Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0616980 | Msa0757030 | 0.895906 | 6.138818e-76 | -8.615850e-47 |
| Msa0697380 | Msa0757030 | 0.960118 | 3.381391e-118 | -8.615850e-47 |
| Msa0757030 | Msa0800390 | 0.887879 | 9.675467e-73 | -8.615850e-47 |
| Msa0757030 | Msa0962310 | 0.803480 | 3.416144e-49 | -8.615850e-47 |
| Msa0423920 | Msa0757030 | 0.805629 | 1.217157e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0757030 | MtrunA17_Chr8g0386311 | 75.676 | 37 | 9 | 0 | 5 | 41 | 44 | 80 | 2.07e-11 | 56.6 |
| Msa0757030 | MtrunA17_Chr3g0132111 | 69.231 | 39 | 12 | 0 | 3 | 41 | 41 | 79 | 6.32e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATAGACCGTTGATGTTTC+AGG | 0.247801 | 5_2:+11712298 | None:intergenic |
| GTATCTGATGTGGTTTAGTT+TGG | 0.276776 | 5_2:-11711180 | Msa0757030:three_prime_UTR |
| TATCTGATGTGGTTTAGTTT+GGG | 0.288954 | 5_2:-11711179 | Msa0757030:three_prime_UTR |
| GCAACTTTCAAAGTCATTGA+TGG | 0.343752 | 5_2:-11712323 | Msa0757030:CDS |
| TTGAAAGTTGCAATGATGTC+AGG | 0.435539 | 5_2:+11712335 | None:intergenic |
| TTTGCAGTTAATATCCTTGA+GGG | 0.481204 | 5_2:-11711627 | Msa0757030:intron |
| CAATTTCTAACTGATTCAAA+TGG | 0.497571 | 5_2:-11712238 | Msa0757030:intron |
| ATCTGATGTGGTTTAGTTTG+GGG | 0.497992 | 5_2:-11711178 | Msa0757030:three_prime_UTR |
| GTGAATCTAGGCGAGGGAAG+TGG | 0.550537 | 5_2:+11711593 | None:intergenic |
| AACGTACAGTAGGTGAATCT+AGG | 0.553854 | 5_2:+11711581 | None:intergenic |
| TTCTTTGTTAGTATCTGATG+TGG | 0.569322 | 5_2:-11711190 | Msa0757030:intron |
| TGGTTGTCTCATCACCCTCA+AGG | 0.606403 | 5_2:+11711613 | None:intergenic |
| CAGTAGGTGAATCTAGGCGA+GGG | 0.620624 | 5_2:+11711587 | None:intergenic |
| CTAGAAGCAACAGTTCTGCA+AGG | 0.631238 | 5_2:-11711150 | Msa0757030:three_prime_UTR |
| GTTAGACATGATGAAAACAA+AGG | 0.640821 | 5_2:-11711119 | Msa0757030:three_prime_UTR |
| ACAGTAGGTGAATCTAGGCG+AGG | 0.644049 | 5_2:+11711586 | None:intergenic |
| GATGGTGCCTGAAACATCAA+CGG | 0.665675 | 5_2:-11712305 | Msa0757030:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAAAATTTATTTAAAATT+AGG | + | chr5_2:11712070-11712089 | None:intergenic | 0.0% |
| !!! | TTTAAATAAATTTTTTAATT+AGG | - | chr5_2:11712073-11712092 | Msa0757030:intron | 0.0% |
| !!! | TAAAAAATTTATTTAAAATT+AGG | + | chr5_2:11712070-11712089 | None:intergenic | 0.0% |
| !!! | TTTAAATAAATTTTTTAATT+AGG | - | chr5_2:11712073-11712092 | Msa0757030:intron | 0.0% |
| !! | TGGATTATTTAATTATGATT+TGG | - | chr5_2:11711270-11711289 | Msa0757030:intron | 15.0% |
| !! | TTTGTTTGTTTATTTGATAT+TGG | + | chr5_2:11711494-11711513 | None:intergenic | 15.0% |
| !! | TTAATTTAAAAGACAACAAA+GGG | + | chr5_2:11711544-11711563 | None:intergenic | 15.0% |
| !! | TGGATTATTTAATTATGATT+TGG | - | chr5_2:11711270-11711289 | Msa0757030:intron | 15.0% |
| !! | TTTGTTTGTTTATTTGATAT+TGG | + | chr5_2:11711494-11711513 | None:intergenic | 15.0% |
| !! | TTAATTTAAAAGACAACAAA+GGG | + | chr5_2:11711544-11711563 | None:intergenic | 15.0% |
| !! | GTTAATTTAAAAGACAACAA+AGG | + | chr5_2:11711545-11711564 | None:intergenic | 20.0% |
| !!! | ATTAGTCTATGAGTATAATT+GGG | + | chr5_2:11711701-11711720 | None:intergenic | 20.0% |
| !!! | AATTAGTCTATGAGTATAAT+TGG | + | chr5_2:11711702-11711721 | None:intergenic | 20.0% |
| !! | TATACTCATAGACTAATTTA+TGG | - | chr5_2:11711704-11711723 | Msa0757030:intron | 20.0% |
| !! | AGATACAATAAAACAGTAAA+AGG | + | chr5_2:11712191-11712210 | None:intergenic | 20.0% |
| !! | GTTAATTTAAAAGACAACAA+AGG | + | chr5_2:11711545-11711564 | None:intergenic | 20.0% |
| !!! | ATTAGTCTATGAGTATAATT+GGG | + | chr5_2:11711701-11711720 | None:intergenic | 20.0% |
| !!! | AATTAGTCTATGAGTATAAT+TGG | + | chr5_2:11711702-11711721 | None:intergenic | 20.0% |
| !! | TATACTCATAGACTAATTTA+TGG | - | chr5_2:11711704-11711723 | Msa0757030:intron | 20.0% |
| !! | AGATACAATAAAACAGTAAA+AGG | + | chr5_2:11712191-11712210 | None:intergenic | 20.0% |
| !! | CAATTTCTAACTGATTCAAA+TGG | - | chr5_2:11711220-11711239 | Msa0757030:intron | 25.0% |
| ! | ATTTATGAACTTCTTCACTA+TGG | - | chr5_2:11711245-11711264 | Msa0757030:intron | 25.0% |
| ! | GCGAAGAAACATAATTAAAA+TGG | + | chr5_2:11711912-11711931 | None:intergenic | 25.0% |
| !! | ATGACAATTTTTGTATCATC+TGG | - | chr5_2:11712023-11712042 | Msa0757030:intron | 25.0% |
| ! | ATAGCTTATTCTATGACAAT+TGG | + | chr5_2:11712125-11712144 | None:intergenic | 25.0% |
| !! | CAATTTCTAACTGATTCAAA+TGG | - | chr5_2:11711220-11711239 | Msa0757030:intron | 25.0% |
| ! | ATTTATGAACTTCTTCACTA+TGG | - | chr5_2:11711245-11711264 | Msa0757030:intron | 25.0% |
| ! | GCGAAGAAACATAATTAAAA+TGG | + | chr5_2:11711912-11711931 | None:intergenic | 25.0% |
| !! | ATGACAATTTTTGTATCATC+TGG | - | chr5_2:11712023-11712042 | Msa0757030:intron | 25.0% |
| ! | ATAGCTTATTCTATGACAAT+TGG | + | chr5_2:11712125-11712144 | None:intergenic | 25.0% |
| !! | TTGATATTGGTGCTAAATTC+AGG | + | chr5_2:11711481-11711500 | None:intergenic | 30.0% |
| AAAGACAACAAAGGGAAATT+TGG | + | chr5_2:11711536-11711555 | None:intergenic | 30.0% | |
| GATGATTAATTCGATCAGTT+TGG | - | chr5_2:11711585-11711604 | Msa0757030:intron | 30.0% | |
| ! | ATCAGTTTGGTAATATCTGT+TGG | - | chr5_2:11711598-11711617 | Msa0757030:exon | 30.0% |
| ! | TCAGTTTGGTAATATCTGTT+GGG | - | chr5_2:11711599-11711618 | Msa0757030:CDS | 30.0% |
| ACTTATCATGAACATAAAGC+AGG | + | chr5_2:11711653-11711672 | None:intergenic | 30.0% | |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | + | chr5_2:11711738-11711757 | None:intergenic | 30.0% |
| ! | TTTTGCAGTTAATATCCTTG+AGG | - | chr5_2:11711830-11711849 | Msa0757030:intron | 30.0% |
| TTTGCAGTTAATATCCTTGA+GGG | - | chr5_2:11711831-11711850 | Msa0757030:intron | 30.0% | |
| TTCTATGACAATTGGCATAA+GGG | + | chr5_2:11712117-11712136 | None:intergenic | 30.0% | |
| ATTCTATGACAATTGGCATA+AGG | + | chr5_2:11712118-11712137 | None:intergenic | 30.0% | |
| TTCTTTGTTAGTATCTGATG+TGG | - | chr5_2:11712268-11712287 | Msa0757030:CDS | 30.0% | |
| ! | TATCTGATGTGGTTTAGTTT+GGG | - | chr5_2:11712279-11712298 | Msa0757030:CDS | 30.0% |
| GTTAGACATGATGAAAACAA+AGG | - | chr5_2:11712339-11712358 | Msa0757030:CDS | 30.0% | |
| ! | ATGAAAACAAAGGCATGTAA+GGG | - | chr5_2:11712349-11712368 | Msa0757030:exon | 30.0% |
| !! | TTGATATTGGTGCTAAATTC+AGG | + | chr5_2:11711481-11711500 | None:intergenic | 30.0% |
| AAAGACAACAAAGGGAAATT+TGG | + | chr5_2:11711536-11711555 | None:intergenic | 30.0% | |
| GATGATTAATTCGATCAGTT+TGG | - | chr5_2:11711585-11711604 | Msa0757030:intron | 30.0% | |
| ! | ATCAGTTTGGTAATATCTGT+TGG | - | chr5_2:11711598-11711617 | Msa0757030:exon | 30.0% |
| ! | TCAGTTTGGTAATATCTGTT+GGG | - | chr5_2:11711599-11711618 | Msa0757030:CDS | 30.0% |
| ACTTATCATGAACATAAAGC+AGG | + | chr5_2:11711653-11711672 | None:intergenic | 30.0% | |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | + | chr5_2:11711738-11711757 | None:intergenic | 30.0% |
| ! | TTTTGCAGTTAATATCCTTG+AGG | - | chr5_2:11711830-11711849 | Msa0757030:intron | 30.0% |
| TTTGCAGTTAATATCCTTGA+GGG | - | chr5_2:11711831-11711850 | Msa0757030:intron | 30.0% | |
| TTCTATGACAATTGGCATAA+GGG | + | chr5_2:11712117-11712136 | None:intergenic | 30.0% | |
| ATTCTATGACAATTGGCATA+AGG | + | chr5_2:11712118-11712137 | None:intergenic | 30.0% | |
| TTCTTTGTTAGTATCTGATG+TGG | - | chr5_2:11712268-11712287 | Msa0757030:CDS | 30.0% | |
| ! | TATCTGATGTGGTTTAGTTT+GGG | - | chr5_2:11712279-11712298 | Msa0757030:CDS | 30.0% |
| GTTAGACATGATGAAAACAA+AGG | - | chr5_2:11712339-11712358 | Msa0757030:CDS | 30.0% | |
| TTGAAAGTTGCAATGATGTC+AGG | + | chr5_2:11711126-11711145 | None:intergenic | 35.0% | |
| GCAACTTTCAAAGTCATTGA+TGG | - | chr5_2:11711135-11711154 | Msa0757030:three_prime_UTR | 35.0% | |
| ! | CTATGAAGTTTTTCGTCGAA+TGG | - | chr5_2:11711177-11711196 | Msa0757030:three_prime_UTR | 35.0% |
| TGAACTTCTTCACTATGGAA+TGG | - | chr5_2:11711250-11711269 | Msa0757030:intron | 35.0% | |
| AACTTTGCTCGCTAATATCT+TGG | - | chr5_2:11711321-11711340 | Msa0757030:intron | 35.0% | |
| ACTTTGCTCGCTAATATCTT+GGG | - | chr5_2:11711322-11711341 | Msa0757030:intron | 35.0% | |
| TGGTTGTTTAGCCTCTTAAA+AGG | - | chr5_2:11711724-11711743 | Msa0757030:intron | 35.0% | |
| GAGAGAAAAAAACGTACAGT+AGG | + | chr5_2:11711890-11711909 | None:intergenic | 35.0% | |
| CACATCTATTCGAAATGCTT+GGG | + | chr5_2:11711965-11711984 | None:intergenic | 35.0% | |
| TCACATCTATTCGAAATGCT+TGG | + | chr5_2:11711966-11711985 | None:intergenic | 35.0% | |
| AGCATTTCGAATAGATGTGA+TGG | - | chr5_2:11711966-11711985 | Msa0757030:intron | 35.0% | |
| GCATTTCGAATAGATGTGAT+GGG | - | chr5_2:11711967-11711986 | Msa0757030:intron | 35.0% | |
| ! | AATAGATGTGATGGGACAAA+AGG | - | chr5_2:11711975-11711994 | Msa0757030:intron | 35.0% |
| GTATCTGATGTGGTTTAGTT+TGG | - | chr5_2:11712278-11712297 | Msa0757030:CDS | 35.0% | |
| !! | ATCTGATGTGGTTTAGTTTG+GGG | - | chr5_2:11712280-11712299 | Msa0757030:CDS | 35.0% |
| ! | GATGAAAACAAAGGCATGTA+AGG | - | chr5_2:11712348-11712367 | Msa0757030:exon | 35.0% |
| TTGAAAGTTGCAATGATGTC+AGG | + | chr5_2:11711126-11711145 | None:intergenic | 35.0% | |
| GCAACTTTCAAAGTCATTGA+TGG | - | chr5_2:11711135-11711154 | Msa0757030:three_prime_UTR | 35.0% | |
| ! | CTATGAAGTTTTTCGTCGAA+TGG | - | chr5_2:11711177-11711196 | Msa0757030:three_prime_UTR | 35.0% |
| TGAACTTCTTCACTATGGAA+TGG | - | chr5_2:11711250-11711269 | Msa0757030:intron | 35.0% | |
| AACTTTGCTCGCTAATATCT+TGG | - | chr5_2:11711321-11711340 | Msa0757030:intron | 35.0% | |
| ACTTTGCTCGCTAATATCTT+GGG | - | chr5_2:11711322-11711341 | Msa0757030:intron | 35.0% | |
| TGGTTGTTTAGCCTCTTAAA+AGG | - | chr5_2:11711724-11711743 | Msa0757030:intron | 35.0% | |
| GAGAGAAAAAAACGTACAGT+AGG | + | chr5_2:11711890-11711909 | None:intergenic | 35.0% | |
| CACATCTATTCGAAATGCTT+GGG | + | chr5_2:11711965-11711984 | None:intergenic | 35.0% | |
| TCACATCTATTCGAAATGCT+TGG | + | chr5_2:11711966-11711985 | None:intergenic | 35.0% | |
| AGCATTTCGAATAGATGTGA+TGG | - | chr5_2:11711966-11711985 | Msa0757030:intron | 35.0% | |
| GCATTTCGAATAGATGTGAT+GGG | - | chr5_2:11711967-11711986 | Msa0757030:intron | 35.0% | |
| ! | AATAGATGTGATGGGACAAA+AGG | - | chr5_2:11711975-11711994 | Msa0757030:intron | 35.0% |
| GTATCTGATGTGGTTTAGTT+TGG | - | chr5_2:11712278-11712297 | Msa0757030:CDS | 35.0% | |
| !! | ATCTGATGTGGTTTAGTTTG+GGG | - | chr5_2:11712280-11712299 | Msa0757030:CDS | 35.0% |
| TCATAGACCGTTGATGTTTC+AGG | + | chr5_2:11711163-11711182 | None:intergenic | 40.0% | |
| AACGTACAGTAGGTGAATCT+AGG | + | chr5_2:11711880-11711899 | None:intergenic | 40.0% | |
| TCATAGACCGTTGATGTTTC+AGG | + | chr5_2:11711163-11711182 | None:intergenic | 40.0% | |
| AACGTACAGTAGGTGAATCT+AGG | + | chr5_2:11711880-11711899 | None:intergenic | 40.0% | |
| GATGGTGCCTGAAACATCAA+CGG | - | chr5_2:11711153-11711172 | Msa0757030:three_prime_UTR | 45.0% | |
| CTAGAAGCAACAGTTCTGCA+AGG | - | chr5_2:11712308-11712327 | Msa0757030:CDS | 45.0% | |
| GATGGTGCCTGAAACATCAA+CGG | - | chr5_2:11711153-11711172 | Msa0757030:three_prime_UTR | 45.0% | |
| CTAGAAGCAACAGTTCTGCA+AGG | - | chr5_2:11712308-11712327 | Msa0757030:CDS | 45.0% | |
| TGGTTGTCTCATCACCCTCA+AGG | + | chr5_2:11711848-11711867 | None:intergenic | 50.0% | |
| CAGTAGGTGAATCTAGGCGA+GGG | + | chr5_2:11711874-11711893 | None:intergenic | 50.0% | |
| ACAGTAGGTGAATCTAGGCG+AGG | + | chr5_2:11711875-11711894 | None:intergenic | 50.0% | |
| TGGTTGTCTCATCACCCTCA+AGG | + | chr5_2:11711848-11711867 | None:intergenic | 50.0% | |
| CAGTAGGTGAATCTAGGCGA+GGG | + | chr5_2:11711874-11711893 | None:intergenic | 50.0% | |
| ACAGTAGGTGAATCTAGGCG+AGG | + | chr5_2:11711875-11711894 | None:intergenic | 50.0% | |
| GTGAATCTAGGCGAGGGAAG+TGG | + | chr5_2:11711868-11711887 | None:intergenic | 55.0% | |
| GTGAATCTAGGCGAGGGAAG+TGG | + | chr5_2:11711868-11711887 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 11711112 | 11712368 | 11711112 | ID=Msa0757030;Name=Msa0757030 |
| chr5_2 | mRNA | 11711112 | 11712368 | 11711112 | ID=Msa0757030-mRNA-1;Parent=Msa0757030;Name=Msa0757030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=6|1|1|1|0.5|0.33|3|101|55 |
| chr5_2 | exon | 11711112 | 11711201 | 11711112 | ID=Msa0757030-mRNA-1:exon:5288;Parent=Msa0757030-mRNA-1 |
| chr5_2 | exon | 11711588 | 11711642 | 11711588 | ID=Msa0757030-mRNA-1:exon:5287;Parent=Msa0757030-mRNA-1 |
| chr5_2 | exon | 11712239 | 11712368 | 11712239 | ID=Msa0757030-mRNA-1:exon:5286;Parent=Msa0757030-mRNA-1 |
| chr5_2 | five_prime_UTR | 11712363 | 11712368 | 11712363 | ID=Msa0757030-mRNA-1:five_prime_utr;Parent=Msa0757030-mRNA-1 |
| chr5_2 | CDS | 11712239 | 11712362 | 11712239 | ID=Msa0757030-mRNA-1:cds;Parent=Msa0757030-mRNA-1 |
| chr5_2 | CDS | 11711599 | 11711642 | 11711599 | ID=Msa0757030-mRNA-1:cds;Parent=Msa0757030-mRNA-1 |
| chr5_2 | three_prime_UTR | 11711588 | 11711598 | 11711588 | ID=Msa0757030-mRNA-1:three_prime_utr;Parent=Msa0757030-mRNA-1 |
| chr5_2 | three_prime_UTR | 11711112 | 11711201 | 11711112 | ID=Msa0757030-mRNA-1:three_prime_utr;Parent=Msa0757030-mRNA-1 |
| Gene Sequence |
| Protein sequence |
