AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0607350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0607350 MtrunA17_Chr4g0038941 95.268 317 15 0 15 331 42 358 0.0 615
Msa0607350 MtrunA17_Chr4g0039011 67.622 349 95 3 1 331 1 349 1.51e-168 473
Msa0607350 MtrunA17_Chr4g0038961 70.181 332 95 3 1 331 1 329 9.69e-167 467
Msa0607350 MtrunA17_Chr4g0038971 68.373 332 101 3 1 331 1 329 2.40e-166 466
Msa0607350 MtrunA17_Chr4g0038981 69.578 332 97 3 1 331 1 329 2.61e-165 463
Msa0607350 MtrunA17_Chr4g0039071 58.147 313 124 4 23 328 23 335 6.30e-126 365
Msa0607350 MtrunA17_Chr1g0174261 61.053 285 100 5 54 329 1 283 7.61e-116 338
Msa0607350 MtrunA17_Chr4g0039021 58.703 293 115 5 45 331 1 293 1.38e-113 331
Msa0607350 MtrunA17_Chr2g0294591 53.870 323 137 8 17 330 23 342 8.05e-110 324
Msa0607350 MtrunA17_Chr2g0294601 52.836 335 145 7 5 330 41 371 2.41e-109 323
Msa0607350 MtrunA17_Chr7g0250301 43.396 318 169 8 19 328 2 316 3.59e-82 252
Msa0607350 MtrunA17_Chr5g0439251 41.486 323 173 8 24 331 24 345 2.10e-75 239
Msa0607350 MtrunA17_Chr3g0119541 42.462 325 167 8 24 331 25 346 2.78e-75 236
Msa0607350 MtrunA17_Chr8g0389741 38.199 322 182 7 25 331 28 347 3.55e-75 239
Msa0607350 MtrunA17_Chr1g0189111 39.815 324 183 6 20 331 26 349 3.68e-72 229
Msa0607350 MtrunA17_Chr6g0487261 35.277 343 200 7 2 331 13 346 3.21e-71 229
Msa0607350 MtrunA17_Chr4g0026321 38.871 319 180 6 24 328 28 345 1.25e-70 224
Msa0607350 MtrunA17_Chr4g0043851 38.372 344 199 6 1 331 8 351 4.10e-70 223
Msa0607350 MtrunA17_Chr1g0177171 39.319 323 180 8 24 331 37 358 8.19e-70 225
Msa0607350 MtrunA17_Chr1g0146831 39.403 335 182 9 8 327 16 344 8.47e-70 222
Msa0607350 MtrunA17_Chr4g0034901 39.053 338 191 8 6 328 9 346 1.90e-69 223
Msa0607350 MtrunA17_Chr4g0003281 37.318 343 201 6 3 331 18 360 4.97e-69 221
Msa0607350 MtrunA17_Chr4g0046311 36.890 328 186 8 24 331 22 348 2.72e-68 221
Msa0607350 MtrunA17_Chr3g0144531 38.192 343 193 9 6 331 8 348 9.10e-68 219
Msa0607350 MtrunA17_Chr2g0321511 35.882 340 195 9 3 328 13 343 1.61e-67 216
Msa0607350 MtrunA17_Chr1g0161011 38.218 348 196 8 1 331 1 346 1.60e-66 217
Msa0607350 MtrunA17_Chr4g0064021 37.072 321 188 7 24 330 25 345 8.80e-66 212
Msa0607350 MtrunA17_Chr3g0110371 38.080 323 184 6 25 331 24 346 3.47e-65 213
Msa0607350 MtrunA17_Chr2g0285901 38.906 329 183 8 17 328 12 339 4.78e-64 207
Msa0607350 MtrunA17_Chr7g0223961 39.252 321 176 11 20 326 18 333 1.32e-63 208
Msa0607350 MtrunA17_Chr7g0250321 41.901 284 154 7 2 279 8 286 6.93e-63 201
Msa0607350 MtrunA17_Chr7g0234821 36.111 324 188 8 26 331 27 349 3.00e-62 205
Msa0607350 MtrunA17_Chr8g0339191 35.714 350 198 11 3 331 9 352 2.07e-61 202
Msa0607350 MtrunA17_Chr8g0383311 37.920 327 183 10 22 331 40 363 2.13e-61 203
Msa0607350 MtrunA17_Chr1g0155001 36.728 324 186 8 25 331 24 345 3.28e-61 201
Msa0607350 MtrunA17_Chr5g0434591 35.530 349 201 9 1 331 3 345 1.10e-60 201
Msa0607350 MtrunA17_Chr3g0138741 37.758 339 187 13 11 330 17 350 1.71e-58 196
Msa0607350 MtrunA17_Chr7g0253091 35.632 348 195 12 4 331 9 347 1.87e-57 192
Msa0607350 MtrunA17_Chr8g0378621 34.104 346 207 8 1 330 7 347 1.38e-55 188
Msa0607350 MtrunA17_Chr3g0129341 37.536 349 188 15 3 331 14 352 1.41e-55 188
Msa0607350 MtrunA17_Chr1g0148391 36.919 344 192 14 4 329 8 344 1.30e-54 185
Msa0607350 MtrunA17_Chr2g0290821 37.003 327 182 11 24 330 27 349 1.64e-54 185
Msa0607350 MtrunA17_Chr3g0121271 33.642 324 198 7 25 331 26 349 4.05e-54 184
Msa0607350 MtrunA17_Chr4g0062771 33.239 355 197 13 1 329 11 351 1.89e-53 182
Msa0607350 MtrunA17_Chr4g0075031 34.154 325 194 7 25 331 45 367 1.59e-52 181
Msa0607350 MtrunA17_Chr6g0461601 34.006 347 201 11 6 331 10 349 3.45e-52 179
Msa0607350 MtrunA17_Chr4g0043511 37.304 319 182 10 25 328 62 377 5.32e-52 180
Msa0607350 MtrunA17_Chr2g0306311 32.979 376 191 16 2 329 14 376 3.41e-50 174
Msa0607350 MtrunA17_Chr4g0069801 35.065 308 180 10 40 330 43 347 7.47e-50 173
Msa0607350 MtrunA17_Chr4g0076581 35.583 326 186 11 24 330 32 352 1.66e-49 172
Msa0607350 MtrunA17_Chr3g0105981 32.931 331 190 12 20 328 22 342 1.03e-47 166
Msa0607350 MtrunA17_Chr4g0018861 68.182 88 28 0 242 329 87 174 1.82e-36 130
Msa0607350 MtrunA17_Chr4g0018861 46.667 90 45 1 1 90 1 87 6.32e-21 89.4
Msa0607350 MtrunA17_Chr5g0436361 32.776 299 176 13 51 331 63 354 4.53e-36 134
Msa0607350 MtrunA17_Chr2g0294581 44.086 186 87 8 14 191 66 242 2.12e-35 130
Msa0607350 MtrunA17_Chr2g0294581 53.000 100 43 4 233 330 187 284 9.70e-26 103
Msa0607350 MtrunA17_Chr4g0018771 62.637 91 34 0 239 329 86 176 7.42e-35 126
Msa0607350 MtrunA17_Chr4g0018771 56.338 71 31 0 23 93 24 94 1.83e-20 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0607350 AT4G16260.1 52.663 338 150 6 1 331 1 335 3.53e-117 342
Msa0607350 AT3G57270.1 53.035 313 140 4 25 330 27 339 6.77e-112 328
Msa0607350 AT4G16260.2 53.595 306 133 5 33 331 3 306 1.03e-108 320
Msa0607350 AT3G57240.1 51.411 319 141 5 20 331 30 341 2.53e-108 320
Msa0607350 AT3G57260.1 49.702 336 152 8 1 330 14 338 3.54e-102 304
Msa0607350 AT3G57260.2 49.702 336 152 8 1 330 30 354 5.38e-102 304
Msa0607350 AT2G05790.1 41.615 322 174 7 24 331 24 345 3.08e-75 239
Msa0607350 AT4G26830.1 40.938 320 176 6 25 331 25 344 4.59e-74 236
Msa0607350 AT4G26830.2 40.938 320 176 6 25 331 29 348 6.11e-74 235
Msa0607350 AT5G56590.1 37.267 322 187 6 25 331 26 347 9.27e-74 236
Msa0607350 AT2G01630.2 41.176 323 174 6 25 331 23 345 9.38e-74 233
Msa0607350 AT2G01630.3 41.176 323 174 6 25 331 23 345 4.56e-73 233
Msa0607350 AT3G15800.1 38.576 337 190 7 10 331 30 364 6.01e-73 231
Msa0607350 AT2G01630.1 41.176 323 174 6 25 331 23 345 4.31e-72 232
Msa0607350 AT5G42100.2 41.358 324 174 7 21 328 23 346 5.02e-72 229
Msa0607350 AT5G55180.2 39.193 347 195 8 1 331 1 347 5.27e-72 231
Msa0607350 AT5G42100.1 41.358 324 174 7 21 328 23 346 9.55e-72 229
Msa0607350 AT5G55180.1 39.193 347 195 8 1 331 1 347 1.00e-71 229
Msa0607350 AT5G20330.1 40.312 320 176 10 20 328 29 344 4.74e-71 224
Msa0607350 AT4G18340.2 37.830 341 197 7 4 331 15 353 7.28e-71 225
Msa0607350 AT4G18340.1 37.830 341 197 7 4 331 15 353 8.72e-71 225
Msa0607350 AT1G77780.1 40.446 314 170 9 26 328 25 332 1.69e-70 224
Msa0607350 AT2G26600.1 35.629 334 197 7 15 330 20 353 1.28e-69 222
Msa0607350 AT2G16230.1 39.875 321 179 6 25 331 25 345 1.73e-69 225
Msa0607350 AT1G77790.1 37.168 339 197 9 1 328 8 341 2.19e-69 220
Msa0607350 AT4G29360.2 38.261 345 198 7 1 331 13 356 3.44e-69 225
Msa0607350 AT5G20340.1 41.456 316 170 10 24 328 42 353 3.97e-69 220
Msa0607350 AT1G30080.2 36.765 340 202 5 2 328 13 352 9.66e-68 217
Msa0607350 AT1G30080.1 36.765 340 202 5 2 328 13 352 1.09e-67 218
Msa0607350 AT4G29360.1 38.123 341 195 8 6 331 7 346 1.92e-67 221
Msa0607350 AT4G34480.2 38.776 343 191 7 3 331 9 346 2.04e-67 215
Msa0607350 AT1G33220.1 41.176 306 166 9 24 318 33 335 2.88e-67 214
Msa0607350 AT5G42720.1 38.150 346 193 9 2 331 7 347 2.28e-66 215
Msa0607350 AT5G20390.1 38.393 336 191 11 5 328 12 343 2.61e-66 212
Msa0607350 AT5G24318.4 38.596 342 184 11 4 328 12 344 5.60e-66 212
Msa0607350 AT1G32860.1 36.842 323 189 6 24 331 28 350 5.75e-66 214
Msa0607350 AT4G34480.1 38.776 343 191 7 3 331 9 346 1.09e-65 215
Msa0607350 AT2G27500.2 34.688 320 195 7 23 328 27 346 2.37e-65 211
Msa0607350 AT2G27500.1 34.688 320 195 7 23 328 27 346 2.77e-65 211
Msa0607350 AT5G24318.2 38.596 342 184 11 4 328 12 344 1.39e-64 211
Msa0607350 AT3G07320.1 36.420 324 187 8 26 331 26 348 2.08e-64 211
Msa0607350 AT5G24318.1 38.596 342 184 11 4 328 12 344 2.51e-64 210
Msa0607350 AT1G66250.1 39.009 323 180 7 25 331 32 353 2.92e-63 209
Msa0607350 AT5G20560.1 36.747 332 189 9 7 320 8 336 1.59e-62 202
Msa0607350 AT4G14080.1 35.952 331 188 10 21 331 38 364 1.70e-62 206
Msa0607350 AT3G61810.1 35.294 323 188 6 25 331 55 372 1.01e-60 199
Msa0607350 AT3G23770.1 35.952 331 191 8 21 331 33 362 2.23e-60 201
Msa0607350 AT3G13560.4 34.591 318 192 6 25 326 26 343 1.75e-55 188
Msa0607350 AT3G13560.1 34.591 318 192 6 25 326 26 343 1.75e-55 188
Msa0607350 AT3G13560.2 34.591 318 192 6 25 326 26 343 1.75e-55 188
Msa0607350 AT3G13560.5 34.591 318 192 6 25 326 26 343 1.75e-55 188
Msa0607350 AT3G13560.3 34.591 318 192 6 25 326 26 343 1.75e-55 188
Msa0607350 AT1G11820.1 32.923 325 198 7 25 331 44 366 2.65e-55 185
Msa0607350 AT5G58090.1 36.923 325 183 12 24 330 21 341 3.70e-55 187
Msa0607350 AT2G19440.2 35.277 343 200 12 7 331 3 341 5.82e-55 186
Msa0607350 AT2G19440.1 35.277 343 200 12 7 331 3 341 5.82e-55 186
Msa0607350 AT5G18220.1 35.168 327 190 11 22 331 27 348 3.88e-54 184
Msa0607350 AT1G11820.3 32.923 325 198 7 25 331 44 366 5.04e-54 184
Msa0607350 AT3G24330.1 34.682 346 206 10 4 331 18 361 6.94e-54 184
Msa0607350 AT4G31140.1 35.652 345 197 13 4 330 9 346 7.87e-54 184
Msa0607350 AT1G11820.2 32.923 325 198 7 25 331 44 366 1.13e-53 184
Msa0607350 AT5G58480.1 34.441 331 199 8 16 330 17 345 3.01e-53 182
Msa0607350 AT3G46570.1 36.686 338 186 12 11 328 13 342 3.50e-53 179
Msa0607350 AT2G27500.3 35.075 268 160 7 75 328 1 268 3.60e-53 177
Msa0607350 AT1G64760.1 35.061 328 193 10 21 331 21 345 4.24e-53 182
Msa0607350 AT1G64760.2 35.061 328 193 10 21 331 21 345 4.24e-53 182
Msa0607350 AT2G26600.2 35.798 257 152 5 87 330 3 259 1.14e-52 176
Msa0607350 AT3G04010.1 33.528 343 204 11 7 330 15 352 4.19e-52 179
Msa0607350 AT5G64790.1 33.621 348 205 12 4 331 7 348 1.50e-51 177
Msa0607350 AT3G55430.1 35.604 323 187 11 21 328 25 341 3.97e-51 176
Msa0607350 AT2G39640.1 32.941 340 205 11 1 328 10 338 1.48e-50 176
Msa0607350 AT4G34480.3 42.060 233 123 4 111 331 3 235 1.16e-48 168
Msa0607350 AT4G17180.1 32.653 343 206 11 3 330 7 339 6.90e-48 167
Msa0607350 AT5G20870.1 31.534 352 212 12 3 330 10 356 1.32e-43 157
Msa0607350 AT5G24318.3 40.000 210 109 6 131 328 1 205 3.02e-40 144
Msa0607350 AT3G55780.1 30.464 302 187 11 48 331 58 354 9.26e-36 133

Find 88 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAACTAATTTGATATTT+AGG 0.142764 4_2:-54152212 None:intergenic
TCTATCATATTGCTGCTTAT+TGG 0.152916 4_2:+54150884 Msa0607350:CDS
GGAAATGCACAAACATATTA+TGG 0.170246 4_2:+54152044 Msa0607350:CDS
CTTCTATGTTTGAACCTCTA+AGG 0.203767 4_2:-54151440 None:intergenic
GATGCACGTACCTTACTTAT+TGG 0.215185 4_2:-54152318 None:intergenic
TCGCGGTAGACACTTTAATT+TGG 0.242868 4_2:-54151677 None:intergenic
GCGATAGACACAACACTAAT+TGG 0.256481 4_2:+54151696 Msa0607350:CDS
GACCCAATCGTTCGCGGTTT+GGG 0.260463 4_2:-54151508 None:intergenic
TTATTAACATATGAGAAATA+AGG 0.277410 4_2:-54151831 None:intergenic
TTCCACTGCATTAGAATTTA+CGG 0.279344 4_2:+54150919 Msa0607350:CDS
CAATCCGTCGGTGTTTGTTA+TGG 0.312388 4_2:+54151309 Msa0607350:CDS
ACATTGCATCAAACAAGTTT+TGG 0.313565 4_2:-54151917 None:intergenic
TTATGCTTTGTTTACAAAAC+AGG 0.317873 4_2:+54151875 Msa0607350:CDS
TCAACCACTGCTTGCTTTGT+TGG 0.330339 4_2:-54151351 None:intergenic
ATAGGTCCATTAGGCCTCTT+AGG 0.339039 4_2:-54152098 None:intergenic
GAATTTGATTAAACATGTTA+AGG 0.340941 4_2:+54152067 Msa0607350:CDS
TGACCCAATCGTTCGCGGTT+TGG 0.343008 4_2:-54151509 None:intergenic
GTACGTGCATCGTGCTCTTA+TGG 0.350194 4_2:+54152330 Msa0607350:three_prime_UTR
AATCAAGACCAATGCTTTGT+TGG 0.361398 4_2:-54151854 None:intergenic
TCCACTGCATTAGAATTTAC+GGG 0.389494 4_2:+54150920 Msa0607350:CDS
ATGCCCAAACCGCGAACGAT+TGG 0.395703 4_2:+54151505 Msa0607350:CDS
GTTGATTTATACAAATCAAA+AGG 0.397564 4_2:+54151369 Msa0607350:CDS
GGTATACTCCAAGCCCTTAG+AGG 0.397868 4_2:+54151426 Msa0607350:CDS
AATATATCATCATTATTCAT+TGG 0.405323 4_2:-54152374 None:intergenic
TTGATATTTAGGTGATTTGT+CGG 0.411081 4_2:-54152201 None:intergenic
GGGAAGAACTGAATTTGTTT+CGG 0.412595 4_2:-54151607 None:intergenic
AATTTGATTAAACATGTTAA+GGG 0.412973 4_2:+54152068 Msa0607350:CDS
TGGCCATCTCAAGGTGGAAC+TGG 0.422776 4_2:+54152011 Msa0607350:CDS
GCTTGGAGTATACCTTGGTC+TGG 0.424488 4_2:-54151417 None:intergenic
GGTGTTTGTTATGGAGGAAA+TGG 0.425699 4_2:+54151318 Msa0607350:CDS
GAAATGCACAAACATATTAT+GGG 0.431940 4_2:+54152045 Msa0607350:CDS
GTGATTTGAGAACGTCGTTT+GGG 0.436818 4_2:-54151476 None:intergenic
TGTTTGAACCTCTAAGGGCT+TGG 0.453566 4_2:-54151434 None:intergenic
ATGCTGCTCTTGAGAAAGTA+GGG 0.454697 4_2:+54151952 Msa0607350:CDS
TATTAACATATGAGAAATAA+GGG 0.456534 4_2:-54151830 None:intergenic
TTTGTTGACCCAATCGTTCG+CGG 0.462252 4_2:-54151514 None:intergenic
GGAACTCCTAAGAGGCCTAA+TGG 0.466080 4_2:+54152092 Msa0607350:CDS
TTCTATGTTTGAACCTCTAA+GGG 0.466463 4_2:-54151439 None:intergenic
TGCCCAAACCGCGAACGATT+GGG 0.476878 4_2:+54151506 Msa0607350:CDS
TATGCTGCTCTTGAGAAAGT+AGG 0.478615 4_2:+54151951 Msa0607350:CDS
CATAACAAACACCGACGGAT+TGG 0.479275 4_2:-54151308 None:intergenic
AAACTTGTTTGATGCAATGT+TGG 0.480831 4_2:+54151920 Msa0607350:CDS
TTATACAAATCAAAAGGGAT+TGG 0.486643 4_2:+54151375 Msa0607350:CDS
AGTGATTTGAGAACGTCGTT+TGG 0.488685 4_2:-54151477 None:intergenic
GAATTTGTTTCGGCTGAACC+CGG 0.491632 4_2:-54151597 None:intergenic
TCTGAAATTGAGCGACACTT+TGG 0.496250 4_2:+54152167 Msa0607350:CDS
CTTGAAATAGCCAATAAGTA+AGG 0.497086 4_2:+54152308 Msa0607350:three_prime_UTR
AAGATTGTTGTGTCTGAGAG+TGG 0.504963 4_2:+54151987 Msa0607350:CDS
TAAGTCTCAATAGGTCCATT+AGG 0.512608 4_2:-54152107 None:intergenic
TGATATTTAGGTGATTTGTC+GGG 0.514105 4_2:-54152200 None:intergenic
TTGATTTATACAAATCAAAA+GGG 0.517479 4_2:+54151370 Msa0607350:CDS
AAACAGGGAAAGAATGAAGT+TGG 0.526840 4_2:+54151891 Msa0607350:CDS
GTTAATAACCAACAAAGCAT+TGG 0.527284 4_2:+54151846 Msa0607350:CDS
TATGATAGACATGGTTGTGT+TGG 0.531941 4_2:-54150870 None:intergenic
AAAATCAAATACATTGCAGT+TGG 0.531962 4_2:+54151561 Msa0607350:CDS
ACGCTGCAAGTGGTTACATA+AGG 0.532172 4_2:+54151754 Msa0607350:CDS
GAGAGTGGGTGGCCATCTCA+AGG 0.533168 4_2:+54152002 Msa0607350:CDS
TTGATTAAACATGTTAAGGG+TGG 0.542157 4_2:+54152071 Msa0607350:CDS
GTTCGATGAAAATCAGAAGC+CGG 0.543224 4_2:+54152142 Msa0607350:CDS
TTCGATGAAAATCAGAAGCC+GGG 0.543831 4_2:+54152143 Msa0607350:CDS
TCAAACATAGAAGTAATCCT+TGG 0.548004 4_2:+54151450 Msa0607350:CDS
AGTTGGCAATGAAGTTCACC+CGG 0.550571 4_2:+54151578 Msa0607350:CDS
TCCGTCGGTGTTTGTTATGG+AGG 0.551421 4_2:+54151312 Msa0607350:CDS
ACCCGTAAATTCTAATGCAG+TGG 0.555138 4_2:-54150921 None:intergenic
TAAGGGCTTGGAGTATACCT+TGG 0.555442 4_2:-54151422 None:intergenic
AATTTGTTTCGGCTGAACCC+GGG 0.569052 4_2:-54151596 None:intergenic
CTTGGTCTGGATTGTATATG+CGG 0.569910 4_2:-54151404 None:intergenic
TATGCTTTGTTTACAAAACA+GGG 0.572886 4_2:+54151876 Msa0607350:CDS
GGTGGAACTGGAGCTAGTGT+TGG 0.573408 4_2:+54152023 Msa0607350:CDS
TGTCGCTCAATTTCAGAACC+CGG 0.574035 4_2:-54152161 None:intergenic
AGATTGTTGTGTCTGAGAGT+GGG 0.578374 4_2:+54151988 Msa0607350:CDS
TTAAGGGTGGAACTCCTAAG+AGG 0.585631 4_2:+54152084 Msa0607350:CDS
ATATCTTCAGCAAACTTACA+AGG 0.589493 4_2:+54151654 Msa0607350:CDS
GCTCCAGTTCCACCTTGAGA+TGG 0.597438 4_2:-54152014 None:intergenic
CATGCAGTTGTCCAATCCGT+CGG 0.599010 4_2:+54151297 Msa0607350:intron
TGCTGCTCTTGAGAAAGTAG+GGG 0.607774 4_2:+54151953 Msa0607350:CDS
GGGTAGACGTTAGCAAGAAG+TGG 0.621501 4_2:-54151810 None:intergenic
GTTGGCAATGAAGTTCACCC+GGG 0.631911 4_2:+54151579 Msa0607350:CDS
CGCATATACAATCCAGACCA+AGG 0.635364 4_2:+54151405 Msa0607350:CDS
TCCTCCATAACAAACACCGA+CGG 0.636400 4_2:-54151313 None:intergenic
AATTAGTGTTGTGTCTATCG+CGG 0.640664 4_2:-54151694 None:intergenic
AAATCTTACCCACCAAAAGA+CGG 0.644937 4_2:+54151720 Msa0607350:CDS
GCTGCTCTTGAGAAAGTAGG+GGG 0.653642 4_2:+54151954 Msa0607350:CDS
TCTACCAACAAAGCAAGCAG+TGG 0.662167 4_2:+54151347 Msa0607350:CDS
AAGCAGCAATATGATAGACA+TGG 0.667052 4_2:-54150879 None:intergenic
TTGTTGTGTCTGAGAGTGGG+TGG 0.678415 4_2:+54151991 Msa0607350:CDS
GAACGTCGTTTGGGACACCA+AGG 0.683186 4_2:-54151467 None:intergenic
AGTGGGTGGCCATCTCAAGG+TGG 0.723367 4_2:+54152005 Msa0607350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGCTAAAATAAAATAGAAAA+AGG - chr4_2:54151020-54151039 None:intergenic 15.0%
!! TACATATAACTTATTAAGTA+AGG - chr4_2:54151168-54151187 None:intergenic 15.0%
!! TTGATTTATACAAATCAAAA+GGG + chr4_2:54151370-54151389 Msa0607350:CDS 15.0%
!! TATTAACATATGAGAAATAA+GGG - chr4_2:54151833-54151852 None:intergenic 15.0%
!! TTATTAACATATGAGAAATA+AGG - chr4_2:54151834-54151853 None:intergenic 15.0%
!! AATTTGATTAAACATGTTAA+GGG + chr4_2:54152068-54152087 Msa0607350:CDS 15.0%
!! AATATATCATCATTATTCAT+TGG - chr4_2:54152377-54152396 None:intergenic 15.0%
!! TGCTAAAATAAAATAGAAAA+AGG - chr4_2:54151020-54151039 None:intergenic 15.0%
!! TACATATAACTTATTAAGTA+AGG - chr4_2:54151168-54151187 None:intergenic 15.0%
!! TTGATTTATACAAATCAAAA+GGG + chr4_2:54151370-54151389 Msa0607350:CDS 15.0%
!! TATTAACATATGAGAAATAA+GGG - chr4_2:54151833-54151852 None:intergenic 15.0%
!! TTATTAACATATGAGAAATA+AGG - chr4_2:54151834-54151853 None:intergenic 15.0%
!! AATTTGATTAAACATGTTAA+GGG + chr4_2:54152068-54152087 Msa0607350:CDS 15.0%
!! AATATATCATCATTATTCAT+TGG - chr4_2:54152377-54152396 None:intergenic 15.0%
!!! TTAATTCTTCCTTTTTTCTT+TGG + chr4_2:54151059-54151078 Msa0607350:intron 20.0%
!! ATTTATCAACCTTATATTTC+TGG + chr4_2:54151245-54151264 Msa0607350:intron 20.0%
!!! GTTGATTTATACAAATCAAA+AGG + chr4_2:54151369-54151388 Msa0607350:CDS 20.0%
!! CTAACTAAAAAGTTAACAAT+AGG - chr4_2:54151780-54151799 None:intergenic 20.0%
!! GAATTTGATTAAACATGTTA+AGG + chr4_2:54152067-54152086 Msa0607350:CDS 20.0%
!! CTGAAACTAATTTGATATTT+AGG - chr4_2:54152215-54152234 None:intergenic 20.0%
!!! TTAATTCTTCCTTTTTTCTT+TGG + chr4_2:54151059-54151078 Msa0607350:intron 20.0%
!! ATTTATCAACCTTATATTTC+TGG + chr4_2:54151245-54151264 Msa0607350:intron 20.0%
!!! GTTGATTTATACAAATCAAA+AGG + chr4_2:54151369-54151388 Msa0607350:CDS 20.0%
!! CTAACTAAAAAGTTAACAAT+AGG - chr4_2:54151780-54151799 None:intergenic 20.0%
!! GAATTTGATTAAACATGTTA+AGG + chr4_2:54152067-54152086 Msa0607350:CDS 20.0%
!! CTGAAACTAATTTGATATTT+AGG - chr4_2:54152215-54152234 None:intergenic 20.0%
! TAGAAAAAGGAGAAATAATG+AGG - chr4_2:54151007-54151026 None:intergenic 25.0%
!!! TATGAGAATAACTTGTTAAG+TGG + chr4_2:54151089-54151108 Msa0607350:intron 25.0%
!!! AACCTTATATTTCTGGTAAT+TGG + chr4_2:54151252-54151271 Msa0607350:intron 25.0%
! AACCAATTACCAGAAATATA+AGG - chr4_2:54151257-54151276 None:intergenic 25.0%
! TTATACAAATCAAAAGGGAT+TGG + chr4_2:54151375-54151394 Msa0607350:CDS 25.0%
! AAAATCAAATACATTGCAGT+TGG + chr4_2:54151561-54151580 Msa0607350:CDS 25.0%
!! TTTTTGAATGTTTTGCATTG+CGG - chr4_2:54151631-54151650 None:intergenic 25.0%
!!! GTTAACTTTTTAGTTAGCAA+CGG + chr4_2:54151783-54151802 Msa0607350:CDS 25.0%
!!! TTATGCTTTGTTTACAAAAC+AGG + chr4_2:54151875-54151894 Msa0607350:CDS 25.0%
!!! TATGCTTTGTTTACAAAACA+GGG + chr4_2:54151876-54151895 Msa0607350:CDS 25.0%
! GAAATGCACAAACATATTAT+GGG + chr4_2:54152045-54152064 Msa0607350:CDS 25.0%
!! TTGATATTTAGGTGATTTGT+CGG - chr4_2:54152204-54152223 None:intergenic 25.0%
! TAGAAAAAGGAGAAATAATG+AGG - chr4_2:54151007-54151026 None:intergenic 25.0%
!!! TATGAGAATAACTTGTTAAG+TGG + chr4_2:54151089-54151108 Msa0607350:intron 25.0%
!!! AACCTTATATTTCTGGTAAT+TGG + chr4_2:54151252-54151271 Msa0607350:intron 25.0%
! AACCAATTACCAGAAATATA+AGG - chr4_2:54151257-54151276 None:intergenic 25.0%
! TTATACAAATCAAAAGGGAT+TGG + chr4_2:54151375-54151394 Msa0607350:CDS 25.0%
! AAAATCAAATACATTGCAGT+TGG + chr4_2:54151561-54151580 Msa0607350:CDS 25.0%
!! TTTTTGAATGTTTTGCATTG+CGG - chr4_2:54151631-54151650 None:intergenic 25.0%
!!! GTTAACTTTTTAGTTAGCAA+CGG + chr4_2:54151783-54151802 Msa0607350:CDS 25.0%
!!! TTATGCTTTGTTTACAAAAC+AGG + chr4_2:54151875-54151894 Msa0607350:CDS 25.0%
!!! TATGCTTTGTTTACAAAACA+GGG + chr4_2:54151876-54151895 Msa0607350:CDS 25.0%
! GAAATGCACAAACATATTAT+GGG + chr4_2:54152045-54152064 Msa0607350:CDS 25.0%
!! TTGATATTTAGGTGATTTGT+CGG - chr4_2:54152204-54152223 None:intergenic 25.0%
! TCTATCATATTGCTGCTTAT+TGG + chr4_2:54150884-54150903 Msa0607350:CDS 30.0%
TTCCACTGCATTAGAATTTA+CGG + chr4_2:54150919-54150938 Msa0607350:CDS 30.0%
TAGTCATAGCCAAAGAAAAA+AGG - chr4_2:54151071-54151090 None:intergenic 30.0%
TTCTATGTTTGAACCTCTAA+GGG - chr4_2:54151442-54151461 None:intergenic 30.0%
TCAAACATAGAAGTAATCCT+TGG + chr4_2:54151450-54151469 Msa0607350:CDS 30.0%
! TTTTGAATGTTTTGCATTGC+GGG - chr4_2:54151630-54151649 None:intergenic 30.0%
ATATCTTCAGCAAACTTACA+AGG + chr4_2:54151654-54151673 Msa0607350:CDS 30.0%
GTTAATAACCAACAAAGCAT+TGG + chr4_2:54151846-54151865 Msa0607350:CDS 30.0%
! ACATTGCATCAAACAAGTTT+TGG - chr4_2:54151920-54151939 None:intergenic 30.0%
! AAACTTGTTTGATGCAATGT+TGG + chr4_2:54151920-54151939 Msa0607350:CDS 30.0%
GGAAATGCACAAACATATTA+TGG + chr4_2:54152044-54152063 Msa0607350:CDS 30.0%
TTGATTAAACATGTTAAGGG+TGG + chr4_2:54152071-54152090 Msa0607350:CDS 30.0%
GCAAAAAGATAAGTCTCAAT+AGG - chr4_2:54152119-54152138 None:intergenic 30.0%
! TGATATTTAGGTGATTTGTC+GGG - chr4_2:54152203-54152222 None:intergenic 30.0%
CTTGAAATAGCCAATAAGTA+AGG + chr4_2:54152308-54152327 Msa0607350:three_prime_UTR 30.0%
! TCTATCATATTGCTGCTTAT+TGG + chr4_2:54150884-54150903 Msa0607350:CDS 30.0%
TTCCACTGCATTAGAATTTA+CGG + chr4_2:54150919-54150938 Msa0607350:CDS 30.0%
TAGTCATAGCCAAAGAAAAA+AGG - chr4_2:54151071-54151090 None:intergenic 30.0%
TTCTATGTTTGAACCTCTAA+GGG - chr4_2:54151442-54151461 None:intergenic 30.0%
TCAAACATAGAAGTAATCCT+TGG + chr4_2:54151450-54151469 Msa0607350:CDS 30.0%
! TTTTGAATGTTTTGCATTGC+GGG - chr4_2:54151630-54151649 None:intergenic 30.0%
ATATCTTCAGCAAACTTACA+AGG + chr4_2:54151654-54151673 Msa0607350:CDS 30.0%
GTTAATAACCAACAAAGCAT+TGG + chr4_2:54151846-54151865 Msa0607350:CDS 30.0%
! ACATTGCATCAAACAAGTTT+TGG - chr4_2:54151920-54151939 None:intergenic 30.0%
! AAACTTGTTTGATGCAATGT+TGG + chr4_2:54151920-54151939 Msa0607350:CDS 30.0%
GGAAATGCACAAACATATTA+TGG + chr4_2:54152044-54152063 Msa0607350:CDS 30.0%
TTGATTAAACATGTTAAGGG+TGG + chr4_2:54152071-54152090 Msa0607350:CDS 30.0%
GCAAAAAGATAAGTCTCAAT+AGG - chr4_2:54152119-54152138 None:intergenic 30.0%
! TGATATTTAGGTGATTTGTC+GGG - chr4_2:54152203-54152222 None:intergenic 30.0%
CTTGAAATAGCCAATAAGTA+AGG + chr4_2:54152308-54152327 Msa0607350:three_prime_UTR 30.0%
TATGATAGACATGGTTGTGT+TGG - chr4_2:54150873-54150892 None:intergenic 35.0%
AAGCAGCAATATGATAGACA+TGG - chr4_2:54150882-54150901 None:intergenic 35.0%
TCCACTGCATTAGAATTTAC+GGG + chr4_2:54150920-54150939 Msa0607350:CDS 35.0%
CTTCTATGTTTGAACCTCTA+AGG - chr4_2:54151443-54151462 None:intergenic 35.0%
! GGGAAGAACTGAATTTGTTT+CGG - chr4_2:54151610-54151629 None:intergenic 35.0%
AATTAGTGTTGTGTCTATCG+CGG - chr4_2:54151697-54151716 None:intergenic 35.0%
AAATCTTACCCACCAAAAGA+CGG + chr4_2:54151720-54151739 Msa0607350:CDS 35.0%
! TAAAAACACCGTCTTTTGGT+GGG - chr4_2:54151731-54151750 None:intergenic 35.0%
AATCAAGACCAATGCTTTGT+TGG - chr4_2:54151857-54151876 None:intergenic 35.0%
AAACAGGGAAAGAATGAAGT+TGG + chr4_2:54151891-54151910 Msa0607350:CDS 35.0%
TAAGTCTCAATAGGTCCATT+AGG - chr4_2:54152110-54152129 None:intergenic 35.0%
TATGATAGACATGGTTGTGT+TGG - chr4_2:54150873-54150892 None:intergenic 35.0%
AAGCAGCAATATGATAGACA+TGG - chr4_2:54150882-54150901 None:intergenic 35.0%
TCCACTGCATTAGAATTTAC+GGG + chr4_2:54150920-54150939 Msa0607350:CDS 35.0%
CTTCTATGTTTGAACCTCTA+AGG - chr4_2:54151443-54151462 None:intergenic 35.0%
! GGGAAGAACTGAATTTGTTT+CGG - chr4_2:54151610-54151629 None:intergenic 35.0%
AATTAGTGTTGTGTCTATCG+CGG - chr4_2:54151697-54151716 None:intergenic 35.0%
AAATCTTACCCACCAAAAGA+CGG + chr4_2:54151720-54151739 Msa0607350:CDS 35.0%
! TAAAAACACCGTCTTTTGGT+GGG - chr4_2:54151731-54151750 None:intergenic 35.0%
AATCAAGACCAATGCTTTGT+TGG - chr4_2:54151857-54151876 None:intergenic 35.0%
AAACAGGGAAAGAATGAAGT+TGG + chr4_2:54151891-54151910 Msa0607350:CDS 35.0%
TAAGTCTCAATAGGTCCATT+AGG - chr4_2:54152110-54152129 None:intergenic 35.0%
ACCCGTAAATTCTAATGCAG+TGG - chr4_2:54150924-54150943 None:intergenic 40.0%
TCCTCTCTCTCTAATTAGCA+TGG + chr4_2:54150953-54150972 Msa0607350:intron 40.0%
TCCATGCTAATTAGAGAGAG+AGG - chr4_2:54150957-54150976 None:intergenic 40.0%
!! GGTGTTTGTTATGGAGGAAA+TGG + chr4_2:54151318-54151337 Msa0607350:CDS 40.0%
CTTGGTCTGGATTGTATATG+CGG - chr4_2:54151407-54151426 None:intergenic 40.0%
GTGATTTGAGAACGTCGTTT+GGG - chr4_2:54151479-54151498 None:intergenic 40.0%
AGTGATTTGAGAACGTCGTT+TGG - chr4_2:54151480-54151499 None:intergenic 40.0%
TCGCGGTAGACACTTTAATT+TGG - chr4_2:54151680-54151699 None:intergenic 40.0%
GCGATAGACACAACACTAAT+TGG + chr4_2:54151696-54151715 Msa0607350:CDS 40.0%
! CTAAAAACACCGTCTTTTGG+TGG - chr4_2:54151732-54151751 None:intergenic 40.0%
! TCGCTAAAAACACCGTCTTT+TGG - chr4_2:54151735-54151754 None:intergenic 40.0%
TATGCTGCTCTTGAGAAAGT+AGG + chr4_2:54151951-54151970 Msa0607350:CDS 40.0%
ATGCTGCTCTTGAGAAAGTA+GGG + chr4_2:54151952-54151971 Msa0607350:CDS 40.0%
AAGATTGTTGTGTCTGAGAG+TGG + chr4_2:54151987-54152006 Msa0607350:CDS 40.0%
AGATTGTTGTGTCTGAGAGT+GGG + chr4_2:54151988-54152007 Msa0607350:CDS 40.0%
GTTCGATGAAAATCAGAAGC+CGG + chr4_2:54152142-54152161 Msa0607350:CDS 40.0%
TTCGATGAAAATCAGAAGCC+GGG + chr4_2:54152143-54152162 Msa0607350:CDS 40.0%
! TCTGAAATTGAGCGACACTT+TGG + chr4_2:54152167-54152186 Msa0607350:CDS 40.0%
GATGCACGTACCTTACTTAT+TGG - chr4_2:54152321-54152340 None:intergenic 40.0%
ACCCGTAAATTCTAATGCAG+TGG - chr4_2:54150924-54150943 None:intergenic 40.0%
TCCTCTCTCTCTAATTAGCA+TGG + chr4_2:54150953-54150972 Msa0607350:intron 40.0%
TCCATGCTAATTAGAGAGAG+AGG - chr4_2:54150957-54150976 None:intergenic 40.0%
!! GGTGTTTGTTATGGAGGAAA+TGG + chr4_2:54151318-54151337 Msa0607350:CDS 40.0%
CTTGGTCTGGATTGTATATG+CGG - chr4_2:54151407-54151426 None:intergenic 40.0%
GTGATTTGAGAACGTCGTTT+GGG - chr4_2:54151479-54151498 None:intergenic 40.0%
AGTGATTTGAGAACGTCGTT+TGG - chr4_2:54151480-54151499 None:intergenic 40.0%
TCGCGGTAGACACTTTAATT+TGG - chr4_2:54151680-54151699 None:intergenic 40.0%
GCGATAGACACAACACTAAT+TGG + chr4_2:54151696-54151715 Msa0607350:CDS 40.0%
! CTAAAAACACCGTCTTTTGG+TGG - chr4_2:54151732-54151751 None:intergenic 40.0%
! TCGCTAAAAACACCGTCTTT+TGG - chr4_2:54151735-54151754 None:intergenic 40.0%
TATGCTGCTCTTGAGAAAGT+AGG + chr4_2:54151951-54151970 Msa0607350:CDS 40.0%
ATGCTGCTCTTGAGAAAGTA+GGG + chr4_2:54151952-54151971 Msa0607350:CDS 40.0%
AAGATTGTTGTGTCTGAGAG+TGG + chr4_2:54151987-54152006 Msa0607350:CDS 40.0%
AGATTGTTGTGTCTGAGAGT+GGG + chr4_2:54151988-54152007 Msa0607350:CDS 40.0%
GTTCGATGAAAATCAGAAGC+CGG + chr4_2:54152142-54152161 Msa0607350:CDS 40.0%
TTCGATGAAAATCAGAAGCC+GGG + chr4_2:54152143-54152162 Msa0607350:CDS 40.0%
! TCTGAAATTGAGCGACACTT+TGG + chr4_2:54152167-54152186 Msa0607350:CDS 40.0%
GATGCACGTACCTTACTTAT+TGG - chr4_2:54152321-54152340 None:intergenic 40.0%
CATAACAAACACCGACGGAT+TGG - chr4_2:54151311-54151330 None:intergenic 45.0%
! CAATCCGTCGGTGTTTGTTA+TGG + chr4_2:54151309-54151328 Msa0607350:CDS 45.0%
TCCTCCATAACAAACACCGA+CGG - chr4_2:54151316-54151335 None:intergenic 45.0%
TCTACCAACAAAGCAAGCAG+TGG + chr4_2:54151347-54151366 Msa0607350:CDS 45.0%
TCAACCACTGCTTGCTTTGT+TGG - chr4_2:54151354-54151373 None:intergenic 45.0%
CGCATATACAATCCAGACCA+AGG + chr4_2:54151405-54151424 Msa0607350:CDS 45.0%
TAAGGGCTTGGAGTATACCT+TGG - chr4_2:54151425-54151444 None:intergenic 45.0%
! TGTTTGAACCTCTAAGGGCT+TGG - chr4_2:54151437-54151456 None:intergenic 45.0%
TTTGTTGACCCAATCGTTCG+CGG - chr4_2:54151517-54151536 None:intergenic 45.0%
AGTTGGCAATGAAGTTCACC+CGG + chr4_2:54151578-54151597 Msa0607350:CDS 45.0%
! AATTTGTTTCGGCTGAACCC+GGG - chr4_2:54151599-54151618 None:intergenic 45.0%
! GAATTTGTTTCGGCTGAACC+CGG - chr4_2:54151600-54151619 None:intergenic 45.0%
ACGCTGCAAGTGGTTACATA+AGG + chr4_2:54151754-54151773 Msa0607350:CDS 45.0%
TGCTGCTCTTGAGAAAGTAG+GGG + chr4_2:54151953-54151972 Msa0607350:CDS 45.0%
TTAAGGGTGGAACTCCTAAG+AGG + chr4_2:54152084-54152103 Msa0607350:CDS 45.0%
ATAGGTCCATTAGGCCTCTT+AGG - chr4_2:54152101-54152120 None:intergenic 45.0%
TGTCGCTCAATTTCAGAACC+CGG - chr4_2:54152164-54152183 None:intergenic 45.0%
CATAACAAACACCGACGGAT+TGG - chr4_2:54151311-54151330 None:intergenic 45.0%
! CAATCCGTCGGTGTTTGTTA+TGG + chr4_2:54151309-54151328 Msa0607350:CDS 45.0%
TCCTCCATAACAAACACCGA+CGG - chr4_2:54151316-54151335 None:intergenic 45.0%
TCTACCAACAAAGCAAGCAG+TGG + chr4_2:54151347-54151366 Msa0607350:CDS 45.0%
TCAACCACTGCTTGCTTTGT+TGG - chr4_2:54151354-54151373 None:intergenic 45.0%
CGCATATACAATCCAGACCA+AGG + chr4_2:54151405-54151424 Msa0607350:CDS 45.0%
TAAGGGCTTGGAGTATACCT+TGG - chr4_2:54151425-54151444 None:intergenic 45.0%
! TGTTTGAACCTCTAAGGGCT+TGG - chr4_2:54151437-54151456 None:intergenic 45.0%
TTTGTTGACCCAATCGTTCG+CGG - chr4_2:54151517-54151536 None:intergenic 45.0%
AGTTGGCAATGAAGTTCACC+CGG + chr4_2:54151578-54151597 Msa0607350:CDS 45.0%
! AATTTGTTTCGGCTGAACCC+GGG - chr4_2:54151599-54151618 None:intergenic 45.0%
! GAATTTGTTTCGGCTGAACC+CGG - chr4_2:54151600-54151619 None:intergenic 45.0%
ACGCTGCAAGTGGTTACATA+AGG + chr4_2:54151754-54151773 Msa0607350:CDS 45.0%
TGCTGCTCTTGAGAAAGTAG+GGG + chr4_2:54151953-54151972 Msa0607350:CDS 45.0%
TTAAGGGTGGAACTCCTAAG+AGG + chr4_2:54152084-54152103 Msa0607350:CDS 45.0%
ATAGGTCCATTAGGCCTCTT+AGG - chr4_2:54152101-54152120 None:intergenic 45.0%
TGTCGCTCAATTTCAGAACC+CGG - chr4_2:54152164-54152183 None:intergenic 45.0%
CATGCAGTTGTCCAATCCGT+CGG + chr4_2:54151297-54151316 Msa0607350:intron 50.0%
!! TCCGTCGGTGTTTGTTATGG+AGG + chr4_2:54151312-54151331 Msa0607350:CDS 50.0%
GCTTGGAGTATACCTTGGTC+TGG - chr4_2:54151420-54151439 None:intergenic 50.0%
GGTATACTCCAAGCCCTTAG+AGG + chr4_2:54151426-54151445 Msa0607350:CDS 50.0%
GTTGGCAATGAAGTTCACCC+GGG + chr4_2:54151579-54151598 Msa0607350:CDS 50.0%
!!! GTTTTTAGCGACGCTGCAAG+TGG + chr4_2:54151744-54151763 Msa0607350:CDS 50.0%
GGGTAGACGTTAGCAAGAAG+TGG - chr4_2:54151813-54151832 None:intergenic 50.0%
GCTGCTCTTGAGAAAGTAGG+GGG + chr4_2:54151954-54151973 Msa0607350:CDS 50.0%
TTGTTGTGTCTGAGAGTGGG+TGG + chr4_2:54151991-54152010 Msa0607350:CDS 50.0%
GGAACTCCTAAGAGGCCTAA+TGG + chr4_2:54152092-54152111 Msa0607350:CDS 50.0%
GTACGTGCATCGTGCTCTTA+TGG + chr4_2:54152330-54152349 Msa0607350:three_prime_UTR 50.0%
CATGCAGTTGTCCAATCCGT+CGG + chr4_2:54151297-54151316 Msa0607350:intron 50.0%
!! TCCGTCGGTGTTTGTTATGG+AGG + chr4_2:54151312-54151331 Msa0607350:CDS 50.0%
GCTTGGAGTATACCTTGGTC+TGG - chr4_2:54151420-54151439 None:intergenic 50.0%
GGTATACTCCAAGCCCTTAG+AGG + chr4_2:54151426-54151445 Msa0607350:CDS 50.0%
GTTGGCAATGAAGTTCACCC+GGG + chr4_2:54151579-54151598 Msa0607350:CDS 50.0%
!!! GTTTTTAGCGACGCTGCAAG+TGG + chr4_2:54151744-54151763 Msa0607350:CDS 50.0%
GGGTAGACGTTAGCAAGAAG+TGG - chr4_2:54151813-54151832 None:intergenic 50.0%
GCTGCTCTTGAGAAAGTAGG+GGG + chr4_2:54151954-54151973 Msa0607350:CDS 50.0%
TTGTTGTGTCTGAGAGTGGG+TGG + chr4_2:54151991-54152010 Msa0607350:CDS 50.0%
GGAACTCCTAAGAGGCCTAA+TGG + chr4_2:54152092-54152111 Msa0607350:CDS 50.0%
GTACGTGCATCGTGCTCTTA+TGG + chr4_2:54152330-54152349 Msa0607350:three_prime_UTR 50.0%
! GAACGTCGTTTGGGACACCA+AGG - chr4_2:54151470-54151489 None:intergenic 55.0%
ATGCCCAAACCGCGAACGAT+TGG + chr4_2:54151505-54151524 Msa0607350:CDS 55.0%
TGCCCAAACCGCGAACGATT+GGG + chr4_2:54151506-54151525 Msa0607350:CDS 55.0%
GACCCAATCGTTCGCGGTTT+GGG - chr4_2:54151511-54151530 None:intergenic 55.0%
TGACCCAATCGTTCGCGGTT+TGG - chr4_2:54151512-54151531 None:intergenic 55.0%
TGGCCATCTCAAGGTGGAAC+TGG + chr4_2:54152011-54152030 Msa0607350:CDS 55.0%
GCTCCAGTTCCACCTTGAGA+TGG - chr4_2:54152017-54152036 None:intergenic 55.0%
GGTGGAACTGGAGCTAGTGT+TGG + chr4_2:54152023-54152042 Msa0607350:CDS 55.0%
! GAACGTCGTTTGGGACACCA+AGG - chr4_2:54151470-54151489 None:intergenic 55.0%
ATGCCCAAACCGCGAACGAT+TGG + chr4_2:54151505-54151524 Msa0607350:CDS 55.0%
TGCCCAAACCGCGAACGATT+GGG + chr4_2:54151506-54151525 Msa0607350:CDS 55.0%
GACCCAATCGTTCGCGGTTT+GGG - chr4_2:54151511-54151530 None:intergenic 55.0%
TGACCCAATCGTTCGCGGTT+TGG - chr4_2:54151512-54151531 None:intergenic 55.0%
TGGCCATCTCAAGGTGGAAC+TGG + chr4_2:54152011-54152030 Msa0607350:CDS 55.0%
GCTCCAGTTCCACCTTGAGA+TGG - chr4_2:54152017-54152036 None:intergenic 55.0%
GGTGGAACTGGAGCTAGTGT+TGG + chr4_2:54152023-54152042 Msa0607350:CDS 55.0%
GAGAGTGGGTGGCCATCTCA+AGG + chr4_2:54152002-54152021 Msa0607350:CDS 60.0%
AGTGGGTGGCCATCTCAAGG+TGG + chr4_2:54152005-54152024 Msa0607350:CDS 60.0%
GAGAGTGGGTGGCCATCTCA+AGG + chr4_2:54152002-54152021 Msa0607350:CDS 60.0%
AGTGGGTGGCCATCTCAAGG+TGG + chr4_2:54152005-54152024 Msa0607350:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 54150828 54152405 54150828 ID=Msa0607350;Name=Msa0607350
chr4_2 mRNA 54150828 54152405 54150828 ID=Msa0607350-mRNA-1;Parent=Msa0607350;Name=Msa0607350-mRNA-1;_AED=0.36;_eAED=0.36;_QI=53|1|1|1|0|0|2|167|331
chr4_2 exon 54150828 54150941 54150828 ID=Msa0607350-mRNA-1:exon:13529;Parent=Msa0607350-mRNA-1
chr4_2 exon 54151304 54152405 54151304 ID=Msa0607350-mRNA-1:exon:13530;Parent=Msa0607350-mRNA-1
chr4_2 five_prime_UTR 54150828 54150880 54150828 ID=Msa0607350-mRNA-1:five_prime_utr;Parent=Msa0607350-mRNA-1
chr4_2 CDS 54150881 54150941 54150881 ID=Msa0607350-mRNA-1:cds;Parent=Msa0607350-mRNA-1
chr4_2 CDS 54151304 54152238 54151304 ID=Msa0607350-mRNA-1:cds;Parent=Msa0607350-mRNA-1
chr4_2 three_prime_UTR 54152239 54152405 54152239 ID=Msa0607350-mRNA-1:three_prime_utr;Parent=Msa0607350-mRNA-1
Gene Sequence

>Msa0607350

ATGTCTATCATATTGCTGCTTATTGGAGTATTATTATCTTCCACTGCATTAGAATTTACGGTTGTCCAATCCGTCGGTGTTTGTTATGGAGGAAATGGAAATAATCTACCAACAAAGCAAGCAGTGGTTGATTTATACAAATCAAAAGGGATTGGTAAAATCCGCATATACAATCCAGACCAAGGTATACTCCAAGCCCTTAGAGGTTCAAACATAGAAGTAATCCTTGGTGTCCCAAACGACGTTCTCAAATCACTCACAAATGCCCAAACCGCGAACGATTGGGTCAACAAATACGTAAAAGCATACTCAAACGTCAAAATCAAATACATTGCAGTTGGCAATGAAGTTCACCCGGGTTCAGCCGAAACAAATTCAGTTCTTCCCGCAATGCAAAACATTCAAAAAGCAATATCTTCAGCAAACTTACAAGGCCAAATTAAAGTGTCTACCGCGATAGACACAACACTAATTGGAAAATCTTACCCACCAAAAGACGGTGTTTTTAGCGACGCTGCAAGTGGTTACATAAGGCCTATTGTTAACTTTTTAGTTAGCAACGGTTCACCACTTCTTGCTAACGTCTACCCTTATTTCTCATATGTTAATAACCAACAAAGCATTGGTCTTGATTATGCTTTGTTTACAAAACAGGGAAAGAATGAAGTTGGTTACCAAAACTTGTTTGATGCAATGTTGGATTCAATTTATGCTGCTCTTGAGAAAGTAGGGGGTTCAAATGTGAAGATTGTTGTGTCTGAGAGTGGGTGGCCATCTCAAGGTGGAACTGGAGCTAGTGTTGGAAATGCACAAACATATTATGGGAATTTGATTAAACATGTTAAGGGTGGAACTCCTAAGAGGCCTAATGGACCTATTGAGACTTATCTTTTTGCTATGTTCGATGAAAATCAGAAGCCGGGTTCTGAAATTGAGCGACACTTTGGTCTCTTTAGACCCGACAAATCACCTAAATATCAAATTAGTTTCAGTTGA
Protein sequence

>Msa0607350

MSIILLLIGVLLSSTALEFTVVQSVGVCYGGNGNNLPTKQAVVDLYKSKGIGKIRIYNPDQGILQALRGSNIEVILGVPNDVLKSLTNAQTANDWVNKYVKAYSNVKIKYIAVGNEVHPGSAETNSVLPAMQNIQKAISSANLQGQIKVSTAIDTTLIGKSYPPKDGVFSDAASGYIRPIVNFLVSNGSPLLANVYPYFSYVNNQQSIGLDYALFTKQGKNEVGYQNLFDAMLDSIYAALEKVGGSNVKIVVSESGWPSQGGTGASVGNAQTYYGNLIKHVKGGTPKRPNGPIETYLFAMFDENQKPGSEIERHFGLFRPDKSPKYQISFS*