGTTATTGG+TGG 0.523533 4_2:-7080466 Msa0585970:CDS
ATGGCTGCTGCTACTGCTGT+TGG 0.524618 4_2:-7080478 Msa0585970:CDS
GGCTATTGGGAAGAATGGAT+GGG 0.528910 4_2:-7079440 Msa0585970:CDS
GTGTGCAAAGGAAGAGATTG+TGG 0.529500 4_2:-7080116 Msa0585970:CDS
GAGCACCAACCAAATTTGGA+TGG 0.537248 4_2:+7079288 None:intergenic
CCTCAACATAATTGAAGTTG+AGG 0.538944 4_2:-7079229 Msa0585970:three_prime_UTR
TGATTGAAGAGATTGAAGAA+AGG 0.539837 4_2:+7080925 None:intergenic
AGCACCAACCAAATTTGGAT+GGG 0.548146 4_2:+7079289 None:intergenic
GAAGAATGGATGGGTTGGGA+AGG 0.548816 4_2:-7079431 Msa0585970:CDS
TGGATGGGTTGGGAAGGAGC+AGG 0.559751 4_2:-7079425 Msa0585970:CDS
CCTCAACTTCAATTATGTTG+AGG 0.569740 4_2:+7079229 None:intergenic
TGTGCAAAGGAAGAGATTGT+GGG 0.575497 4_2:-7080115 Msa0585970:CDS
AATGGAATCTTATAAACAAG+AGG 0.587710 4_2:-7079120 Msa0585970:three_prime_UTR
GCACCAACCAAATTTGGATG+GGG 0.605222 4_2:+7079290 None:intergenic
CAAAGAACCAAACTTTGAGG+TGG 0.605813 4_2:+7080733 None:intergenic
GGTTGAAGAACAAAATAGAG+TGG 0.607277 4_2:+7080880 None:intergenic
GCAGTTTCACTTGATCAATG+AGG 0.607876 4_2:-7080090 Msa0585970:CDS
TAATATGAGAAGAGTAGTCA+CGG 0.620194 4_2:+7079399 None:intergenic
TACCATCCAGATGTGTGCAA+AGG 0.629057 4_2:-7080128 Msa0585970:CDS
TAGAATTGGAACAATGGGCA+CGG 0.630287 4_2:+7080783 None:intergenic
GGAATGAATGATCCAGATTG+GGG 0.639140 4_2:-7079461 Msa0585970:CDS
TGATTGATGCAAACGTGACG+TGG 0.641491 4_2:+7081028 None:intergenic
ACATCAGATTCAGAAGCACC+AGG 0.654245 4_2:+7080307 None:intergenic
CACCAACCAAATTTGGATGG+GGG 0.668622 4_2:+7079291 None:intergenic
AAGACTTTAAGAATTCAACC+TGG 0.672860 4_2:-7080325 Msa0585970:CDS
GTGCAAAGGAAGAGATTGTG+GGG 0.740567 4_2:-7080114 Msa0585970:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAACAAAAAAAAAAATTC+TGG - chr4_2:7079565-7079584 Msa0585970:intron 10.0%
!! TTAACAAAAAAAAAAATTCT+GGG - chr4_2:7079566-7079585 Msa0585970:intron 10.0%
!! AAAATAAAATGAATAAAGTT+AGG - chr4_2:7079761-7079780 Msa0585970:intron 10.0%
!! AAATAAAATGAATAAAGTTA+GGG - chr4_2:7079762-7079781 Msa0585970:intron 10.0%
!! ATTCAATTATTATAATTGTT+TGG - chr4_2:7080159-7080178 Msa0585970:intron 10.0%
!! TTCAATTATTATAATTGTTT+GGG - chr4_2:7080160-7080179 Msa0585970:intron 10.0%
!! TTTAACAAAAAAAAAAATTC+TGG - chr4_2:7079565-7079584 Msa0585970:intron 10.0%
!! TTAACAAAAAAAAAAATTCT+GGG - chr4_2:7079566-7079585 Msa0585970:intron 10.0%
!! AAAATAAAATGAATAAAGTT+AGG - chr4_2:7079761-7079780 Msa0585970:intron 10.0%
!! AAATAAAATGAATAAAGTTA+GGG - chr4_2:7079762-7079781 Msa0585970:intron 10.0%
!! ATTCAATTATTATAATTGTT+TGG - chr4_2:7080159-7080178 Msa0585970:intron 10.0%
!! TTCAATTATTATAATTGTTT+GGG - chr4_2:7080160-7080179 Msa0585970:intron 10.0%
!! AATCATTATTTAATAGTGTT+TGG - chr4_2:7079316-7079335 Msa0585970:three_prime_UTR 15.0%
!!! TTTATAACAAAGAGTTTTTT+GGG - chr4_2:7079628-7079647 Msa0585970:intron 15.0%
!! TTGTACTAATATTGAATATT+TGG - chr4_2:7079982-7080001 Msa0585970:intron 15.0%
!! TTCAATCCTTAAATAAATAT+AGG - chr4_2:7080196-7080215 Msa0585970:intron 15.0%
!! CAATATCCTATATTTATTTA+AGG + chr4_2:7080205-7080224 None:intergenic 15.0%
!!! TTTTTTATTGTTTATGGTTT+TGG - chr4_2:7080364-7080383 Msa0585970:CDS 15.0%
!!! AATTTGTTTTTATCTTCTTA+TGG - chr4_2:7080497-7080516 Msa0585970:exon 15.0%
!!! ATTTGTTTTTATCTTCTTAT+GGG - chr4_2:7080498-7080517 Msa0585970:exon 15.0%
!! CATAAATTTCAATTAGATAA+TGG + chr4_2:7080565-7080584 None:intergenic 15.0%
!!! CATAAACTTTTTTAAATGTA+AGG + chr4_2:7080783-7080802 None:intergenic 15.0%
!! AATCATTATTTAATAGTGTT+TGG - chr4_2:7079316-7079335 Msa0585970:three_prime_UTR 15.0%
!!! TTTATAACAAAGAGTTTTTT+GGG - chr4_2:7079628-7079647 Msa0585970:intron 15.0%
!! TTGTACTAATATTGAATATT+TGG - chr4_2:7079982-7080001 Msa0585970:intron 15.0%
!! TTCAATCCTTAAATAAATAT+AGG - chr4_2:7080196-7080215 Msa0585970:intron 15.0%
!! CAATATCCTATATTTATTTA+AGG + chr4_2:7080205-7080224 None:intergenic 15.0%
!!! TTTTTTATTGTTTATGGTTT+TGG - chr4_2:7080364-7080383 Msa0585970:CDS 15.0%
!!! AATTTGTTTTTATCTTCTTA+TGG - chr4_2:7080497-7080516 Msa0585970:exon 15.0%
!!! ATTTGTTTTTATCTTCTTAT+GGG - chr4_2:7080498-7080517 Msa0585970:exon 15.0%
!! CATAAATTTCAATTAGATAA+TGG + chr4_2:7080565-7080584 None:intergenic 15.0%
!!! CATAAACTTTTTTAAATGTA+AGG + chr4_2:7080783-7080802 None:intergenic 15.0%
!! TTAGTTATTATGTGTGAAAT+CGG + chr4_2:7079163-7079182 None:intergenic 20.0%
!! GGAATTGTATTTATAGAAAT+AGG + chr4_2:7079210-7079229 None:intergenic 20.0%
!!! AAGTTGATTTTGATTTTGAT+GGG + chr4_2:7079350-7079369 None:intergenic 20.0%
!!! AGAAGATATTGTTTTAGAAT+TGG + chr4_2:7079388-7079407 None:intergenic 20.0%
!! TATGTTCTTATCTTAAAGTT+TGG - chr4_2:7079475-7079494 Msa0585970:CDS 20.0%
!! GTGTTAAATATTTCAAAGTT+TGG - chr4_2:7079537-7079556 Msa0585970:CDS 20.0%
!! GAGTTAGATATAAAAAAGTT+TGG - chr4_2:7079602-7079621 Msa0585970:intron 20.0%
!!! CTTTATAACAAAGAGTTTTT+TGG - chr4_2:7079627-7079646 Msa0585970:intron 20.0%
!!! AGAATTTGTGATTTTAAGAA+TGG - chr4_2:7079657-7079676 Msa0585970:intron 20.0%
!!! TGAATTCTTAAAGTCTTATA+AGG + chr4_2:7079826-7079845 None:intergenic 20.0%
!!! TCTAATTTGATTGATGATTT+CGG - chr4_2:7079927-7079946 Msa0585970:intron 20.0%
!! TAACTATGCAATGAAAATTA+AGG + chr4_2:7080128-7080147 None:intergenic 20.0%
!!! TATAGGATATTGATTGATAT+TGG - chr4_2:7080213-7080232 Msa0585970:intron 20.0%
!! ACAATAAAAAAAACTGGATT+TGG + chr4_2:7080355-7080374 None:intergenic 20.0%
!! CCATAAACAATAAAAAAAAC+TGG + chr4_2:7080361-7080380 None:intergenic 20.0%
!!! CCAGTTTTTTTTATTGTTTA+TGG - chr4_2:7080358-7080377 Msa0585970:CDS 20.0%
!! GTTTATCAAGTTATAAATCT+TGG + chr4_2:7080401-7080420 None:intergenic 20.0%
!! TCAAACAAATCATTAAACTA+TGG + chr4_2:7080525-7080544 None:intergenic 20.0%
!!! AATTTATGTGTTGCTTATAT+TGG - chr4_2:7080577-7080596 Msa0585970:five_prime_UTR 20.0%
!!! TTTTCTAATCTTTATCCAAA+TGG - chr4_2:7081016-7081035 Msa0585970:five_prime_UTR 20.0%
!! TTAGTTATTATGTGTGAAAT+CGG + chr4_2:7079163-7079182 None:intergenic 20.0%
!! GGAATTGTATTTATAGAAAT+AGG + chr4_2:7079210-7079229 None:intergenic 20.0%
!!! AAGTTGATTTTGATTTTGAT+GGG + chr4_2:7079350-7079369 None:intergenic 20.0%
!!! AGAAGATATTGTTTTAGAAT+TGG + chr4_2:7079388-7079407 None:intergenic 20.0%
!! TATGTTCTTATCTTAAAGTT+TGG - chr4_2:7079475-7079494 Msa0585970:CDS 20.0%
!! GTGTTAAATATTTCAAAGTT+TGG - chr4_2:7079537-7079556 Msa0585970:CDS 20.0%
!! GAGTTAGATATAAAAAAGTT+TGG - chr4_2:7079602-7079621 Msa0585970:intron 20.0%
!!! CTTTATAACAAAGAGTTTTT+TGG - chr4_2:7079627-7079646 Msa0585970:intron 20.0%
!!! AGAATTTGTGATTTTAAGAA+TGG - chr4_2:7079657-7079676 Msa0585970:intron 20.0%
!!! TGAATTCTTAAAGTCTTATA+AGG + chr4_2:7079826-7079845 None:intergenic 20.0%
!!! TCTAATTTGATTGATGATTT+CGG - chr4_2:7079927-7079946 Msa0585970:intron 20.0%
!! TAACTATGCAATGAAAATTA+AGG + chr4_2:7080128-7080147 None:intergenic 20.0%
!!! TATAGGATATTGATTGATAT+TGG - chr4_2:7080213-7080232 Msa0585970:intron 20.0%
!! ACAATAAAAAAAACTGGATT+TGG + chr4_2:7080355-7080374 None:intergenic 20.0%
!! CCATAAACAATAAAAAAAAC+TGG + chr4_2:7080361-7080380 None:intergenic 20.0%
!!! CCAGTTTTTTTTATTGTTTA+TGG - chr4_2:7080358-7080377 Msa0585970:CDS 20.0%
!! GTTTATCAAGTTATAAATCT+TGG + chr4_2:7080401-7080420 None:intergenic 20.0%
!! TCAAACAAATCATTAAACTA+TGG + chr4_2:7080525-7080544 None:intergenic 20.0%
!!! AATTTATGTGTTGCTTATAT+TGG - chr4_2:7080577-7080596 Msa0585970:five_prime_UTR 20.0%
!!! TTTTCTAATCTTTATCCAAA+TGG - chr4_2:7081016-7081035 Msa0585970:five_prime_UTR 20.0%
!!! AGTTGATTTTGATTTTGATG+GGG + chr4_2:7079349-7079368 None:intergenic 25.0%
!!! CAAGTTGATTTTGATTTTGA+TGG + chr4_2:7079351-7079370 None:intergenic 25.0%
!!! TGTTTTAGAATTGGAACAAT+GGG + chr4_2:7079379-7079398 None:intergenic 25.0%
!!! TTGTTTTAGAATTGGAACAA+TGG + chr4_2:7079380-7079399 None:intergenic 25.0%
! TTTACAACAAAAACTCTTCT+GGG - chr4_2:7079501-7079520 Msa0585970:CDS 25.0%
!! TATTGGAGATTTAAGATCAA+TGG - chr4_2:7080230-7080249 Msa0585970:intron 25.0%
!! ATTGGAGATTTAAGATCAAT+GGG - chr4_2:7080231-7080250 Msa0585970:intron 25.0%
!!! GTTGTTTTATTTCAAAGCAA+TGG + chr4_2:7080297-7080316 None:intergenic 25.0%
!! TTTCACATTTTCTCTCAAAT+TGG + chr4_2:7080622-7080641 None:intergenic 25.0%
! ATCATTGTCTTCATTATATG+TGG + chr4_2:7080661-7080680 None:intergenic 25.0%
! TAATTGAAGTTGAGGATATT+TGG - chr4_2:7080933-7080952 Msa0585970:five_prime_UTR 25.0%
!! AAATTTTCTTACACAAACAG+TGG - chr4_2:7080985-7081004 Msa0585970:five_prime_UTR 25.0%
! CTTGTTTATAAGATTCCATT+TGG + chr4_2:7081034-7081053 None:intergenic 25.0%
! AATGGAATCTTATAAACAAG+AGG - chr4_2:7081034-7081053 Msa0585970:five_prime_UTR 25.0%
!!! AGTTGATTTTGATTTTGATG+GGG + chr4_2:7079349-7079368 None:intergenic 25.0%
!!! CAAGTTGATTTTGATTTTGA+TGG + chr4_2:7079351-7079370 None:intergenic 25.0%
!!! TGTTTTAGAATTGGAACAAT+GGG + chr4_2:7079379-7079398 None:intergenic 25.0%
!!! TTGTTTTAGAATTGGAACAA+TGG + chr4_2:7079380-7079399 None:intergenic 25.0%
! TTTACAACAAAAACTCTTCT+GGG - chr4_2:7079501-7079520 Msa0585970:CDS 25.0%
!! TATTGGAGATTTAAGATCAA+TGG - chr4_2:7080230-7080249 Msa0585970:intron 25.0%
!! ATTGGAGATTTAAGATCAAT+GGG - chr4_2:7080231-7080250 Msa0585970:intron 25.0%
!!! GTTGTTTTATTTCAAAGCAA+TGG + chr4_2:7080297-7080316 None:intergenic 25.0%
!! TTTCACATTTTCTCTCAAAT+TGG + chr4_2:7080622-7080641 None:intergenic 25.0%
! ATCATTGTCTTCATTATATG+TGG + chr4_2:7080661-7080680 None:intergenic 25.0%
! TAATTGAAGTTGAGGATATT+TGG - chr4_2:7080933-7080952 Msa0585970:five_prime_UTR 25.0%
!! AAATTTTCTTACACAAACAG+TGG - chr4_2:7080985-7081004 Msa0585970:five_prime_UTR 25.0%
! CTTGTTTATAAGATTCCATT+TGG + chr4_2:7081034-7081053 None:intergenic 25.0%
! AATGGAATCTTATAAACAAG+AGG - chr4_2:7081034-7081053 Msa0585970:five_prime_UTR 25.0%
GATTGAAGAGATTGAAGAAA+GGG + chr4_2:7079231-7079250 None:intergenic 30.0%
TGATTGAAGAGATTGAAGAA+AGG + chr4_2:7079232-7079251 None:intergenic 30.0%
!!! ACTTTGAGGTGGTTTTATTA+AGG + chr4_2:7079413-7079432 None:intergenic 30.0%
AATAAAACCACCTCAAAGTT+TGG - chr4_2:7079414-7079433 Msa0585970:CDS 30.0%
TTACAAAGAACCAAACTTTG+AGG + chr4_2:7079427-7079446 None:intergenic 30.0%
TTTGTAACCAAACCTATTCT+GGG - chr4_2:7079440-7079459 Msa0585970:CDS 30.0%
!! CAGAAGAGTTTTTGTTGTAA+AGG + chr4_2:7079502-7079521 None:intergenic 30.0%
CTTTACAACAAAAACTCTTC+TGG - chr4_2:7079500-7079519 Msa0585970:CDS 30.0%
ATCTAATTCTTCTTCTGTGA+TGG - chr4_2:7079798-7079817 Msa0585970:intron 30.0%
! AAGACTTTAAGAATTCAACC+TGG - chr4_2:7079829-7079848 Msa0585970:intron 30.0%
TTCTGAATCTGATGTTAGAA+AGG - chr4_2:7079855-7079874 Msa0585970:intron 30.0%
! GATTGATGATTTCGGTTAAT+AGG - chr4_2:7079935-7079954 Msa0585970:intron 30.0%
TGTGACACAATTATTGATCT+TGG + chr4_2:7080319-7080338 None:intergenic 30.0%
TAATATGAGAAGAGTAGTCA+CGG + chr4_2:7080758-7080777 None:intergenic 30.0%
GAGATATGACATACATTCAT+TGG + chr4_2:7080906-7080925 None:intergenic 30.0%
GATTGAAGAGATTGAAGAAA+GGG + chr4_2:7079231-7079250 None:intergenic 30.0%
TGATTGAAGAGATTGAAGAA+AGG + chr4_2:7079232-7079251 None:intergenic 30.0%
!!! ACTTTGAGGTGGTTTTATTA+AGG + chr4_2:7079413-7079432 None:intergenic 30.0%
AATAAAACCACCTCAAAGTT+TGG - chr4_2:7079414-7079433 Msa0585970:CDS 30.0%
TTACAAAGAACCAAACTTTG+AGG + chr4_2:7079427-7079446 None:intergenic 30.0%
TTTGTAACCAAACCTATTCT+GGG - chr4_2:7079440-7079459 Msa0585970:CDS 30.0%
!! CAGAAGAGTTTTTGTTGTAA+AGG + chr4_2:7079502-7079521 None:intergenic 30.0%
CTTTACAACAAAAACTCTTC+TGG - chr4_2:7079500-7079519 Msa0585970:CDS 30.0%
ATCTAATTCTTCTTCTGTGA+TGG - chr4_2:7079798-7079817 Msa0585970:intron 30.0%
! AAGACTTTAAGAATTCAACC+TGG - chr4_2:7079829-7079848 Msa0585970:intron 30.0%
TTCTGAATCTGATGTTAGAA+AGG - chr4_2:7079855-7079874 Msa0585970:intron 30.0%
! GATTGATGATTTCGGTTAAT+AGG - chr4_2:7079935-7079954 Msa0585970:intron 30.0%
TGTGACACAATTATTGATCT+TGG + chr4_2:7080319-7080338 None:intergenic 30.0%
TAATATGAGAAGAGTAGTCA+CGG + chr4_2:7080758-7080777 None:intergenic 30.0%
GAGATATGACATACATTCAT+TGG + chr4_2:7080906-7080925 None:intergenic 30.0%
GGTTGAAGAACAAAATAGAG+TGG + chr4_2:7079277-7079296 None:intergenic 35.0%
CTTTGTAACCAAACCTATTC+TGG - chr4_2:7079439-7079458 Msa0585970:CDS 35.0%
AATTCACAATGACCCAGAAT+AGG + chr4_2:7079455-7079474 None:intergenic 35.0%
!! GTAATGGATCTTGTGTTTCA+TGG - chr4_2:7079710-7079729 Msa0585970:intron 35.0%
!! TTTTAGACAACTTGCTTTGC+AGG - chr4_2:7079879-7079898 Msa0585970:intron 35.0%
CTTCTCATCTATGGTTTCAT+TGG + chr4_2:7080467-7080486 None:intergenic 35.0%
!! CTTTTGTTGCTTCTCATCTA+TGG + chr4_2:7080476-7080495 None:intergenic 35.0%
GACAATGATGAGTCATTTAG+AGG - chr4_2:7080672-7080691 Msa0585970:five_prime_UTR 35.0%
AGGAATGAATGATCCAGATT+GGG - chr4_2:7080692-7080711 Msa0585970:five_prime_UTR 35.0%
CCTCAACTTCAATTATGTTG+AGG + chr4_2:7080928-7080947 None:intergenic 35.0%
CCTCAACATAATTGAAGTTG+AGG - chr4_2:7080925-7080944 Msa0585970:five_prime_UTR 35.0%
GGTTGAAGAACAAAATAGAG+TGG + chr4_2:7079277-7079296 None:intergenic 35.0%
CTTTGTAACCAAACCTATTC+TGG - chr4_2:7079439-7079458 Msa0585970:CDS 35.0%
AATTCACAATGACCCAGAAT+AGG + chr4_2:7079455-7079474 None:intergenic 35.0%
!! GTAATGGATCTTGTGTTTCA+TGG - chr4_2:7079710-7079729 Msa0585970:intron 35.0%
!! TTTTAGACAACTTGCTTTGC+AGG - chr4_2:7079879-7079898 Msa0585970:intron 35.0%
CTTCTCATCTATGGTTTCAT+TGG + chr4_2:7080467-7080486 None:intergenic 35.0%
!! CTTTTGTTGCTTCTCATCTA+TGG + chr4_2:7080476-7080495 None:intergenic 35.0%
GACAATGATGAGTCATTTAG+AGG - chr4_2:7080672-7080691 Msa0585970:five_prime_UTR 35.0%
AGGAATGAATGATCCAGATT+GGG - chr4_2:7080692-7080711 Msa0585970:five_prime_UTR 35.0%
CCTCAACTTCAATTATGTTG+AGG + chr4_2:7080928-7080947 None:intergenic 35.0%
CCTCAACATAATTGAAGTTG+AGG - chr4_2:7080925-7080944 Msa0585970:five_prime_UTR 35.0%
GTCACGTTTGCATCAATCAA+TGG - chr4_2:7079130-7079149 Msa0585970:three_prime_UTR 40.0%
TAGAATTGGAACAATGGGCA+CGG + chr4_2:7079374-7079393 None:intergenic 40.0%
! CAAAGAACCAAACTTTGAGG+TGG + chr4_2:7079424-7079443 None:intergenic 40.0%
ACAATGACCCAGAATAGGTT+TGG + chr4_2:7079450-7079469 None:intergenic 40.0%
!! GTTGGTGTTATTGGTGGTAA+TGG - chr4_2:7079694-7079713 Msa0585970:intron 40.0%
TGTGTTTCATGGATGCAATC+TGG - chr4_2:7079721-7079740 Msa0585970:intron 40.0%
ATGGATGCAATCTGGAAGAA+AGG - chr4_2:7079729-7079748 Msa0585970:intron 40.0%
TGTGCAAAGGAAGAGATTGT+GGG - chr4_2:7080039-7080058 Msa0585970:intron 40.0%
GCAGTTTCACTTGATCAATG+AGG - chr4_2:7080064-7080083 Msa0585970:intron 40.0%
GAGGAATGAATGATCCAGAT+TGG - chr4_2:7080691-7080710 Msa0585970:five_prime_UTR 40.0%
GGAATGAATGATCCAGATTG+GGG - chr4_2:7080693-7080712 Msa0585970:five_prime_UTR 40.0%
AGCACCAACCAAATTTGGAT+GGG + chr4_2:7080868-7080887 None:intergenic 40.0%
!! CAAAGAGCACCAACCAAATT+TGG + chr4_2:7080873-7080892 None:intergenic 40.0%
GTCACGTTTGCATCAATCAA+TGG - chr4_2:7079130-7079149 Msa0585970:three_prime_UTR 40.0%
TAGAATTGGAACAATGGGCA+CGG + chr4_2:7079374-7079393 None:intergenic 40.0%
! CAAAGAACCAAACTTTGAGG+TGG + chr4_2:7079424-7079443 None:intergenic 40.0%
ACAATGACCCAGAATAGGTT+TGG + chr4_2:7079450-7079469 None:intergenic 40.0%
!! GTTGGTGTTATTGGTGGTAA+TGG - chr4_2:7079694-7079713 Msa0585970:intron 40.0%
TGTGTTTCATGGATGCAATC+TGG - chr4_2:7079721-7079740 Msa0585970:intron 40.0%
ATGGATGCAATCTGGAAGAA+AGG - chr4_2:7079729-7079748 Msa0585970:intron 40.0%
TGTGCAAAGGAAGAGATTGT+GGG - chr4_2:7080039-7080058 Msa0585970:intron 40.0%
GCAGTTTCACTTGATCAATG+AGG - chr4_2:7080064-7080083 Msa0585970:intron 40.0%
GAGGAATGAATGATCCAGAT+TGG - chr4_2:7080691-7080710 Msa0585970:five_prime_UTR 40.0%
GGAATGAATGATCCAGATTG+GGG - chr4_2:7080693-7080712 Msa0585970:five_prime_UTR 40.0%
AGCACCAACCAAATTTGGAT+GGG + chr4_2:7080868-7080887 None:intergenic 40.0%
!! CAAAGAGCACCAACCAAATT+TGG + chr4_2:7080873-7080892 None:intergenic 40.0%
TGATTGATGCAAACGTGACG+TGG + chr4_2:7079129-7079148 None:intergenic 45.0%
!! GCTACTGCTGTTGGTGTTAT+TGG - chr4_2:7079685-7079704 Msa0585970:intron 45.0%
!! ACTGCTGTTGGTGTTATTGG+TGG - chr4_2:7079688-7079707 Msa0585970:intron 45.0%
!! ACATCAGATTCAGAAGCACC+AGG + chr4_2:7079850-7079869 None:intergenic 45.0%
TACCATCCAGATGTGTGCAA+AGG - chr4_2:7080026-7080045 Msa0585970:intron 45.0%
TTCCTTTGCACACATCTGGA+TGG + chr4_2:7080031-7080050 None:intergenic 45.0%
TCTCTTCCTTTGCACACATC+TGG + chr4_2:7080035-7080054 None:intergenic 45.0%
GTGTGCAAAGGAAGAGATTG+TGG - chr4_2:7080038-7080057 Msa0585970:intron 45.0%
GTGCAAAGGAAGAGATTGTG+GGG - chr4_2:7080040-7080059 Msa0585970:intron 45.0%
ATGATCCAGATTGGGGCTAT+TGG - chr4_2:7080700-7080719 Msa0585970:five_prime_UTR 45.0%
TGATCCAGATTGGGGCTATT+GGG - chr4_2:7080701-7080720 Msa0585970:five_prime_UTR 45.0%
ATTGGGGCTATTGGGAAGAA+TGG - chr4_2:7080709-7080728 Msa0585970:five_prime_UTR 45.0%
GGCTATTGGGAAGAATGGAT+GGG - chr4_2:7080714-7080733 Msa0585970:five_prime_UTR 45.0%
ATTGGGAAGAATGGATGGGT+TGG - chr4_2:7080718-7080737 Msa0585970:five_prime_UTR 45.0%
! TTGGGAAGAATGGATGGGTT+GGG - chr4_2:7080719-7080738 Msa0585970:five_prime_UTR 45.0%
ACTTGTCCCCCATCCAAATT+TGG - chr4_2:7080857-7080876 Msa0585970:five_prime_UTR 45.0%
CACCAACCAAATTTGGATGG+GGG + chr4_2:7080866-7080885 None:intergenic 45.0%
GCACCAACCAAATTTGGATG+GGG + chr4_2:7080867-7080886 None:intergenic 45.0%
GAGCACCAACCAAATTTGGA+TGG + chr4_2:7080869-7080888 None:intergenic 45.0%
TGATTGATGCAAACGTGACG+TGG + chr4_2:7079129-7079148 None:intergenic 45.0%
!! GCTACTGCTGTTGGTGTTAT+TGG - chr4_2:7079685-7079704 Msa0585970:intron 45.0%
!! ACTGCTGTTGGTGTTATTGG+TGG - chr4_2:7079688-7079707 Msa0585970:intron 45.0%
!! ACATCAGATTCAGAAGCACC+AGG + chr4_2:7079850-7079869 None:intergenic 45.0%
TACCATCCAGATGTGTGCAA+AGG - chr4_2:7080026-7080045 Msa0585970:intron 45.0%
TTCCTTTGCACACATCTGGA+TGG + chr4_2:7080031-7080050 None:intergenic 45.0%
TCTCTTCCTTTGCACACATC+TGG + chr4_2:7080035-7080054 None:intergenic 45.0%
GTGTGCAAAGGAAGAGATTG+TGG - chr4_2:7080038-7080057 Msa0585970:intron 45.0%
GTGCAAAGGAAGAGATTGTG+GGG - chr4_2:7080040-7080059 Msa0585970:intron 45.0%
ATGATCCAGATTGGGGCTAT+TGG - chr4_2:7080700-7080719 Msa0585970:five_prime_UTR 45.0%
TGATCCAGATTGGGGCTATT+GGG - chr4_2:7080701-7080720 Msa0585970:five_prime_UTR 45.0%
ATTGGGGCTATTGGGAAGAA+TGG - chr4_2:7080709-7080728 Msa0585970:five_prime_UTR 45.0%
GGCTATTGGGAAGAATGGAT+GGG - chr4_2:7080714-7080733 Msa0585970:five_prime_UTR 45.0%
ATTGGGAAGAATGGATGGGT+TGG - chr4_2:7080718-7080737 Msa0585970:five_prime_UTR 45.0%
! TTGGGAAGAATGGATGGGTT+GGG - chr4_2:7080719-7080738 Msa0585970:five_prime_UTR 45.0%
ACTTGTCCCCCATCCAAATT+TGG - chr4_2:7080857-7080876 Msa0585970:five_prime_UTR 45.0%
CACCAACCAAATTTGGATGG+GGG + chr4_2:7080866-7080885 None:intergenic 45.0%
GCACCAACCAAATTTGGATG+GGG + chr4_2:7080867-7080886 None:intergenic 45.0%
GAGCACCAACCAAATTTGGA+TGG + chr4_2:7080869-7080888 None:intergenic 45.0%
!!! TTTTGATTTTTTTTAAAAAT+AGG + chr4_2:7079298-7079317 None:intergenic 5.0%
!!! TTTTGATTTTTTTTAAAAAT+AGG + chr4_2:7079298-7079317 None:intergenic 5.0%
TCTTCCCAATAGCCCCAATC+TGG + chr4_2:7080708-7080727 None:intergenic 50.0%
GGGCTATTGGGAAGAATGGA+TGG - chr4_2:7080713-7080732 Msa0585970:five_prime_UTR 50.0%
!! GAAGAATGGATGGGTTGGGA+AGG - chr4_2:7080723-7080742 Msa0585970:five_prime_UTR 50.0%
TTGGATGGGGGACAAGTAGT+AGG + chr4_2:7080854-7080873 None:intergenic 50.0%
GTCCCCCATCCAAATTTGGT+TGG - chr4_2:7080861-7080880 Msa0585970:five_prime_UTR 50.0%
GCAAACGTGACGTGGAATCT+CGG + chr4_2:7079121-7079140 None:intergenic 50.0%
TCTTCCCAATAGCCCCAATC+TGG + chr4_2:7080708-7080727 None:intergenic 50.0%
GGGCTATTGGGAAGAATGGA+TGG - chr4_2:7080713-7080732 Msa0585970:five_prime_UTR 50.0%
!! GAAGAATGGATGGGTTGGGA+AGG - chr4_2:7080723-7080742 Msa0585970:five_prime_UTR 50.0%
TTGGATGGGGGACAAGTAGT+AGG + chr4_2:7080854-7080873 None:intergenic 50.0%
GTCCCCCATCCAAATTTGGT+TGG - chr4_2:7080861-7080880 Msa0585970:five_prime_UTR 50.0%
ATGGCTGCTGCTACTGCTGT+TGG - chr4_2:7079676-7079695 Msa0585970:intron 55.0%
ATGGCTGCTGCTACTGCTGT+TGG - chr4_2:7079676-7079695 Msa0585970:intron 55.0%
!! TGGATGGGTTGGGAAGGAGC+AGG - chr4_2:7080729-7080748 Msa0585970:five_prime_UTR 60.0%
!! TGGATGGGTTGGGAAGGAGC+AGG - chr4_2:7080729-7080748 Msa0585970:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 7079119 7081057 7079119 ID=Msa0585970;Name=Msa0585970
chr4_2 mRNA 7079119 7081057 7079119 ID=Msa0585970-mRNA-1;Parent=Msa0585970;Name=Msa0585970-mRNA-1;_AED=0.14;_eAED=0.14;_QI=557|1|1|1|1|1|3|266|158
chr4_2 exon 7079119 7079567 7079119 ID=Msa0585970-mRNA-1:exon:1785;Parent=Msa0585970-mRNA-1
chr4_2 exon 7080082 7080150 7080082 ID=Msa0585970-mRNA-1:exon:1784;Parent=Msa0585970-mRNA-1
chr4_2 exon 7080276 7081057 7080276 ID=Msa0585970-mRNA-1:exon:1783;Parent=Msa0585970-mRNA-1
chr4_2 five_prime_UTR 7080501 7081057 7080501 ID=Msa0585970-mRNA-1:five_prime_utr;Parent=Msa0585970-mRNA-1
chr4_2 CDS 7080276 7080500 7080276 ID=Msa0585970-mRNA-1:cds;Parent=Msa0585970-mRNA-1
chr4_2 CDS 7080082 7080150 7080082 ID=Msa0585970-mRNA-1:cds;Parent=Msa0585970-mRNA-1
chr4_2 CDS 7079385 7079567 7079385 ID=Msa0585970-mRNA-1:cds;Parent=Msa0585970-mRNA-1
chr4_2 three_prime_UTR 7079119 7079384 7079119 ID=Msa0585970-mRNA-1:three_prime_utr;Parent=Msa0585970-mRNA-1
Gene Sequence

>Msa0585970

ATGGCTGCTGCTACTGCTGTTGGTGTTATTGGTGGTAATGGATCTTGTGTTTCATGGATGCAATCTGGAAGAAAGGAAAAGAAACAAAATAAAATGAATAAAGTTAGGGTTTGTTGCTCTAAATCTAATTCTTCTTCTGTGATGGATCCTTATAAGACTTTAAGAATTCAACCTGGTGCTTCTGAATCTGATGTTAGAAAGGCTTTTAGACAACTTGCTTTGCAGTACCATCCAGATGTGTGCAAAGGAAGAGATTGTGGGGTGCAGTTTCACTTGATCAATGAGGCTTATGATATTGTGATGTCCAATTTGAGAGAAAATGTGAAAGAAACTTATGAGACGACCACATATAATGAAGACAATGATGAGTCATTTAGAGGAATGAATGATCCAGATTGGGGCTATTGGGAAGAATGGATGGGTTGGGAAGGAGCAGGAATCCGTGACTACTCTTCTCATATTAATCCTTACATTTAA
Protein sequence

>Msa0585970

MAAATAVGVIGGNGSCVSWMQSGRKEKKQNKMNKVRVCCSKSNSSSVMDPYKTLRIQPGASESDVRKAFRQLALQYHPDVCKGRDCGVQFHLINEAYDIVMSNLRENVKETYETTTYNEDNDESFRGMNDPDWGYWEEWMGWEGAGIRDYSSHINPYI*