Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0616980 | Msa0697380 | 0.921472 | 3.423981e-88 | -8.615850e-47 |
| Msa0616980 | Msa0757030 | 0.895906 | 6.138818e-76 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0616980 | MtrunA17_Chr8g0386311 | 75.676 | 37 | 9 | 0 | 5 | 41 | 44 | 80 | 2.07e-11 | 56.6 |
| Msa0616980 | MtrunA17_Chr3g0132111 | 69.231 | 39 | 12 | 0 | 3 | 41 | 41 | 79 | 6.32e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATAGACCGTTGATGTTTC+AGG | 0.247801 | 4_2:-67272869 | None:intergenic |
| GTATCTGATGTGGTTTAGTT+TGG | 0.276776 | 4_2:+67273987 | Msa0616980:intron |
| TATCTGATGTGGTTTAGTTT+GGG | 0.288954 | 4_2:+67273988 | Msa0616980:three_prime_UTR |
| GCAACTTTCAAAGTCATTGA+TGG | 0.343752 | 4_2:+67272844 | Msa0616980:CDS |
| TTGAAAGTTGCAATGATGTC+AGG | 0.435539 | 4_2:-67272832 | None:intergenic |
| TTTGCAGTTAATATCCTTGA+GGG | 0.481204 | 4_2:+67273540 | Msa0616980:intron |
| CAATTTCTAACTGATTCAAA+TGG | 0.497571 | 4_2:+67272929 | Msa0616980:CDS |
| ATCTGATGTGGTTTAGTTTG+GGG | 0.497992 | 4_2:+67273989 | Msa0616980:three_prime_UTR |
| GTGCATCTAGGCGAGGGAAG+TGG | 0.538097 | 4_2:-67273574 | None:intergenic |
| TTCTTTGTTAGTATCTGATG+TGG | 0.569322 | 4_2:+67273977 | Msa0616980:intron |
| AACGTACAGTAGGTGCATCT+AGG | 0.603806 | 4_2:-67273586 | None:intergenic |
| TGGTTGTCTCATCACCCTCA+AGG | 0.606403 | 4_2:-67273554 | None:intergenic |
| CAGTAGGTGCATCTAGGCGA+GGG | 0.612296 | 4_2:-67273580 | None:intergenic |
| ACAGTAGGTGCATCTAGGCG+AGG | 0.637934 | 4_2:-67273581 | None:intergenic |
| GTTAGACATGATGAAAACAA+AGG | 0.640821 | 4_2:+67274045 | Msa0616980:three_prime_UTR |
| GATGGTGCCTGAAACATCAA+CGG | 0.665675 | 4_2:+67272862 | Msa0616980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAAAAATTATTTAAAATT+AGG | - | chr4_2:67273779-67273798 | None:intergenic | 0.0% |
| !!! | TTTAAATAATTTTTTTAATT+AGG | + | chr4_2:67273782-67273801 | Msa0616980:intron | 0.0% |
| !!! | TAAAAAAATTATTTAAAATT+AGG | - | chr4_2:67273779-67273798 | None:intergenic | 0.0% |
| !!! | TTTAAATAATTTTTTTAATT+AGG | + | chr4_2:67273782-67273801 | Msa0616980:intron | 0.0% |
| !! | TGGATTATTTAATTATGATT+TGG | + | chr4_2:67272979-67272998 | Msa0616980:intron | 15.0% |
| !! | TTTGTTTGTTTATTTGATAT+TGG | - | chr4_2:67273203-67273222 | None:intergenic | 15.0% |
| !! | TTAATTTAAAAGACAACAAA+GGG | - | chr4_2:67273253-67273272 | None:intergenic | 15.0% |
| !! | TGGATTATTTAATTATGATT+TGG | + | chr4_2:67272979-67272998 | Msa0616980:intron | 15.0% |
| !! | TTTGTTTGTTTATTTGATAT+TGG | - | chr4_2:67273203-67273222 | None:intergenic | 15.0% |
| !! | TTAATTTAAAAGACAACAAA+GGG | - | chr4_2:67273253-67273272 | None:intergenic | 15.0% |
| !! | GTTAATTTAAAAGACAACAA+AGG | - | chr4_2:67273254-67273273 | None:intergenic | 20.0% |
| !!! | ATTAGTCTATGAGTATAATT+GGG | - | chr4_2:67273410-67273429 | None:intergenic | 20.0% |
| !!! | AATTAGTCTATGAGTATAAT+TGG | - | chr4_2:67273411-67273430 | None:intergenic | 20.0% |
| !! | TATACTCATAGACTAATTTA+TGG | + | chr4_2:67273413-67273432 | Msa0616980:intron | 20.0% |
| !! | AGATACAATAAAACAGTAAA+AGG | - | chr4_2:67273900-67273919 | None:intergenic | 20.0% |
| !! | GTTAATTTAAAAGACAACAA+AGG | - | chr4_2:67273254-67273273 | None:intergenic | 20.0% |
| !!! | ATTAGTCTATGAGTATAATT+GGG | - | chr4_2:67273410-67273429 | None:intergenic | 20.0% |
| !!! | AATTAGTCTATGAGTATAAT+TGG | - | chr4_2:67273411-67273430 | None:intergenic | 20.0% |
| !! | TATACTCATAGACTAATTTA+TGG | + | chr4_2:67273413-67273432 | Msa0616980:intron | 20.0% |
| !! | AGATACAATAAAACAGTAAA+AGG | - | chr4_2:67273900-67273919 | None:intergenic | 20.0% |
| !! | CAATTTCTAACTGATTCAAA+TGG | + | chr4_2:67272929-67272948 | Msa0616980:CDS | 25.0% |
| ! | ATTTATGAACTTCTTCACTA+TGG | + | chr4_2:67272954-67272973 | Msa0616980:intron | 25.0% |
| ! | GCGAAGAAACATAATTAAAA+TGG | - | chr4_2:67273621-67273640 | None:intergenic | 25.0% |
| !! | ATGACAATTTTTGTATCATC+TGG | + | chr4_2:67273732-67273751 | Msa0616980:intron | 25.0% |
| ! | ATAGCTTATTCTATGACAAT+TGG | - | chr4_2:67273834-67273853 | None:intergenic | 25.0% |
| !! | CAATTTCTAACTGATTCAAA+TGG | + | chr4_2:67272929-67272948 | Msa0616980:CDS | 25.0% |
| ! | ATTTATGAACTTCTTCACTA+TGG | + | chr4_2:67272954-67272973 | Msa0616980:intron | 25.0% |
| ! | GCGAAGAAACATAATTAAAA+TGG | - | chr4_2:67273621-67273640 | None:intergenic | 25.0% |
| !! | ATGACAATTTTTGTATCATC+TGG | + | chr4_2:67273732-67273751 | Msa0616980:intron | 25.0% |
| ! | ATAGCTTATTCTATGACAAT+TGG | - | chr4_2:67273834-67273853 | None:intergenic | 25.0% |
| !! | TTGATATTGGTGCTAAATTC+AGG | - | chr4_2:67273190-67273209 | None:intergenic | 30.0% |
| AAAGACAACAAAGGGAAATT+TGG | - | chr4_2:67273245-67273264 | None:intergenic | 30.0% | |
| GATGATTAATTCGATCAGTT+TGG | + | chr4_2:67273294-67273313 | Msa0616980:intron | 30.0% | |
| ! | ATCAGTTTGGTAATATCTGT+TGG | + | chr4_2:67273307-67273326 | Msa0616980:intron | 30.0% |
| ! | TCAGTTTGGTAATATCTGTT+GGG | + | chr4_2:67273308-67273327 | Msa0616980:intron | 30.0% |
| ACTTATCATGAACATAAAGC+AGG | - | chr4_2:67273362-67273381 | None:intergenic | 30.0% | |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | - | chr4_2:67273447-67273466 | None:intergenic | 30.0% |
| ! | TTTTGCAGTTAATATCCTTG+AGG | + | chr4_2:67273539-67273558 | Msa0616980:intron | 30.0% |
| TTTGCAGTTAATATCCTTGA+GGG | + | chr4_2:67273540-67273559 | Msa0616980:intron | 30.0% | |
| TTCTATGACAATTGGCATAA+GGG | - | chr4_2:67273826-67273845 | None:intergenic | 30.0% | |
| ATTCTATGACAATTGGCATA+AGG | - | chr4_2:67273827-67273846 | None:intergenic | 30.0% | |
| TTCTTTGTTAGTATCTGATG+TGG | + | chr4_2:67273977-67273996 | Msa0616980:intron | 30.0% | |
| ! | TATCTGATGTGGTTTAGTTT+GGG | + | chr4_2:67273988-67274007 | Msa0616980:three_prime_UTR | 30.0% |
| GTTAGACATGATGAAAACAA+AGG | + | chr4_2:67274045-67274064 | Msa0616980:three_prime_UTR | 30.0% | |
| ! | ATGAAAACAAAGGCATGTAA+GGG | + | chr4_2:67274055-67274074 | Msa0616980:three_prime_UTR | 30.0% |
| !! | TTGATATTGGTGCTAAATTC+AGG | - | chr4_2:67273190-67273209 | None:intergenic | 30.0% |
| AAAGACAACAAAGGGAAATT+TGG | - | chr4_2:67273245-67273264 | None:intergenic | 30.0% | |
| GATGATTAATTCGATCAGTT+TGG | + | chr4_2:67273294-67273313 | Msa0616980:intron | 30.0% | |
| ! | ATCAGTTTGGTAATATCTGT+TGG | + | chr4_2:67273307-67273326 | Msa0616980:intron | 30.0% |
| ! | TCAGTTTGGTAATATCTGTT+GGG | + | chr4_2:67273308-67273327 | Msa0616980:intron | 30.0% |
| ACTTATCATGAACATAAAGC+AGG | - | chr4_2:67273362-67273381 | None:intergenic | 30.0% | |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | - | chr4_2:67273447-67273466 | None:intergenic | 30.0% |
| ! | TTTTGCAGTTAATATCCTTG+AGG | + | chr4_2:67273539-67273558 | Msa0616980:intron | 30.0% |
| TTTGCAGTTAATATCCTTGA+GGG | + | chr4_2:67273540-67273559 | Msa0616980:intron | 30.0% | |
| TTCTATGACAATTGGCATAA+GGG | - | chr4_2:67273826-67273845 | None:intergenic | 30.0% | |
| ATTCTATGACAATTGGCATA+AGG | - | chr4_2:67273827-67273846 | None:intergenic | 30.0% | |
| TTCTTTGTTAGTATCTGATG+TGG | + | chr4_2:67273977-67273996 | Msa0616980:intron | 30.0% | |
| ! | TATCTGATGTGGTTTAGTTT+GGG | + | chr4_2:67273988-67274007 | Msa0616980:three_prime_UTR | 30.0% |
| GTTAGACATGATGAAAACAA+AGG | + | chr4_2:67274045-67274064 | Msa0616980:three_prime_UTR | 30.0% | |
| TTGAAAGTTGCAATGATGTC+AGG | - | chr4_2:67272835-67272854 | None:intergenic | 35.0% | |
| GCAACTTTCAAAGTCATTGA+TGG | + | chr4_2:67272844-67272863 | Msa0616980:CDS | 35.0% | |
| ! | CTATGAAGTTTTTCGTCGAA+TGG | + | chr4_2:67272886-67272905 | Msa0616980:CDS | 35.0% |
| TGAACTTCTTCACTATGGAA+TGG | + | chr4_2:67272959-67272978 | Msa0616980:intron | 35.0% | |
| AACTTTGCTCGCTAATATCT+TGG | + | chr4_2:67273030-67273049 | Msa0616980:intron | 35.0% | |
| ACTTTGCTCGCTAATATCTT+GGG | + | chr4_2:67273031-67273050 | Msa0616980:intron | 35.0% | |
| TGGTTGTTTAGCCTCTTAAA+AGG | + | chr4_2:67273433-67273452 | Msa0616980:intron | 35.0% | |
| GAGAGAAAAAAACGTACAGT+AGG | - | chr4_2:67273599-67273618 | None:intergenic | 35.0% | |
| CACATCTATTCGAAATGCTT+GGG | - | chr4_2:67273674-67273693 | None:intergenic | 35.0% | |
| TCACATCTATTCGAAATGCT+TGG | - | chr4_2:67273675-67273694 | None:intergenic | 35.0% | |
| AGCATTTCGAATAGATGTGA+TGG | + | chr4_2:67273675-67273694 | Msa0616980:intron | 35.0% | |
| GCATTTCGAATAGATGTGAT+GGG | + | chr4_2:67273676-67273695 | Msa0616980:intron | 35.0% | |
| ! | AATAGATGTGATGGGACAAA+AGG | + | chr4_2:67273684-67273703 | Msa0616980:intron | 35.0% |
| GTATCTGATGTGGTTTAGTT+TGG | + | chr4_2:67273987-67274006 | Msa0616980:intron | 35.0% | |
| !! | ATCTGATGTGGTTTAGTTTG+GGG | + | chr4_2:67273989-67274008 | Msa0616980:three_prime_UTR | 35.0% |
| ! | GATGAAAACAAAGGCATGTA+AGG | + | chr4_2:67274054-67274073 | Msa0616980:three_prime_UTR | 35.0% |
| TTGAAAGTTGCAATGATGTC+AGG | - | chr4_2:67272835-67272854 | None:intergenic | 35.0% | |
| GCAACTTTCAAAGTCATTGA+TGG | + | chr4_2:67272844-67272863 | Msa0616980:CDS | 35.0% | |
| ! | CTATGAAGTTTTTCGTCGAA+TGG | + | chr4_2:67272886-67272905 | Msa0616980:CDS | 35.0% |
| TGAACTTCTTCACTATGGAA+TGG | + | chr4_2:67272959-67272978 | Msa0616980:intron | 35.0% | |
| AACTTTGCTCGCTAATATCT+TGG | + | chr4_2:67273030-67273049 | Msa0616980:intron | 35.0% | |
| ACTTTGCTCGCTAATATCTT+GGG | + | chr4_2:67273031-67273050 | Msa0616980:intron | 35.0% | |
| TGGTTGTTTAGCCTCTTAAA+AGG | + | chr4_2:67273433-67273452 | Msa0616980:intron | 35.0% | |
| GAGAGAAAAAAACGTACAGT+AGG | - | chr4_2:67273599-67273618 | None:intergenic | 35.0% | |
| CACATCTATTCGAAATGCTT+GGG | - | chr4_2:67273674-67273693 | None:intergenic | 35.0% | |
| TCACATCTATTCGAAATGCT+TGG | - | chr4_2:67273675-67273694 | None:intergenic | 35.0% | |
| AGCATTTCGAATAGATGTGA+TGG | + | chr4_2:67273675-67273694 | Msa0616980:intron | 35.0% | |
| GCATTTCGAATAGATGTGAT+GGG | + | chr4_2:67273676-67273695 | Msa0616980:intron | 35.0% | |
| ! | AATAGATGTGATGGGACAAA+AGG | + | chr4_2:67273684-67273703 | Msa0616980:intron | 35.0% |
| GTATCTGATGTGGTTTAGTT+TGG | + | chr4_2:67273987-67274006 | Msa0616980:intron | 35.0% | |
| !! | ATCTGATGTGGTTTAGTTTG+GGG | + | chr4_2:67273989-67274008 | Msa0616980:three_prime_UTR | 35.0% |
| TCATAGACCGTTGATGTTTC+AGG | - | chr4_2:67272872-67272891 | None:intergenic | 40.0% | |
| ! | CTGCTAGAAGCAACAGTTTT+AGG | + | chr4_2:67274014-67274033 | Msa0616980:three_prime_UTR | 40.0% |
| TCATAGACCGTTGATGTTTC+AGG | - | chr4_2:67272872-67272891 | None:intergenic | 40.0% | |
| ! | CTGCTAGAAGCAACAGTTTT+AGG | + | chr4_2:67274014-67274033 | Msa0616980:three_prime_UTR | 40.0% |
| GATGGTGCCTGAAACATCAA+CGG | + | chr4_2:67272862-67272881 | Msa0616980:CDS | 45.0% | |
| AACGTACAGTAGGTGCATCT+AGG | - | chr4_2:67273589-67273608 | None:intergenic | 45.0% | |
| GATGGTGCCTGAAACATCAA+CGG | + | chr4_2:67272862-67272881 | Msa0616980:CDS | 45.0% | |
| AACGTACAGTAGGTGCATCT+AGG | - | chr4_2:67273589-67273608 | None:intergenic | 45.0% | |
| TGGTTGTCTCATCACCCTCA+AGG | - | chr4_2:67273557-67273576 | None:intergenic | 50.0% | |
| TGGTTGTCTCATCACCCTCA+AGG | - | chr4_2:67273557-67273576 | None:intergenic | 50.0% | |
| CAGTAGGTGCATCTAGGCGA+GGG | - | chr4_2:67273583-67273602 | None:intergenic | 55.0% | |
| ACAGTAGGTGCATCTAGGCG+AGG | - | chr4_2:67273584-67273603 | None:intergenic | 55.0% | |
| CAGTAGGTGCATCTAGGCGA+GGG | - | chr4_2:67273583-67273602 | None:intergenic | 55.0% | |
| ACAGTAGGTGCATCTAGGCG+AGG | - | chr4_2:67273584-67273603 | None:intergenic | 55.0% | |
| GTGCATCTAGGCGAGGGAAG+TGG | - | chr4_2:67273577-67273596 | None:intergenic | 60.0% | |
| GTGCATCTAGGCGAGGGAAG+TGG | - | chr4_2:67273577-67273596 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 67272821 | 67274074 | 67272821 | ID=Msa0616980;Name=Msa0616980 |
| chr4_2 | mRNA | 67272821 | 67274074 | 67272821 | ID=Msa0616980-mRNA-1;Parent=Msa0616980;Name=Msa0616980-mRNA-1;_AED=0.00;_eAED=0.00;_QI=6|1|1|1|0.5|0.33|3|98|55 |
| chr4_2 | exon | 67272821 | 67272950 | 67272821 | ID=Msa0616980-mRNA-1:exon:19154;Parent=Msa0616980-mRNA-1 |
| chr4_2 | exon | 67273547 | 67273601 | 67273547 | ID=Msa0616980-mRNA-1:exon:19155;Parent=Msa0616980-mRNA-1 |
| chr4_2 | exon | 67273988 | 67274074 | 67273988 | ID=Msa0616980-mRNA-1:exon:19156;Parent=Msa0616980-mRNA-1 |
| chr4_2 | five_prime_UTR | 67272821 | 67272826 | 67272821 | ID=Msa0616980-mRNA-1:five_prime_utr;Parent=Msa0616980-mRNA-1 |
| chr4_2 | CDS | 67272827 | 67272950 | 67272827 | ID=Msa0616980-mRNA-1:cds;Parent=Msa0616980-mRNA-1 |
| chr4_2 | CDS | 67273547 | 67273590 | 67273547 | ID=Msa0616980-mRNA-1:cds;Parent=Msa0616980-mRNA-1 |
| chr4_2 | three_prime_UTR | 67273591 | 67273601 | 67273591 | ID=Msa0616980-mRNA-1:three_prime_utr;Parent=Msa0616980-mRNA-1 |
| chr4_2 | three_prime_UTR | 67273988 | 67274074 | 67273988 | ID=Msa0616980-mRNA-1:three_prime_utr;Parent=Msa0616980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
