AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0616310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0616310 MtrunA17_Chr8g0375751 97.826 414 9 0 1 414 7 420 0.0 845
Msa0616310 MtrunA17_Chr1g0165011 69.213 445 105 5 1 414 9 452 0.0 615
Msa0616310 MtrunA17_Chr6g0479511 55.425 341 131 2 55 375 47 386 1.31e-141 409
Msa0616310 MtrunA17_Chr7g0263111 42.643 401 192 8 2 376 11 399 2.59e-101 308
Msa0616310 MtrunA17_Chr6g0452181 45.787 356 165 6 52 380 54 408 8.93e-101 307
Msa0616310 MtrunA17_Chr8g0382571 55.649 239 101 2 140 375 213 449 5.41e-86 270
Msa0616310 MtrunA17_Chr8g0382571 51.852 81 37 2 45 124 29 108 7.39e-18 85.5
Msa0616310 MtrunA17_Chr1g0185281 51.938 258 114 4 118 371 269 520 6.54e-84 267
Msa0616310 MtrunA17_Chr1g0185281 48.864 88 40 2 37 124 79 161 1.45e-17 84.7
Msa0616310 MtrunA17_Chr1g0205121 53.498 243 108 2 132 371 307 547 6.75e-84 267
Msa0616310 MtrunA17_Chr1g0205121 53.086 81 35 2 46 124 82 161 7.37e-19 89.0
Msa0616310 MtrunA17_Chr5g0394901 54.043 235 103 2 140 371 354 586 9.41e-84 268
Msa0616310 MtrunA17_Chr5g0394901 51.852 81 36 2 46 124 84 163 1.99e-18 87.4
Msa0616310 MtrunA17_Chr8g0355021 51.464 239 108 3 140 373 227 462 7.21e-81 257
Msa0616310 MtrunA17_Chr8g0355021 47.945 73 37 1 52 124 60 131 1.88e-15 78.2
Msa0616310 MtrunA17_Chr7g0260081 54.310 232 101 2 143 371 291 520 8.72e-80 256
Msa0616310 MtrunA17_Chr7g0260081 54.930 71 31 1 54 124 92 161 1.79e-16 81.3
Msa0616310 MtrunA17_Chr4g0054941 45.645 287 141 3 93 373 264 541 2.07e-77 250
Msa0616310 MtrunA17_Chr4g0054941 38.824 85 52 0 41 125 60 144 7.90e-14 73.2
Msa0616310 MtrunA17_Chr6g0466801 51.230 244 114 2 143 386 372 610 3.67e-77 251
Msa0616310 MtrunA17_Chr6g0466801 52.113 71 33 1 55 125 99 168 1.03e-15 79.3
Msa0616310 MtrunA17_Chr1g0201271 48.485 264 128 4 136 396 367 625 4.33e-76 248
Msa0616310 MtrunA17_Chr1g0201271 42.529 87 49 1 39 125 87 172 9.67e-14 73.2
Msa0616310 MtrunA17_Chr5g0400311 45.614 285 152 2 89 373 317 598 1.75e-74 244
Msa0616310 MtrunA17_Chr5g0400311 44.330 97 49 2 41 133 93 188 2.32e-17 84.3
Msa0616310 MtrunA17_Chr4g0054951 46.212 264 140 1 110 373 280 541 6.16e-74 241
Msa0616310 MtrunA17_Chr4g0054951 41.176 85 49 1 41 125 63 146 6.79e-13 70.5
Msa0616310 MtrunA17_Chr1g0189231 27.098 417 225 13 55 414 528 922 9.28e-35 137
Msa0616310 MtrunA17_Chr6g0488541 23.790 248 142 6 19 232 499 733 2.96e-13 72.0
Msa0616310 MtrunA17_Chr6g0488541 31.461 178 91 7 244 399 805 973 4.26e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0616310 AT2G01900.3 64.268 403 131 6 1 391 9 410 0.0 535
Msa0616310 AT2G01900.1 64.268 403 131 6 1 391 1 402 0.0 534
Msa0616310 AT2G01900.2 62.779 403 128 7 1 391 1 393 0.0 515
Msa0616310 AT2G37440.1 46.318 421 176 10 2 376 11 427 7.84e-109 329
Msa0616310 AT2G37440.3 46.318 421 176 10 2 376 11 427 7.84e-109 329
Msa0616310 AT2G37440.2 48.857 350 146 5 56 376 1 346 1.04e-101 308
Msa0616310 AT5G65090.2 56.485 239 99 2 140 375 199 435 9.34e-88 274
Msa0616310 AT5G65090.2 45.783 83 44 1 55 137 11 92 6.97e-18 85.9
Msa0616310 AT5G65090.3 56.485 239 99 2 140 375 262 498 2.82e-87 275
Msa0616310 AT5G65090.3 45.783 83 44 1 55 137 74 155 1.15e-17 85.5
Msa0616310 AT5G65090.1 56.485 239 99 2 140 375 262 498 2.82e-87 275
Msa0616310 AT5G65090.1 45.783 83 44 1 55 137 74 155 1.15e-17 85.5
Msa0616310 AT2G32010.2 53.386 251 112 2 143 390 329 577 1.78e-85 272
Msa0616310 AT2G32010.2 57.333 75 31 1 50 124 87 160 1.55e-19 91.3
Msa0616310 AT2G32010.1 53.386 251 112 2 143 390 329 577 1.78e-85 272
Msa0616310 AT2G32010.1 57.333 75 31 1 50 124 87 160 1.55e-19 91.3
Msa0616310 AT2G32010.4 53.386 251 112 2 143 390 329 577 1.78e-85 272
Msa0616310 AT2G32010.4 57.333 75 31 1 50 124 87 160 1.55e-19 91.3
Msa0616310 AT2G32010.3 53.386 251 112 2 143 390 329 577 1.78e-85 272
Msa0616310 AT2G32010.3 57.333 75 31 1 50 124 87 160 1.55e-19 91.3
Msa0616310 AT1G05470.2 56.034 232 97 2 143 371 352 581 7.64e-85 271
Msa0616310 AT1G05470.2 57.333 75 31 1 50 124 88 161 2.36e-19 90.9
Msa0616310 AT1G05470.1 56.034 232 97 2 143 371 352 581 7.64e-85 271
Msa0616310 AT1G05470.1 57.333 75 31 1 50 124 88 161 2.36e-19 90.9
Msa0616310 AT5G04980.4 52.743 237 108 2 140 373 157 392 2.03e-82 258
Msa0616310 AT5G04980.2 52.743 237 108 2 140 373 221 456 4.67e-82 259
Msa0616310 AT5G04980.2 50.746 67 32 1 55 121 39 104 2.23e-13 72.0
Msa0616310 AT5G04980.1 52.743 237 108 2 140 373 192 427 4.85e-82 259
Msa0616310 AT5G04980.1 50.746 67 32 1 55 121 10 75 2.20e-13 72.0
Msa0616310 AT5G04980.3 52.743 237 108 2 140 373 192 427 4.85e-82 259
Msa0616310 AT5G04980.3 50.746 67 32 1 55 121 10 75 2.20e-13 72.0
Msa0616310 AT3G63240.2 53.600 250 106 4 126 371 267 510 5.29e-81 259
Msa0616310 AT3G63240.2 55.714 70 30 1 55 124 89 157 1.81e-16 81.6
Msa0616310 AT3G63240.1 53.600 250 106 4 126 371 267 510 5.29e-81 259
Msa0616310 AT3G63240.1 55.714 70 30 1 55 124 89 157 1.81e-16 81.6
Msa0616310 AT1G34120.5 49.593 246 118 2 143 386 185 426 1.11e-75 242
Msa0616310 AT1G34120.1 49.593 246 118 2 143 386 336 577 1.00e-73 241
Msa0616310 AT1G34120.1 40.000 100 59 1 39 138 86 184 2.12e-14 75.5
Msa0616310 AT1G34120.2 49.593 246 118 2 143 386 340 581 1.44e-73 241
Msa0616310 AT1G34120.2 40.000 100 59 1 39 138 90 188 2.15e-14 75.5
Msa0616310 AT4G18010.1 51.515 231 110 1 143 373 392 620 2.01e-73 242
Msa0616310 AT4G18010.1 43.158 95 53 1 41 135 98 191 2.54e-17 84.7
Msa0616310 AT1G34120.3 49.593 246 117 3 143 386 340 580 7.98e-72 236
Msa0616310 AT1G34120.3 40.000 100 59 1 39 138 90 188 2.19e-14 75.5
Msa0616310 AT1G71710.2 45.714 245 129 2 143 387 402 642 1.81e-69 231
Msa0616310 AT1G71710.2 47.126 87 45 1 39 125 104 189 3.16e-18 87.4
Msa0616310 AT1G71710.1 45.714 245 129 2 143 387 411 651 2.20e-69 231
Msa0616310 AT1G71710.1 47.126 87 45 1 39 125 113 198 3.25e-18 87.4
Msa0616310 AT4G18010.2 50.000 192 94 1 143 334 392 581 2.53e-55 192
Msa0616310 AT4G18010.2 43.158 95 53 1 41 135 98 191 2.18e-17 84.7
Msa0616310 AT1G34120.4 49.640 139 68 1 143 279 336 474 9.35e-38 142
Msa0616310 AT1G34120.4 40.000 100 59 1 39 138 86 184 2.04e-14 75.1
Msa0616310 AT1G65580.2 27.818 417 221 14 55 411 273 669 7.11e-36 140
Msa0616310 AT1G65580.1 27.644 416 223 13 55 411 539 935 1.08e-35 140
Msa0616310 AT1G05630.2 26.952 397 214 10 55 397 572 946 4.07e-33 132
Msa0616310 AT1G05630.3 27.812 320 179 7 55 340 572 873 1.24e-32 130
Msa0616310 AT2G31830.1 25.598 418 231 11 55 412 581 978 1.35e-31 128
Msa0616310 AT2G31830.2 25.598 418 231 11 55 412 581 978 1.61e-31 127
Msa0616310 AT2G43900.2 26.791 321 182 8 55 340 577 879 7.03e-30 123
Msa0616310 AT2G43900.1 24.586 423 234 12 55 414 577 977 7.52e-30 122
Msa0616310 AT2G31830.3 27.187 320 181 7 55 340 581 882 2.17e-29 121
Msa0616310 AT1G47510.4 31.527 203 111 7 171 365 81 263 6.59e-19 86.7
Msa0616310 AT1G47510.1 26.480 321 174 12 56 365 56 325 1.30e-18 86.7
Msa0616310 AT1G05630.1 28.495 186 112 4 55 233 572 743 2.51e-17 85.1
Msa0616310 AT1G05630.1 28.947 190 106 5 229 397 799 980 6.95e-12 68.2
Msa0616310 AT1G47510.2 34.711 121 65 3 252 365 149 262 2.24e-11 64.3

Find 77 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATGGAATTCCATTTAT+TGG 0.159218 4_2:+66125035 None:intergenic
CCAAATTCGGATATATATTA+TGG 0.203896 4_2:-66123298 Msa0616310:CDS
ACAATGAAATTTCCAATAAA+TGG 0.214880 4_2:-66125047 Msa0616310:CDS
CCATAATATATATCCGAATT+TGG 0.241692 4_2:+66123298 None:intergenic
CTTGTATTTGTGTTTGTCCT+TGG 0.283816 4_2:+66125445 None:intergenic
GTGTGTAGCCATCTAGCTTC+AGG 0.308501 4_2:-66124264 Msa0616310:CDS
ACGCTTACTTGTAGAGAAAA+AGG 0.326969 4_2:-66123524 Msa0616310:CDS
TGCTTGGAGATTTAAATTAT+AGG 0.331581 4_2:-66123570 Msa0616310:CDS
TCTAAAAGCTTCAAATGTAT+TGG 0.332943 4_2:-66125079 Msa0616310:CDS
TCATGGTCTAGAATATTTCT+TGG 0.341551 4_2:+66124150 None:intergenic
AATCAGAAAAGCTTTGAATA+AGG 0.342999 4_2:-66125016 Msa0616310:CDS
TGTTTGTCCTTGGGATGATT+AGG 0.356217 4_2:+66125455 None:intergenic
CTAAAAGCTTCAAATGTATT+GGG 0.357957 4_2:-66125078 Msa0616310:CDS
TGTGGCATAATGAGATGTTT+AGG 0.368843 4_2:-66124847 Msa0616310:CDS
TTGTATTTGTGTTTGTCCTT+GGG 0.382205 4_2:+66125446 None:intergenic
CTACAAATATTGTCCAAATT+CGG 0.392628 4_2:-66123311 Msa0616310:CDS
TCCTGTGACATCTATGTACT+TGG 0.396131 4_2:-66125230 Msa0616310:CDS
TTAGGTGTGATAGAATAGTA+TGG 0.408260 4_2:-66122875 Msa0616310:intron
CGAATTTGGACAATATTTGT+AGG 0.419036 4_2:+66123312 None:intergenic
TTTAAACGTAGATGATTTGT+TGG 0.420397 4_2:-66125267 Msa0616310:CDS
AATCAAGACTATCAGATCAT+AGG 0.423602 4_2:-66122812 Msa0616310:CDS
GTAGAAAACAATCAAGCTTC+CGG 0.425807 4_2:-66122656 Msa0616310:CDS
TGTGTAGCCATCTAGCTTCA+GGG 0.426156 4_2:-66124263 Msa0616310:CDS
TAGGTAGGAGCAAATTTGAT+TGG 0.432972 4_2:+66123331 None:intergenic
TTCCTTATTAGAAAATGATC+AGG 0.438935 4_2:-66123494 Msa0616310:intron
TCTACGTTTAAATCTTCATC+TGG 0.441644 4_2:+66125278 None:intergenic
AGTAAGCGTGTCGTTTCCTC+TGG 0.445903 4_2:+66123538 None:intergenic
ATGAATGGCAACAATTTAAG+AGG 0.447227 4_2:-66123367 Msa0616310:CDS
ATCAGATCATAGGCCTGTTA+AGG 0.461445 4_2:-66122802 Msa0616310:CDS
TTGTTAATTTCTTACCAAGC+TGG 0.469234 4_2:+66123235 None:intergenic
TAACATGTGGCCTACATTAG+TGG 0.470394 4_2:-66125591 None:intergenic
TGCAGTCATGTAATATTGCT+TGG 0.474593 4_2:-66123586 Msa0616310:intron
ACTGATGGTGAATTTACAAC+CGG 0.475273 4_2:+66122637 None:intergenic
AGGATAATCTGCTATGAAGT+TGG 0.477080 4_2:+66125535 None:intergenic
ATGTATAATTAGCAAACAAA+TGG 0.477259 4_2:-66124947 Msa0616310:CDS
CAACCTATGCATGACACACT+TGG 0.485601 4_2:+66124868 None:intergenic
TTTGTTAGTACATGGAACAT+AGG 0.487262 4_2:-66125311 Msa0616310:CDS
TTGGAATATTCATATCTGTA+TGG 0.499275 4_2:-66124924 Msa0616310:CDS
TTTGGACAATATTTGTAGGT+AGG 0.503958 4_2:+66123316 None:intergenic
TGTGATAGAATAGTATGGTA+TGG 0.509257 4_2:-66122870 Msa0616310:CDS
TTTGTTTGCTAATTATACAT+TGG 0.521389 4_2:+66124950 None:intergenic
ACACTTGGATGTTGAATGAA+TGG 0.524578 4_2:+66124883 None:intergenic
AAACGTAGATGATTTGTTGG+AGG 0.529826 4_2:-66125264 Msa0616310:CDS
TTTCTTGGCAATTCAAACAA+AGG 0.536236 4_2:+66124165 None:intergenic
CTAATGATGGAGTTAATGAA+TGG 0.537149 4_2:-66123382 Msa0616310:CDS
AAGAAGGGGATGAGAAGTGT+AGG 0.538109 4_2:-66124236 Msa0616310:CDS
ATAATTAGCAAACAAATGGT+TGG 0.541121 4_2:-66124943 Msa0616310:CDS
AGAATAGTATGGTATGGAAA+GGG 0.542462 4_2:-66122864 Msa0616310:CDS
TTAGTACATGGAACATAGGT+GGG 0.544468 4_2:-66125307 Msa0616310:CDS
TATTATGGTTGCTGTTGTCA+TGG 0.545796 4_2:-66123283 Msa0616310:CDS
AATTCAAACAAAGGACCTCT+AGG 0.548525 4_2:+66124174 None:intergenic
TAGAATAGTATGGTATGGAA+AGG 0.552813 4_2:-66122865 Msa0616310:CDS
AATGGGAATGAAGCTAATGA+TGG 0.553046 4_2:-66123395 Msa0616310:intron
GTTAGTACATGGAACATAGG+TGG 0.556986 4_2:-66125308 Msa0616310:CDS
ATCTTATTAGCCACTAATGT+AGG 0.560756 4_2:+66125581 None:intergenic
AAGATACTCAAGAAGCGACT+AGG 0.561529 4_2:-66125563 Msa0616310:CDS
AGAAATAAGGGTTTCATCTG+AGG 0.563849 4_2:-66122766 Msa0616310:CDS
AAAAGAGGCGAGCACCAGCT+TGG 0.572374 4_2:-66123249 Msa0616310:intron
TCTGTGGTAGAAACTTCAAA+AGG 0.575675 4_2:+66122690 None:intergenic
CATCCAAGTGTGTCATGCAT+AGG 0.576317 4_2:-66124871 Msa0616310:CDS
ATGGCAACAATTTAAGAGGA+TGG 0.577966 4_2:-66123363 Msa0616310:CDS
AATTTAAGAGGATGGCATGA+AGG 0.581584 4_2:-66123355 Msa0616310:CDS
GTGTAGCCATCTAGCTTCAG+GGG 0.595621 4_2:-66124262 Msa0616310:CDS
CCCAAGTACATAGATGTCAC+AGG 0.603987 4_2:+66125229 None:intergenic
ACATTGGAAATCATCATGTG+TGG 0.610903 4_2:+66124966 None:intergenic
AACAAAGGTTCATCATAACT+AGG 0.611095 4_2:+66125515 None:intergenic
TGAAACAACTTGAGTACACA+AGG 0.618581 4_2:-66122839 Msa0616310:CDS
CCTGTGACATCTATGTACTT+GGG 0.620340 4_2:-66125229 Msa0616310:intron
AATGAGATGTTTAGGAAACA+AGG 0.621139 4_2:-66124839 Msa0616310:intron
AACAGTTCCTAATCATCCCA+AGG 0.629549 4_2:-66125462 Msa0616310:CDS
TCTACATACAAGTTCATCTG+TGG 0.630406 4_2:+66122674 None:intergenic
ATGTCTGCAAAATTCAACAA+AGG 0.633351 4_2:+66125500 None:intergenic
TCTAGGACTAGCTTTCCTAG+AGG 0.647556 4_2:-66124189 Msa0616310:CDS
TTATAGGATATCTCTTCCAG+AGG 0.665637 4_2:-66123554 Msa0616310:CDS
TATTCATATCTGTATGGACA+AGG 0.666150 4_2:-66124918 Msa0616310:CDS
AGTGTGTCATGCATAGGTTG+TGG 0.684284 4_2:-66124865 Msa0616310:CDS
TGTAGCCATCTAGCTTCAGG+GGG 0.696209 4_2:-66124261 Msa0616310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTAACATATTAATATTT+TGG + chr4_2:66124332-66124351 None:intergenic 10.0%
!!! TTTGTTTATTTTTCTTGTAA+AGG - chr4_2:66122900-66122919 Msa0616310:intron 15.0%
!!! TTTTCATGATTTATTATGAA+AGG + chr4_2:66124202-66124221 None:intergenic 15.0%
!!! TTAACATATTAATATTTTGG+AGG + chr4_2:66124329-66124348 None:intergenic 15.0%
!!! TTTAAAGAGAACTTTTTTAA+TGG + chr4_2:66124794-66124813 None:intergenic 15.0%
!!! TGTTTGTTAATCTTAATTTT+AGG - chr4_2:66125344-66125363 Msa0616310:intron 15.0%
!! ACAATATTAGAAATGTTAAG+AGG + chr4_2:66122880-66122899 None:intergenic 20.0%
!!! ATTCTAAACATGTTTTTCTA+TGG + chr4_2:66123067-66123086 None:intergenic 20.0%
!! ACAATGAAATTTCCAATAAA+TGG - chr4_2:66123190-66123209 Msa0616310:intron 20.0%
!!! TTTGTTTGCTAATTATACAT+TGG + chr4_2:66123290-66123309 None:intergenic 20.0%
!! ATGTATAATTAGCAAACAAA+TGG - chr4_2:66123290-66123309 Msa0616310:CDS 20.0%
!! ATATTAATTTCATATGGAGT+TGG + chr4_2:66123458-66123477 None:intergenic 20.0%
!! AAAATATCAAGCTTGAAATT+TGG - chr4_2:66123652-66123671 Msa0616310:intron 20.0%
!!! TTTTAGTCTTTTTAGACTAT+TGG + chr4_2:66123849-66123868 None:intergenic 20.0%
!! AGTAGTATTAGAAATCAAAT+TGG + chr4_2:66124135-66124154 None:intergenic 20.0%
!! TTATTAAATTAAACGCAAGA+GGG + chr4_2:66124172-66124191 None:intergenic 20.0%
!! ATTATTAAATTAAACGCAAG+AGG + chr4_2:66124173-66124192 None:intergenic 20.0%
!! CATAATAAATCATGAAAACA+AGG - chr4_2:66124204-66124223 Msa0616310:CDS 20.0%
!!! ATATTTACAACGTTTTTCTT+TGG + chr4_2:66124462-66124481 None:intergenic 20.0%
!! ACATTATGTGATTAATTATG+TGG + chr4_2:66124627-66124646 None:intergenic 20.0%
!!! AATTTTTTCTGCAGAAATAA+GGG - chr4_2:66125459-66125478 Msa0616310:CDS 20.0%
!! AAAGGATTTTTGTTAGTACA+TGG - chr4_2:66122918-66122937 Msa0616310:intron 25.0%
!! TTTAAACGTAGATGATTTGT+TGG - chr4_2:66122970-66122989 Msa0616310:intron 25.0%
!!! GTTCTGTTTTATTACAATTC+AGG - chr4_2:66123118-66123137 Msa0616310:intron 25.0%
!!! AATACATTTGAAGCTTTTAG+AGG + chr4_2:66123159-66123178 None:intergenic 25.0%
! TCTAAAAGCTTCAAATGTAT+TGG - chr4_2:66123158-66123177 Msa0616310:intron 25.0%
! CTAAAAGCTTCAAATGTATT+GGG - chr4_2:66123159-66123178 Msa0616310:intron 25.0%
! GATTATGGAATTCCATTTAT+TGG + chr4_2:66123205-66123224 None:intergenic 25.0%
!! ATTCAAAGCTTTTCTGATTA+TGG + chr4_2:66123220-66123239 None:intergenic 25.0%
!!! AATCAGAAAAGCTTTGAATA+AGG - chr4_2:66123221-66123240 Msa0616310:intron 25.0%
! ATAATTAGCAAACAAATGGT+TGG - chr4_2:66123294-66123313 Msa0616310:CDS 25.0%
! TTGGAATATTCATATCTGTA+TGG - chr4_2:66123313-66123332 Msa0616310:CDS 25.0%
!! AAGATTTTCAGTTAACCAAT+GGG + chr4_2:66123792-66123811 None:intergenic 25.0%
!! TAAGATTTTCAGTTAACCAA+TGG + chr4_2:66123793-66123812 None:intergenic 25.0%
!!! ATGTTGCTGAAATTTTTTCT+AGG - chr4_2:66124031-66124050 Msa0616310:intron 25.0%
!! TTCAAATTGTTACTTACTCA+TGG + chr4_2:66124107-66124126 None:intergenic 25.0%
! GTATTAGAAATCAAATTGGA+CGG + chr4_2:66124131-66124150 None:intergenic 25.0%
! ATTTCAGCCAACAAAATATT+TGG + chr4_2:66124231-66124250 None:intergenic 25.0%
! AGAATATTGATCATTAGTGA+CGG + chr4_2:66124279-66124298 None:intergenic 25.0%
! TCTGGTAGTATATAATATAG+TGG - chr4_2:66124413-66124432 Msa0616310:intron 25.0%
! CTGGTAGTATATAATATAGT+GGG - chr4_2:66124414-66124433 Msa0616310:intron 25.0%
! CTCATTAATCAATTAGTATG+TGG + chr4_2:66124540-66124559 None:intergenic 25.0%
! TGCTTGGAGATTTAAATTAT+AGG - chr4_2:66124667-66124686 Msa0616310:intron 25.0%
! TTCCTTATTAGAAAATGATC+AGG - chr4_2:66124743-66124762 Msa0616310:intron 25.0%
!! AACCTGATCATTTTCTAATA+AGG + chr4_2:66124748-66124767 None:intergenic 25.0%
! TCTAATTTAATTGCATCCAA+TGG - chr4_2:66124824-66124843 Msa0616310:intron 25.0%
! CTAATTTAATTGCATCCAAT+GGG - chr4_2:66124825-66124844 Msa0616310:intron 25.0%
! CTACAAATATTGTCCAAATT+CGG - chr4_2:66124926-66124945 Msa0616310:CDS 25.0%
! CCATAATATATATCCGAATT+TGG + chr4_2:66124942-66124961 None:intergenic 25.0%
! CCAAATTCGGATATATATTA+TGG - chr4_2:66124939-66124958 Msa0616310:CDS 25.0%
! ATGGAAAAAAGATAGAAAAG+AGG - chr4_2:66124973-66124992 Msa0616310:CDS 25.0%
! AATGTCATATATGTTTGTGT+CGG + chr4_2:66125071-66125090 None:intergenic 25.0%
! TCTTAAACTATTATCGATGT+CGG + chr4_2:66125106-66125125 None:intergenic 25.0%
! TCACTTTATAAGAATTTCGT+CGG - chr4_2:66125214-66125233 Msa0616310:intron 25.0%
!! TAAAAGTGTGTAGTATAAAG+TGG + chr4_2:66125253-66125272 None:intergenic 25.0%
!! TATACTACACACTTTTAACA+TGG - chr4_2:66125256-66125275 Msa0616310:CDS 25.0%
!!! CAATTTTTTCTGCAGAAATA+AGG - chr4_2:66125458-66125477 Msa0616310:CDS 25.0%
ATCTTATTAGCCACTAATGT+AGG + chr4_2:66122659-66122678 None:intergenic 30.0%
AACAAAGGTTCATCATAACT+AGG + chr4_2:66122725-66122744 None:intergenic 30.0%
ATGTCTGCAAAATTCAACAA+AGG + chr4_2:66122740-66122759 None:intergenic 30.0%
TTGTATTTGTGTTTGTCCTT+GGG + chr4_2:66122794-66122813 None:intergenic 30.0%
TTTGTTAGTACATGGAACAT+AGG - chr4_2:66122926-66122945 Msa0616310:intron 30.0%
TCTACGTTTAAATCTTCATC+TGG + chr4_2:66122962-66122981 None:intergenic 30.0%
TATGGTAGCTATACAAAACA+TGG + chr4_2:66123049-66123068 None:intergenic 30.0%
TATTCATATCTGTATGGACA+AGG - chr4_2:66123319-66123338 Msa0616310:CDS 30.0%
AATGAGATGTTTAGGAAACA+AGG - chr4_2:66123398-66123417 Msa0616310:intron 30.0%
!! TTATAGGCTTATAGCCTTAT+AGG + chr4_2:66123429-66123448 None:intergenic 30.0%
! ATGGAGTTGGTTATAGTTAT+AGG + chr4_2:66123445-66123464 None:intergenic 30.0%
ATATGACATATGTGAGGTTA+TGG + chr4_2:66123498-66123517 None:intergenic 30.0%
AGTGATATATGACATATGTG+AGG + chr4_2:66123504-66123523 None:intergenic 30.0%
!! TTGTACACCAATTAATTTGC+AGG - chr4_2:66123814-66123833 Msa0616310:intron 30.0%
ACTAAAATCATGCATCTCAT+TGG - chr4_2:66123862-66123881 Msa0616310:intron 30.0%
!! TTCTACACCAATTAATTTGC+AGG - chr4_2:66123903-66123922 Msa0616310:intron 30.0%
!! TCTACACCAATTAATTTGCA+GGG - chr4_2:66123904-66123923 Msa0616310:intron 30.0%
TTTCTTGGCAATTCAAACAA+AGG + chr4_2:66124075-66124094 None:intergenic 30.0%
! TCATGGTCTAGAATATTTCT+TGG + chr4_2:66124090-66124109 None:intergenic 30.0%
! ACAAGGTCCAAATATTTTGT+TGG - chr4_2:66124221-66124240 Msa0616310:CDS 30.0%
TGATCAATATTCTCCAACTT+TGG - chr4_2:66124286-66124305 Msa0616310:CDS 30.0%
!!! TTTGGAGGCATTTTTTCTAT+GGG + chr4_2:66124314-66124333 None:intergenic 30.0%
!!! TTTTGGAGGCATTTTTTCTA+TGG + chr4_2:66124315-66124334 None:intergenic 30.0%
TATACTACCAGAAAACAGAT+AGG + chr4_2:66124405-66124424 None:intergenic 30.0%
!! TAGTATATAATATAGTGGGC+CGG - chr4_2:66124418-66124437 Msa0616310:intron 30.0%
! CTAATGATGGAGTTAATGAA+TGG - chr4_2:66124855-66124874 Msa0616310:CDS 30.0%
ATGAATGGCAACAATTTAAG+AGG - chr4_2:66124870-66124889 Msa0616310:CDS 30.0%
TTTGGACAATATTTGTAGGT+AGG + chr4_2:66124924-66124943 None:intergenic 30.0%
CGAATTTGGACAATATTTGT+AGG + chr4_2:66124928-66124947 None:intergenic 30.0%
TTGTTAATTTCTTACCAAGC+TGG + chr4_2:66125005-66125024 None:intergenic 30.0%
TAGGACAATAAACAGAAATC+TGG - chr4_2:66125187-66125206 Msa0616310:intron 30.0%
ACAATAAACAGAAATCTGGT+TGG - chr4_2:66125191-66125210 Msa0616310:intron 30.0%
CACTTTATAAGAATTTCGTC+GGG - chr4_2:66125215-66125234 Msa0616310:intron 30.0%
TTAGGTGTGATAGAATAGTA+TGG - chr4_2:66125362-66125381 Msa0616310:intron 30.0%
TGTGATAGAATAGTATGGTA+TGG - chr4_2:66125367-66125386 Msa0616310:intron 30.0%
TAGAATAGTATGGTATGGAA+AGG - chr4_2:66125372-66125391 Msa0616310:intron 30.0%
AGAATAGTATGGTATGGAAA+GGG - chr4_2:66125373-66125392 Msa0616310:intron 30.0%
AATCAAGACTATCAGATCAT+AGG - chr4_2:66125425-66125444 Msa0616310:intron 30.0%
AGGATAATCTGCTATGAAGT+TGG + chr4_2:66122705-66122724 None:intergenic 35.0%
CTTGTATTTGTGTTTGTCCT+TGG + chr4_2:66122795-66122814 None:intergenic 35.0%
ATCTTCTGATCACTAGTACT+TGG - chr4_2:66122832-66122851 Msa0616310:CDS 35.0%
TTAGTACATGGAACATAGGT+GGG - chr4_2:66122930-66122949 Msa0616310:intron 35.0%
!! AAACGTAGATGATTTGTTGG+AGG - chr4_2:66122973-66122992 Msa0616310:intron 35.0%
CTATACAAAACATGGATCAG+TGG + chr4_2:66123041-66123060 None:intergenic 35.0%
ACATTGGAAATCATCATGTG+TGG + chr4_2:66123274-66123293 None:intergenic 35.0%
ACACTTGGATGTTGAATGAA+TGG + chr4_2:66123357-66123376 None:intergenic 35.0%
TGTGGCATAATGAGATGTTT+AGG - chr4_2:66123390-66123409 Msa0616310:intron 35.0%
TCACTTGTCAACATTCGATT+TGG - chr4_2:66123519-66123538 Msa0616310:CDS 35.0%
ATGTGTGTGATTGTTCTTAC+CGG + chr4_2:66123549-66123568 None:intergenic 35.0%
TCATGCAATAAGCACATTAG+TGG + chr4_2:66123688-66123707 None:intergenic 35.0%
!!! GACTATTGGCTGCTTTTAAA+AGG + chr4_2:66123756-66123775 None:intergenic 35.0%
!!! GATGCAAGGTTTTAGACTAT+TGG + chr4_2:66123770-66123789 None:intergenic 35.0%
GCAGAAACCTGCAAATTAAT+TGG + chr4_2:66123824-66123843 None:intergenic 35.0%
ACAGAACCCTGCAAATTAAT+TGG + chr4_2:66123913-66123932 None:intergenic 35.0%
AATTCAAACAAAGGACCTCT+AGG + chr4_2:66124066-66124085 None:intergenic 35.0%
!!! TTGGAGGCATTTTTTCTATG+GGG + chr4_2:66124313-66124332 None:intergenic 35.0%
!! AAAGTGTCCTATCTGTTTTC+TGG - chr4_2:66124395-66124414 Msa0616310:intron 35.0%
GTAACTTTCTATTTACGCAC+CGG + chr4_2:66124440-66124459 None:intergenic 35.0%
TGCAGTCATGTAATATTGCT+TGG - chr4_2:66124651-66124670 Msa0616310:intron 35.0%
TTATAGGATATCTCTTCCAG+AGG - chr4_2:66124683-66124702 Msa0616310:intron 35.0%
ACGCTTACTTGTAGAGAAAA+AGG - chr4_2:66124713-66124732 Msa0616310:intron 35.0%
TACTTGTAGAGAAAAAGGAC+TGG - chr4_2:66124718-66124737 Msa0616310:intron 35.0%
ACTTGTAGAGAAAAAGGACT+GGG - chr4_2:66124719-66124738 Msa0616310:intron 35.0%
! ATCATTAGCTTCATTCCCAT+TGG + chr4_2:66124843-66124862 None:intergenic 35.0%
AATGGGAATGAAGCTAATGA+TGG - chr4_2:66124842-66124861 Msa0616310:CDS 35.0%
ATGGCAACAATTTAAGAGGA+TGG - chr4_2:66124874-66124893 Msa0616310:CDS 35.0%
AATTTAAGAGGATGGCATGA+AGG - chr4_2:66124882-66124901 Msa0616310:CDS 35.0%
TAGGTAGGAGCAAATTTGAT+TGG + chr4_2:66124909-66124928 None:intergenic 35.0%
! TATTATGGTTGCTGTTGTCA+TGG - chr4_2:66124954-66124973 Msa0616310:CDS 35.0%
TGAAACAACTTGAGTACACA+AGG - chr4_2:66125398-66125417 Msa0616310:intron 35.0%
GCAGAAAAAATTGCCTTAAC+AGG + chr4_2:66125451-66125470 None:intergenic 35.0%
AGAAATAAGGGTTTCATCTG+AGG - chr4_2:66125471-66125490 Msa0616310:CDS 35.0%
TCTGTGGTAGAAACTTCAAA+AGG + chr4_2:66125550-66125569 None:intergenic 35.0%
TCTACATACAAGTTCATCTG+TGG + chr4_2:66125566-66125585 None:intergenic 35.0%
GTAGAAAACAATCAAGCTTC+CGG - chr4_2:66125581-66125600 Msa0616310:CDS 35.0%
AAGATACTCAAGAAGCGACT+AGG - chr4_2:66122674-66122693 Msa0616310:CDS 40.0%
AACAGTTCCTAATCATCCCA+AGG - chr4_2:66122775-66122794 Msa0616310:CDS 40.0%
TGTTTGTCCTTGGGATGATT+AGG + chr4_2:66122785-66122804 None:intergenic 40.0%
GTTAGTACATGGAACATAGG+TGG - chr4_2:66122929-66122948 Msa0616310:intron 40.0%
TCCTGTGACATCTATGTACT+TGG - chr4_2:66123007-66123026 Msa0616310:intron 40.0%
CCTGTGACATCTATGTACTT+GGG - chr4_2:66123008-66123027 Msa0616310:intron 40.0%
GAAACAAGGTGAGACCTATA+AGG - chr4_2:66123412-66123431 Msa0616310:intron 40.0%
!! CAACATTCGATTTGGATTGC+CGG - chr4_2:66123527-66123546 Msa0616310:CDS 40.0%
CAATGTAACAACCACTGATG+TGG + chr4_2:66123583-66123602 None:intergenic 40.0%
! TCTAAAACCTTGCATCCCAT+TGG - chr4_2:66123774-66123793 Msa0616310:intron 40.0%
! TTTTCTATGGGGACCAAAGT+TGG + chr4_2:66124302-66124321 None:intergenic 40.0%
TGTATGAGTCAGTGTCATGT+CGG - chr4_2:66125148-66125167 Msa0616310:intron 40.0%
AAGAATTTCGTCGGGAAATC+TGG - chr4_2:66125223-66125242 Msa0616310:intron 40.0%
ATCAGATCATAGGCCTGTTA+AGG - chr4_2:66125435-66125454 Msa0616310:intron 40.0%
CCCAAGTACATAGATGTCAC+AGG + chr4_2:66123011-66123030 None:intergenic 45.0%
CATCCAAGTGTGTCATGCAT+AGG - chr4_2:66123366-66123385 Msa0616310:CDS 45.0%
CAACCTATGCATGACACACT+TGG + chr4_2:66123372-66123391 None:intergenic 45.0%
AGTGTGTCATGCATAGGTTG+TGG - chr4_2:66123372-66123391 Msa0616310:CDS 45.0%
AAGGTCGACGTTGACGTATT+TGG + chr4_2:66123737-66123756 None:intergenic 45.0%
CAGTTAACCAATGGGATGCA+AGG + chr4_2:66123784-66123803 None:intergenic 45.0%
! TGTGTAGCCATCTAGCTTCA+GGG - chr4_2:66123974-66123993 Msa0616310:intron 45.0%
! AAGAAGGGGATGAGAAGTGT+AGG - chr4_2:66124001-66124020 Msa0616310:intron 45.0%
!! TCTAGGACTAGCTTTCCTAG+AGG - chr4_2:66124048-66124067 Msa0616310:intron 45.0%
ATTTCGTCGGGAAATCTGGT+TGG - chr4_2:66125227-66125246 Msa0616310:intron 45.0%
!! TTAATAATATTAATTTCATA+TGG + chr4_2:66123464-66123483 None:intergenic 5.0%
TCACGTAGCTACCACATCAG+TGG - chr4_2:66123569-66123588 Msa0616310:CDS 50.0%
! GTGTGTAGCCATCTAGCTTC+AGG - chr4_2:66123973-66123992 Msa0616310:intron 50.0%
! GTGTAGCCATCTAGCTTCAG+GGG - chr4_2:66123975-66123994 Msa0616310:intron 50.0%
! TGTAGCCATCTAGCTTCAGG+GGG - chr4_2:66123976-66123995 Msa0616310:intron 50.0%
TAGCTTCAGGGGGCAAAGAA+GGG - chr4_2:66123986-66124005 Msa0616310:intron 50.0%
!! AGTAAGCGTGTCGTTTCCTC+TGG + chr4_2:66124702-66124721 None:intergenic 50.0%
CGGAGTACACTGACACATGT+AGG - chr4_2:66125168-66125187 Msa0616310:intron 50.0%
CTTTGCCCCCTGAAGCTAGA+TGG + chr4_2:66123984-66124003 None:intergenic 55.0%
CTAGCTTCAGGGGGCAAAGA+AGG - chr4_2:66123985-66124004 Msa0616310:intron 55.0%
AGCTTCAGGGGGCAAAGAAG+GGG - chr4_2:66123987-66124006 Msa0616310:intron 55.0%
AAAAGAGGCGAGCACCAGCT+TGG - chr4_2:66124988-66125007 Msa0616310:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 66122650 66125609 66122650 ID=Msa0616310;Name=Msa0616310
chr4_2 mRNA 66122650 66125609 66122650 ID=Msa0616310-mRNA-1;Parent=Msa0616310;Name=Msa0616310-mRNA-1;_AED=0.08;_eAED=0.13;_QI=0|0|0|1|1|1|7|0|414
chr4_2 exon 66125446 66125609 66125446 ID=Msa0616310-mRNA-1:exon:18826;Parent=Msa0616310-mRNA-1
chr4_2 exon 66125230 66125337 66125230 ID=Msa0616310-mRNA-1:exon:18825;Parent=Msa0616310-mRNA-1
chr4_2 exon 66124840 66125119 66124840 ID=Msa0616310-mRNA-1:exon:18824;Parent=Msa0616310-mRNA-1
chr4_2 exon 66124150 66124334 66124150 ID=Msa0616310-mRNA-1:exon:18823;Parent=Msa0616310-mRNA-1
chr4_2 exon 66123495 66123603 66123495 ID=Msa0616310-mRNA-1:exon:18822;Parent=Msa0616310-mRNA-1
chr4_2 exon 66123250 66123404 66123250 ID=Msa0616310-mRNA-1:exon:18821;Parent=Msa0616310-mRNA-1
chr4_2 exon 66122650 66122893 66122650 ID=Msa0616310-mRNA-1:exon:18820;Parent=Msa0616310-mRNA-1
chr4_2 CDS 66125446 66125609 66125446 ID=Msa0616310-mRNA-1:cds;Parent=Msa0616310-mRNA-1
chr4_2 CDS 66125230 66125337 66125230 ID=Msa0616310-mRNA-1:cds;Parent=Msa0616310-mRNA-1
chr4_2 CDS 66124840 66125119 66124840 ID=Msa0616310-mRNA-1:cds;Parent=Msa0616310-mRNA-1
chr4_2 CDS 66124150 66124334 66124150 ID=Msa0616310-mRNA-1:cds;Parent=Msa0616310-mRNA-1
chr4_2 CDS 66123495 66123603 66123495 ID=Msa0616310-mRNA-1:cds;Parent=Msa0616310-mRNA-1
chr4_2 CDS 66123250 66123404 66123250 ID=Msa0616310-mRNA-1:cds;Parent=Msa0616310-mRNA-1
chr4_2 CDS 66122650 66122893 66122650 ID=Msa0616310-mRNA-1:cds;Parent=Msa0616310-mRNA-1
Gene Sequence

>Msa0616310

ATGTGGCCTACATTAGTGGCTAATAAGATACTCAAGAAGCGACTAGGAAGCACCAACTTCATAGCAGATTATCCTAGTTATGATGAACCTTTGTTGAATTTTGCAGACATTGATCATAGTTCTGAAACAGTTCCTAATCATCCCAAGGACAAACACAAATACAAGATTTTTGTTAGTACATGGAACATAGGTGGGATTGCACCAGATGAAGATTTAAACGTAGATGATTTGTTGGAGGCAAAAAACAACTCCTGTGACATCTATGTACTTGGGTTTCAAGAAATAGTGCCTCTAAAAGCTTCAAATGTATTGGGATCAGAAAACAATGAAATTTCCAATAAATGGAATTCCATAATCAGAAAAGCTTTGAATAAGGATCATAGAGACAAAGTTCATAGTGAATCCACACATGATGATTTCCAATGTATAATTAGCAAACAAATGGTTGGAATATTCATATCTGTATGGACAAGGAGAGATATTCGTCCATTCATTCAACATCCAAGTGTGTCATGCATAGGTTGTGGCATAATGAGATGTTTAGGAAACAAGGGTTCTGTATCAGTGAGATTTCAACTACATGAAGCAAGCTTCTGCTTTGTGTGTAGCCATCTAGCTTCAGGGGGCAAAGAAGGGGATGAGAAGTGTAGGAATTCTAATGTTGCTGAAATTTTTTCTAGGACTAGCTTTCCTAGAGGTCCTTTGTTTGAATTGCCAAGAAATATTCTAGACCATGATCATGTAATATTGCTTGGAGATTTAAATTATAGGATATCTCTTCCAGAGGAAACGACACGCTTACTTGTAGAGAAAAAGGACTGGGATTCCTTATTAGAAAATGATCAGCTAATGATGGAGTTAATGAATGGCAACAATTTAAGAGGATGGCATGAAGGACCAATCAAATTTGCTCCTACCTACAAATATTGTCCAAATTCGGATATATATTATGGTTGCTGTTGTCATGGAAAAAAGATAGAAAAGAGGCGAGCACCAGCTTGGTGTGATAGAATAGTATGGTATGGAAAGGGTTTGAAACAACTTGAGTACACAAGGAGTGAATCAAGACTATCAGATCATAGGCCTGTTAAGGCAATTTTTTCTGCAGAAATAAGGGTTTCATCTGAGGTCAAAAGCTTACAGAATTTGTCTCTATCAGAAAGATTTGAACAAATTAAAACTCCTTTTGAAGTTTCTACCACAGATGAACTTGTATGTAGAAAACAATCAAGCTTCCGGTTGTAA
Protein sequence

>Msa0616310

MWPTLVANKILKKRLGSTNFIADYPSYDEPLLNFADIDHSSETVPNHPKDKHKYKIFVSTWNIGGIAPDEDLNVDDLLEAKNNSCDIYVLGFQEIVPLKASNVLGSENNEISNKWNSIIRKALNKDHRDKVHSESTHDDFQCIISKQMVGIFISVWTRRDIRPFIQHPSVSCIGCGIMRCLGNKGSVSVRFQLHEASFCFVCSHLASGGKEGDEKCRNSNVAEIFSRTSFPRGPLFELPRNILDHDHVILLGDLNYRISLPEETTRLLVEKKDWDSLLENDQLMMELMNGNNLRGWHEGPIKFAPTYKYCPNSDIYYGCCCHGKKIEKRRAPAWCDRIVWYGKGLKQLEYTRSESRLSDHRPVKAIFSAEIRVSSEVKSLQNLSLSERFEQIKTPFEVSTTDELVCRKQSSFRL*