AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605590 MtrunA17_Chr4g0034951 98.392 311 4 1 1 310 1 311 0.0 629
Msa0605590 MtrunA17_Chr1g0155081 49.320 294 127 7 26 309 30 311 5.33e-91 273
Msa0605590 MtrunA17_Chr8g0348371 41.853 313 154 9 14 310 3 303 3.68e-66 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605590 AT1G19400.1 54.485 301 127 5 13 310 10 303 1.17e-107 316
Msa0605590 AT1G19400.3 54.485 301 127 5 13 310 10 303 1.17e-107 316
Msa0605590 AT1G19400.2 54.485 301 127 5 13 310 10 303 1.17e-107 316
Msa0605590 AT1G75180.1 56.934 274 114 3 41 310 42 315 5.72e-107 314
Msa0605590 AT1G75180.3 56.934 274 114 3 41 310 42 315 5.72e-107 314
Msa0605590 AT1G19400.4 54.185 227 98 3 13 236 10 233 7.81e-79 240
Msa0605590 AT1G75180.2 56.477 193 82 1 41 231 42 234 1.37e-72 224
Msa0605590 AT1G75180.4 56.477 193 82 1 41 231 42 234 1.53e-71 223
Msa0605590 AT1G01500.1 41.892 296 141 10 36 310 42 327 1.13e-63 204

Find 131 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGGCACAAAGTTCTTTA+AGG 0.155540 4_2:-51530881 Msa0605590:CDS
AGGTGGTAATAAATGATTTA+AGG 0.188726 4_2:-51529468 Msa0605590:three_prime_UTR
ATGGATATTGTTTAATTTAA+TGG 0.209398 4_2:+51532333 None:intergenic
GATACCCTTCTTGAAGTTAA+TGG 0.212761 4_2:-51531156 Msa0605590:CDS
GAGAATGATGCAAATGAAAA+TGG 0.217056 4_2:-51530706 Msa0605590:CDS
TCCTTCTGCGCTTGCTTTGC+AGG 0.233001 4_2:-51530677 Msa0605590:intron
CTTTAAGCTTCAATAGATTC+TGG 0.234279 4_2:-51532233 Msa0605590:five_prime_UTR
AGACATTAACATGTTGTCTT+TGG 0.250262 4_2:+51529636 None:intergenic
CATGGTTTAACGCCGGTGTT+AGG 0.255714 4_2:-51529855 Msa0605590:CDS
AAGAACAAATCTTGCAATCT+TGG 0.266859 4_2:+51532253 None:intergenic
GAGAATTCGTATAAGGTTAA+TGG 0.283457 4_2:-51531357 Msa0605590:CDS
GGAAATGGACCAAGTGAAAA+TGG 0.283915 4_2:-51531336 Msa0605590:CDS
GTTAGGGTTGGTGTTGGTAT+TGG 0.297925 4_2:-51529838 Msa0605590:CDS
ATTGCTGTTACATAATTCTA+AGG 0.316159 4_2:+51530969 None:intergenic
GGATATATATTATATAGTTT+TGG 0.316791 4_2:-51529596 Msa0605590:three_prime_UTR
CTCTTATCAACCCTCGATAA+AGG 0.329977 4_2:-51531289 Msa0605590:CDS
AGAAGGGTATCAGGATTTAA+AGG 0.331368 4_2:+51531168 None:intergenic
GGAGAAGCAATTGTTGTTGC+CGG 0.344534 4_2:-51530860 Msa0605590:CDS
GACATTAACATGTTGTCTTT+GGG 0.346532 4_2:+51529637 None:intergenic
AAAGATAAGCTTCTTCTGTT+TGG 0.350138 4_2:-51530994 Msa0605590:CDS
TTAACTTCAAGAAGGGTATC+AGG 0.353127 4_2:+51531159 None:intergenic
ATCAAAACATAGGTTGTTAT+CGG 0.360179 4_2:+51532281 None:intergenic
TCGTATAAGGTTAATGGAAA+TGG 0.363071 4_2:-51531351 Msa0605590:CDS
GCGAAGATGGGGAACTTTCA+TGG 0.371984 4_2:-51529873 Msa0605590:CDS
AGAATAGCCGTCTTCTGAAT+TGG 0.372025 4_2:+51529746 None:intergenic
AGAACAAATCTTGCAATCTT+GGG 0.374948 4_2:+51532254 None:intergenic
GTTAGAGAATCAAATTCTAA+TGG 0.378979 4_2:-51530940 Msa0605590:CDS
TATATTGCCGGGTCCTTCTC+TGG 0.379377 4_2:-51530811 Msa0605590:CDS
TAGTTCGGCCATCTTTATCT+TGG 0.380101 4_2:-51529660 Msa0605590:three_prime_UTR
CGTTACCGTGGTTAGACATA+AGG 0.381604 4_2:-51531263 Msa0605590:CDS
TGCGATGGAGAATTCGTATA+AGG 0.386471 4_2:-51531364 Msa0605590:exon
TGATCAACATTTATAAAGTA+AGG 0.389780 4_2:-51529336 Msa0605590:three_prime_UTR
ACAATTGAGGTCTATATTGC+CGG 0.393367 4_2:-51530823 Msa0605590:CDS
TTGTAAGCACTGATAGTATA+AGG 0.394406 4_2:-51531050 Msa0605590:CDS
TTTAAGCTTCAATAGATTCT+GGG 0.398434 4_2:-51532232 Msa0605590:five_prime_UTR
TGTTTGTCATACATGTAATC+TGG 0.402010 4_2:+51529946 None:intergenic
GAAAACTGAAATGTTATGTT+GGG 0.402948 4_2:+51532485 None:intergenic
GGTGGTAATAAATGATTTAA+GGG 0.404373 4_2:-51529467 Msa0605590:three_prime_UTR
TACTTTATAAATGTTGATCA+TGG 0.417685 4_2:+51529339 None:intergenic
TTTGTGTGTGAATTAATTAG+AGG 0.422085 4_2:+51532428 None:intergenic
TTCTTGAAGTTAATGGTGTT+CGG 0.422504 4_2:-51531149 Msa0605590:CDS
CTTAGTGTTTGTCTTGGAAT+CGG 0.423405 4_2:-51529814 Msa0605590:CDS
TGATTAAGTGTGAGAATCTC+AGG 0.428692 4_2:+51531195 None:intergenic
CGGAGTAGTTTATATTCTGA+TGG 0.435949 4_2:-51531129 Msa0605590:CDS
GCAATATAGACCTCAATTGT+TGG 0.437528 4_2:+51530826 None:intergenic
TTATATTCCCCTACTTATTG+AGG 0.438498 4_2:-51529488 Msa0605590:three_prime_UTR
CTTGGAATCGGCATTGGAGT+TGG 0.441010 4_2:-51529802 Msa0605590:CDS
TTATTACCACCTCAATAAGT+AGG 0.441077 4_2:+51529479 None:intergenic
GGATTCCAACCCTTCTCAAA+AGG 0.443968 4_2:-51531107 Msa0605590:CDS
TCCTTAAAGAACTTTGTGCC+TGG 0.447833 4_2:+51530880 None:intergenic
GGATAGGGTGGATAGAAAAT+CGG 0.450168 4_2:-51531085 Msa0605590:CDS
GTTTGTCTTGGAATCGGCAT+TGG 0.451016 4_2:-51529808 Msa0605590:CDS
TGTGAGTCGAATATAATTCC+AGG 0.452189 4_2:-51530898 Msa0605590:CDS
TGAAAACTGAAATGTTATGT+TGG 0.452656 4_2:+51532484 None:intergenic
TCTTATCAACCCTCGATAAA+GGG 0.453924 4_2:-51531288 Msa0605590:CDS
AAGAATAATATCAGATTCAA+AGG 0.458806 4_2:-51532368 Msa0605590:five_prime_UTR
ATGGTTTAACGCCGGTGTTA+GGG 0.458989 4_2:-51529854 Msa0605590:CDS
GAATTAATTAGAGGAAGAAA+AGG 0.460023 4_2:+51532437 None:intergenic
AGAATGATGCAAATGAAAAT+GGG 0.470852 4_2:-51530705 Msa0605590:CDS
CGTATGCTGTTCTTGTGTAT+AGG 0.473133 4_2:+51529902 None:intergenic
TGAATTGGCCGGTGGCACCT+TGG 0.475731 4_2:+51529761 None:intergenic
CAACCCTTCTCAAAAGGGAT+AGG 0.480345 4_2:-51531101 Msa0605590:CDS
ATTGGACTTAGTGTTTGTCT+TGG 0.481547 4_2:-51529820 Msa0605590:CDS
TATTACCACCTCAATAAGTA+GGG 0.486522 4_2:+51529480 None:intergenic
GCATACGCTGACGGCGAAGA+TGG 0.492307 4_2:-51529886 Msa0605590:CDS
CAAATTCTAATGGACAAAGT+TGG 0.492533 4_2:-51530930 Msa0605590:CDS
GTTTAATTTAATGGAAGATG+TGG 0.493650 4_2:+51532342 None:intergenic
GTGGATTAGTGTTGCTGTGT+TGG 0.494724 4_2:-51531448 Msa0605590:five_prime_UTR
AGATAAAGATGGCCGAACTA+AGG 0.499057 4_2:+51529663 None:intergenic
GTTACCGTGGTTAGACATAA+GGG 0.505953 4_2:-51531262 Msa0605590:CDS
CATACGCTGACGGCGAAGAT+GGG 0.511131 4_2:-51529885 Msa0605590:CDS
GATTCCAACCCTTCTCAAAA+GGG 0.526688 4_2:-51531106 Msa0605590:CDS
GGAGTAGTTTATATTCTGAT+GGG 0.528712 4_2:-51531128 Msa0605590:CDS
GATCAACATTTATAAAGTAA+GGG 0.530296 4_2:-51529335 Msa0605590:three_prime_UTR
ACCTGCAAAGCAAGCGCAGA+AGG 0.534939 4_2:+51530676 None:intergenic
AGAATAATATCAGATTCAAA+GGG 0.538270 4_2:-51532367 Msa0605590:five_prime_UTR
AAAGGAGAGTGTGAAATTGT+TGG 0.539146 4_2:+51532455 None:intergenic
GGTTGTTATCGGTTATGTTG+AGG 0.539393 4_2:+51532292 None:intergenic
CAAAGAAACTGAGAAAATCA+TGG 0.543052 4_2:+51529394 None:intergenic
AACCCTTCTCAAAAGGGATA+GGG 0.548703 4_2:-51531100 Msa0605590:CDS
GAGTGGTAGTGTGAAGTTTG+AGG 0.549063 4_2:-51531025 Msa0605590:CDS
GCCGGTGTTAGGGTTGGTGT+TGG 0.549235 4_2:-51529844 Msa0605590:CDS
AATCATGGTGGAGAGACTAA+TGG 0.550185 4_2:+51529409 None:intergenic
CTTGGGATGAATCAAAACAT+AGG 0.551006 4_2:+51532271 None:intergenic
CTCTACTCTTCCAACAATTG+AGG 0.556408 4_2:-51530836 Msa0605590:CDS
TATTCCCCTACTTATTGAGG+TGG 0.561142 4_2:-51529485 Msa0605590:three_prime_UTR
TGAGGTGGTAAGAGAAGATG+TGG 0.564391 4_2:+51532310 None:intergenic
GTGGTAATAAATGATTTAAG+GGG 0.565067 4_2:-51529466 Msa0605590:three_prime_UTR
AAATCATACCAAGGTGCCAC+CGG 0.568352 4_2:-51529769 Msa0605590:CDS
AAAGTAGAACTGTCTTGCGA+TGG 0.568506 4_2:-51531379 Msa0605590:exon
GAACACCATTAACTTCAAGA+AGG 0.569037 4_2:+51531151 None:intergenic
TTTAACGCCGGTGTTAGGGT+TGG 0.570434 4_2:-51529850 Msa0605590:CDS
TGTTATCGGTTATGTTGAGG+TGG 0.575774 4_2:+51532295 None:intergenic
TTTAATTTAATGGAAGATGT+GGG 0.578479 4_2:+51532343 None:intergenic
TAGCCGTCTTCTGAATTGGC+CGG 0.581948 4_2:+51529750 None:intergenic
CAATTGAGGTCTATATTGCC+GGG 0.582339 4_2:-51530822 Msa0605590:CDS
ATCAACATTTATAAAGTAAG+GGG 0.586146 4_2:-51529334 Msa0605590:three_prime_UTR
CCACCGGCCAATTCAGAAGA+CGG 0.588469 4_2:-51529753 Msa0605590:CDS
TCTGGAATTGCATTCAACGC+CGG 0.588676 4_2:+51530727 None:intergenic
AAAATGGATACTCTATTGCT+AGG 0.589815 4_2:-51531320 Msa0605590:CDS
CGATAAAGGGTTCGTTACCG+TGG 0.592561 4_2:-51531275 Msa0605590:CDS
AAGGTCTTCGTTAAGATGAT+CGG 0.592566 4_2:+51530784 None:intergenic
CGGAGTGCCAGAGAAGGACC+CGG 0.592882 4_2:+51530804 None:intergenic
GATGCAAATGAAAATGGGAA+AGG 0.596141 4_2:-51530700 Msa0605590:CDS
AACACCATTAACTTCAAGAA+GGG 0.596194 4_2:+51531152 None:intergenic
CCTTCTCAAAAGGGATAGGG+TGG 0.600087 4_2:-51531097 Msa0605590:CDS
ATTGTTGGAAGAGTAGAGTC+CGG 0.603903 4_2:+51530841 None:intergenic
TGGTAATAAATGATTTAAGG+GGG 0.603940 4_2:-51529465 Msa0605590:three_prime_UTR
GTGGTAAGAGAAGATGTGGA+TGG 0.604928 4_2:+51532314 None:intergenic
ACAAGAACAGCATACGCTGA+CGG 0.606360 4_2:-51529895 Msa0605590:CDS
GCTGTGTTGGTACAAGTCAA+TGG 0.607038 4_2:-51531435 Msa0605590:five_prime_UTR
TCTTTGGGCCAAGATAAAGA+TGG 0.610464 4_2:+51529652 None:intergenic
ACCAACACCAACCCTAACAC+CGG 0.618909 4_2:+51529843 None:intergenic
AAAACTGAAATGTTATGTTG+GGG 0.627111 4_2:+51532486 None:intergenic
CGGTAACGAACCCTTTATCG+AGG 0.628590 4_2:+51531278 None:intergenic
ATACGCTGACGGCGAAGATG+GGG 0.629010 4_2:-51529884 Msa0605590:CDS
ACTGATAGTATAAGGATGAG+TGG 0.634943 4_2:-51531042 Msa0605590:CDS
CATGATGATGAGGATTATCA+CGG 0.635768 4_2:-51529925 Msa0605590:CDS
GATGATCGGAGTGCCAGAGA+AGG 0.637274 4_2:+51530798 None:intergenic
CCGTCTTCTGAATTGGCCGG+TGG 0.642624 4_2:+51529753 None:intergenic
GGTAACGAACCCTTTATCGA+GGG 0.642976 4_2:+51531279 None:intergenic
AACACCCTTATGTCTAACCA+CGG 0.647619 4_2:+51531258 None:intergenic
TTAGAATTATGTAACAGCAA+TGG 0.648523 4_2:-51530967 Msa0605590:CDS
GAACTTTCATGGTTTAACGC+CGG 0.650464 4_2:-51529862 Msa0605590:CDS
ATTACCACCTCAATAAGTAG+GGG 0.652127 4_2:+51529481 None:intergenic
AAGTGTGAGAATCTCAGGAG+TGG 0.662685 4_2:+51531200 None:intergenic
GTATGACAAACATGATGATG+AGG 0.669080 4_2:-51529935 Msa0605590:CDS
GTCTTGTTCATCGAGTGCAA+AGG 0.669479 4_2:-51529617 Msa0605590:three_prime_UTR
TGATTCTTACAGTAATCACA+AGG 0.698127 4_2:-51529434 Msa0605590:three_prime_UTR
ATACTCGTTAAATCATACCA+AGG 0.711189 4_2:-51529778 Msa0605590:CDS
AGAAACTGAGAAAATCATGG+TGG 0.715746 4_2:+51529397 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAATTTGATTTTTAATTT+TGG - chr4_2:51529702-51529721 Msa0605590:three_prime_UTR 10.0%
!!! ATGGATATTGTTTAATTTAA+TGG + chr4_2:51529523-51529542 None:intergenic 15.0%
!!! AAATTTTGATTATTGTTATG+TGG + chr4_2:51529690-51529709 None:intergenic 15.0%
!! CAAAGAAATATAAGAATTAA+AGG + chr4_2:51530087-51530106 None:intergenic 15.0%
!! TTTACATAAGAAAATCAAAA+AGG + chr4_2:51530354-51530373 None:intergenic 15.0%
!! AATATAACTGTAAATTATCT+TGG - chr4_2:51531620-51531639 Msa0605590:intron 15.0%
!!! AAATTTTTAAAATCTTGTCA+AGG + chr4_2:51531790-51531809 None:intergenic 15.0%
!! AGCATTTACATAAAATAATA+AGG + chr4_2:51531880-51531899 None:intergenic 15.0%
!!! GGATATATATTATATAGTTT+TGG - chr4_2:51532257-51532276 Msa0605590:five_prime_UTR 15.0%
!! AAGAATAATATCAGATTCAA+AGG - chr4_2:51529485-51529504 Msa0605590:three_prime_UTR 20.0%
!! AGAATAATATCAGATTCAAA+GGG - chr4_2:51529486-51529505 Msa0605590:three_prime_UTR 20.0%
!!! TTTAATTTAATGGAAGATGT+GGG + chr4_2:51529513-51529532 None:intergenic 20.0%
!!! TTGAATTTTGCATTAGTTAA+TGG - chr4_2:51529770-51529789 Msa0605590:CDS 20.0%
!! CAATCAGATAAAAATAATAC+AGG + chr4_2:51530156-51530175 None:intergenic 20.0%
!!! TATTTTTATCTGATTGAGTA+AGG - chr4_2:51530160-51530179 Msa0605590:intron 20.0%
!!! ATTTATTTGTGCTTTTATGT+TGG - chr4_2:51530285-51530304 Msa0605590:intron 20.0%
!! ATTTAAATAAGCACATGAAA+AGG + chr4_2:51531225-51531244 None:intergenic 20.0%
!!! GGAATTATCTATAATTTGTT+GGG + chr4_2:51531859-51531878 None:intergenic 20.0%
!!! AGGAATTATCTATAATTTGT+TGG + chr4_2:51531860-51531879 None:intergenic 20.0%
!! TACTTTATAAATGTTGATCA+TGG + chr4_2:51532517-51532536 None:intergenic 20.0%
!! TGATCAACATTTATAAAGTA+AGG - chr4_2:51532517-51532536 Msa0605590:five_prime_UTR 20.0%
!! GATCAACATTTATAAAGTAA+GGG - chr4_2:51532518-51532537 Msa0605590:five_prime_UTR 20.0%
!! ATCAACATTTATAAAGTAAG+GGG - chr4_2:51532519-51532538 Msa0605590:five_prime_UTR 20.0%
! AAAACTGAAATGTTATGTTG+GGG + chr4_2:51529370-51529389 None:intergenic 25.0%
! GAAAACTGAAATGTTATGTT+GGG + chr4_2:51529371-51529390 None:intergenic 25.0%
! TGAAAACTGAAATGTTATGT+TGG + chr4_2:51529372-51529391 None:intergenic 25.0%
! GAATTAATTAGAGGAAGAAA+AGG + chr4_2:51529419-51529438 None:intergenic 25.0%
! TTTGTGTGTGAATTAATTAG+AGG + chr4_2:51529428-51529447 None:intergenic 25.0%
!! GTTTAATTTAATGGAAGATG+TGG + chr4_2:51529514-51529533 None:intergenic 25.0%
! ATCAAAACATAGGTTGTTAT+CGG + chr4_2:51529575-51529594 None:intergenic 25.0%
! TTTAAGCTTCAATAGATTCT+GGG - chr4_2:51529621-51529640 Msa0605590:three_prime_UTR 25.0%
! ATTTAGACAAAAAAAACAGC+CGG + chr4_2:51529857-51529876 None:intergenic 25.0%
! GAAAATCAAAAAGGTTGAAA+AGG + chr4_2:51530345-51530364 None:intergenic 25.0%
!! ATTGCTGTTACATAATTCTA+AGG + chr4_2:51530887-51530906 None:intergenic 25.0%
! TTAGAATTATGTAACAGCAA+TGG - chr4_2:51530886-51530905 Msa0605590:CDS 25.0%
! GTTAGAGAATCAAATTCTAA+TGG - chr4_2:51530913-51530932 Msa0605590:CDS 25.0%
! AGAATGATGCAAATGAAAAT+GGG - chr4_2:51531148-51531167 Msa0605590:CDS 25.0%
!! TACAAAGTGTTCTTAAATAG+TGG - chr4_2:51531395-51531414 Msa0605590:five_prime_UTR 25.0%
! ATATTTATGACCAAACCAAA+TGG - chr4_2:51531810-51531829 Msa0605590:intron 25.0%
! TTCTTCATAAGATTTCCATT+TGG + chr4_2:51531828-51531847 None:intergenic 25.0%
!!! TCTATAATTTGTTGGGAAAA+TGG + chr4_2:51531852-51531871 None:intergenic 25.0%
!!! TTATTTTATGTAAATGCTGC+AGG - chr4_2:51531882-51531901 Msa0605590:intron 25.0%
! AGGTGGTAATAAATGATTTA+AGG - chr4_2:51532385-51532404 Msa0605590:five_prime_UTR 25.0%
! GGTGGTAATAAATGATTTAA+GGG - chr4_2:51532386-51532405 Msa0605590:five_prime_UTR 25.0%
! GTGGTAATAAATGATTTAAG+GGG - chr4_2:51532387-51532406 Msa0605590:five_prime_UTR 25.0%
! TGGTAATAAATGATTTAAGG+GGG - chr4_2:51532388-51532407 Msa0605590:five_prime_UTR 25.0%
!!! AACATTTTTTGTCTGAGAAA+AGG + chr4_2:51532485-51532504 None:intergenic 25.0%
!!! TTTTCTGTTTGCTGTATAAG+GGG + chr4_2:51529347-51529366 None:intergenic 30.0%
!!! GTTTTCTGTTTGCTGTATAA+GGG + chr4_2:51529348-51529367 None:intergenic 30.0%
AGAACAAATCTTGCAATCTT+GGG + chr4_2:51529602-51529621 None:intergenic 30.0%
AAGAACAAATCTTGCAATCT+TGG + chr4_2:51529603-51529622 None:intergenic 30.0%
CTTTAAGCTTCAATAGATTC+TGG - chr4_2:51529620-51529639 Msa0605590:three_prime_UTR 30.0%
! TTTTGGCACAAGATATTTTG+TGG - chr4_2:51529719-51529738 Msa0605590:three_prime_UTR 30.0%
CCTCAAACATATCAACTATT+CGG + chr4_2:51529969-51529988 None:intergenic 30.0%
! TATTTGTGCTTTTATGTTGG+TGG - chr4_2:51530288-51530307 Msa0605590:intron 30.0%
GAGAATTCGTATAAGGTTAA+TGG - chr4_2:51530496-51530515 Msa0605590:intron 30.0%
TCGTATAAGGTTAATGGAAA+TGG - chr4_2:51530502-51530521 Msa0605590:intron 30.0%
!! ATAGAGTATCCATTTTCACT+TGG + chr4_2:51530529-51530548 None:intergenic 30.0%
AAAATGGATACTCTATTGCT+AGG - chr4_2:51530533-51530552 Msa0605590:intron 30.0%
!!! ACATAAGGGTGTTTTATGTA+AGG - chr4_2:51530605-51530624 Msa0605590:intron 30.0%
!!! CATAAGGGTGTTTTATGTAA+GGG - chr4_2:51530606-51530625 Msa0605590:intron 30.0%
AACACCATTAACTTCAAGAA+GGG + chr4_2:51530704-51530723 None:intergenic 30.0%
TTCTTGAAGTTAATGGTGTT+CGG - chr4_2:51530704-51530723 Msa0605590:CDS 30.0%
GGAGTAGTTTATATTCTGAT+GGG - chr4_2:51530725-51530744 Msa0605590:CDS 30.0%
!! TTGTAAGCACTGATAGTATA+AGG - chr4_2:51530803-51530822 Msa0605590:CDS 30.0%
AAAGATAAGCTTCTTCTGTT+TGG - chr4_2:51530859-51530878 Msa0605590:CDS 30.0%
! CAAATTCTAATGGACAAAGT+TGG - chr4_2:51530923-51530942 Msa0605590:CDS 30.0%
!! TTTTTGAGAACTAAGATGCA+AGG + chr4_2:51531091-51531110 None:intergenic 30.0%
GTTCTCAAAAAAGACATACA+AGG - chr4_2:51531100-51531119 Msa0605590:CDS 30.0%
! TTTTCATTTGCATCATTCTC+TGG + chr4_2:51531147-51531166 None:intergenic 30.0%
GAGAATGATGCAAATGAAAA+TGG - chr4_2:51531147-51531166 Msa0605590:CDS 30.0%
ATTCAAGCAACAAGTTAACA+AGG + chr4_2:51531277-51531296 None:intergenic 30.0%
! GATTTGTTCAAATCCCTAAA+GGG + chr4_2:51531348-51531367 None:intergenic 30.0%
!! ATTGGCTCAACGTAATTTTA+TGG - chr4_2:51531505-51531524 Msa0605590:intron 30.0%
CATAAGATTTCCATTTGGTT+TGG + chr4_2:51531823-51531842 None:intergenic 30.0%
TGTTTGTCATACATGTAATC+TGG + chr4_2:51531910-51531929 None:intergenic 30.0%
ATACTCGTTAAATCATACCA+AGG - chr4_2:51532075-51532094 Msa0605590:intron 30.0%
AAAAACAGCTAACCTTAGTT+CGG - chr4_2:51532178-51532197 Msa0605590:five_prime_UTR 30.0%
GACATTAACATGTTGTCTTT+GGG + chr4_2:51532219-51532238 None:intergenic 30.0%
AGACATTAACATGTTGTCTT+TGG + chr4_2:51532220-51532239 None:intergenic 30.0%
TTATATTCCCCTACTTATTG+AGG - chr4_2:51532365-51532384 Msa0605590:five_prime_UTR 30.0%
TATTACCACCTCAATAAGTA+GGG + chr4_2:51532376-51532395 None:intergenic 30.0%
TTATTACCACCTCAATAAGT+AGG + chr4_2:51532377-51532396 None:intergenic 30.0%
TGATTCTTACAGTAATCACA+AGG - chr4_2:51532419-51532438 Msa0605590:five_prime_UTR 30.0%
CAAAGAAACTGAGAAAATCA+TGG + chr4_2:51532462-51532481 None:intergenic 30.0%
!!! GGTTTTCTGTTTGCTGTATA+AGG + chr4_2:51529349-51529368 None:intergenic 35.0%
! AAAGGAGAGTGTGAAATTGT+TGG + chr4_2:51529401-51529420 None:intergenic 35.0%
CTTGGGATGAATCAAAACAT+AGG + chr4_2:51529585-51529604 None:intergenic 35.0%
! GTTAATGGATGTGTTAGTGA+CGG - chr4_2:51529785-51529804 Msa0605590:CDS 35.0%
CAAACATATCAACTATTCGG+TGG + chr4_2:51529966-51529985 None:intergenic 35.0%
! CCGAATAGTTGATATGTTTG+AGG - chr4_2:51529966-51529985 Msa0605590:intron 35.0%
CCTGAAAAACAGACAAAAAG+AGG + chr4_2:51530012-51530031 None:intergenic 35.0%
!! CCTCTTTTTGTCTGTTTTTC+AGG - chr4_2:51530009-51530028 Msa0605590:intron 35.0%
!!! TTTTGTCTGTTTTTCAGGTC+TGG - chr4_2:51530014-51530033 Msa0605590:intron 35.0%
!! AGTTAACTTTGTTGTTGCAG+TGG - chr4_2:51530386-51530405 Msa0605590:intron 35.0%
TCTTATCAACCCTCGATAAA+GGG - chr4_2:51530565-51530584 Msa0605590:intron 35.0%
TGATTAAGTGTGAGAATCTC+AGG + chr4_2:51530661-51530680 None:intergenic 35.0%
!! AGAAGGGTATCAGGATTTAA+AGG + chr4_2:51530688-51530707 None:intergenic 35.0%
!! TTAACTTCAAGAAGGGTATC+AGG + chr4_2:51530697-51530716 None:intergenic 35.0%
GATACCCTTCTTGAAGTTAA+TGG - chr4_2:51530697-51530716 Msa0605590:CDS 35.0%
GAACACCATTAACTTCAAGA+AGG + chr4_2:51530705-51530724 None:intergenic 35.0%
CGGAGTAGTTTATATTCTGA+TGG - chr4_2:51530724-51530743 Msa0605590:CDS 35.0%
ACTGATAGTATAAGGATGAG+TGG - chr4_2:51530811-51530830 Msa0605590:CDS 35.0%
TGTGAGTCGAATATAATTCC+AGG - chr4_2:51530955-51530974 Msa0605590:CDS 35.0%
GCAATATAGACCTCAATTGT+TGG + chr4_2:51531030-51531049 None:intergenic 35.0%
ACAATTGAGGTCTATATTGC+CGG - chr4_2:51531030-51531049 Msa0605590:CDS 35.0%
AAGGTCTTCGTTAAGATGAT+CGG + chr4_2:51531072-51531091 None:intergenic 35.0%
AAAAAGACATACAAGGAAGC+CGG - chr4_2:51531107-51531126 Msa0605590:CDS 35.0%
GATGCAAATGAAAATGGGAA+AGG - chr4_2:51531153-51531172 Msa0605590:CDS 35.0%
! ATGCACTGACATAGTTAAAG+AGG + chr4_2:51531251-51531270 None:intergenic 35.0%
! CGATTTGTTCAAATCCCTAA+AGG + chr4_2:51531349-51531368 None:intergenic 35.0%
TGATATGCGATTGACAATAC+AGG - chr4_2:51531467-51531486 Msa0605590:intron 35.0%
ATTATGTTCCATCTCCTACT+AGG + chr4_2:51531592-51531611 None:intergenic 35.0%
!! TTATCTTGGCTCTTTTTCTG+AGG - chr4_2:51531634-51531653 Msa0605590:intron 35.0%
! AACTATTTTCATCACTCGTC+AGG + chr4_2:51531766-51531785 None:intergenic 35.0%
! GTATGACAAACATGATGATG+AGG - chr4_2:51531918-51531937 Msa0605590:intron 35.0%
! CATGATGATGAGGATTATCA+CGG - chr4_2:51531928-51531947 Msa0605590:intron 35.0%
ATTGGACTTAGTGTTTGTCT+TGG - chr4_2:51532033-51532052 Msa0605590:intron 35.0%
CTTAGTGTTTGTCTTGGAAT+CGG - chr4_2:51532039-51532058 Msa0605590:intron 35.0%
!!! TAGCTGTTTTTGGATCAAGA+GGG + chr4_2:51532169-51532188 None:intergenic 35.0%
!!! TTAGCTGTTTTTGGATCAAG+AGG + chr4_2:51532170-51532189 None:intergenic 35.0%
!! GAACTAAGGTTAGCTGTTTT+TGG + chr4_2:51532179-51532198 None:intergenic 35.0%
ATTACCACCTCAATAAGTAG+GGG + chr4_2:51532375-51532394 None:intergenic 35.0%
ATAAATGATTTAAGGGGGCT+TGG - chr4_2:51532393-51532412 Msa0605590:five_prime_UTR 35.0%
AGAAACTGAGAAAATCATGG+TGG + chr4_2:51532459-51532478 None:intergenic 35.0%
! TCCTCTCTTTTTCTTTCTCG+TGG - chr4_2:51529452-51529471 Msa0605590:three_prime_UTR 40.0%
TCCACGAGAAAGAAAAAGAG+AGG + chr4_2:51529456-51529475 None:intergenic 40.0%
! TGTTATCGGTTATGTTGAGG+TGG + chr4_2:51529561-51529580 None:intergenic 40.0%
! GGTTGTTATCGGTTATGTTG+AGG + chr4_2:51529564-51529583 None:intergenic 40.0%
AAAGTAGAACTGTCTTGCGA+TGG - chr4_2:51530474-51530493 Msa0605590:intron 40.0%
TGCGATGGAGAATTCGTATA+AGG - chr4_2:51530489-51530508 Msa0605590:intron 40.0%
GGAAATGGACCAAGTGAAAA+TGG - chr4_2:51530517-51530536 Msa0605590:intron 40.0%
CTCTTATCAACCCTCGATAA+AGG - chr4_2:51530564-51530583 Msa0605590:intron 40.0%
GTTACCGTGGTTAGACATAA+GGG - chr4_2:51530591-51530610 Msa0605590:intron 40.0%
AACACCCTTATGTCTAACCA+CGG + chr4_2:51530598-51530617 None:intergenic 40.0%
GATTCCAACCCTTCTCAAAA+GGG - chr4_2:51530747-51530766 Msa0605590:CDS 40.0%
AACCCTTCTCAAAAGGGATA+GGG - chr4_2:51530753-51530772 Msa0605590:CDS 40.0%
GGATAGGGTGGATAGAAAAT+CGG - chr4_2:51530768-51530787 Msa0605590:CDS 40.0%
TCCAGGCACAAAGTTCTTTA+AGG - chr4_2:51530972-51530991 Msa0605590:CDS 40.0%
TCCTTAAAGAACTTTGTGCC+TGG + chr4_2:51530976-51530995 None:intergenic 40.0%
ATTGTTGGAAGAGTAGAGTC+CGG + chr4_2:51531015-51531034 None:intergenic 40.0%
CTCTACTCTTCCAACAATTG+AGG - chr4_2:51531017-51531036 Msa0605590:CDS 40.0%
CAATTGAGGTCTATATTGCC+GGG - chr4_2:51531031-51531050 Msa0605590:CDS 40.0%
AATAGTGTATCGCAGAACAG+TGG + chr4_2:51531372-51531391 None:intergenic 40.0%
GTTCTTAAATAGTGGCGCTA+TGG - chr4_2:51531403-51531422 Msa0605590:five_prime_UTR 40.0%
GCTATGGCATTGTACTTTAG+TGG - chr4_2:51531419-51531438 Msa0605590:five_prime_UTR 40.0%
! AGGTCTAGCGTTGCTTTAAT+TGG - chr4_2:51531487-51531506 Msa0605590:intron 40.0%
GGAGTCGTTTGAATATGACA+TGG - chr4_2:51531526-51531545 Msa0605590:intron 40.0%
TTTGAGAAATAGCTGTCCTC+CGG - chr4_2:51531700-51531719 Msa0605590:intron 40.0%
CGTATGCTGTTCTTGTGTAT+AGG + chr4_2:51531954-51531973 None:intergenic 40.0%
GAACTTTCATGGTTTAACGC+CGG - chr4_2:51531991-51532010 Msa0605590:intron 40.0%
AGAATAGCCGTCTTCTGAAT+TGG + chr4_2:51532110-51532129 None:intergenic 40.0%
AGATAAAGATGGCCGAACTA+AGG + chr4_2:51532193-51532212 None:intergenic 40.0%
TAGTTCGGCCATCTTTATCT+TGG - chr4_2:51532193-51532212 Msa0605590:five_prime_UTR 40.0%
TCTTTGGGCCAAGATAAAGA+TGG + chr4_2:51532204-51532223 None:intergenic 40.0%
TATTCCCCTACTTATTGAGG+TGG - chr4_2:51532368-51532387 Msa0605590:five_prime_UTR 40.0%
! AATCATGGTGGAGAGACTAA+TGG + chr4_2:51532447-51532466 None:intergenic 40.0%
GTGGTAAGAGAAGATGTGGA+TGG + chr4_2:51529542-51529561 None:intergenic 45.0%
TGAGGTGGTAAGAGAAGATG+TGG + chr4_2:51529546-51529565 None:intergenic 45.0%
!! GTGGATTAGTGTTGCTGTGT+TGG - chr4_2:51530405-51530424 Msa0605590:intron 45.0%
! GCTGTGTTGGTACAAGTCAA+TGG - chr4_2:51530418-51530437 Msa0605590:intron 45.0%
GGTAACGAACCCTTTATCGA+GGG + chr4_2:51530577-51530596 None:intergenic 45.0%
CGTTACCGTGGTTAGACATA+AGG - chr4_2:51530590-51530609 Msa0605590:intron 45.0%
AAGTGTGAGAATCTCAGGAG+TGG + chr4_2:51530656-51530675 None:intergenic 45.0%
GGATTCCAACCCTTCTCAAA+AGG - chr4_2:51530746-51530765 Msa0605590:CDS 45.0%
!! CTATCCCTTTTGAGAAGGGT+TGG + chr4_2:51530754-51530773 None:intergenic 45.0%
CAACCCTTCTCAAAAGGGAT+AGG - chr4_2:51530752-51530771 Msa0605590:CDS 45.0%
!! CACCCTATCCCTTTTGAGAA+GGG + chr4_2:51530758-51530777 None:intergenic 45.0%
! GAGTGGTAGTGTGAAGTTTG+AGG - chr4_2:51530828-51530847 Msa0605590:CDS 45.0%
! GGAGAAGCAATTGTTGTTGC+CGG - chr4_2:51530993-51531012 Msa0605590:CDS 45.0%
! TCTGGAATTGCATTCAACGC+CGG + chr4_2:51531129-51531148 None:intergenic 45.0%
GACTCCGTGTTGTCAAATTG+CGG - chr4_2:51531307-51531326 Msa0605590:CDS 45.0%
AGTGCCGCAATTTGACAACA+CGG + chr4_2:51531314-51531333 None:intergenic 45.0%
ACTATAGCGCTATGCCCTTT+AGG - chr4_2:51531331-51531350 Msa0605590:CDS 45.0%
CTATAGCGCTATGCCCTTTA+GGG - chr4_2:51531332-51531351 Msa0605590:CDS 45.0%
GACTGATGTCACAACCTAGT+AGG - chr4_2:51531575-51531594 Msa0605590:intron 45.0%
TGTCACAACCTAGTAGGAGA+TGG - chr4_2:51531581-51531600 Msa0605590:intron 45.0%
ACAAGAACAGCATACGCTGA+CGG - chr4_2:51531958-51531977 Msa0605590:intron 45.0%
! ATGGTTTAACGCCGGTGTTA+GGG - chr4_2:51531999-51532018 Msa0605590:intron 45.0%
!! GTTAGGGTTGGTGTTGGTAT+TGG - chr4_2:51532015-51532034 Msa0605590:intron 45.0%
GTTTGTCTTGGAATCGGCAT+TGG - chr4_2:51532045-51532064 Msa0605590:intron 45.0%
! AAATCATACCAAGGTGCCAC+CGG - chr4_2:51532084-51532103 Msa0605590:intron 45.0%
! GTCTTGTTCATCGAGTGCAA+AGG - chr4_2:51532236-51532255 Msa0605590:five_prime_UTR 45.0%
TTGCATGTGTCGTGTTTCGC+CGG - chr4_2:51529835-51529854 Msa0605590:CDS 50.0%
CGGTAACGAACCCTTTATCG+AGG + chr4_2:51530578-51530597 None:intergenic 50.0%
CGATAAAGGGTTCGTTACCG+TGG - chr4_2:51530578-51530597 Msa0605590:intron 50.0%
!! CCACCCTATCCCTTTTGAGA+AGG + chr4_2:51530759-51530778 None:intergenic 50.0%
CCTTCTCAAAAGGGATAGGG+TGG - chr4_2:51530756-51530775 Msa0605590:CDS 50.0%
TATATTGCCGGGTCCTTCTC+TGG - chr4_2:51531042-51531061 Msa0605590:CDS 50.0%
TGACAACACGGAGTCACACA+TGG + chr4_2:51531302-51531321 None:intergenic 50.0%
GCGAAGATGGGGAACTTTCA+TGG - chr4_2:51531980-51531999 Msa0605590:intron 50.0%
CATGGTTTAACGCCGGTGTT+AGG - chr4_2:51531998-51532017 Msa0605590:intron 50.0%
! TTTAACGCCGGTGTTAGGGT+TGG - chr4_2:51532003-51532022 Msa0605590:intron 50.0%
ACCAACACCAACCCTAACAC+CGG + chr4_2:51532013-51532032 None:intergenic 50.0%
! CTTGGAATCGGCATTGGAGT+TGG - chr4_2:51532051-51532070 Msa0605590:intron 50.0%
TAGCCGTCTTCTGAATTGGC+CGG + chr4_2:51532106-51532125 None:intergenic 50.0%
! GATGATCGGAGTGCCAGAGA+AGG + chr4_2:51531058-51531077 None:intergenic 55.0%
TCCTTCTGCGCTTGCTTTGC+AGG - chr4_2:51531176-51531195 Msa0605590:CDS 55.0%
ACCTGCAAAGCAAGCGCAGA+AGG + chr4_2:51531180-51531199 None:intergenic 55.0%
CATACGCTGACGGCGAAGAT+GGG - chr4_2:51531968-51531987 Msa0605590:intron 55.0%
ATACGCTGACGGCGAAGATG+GGG - chr4_2:51531969-51531988 Msa0605590:intron 55.0%
CCACCGGCCAATTCAGAAGA+CGG - chr4_2:51532100-51532119 Msa0605590:intron 55.0%
GCATACGCTGACGGCGAAGA+TGG - chr4_2:51531967-51531986 Msa0605590:intron 60.0%
!! GCCGGTGTTAGGGTTGGTGT+TGG - chr4_2:51532009-51532028 Msa0605590:intron 60.0%
TGAATTGGCCGGTGGCACCT+TGG + chr4_2:51532095-51532114 None:intergenic 60.0%
CCGTCTTCTGAATTGGCCGG+TGG + chr4_2:51532103-51532122 None:intergenic 60.0%
! CGGAGTGCCAGAGAAGGACC+CGG + chr4_2:51531052-51531071 None:intergenic 65.0%
TGCATGTGCTCTCGAGCCGG+AGG + chr4_2:51531719-51531738 None:intergenic 65.0%
! TCCGGCTCGAGAGCACATGC+AGG - chr4_2:51531718-51531737 Msa0605590:intron 65.0%
GCCTGCATGTGCTCTCGAGC+CGG + chr4_2:51531722-51531741 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51529330 51532545 51529330 ID=Msa0605590;Name=Msa0605590
chr4_2 mRNA 51529330 51532545 51529330 ID=Msa0605590-mRNA-1;Parent=Msa0605590;Name=Msa0605590-mRNA-1;_AED=0.17;_eAED=0.17;_QI=498|1|1|1|0.5|0.33|3|414|310
chr4_2 exon 51529330 51529971 51529330 ID=Msa0605590-mRNA-1:exon:12383;Parent=Msa0605590-mRNA-1
chr4_2 exon 51530678 51531469 51530678 ID=Msa0605590-mRNA-1:exon:12382;Parent=Msa0605590-mRNA-1
chr4_2 exon 51532135 51532545 51532135 ID=Msa0605590-mRNA-1:exon:12381;Parent=Msa0605590-mRNA-1
chr4_2 five_prime_UTR 51532135 51532545 51532135 ID=Msa0605590-mRNA-1:five_prime_utr;Parent=Msa0605590-mRNA-1
chr4_2 five_prime_UTR 51531383 51531469 51531383 ID=Msa0605590-mRNA-1:five_prime_utr;Parent=Msa0605590-mRNA-1
chr4_2 CDS 51530678 51531382 51530678 ID=Msa0605590-mRNA-1:cds;Parent=Msa0605590-mRNA-1
chr4_2 CDS 51529744 51529971 51529744 ID=Msa0605590-mRNA-1:cds;Parent=Msa0605590-mRNA-1
chr4_2 three_prime_UTR 51529330 51529743 51529330 ID=Msa0605590-mRNA-1:three_prime_utr;Parent=Msa0605590-mRNA-1
Gene Sequence

>Msa0605590

ATGGAGAATTCGTATAAGGTTAATGGAAATGGACCAAGTGAAAATGGATACTCTATTGCTAGGCACACACACTCTTATCAACCCTCGATAAAGGGTTCGTTACCGTGGTTAGACATAAGGGTGTTTTATGTAAGGGTTTGCAAGTGTGAGCTTGATAATTCCACTCCTGAGATTCTCACACTTAATCATGTTCCTTTAAATCCTGATACCCTTCTTGAAGTTAATGGTGTTCGGAGTAGTTTATATTCTGATGGGATTCCAACCCTTCTCAAAAGGGATAGGGTGGATAGAAAATCGGAAGAAGTTACGTTTGTAAGCACTGATAGTATAAGGATGAGTGGTAGTGTGAAGTTTGAGGTGTTTGATAAAGATAAGCTTCTTCTGTTTGGTACCTTAGAATTATGTAACAGCAATGGTGTTGTTAGAGAATCAAATTCTAATGGACAAAGTTGGAGCATGAAGTGTGAGTCGAATATAATTCCAGGCACAAAGTTCTTTAAGGAGAAGCAATTGTTGTTGCCGGACTCTACTCTTCCAACAATTGAGGTCTATATTGCCGGGTCCTTCTCTGGCACTCCGATCATCTTAACGAAGACCTTGCATCTTAGTTCTCAAAAAAGACATACAAGGAAGCCGGCGTTGAATGCAATTCCAGAGAATGATGCAAATGAAAATGGGAAAGGTCCTTCTGCGCTTGCTTTGCAGGCTCCAGATTACATGTATGACAAACATGATGATGAGGATTATCACGGCCTATACACAAGAACAGCATACGCTGACGGCGAAGATGGGGAACTTTCATGGTTTAACGCCGGTGTTAGGGTTGGTGTTGGTATTGGACTTAGTGTTTGTCTTGGAATCGGCATTGGAGTTGGCATACTCGTTAAATCATACCAAGGTGCCACCGGCCAATTCAGAAGACGGCTATTCTAA
Protein sequence

>Msa0605590

MENSYKVNGNGPSENGYSIARHTHSYQPSIKGSLPWLDIRVFYVRVCKCELDNSTPEILTLNHVPLNPDTLLEVNGVRSSLYSDGIPTLLKRDRVDRKSEEVTFVSTDSIRMSGSVKFEVFDKDKLLLFGTLELCNSNGVVRESNSNGQSWSMKCESNIIPGTKFFKEKQLLLPDSTLPTIEVYIAGSFSGTPIILTKTLHLSSQKRHTRKPALNAIPENDANENGKGPSALALQAPDYMYDKHDDEDYHGLYTRTAYADGEDGELSWFNAGVRVGVGIGLSVCLGIGIGVGILVKSYQGATGQFRRRLF*