AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0646540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646540 MtrunA17_Chr4g0034951 98.392 311 4 1 1 310 1 311 0.0 629
Msa0646540 MtrunA17_Chr1g0155081 49.320 294 127 7 26 309 30 311 5.33e-91 273
Msa0646540 MtrunA17_Chr8g0348371 41.853 313 154 9 14 310 3 303 3.68e-66 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646540 AT1G19400.1 54.485 301 127 5 13 310 10 303 1.17e-107 316
Msa0646540 AT1G19400.3 54.485 301 127 5 13 310 10 303 1.17e-107 316
Msa0646540 AT1G19400.2 54.485 301 127 5 13 310 10 303 1.17e-107 316
Msa0646540 AT1G75180.1 56.934 274 114 3 41 310 42 315 5.72e-107 314
Msa0646540 AT1G75180.3 56.934 274 114 3 41 310 42 315 5.72e-107 314
Msa0646540 AT1G19400.4 54.185 227 98 3 13 236 10 233 7.81e-79 240
Msa0646540 AT1G75180.2 56.477 193 82 1 41 231 42 234 1.37e-72 224
Msa0646540 AT1G75180.4 56.477 193 82 1 41 231 42 234 1.53e-71 223
Msa0646540 AT1G01500.1 41.892 296 141 10 36 310 42 327 1.13e-63 204

Find 131 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGGCACAAAGTTCTTTA+AGG 0.155540 4_3:-52323629 Msa0646540:CDS
AGGTGGTAATAAATGATTTA+AGG 0.188726 4_3:-52322216 Msa0646540:three_prime_UTR
ATGGATATTGTTTAATTTAA+TGG 0.209398 4_3:+52325057 None:intergenic
GATACCCTTCTTGAAGTTAA+TGG 0.212761 4_3:-52323904 Msa0646540:CDS
GAGAATGATGCAAATGAAAA+TGG 0.217056 4_3:-52323454 Msa0646540:CDS
TCCTTCTGCGCTTGCTTTGC+AGG 0.233001 4_3:-52323425 Msa0646540:intron
CTTTAAGCTTCAATAGATTC+TGG 0.234279 4_3:-52324957 Msa0646540:five_prime_UTR
AGACATTAACATGTTGTCTT+TGG 0.250262 4_3:+52322384 None:intergenic
CATGGTTTAACGCCGGTGTT+AGG 0.255714 4_3:-52322603 Msa0646540:CDS
AAGAACAAATCTTGCAATCT+TGG 0.266859 4_3:+52324977 None:intergenic
GAGAATTCGTATAAGGTTAA+TGG 0.283457 4_3:-52324105 Msa0646540:CDS
GGAAATGGACCAAGTGAAAA+TGG 0.283915 4_3:-52324084 Msa0646540:CDS
GTTAGGGTTGGTGTTGGTAT+TGG 0.297925 4_3:-52322586 Msa0646540:CDS
ATTGCTGTTACATAATTCTA+AGG 0.316159 4_3:+52323717 None:intergenic
GGATATATATTATATAGTTT+TGG 0.316791 4_3:-52322344 Msa0646540:three_prime_UTR
CTCATATCAACCCTCGATAA+AGG 0.321091 4_3:-52324037 Msa0646540:CDS
AGAAGGGTATCAGGATTTAA+AGG 0.331368 4_3:+52323916 None:intergenic
GGAGAAGCAATTGTTGTTGC+CGG 0.344534 4_3:-52323608 Msa0646540:CDS
GACATTAACATGTTGTCTTT+GGG 0.346532 4_3:+52322385 None:intergenic
AAAGATAAGCTTCTTCTGTT+TGG 0.350138 4_3:-52323742 Msa0646540:CDS
TTAACTTCAAGAAGGGTATC+AGG 0.353127 4_3:+52323907 None:intergenic
ATCAAAACATAGGTTGTTAT+CGG 0.360179 4_3:+52325005 None:intergenic
TCGTATAAGGTTAATGGAAA+TGG 0.363071 4_3:-52324099 Msa0646540:CDS
GGATCGATTACTTGCAAGAT+TGG 0.371568 4_3:+52325255 None:intergenic
GCGAAGATGGGGAACTTTCA+TGG 0.371984 4_3:-52322621 Msa0646540:CDS
AGAATAGCCGTCTTCTGAAT+TGG 0.372025 4_3:+52322494 None:intergenic
AGAACAAATCTTGCAATCTT+GGG 0.374948 4_3:+52324978 None:intergenic
GTTAGAGAATCAAATTCTAA+TGG 0.378979 4_3:-52323688 Msa0646540:CDS
TATATTGCCGGGTCCTTCTC+TGG 0.379377 4_3:-52323559 Msa0646540:CDS
TAGTTCGGCCATCTTTATCT+TGG 0.380101 4_3:-52322408 Msa0646540:three_prime_UTR
CGTTACCGTGGTTAGACATA+AGG 0.381604 4_3:-52324011 Msa0646540:CDS
TGATCAACATTTATAAAGTA+AGG 0.389780 4_3:-52322084 Msa0646540:three_prime_UTR
ACAATTGAGGTCTATATTGC+CGG 0.393367 4_3:-52323571 Msa0646540:CDS
TTGTAAGCACTGATAGTATA+AGG 0.394406 4_3:-52323798 Msa0646540:CDS
TTTAAGCTTCAATAGATTCT+GGG 0.398434 4_3:-52324956 Msa0646540:five_prime_UTR
TGTTTGTCATACATGTAATC+TGG 0.402010 4_3:+52322694 None:intergenic
GAAAACTGAAATGTTATGTT+GGG 0.402948 4_3:+52325210 None:intergenic
GGTGGTAATAAATGATTTAA+GGG 0.404373 4_3:-52322215 Msa0646540:three_prime_UTR
CGCGATGGAGAATTCGTATA+AGG 0.406902 4_3:-52324112 Msa0646540:exon
TACTTTATAAATGTTGATCA+TGG 0.417685 4_3:+52322087 None:intergenic
TTTGTGTGTGAATTAATTAG+AGG 0.422085 4_3:+52325152 None:intergenic
TTCTTGAAGTTAATGGTGTT+CGG 0.422504 4_3:-52323897 Msa0646540:CDS
CTTAGTGTTTGTCTTGGAAT+CGG 0.423405 4_3:-52322562 Msa0646540:CDS
TGATTAAGTGTGAGAATCTC+AGG 0.428692 4_3:+52323943 None:intergenic
CGGAGTAGTTTATATTCTGA+TGG 0.435949 4_3:-52323877 Msa0646540:CDS
GCAATATAGACCTCAATTGT+TGG 0.437528 4_3:+52323574 None:intergenic
TTATATTCCCCTACTTATTG+AGG 0.438498 4_3:-52322236 Msa0646540:three_prime_UTR
CTTGGAATCGGCATTGGAGT+TGG 0.441010 4_3:-52322550 Msa0646540:CDS
TTATTACCACCTCAATAAGT+AGG 0.441077 4_3:+52322227 None:intergenic
GGATTCCAACCCTTCTCAAA+AGG 0.443968 4_3:-52323855 Msa0646540:CDS
TCCTTAAAGAACTTTGTGCC+TGG 0.447833 4_3:+52323628 None:intergenic
GGATAGGGTGGATAGAAAAT+CGG 0.450168 4_3:-52323833 Msa0646540:CDS
GTTTGTCTTGGAATCGGCAT+TGG 0.451016 4_3:-52322556 Msa0646540:CDS
TGTGAGTCGAATATAATTCC+AGG 0.452189 4_3:-52323646 Msa0646540:CDS
TGAAAACTGAAATGTTATGT+TGG 0.452656 4_3:+52325209 None:intergenic
AAGAATAATATCAGATTCAA+AGG 0.458806 4_3:-52325092 Msa0646540:five_prime_UTR
ATGGTTTAACGCCGGTGTTA+GGG 0.458989 4_3:-52322602 Msa0646540:CDS
GAATTAATTAGAGGAAGAAA+AGG 0.460023 4_3:+52325161 None:intergenic
TCATATCAACCCTCGATAAA+GGG 0.470195 4_3:-52324036 Msa0646540:CDS
AGAATGATGCAAATGAAAAT+GGG 0.470852 4_3:-52323453 Msa0646540:CDS
CGTATGCTGTTCTTGTGTAT+AGG 0.473133 4_3:+52322650 None:intergenic
TGAATTGGCCGGTGGCACCT+TGG 0.475731 4_3:+52322509 None:intergenic
CAACCCTTCTCAAAAGGGAT+AGG 0.480345 4_3:-52323849 Msa0646540:CDS
ATTGGACTTAGTGTTTGTCT+TGG 0.481547 4_3:-52322568 Msa0646540:CDS
TATTACCACCTCAATAAGTA+GGG 0.486522 4_3:+52322228 None:intergenic
GCATACGCTGACGGCGAAGA+TGG 0.492307 4_3:-52322634 Msa0646540:CDS
CAAATTCTAATGGACAAAGT+TGG 0.492533 4_3:-52323678 Msa0646540:CDS
AAGGAGAGTGTGAAAATTGT+TGG 0.497096 4_3:+52325180 None:intergenic
AGATAAAGATGGCCGAACTA+AGG 0.499057 4_3:+52322411 None:intergenic
GTTACCGTGGTTAGACATAA+GGG 0.505953 4_3:-52324010 Msa0646540:CDS
CATACGCTGACGGCGAAGAT+GGG 0.511131 4_3:-52322633 Msa0646540:CDS
GATTCCAACCCTTCTCAAAA+GGG 0.526688 4_3:-52323854 Msa0646540:CDS
GGAGTAGTTTATATTCTGAT+GGG 0.528712 4_3:-52323876 Msa0646540:CDS
GATCAACATTTATAAAGTAA+GGG 0.530296 4_3:-52322083 Msa0646540:three_prime_UTR
ACCTGCAAAGCAAGCGCAGA+AGG 0.534939 4_3:+52323424 None:intergenic
AGAATAATATCAGATTCAAA+GGG 0.538270 4_3:-52325091 Msa0646540:five_prime_UTR
GGTTGTTATCGGTTATGTTG+AGG 0.539393 4_3:+52325016 None:intergenic
CAAAGAAACTGAGAAAATCA+TGG 0.543052 4_3:+52322142 None:intergenic
AGTTAACTTTGTTGTTGCAG+TGG 0.548009 4_3:-52324191 Msa0646540:five_prime_UTR
AACCCTTCTCAAAAGGGATA+GGG 0.548703 4_3:-52323848 Msa0646540:CDS
GAGTGGTAGTGTGAAGTTTG+AGG 0.549063 4_3:-52323773 Msa0646540:CDS
GCCGGTGTTAGGGTTGGTGT+TGG 0.549235 4_3:-52322592 Msa0646540:CDS
AATCATGGTGGAGAGACTAA+TGG 0.550185 4_3:+52322157 None:intergenic
CTTGGGATGAATCAAAACAT+AGG 0.551006 4_3:+52324995 None:intergenic
GTTTAATTTAATGGAAGATG+CGG 0.551396 4_3:+52325066 None:intergenic
CTCTACTCTTCCAACAATTG+AGG 0.556408 4_3:-52323584 Msa0646540:CDS
TATTCCCCTACTTATTGAGG+TGG 0.561142 4_3:-52322233 Msa0646540:three_prime_UTR
TTTAATTTAATGGAAGATGC+GGG 0.562035 4_3:+52325067 None:intergenic
TGAGGTGGTAAGAGAAGATG+TGG 0.564391 4_3:+52325034 None:intergenic
GTGGTAATAAATGATTTAAG+GGG 0.565067 4_3:-52322214 Msa0646540:three_prime_UTR
AAATCATACCAAGGTGCCAC+CGG 0.568352 4_3:-52322517 Msa0646540:CDS
GAACACCATTAACTTCAAGA+AGG 0.569037 4_3:+52323899 None:intergenic
TTTAACGCCGGTGTTAGGGT+TGG 0.570434 4_3:-52322598 Msa0646540:CDS
TGTTATCGGTTATGTTGAGG+TGG 0.575774 4_3:+52325019 None:intergenic
TAGCCGTCTTCTGAATTGGC+CGG 0.581948 4_3:+52322498 None:intergenic
CAATTGAGGTCTATATTGCC+GGG 0.582339 4_3:-52323570 Msa0646540:CDS
ATCAACATTTATAAAGTAAG+GGG 0.586146 4_3:-52322082 Msa0646540:three_prime_UTR
CCACCGGCCAATTCAGAAGA+CGG 0.588469 4_3:-52322501 Msa0646540:CDS
TCTGGAATTGCATTCAACGC+CGG 0.588676 4_3:+52323475 None:intergenic
AAAATGGATACTCTATTGCT+AGG 0.589815 4_3:-52324068 Msa0646540:CDS
CGATAAAGGGTTCGTTACCG+TGG 0.592561 4_3:-52324023 Msa0646540:CDS
AAGGTCTTCGTTAAGATGAT+CGG 0.592566 4_3:+52323532 None:intergenic
CGGAGTGCCAGAGAAGGACC+CGG 0.592882 4_3:+52323552 None:intergenic
GATGCAAATGAAAATGGGAA+AGG 0.596141 4_3:-52323448 Msa0646540:CDS
AACACCATTAACTTCAAGAA+GGG 0.596194 4_3:+52323900 None:intergenic
CCTTCTCAAAAGGGATAGGG+TGG 0.600087 4_3:-52323845 Msa0646540:CDS
ATTGTTGGAAGAGTAGAGTC+CGG 0.603903 4_3:+52323589 None:intergenic
TGGTAATAAATGATTTAAGG+GGG 0.603940 4_3:-52322213 Msa0646540:three_prime_UTR
GTGGTAAGAGAAGATGTGGA+TGG 0.604928 4_3:+52325038 None:intergenic
ACAAGAACAGCATACGCTGA+CGG 0.606360 4_3:-52322643 Msa0646540:CDS
TCTTTGGGCCAAGATAAAGA+TGG 0.610464 4_3:+52322400 None:intergenic
AAAGTAGAACTGTCTCGCGA+TGG 0.611108 4_3:-52324127 Msa0646540:exon
ACCAACACCAACCCTAACAC+CGG 0.618909 4_3:+52322591 None:intergenic
AAAACTGAAATGTTATGTTG+GGG 0.627111 4_3:+52325211 None:intergenic
CGGTAACGAACCCTTTATCG+AGG 0.628590 4_3:+52324026 None:intergenic
ATACGCTGACGGCGAAGATG+GGG 0.629010 4_3:-52322632 Msa0646540:CDS
ACTGATAGTATAAGGATGAG+TGG 0.634943 4_3:-52323790 Msa0646540:CDS
CATGATGATGAGGATTATCA+CGG 0.635768 4_3:-52322673 Msa0646540:CDS
GATGATCGGAGTGCCAGAGA+AGG 0.637274 4_3:+52323546 None:intergenic
CCGTCTTCTGAATTGGCCGG+TGG 0.642624 4_3:+52322501 None:intergenic
GGTAACGAACCCTTTATCGA+GGG 0.642976 4_3:+52324027 None:intergenic
AACACCCTTATGTCTAACCA+CGG 0.647619 4_3:+52324006 None:intergenic
TTAGAATTATGTAACAGCAA+TGG 0.648523 4_3:-52323715 Msa0646540:CDS
GAACTTTCATGGTTTAACGC+CGG 0.650464 4_3:-52322610 Msa0646540:CDS
ATTACCACCTCAATAAGTAG+GGG 0.652127 4_3:+52322229 None:intergenic
AAGTGTGAGAATCTCAGGAG+TGG 0.662685 4_3:+52323948 None:intergenic
GTATGACAAACATGATGATG+AGG 0.669080 4_3:-52322683 Msa0646540:CDS
GTCTTGTTCATCGAGTGCAA+AGG 0.669479 4_3:-52322365 Msa0646540:three_prime_UTR
TGATTCTTACAGTAATCACA+AGG 0.698127 4_3:-52322182 Msa0646540:three_prime_UTR
ATACTCGTTAAATCATACCA+AGG 0.711189 4_3:-52322526 Msa0646540:CDS
AGAAACTGAGAAAATCATGG+TGG 0.715746 4_3:+52322145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAATTTGATTTTTAATTT+TGG - chr4_3:52322465-52322484 Msa0646540:three_prime_UTR 10.0%
!!! ATGGATATTGTTTAATTTAA+TGG + chr4_3:52322286-52322305 None:intergenic 15.0%
!!! AAATTTTGATTATTGTTATG+TGG + chr4_3:52322453-52322472 None:intergenic 15.0%
!! CAAAGAAATATAAGAATTAA+AGG + chr4_3:52322850-52322869 None:intergenic 15.0%
!! TTTACAAAAGAAAATCAAAA+AGG + chr4_3:52323117-52323136 None:intergenic 15.0%
!! AATATAACTGTAAATTATCT+TGG - chr4_3:52324359-52324378 Msa0646540:intron 15.0%
!!! AAATTTTTAAAATCTTGTCA+AGG + chr4_3:52324529-52324548 None:intergenic 15.0%
!! AGCATTTACATAAAATAATA+AGG + chr4_3:52324619-52324638 None:intergenic 15.0%
!!! GGATATATATTATATAGTTT+TGG - chr4_3:52324996-52325015 Msa0646540:five_prime_UTR 15.0%
!! AAGAATAATATCAGATTCAA+AGG - chr4_3:52322248-52322267 Msa0646540:three_prime_UTR 20.0%
!! AGAATAATATCAGATTCAAA+GGG - chr4_3:52322249-52322268 Msa0646540:three_prime_UTR 20.0%
!!! TTGAATTTTGCATTAGTTAA+TGG - chr4_3:52322533-52322552 Msa0646540:CDS 20.0%
!!! TGAATTTTGTTTTGCTTTAT+TGG - chr4_3:52322886-52322905 Msa0646540:intron 20.0%
!! CAATCAGATAAAAATAATAC+AGG + chr4_3:52322919-52322938 None:intergenic 20.0%
!!! TATTTTTATCTGATTGAGTA+AGG - chr4_3:52322923-52322942 Msa0646540:intron 20.0%
!!! ATTTATTTGTGCTTTTATGT+TGG - chr4_3:52323048-52323067 Msa0646540:intron 20.0%
!! ATTTAAATAAGCACATGAAA+AGG + chr4_3:52323964-52323983 None:intergenic 20.0%
!!! GGAATTATCTATAATTTGTT+GGG + chr4_3:52324598-52324617 None:intergenic 20.0%
!!! AGGAATTATCTATAATTTGT+TGG + chr4_3:52324599-52324618 None:intergenic 20.0%
!! TACTTTATAAATGTTGATCA+TGG + chr4_3:52325256-52325275 None:intergenic 20.0%
!! TGATCAACATTTATAAAGTA+AGG - chr4_3:52325256-52325275 Msa0646540:five_prime_UTR 20.0%
!! GATCAACATTTATAAAGTAA+GGG - chr4_3:52325257-52325276 Msa0646540:five_prime_UTR 20.0%
!! ATCAACATTTATAAAGTAAG+GGG - chr4_3:52325258-52325277 Msa0646540:five_prime_UTR 20.0%
! AAAACTGAAATGTTATGTTG+GGG + chr4_3:52322132-52322151 None:intergenic 25.0%
! GAAAACTGAAATGTTATGTT+GGG + chr4_3:52322133-52322152 None:intergenic 25.0%
! TGAAAACTGAAATGTTATGT+TGG + chr4_3:52322134-52322153 None:intergenic 25.0%
! GAATTAATTAGAGGAAGAAA+AGG + chr4_3:52322182-52322201 None:intergenic 25.0%
! TTTGTGTGTGAATTAATTAG+AGG + chr4_3:52322191-52322210 None:intergenic 25.0%
!! TTTAATTTAATGGAAGATGC+GGG + chr4_3:52322276-52322295 None:intergenic 25.0%
!! GTTTAATTTAATGGAAGATG+CGG + chr4_3:52322277-52322296 None:intergenic 25.0%
! ATCAAAACATAGGTTGTTAT+CGG + chr4_3:52322338-52322357 None:intergenic 25.0%
! TTTAAGCTTCAATAGATTCT+GGG - chr4_3:52322384-52322403 Msa0646540:three_prime_UTR 25.0%
! ATTTAGACAAAAAAAACAGC+CGG + chr4_3:52322620-52322639 None:intergenic 25.0%
! GAAAATCAAAAAGGTTGAAA+AGG + chr4_3:52323108-52323127 None:intergenic 25.0%
!! ATTGCTGTTACATAATTCTA+AGG + chr4_3:52323626-52323645 None:intergenic 25.0%
! TTAGAATTATGTAACAGCAA+TGG - chr4_3:52323625-52323644 Msa0646540:CDS 25.0%
! GTTAGAGAATCAAATTCTAA+TGG - chr4_3:52323652-52323671 Msa0646540:CDS 25.0%
! AGAATGATGCAAATGAAAAT+GGG - chr4_3:52323887-52323906 Msa0646540:CDS 25.0%
!! TACAAAGTGTTCTTAAATAG+TGG - chr4_3:52324134-52324153 Msa0646540:five_prime_UTR 25.0%
! ATATTTATGACCAAACCAAA+TGG - chr4_3:52324549-52324568 Msa0646540:intron 25.0%
! TTCTTCATAAGATTTCCATT+TGG + chr4_3:52324567-52324586 None:intergenic 25.0%
!!! TCTATAATTTGTTGGGAAAA+TGG + chr4_3:52324591-52324610 None:intergenic 25.0%
!!! TTATTTTATGTAAATGCTGC+AGG - chr4_3:52324621-52324640 Msa0646540:intron 25.0%
! AGGTGGTAATAAATGATTTA+AGG - chr4_3:52325124-52325143 Msa0646540:five_prime_UTR 25.0%
! GGTGGTAATAAATGATTTAA+GGG - chr4_3:52325125-52325144 Msa0646540:five_prime_UTR 25.0%
! GTGGTAATAAATGATTTAAG+GGG - chr4_3:52325126-52325145 Msa0646540:five_prime_UTR 25.0%
! TGGTAATAAATGATTTAAGG+GGG - chr4_3:52325127-52325146 Msa0646540:five_prime_UTR 25.0%
!!! AACATTTTTTGTCTGAGAAA+AGG + chr4_3:52325224-52325243 None:intergenic 25.0%
!!! TTTTCTGTTTGCTGTATAAG+GGG + chr4_3:52322109-52322128 None:intergenic 30.0%
!!! GTTTTCTGTTTGCTGTATAA+GGG + chr4_3:52322110-52322129 None:intergenic 30.0%
AGAACAAATCTTGCAATCTT+GGG + chr4_3:52322365-52322384 None:intergenic 30.0%
AAGAACAAATCTTGCAATCT+TGG + chr4_3:52322366-52322385 None:intergenic 30.0%
CTTTAAGCTTCAATAGATTC+TGG - chr4_3:52322383-52322402 Msa0646540:three_prime_UTR 30.0%
! TTTTGGCACAAGATATTTTG+TGG - chr4_3:52322482-52322501 Msa0646540:exon 30.0%
ACCTGAAAAACAGACAAAAA+GGG + chr4_3:52322776-52322795 None:intergenic 30.0%
! TATTTGTGCTTTTATGTTGG+TGG - chr4_3:52323051-52323070 Msa0646540:intron 30.0%
GAGAATTCGTATAAGGTTAA+TGG - chr4_3:52323235-52323254 Msa0646540:intron 30.0%
TCGTATAAGGTTAATGGAAA+TGG - chr4_3:52323241-52323260 Msa0646540:intron 30.0%
!! ATAGAGTATCCATTTTCACT+TGG + chr4_3:52323268-52323287 None:intergenic 30.0%
AAAATGGATACTCTATTGCT+AGG - chr4_3:52323272-52323291 Msa0646540:intron 30.0%
!!! ACATAAGGGTGTTTTATGTA+AGG - chr4_3:52323344-52323363 Msa0646540:intron 30.0%
!!! CATAAGGGTGTTTTATGTAA+GGG - chr4_3:52323345-52323364 Msa0646540:intron 30.0%
AACACCATTAACTTCAAGAA+GGG + chr4_3:52323443-52323462 None:intergenic 30.0%
TTCTTGAAGTTAATGGTGTT+CGG - chr4_3:52323443-52323462 Msa0646540:CDS 30.0%
GGAGTAGTTTATATTCTGAT+GGG - chr4_3:52323464-52323483 Msa0646540:CDS 30.0%
!! TTGTAAGCACTGATAGTATA+AGG - chr4_3:52323542-52323561 Msa0646540:CDS 30.0%
AAAGATAAGCTTCTTCTGTT+TGG - chr4_3:52323598-52323617 Msa0646540:CDS 30.0%
! CAAATTCTAATGGACAAAGT+TGG - chr4_3:52323662-52323681 Msa0646540:CDS 30.0%
!! TTTTTGAGAACTAAGATGCA+AGG + chr4_3:52323830-52323849 None:intergenic 30.0%
GTTCTCAAAAAAGACATACA+AGG - chr4_3:52323839-52323858 Msa0646540:CDS 30.0%
! TTTTCATTTGCATCATTCTC+TGG + chr4_3:52323886-52323905 None:intergenic 30.0%
GAGAATGATGCAAATGAAAA+TGG - chr4_3:52323886-52323905 Msa0646540:CDS 30.0%
ATTCAAGCAACAAGTTAACA+AGG + chr4_3:52324016-52324035 None:intergenic 30.0%
! GATTTGTTCAAATCCCTAAA+GGG + chr4_3:52324087-52324106 None:intergenic 30.0%
TAATATGCGATTGACAATAC+AGG - chr4_3:52324206-52324225 Msa0646540:intron 30.0%
!! ATTGGCTCAACGTAATTTTA+TGG - chr4_3:52324244-52324263 Msa0646540:intron 30.0%
CATAAGATTTCCATTTGGTT+TGG + chr4_3:52324562-52324581 None:intergenic 30.0%
TGTTTGTCATACATGTAATC+TGG + chr4_3:52324649-52324668 None:intergenic 30.0%
ATACTCGTTAAATCATACCA+AGG - chr4_3:52324814-52324833 Msa0646540:intron 30.0%
AAAAACAGCTAACCTTAGTT+CGG - chr4_3:52324917-52324936 Msa0646540:five_prime_UTR 30.0%
GACATTAACATGTTGTCTTT+GGG + chr4_3:52324958-52324977 None:intergenic 30.0%
AGACATTAACATGTTGTCTT+TGG + chr4_3:52324959-52324978 None:intergenic 30.0%
TTATATTCCCCTACTTATTG+AGG - chr4_3:52325104-52325123 Msa0646540:five_prime_UTR 30.0%
TATTACCACCTCAATAAGTA+GGG + chr4_3:52325115-52325134 None:intergenic 30.0%
TTATTACCACCTCAATAAGT+AGG + chr4_3:52325116-52325135 None:intergenic 30.0%
TGATTCTTACAGTAATCACA+AGG - chr4_3:52325158-52325177 Msa0646540:five_prime_UTR 30.0%
CAAAGAAACTGAGAAAATCA+TGG + chr4_3:52325201-52325220 None:intergenic 30.0%
!!! GGTTTTCTGTTTGCTGTATA+AGG + chr4_3:52322111-52322130 None:intergenic 35.0%
! AAGGAGAGTGTGAAAATTGT+TGG + chr4_3:52322163-52322182 None:intergenic 35.0%
CTTGGGATGAATCAAAACAT+AGG + chr4_3:52322348-52322367 None:intergenic 35.0%
! GTTAATGGATGTGTTAGTGA+CGG - chr4_3:52322548-52322567 Msa0646540:CDS 35.0%
CCTCAAACATGTCAACTATT+CGG + chr4_3:52322732-52322751 None:intergenic 35.0%
CCTGAAAAACAGACAAAAAG+GGG + chr4_3:52322775-52322794 None:intergenic 35.0%
GACCTGAAAAACAGACAAAA+AGG + chr4_3:52322777-52322796 None:intergenic 35.0%
!!! TTTTGTCTGTTTTTCAGGTC+TGG - chr4_3:52322777-52322796 Msa0646540:intron 35.0%
!! AGTTAACTTTGTTGTTGCAG+TGG - chr4_3:52323149-52323168 Msa0646540:intron 35.0%
TCATATCAACCCTCGATAAA+GGG - chr4_3:52323304-52323323 Msa0646540:intron 35.0%
TGATTAAGTGTGAGAATCTC+AGG + chr4_3:52323400-52323419 None:intergenic 35.0%
!! AGAAGGGTATCAGGATTTAA+AGG + chr4_3:52323427-52323446 None:intergenic 35.0%
!! TTAACTTCAAGAAGGGTATC+AGG + chr4_3:52323436-52323455 None:intergenic 35.0%
GATACCCTTCTTGAAGTTAA+TGG - chr4_3:52323436-52323455 Msa0646540:CDS 35.0%
GAACACCATTAACTTCAAGA+AGG + chr4_3:52323444-52323463 None:intergenic 35.0%
CGGAGTAGTTTATATTCTGA+TGG - chr4_3:52323463-52323482 Msa0646540:CDS 35.0%
ACTGATAGTATAAGGATGAG+TGG - chr4_3:52323550-52323569 Msa0646540:CDS 35.0%
TGTGAGTCGAATATAATTCC+AGG - chr4_3:52323694-52323713 Msa0646540:CDS 35.0%
GCAATATAGACCTCAATTGT+TGG + chr4_3:52323769-52323788 None:intergenic 35.0%
ACAATTGAGGTCTATATTGC+CGG - chr4_3:52323769-52323788 Msa0646540:CDS 35.0%
AAGGTCTTCGTTAAGATGAT+CGG + chr4_3:52323811-52323830 None:intergenic 35.0%
AAAAAGACATACAAGGAAGC+CGG - chr4_3:52323846-52323865 Msa0646540:CDS 35.0%
GATGCAAATGAAAATGGGAA+AGG - chr4_3:52323892-52323911 Msa0646540:CDS 35.0%
! ATGCACTGACATAGTTAAAG+AGG + chr4_3:52323990-52324009 None:intergenic 35.0%
! CGATTTGTTCAAATCCCTAA+AGG + chr4_3:52324088-52324107 None:intergenic 35.0%
ATTATGTTCCATCTCCTACT+AGG + chr4_3:52324331-52324350 None:intergenic 35.0%
!! TTATCTTGGCTCTTTTTCTG+AGG - chr4_3:52324373-52324392 Msa0646540:intron 35.0%
! AACTATTTTCATCACTCGTC+AGG + chr4_3:52324505-52324524 None:intergenic 35.0%
! GTATGACAAACATGATGATG+AGG - chr4_3:52324657-52324676 Msa0646540:intron 35.0%
! CATGATGATGAGGATTATCA+CGG - chr4_3:52324667-52324686 Msa0646540:intron 35.0%
ATTGGACTTAGTGTTTGTCT+TGG - chr4_3:52324772-52324791 Msa0646540:intron 35.0%
CTTAGTGTTTGTCTTGGAAT+CGG - chr4_3:52324778-52324797 Msa0646540:intron 35.0%
!!! TAGCTGTTTTTGGATCAAGA+GGG + chr4_3:52324908-52324927 None:intergenic 35.0%
!!! TTAGCTGTTTTTGGATCAAG+AGG + chr4_3:52324909-52324928 None:intergenic 35.0%
!! GAACTAAGGTTAGCTGTTTT+TGG + chr4_3:52324918-52324937 None:intergenic 35.0%
ATTACCACCTCAATAAGTAG+GGG + chr4_3:52325114-52325133 None:intergenic 35.0%
ATAAATGATTTAAGGGGGCT+TGG - chr4_3:52325132-52325151 Msa0646540:five_prime_UTR 35.0%
AGAAACTGAGAAAATCATGG+TGG + chr4_3:52325198-52325217 None:intergenic 35.0%
GGATCGATTACTTGCAAGAT+TGG + chr4_3:52322088-52322107 None:intergenic 40.0%
! TCCTCTCTTTTTCTTTCTCG+TGG - chr4_3:52322215-52322234 Msa0646540:three_prime_UTR 40.0%
TCCACGAGAAAGAAAAAGAG+AGG + chr4_3:52322219-52322238 None:intergenic 40.0%
! TGTTATCGGTTATGTTGAGG+TGG + chr4_3:52322324-52322343 None:intergenic 40.0%
! GGTTGTTATCGGTTATGTTG+AGG + chr4_3:52322327-52322346 None:intergenic 40.0%
CAAACATGTCAACTATTCGG+TGG + chr4_3:52322729-52322748 None:intergenic 40.0%
CCGAATAGTTGACATGTTTG+AGG - chr4_3:52322729-52322748 Msa0646540:intron 40.0%
!! CCCCTTTTTGTCTGTTTTTC+AGG - chr4_3:52322772-52322791 Msa0646540:intron 40.0%
GGAAATGGACCAAGTGAAAA+TGG - chr4_3:52323256-52323275 Msa0646540:intron 40.0%
CTCATATCAACCCTCGATAA+AGG - chr4_3:52323303-52323322 Msa0646540:intron 40.0%
GTTACCGTGGTTAGACATAA+GGG - chr4_3:52323330-52323349 Msa0646540:intron 40.0%
AACACCCTTATGTCTAACCA+CGG + chr4_3:52323337-52323356 None:intergenic 40.0%
GATTCCAACCCTTCTCAAAA+GGG - chr4_3:52323486-52323505 Msa0646540:CDS 40.0%
AACCCTTCTCAAAAGGGATA+GGG - chr4_3:52323492-52323511 Msa0646540:CDS 40.0%
GGATAGGGTGGATAGAAAAT+CGG - chr4_3:52323507-52323526 Msa0646540:CDS 40.0%
TCCAGGCACAAAGTTCTTTA+AGG - chr4_3:52323711-52323730 Msa0646540:CDS 40.0%
TCCTTAAAGAACTTTGTGCC+TGG + chr4_3:52323715-52323734 None:intergenic 40.0%
ATTGTTGGAAGAGTAGAGTC+CGG + chr4_3:52323754-52323773 None:intergenic 40.0%
CTCTACTCTTCCAACAATTG+AGG - chr4_3:52323756-52323775 Msa0646540:CDS 40.0%
CAATTGAGGTCTATATTGCC+GGG - chr4_3:52323770-52323789 Msa0646540:CDS 40.0%
AATAGTGTATCGCAGAACAG+TGG + chr4_3:52324111-52324130 None:intergenic 40.0%
GTTCTTAAATAGTGGCGCTA+TGG - chr4_3:52324142-52324161 Msa0646540:five_prime_UTR 40.0%
GCTATGGCATTGTACTTTAG+TGG - chr4_3:52324158-52324177 Msa0646540:five_prime_UTR 40.0%
! AGGTCTAGCGTTGCTTTAAT+TGG - chr4_3:52324226-52324245 Msa0646540:intron 40.0%
GGAGTCGTTTGAATATGACA+TGG - chr4_3:52324265-52324284 Msa0646540:intron 40.0%
TTTGAGAAATAGCTGTCCTC+CGG - chr4_3:52324439-52324458 Msa0646540:intron 40.0%
CGTATGCTGTTCTTGTGTAT+AGG + chr4_3:52324693-52324712 None:intergenic 40.0%
GAACTTTCATGGTTTAACGC+CGG - chr4_3:52324730-52324749 Msa0646540:intron 40.0%
AGAATAGCCGTCTTCTGAAT+TGG + chr4_3:52324849-52324868 None:intergenic 40.0%
AGATAAAGATGGCCGAACTA+AGG + chr4_3:52324932-52324951 None:intergenic 40.0%
TAGTTCGGCCATCTTTATCT+TGG - chr4_3:52324932-52324951 Msa0646540:five_prime_UTR 40.0%
TCTTTGGGCCAAGATAAAGA+TGG + chr4_3:52324943-52324962 None:intergenic 40.0%
TATTCCCCTACTTATTGAGG+TGG - chr4_3:52325107-52325126 Msa0646540:five_prime_UTR 40.0%
! AATCATGGTGGAGAGACTAA+TGG + chr4_3:52325186-52325205 None:intergenic 40.0%
GTGGTAAGAGAAGATGTGGA+TGG + chr4_3:52322305-52322324 None:intergenic 45.0%
TGAGGTGGTAAGAGAAGATG+TGG + chr4_3:52322309-52322328 None:intergenic 45.0%
AAAGTAGAACTGTCTCGCGA+TGG - chr4_3:52323213-52323232 Msa0646540:intron 45.0%
CGCGATGGAGAATTCGTATA+AGG - chr4_3:52323228-52323247 Msa0646540:intron 45.0%
GGTAACGAACCCTTTATCGA+GGG + chr4_3:52323316-52323335 None:intergenic 45.0%
CGTTACCGTGGTTAGACATA+AGG - chr4_3:52323329-52323348 Msa0646540:intron 45.0%
AAGTGTGAGAATCTCAGGAG+TGG + chr4_3:52323395-52323414 None:intergenic 45.0%
GGATTCCAACCCTTCTCAAA+AGG - chr4_3:52323485-52323504 Msa0646540:CDS 45.0%
!! CTATCCCTTTTGAGAAGGGT+TGG + chr4_3:52323493-52323512 None:intergenic 45.0%
CAACCCTTCTCAAAAGGGAT+AGG - chr4_3:52323491-52323510 Msa0646540:CDS 45.0%
!! CACCCTATCCCTTTTGAGAA+GGG + chr4_3:52323497-52323516 None:intergenic 45.0%
! GAGTGGTAGTGTGAAGTTTG+AGG - chr4_3:52323567-52323586 Msa0646540:CDS 45.0%
! GGAGAAGCAATTGTTGTTGC+CGG - chr4_3:52323732-52323751 Msa0646540:CDS 45.0%
! TCTGGAATTGCATTCAACGC+CGG + chr4_3:52323868-52323887 None:intergenic 45.0%
GACTCCGTGTTGTCAAATTG+CGG - chr4_3:52324046-52324065 Msa0646540:CDS 45.0%
AGTGCCGCAATTTGACAACA+CGG + chr4_3:52324053-52324072 None:intergenic 45.0%
ACTATAGCGCTATGCCCTTT+AGG - chr4_3:52324070-52324089 Msa0646540:CDS 45.0%
CTATAGCGCTATGCCCTTTA+GGG - chr4_3:52324071-52324090 Msa0646540:CDS 45.0%
GACTGATGTCACAACCTAGT+AGG - chr4_3:52324314-52324333 Msa0646540:intron 45.0%
TGTCACAACCTAGTAGGAGA+TGG - chr4_3:52324320-52324339 Msa0646540:intron 45.0%
ACAAGAACAGCATACGCTGA+CGG - chr4_3:52324697-52324716 Msa0646540:intron 45.0%
! ATGGTTTAACGCCGGTGTTA+GGG - chr4_3:52324738-52324757 Msa0646540:intron 45.0%
!! GTTAGGGTTGGTGTTGGTAT+TGG - chr4_3:52324754-52324773 Msa0646540:intron 45.0%
GTTTGTCTTGGAATCGGCAT+TGG - chr4_3:52324784-52324803 Msa0646540:intron 45.0%
! AAATCATACCAAGGTGCCAC+CGG - chr4_3:52324823-52324842 Msa0646540:intron 45.0%
! GTCTTGTTCATCGAGTGCAA+AGG - chr4_3:52324975-52324994 Msa0646540:five_prime_UTR 45.0%
TTGCATGTGTCGTGTTTCGC+CGG - chr4_3:52322598-52322617 Msa0646540:CDS 50.0%
CGGTAACGAACCCTTTATCG+AGG + chr4_3:52323317-52323336 None:intergenic 50.0%
CGATAAAGGGTTCGTTACCG+TGG - chr4_3:52323317-52323336 Msa0646540:intron 50.0%
!! CCACCCTATCCCTTTTGAGA+AGG + chr4_3:52323498-52323517 None:intergenic 50.0%
CCTTCTCAAAAGGGATAGGG+TGG - chr4_3:52323495-52323514 Msa0646540:CDS 50.0%
TATATTGCCGGGTCCTTCTC+TGG - chr4_3:52323781-52323800 Msa0646540:CDS 50.0%
TGACAACACGGAGTCACACA+TGG + chr4_3:52324041-52324060 None:intergenic 50.0%
GCGAAGATGGGGAACTTTCA+TGG - chr4_3:52324719-52324738 Msa0646540:intron 50.0%
CATGGTTTAACGCCGGTGTT+AGG - chr4_3:52324737-52324756 Msa0646540:intron 50.0%
! TTTAACGCCGGTGTTAGGGT+TGG - chr4_3:52324742-52324761 Msa0646540:intron 50.0%
ACCAACACCAACCCTAACAC+CGG + chr4_3:52324752-52324771 None:intergenic 50.0%
! CTTGGAATCGGCATTGGAGT+TGG - chr4_3:52324790-52324809 Msa0646540:intron 50.0%
TAGCCGTCTTCTGAATTGGC+CGG + chr4_3:52324845-52324864 None:intergenic 50.0%
! GATGATCGGAGTGCCAGAGA+AGG + chr4_3:52323797-52323816 None:intergenic 55.0%
TCCTTCTGCGCTTGCTTTGC+AGG - chr4_3:52323915-52323934 Msa0646540:CDS 55.0%
ACCTGCAAAGCAAGCGCAGA+AGG + chr4_3:52323919-52323938 None:intergenic 55.0%
CATACGCTGACGGCGAAGAT+GGG - chr4_3:52324707-52324726 Msa0646540:intron 55.0%
ATACGCTGACGGCGAAGATG+GGG - chr4_3:52324708-52324727 Msa0646540:intron 55.0%
CCACCGGCCAATTCAGAAGA+CGG - chr4_3:52324839-52324858 Msa0646540:intron 55.0%
GCATACGCTGACGGCGAAGA+TGG - chr4_3:52324706-52324725 Msa0646540:intron 60.0%
!! GCCGGTGTTAGGGTTGGTGT+TGG - chr4_3:52324748-52324767 Msa0646540:intron 60.0%
TGAATTGGCCGGTGGCACCT+TGG + chr4_3:52324834-52324853 None:intergenic 60.0%
CCGTCTTCTGAATTGGCCGG+TGG + chr4_3:52324842-52324861 None:intergenic 60.0%
! CGGAGTGCCAGAGAAGGACC+CGG + chr4_3:52323791-52323810 None:intergenic 65.0%
TGCATGTGCTCTCGAGCCGG+AGG + chr4_3:52324458-52324477 None:intergenic 65.0%
! TCCGGCTCGAGAGCACATGC+AGG - chr4_3:52324457-52324476 Msa0646540:intron 65.0%
GCCTGCATGTGCTCTCGAGC+CGG + chr4_3:52324461-52324480 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 52322078 52325284 52322078 ID=Msa0646540;Name=Msa0646540
chr4_3 mRNA 52322078 52325284 52322078 ID=Msa0646540-mRNA-1;Parent=Msa0646540;Name=Msa0646540-mRNA-1;_AED=0.17;_eAED=0.17;_QI=516|1|1|1|0.5|0.33|3|414|310
chr4_3 exon 52322078 52322719 52322078 ID=Msa0646540-mRNA-1:exon:12842;Parent=Msa0646540-mRNA-1
chr4_3 exon 52323426 52324220 52323426 ID=Msa0646540-mRNA-1:exon:12841;Parent=Msa0646540-mRNA-1
chr4_3 exon 52324859 52325284 52324859 ID=Msa0646540-mRNA-1:exon:12840;Parent=Msa0646540-mRNA-1
chr4_3 five_prime_UTR 52324859 52325284 52324859 ID=Msa0646540-mRNA-1:five_prime_utr;Parent=Msa0646540-mRNA-1
chr4_3 five_prime_UTR 52324131 52324220 52324131 ID=Msa0646540-mRNA-1:five_prime_utr;Parent=Msa0646540-mRNA-1
chr4_3 CDS 52323426 52324130 52323426 ID=Msa0646540-mRNA-1:cds;Parent=Msa0646540-mRNA-1
chr4_3 CDS 52322492 52322719 52322492 ID=Msa0646540-mRNA-1:cds;Parent=Msa0646540-mRNA-1
chr4_3 three_prime_UTR 52322078 52322491 52322078 ID=Msa0646540-mRNA-1:three_prime_utr;Parent=Msa0646540-mRNA-1
Gene Sequence

>Msa0646540

ATGGAGAATTCGTATAAGGTTAATGGAAATGGACCAAGTGAAAATGGATACTCTATTGCTAGGCACACGCACTCATATCAACCCTCGATAAAGGGTTCGTTACCGTGGTTAGACATAAGGGTGTTTTATGTAAGGGTTTGCAAGTGTGAGCTTGATAATTCCACTCCTGAGATTCTCACACTTAATCATGTTCCTTTAAATCCTGATACCCTTCTTGAAGTTAATGGTGTTCGGAGTAGTTTATATTCTGATGGGATTCCAACCCTTCTCAAAAGGGATAGGGTGGATAGAAAATCGGAAGAAGTTACGTTTGTAAGCACTGATAGTATAAGGATGAGTGGTAGTGTGAAGTTTGAGGTGTTTGATAAAGATAAGCTTCTTCTGTTTGGTACCTTAGAATTATGTAACAGCAATGGTGTTGTTAGAGAATCAAATTCTAATGGACAAAGTTGGAGCATGAAGTGTGAGTCGAATATAATTCCAGGCACAAAGTTCTTTAAGGAGAAGCAATTGTTGTTGCCGGACTCTACTCTTCCAACAATTGAGGTCTATATTGCCGGGTCCTTCTCTGGCACTCCGATCATCTTAACGAAGACCTTGCATCTTAGTTCTCAAAAAAGACATACAAGGAAGCCGGCGTTGAATGCAATTCCAGAGAATGATGCAAATGAAAATGGGAAAGGTCCTTCTGCGCTTGCTTTGCAGGCTCCAGATTACATGTATGACAAACATGATGATGAGGATTATCACGGCCTATACACAAGAACAGCATACGCTGACGGCGAAGATGGGGAACTTTCATGGTTTAACGCCGGTGTTAGGGTTGGTGTTGGTATTGGACTTAGTGTTTGTCTTGGAATCGGCATTGGAGTTGGCATACTCGTTAAATCATACCAAGGTGCCACCGGCCAATTCAGAAGACGGCTATTCTAA
Protein sequence

>Msa0646540

MENSYKVNGNGPSENGYSIARHTHSYQPSIKGSLPWLDIRVFYVRVCKCELDNSTPEILTLNHVPLNPDTLLEVNGVRSSLYSDGIPTLLKRDRVDRKSEEVTFVSTDSIRMSGSVKFEVFDKDKLLLFGTLELCNSNGVVRESNSNGQSWSMKCESNIIPGTKFFKEKQLLLPDSTLPTIEVYIAGSFSGTPIILTKTLHLSSQKRHTRKPALNAIPENDANENGKGPSALALQAPDYMYDKHDDEDYHGLYTRTAYADGEDGELSWFNAGVRVGVGIGLSVCLGIGIGVGILVKSYQGATGQFRRRLF*