AlfalfaGEDB | Msa0614530

M. sativa cultivar zhongmu-4 / Msa0614530

KEGG: -
GO: -
PFAM: PF00226.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0614530	XP_013457209.1	91.803	183	9	2	1	181	1	179	4.64e-105	309

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0614530	sp\|Q9SDN0\|DNJ20_ARATH	38.462	130	68	4	55	175	66	192	1.26e-20	87.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0614530	Msa1225100	0.805792	1.124738e-49	-8.615850e-47
Msa0614530	Msa1329870	0.806634	7.479676e-50	-8.615850e-47
Msa0614530	Msa1405220	0.813287	2.762873e-51	-8.615850e-47
Msa0614530	Msa1457160	0.803178	3.946914e-49	-8.615850e-47
Msa0614530	Msa1461550	0.800332	1.514599e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0614530	MtrunA17_Chr4g0050581	91.803	183	9	2	1	181	1	179	4.26e-109	309
Msa0614530	MtrunA17_Chr2g0278961	55.901	161	49	6	5	161	6	148	2.66e-45	147
Msa0614530	MtrunA17_Chr2g0278951	54.118	170	55	7	5	169	6	157	3.06e-45	147
Msa0614530	MtrunA17_Chr8g0378161	33.684	190	98	9	9	175	5	189	2.68e-21	86.3
Msa0614530	MtrunA17_Chr3g0103131	42.553	94	48	4	39	128	42	133	1.33e-12	62.4
Msa0614530	MtrunA17_Chr2g0328581	41.026	78	41	2	45	122	65	137	3.37e-12	62.4
Msa0614530	MtrunA17_Chr2g0282741	41.250	80	42	3	53	130	2	78	4.90e-12	63.2
Msa0614530	MtrunA17_Chr5g0399201	45.455	66	35	1	56	121	13	77	1.39e-11	62.0
Msa0614530	MtrunA17_Chr2g0333221	41.667	84	42	3	54	135	69	147	1.94e-11	61.6
Msa0614530	MtrunA17_Chr7g0253281	33.333	150	88	4	1	146	3	144	2.41e-11	60.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0614530	AT4G13830.2	38.462	130	68	4	55	175	66	192	1.28e-21	87.4
Msa0614530	AT4G13830.1	47.222	72	36	2	55	126	66	135	4.64e-14	66.6
Msa0614530	AT4G13830.3	47.222	72	36	2	55	126	66	135	7.02e-14	66.6
Msa0614530	AT3G62600.1	46.753	77	35	3	53	127	24	96	1.01e-11	62.8
Msa0614530	AT2G20560.1	37.500	80	45	3	53	130	2	78	1.83e-11	62.0
Msa0614530	AT1G28210.1	40.426	94	49	4	55	147	48	135	2.84e-11	61.6
Msa0614530	AT1G28210.3	40.426	94	49	4	55	147	48	135	2.92e-11	61.6
Msa0614530	AT1G28210.2	40.426	94	49	4	55	147	48	135	4.13e-11	61.2
Msa0614530	AT5G59610.2	33.871	124	57	4	19	123	19	136	6.95e-11	59.7
Msa0614530	AT4G28480.2	36.905	84	48	3	53	134	2	82	7.42e-11	60.1

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Find 28 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTCTAGTAGACGTAT+GGG	0.582302	4_2:+63594992	Msa0614530:CDS
AAAGAGAGCTTATAGATCAA+TGG	0.492226	4_2:+63594745	Msa0614530:CDS
AAATTTCTACAAAATGTTGT+CGG	0.490774	4_2:+63594691	Msa0614530:CDS
AAGTAGGTGGCAAGAACAAG+TGG	0.619538	4_2:+63594958	Msa0614530:CDS
AAGTGGTTGAGTTGAAGAAA+AGG	0.458554	4_2:+63594975	Msa0614530:CDS
AATGCTTAGGCTTGAGGTTA+AGG	0.338721	4_2:-63594543	None:intergenic
AGATGATTACTAGAGAGTTG+TGG	0.607411	4_2:-63594593	None:intergenic
AGTAGACGTATGGGACAAAA+AGG	0.403585	4_2:+63595001	Msa0614530:CDS
ATATTGTAAATGATGAGATA+TGG	0.436536	4_2:+63594933	Msa0614530:CDS
ATGAGATATGGAGAAGTAGG+TGG	0.638622	4_2:+63594945	Msa0614530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTAGAAATTTGATCCATTCT+TGG	-	chr4_2:63594681-63594700	None:intergenic	30.0%
AAAGAGAGCTTATAGATCAA+TGG	+	chr4_2:63594745-63594764	Msa0614530:CDS	30.0%
AGATGATTACTAGAGAGTTG+TGG	-	chr4_2:63594596-63594615	None:intergenic	35.0%
TCATATTGTTCACGAAGCAT+AGG	-	chr4_2:63594872-63594891	None:intergenic	35.0%
ATGATGAGATATGGAGAAGT+AGG	+	chr4_2:63594942-63594961	Msa0614530:CDS	35.0%
AAAAGGTCTAGTAGACGTAT+GGG	+	chr4_2:63594992-63595011	Msa0614530:CDS	35.0%
AATGCTTAGGCTTGAGGTTA+AGG	-	chr4_2:63594546-63594565	None:intergenic	40.0%
GATTACTAGAGAGTTGTGGA+AGG	-	chr4_2:63594592-63594611	None:intergenic	40.0%
GTTGCAAAGTGTACATGACA+AGG	-	chr4_2:63594623-63594642	None:intergenic	40.0%
CTAGTGGAAGAAACCAAGAA+TGG	+	chr4_2:63594665-63594684	Msa0614530:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	63594530	63595075	63594530	ID=Msa0614530;Name=Msa0614530
chr4_2	mRNA	63594530	63595075	63594530	ID=Msa0614530-mRNA-1;Parent=Msa0614530;Name=Msa0614530-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|181
chr4_2	exon	63594530	63595075	63594530	ID=Msa0614530-mRNA-1:exon:17787;Parent=Msa0614530-mRNA-1
chr4_2	CDS	63594530	63595075	63594530	ID=Msa0614530-mRNA-1:cds;Parent=Msa0614530-mRNA-1

Gene Sequence

>Msa0614530

ATGAATGCTTCATCCTTAACCTCAAGCCTAAGCATTTCAAATTCAAAGCAATTTCATCACCTTCCACAACTCTCTAGTAATCATCTAAGACCTTGTCATGTACACTTTGCAACAATTTCGTGTAGAGCAACAAAGCTAGTGGAAGAAACCAAGAATGGATCAAATTTCTACAAAATGTTGTCGGTGAATCCGAAAAGCGCGACAATGGAAGAAATAAAGAGAGCTTATAGATCAATGGCACTTCAGTATCATCCTGATGTGTGTCATGATCCTTCCATGAAAGAGGAATCAACTAAAATTTTTGTTCGATTAAATGCAGCTTATGAAACTTTGTCAAATCCTATGCTTCGTGAACAATATGATAGTGAATTAGGTTTGAGAAACAACATGATGAATAATAATAATATTGTAAATGATGAGATATGGAGAAGTAGGTGGCAAGAACAAGTGGTTGAGTTGAAGAAAAGGTCTAGTAGACGTATGGGACAAAAAGGAAGGTCTTGGGGTAGCAGAATGAGGACACAAAGCATGAAAGATCGGAACTAA

Protein sequence

>Msa0614530

MNASSLTSSLSISNSKQFHHLPQLSSNHLRPCHVHFATISCRATKLVEETKNGSNFYKMLSVNPKSATMEEIKRAYRSMALQYHPDVCHDPSMKEESTKIFVRLNAAYETLSNPMLREQYDSELGLRNNMMNNNNIVNDEIWRSRWQEQVVELKKRSSRRMGQKGRSWGSRMRTQSMKDRN*