AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0550080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550080 MtrunA17_Chr4g0034951 98.071 311 5 1 1 310 1 311 0.0 627
Msa0550080 MtrunA17_Chr1g0155081 49.320 294 127 7 26 309 30 311 7.55e-91 273
Msa0550080 MtrunA17_Chr8g0348371 41.853 313 154 9 14 310 3 303 1.39e-65 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550080 AT1G19400.1 54.485 301 127 5 13 310 10 303 3.40e-107 314
Msa0550080 AT1G19400.3 54.485 301 127 5 13 310 10 303 3.40e-107 314
Msa0550080 AT1G19400.2 54.485 301 127 5 13 310 10 303 3.40e-107 314
Msa0550080 AT1G75180.1 56.569 274 115 3 41 310 42 315 2.81e-106 313
Msa0550080 AT1G75180.3 56.569 274 115 3 41 310 42 315 2.81e-106 313
Msa0550080 AT1G19400.4 54.185 227 98 3 13 236 10 233 1.73e-78 239
Msa0550080 AT1G75180.2 55.959 193 83 1 41 231 42 234 5.54e-72 223
Msa0550080 AT1G75180.4 55.959 193 83 1 41 231 42 234 5.25e-71 221
Msa0550080 AT1G01500.1 41.892 296 141 10 36 310 42 327 3.91e-63 202

Find 133 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGGCACAAAGTTCTTTA+AGG 0.155540 4_1:-45499469 Msa0550080:CDS
AGGTGGTAATAAATGATTTA+AGG 0.188726 4_1:-45498056 Msa0550080:three_prime_UTR
ATGGATATTGTTTAATTTAA+TGG 0.209398 4_1:+45500898 None:intergenic
GATACCCTTCTTGAAGTTAA+TGG 0.212761 4_1:-45499744 Msa0550080:CDS
GAGAATGATGCAAATGAAAA+TGG 0.217056 4_1:-45499294 Msa0550080:CDS
TCCTTCTGCGCTTGCTTTGC+AGG 0.233001 4_1:-45499265 Msa0550080:intron
CTTTAAGCTTCAATAGATTC+TGG 0.234279 4_1:-45500798 Msa0550080:five_prime_UTR
AGACATTAACATGTTGTCTT+TGG 0.250262 4_1:+45498224 None:intergenic
CATGGTTTAACGCCGGTGTT+AGG 0.255714 4_1:-45498443 Msa0550080:CDS
AAGAACAAATCTTGCAATCT+TGG 0.266859 4_1:+45500818 None:intergenic
GAGAATTCGTATAAGGTTAA+TGG 0.283457 4_1:-45499945 Msa0550080:CDS
GGAAATGGACCAAGTGAAAA+TGG 0.283915 4_1:-45499924 Msa0550080:CDS
GTTAGGGTTGGTGTTGGTAT+TGG 0.297925 4_1:-45498426 Msa0550080:CDS
ATTGCTGTTACATAATTCTA+AGG 0.316159 4_1:+45499557 None:intergenic
GGATATATATTATATAGTTT+TGG 0.316791 4_1:-45498184 Msa0550080:three_prime_UTR
CTCATATCAACCCTCGATAA+AGG 0.321091 4_1:-45499877 Msa0550080:CDS
AGAAGGGTATCAGGATTTAA+AGG 0.331368 4_1:+45499756 None:intergenic
GGAGAAGCAATTGTTGTTGC+CGG 0.344534 4_1:-45499448 Msa0550080:CDS
GACATTAACATGTTGTCTTT+GGG 0.346532 4_1:+45498225 None:intergenic
AAAGATAAGCTTCTTCTGTT+TGG 0.350138 4_1:-45499582 Msa0550080:CDS
TTAACTTCAAGAAGGGTATC+AGG 0.353127 4_1:+45499747 None:intergenic
ATCAAAACATAGGTTGTTAT+CGG 0.360179 4_1:+45500846 None:intergenic
TCGTATAAGGTTAATGGAAA+TGG 0.363071 4_1:-45499939 Msa0550080:CDS
GGATCGATTACTTGCAAGAT+TGG 0.371568 4_1:+45501095 None:intergenic
GCGAAGATGGGGAACTTTCA+TGG 0.371984 4_1:-45498461 Msa0550080:CDS
AGAATAGCCGTCTTCTGAAT+TGG 0.372025 4_1:+45498334 None:intergenic
AGAACAAATCTTGCAATCTT+GGG 0.374948 4_1:+45500819 None:intergenic
GTTAGAGAATCAAATTCTAA+TGG 0.378979 4_1:-45499528 Msa0550080:CDS
TATATTGCCGGGTCCTTCTC+TGG 0.379377 4_1:-45499399 Msa0550080:CDS
TAGTTCGGCCATCTTTATCT+TGG 0.380101 4_1:-45498248 Msa0550080:three_prime_UTR
CGTTACCGTGGTTAGACATA+AGG 0.381604 4_1:-45499851 Msa0550080:CDS
TGATCAACATTTATAAAGTA+AGG 0.389780 4_1:-45497924 Msa0550080:three_prime_UTR
ACAATTGAGGTCTATATTGC+CGG 0.393367 4_1:-45499411 Msa0550080:CDS
TTGTAAGCACTGATAGTATA+AGG 0.394406 4_1:-45499638 Msa0550080:CDS
TTTAAGCTTCAATAGATTCT+GGG 0.398434 4_1:-45500797 Msa0550080:five_prime_UTR
TGTTTGTCATACATGTAATC+TGG 0.402010 4_1:+45498534 None:intergenic
GAAAACTGAAATGTTATGTT+GGG 0.402948 4_1:+45501050 None:intergenic
GGTGGTAATAAATGATTTAA+GGG 0.404373 4_1:-45498055 Msa0550080:three_prime_UTR
CGCGATGGAGAATTCGTATA+AGG 0.406902 4_1:-45499952 Msa0550080:exon
TACTTTATAAATGTTGATCA+TGG 0.417685 4_1:+45497927 None:intergenic
TTTGTGTGTGAATTAATTAG+AGG 0.422085 4_1:+45500993 None:intergenic
TTCTTGAAGTTAATGGTGTT+CGG 0.422504 4_1:-45499737 Msa0550080:CDS
CTTAGTGTTTGTCTTGGAAT+CGG 0.423405 4_1:-45498402 Msa0550080:CDS
TGATTAAGTGTGAGAATCTC+AGG 0.428692 4_1:+45499783 None:intergenic
CGGAGTAGTTTATATTCTGA+TGG 0.435949 4_1:-45499717 Msa0550080:CDS
GCAATATAGACCTCAATTGT+TGG 0.437528 4_1:+45499414 None:intergenic
TTATATTCCCCTACTTATTG+AGG 0.438498 4_1:-45498076 Msa0550080:three_prime_UTR
CTTGGAATCGGCATTGGAGT+TGG 0.441010 4_1:-45498390 Msa0550080:CDS
TTATTACCACCTCAATAAGT+AGG 0.441077 4_1:+45498067 None:intergenic
GGATTCCAACCCTTCTCAAA+AGG 0.443968 4_1:-45499695 Msa0550080:CDS
TCCTTAAAGAACTTTGTGCC+TGG 0.447833 4_1:+45499468 None:intergenic
GGATAGGGTGGATAGAAAAT+CGG 0.450168 4_1:-45499673 Msa0550080:CDS
GTTTGTCTTGGAATCGGCAT+TGG 0.451016 4_1:-45498396 Msa0550080:CDS
TGTGAGTCGAATATAATTCC+AGG 0.452189 4_1:-45499486 Msa0550080:CDS
TGAAAACTGAAATGTTATGT+TGG 0.452656 4_1:+45501049 None:intergenic
AAGAATAATATCAGATTCAA+AGG 0.458806 4_1:-45500933 Msa0550080:five_prime_UTR
ATGGTTTAACGCCGGTGTTA+GGG 0.458989 4_1:-45498442 Msa0550080:CDS
GAATTAATTAGAGGAAGAAA+AGG 0.460023 4_1:+45501002 None:intergenic
TCATATCAACCCTCGATAAA+GGG 0.470195 4_1:-45499876 Msa0550080:CDS
AGAATGATGCAAATGAAAAT+GGG 0.470852 4_1:-45499293 Msa0550080:CDS
CGTATGCTGTTCTTGTGTAT+AGG 0.473133 4_1:+45498490 None:intergenic
TGAATTGGCCGGTGGCACCT+TGG 0.475731 4_1:+45498349 None:intergenic
CAACCCTTCTCAAAAGGGAT+AGG 0.480345 4_1:-45499689 Msa0550080:CDS
GGGTGGTAGTGTGAAGTTTG+AGG 0.481054 4_1:-45499613 Msa0550080:CDS
ATTGGACTTAGTGTTTGTCT+TGG 0.481547 4_1:-45498408 Msa0550080:CDS
TATTACCACCTCAATAAGTA+GGG 0.486522 4_1:+45498068 None:intergenic
GCATACGCTGACGGCGAAGA+TGG 0.492307 4_1:-45498474 Msa0550080:CDS
CAAATTCTAATGGACAAAGT+TGG 0.492533 4_1:-45499518 Msa0550080:CDS
GTTTAATTTAATGGAAGATG+TGG 0.493650 4_1:+45500907 None:intergenic
AGATAAAGATGGCCGAACTA+AGG 0.499057 4_1:+45498251 None:intergenic
GTTACCGTGGTTAGACATAA+GGG 0.505953 4_1:-45499850 Msa0550080:CDS
CATACGCTGACGGCGAAGAT+GGG 0.511131 4_1:-45498473 Msa0550080:CDS
GATTCCAACCCTTCTCAAAA+GGG 0.526688 4_1:-45499694 Msa0550080:CDS
GGAGTAGTTTATATTCTGAT+GGG 0.528712 4_1:-45499716 Msa0550080:CDS
GATCAACATTTATAAAGTAA+GGG 0.530296 4_1:-45497923 Msa0550080:three_prime_UTR
ACCTGCAAAGCAAGCGCAGA+AGG 0.534939 4_1:+45499264 None:intergenic
AGAATAATATCAGATTCAAA+GGG 0.538270 4_1:-45500932 Msa0550080:five_prime_UTR
AAAGGAGAGTGTGAAATTGT+TGG 0.539146 4_1:+45501020 None:intergenic
GGTTGTTATCGGTTATGTTG+AGG 0.539393 4_1:+45500857 None:intergenic
CAAAGAAACTGAGAAAATCA+TGG 0.543052 4_1:+45497982 None:intergenic
AGTTAACTTTGTTGTTGCAG+TGG 0.548009 4_1:-45500031 Msa0550080:five_prime_UTR
AACCCTTCTCAAAAGGGATA+GGG 0.548703 4_1:-45499688 Msa0550080:CDS
GCCGGTGTTAGGGTTGGTGT+TGG 0.549235 4_1:-45498432 Msa0550080:CDS
AAGCACTGATAGTATAAGGA+TGG 0.550177 4_1:-45499634 Msa0550080:CDS
AATCATGGTGGAGAGACTAA+TGG 0.550185 4_1:+45497997 None:intergenic
CTTGGGATGAATCAAAACAT+AGG 0.551006 4_1:+45500836 None:intergenic
CTCTACTCTTCCAACAATTG+AGG 0.556408 4_1:-45499424 Msa0550080:CDS
TATTCCCCTACTTATTGAGG+TGG 0.561142 4_1:-45498073 Msa0550080:three_prime_UTR
TGAGGTGGTAAGAGAAGATG+TGG 0.564391 4_1:+45500875 None:intergenic
GTGGTAATAAATGATTTAAG+GGG 0.565067 4_1:-45498054 Msa0550080:three_prime_UTR
AAATCATACCAAGGTGCCAC+CGG 0.568352 4_1:-45498357 Msa0550080:CDS
GAACACCATTAACTTCAAGA+AGG 0.569037 4_1:+45499739 None:intergenic
TTTAACGCCGGTGTTAGGGT+TGG 0.570434 4_1:-45498438 Msa0550080:CDS
TGTTATCGGTTATGTTGAGG+TGG 0.575774 4_1:+45500860 None:intergenic
TTTAATTTAATGGAAGATGT+GGG 0.578479 4_1:+45500908 None:intergenic
TAGCCGTCTTCTGAATTGGC+CGG 0.581948 4_1:+45498338 None:intergenic
CAATTGAGGTCTATATTGCC+GGG 0.582339 4_1:-45499410 Msa0550080:CDS
ATCAACATTTATAAAGTAAG+GGG 0.586146 4_1:-45497922 Msa0550080:three_prime_UTR
CCACCGGCCAATTCAGAAGA+CGG 0.588469 4_1:-45498341 Msa0550080:CDS
TCTGGAATTGCATTCAACGC+CGG 0.588676 4_1:+45499315 None:intergenic
AAAATGGATACTCTATTGCT+AGG 0.589815 4_1:-45499908 Msa0550080:CDS
CGATAAAGGGTTCGTTACCG+TGG 0.592561 4_1:-45499863 Msa0550080:CDS
AAGGTCTTCGTTAAGATGAT+CGG 0.592566 4_1:+45499372 None:intergenic
CGGAGTGCCAGAGAAGGACC+CGG 0.592882 4_1:+45499392 None:intergenic
GATGCAAATGAAAATGGGAA+AGG 0.596141 4_1:-45499288 Msa0550080:CDS
AACACCATTAACTTCAAGAA+GGG 0.596194 4_1:+45499740 None:intergenic
CCTTCTCAAAAGGGATAGGG+TGG 0.600087 4_1:-45499685 Msa0550080:CDS
ATTGTTGGAAGAGTAGAGTC+CGG 0.603903 4_1:+45499429 None:intergenic
TGGTAATAAATGATTTAAGG+GGG 0.603940 4_1:-45498053 Msa0550080:three_prime_UTR
GTGGTAAGAGAAGATGTGGA+TGG 0.604928 4_1:+45500879 None:intergenic
ACAAGAACAGCATACGCTGA+CGG 0.606360 4_1:-45498483 Msa0550080:CDS
TCTTTGGGCCAAGATAAAGA+TGG 0.610464 4_1:+45498240 None:intergenic
AAAGTAGAACTGTCTCGCGA+TGG 0.611108 4_1:-45499967 Msa0550080:exon
AGCACTGATAGTATAAGGAT+GGG 0.617470 4_1:-45499633 Msa0550080:CDS
ACCAACACCAACCCTAACAC+CGG 0.618909 4_1:+45498431 None:intergenic
AAAACTGAAATGTTATGTTG+GGG 0.627111 4_1:+45501051 None:intergenic
CGGTAACGAACCCTTTATCG+AGG 0.628590 4_1:+45499866 None:intergenic
ATACGCTGACGGCGAAGATG+GGG 0.629010 4_1:-45498472 Msa0550080:CDS
CATGATGATGAGGATTATCA+CGG 0.635768 4_1:-45498513 Msa0550080:CDS
GATGATCGGAGTGCCAGAGA+AGG 0.637274 4_1:+45499386 None:intergenic
CCGTCTTCTGAATTGGCCGG+TGG 0.642624 4_1:+45498341 None:intergenic
GGTAACGAACCCTTTATCGA+GGG 0.642976 4_1:+45499867 None:intergenic
ACTGATAGTATAAGGATGGG+TGG 0.646255 4_1:-45499630 Msa0550080:CDS
AACACCCTTATGTCTAACCA+CGG 0.647619 4_1:+45499846 None:intergenic
TTAGAATTATGTAACAGCAA+TGG 0.648523 4_1:-45499555 Msa0550080:CDS
GAACTTTCATGGTTTAACGC+CGG 0.650464 4_1:-45498450 Msa0550080:CDS
ATTACCACCTCAATAAGTAG+GGG 0.652127 4_1:+45498069 None:intergenic
AAGTGTGAGAATCTCAGGAG+TGG 0.662685 4_1:+45499788 None:intergenic
GTATGACAAACATGATGATG+AGG 0.669080 4_1:-45498523 Msa0550080:CDS
GTCTTGTTCATCGAGTGCAA+AGG 0.669479 4_1:-45498205 Msa0550080:three_prime_UTR
TGATTCTTACAGTAATCACA+AGG 0.698127 4_1:-45498022 Msa0550080:three_prime_UTR
ATACTCGTTAAATCATACCA+AGG 0.711189 4_1:-45498366 Msa0550080:CDS
AGAAACTGAGAAAATCATGG+TGG 0.715746 4_1:+45497985 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAATTTGATTTTTAATTT+TGG - chr4_1:45498304-45498323 Msa0550080:three_prime_UTR 10.0%
!!! ATGGATATTGTTTAATTTAA+TGG + chr4_1:45498125-45498144 None:intergenic 15.0%
!! CAAAGAAATATAAGAATTAA+AGG + chr4_1:45498690-45498709 None:intergenic 15.0%
!! TTTACAAAAGAAAATCAAAA+AGG + chr4_1:45498957-45498976 None:intergenic 15.0%
!!! AAATTTTTAAAATCTTGTCA+AGG + chr4_1:45500369-45500388 None:intergenic 15.0%
!! AGCATTTACATAAAATAATA+AGG + chr4_1:45500459-45500478 None:intergenic 15.0%
!!! GGATATATATTATATAGTTT+TGG - chr4_1:45500836-45500855 Msa0550080:five_prime_UTR 15.0%
!! AAGAATAATATCAGATTCAA+AGG - chr4_1:45498087-45498106 Msa0550080:three_prime_UTR 20.0%
!! AGAATAATATCAGATTCAAA+GGG - chr4_1:45498088-45498107 Msa0550080:three_prime_UTR 20.0%
!!! TTTAATTTAATGGAAGATGT+GGG + chr4_1:45498115-45498134 None:intergenic 20.0%
!!! AAATTTTGATTATTGTTACG+TGG + chr4_1:45498292-45498311 None:intergenic 20.0%
!!! TTGAATTTTGCATTAGTTAA+TGG - chr4_1:45498372-45498391 Msa0550080:CDS 20.0%
!! CAATCAGATAAAAATAATAC+AGG + chr4_1:45498759-45498778 None:intergenic 20.0%
!!! TATTTTTATCTGATTGAGTA+AGG - chr4_1:45498763-45498782 Msa0550080:intron 20.0%
!!! ATTTATTTGTGCTTTTATGT+TGG - chr4_1:45498888-45498907 Msa0550080:intron 20.0%
!! ATTTAAATAAGCACATGAAA+AGG + chr4_1:45499804-45499823 None:intergenic 20.0%
!! GATATAACTGTAAATTATCT+TGG - chr4_1:45500199-45500218 Msa0550080:intron 20.0%
!! ATATTTATGAACAAACCAAA+TGG - chr4_1:45500389-45500408 Msa0550080:intron 20.0%
!!! GGAATTATCTATAATTTGTT+GGG + chr4_1:45500438-45500457 None:intergenic 20.0%
!!! AGGAATTATCTATAATTTGT+TGG + chr4_1:45500439-45500458 None:intergenic 20.0%
!! TACTTTATAAATGTTGATCA+TGG + chr4_1:45501096-45501115 None:intergenic 20.0%
!! TGATCAACATTTATAAAGTA+AGG - chr4_1:45501096-45501115 Msa0550080:five_prime_UTR 20.0%
!! GATCAACATTTATAAAGTAA+GGG - chr4_1:45501097-45501116 Msa0550080:five_prime_UTR 20.0%
!! ATCAACATTTATAAAGTAAG+GGG - chr4_1:45501098-45501117 Msa0550080:five_prime_UTR 20.0%
! AAAACTGAAATGTTATGTTG+GGG + chr4_1:45497972-45497991 None:intergenic 25.0%
! GAAAACTGAAATGTTATGTT+GGG + chr4_1:45497973-45497992 None:intergenic 25.0%
! TGAAAACTGAAATGTTATGT+TGG + chr4_1:45497974-45497993 None:intergenic 25.0%
! GAATTAATTAGAGGAAGAAA+AGG + chr4_1:45498021-45498040 None:intergenic 25.0%
! TTTGTGTGTGAATTAATTAG+AGG + chr4_1:45498030-45498049 None:intergenic 25.0%
!! GTTTAATTTAATGGAAGATG+TGG + chr4_1:45498116-45498135 None:intergenic 25.0%
! ATCAAAACATAGGTTGTTAT+CGG + chr4_1:45498177-45498196 None:intergenic 25.0%
! TTTAAGCTTCAATAGATTCT+GGG - chr4_1:45498223-45498242 Msa0550080:three_prime_UTR 25.0%
! ATTTAGACAAAAAAAACAGC+CGG + chr4_1:45498460-45498479 None:intergenic 25.0%
! GAAAATCAAAAAGGTTGAAA+AGG + chr4_1:45498948-45498967 None:intergenic 25.0%
!! ATTGCTGTTACATAATTCTA+AGG + chr4_1:45499466-45499485 None:intergenic 25.0%
! TTAGAATTATGTAACAGCAA+TGG - chr4_1:45499465-45499484 Msa0550080:CDS 25.0%
! GTTAGAGAATCAAATTCTAA+TGG - chr4_1:45499492-45499511 Msa0550080:CDS 25.0%
! AGAATGATGCAAATGAAAAT+GGG - chr4_1:45499727-45499746 Msa0550080:CDS 25.0%
!! TACAAAGTGTTCTTAAATAG+TGG - chr4_1:45499974-45499993 Msa0550080:five_prime_UTR 25.0%
! TTCTTCATAAGATTTCCATT+TGG + chr4_1:45500407-45500426 None:intergenic 25.0%
!!! TCTATAATTTGTTGGGAAAA+TGG + chr4_1:45500431-45500450 None:intergenic 25.0%
!!! TTATTTTATGTAAATGCTGC+AGG - chr4_1:45500461-45500480 Msa0550080:intron 25.0%
! AGGTGGTAATAAATGATTTA+AGG - chr4_1:45500964-45500983 Msa0550080:five_prime_UTR 25.0%
! GGTGGTAATAAATGATTTAA+GGG - chr4_1:45500965-45500984 Msa0550080:five_prime_UTR 25.0%
! GTGGTAATAAATGATTTAAG+GGG - chr4_1:45500966-45500985 Msa0550080:five_prime_UTR 25.0%
! TGGTAATAAATGATTTAAGG+GGG - chr4_1:45500967-45500986 Msa0550080:five_prime_UTR 25.0%
!!! AACATTTTTTGTCTGAGAAA+AGG + chr4_1:45501064-45501083 None:intergenic 25.0%
!!! TTTTCTGTTTGCTGTATAAG+GGG + chr4_1:45497949-45497968 None:intergenic 30.0%
!!! GTTTTCTGTTTGCTGTATAA+GGG + chr4_1:45497950-45497969 None:intergenic 30.0%
AGAACAAATCTTGCAATCTT+GGG + chr4_1:45498204-45498223 None:intergenic 30.0%
AAGAACAAATCTTGCAATCT+TGG + chr4_1:45498205-45498224 None:intergenic 30.0%
CTTTAAGCTTCAATAGATTC+TGG - chr4_1:45498222-45498241 Msa0550080:three_prime_UTR 30.0%
! TTTTGGCACAAGATATTTTG+TGG - chr4_1:45498321-45498340 Msa0550080:exon 30.0%
!! TTAATGGATTGTGTTAGTGA+CGG - chr4_1:45498388-45498407 Msa0550080:CDS 30.0%
CCTCAAACATATCAACTATT+CGG + chr4_1:45498572-45498591 None:intergenic 30.0%
! TATTTGTGCTTTTATGTTGG+TGG - chr4_1:45498891-45498910 Msa0550080:intron 30.0%
GAGAATTCGTATAAGGTTAA+TGG - chr4_1:45499075-45499094 Msa0550080:intron 30.0%
TCGTATAAGGTTAATGGAAA+TGG - chr4_1:45499081-45499100 Msa0550080:intron 30.0%
!! ATAGAGTATCCATTTTCACT+TGG + chr4_1:45499108-45499127 None:intergenic 30.0%
AAAATGGATACTCTATTGCT+AGG - chr4_1:45499112-45499131 Msa0550080:intron 30.0%
!!! ACATAAGGGTGTTTTATGTA+AGG - chr4_1:45499184-45499203 Msa0550080:intron 30.0%
!!! CATAAGGGTGTTTTATGTAA+GGG - chr4_1:45499185-45499204 Msa0550080:intron 30.0%
AACACCATTAACTTCAAGAA+GGG + chr4_1:45499283-45499302 None:intergenic 30.0%
TTCTTGAAGTTAATGGTGTT+CGG - chr4_1:45499283-45499302 Msa0550080:CDS 30.0%
GGAGTAGTTTATATTCTGAT+GGG - chr4_1:45499304-45499323 Msa0550080:CDS 30.0%
!! TTGTAAGCACTGATAGTATA+AGG - chr4_1:45499382-45499401 Msa0550080:CDS 30.0%
AAAGATAAGCTTCTTCTGTT+TGG - chr4_1:45499438-45499457 Msa0550080:CDS 30.0%
! CAAATTCTAATGGACAAAGT+TGG - chr4_1:45499502-45499521 Msa0550080:CDS 30.0%
!! TTTTTGAGAACTAAGATGCA+AGG + chr4_1:45499670-45499689 None:intergenic 30.0%
GTTCTCAAAAAAGACATACA+AGG - chr4_1:45499679-45499698 Msa0550080:CDS 30.0%
! TTTTCATTTGCATCATTCTC+TGG + chr4_1:45499726-45499745 None:intergenic 30.0%
GAGAATGATGCAAATGAAAA+TGG - chr4_1:45499726-45499745 Msa0550080:CDS 30.0%
ATTCAAGCAACAAGTTAACA+AGG + chr4_1:45499856-45499875 None:intergenic 30.0%
! GATTTGTTCAAATCCCTAAA+GGG + chr4_1:45499927-45499946 None:intergenic 30.0%
!! ATTGGCTCAACGTAATTTTA+TGG - chr4_1:45500084-45500103 Msa0550080:intron 30.0%
TGTTTGTCATACATGTAATC+TGG + chr4_1:45500489-45500508 None:intergenic 30.0%
ATACTCGTTAAATCATACCA+AGG - chr4_1:45500654-45500673 Msa0550080:intron 30.0%
AAAAACAGCTAACCTTAGTT+CGG - chr4_1:45500757-45500776 Msa0550080:five_prime_UTR 30.0%
GACATTAACATGTTGTCTTT+GGG + chr4_1:45500798-45500817 None:intergenic 30.0%
AGACATTAACATGTTGTCTT+TGG + chr4_1:45500799-45500818 None:intergenic 30.0%
TTATATTCCCCTACTTATTG+AGG - chr4_1:45500944-45500963 Msa0550080:five_prime_UTR 30.0%
TATTACCACCTCAATAAGTA+GGG + chr4_1:45500955-45500974 None:intergenic 30.0%
TTATTACCACCTCAATAAGT+AGG + chr4_1:45500956-45500975 None:intergenic 30.0%
TGATTCTTACAGTAATCACA+AGG - chr4_1:45500998-45501017 Msa0550080:five_prime_UTR 30.0%
CAAAGAAACTGAGAAAATCA+TGG + chr4_1:45501041-45501060 None:intergenic 30.0%
!!! GGTTTTCTGTTTGCTGTATA+AGG + chr4_1:45497951-45497970 None:intergenic 35.0%
! AAAGGAGAGTGTGAAATTGT+TGG + chr4_1:45498003-45498022 None:intergenic 35.0%
CTTGGGATGAATCAAAACAT+AGG + chr4_1:45498187-45498206 None:intergenic 35.0%
CAAACATATCAACTATTCGG+TGG + chr4_1:45498569-45498588 None:intergenic 35.0%
! CCGAATAGTTGATATGTTTG+AGG - chr4_1:45498569-45498588 Msa0550080:intron 35.0%
CCTGAAAAACAGACAAAAAG+AGG + chr4_1:45498615-45498634 None:intergenic 35.0%
!! CCTCTTTTTGTCTGTTTTTC+AGG - chr4_1:45498612-45498631 Msa0550080:intron 35.0%
!!! TTTTGTCTGTTTTTCAGGTC+TGG - chr4_1:45498617-45498636 Msa0550080:intron 35.0%
!! AGTTAACTTTGTTGTTGCAG+TGG - chr4_1:45498989-45499008 Msa0550080:intron 35.0%
TCATATCAACCCTCGATAAA+GGG - chr4_1:45499144-45499163 Msa0550080:intron 35.0%
TGATTAAGTGTGAGAATCTC+AGG + chr4_1:45499240-45499259 None:intergenic 35.0%
!! AGAAGGGTATCAGGATTTAA+AGG + chr4_1:45499267-45499286 None:intergenic 35.0%
!! TTAACTTCAAGAAGGGTATC+AGG + chr4_1:45499276-45499295 None:intergenic 35.0%
GATACCCTTCTTGAAGTTAA+TGG - chr4_1:45499276-45499295 Msa0550080:CDS 35.0%
GAACACCATTAACTTCAAGA+AGG + chr4_1:45499284-45499303 None:intergenic 35.0%
CGGAGTAGTTTATATTCTGA+TGG - chr4_1:45499303-45499322 Msa0550080:CDS 35.0%
!! AAGCACTGATAGTATAAGGA+TGG - chr4_1:45499386-45499405 Msa0550080:CDS 35.0%
!! AGCACTGATAGTATAAGGAT+GGG - chr4_1:45499387-45499406 Msa0550080:CDS 35.0%
TGTGAGTCGAATATAATTCC+AGG - chr4_1:45499534-45499553 Msa0550080:CDS 35.0%
GCAATATAGACCTCAATTGT+TGG + chr4_1:45499609-45499628 None:intergenic 35.0%
ACAATTGAGGTCTATATTGC+CGG - chr4_1:45499609-45499628 Msa0550080:CDS 35.0%
AAGGTCTTCGTTAAGATGAT+CGG + chr4_1:45499651-45499670 None:intergenic 35.0%
AAAAAGACATACAAGGAAGC+CGG - chr4_1:45499686-45499705 Msa0550080:CDS 35.0%
GATGCAAATGAAAATGGGAA+AGG - chr4_1:45499732-45499751 Msa0550080:CDS 35.0%
! ATGCACTGACATAGTTAAAG+AGG + chr4_1:45499830-45499849 None:intergenic 35.0%
! CGATTTGTTCAAATCCCTAA+AGG + chr4_1:45499928-45499947 None:intergenic 35.0%
TGATATGCGATTGACAATAC+AGG - chr4_1:45500046-45500065 Msa0550080:intron 35.0%
ATTATGTTCCATCTCCTACT+AGG + chr4_1:45500171-45500190 None:intergenic 35.0%
!! TTATCTTGGCTCTTTTTCTG+AGG - chr4_1:45500213-45500232 Msa0550080:intron 35.0%
! AACTATTTTCATCACTCGTC+AGG + chr4_1:45500345-45500364 None:intergenic 35.0%
! GTATGACAAACATGATGATG+AGG - chr4_1:45500497-45500516 Msa0550080:intron 35.0%
! CATGATGATGAGGATTATCA+CGG - chr4_1:45500507-45500526 Msa0550080:intron 35.0%
ATTGGACTTAGTGTTTGTCT+TGG - chr4_1:45500612-45500631 Msa0550080:intron 35.0%
CTTAGTGTTTGTCTTGGAAT+CGG - chr4_1:45500618-45500637 Msa0550080:intron 35.0%
!!! TAGCTGTTTTTGGATCAAGA+GGG + chr4_1:45500748-45500767 None:intergenic 35.0%
!!! TTAGCTGTTTTTGGATCAAG+AGG + chr4_1:45500749-45500768 None:intergenic 35.0%
!! GAACTAAGGTTAGCTGTTTT+TGG + chr4_1:45500758-45500777 None:intergenic 35.0%
ATTACCACCTCAATAAGTAG+GGG + chr4_1:45500954-45500973 None:intergenic 35.0%
ATAAATGATTTAAGGGGGCT+TGG - chr4_1:45500972-45500991 Msa0550080:five_prime_UTR 35.0%
AGAAACTGAGAAAATCATGG+TGG + chr4_1:45501038-45501057 None:intergenic 35.0%
GGATCGATTACTTGCAAGAT+TGG + chr4_1:45497928-45497947 None:intergenic 40.0%
! TCCTCTCTTTTTCTTTCTCG+TGG - chr4_1:45498054-45498073 Msa0550080:three_prime_UTR 40.0%
TCCACGAGAAAGAAAAAGAG+AGG + chr4_1:45498058-45498077 None:intergenic 40.0%
! TGTTATCGGTTATGTTGAGG+TGG + chr4_1:45498163-45498182 None:intergenic 40.0%
! GGTTGTTATCGGTTATGTTG+AGG + chr4_1:45498166-45498185 None:intergenic 40.0%
GGAAATGGACCAAGTGAAAA+TGG - chr4_1:45499096-45499115 Msa0550080:intron 40.0%
CTCATATCAACCCTCGATAA+AGG - chr4_1:45499143-45499162 Msa0550080:intron 40.0%
GTTACCGTGGTTAGACATAA+GGG - chr4_1:45499170-45499189 Msa0550080:intron 40.0%
AACACCCTTATGTCTAACCA+CGG + chr4_1:45499177-45499196 None:intergenic 40.0%
GATTCCAACCCTTCTCAAAA+GGG - chr4_1:45499326-45499345 Msa0550080:CDS 40.0%
AACCCTTCTCAAAAGGGATA+GGG - chr4_1:45499332-45499351 Msa0550080:CDS 40.0%
GGATAGGGTGGATAGAAAAT+CGG - chr4_1:45499347-45499366 Msa0550080:CDS 40.0%
ACTGATAGTATAAGGATGGG+TGG - chr4_1:45499390-45499409 Msa0550080:CDS 40.0%
TCCAGGCACAAAGTTCTTTA+AGG - chr4_1:45499551-45499570 Msa0550080:CDS 40.0%
TCCTTAAAGAACTTTGTGCC+TGG + chr4_1:45499555-45499574 None:intergenic 40.0%
ATTGTTGGAAGAGTAGAGTC+CGG + chr4_1:45499594-45499613 None:intergenic 40.0%
CTCTACTCTTCCAACAATTG+AGG - chr4_1:45499596-45499615 Msa0550080:CDS 40.0%
CAATTGAGGTCTATATTGCC+GGG - chr4_1:45499610-45499629 Msa0550080:CDS 40.0%
AATAGTGTATCGCAGAACAG+TGG + chr4_1:45499951-45499970 None:intergenic 40.0%
GTTCTTAAATAGTGGCGCTA+TGG - chr4_1:45499982-45500001 Msa0550080:five_prime_UTR 40.0%
GCTATGGCATTGTACTTTAG+TGG - chr4_1:45499998-45500017 Msa0550080:five_prime_UTR 40.0%
! AGGTCTAGCGTTGCTTTAAT+TGG - chr4_1:45500066-45500085 Msa0550080:intron 40.0%
GGAGTCGTTTGAATATGACA+TGG - chr4_1:45500105-45500124 Msa0550080:intron 40.0%
TTTGAGAAATAGCTGTCCTC+CGG - chr4_1:45500279-45500298 Msa0550080:intron 40.0%
CGTATGCTGTTCTTGTGTAT+AGG + chr4_1:45500533-45500552 None:intergenic 40.0%
GAACTTTCATGGTTTAACGC+CGG - chr4_1:45500570-45500589 Msa0550080:intron 40.0%
AGAATAGCCGTCTTCTGAAT+TGG + chr4_1:45500689-45500708 None:intergenic 40.0%
AGATAAAGATGGCCGAACTA+AGG + chr4_1:45500772-45500791 None:intergenic 40.0%
TAGTTCGGCCATCTTTATCT+TGG - chr4_1:45500772-45500791 Msa0550080:five_prime_UTR 40.0%
TCTTTGGGCCAAGATAAAGA+TGG + chr4_1:45500783-45500802 None:intergenic 40.0%
TATTCCCCTACTTATTGAGG+TGG - chr4_1:45500947-45500966 Msa0550080:five_prime_UTR 40.0%
! AATCATGGTGGAGAGACTAA+TGG + chr4_1:45501026-45501045 None:intergenic 40.0%
GTGGTAAGAGAAGATGTGGA+TGG + chr4_1:45498144-45498163 None:intergenic 45.0%
TGAGGTGGTAAGAGAAGATG+TGG + chr4_1:45498148-45498167 None:intergenic 45.0%
AAAGTAGAACTGTCTCGCGA+TGG - chr4_1:45499053-45499072 Msa0550080:intron 45.0%
CGCGATGGAGAATTCGTATA+AGG - chr4_1:45499068-45499087 Msa0550080:intron 45.0%
GGTAACGAACCCTTTATCGA+GGG + chr4_1:45499156-45499175 None:intergenic 45.0%
CGTTACCGTGGTTAGACATA+AGG - chr4_1:45499169-45499188 Msa0550080:intron 45.0%
AAGTGTGAGAATCTCAGGAG+TGG + chr4_1:45499235-45499254 None:intergenic 45.0%
GGATTCCAACCCTTCTCAAA+AGG - chr4_1:45499325-45499344 Msa0550080:CDS 45.0%
!! CTATCCCTTTTGAGAAGGGT+TGG + chr4_1:45499333-45499352 None:intergenic 45.0%
CAACCCTTCTCAAAAGGGAT+AGG - chr4_1:45499331-45499350 Msa0550080:CDS 45.0%
!! CACCCTATCCCTTTTGAGAA+GGG + chr4_1:45499337-45499356 None:intergenic 45.0%
! GGAGAAGCAATTGTTGTTGC+CGG - chr4_1:45499572-45499591 Msa0550080:CDS 45.0%
! TCTGGAATTGCATTCAACGC+CGG + chr4_1:45499708-45499727 None:intergenic 45.0%
GACTCCGTGTTGTCAAATTG+CGG - chr4_1:45499886-45499905 Msa0550080:CDS 45.0%
AGTGCCGCAATTTGACAACA+CGG + chr4_1:45499893-45499912 None:intergenic 45.0%
ACTATAGCGCTATGCCCTTT+AGG - chr4_1:45499910-45499929 Msa0550080:CDS 45.0%
CTATAGCGCTATGCCCTTTA+GGG - chr4_1:45499911-45499930 Msa0550080:CDS 45.0%
GACTGATGTCACAACCTAGT+AGG - chr4_1:45500154-45500173 Msa0550080:intron 45.0%
TGTCACAACCTAGTAGGAGA+TGG - chr4_1:45500160-45500179 Msa0550080:intron 45.0%
ACAAGAACAGCATACGCTGA+CGG - chr4_1:45500537-45500556 Msa0550080:intron 45.0%
! ATGGTTTAACGCCGGTGTTA+GGG - chr4_1:45500578-45500597 Msa0550080:intron 45.0%
!! GTTAGGGTTGGTGTTGGTAT+TGG - chr4_1:45500594-45500613 Msa0550080:intron 45.0%
GTTTGTCTTGGAATCGGCAT+TGG - chr4_1:45500624-45500643 Msa0550080:intron 45.0%
! AAATCATACCAAGGTGCCAC+CGG - chr4_1:45500663-45500682 Msa0550080:intron 45.0%
! GTCTTGTTCATCGAGTGCAA+AGG - chr4_1:45500815-45500834 Msa0550080:five_prime_UTR 45.0%
TTGCATGTGTCGTGTTTCGC+CGG - chr4_1:45498438-45498457 Msa0550080:CDS 50.0%
CGGTAACGAACCCTTTATCG+AGG + chr4_1:45499157-45499176 None:intergenic 50.0%
CGATAAAGGGTTCGTTACCG+TGG - chr4_1:45499157-45499176 Msa0550080:intron 50.0%
!! CCACCCTATCCCTTTTGAGA+AGG + chr4_1:45499338-45499357 None:intergenic 50.0%
CCTTCTCAAAAGGGATAGGG+TGG - chr4_1:45499335-45499354 Msa0550080:CDS 50.0%
! GGGTGGTAGTGTGAAGTTTG+AGG - chr4_1:45499407-45499426 Msa0550080:CDS 50.0%
TATATTGCCGGGTCCTTCTC+TGG - chr4_1:45499621-45499640 Msa0550080:CDS 50.0%
TGACAACACGGAGTCACACA+TGG + chr4_1:45499881-45499900 None:intergenic 50.0%
GCGAAGATGGGGAACTTTCA+TGG - chr4_1:45500559-45500578 Msa0550080:intron 50.0%
CATGGTTTAACGCCGGTGTT+AGG - chr4_1:45500577-45500596 Msa0550080:intron 50.0%
! TTTAACGCCGGTGTTAGGGT+TGG - chr4_1:45500582-45500601 Msa0550080:intron 50.0%
ACCAACACCAACCCTAACAC+CGG + chr4_1:45500592-45500611 None:intergenic 50.0%
! CTTGGAATCGGCATTGGAGT+TGG - chr4_1:45500630-45500649 Msa0550080:intron 50.0%
TAGCCGTCTTCTGAATTGGC+CGG + chr4_1:45500685-45500704 None:intergenic 50.0%
! GATGATCGGAGTGCCAGAGA+AGG + chr4_1:45499637-45499656 None:intergenic 55.0%
TCCTTCTGCGCTTGCTTTGC+AGG - chr4_1:45499755-45499774 Msa0550080:CDS 55.0%
ACCTGCAAAGCAAGCGCAGA+AGG + chr4_1:45499759-45499778 None:intergenic 55.0%
CATACGCTGACGGCGAAGAT+GGG - chr4_1:45500547-45500566 Msa0550080:intron 55.0%
ATACGCTGACGGCGAAGATG+GGG - chr4_1:45500548-45500567 Msa0550080:intron 55.0%
CCACCGGCCAATTCAGAAGA+CGG - chr4_1:45500679-45500698 Msa0550080:intron 55.0%
GCATACGCTGACGGCGAAGA+TGG - chr4_1:45500546-45500565 Msa0550080:intron 60.0%
!! GCCGGTGTTAGGGTTGGTGT+TGG - chr4_1:45500588-45500607 Msa0550080:intron 60.0%
TGAATTGGCCGGTGGCACCT+TGG + chr4_1:45500674-45500693 None:intergenic 60.0%
CCGTCTTCTGAATTGGCCGG+TGG + chr4_1:45500682-45500701 None:intergenic 60.0%
! CGGAGTGCCAGAGAAGGACC+CGG + chr4_1:45499631-45499650 None:intergenic 65.0%
TGCATGTGCTCTCGAGCCGG+AGG + chr4_1:45500298-45500317 None:intergenic 65.0%
! TCCGGCTCGAGAGCACATGC+AGG - chr4_1:45500297-45500316 Msa0550080:intron 65.0%
GCCTGCATGTGCTCTCGAGC+CGG + chr4_1:45500301-45500320 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 45497918 45501124 45497918 ID=Msa0550080;Name=Msa0550080
chr4_1 mRNA 45497918 45501124 45497918 ID=Msa0550080-mRNA-1;Parent=Msa0550080;Name=Msa0550080-mRNA-1;_AED=0.17;_eAED=0.17;_QI=515|1|1|1|0.5|0.33|3|414|310
chr4_1 exon 45497918 45498559 45497918 ID=Msa0550080-mRNA-1:exon:12029;Parent=Msa0550080-mRNA-1
chr4_1 exon 45499266 45500060 45499266 ID=Msa0550080-mRNA-1:exon:12028;Parent=Msa0550080-mRNA-1
chr4_1 exon 45500700 45501124 45500700 ID=Msa0550080-mRNA-1:exon:12027;Parent=Msa0550080-mRNA-1
chr4_1 five_prime_UTR 45500700 45501124 45500700 ID=Msa0550080-mRNA-1:five_prime_utr;Parent=Msa0550080-mRNA-1
chr4_1 five_prime_UTR 45499971 45500060 45499971 ID=Msa0550080-mRNA-1:five_prime_utr;Parent=Msa0550080-mRNA-1
chr4_1 CDS 45499266 45499970 45499266 ID=Msa0550080-mRNA-1:cds;Parent=Msa0550080-mRNA-1
chr4_1 CDS 45498332 45498559 45498332 ID=Msa0550080-mRNA-1:cds;Parent=Msa0550080-mRNA-1
chr4_1 three_prime_UTR 45497918 45498331 45497918 ID=Msa0550080-mRNA-1:three_prime_utr;Parent=Msa0550080-mRNA-1
Gene Sequence

>Msa0550080

ATGGAGAATTCGTATAAGGTTAATGGAAATGGACCAAGTGAAAATGGATACTCTATTGCTAGGCACACGCACTCATATCAACCCTCGATAAAGGGTTCGTTACCGTGGTTAGACATAAGGGTGTTTTATGTAAGGGTTTGCAAGTGTGAGCTTGATAATTCCACTCCTGAGATTCTCACACTTAATCATGTTCCTTTAAATCCTGATACCCTTCTTGAAGTTAATGGTGTTCGGAGTAGTTTATATTCTGATGGGATTCCAACCCTTCTCAAAAGGGATAGGGTGGATAGAAAATCGGAAGAAGTTACGTTTGTAAGCACTGATAGTATAAGGATGGGTGGTAGTGTGAAGTTTGAGGTGTTTGATAAAGATAAGCTTCTTCTGTTTGGTACCTTAGAATTATGTAACAGCAATGGTGTTGTTAGAGAATCAAATTCTAATGGACAAAGTTGGAGCATGAAGTGTGAGTCGAATATAATTCCAGGCACAAAGTTCTTTAAGGAGAAGCAATTGTTGTTGCCGGACTCTACTCTTCCAACAATTGAGGTCTATATTGCCGGGTCCTTCTCTGGCACTCCGATCATCTTAACGAAGACCTTGCATCTTAGTTCTCAAAAAAGACATACAAGGAAGCCGGCGTTGAATGCAATTCCAGAGAATGATGCAAATGAAAATGGGAAAGGTCCTTCTGCGCTTGCTTTGCAGGCTCCAGATTACATGTATGACAAACATGATGATGAGGATTATCACGGCCTATACACAAGAACAGCATACGCTGACGGCGAAGATGGGGAACTTTCATGGTTTAACGCCGGTGTTAGGGTTGGTGTTGGTATTGGACTTAGTGTTTGTCTTGGAATCGGCATTGGAGTTGGCATACTCGTTAAATCATACCAAGGTGCCACCGGCCAATTCAGAAGACGGCTATTCTAA
Protein sequence

>Msa0550080

MENSYKVNGNGPSENGYSIARHTHSYQPSIKGSLPWLDIRVFYVRVCKCELDNSTPEILTLNHVPLNPDTLLEVNGVRSSLYSDGIPTLLKRDRVDRKSEEVTFVSTDSIRMGGSVKFEVFDKDKLLLFGTLELCNSNGVVRESNSNGQSWSMKCESNIIPGTKFFKEKQLLLPDSTLPTIEVYIAGSFSGTPIILTKTLHLSSQKRHTRKPALNAIPENDANENGKGPSALALQAPDYMYDKHDDEDYHGLYTRTAYADGEDGELSWFNAGVRVGVGIGLSVCLGIGIGVGILVKSYQGATGQFRRRLF*