AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0626020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0626020 MtrunA17_Chr4g0001611 92.547 161 7 2 1 156 1 161 6.61e-107 301
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0626020 AT1G80920.1 62.727 110 40 1 46 155 54 162 7.06e-48 152
Msa0626020 AT5G59610.1 29.167 144 86 3 11 144 43 180 2.60e-13 65.9
Msa0626020 AT5G59610.4 29.167 144 86 3 11 144 43 180 2.60e-13 65.9

Find 63 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTAACAAAACCTATTC+TGG 0.123804 4_3:-5406898 Msa0626020:five_prime_UTR
ACTACTGCTGTTGGTGTTAT+TGG 0.156544 4_3:-5406654 Msa0626020:CDS
CAAAGAGCACCAACCAAATT+TGG 0.237220 4_3:+5405456 None:intergenic
ATGTTAAATATTTCAAAGTT+TGG 0.284170 4_3:-5406800 Msa0626020:five_prime_UTR
ATGATCCAGATTGGGGCTAT+TGG 0.284504 4_3:-5405626 Msa0626020:CDS
TGAATTCTCAAAGTCTTATA+AGG 0.285846 4_3:+5406522 None:intergenic
AATAAAACCACCTCAAAGTT+TGG 0.298635 4_3:-5406923 Msa0626020:five_prime_UTR
AAAATAAGATGAATAAAGTT+AGG 0.311546 4_3:-5406584 Msa0626020:CDS
TATGTTCTTATCTTAAAGTT+TGG 0.324007 4_3:-5406862 Msa0626020:five_prime_UTR
AATCATTATTTAATAGCGTT+TGG 0.326506 4_3:-5407028 Msa0626020:five_prime_UTR
CTTGTTTATAAGATTCCATT+TGG 0.340253 4_3:+5405310 None:intergenic
GTAATGGATCTTGTGTTTCA+TGG 0.354259 4_3:-5406629 Msa0626020:CDS
TCTCTTCCTTTGCACACATC+TGG 0.358215 4_3:+5406281 None:intergenic
GAGAATGATGAGTCATTTAG+AGG 0.362832 4_3:-5405654 Msa0626020:CDS
TTAGTTATTATGTGTGAAAT+CGG 0.370612 4_3:+5407183 None:intergenic
TGATCCAGATTGGGGCTATT+GGG 0.375755 4_3:-5405625 Msa0626020:CDS
GTTGGTGTTATTGGTGGTAA+TGG 0.380315 4_3:-5406645 Msa0626020:CDS
GAGATATGACATACATTCAT+TGG 0.391558 4_3:+5405423 None:intergenic
TGTTTCATGGATTGGAAGAA+AGG 0.400791 4_3:-5406616 Msa0626020:CDS
TTTGTAACAAAACCTATTCT+GGG 0.402862 4_3:-5406897 Msa0626020:five_prime_UTR
TCTTCCCAATAGCCCCAATC+TGG 0.418268 4_3:+5405621 None:intergenic
GGGCTATTGGGAAGAATGGA+TGG 0.423463 4_3:-5405613 Msa0626020:CDS
GAGGAATGAATGATCCAGAT+TGG 0.426354 4_3:-5405635 Msa0626020:CDS
AGGAATGAATGATCCAGATT+GGG 0.438090 4_3:-5405634 Msa0626020:CDS
AATTCACAATGACCCAGAAT+AGG 0.446531 4_3:+5406885 None:intergenic
GATTGAAGAGATTGAAGAAA+GGG 0.453780 4_3:+5407116 None:intergenic
ATCATTCTCTTCATTATATG+TGG 0.460927 4_3:+5405668 None:intergenic
GGATCTTGTGTTTCATGGAT+TGG 0.460943 4_3:-5406624 Msa0626020:CDS
ATTGGGGCTATTGGGAAGAA+TGG 0.464008 4_3:-5405617 Msa0626020:CDS
TTCTGAATCTGATGTTAGAA+AGG 0.473384 4_3:-5406490 Msa0626020:CDS
AAATAAGATGAATAAAGTTA+GGG 0.476392 4_3:-5406583 Msa0626020:CDS
ATCTAAATCTTCTTCTGTGA+TGG 0.478827 4_3:-5406547 Msa0626020:CDS
TTGGGAAGAATGGATGGGTT+GGG 0.486704 4_3:-5405607 Msa0626020:CDS
ATTGGGAAGAATGGATGGGT+TGG 0.503999 4_3:-5405608 Msa0626020:CDS
TTACAAAGAACCAAACTTTG+AGG 0.507727 4_3:+5406913 None:intergenic
TTCCTTTGCACACATCTGGA+TGG 0.515608 4_3:+5406285 None:intergenic
GTCACGTTTGCATCAATCAA+TGG 0.519392 4_3:-5407213 Msa0626020:five_prime_UTR
ACTGCTGTTGGTGTTATTGG+TGG 0.520448 4_3:-5406651 Msa0626020:CDS
GGCTATTGGGAAGAATGGAT+GGG 0.528910 4_3:-5405612 Msa0626020:CDS
GTGTGCAAAGGAAGAGATTG+TGG 0.529500 4_3:-5406275 Msa0626020:CDS
GAGCACCAACCAAATTTGGA+TGG 0.537248 4_3:+5405460 None:intergenic
CCTCAACATAATTGAAGTTG+AGG 0.538944 4_3:-5405401 Msa0626020:three_prime_UTR
TGATTGAAGAGATTGAAGAA+AGG 0.546658 4_3:+5407115 None:intergenic
AGCACCAACCAAATTTGGAT+GGG 0.548146 4_3:+5405461 None:intergenic
GAAGAATGGATGGGTTGGGA+AGG 0.548816 4_3:-5405603 Msa0626020:CDS
TGGATGGGTTGGGAAGGAGC+AGG 0.559751 4_3:-5405597 Msa0626020:CDS
CCTCAACTTCAATTATGTTG+AGG 0.569740 4_3:+5405401 None:intergenic
TGTGCAAAGGAAGAGATTGT+GGG 0.575497 4_3:-5406274 Msa0626020:CDS
GCAGTTTCACTTAATCAATG+AGG 0.585631 4_3:-5406249 Msa0626020:CDS
AATGGAATCTTATAAACAAG+AGG 0.587710 4_3:-5405307 Msa0626020:three_prime_UTR
ATGGCTGCTACTACTGCTGT+TGG 0.598684 4_3:-5406663 Msa0626020:CDS
GCACCAACCAAATTTGGATG+GGG 0.605222 4_3:+5405462 None:intergenic
CAAAGAACCAAACTTTGAGG+TGG 0.605813 4_3:+5406916 None:intergenic
GGTTGAAGAACAAAATAGAG+TGG 0.607277 4_3:+5407070 None:intergenic
TACCATCCAGATGTGTGCAA+AGG 0.620033 4_3:-5406287 Msa0626020:CDS
TAATATGAGAAGAGTAGTCA+CGG 0.620194 4_3:+5405571 None:intergenic
TAGAATTGGAACAATGGGCA+CGG 0.629877 4_3:+5406967 None:intergenic
GGAATGAATGATCCAGATTG+GGG 0.639140 4_3:-5405633 Msa0626020:CDS
TGATTGATGCAAACGTGACG+TGG 0.641491 4_3:+5407217 None:intergenic
ACATCAGATTCAGAAGCACC+AGG 0.654245 4_3:+5406498 None:intergenic
CACCAACCAAATTTGGATGG+GGG 0.668622 4_3:+5405463 None:intergenic
AAGACTTTGAGAATTCAACC+TGG 0.677099 4_3:-5406516 Msa0626020:CDS
GTGCAAAGGAAGAGATTGTG+GGG 0.740567 4_3:-5406273 Msa0626020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTATTAATATTGAATATT+TGG - chr4_3:5406201-5406220 Msa0626020:intron 10.0%
!! TGTATTAATATTGAATATTT+GGG - chr4_3:5406202-5406221 Msa0626020:intron 10.0%
!! AATTTATTGTTATAATTGTT+TGG - chr4_3:5406365-5406384 Msa0626020:intron 10.0%
!! ATTTATTGTTATAATTGTTT+GGG - chr4_3:5406366-5406385 Msa0626020:intron 10.0%
!!! TTTTAGATCATAAAAATATA+TGG - chr4_3:5406400-5406419 Msa0626020:intron 10.0%
!! ATGTATTAATATTGAATATT+TGG - chr4_3:5406201-5406220 Msa0626020:intron 10.0%
!! TGTATTAATATTGAATATTT+GGG - chr4_3:5406202-5406221 Msa0626020:intron 10.0%
!! AATTTATTGTTATAATTGTT+TGG - chr4_3:5406365-5406384 Msa0626020:intron 10.0%
!! ATTTATTGTTATAATTGTTT+GGG - chr4_3:5406366-5406385 Msa0626020:intron 10.0%
!!! TTTTAGATCATAAAAATATA+TGG - chr4_3:5406400-5406419 Msa0626020:intron 10.0%
!!! ATTTTGATTTTGATTTTGAT+GGG + chr4_3:5405536-5405555 None:intergenic 15.0%
!! ATGTTAAATATTTCAAAGTT+TGG - chr4_3:5405730-5405749 Msa0626020:intron 15.0%
!!! TTTATAACAAAGAGTTTTTT+GGG - chr4_3:5405819-5405838 Msa0626020:intron 15.0%
!! AAAATAAGATGAATAAAGTT+AGG - chr4_3:5405946-5405965 Msa0626020:intron 15.0%
!! AAATAAGATGAATAAAGTTA+GGG - chr4_3:5405947-5405966 Msa0626020:intron 15.0%
!!! TTTTTTATTGTTTATGGTTT+TGG - chr4_3:5406568-5406587 Msa0626020:CDS 15.0%
!!! AATTTGTTTTTATCTTCTTA+TGG - chr4_3:5406701-5406720 Msa0626020:five_prime_UTR 15.0%
!!! ATTTGTTTTTATCTTCTTAT+GGG - chr4_3:5406702-5406721 Msa0626020:five_prime_UTR 15.0%
!! CATAAATTTCAATTAGATAA+TGG + chr4_3:5406769-5406788 None:intergenic 15.0%
!!! CATAAACTTTTTTAAATGTA+AGG + chr4_3:5406987-5407006 None:intergenic 15.0%
!!! ATTTTGATTTTGATTTTGAT+GGG + chr4_3:5405536-5405555 None:intergenic 15.0%
!! ATGTTAAATATTTCAAAGTT+TGG - chr4_3:5405730-5405749 Msa0626020:intron 15.0%
!!! TTTATAACAAAGAGTTTTTT+GGG - chr4_3:5405819-5405838 Msa0626020:intron 15.0%
!! AAAATAAGATGAATAAAGTT+AGG - chr4_3:5405946-5405965 Msa0626020:intron 15.0%
!! AAATAAGATGAATAAAGTTA+GGG - chr4_3:5405947-5405966 Msa0626020:intron 15.0%
!!! TTTTTTATTGTTTATGGTTT+TGG - chr4_3:5406568-5406587 Msa0626020:CDS 15.0%
!!! AATTTGTTTTTATCTTCTTA+TGG - chr4_3:5406701-5406720 Msa0626020:five_prime_UTR 15.0%
!!! ATTTGTTTTTATCTTCTTAT+GGG - chr4_3:5406702-5406721 Msa0626020:five_prime_UTR 15.0%
!! CATAAATTTCAATTAGATAA+TGG + chr4_3:5406769-5406788 None:intergenic 15.0%
!!! CATAAACTTTTTTAAATGTA+AGG + chr4_3:5406987-5407006 None:intergenic 15.0%
!! TTAGTTATTATGTGTGAAAT+CGG + chr4_3:5405350-5405369 None:intergenic 20.0%
!! AATCATTATTTAATAGCGTT+TGG - chr4_3:5405502-5405521 Msa0626020:three_prime_UTR 20.0%
!!! TTTTGATTTTGATTTTGATG+GGG + chr4_3:5405535-5405554 None:intergenic 20.0%
!!! GATTTTGATTTTGATTTTGA+TGG + chr4_3:5405537-5405556 None:intergenic 20.0%
!!! GAAGATATTGTTTTTAGAAT+TGG + chr4_3:5405580-5405599 None:intergenic 20.0%
!! TATGTTCTTATCTTAAAGTT+TGG - chr4_3:5405668-5405687 Msa0626020:CDS 20.0%
!! GAGTTAGATATAAAAAAGTT+TGG - chr4_3:5405793-5405812 Msa0626020:intron 20.0%
!!! CTTTATAACAAAGAGTTTTT+TGG - chr4_3:5405818-5405837 Msa0626020:intron 20.0%
!!! AGAATTTGTGATTTTAAGAA+TGG - chr4_3:5405848-5405867 Msa0626020:intron 20.0%
!!! ACTAATTTGATTGATGATTT+TGG - chr4_3:5406112-5406131 Msa0626020:intron 20.0%
!! AACTATGCAATGAAAATTAA+AGG + chr4_3:5406345-5406364 None:intergenic 20.0%
!!! GTTGTTTTATTTCAAATCAA+TGG + chr4_3:5406501-5406520 None:intergenic 20.0%
!! ACAATAAAAAAAACTGGATT+TGG + chr4_3:5406559-5406578 None:intergenic 20.0%
!! CCATAAACAATAAAAAAAAC+TGG + chr4_3:5406565-5406584 None:intergenic 20.0%
!!! CCAGTTTTTTTTATTGTTTA+TGG - chr4_3:5406562-5406581 Msa0626020:CDS 20.0%
!! GTTTATCAAGTTATAAATCT+TGG + chr4_3:5406605-5406624 None:intergenic 20.0%
!! TCAAACAAATCATTAAACTA+TGG + chr4_3:5406729-5406748 None:intergenic 20.0%
!!! AATTTATGTGTTGCTTATAT+TGG - chr4_3:5406781-5406800 Msa0626020:five_prime_UTR 20.0%
!!! TTTTCTAATCTTTATCCAAA+TGG - chr4_3:5407205-5407224 Msa0626020:five_prime_UTR 20.0%
!! TTAGTTATTATGTGTGAAAT+CGG + chr4_3:5405350-5405369 None:intergenic 20.0%
!! AATCATTATTTAATAGCGTT+TGG - chr4_3:5405502-5405521 Msa0626020:three_prime_UTR 20.0%
!!! TTTTGATTTTGATTTTGATG+GGG + chr4_3:5405535-5405554 None:intergenic 20.0%
!!! GATTTTGATTTTGATTTTGA+TGG + chr4_3:5405537-5405556 None:intergenic 20.0%
!!! GAAGATATTGTTTTTAGAAT+TGG + chr4_3:5405580-5405599 None:intergenic 20.0%
!! TATGTTCTTATCTTAAAGTT+TGG - chr4_3:5405668-5405687 Msa0626020:CDS 20.0%
!! GAGTTAGATATAAAAAAGTT+TGG - chr4_3:5405793-5405812 Msa0626020:intron 20.0%
!!! CTTTATAACAAAGAGTTTTT+TGG - chr4_3:5405818-5405837 Msa0626020:intron 20.0%
!!! AGAATTTGTGATTTTAAGAA+TGG - chr4_3:5405848-5405867 Msa0626020:intron 20.0%
!!! ACTAATTTGATTGATGATTT+TGG - chr4_3:5406112-5406131 Msa0626020:intron 20.0%
!! AACTATGCAATGAAAATTAA+AGG + chr4_3:5406345-5406364 None:intergenic 20.0%
!!! GTTGTTTTATTTCAAATCAA+TGG + chr4_3:5406501-5406520 None:intergenic 20.0%
!! ACAATAAAAAAAACTGGATT+TGG + chr4_3:5406559-5406578 None:intergenic 20.0%
!! CCATAAACAATAAAAAAAAC+TGG + chr4_3:5406565-5406584 None:intergenic 20.0%
!!! CCAGTTTTTTTTATTGTTTA+TGG - chr4_3:5406562-5406581 Msa0626020:CDS 20.0%
!! GTTTATCAAGTTATAAATCT+TGG + chr4_3:5406605-5406624 None:intergenic 20.0%
!! TCAAACAAATCATTAAACTA+TGG + chr4_3:5406729-5406748 None:intergenic 20.0%
!!! AATTTATGTGTTGCTTATAT+TGG - chr4_3:5406781-5406800 Msa0626020:five_prime_UTR 20.0%
!!! TTTTCTAATCTTTATCCAAA+TGG - chr4_3:5407205-5407224 Msa0626020:five_prime_UTR 20.0%
!!! GTTTTTAGAATTGGAACAAT+GGG + chr4_3:5405571-5405590 None:intergenic 25.0%
!!! TGTTTTTAGAATTGGAACAA+TGG + chr4_3:5405572-5405591 None:intergenic 25.0%
! TTTGTAACAAAACCTATTCT+GGG - chr4_3:5405633-5405652 Msa0626020:CDS 25.0%
! TTTACAACAAAAACTCTTCT+GGG - chr4_3:5405694-5405713 Msa0626020:CDS 25.0%
!! TGAATTCTCAAAGTCTTATA+AGG + chr4_3:5406011-5406030 None:intergenic 25.0%
!! TGGATTTGTAATCTTGATTT+TGG - chr4_3:5406132-5406151 Msa0626020:intron 25.0%
! CATTATACATGAAGAAAGAA+AGG + chr4_3:5406184-5406203 None:intergenic 25.0%
!! ATATGGATATTGATTGACAT+TGG - chr4_3:5406417-5406436 Msa0626020:intron 25.0%
! ATTGGAGATATAAGATCAAT+GGG - chr4_3:5406435-5406454 Msa0626020:intron 25.0%
!! TTTCACATTTTCTCTCAAAT+TGG + chr4_3:5406826-5406845 None:intergenic 25.0%
! ATCATTCTCTTCATTATATG+TGG + chr4_3:5406865-5406884 None:intergenic 25.0%
! CTTGTTTATAAGATTCCATT+TGG + chr4_3:5407223-5407242 None:intergenic 25.0%
! AATGGAATCTTATAAACAAG+AGG - chr4_3:5407223-5407242 Msa0626020:five_prime_UTR 25.0%
!!! GTTTTTAGAATTGGAACAAT+GGG + chr4_3:5405571-5405590 None:intergenic 25.0%
!!! TGTTTTTAGAATTGGAACAA+TGG + chr4_3:5405572-5405591 None:intergenic 25.0%
! TTTGTAACAAAACCTATTCT+GGG - chr4_3:5405633-5405652 Msa0626020:CDS 25.0%
! TTTACAACAAAAACTCTTCT+GGG - chr4_3:5405694-5405713 Msa0626020:CDS 25.0%
!! TGAATTCTCAAAGTCTTATA+AGG + chr4_3:5406011-5406030 None:intergenic 25.0%
!! TGGATTTGTAATCTTGATTT+TGG - chr4_3:5406132-5406151 Msa0626020:intron 25.0%
! CATTATACATGAAGAAAGAA+AGG + chr4_3:5406184-5406203 None:intergenic 25.0%
!! ATATGGATATTGATTGACAT+TGG - chr4_3:5406417-5406436 Msa0626020:intron 25.0%
! ATTGGAGATATAAGATCAAT+GGG - chr4_3:5406435-5406454 Msa0626020:intron 25.0%
!! TTTCACATTTTCTCTCAAAT+TGG + chr4_3:5406826-5406845 None:intergenic 25.0%
! ATCATTCTCTTCATTATATG+TGG + chr4_3:5406865-5406884 None:intergenic 25.0%
! CTTGTTTATAAGATTCCATT+TGG + chr4_3:5407223-5407242 None:intergenic 25.0%
! AATGGAATCTTATAAACAAG+AGG - chr4_3:5407223-5407242 Msa0626020:five_prime_UTR 25.0%
GATTGAAGAGATTGAAGAAA+GGG + chr4_3:5405417-5405436 None:intergenic 30.0%
TGATTGAAGAGATTGAAGAA+AGG + chr4_3:5405418-5405437 None:intergenic 30.0%
!!! ACTTTGAGGTGGTTTTATTA+AGG + chr4_3:5405606-5405625 None:intergenic 30.0%
AATAAAACCACCTCAAAGTT+TGG - chr4_3:5405607-5405626 Msa0626020:CDS 30.0%
TTACAAAGAACCAAACTTTG+AGG + chr4_3:5405620-5405639 None:intergenic 30.0%
CTTTGTAACAAAACCTATTC+TGG - chr4_3:5405632-5405651 Msa0626020:CDS 30.0%
!! CAGAAGAGTTTTTGTTGTAA+AGG + chr4_3:5405695-5405714 None:intergenic 30.0%
CTTTACAACAAAAACTCTTC+TGG - chr4_3:5405693-5405712 Msa0626020:CDS 30.0%
ATCTAAATCTTCTTCTGTGA+TGG - chr4_3:5405983-5406002 Msa0626020:intron 30.0%
TTCTGAATCTGATGTTAGAA+AGG - chr4_3:5406040-5406059 Msa0626020:intron 30.0%
! GGATAGCTTAGAGAAATTTT+AGG + chr4_3:5406324-5406343 None:intergenic 30.0%
CATTGGAGATATAAGATCAA+TGG - chr4_3:5406434-5406453 Msa0626020:intron 30.0%
TGTGACACAATTATTGATCT+TGG + chr4_3:5406523-5406542 None:intergenic 30.0%
TAATATGAGAAGAGTAGTCA+CGG + chr4_3:5406962-5406981 None:intergenic 30.0%
GAGATATGACATACATTCAT+TGG + chr4_3:5407110-5407129 None:intergenic 30.0%
GATTGAAGAGATTGAAGAAA+GGG + chr4_3:5405417-5405436 None:intergenic 30.0%
TGATTGAAGAGATTGAAGAA+AGG + chr4_3:5405418-5405437 None:intergenic 30.0%
!!! ACTTTGAGGTGGTTTTATTA+AGG + chr4_3:5405606-5405625 None:intergenic 30.0%
AATAAAACCACCTCAAAGTT+TGG - chr4_3:5405607-5405626 Msa0626020:CDS 30.0%
TTACAAAGAACCAAACTTTG+AGG + chr4_3:5405620-5405639 None:intergenic 30.0%
CTTTGTAACAAAACCTATTC+TGG - chr4_3:5405632-5405651 Msa0626020:CDS 30.0%
!! CAGAAGAGTTTTTGTTGTAA+AGG + chr4_3:5405695-5405714 None:intergenic 30.0%
CTTTACAACAAAAACTCTTC+TGG - chr4_3:5405693-5405712 Msa0626020:CDS 30.0%
ATCTAAATCTTCTTCTGTGA+TGG - chr4_3:5405983-5406002 Msa0626020:intron 30.0%
TTCTGAATCTGATGTTAGAA+AGG - chr4_3:5406040-5406059 Msa0626020:intron 30.0%
! GGATAGCTTAGAGAAATTTT+AGG + chr4_3:5406324-5406343 None:intergenic 30.0%
CATTGGAGATATAAGATCAA+TGG - chr4_3:5406434-5406453 Msa0626020:intron 30.0%
TGTGACACAATTATTGATCT+TGG + chr4_3:5406523-5406542 None:intergenic 30.0%
TAATATGAGAAGAGTAGTCA+CGG + chr4_3:5406962-5406981 None:intergenic 30.0%
GAGATATGACATACATTCAT+TGG + chr4_3:5407110-5407129 None:intergenic 30.0%
GGTTGAAGAACAAAATAGAG+TGG + chr4_3:5405463-5405482 None:intergenic 35.0%
AATTCACAATGACCCAGAAT+AGG + chr4_3:5405648-5405667 None:intergenic 35.0%
!! GTAATGGATCTTGTGTTTCA+TGG - chr4_3:5405901-5405920 Msa0626020:intron 35.0%
!! TGTTTCATGGATTGGAAGAA+AGG - chr4_3:5405914-5405933 Msa0626020:intron 35.0%
! AAGACTTTGAGAATTCAACC+TGG - chr4_3:5406014-5406033 Msa0626020:intron 35.0%
!! TTTTAGACAACTTGCTTTGC+AGG - chr4_3:5406064-5406083 Msa0626020:intron 35.0%
GCAGTTTCACTTAATCAATG+AGG - chr4_3:5406281-5406300 Msa0626020:CDS 35.0%
CTTCTCATCTATGGTTTCAT+TGG + chr4_3:5406671-5406690 None:intergenic 35.0%
!! CTTTTGTTGCTTCTCATCTA+TGG + chr4_3:5406680-5406699 None:intergenic 35.0%
GAGAATGATGAGTCATTTAG+AGG - chr4_3:5406876-5406895 Msa0626020:five_prime_UTR 35.0%
AGGAATGAATGATCCAGATT+GGG - chr4_3:5406896-5406915 Msa0626020:five_prime_UTR 35.0%
CCTCAACTTCAATTATGTTG+AGG + chr4_3:5407132-5407151 None:intergenic 35.0%
CCTCAACATAATTGAAGTTG+AGG - chr4_3:5407129-5407148 Msa0626020:five_prime_UTR 35.0%
GGTTGAAGAACAAAATAGAG+TGG + chr4_3:5405463-5405482 None:intergenic 35.0%
AATTCACAATGACCCAGAAT+AGG + chr4_3:5405648-5405667 None:intergenic 35.0%
!! GTAATGGATCTTGTGTTTCA+TGG - chr4_3:5405901-5405920 Msa0626020:intron 35.0%
!! TGTTTCATGGATTGGAAGAA+AGG - chr4_3:5405914-5405933 Msa0626020:intron 35.0%
! AAGACTTTGAGAATTCAACC+TGG - chr4_3:5406014-5406033 Msa0626020:intron 35.0%
!! TTTTAGACAACTTGCTTTGC+AGG - chr4_3:5406064-5406083 Msa0626020:intron 35.0%
GCAGTTTCACTTAATCAATG+AGG - chr4_3:5406281-5406300 Msa0626020:CDS 35.0%
CTTCTCATCTATGGTTTCAT+TGG + chr4_3:5406671-5406690 None:intergenic 35.0%
!! CTTTTGTTGCTTCTCATCTA+TGG + chr4_3:5406680-5406699 None:intergenic 35.0%
GAGAATGATGAGTCATTTAG+AGG - chr4_3:5406876-5406895 Msa0626020:five_prime_UTR 35.0%
AGGAATGAATGATCCAGATT+GGG - chr4_3:5406896-5406915 Msa0626020:five_prime_UTR 35.0%
CCTCAACTTCAATTATGTTG+AGG + chr4_3:5407132-5407151 None:intergenic 35.0%
CCTCAACATAATTGAAGTTG+AGG - chr4_3:5407129-5407148 Msa0626020:five_prime_UTR 35.0%
GTCACGTTTGCATCAATCAA+TGG - chr4_3:5405317-5405336 Msa0626020:three_prime_UTR 40.0%
TAGAATTGGAACAATGGGCA+CGG + chr4_3:5405566-5405585 None:intergenic 40.0%
! CAAAGAACCAAACTTTGAGG+TGG + chr4_3:5405617-5405636 None:intergenic 40.0%
!! ACTACTGCTGTTGGTGTTAT+TGG - chr4_3:5405876-5405895 Msa0626020:intron 40.0%
!! GTTGGTGTTATTGGTGGTAA+TGG - chr4_3:5405885-5405904 Msa0626020:intron 40.0%
GGATCTTGTGTTTCATGGAT+TGG - chr4_3:5405906-5405925 Msa0626020:intron 40.0%
TGTGCAAAGGAAGAGATTGT+GGG - chr4_3:5406256-5406275 Msa0626020:CDS 40.0%
GAGGAATGAATGATCCAGAT+TGG - chr4_3:5406895-5406914 Msa0626020:five_prime_UTR 40.0%
GGAATGAATGATCCAGATTG+GGG - chr4_3:5406897-5406916 Msa0626020:five_prime_UTR 40.0%
AGCACCAACCAAATTTGGAT+GGG + chr4_3:5407072-5407091 None:intergenic 40.0%
!! CAAAGAGCACCAACCAAATT+TGG + chr4_3:5407077-5407096 None:intergenic 40.0%
GTCACGTTTGCATCAATCAA+TGG - chr4_3:5405317-5405336 Msa0626020:three_prime_UTR 40.0%
TAGAATTGGAACAATGGGCA+CGG + chr4_3:5405566-5405585 None:intergenic 40.0%
! CAAAGAACCAAACTTTGAGG+TGG + chr4_3:5405617-5405636 None:intergenic 40.0%
!! ACTACTGCTGTTGGTGTTAT+TGG - chr4_3:5405876-5405895 Msa0626020:intron 40.0%
!! GTTGGTGTTATTGGTGGTAA+TGG - chr4_3:5405885-5405904 Msa0626020:intron 40.0%
GGATCTTGTGTTTCATGGAT+TGG - chr4_3:5405906-5405925 Msa0626020:intron 40.0%
TGTGCAAAGGAAGAGATTGT+GGG - chr4_3:5406256-5406275 Msa0626020:CDS 40.0%
GAGGAATGAATGATCCAGAT+TGG - chr4_3:5406895-5406914 Msa0626020:five_prime_UTR 40.0%
GGAATGAATGATCCAGATTG+GGG - chr4_3:5406897-5406916 Msa0626020:five_prime_UTR 40.0%
AGCACCAACCAAATTTGGAT+GGG + chr4_3:5407072-5407091 None:intergenic 40.0%
!! CAAAGAGCACCAACCAAATT+TGG + chr4_3:5407077-5407096 None:intergenic 40.0%
TGATTGATGCAAACGTGACG+TGG + chr4_3:5405316-5405335 None:intergenic 45.0%
!! ACTGCTGTTGGTGTTATTGG+TGG - chr4_3:5405879-5405898 Msa0626020:intron 45.0%
!! ACATCAGATTCAGAAGCACC+AGG + chr4_3:5406035-5406054 None:intergenic 45.0%
TACCATCCAGATGTGTGCAA+AGG - chr4_3:5406243-5406262 Msa0626020:CDS 45.0%
TTCCTTTGCACACATCTGGA+TGG + chr4_3:5406248-5406267 None:intergenic 45.0%
TCTCTTCCTTTGCACACATC+TGG + chr4_3:5406252-5406271 None:intergenic 45.0%
GTGTGCAAAGGAAGAGATTG+TGG - chr4_3:5406255-5406274 Msa0626020:CDS 45.0%
GTGCAAAGGAAGAGATTGTG+GGG - chr4_3:5406257-5406276 Msa0626020:CDS 45.0%
ATGATCCAGATTGGGGCTAT+TGG - chr4_3:5406904-5406923 Msa0626020:five_prime_UTR 45.0%
TGATCCAGATTGGGGCTATT+GGG - chr4_3:5406905-5406924 Msa0626020:five_prime_UTR 45.0%
ATTGGGGCTATTGGGAAGAA+TGG - chr4_3:5406913-5406932 Msa0626020:five_prime_UTR 45.0%
GGCTATTGGGAAGAATGGAT+GGG - chr4_3:5406918-5406937 Msa0626020:five_prime_UTR 45.0%
ATTGGGAAGAATGGATGGGT+TGG - chr4_3:5406922-5406941 Msa0626020:five_prime_UTR 45.0%
! TTGGGAAGAATGGATGGGTT+GGG - chr4_3:5406923-5406942 Msa0626020:five_prime_UTR 45.0%
ACTTGTCCCCCATCCAAATT+TGG - chr4_3:5407061-5407080 Msa0626020:five_prime_UTR 45.0%
CACCAACCAAATTTGGATGG+GGG + chr4_3:5407070-5407089 None:intergenic 45.0%
GCACCAACCAAATTTGGATG+GGG + chr4_3:5407071-5407090 None:intergenic 45.0%
GAGCACCAACCAAATTTGGA+TGG + chr4_3:5407073-5407092 None:intergenic 45.0%
TGATTGATGCAAACGTGACG+TGG + chr4_3:5405316-5405335 None:intergenic 45.0%
!! ACTGCTGTTGGTGTTATTGG+TGG - chr4_3:5405879-5405898 Msa0626020:intron 45.0%
!! ACATCAGATTCAGAAGCACC+AGG + chr4_3:5406035-5406054 None:intergenic 45.0%
TACCATCCAGATGTGTGCAA+AGG - chr4_3:5406243-5406262 Msa0626020:CDS 45.0%
TTCCTTTGCACACATCTGGA+TGG + chr4_3:5406248-5406267 None:intergenic 45.0%
TCTCTTCCTTTGCACACATC+TGG + chr4_3:5406252-5406271 None:intergenic 45.0%
GTGTGCAAAGGAAGAGATTG+TGG - chr4_3:5406255-5406274 Msa0626020:CDS 45.0%
GTGCAAAGGAAGAGATTGTG+GGG - chr4_3:5406257-5406276 Msa0626020:CDS 45.0%
ATGATCCAGATTGGGGCTAT+TGG - chr4_3:5406904-5406923 Msa0626020:five_prime_UTR 45.0%
TGATCCAGATTGGGGCTATT+GGG - chr4_3:5406905-5406924 Msa0626020:five_prime_UTR 45.0%
ATTGGGGCTATTGGGAAGAA+TGG - chr4_3:5406913-5406932 Msa0626020:five_prime_UTR 45.0%
GGCTATTGGGAAGAATGGAT+GGG - chr4_3:5406918-5406937 Msa0626020:five_prime_UTR 45.0%
ATTGGGAAGAATGGATGGGT+TGG - chr4_3:5406922-5406941 Msa0626020:five_prime_UTR 45.0%
! TTGGGAAGAATGGATGGGTT+GGG - chr4_3:5406923-5406942 Msa0626020:five_prime_UTR 45.0%
ACTTGTCCCCCATCCAAATT+TGG - chr4_3:5407061-5407080 Msa0626020:five_prime_UTR 45.0%
CACCAACCAAATTTGGATGG+GGG + chr4_3:5407070-5407089 None:intergenic 45.0%
GCACCAACCAAATTTGGATG+GGG + chr4_3:5407071-5407090 None:intergenic 45.0%
GAGCACCAACCAAATTTGGA+TGG + chr4_3:5407073-5407092 None:intergenic 45.0%
!!! TTTTGATTTTTTTTAAAAAT+AGG + chr4_3:5405484-5405503 None:intergenic 5.0%
!!! TTTTGATTTTTTTTAAAAAT+AGG + chr4_3:5405484-5405503 None:intergenic 5.0%
ATGGCTGCTACTACTGCTGT+TGG - chr4_3:5405867-5405886 Msa0626020:intron 50.0%
TCTTCCCAATAGCCCCAATC+TGG + chr4_3:5406912-5406931 None:intergenic 50.0%
GGGCTATTGGGAAGAATGGA+TGG - chr4_3:5406917-5406936 Msa0626020:five_prime_UTR 50.0%
!! GAAGAATGGATGGGTTGGGA+AGG - chr4_3:5406927-5406946 Msa0626020:five_prime_UTR 50.0%
TTGGATGGGGGACAAGTAGT+AGG + chr4_3:5407058-5407077 None:intergenic 50.0%
GTCCCCCATCCAAATTTGGT+TGG - chr4_3:5407065-5407084 Msa0626020:five_prime_UTR 50.0%
GCAAACGTGACGTGGAATCT+CGG + chr4_3:5405308-5405327 None:intergenic 50.0%
ATGGCTGCTACTACTGCTGT+TGG - chr4_3:5405867-5405886 Msa0626020:intron 50.0%
TCTTCCCAATAGCCCCAATC+TGG + chr4_3:5406912-5406931 None:intergenic 50.0%
GGGCTATTGGGAAGAATGGA+TGG - chr4_3:5406917-5406936 Msa0626020:five_prime_UTR 50.0%
!! GAAGAATGGATGGGTTGGGA+AGG - chr4_3:5406927-5406946 Msa0626020:five_prime_UTR 50.0%
TTGGATGGGGGACAAGTAGT+AGG + chr4_3:5407058-5407077 None:intergenic 50.0%
GTCCCCCATCCAAATTTGGT+TGG - chr4_3:5407065-5407084 Msa0626020:five_prime_UTR 50.0%
!! TGGATGGGTTGGGAAGGAGC+AGG - chr4_3:5406933-5406952 Msa0626020:five_prime_UTR 60.0%
!! TGGATGGGTTGGGAAGGAGC+AGG - chr4_3:5406933-5406952 Msa0626020:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 5405306 5407246 5405306 ID=Msa0626020;Name=Msa0626020
chr4_3 mRNA 5405306 5407246 5405306 ID=Msa0626020-mRNA-1;Parent=Msa0626020;Name=Msa0626020-mRNA-1;_AED=0.14;_eAED=0.14;_QI=561|1|1|1|1|1|3|251|156
chr4_3 exon 5405306 5405739 5405306 ID=Msa0626020-mRNA-1:exon:1512;Parent=Msa0626020-mRNA-1
chr4_3 exon 5406241 5406309 5406241 ID=Msa0626020-mRNA-1:exon:1511;Parent=Msa0626020-mRNA-1
chr4_3 exon 5406467 5407246 5406467 ID=Msa0626020-mRNA-1:exon:1510;Parent=Msa0626020-mRNA-1
chr4_3 five_prime_UTR 5406686 5407246 5406686 ID=Msa0626020-mRNA-1:five_prime_utr;Parent=Msa0626020-mRNA-1
chr4_3 CDS 5406467 5406685 5406467 ID=Msa0626020-mRNA-1:cds;Parent=Msa0626020-mRNA-1
chr4_3 CDS 5406241 5406309 5406241 ID=Msa0626020-mRNA-1:cds;Parent=Msa0626020-mRNA-1
chr4_3 CDS 5405557 5405739 5405557 ID=Msa0626020-mRNA-1:cds;Parent=Msa0626020-mRNA-1
chr4_3 three_prime_UTR 5405306 5405556 5405306 ID=Msa0626020-mRNA-1:three_prime_utr;Parent=Msa0626020-mRNA-1
Gene Sequence

>Msa0626020

ATGGCTGCTACTACTGCTGTTGGTGTTATTGGTGGTAATGGATCTTGTGTTTCATGGATTGGAAGAAAGGAAAAGAAACAAAATAAGATGAATAAAGTTAGGGTTTGTTGCTCTAAATCTAAATCTTCTTCTGTGATGGATCCTTATAAGACTTTGAGAATTCAACCTGGTGCTTCTGAATCTGATGTTAGAAAGGCTTTTAGACAACTTGCTTTGCAGTACCATCCAGATGTGTGCAAAGGAAGAGATTGTGGGGTGCAGTTTCACTTAATCAATGAGGCTTATGATATTGTGATGTCCAATTTGAGAGAAAATGTGAAAGAAACTTATGAGACGACCACATATAATGAAGAGAATGATGAGTCATTTAGAGGAATGAATGATCCAGATTGGGGCTATTGGGAAGAATGGATGGGTTGGGAAGGAGCAGGAATCCGTGACTACTCTTCTCATATTAATCCTTACATTTAA
Protein sequence

>Msa0626020

MAATTAVGVIGGNGSCVSWIGRKEKKQNKMNKVRVCCSKSKSSSVMDPYKTLRIQPGASESDVRKAFRQLALQYHPDVCKGRDCGVQFHLINEAYDIVMSNLRENVKETYETTTYNEENDESFRGMNDPDWGYWEEWMGWEGAGIRDYSSHINPYI*