Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0614200 | XP_013457178.1 | 87.879 | 132 | 6 | 1 | 1 | 132 | 1 | 122 | 1.14e-43 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0614200 | Msa0638680 | 0.906631 | 1.207262e-80 | -8.615850e-47 |
| Msa0614200 | Msa0697350 | 0.809084 | 2.253477e-50 | -8.615850e-47 |
| Msa0614200 | Msa1337450 | 0.816003 | 6.916032e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0614200 | MtrunA17_Chr4g0050141 | 87.879 | 132 | 6 | 1 | 1 | 132 | 1 | 122 | 1.05e-47 | 149 |
| Msa0614200 | MtrunA17_Chr4g0050091 | 78.030 | 132 | 18 | 3 | 1 | 132 | 1 | 121 | 1.95e-33 | 113 |
| Msa0614200 | MtrunA17_Chr4g0050101 | 67.290 | 107 | 25 | 3 | 1 | 104 | 1 | 100 | 4.34e-24 | 92.0 |
| Msa0614200 | MtrunA17_Chr4g0050061 | 73.485 | 132 | 22 | 2 | 1 | 132 | 1 | 119 | 1.42e-21 | 83.2 |
| Msa0614200 | MtrunA17_Chr4g0050131 | 81.034 | 116 | 14 | 2 | 1 | 116 | 1 | 108 | 4.10e-21 | 82.0 |
| Msa0614200 | MtrunA17_Chr6g0466601 | 72.581 | 62 | 17 | 0 | 44 | 105 | 23 | 84 | 1.36e-13 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAACAGATTCACATTTGC+TGG | 0.304728 | 4_2:-63131716 | Msa0614200:CDS |
| AGGAGATGGTGGAGAGTTGA+TGG | 0.345878 | 4_2:+63131847 | None:intergenic |
| TGGAGTGGCAACCGGTGATA+TGG | 0.388096 | 4_2:+63131964 | None:intergenic |
| CACAGTTGTGTTGATGTTTG+TGG | 0.412350 | 4_2:-63132038 | Msa0614200:CDS |
| GATGTTTGTGGCTACACTAT+TGG | 0.415229 | 4_2:-63132026 | Msa0614200:CDS |
| GCGGGAGAGTCGGATGAAGC+AGG | 0.419561 | 4_2:+63131821 | None:intergenic |
| AGATGGTGCCTGAGAGGACT+TGG | 0.427679 | 4_2:+63131940 | None:intergenic |
| ATCTCCTTCCGCTGTCTCAC+CGG | 0.436937 | 4_2:-63131921 | Msa0614200:CDS |
| GGAGTAGTAGAGATGGATGA+TGG | 0.440924 | 4_2:+63131779 | None:intergenic |
| GACTTGGGTGGAGTGGCAAC+CGG | 0.450462 | 4_2:+63131956 | None:intergenic |
| GGAGCGTTCTTCACAGGTAC+TGG | 0.455392 | 4_2:+63131887 | None:intergenic |
| CACTCCACCCAAGTCCTCTC+AGG | 0.464764 | 4_2:-63131948 | Msa0614200:CDS |
| TTGAGAAGGAGTAGTAGAGA+TGG | 0.490041 | 4_2:+63131772 | None:intergenic |
| CGGGAGAGTCGGATGAAGCA+GGG | 0.491491 | 4_2:+63131822 | None:intergenic |
| GGAGATGGAGCGTTCTTCAC+AGG | 0.508776 | 4_2:+63131881 | None:intergenic |
| TGCTGGATCTGCTGCCGTTG+TGG | 0.511630 | 4_2:-63131699 | Msa0614200:CDS |
| AGGAGAGACAGCGGGAGAGT+CGG | 0.511796 | 4_2:+63131811 | None:intergenic |
| GGGAGAGTCGGATGAAGCAG+GGG | 0.525944 | 4_2:+63131823 | None:intergenic |
| ATGGATGATGGAGTAACAGC+AGG | 0.530730 | 4_2:+63131791 | None:intergenic |
| AGTAACAGCAGGAGAGACAG+CGG | 0.550973 | 4_2:+63131802 | None:intergenic |
| ATGGCAGATGGTGAAGGAGA+TGG | 0.559733 | 4_2:+63131866 | None:intergenic |
| GAAGGTGAAGGTGCTTGAGA+AGG | 0.564001 | 4_2:+63131758 | None:intergenic |
| GGTGAGACAGCGGAAGGAGA+TGG | 0.565932 | 4_2:+63131923 | None:intergenic |
| ATCATCTCCAACCATATCAC+CGG | 0.577596 | 4_2:-63131975 | Msa0614200:CDS |
| TCCTTCCGCTGTCTCACCGG+TGG | 0.578332 | 4_2:-63131918 | Msa0614200:CDS |
| GTAACAGCAGGAGAGACAGC+GGG | 0.589547 | 4_2:+63131803 | None:intergenic |
| CTGAGAGGACTTGGGTGGAG+TGG | 0.594704 | 4_2:+63131949 | None:intergenic |
| GATGGTGCCTGAGAGGACTT+GGG | 0.602114 | 4_2:+63131941 | None:intergenic |
| GCCACCGGTGAGACAGCGGA+AGG | 0.606446 | 4_2:+63131917 | None:intergenic |
| GAATCTGTTCAAAGCGGCGG+CGG | 0.606447 | 4_2:+63131727 | None:intergenic |
| GAGTTGATGGCAGATGGTGA+AGG | 0.608419 | 4_2:+63131860 | None:intergenic |
| GGTGGAGAGTTGATGGCAGA+TGG | 0.609584 | 4_2:+63131854 | None:intergenic |
| GGAGAGTCGGATGAAGCAGG+GGG | 0.612386 | 4_2:+63131824 | None:intergenic |
| AGATGCCACCGGTGAGACAG+CGG | 0.622483 | 4_2:+63131913 | None:intergenic |
| TGAAGGAGATTGAGCAATGG+TGG | 0.623656 | 4_2:+63131997 | None:intergenic |
| GGAAGGAGATGGTGCCTGAG+AGG | 0.629137 | 4_2:+63131934 | None:intergenic |
| GTGGCAACCGGTGATATGGT+TGG | 0.632011 | 4_2:+63131968 | None:intergenic |
| TGTGAATCTGTTCAAAGCGG+CGG | 0.637759 | 4_2:+63131724 | None:intergenic |
| GCGGCGGCGGAGTTATCAGA+AGG | 0.647391 | 4_2:+63131740 | None:intergenic |
| GGTACTGGAGGAGATGCCAC+CGG | 0.659674 | 4_2:+63131902 | None:intergenic |
| GCGGAGTTATCAGAAGGTGA+AGG | 0.662844 | 4_2:+63131746 | None:intergenic |
| GCGTTCTTCACAGGTACTGG+AGG | 0.664174 | 4_2:+63131890 | None:intergenic |
| GATATGGTTGGAGATGATGA+AGG | 0.665449 | 4_2:+63131980 | None:intergenic |
| AGCAGCAGCGAAAACCACAA+CGG | 0.677280 | 4_2:+63131685 | None:intergenic |
| TGATGAAGGAGATTGAGCAA+TGG | 0.691682 | 4_2:+63131994 | None:intergenic |
| GGTGCCTGAGAGGACTTGGG+TGG | 0.711883 | 4_2:+63131944 | None:intergenic |
| AAATGTGAATCTGTTCAAAG+CGG | 0.718772 | 4_2:+63131721 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATGTGAATCTGTTCAAAG+CGG | + | chr4_2:63132004-63132023 | None:intergenic | 30.0% | |
| TTGAACAGATTCACATTTGC+TGG | - | chr4_2:63132006-63132025 | Msa0614200:CDS | 35.0% | |
| ! | CACAGTTGTGTTGATGTTTG+TGG | - | chr4_2:63131684-63131703 | Msa0614200:CDS | 40.0% |
| GATGTTTGTGGCTACACTAT+TGG | - | chr4_2:63131696-63131715 | Msa0614200:CDS | 40.0% | |
| TGATGAAGGAGATTGAGCAA+TGG | + | chr4_2:63131731-63131750 | None:intergenic | 40.0% | |
| GATATGGTTGGAGATGATGA+AGG | + | chr4_2:63131745-63131764 | None:intergenic | 40.0% | |
| ATCATCTCCAACCATATCAC+CGG | - | chr4_2:63131747-63131766 | Msa0614200:CDS | 40.0% | |
| TTGAGAAGGAGTAGTAGAGA+TGG | + | chr4_2:63131953-63131972 | None:intergenic | 40.0% | |
| TGAAGGAGATTGAGCAATGG+TGG | + | chr4_2:63131728-63131747 | None:intergenic | 45.0% | |
| !! | ATGGATGATGGAGTAACAGC+AGG | + | chr4_2:63131934-63131953 | None:intergenic | 45.0% |
| GGAGTAGTAGAGATGGATGA+TGG | + | chr4_2:63131946-63131965 | None:intergenic | 45.0% | |
| TGTGAATCTGTTCAAAGCGG+CGG | + | chr4_2:63132001-63132020 | None:intergenic | 45.0% | |
| ATGGCAGATGGTGAAGGAGA+TGG | + | chr4_2:63131859-63131878 | None:intergenic | 50.0% | |
| !! | GAGTTGATGGCAGATGGTGA+AGG | + | chr4_2:63131865-63131884 | None:intergenic | 50.0% |
| !! | AGGAGATGGTGGAGAGTTGA+TGG | + | chr4_2:63131878-63131897 | None:intergenic | 50.0% |
| AGTAACAGCAGGAGAGACAG+CGG | + | chr4_2:63131923-63131942 | None:intergenic | 50.0% | |
| !! | GAAGGTGAAGGTGCTTGAGA+AGG | + | chr4_2:63131967-63131986 | None:intergenic | 50.0% |
| GCGGAGTTATCAGAAGGTGA+AGG | + | chr4_2:63131979-63131998 | None:intergenic | 50.0% | |
| AGCAGCAGCGAAAACCACAA+CGG | + | chr4_2:63132040-63132059 | None:intergenic | 50.0% | |
| GTGGCAACCGGTGATATGGT+TGG | + | chr4_2:63131757-63131776 | None:intergenic | 55.0% | |
| TGGAGTGGCAACCGGTGATA+TGG | + | chr4_2:63131761-63131780 | None:intergenic | 55.0% | |
| GATGGTGCCTGAGAGGACTT+GGG | + | chr4_2:63131784-63131803 | None:intergenic | 55.0% | |
| AGATGGTGCCTGAGAGGACT+TGG | + | chr4_2:63131785-63131804 | None:intergenic | 55.0% | |
| ATCTCCTTCCGCTGTCTCAC+CGG | - | chr4_2:63131801-63131820 | Msa0614200:CDS | 55.0% | |
| !! | GCGTTCTTCACAGGTACTGG+AGG | + | chr4_2:63131835-63131854 | None:intergenic | 55.0% |
| ! | GGAGCGTTCTTCACAGGTAC+TGG | + | chr4_2:63131838-63131857 | None:intergenic | 55.0% |
| ! | GGAGATGGAGCGTTCTTCAC+AGG | + | chr4_2:63131844-63131863 | None:intergenic | 55.0% |
| !! | GGTGGAGAGTTGATGGCAGA+TGG | + | chr4_2:63131871-63131890 | None:intergenic | 55.0% |
| GTAACAGCAGGAGAGACAGC+GGG | + | chr4_2:63131922-63131941 | None:intergenic | 55.0% | |
| GAATCTGTTCAAAGCGGCGG+CGG | + | chr4_2:63131998-63132017 | None:intergenic | 55.0% | |
| GACTTGGGTGGAGTGGCAAC+CGG | + | chr4_2:63131769-63131788 | None:intergenic | 60.0% | |
| CTGAGAGGACTTGGGTGGAG+TGG | + | chr4_2:63131776-63131795 | None:intergenic | 60.0% | |
| CACTCCACCCAAGTCCTCTC+AGG | - | chr4_2:63131774-63131793 | Msa0614200:CDS | 60.0% | |
| GGAAGGAGATGGTGCCTGAG+AGG | + | chr4_2:63131791-63131810 | None:intergenic | 60.0% | |
| GGTGAGACAGCGGAAGGAGA+TGG | + | chr4_2:63131802-63131821 | None:intergenic | 60.0% | |
| AGATGCCACCGGTGAGACAG+CGG | + | chr4_2:63131812-63131831 | None:intergenic | 60.0% | |
| ! | GGTACTGGAGGAGATGCCAC+CGG | + | chr4_2:63131823-63131842 | None:intergenic | 60.0% |
| GATGAAGCAGGGGGAGGAGA+TGG | + | chr4_2:63131892-63131911 | None:intergenic | 60.0% | |
| GGAGAGTCGGATGAAGCAGG+GGG | + | chr4_2:63131901-63131920 | None:intergenic | 60.0% | |
| GGGAGAGTCGGATGAAGCAG+GGG | + | chr4_2:63131902-63131921 | None:intergenic | 60.0% | |
| CGGGAGAGTCGGATGAAGCA+GGG | + | chr4_2:63131903-63131922 | None:intergenic | 60.0% | |
| AGGAGAGACAGCGGGAGAGT+CGG | + | chr4_2:63131914-63131933 | None:intergenic | 60.0% | |
| TGCTGGATCTGCTGCCGTTG+TGG | - | chr4_2:63132023-63132042 | Msa0614200:CDS | 60.0% | |
| GGTGCCTGAGAGGACTTGGG+TGG | + | chr4_2:63131781-63131800 | None:intergenic | 65.0% | |
| TCCTTCCGCTGTCTCACCGG+TGG | - | chr4_2:63131804-63131823 | Msa0614200:CDS | 65.0% | |
| GAAGCAGGGGGAGGAGATGG+TGG | + | chr4_2:63131889-63131908 | None:intergenic | 65.0% | |
| GAGTCGGATGAAGCAGGGGG+AGG | + | chr4_2:63131898-63131917 | None:intergenic | 65.0% | |
| GCGGGAGAGTCGGATGAAGC+AGG | + | chr4_2:63131904-63131923 | None:intergenic | 65.0% | |
| GCGGCGGCGGAGTTATCAGA+AGG | + | chr4_2:63131985-63132004 | None:intergenic | 65.0% | |
| GCCACCGGTGAGACAGCGGA+AGG | + | chr4_2:63131808-63131827 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 63131673 | 63132071 | 63131673 | ID=Msa0614200;Name=Msa0614200 |
| chr4_2 | mRNA | 63131673 | 63132071 | 63131673 | ID=Msa0614200-mRNA-1;Parent=Msa0614200;Name=Msa0614200-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|132 |
| chr4_2 | exon | 63131673 | 63132071 | 63131673 | ID=Msa0614200-mRNA-1:exon:17577;Parent=Msa0614200-mRNA-1 |
| chr4_2 | CDS | 63131673 | 63132071 | 63131673 | ID=Msa0614200-mRNA-1:cds;Parent=Msa0614200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
