Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0616980 | Msa0697380 | 0.921472 | 3.423981e-88 | -8.615850e-47 |
| Msa0697380 | Msa0757030 | 0.960118 | 3.381391e-118 | -8.615850e-47 |
| Msa0697380 | Msa0800390 | 0.884417 | 1.952226e-71 | -8.615850e-47 |
| Msa0697380 | Msa0962310 | 0.826459 | 2.682259e-54 | -8.615850e-47 |
| Msa0423920 | Msa0697380 | 0.832698 | 8.149424e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0697380 | MtrunA17_Chr8g0386311 | 75.676 | 37 | 9 | 0 | 5 | 41 | 44 | 80 | 2.07e-11 | 56.6 |
| Msa0697380 | MtrunA17_Chr3g0132111 | 69.231 | 39 | 12 | 0 | 3 | 41 | 41 | 79 | 6.32e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATAGACCGTTGATGTTTC+AGG | 0.247801 | 4_4:-67381158 | None:intergenic |
| GTATCTGATGTGGTTTAGTT+TGG | 0.276776 | 4_4:+67382276 | Msa0697380:intron |
| TATCTGATGTGGTTTAGTTT+GGG | 0.288954 | 4_4:+67382277 | Msa0697380:three_prime_UTR |
| GCAACTTTCAAAGTCATTGA+TGG | 0.343752 | 4_4:+67381133 | Msa0697380:CDS |
| TTGAAAGTTGCAATGATGTC+AGG | 0.435539 | 4_4:-67381121 | None:intergenic |
| TTTGCAGTTAATATCCTTGA+GGG | 0.481204 | 4_4:+67381829 | Msa0697380:intron |
| CAATTTCTAACTGATTCAAA+TGG | 0.497571 | 4_4:+67381218 | Msa0697380:CDS |
| ATCTGATGTGGTTTAGTTTG+GGG | 0.497992 | 4_4:+67382278 | Msa0697380:three_prime_UTR |
| GTGAATCTAGGCGAGGGAAG+TGG | 0.550537 | 4_4:-67381863 | None:intergenic |
| AACGTACAGTAGGTGAATCT+AGG | 0.553854 | 4_4:-67381875 | None:intergenic |
| TTCTTTGTTAGTATCTGATG+TGG | 0.569322 | 4_4:+67382266 | Msa0697380:intron |
| TGGTTGTCTCATCACCCTCA+AGG | 0.606403 | 4_4:-67381843 | None:intergenic |
| CAGTAGGTGAATCTAGGCGA+GGG | 0.620624 | 4_4:-67381869 | None:intergenic |
| CTAGAAGCAACAGTTCTGCA+AGG | 0.631238 | 4_4:+67382306 | Msa0697380:three_prime_UTR |
| GTTAGACATGATGAAAACAA+AGG | 0.640821 | 4_4:+67382337 | Msa0697380:three_prime_UTR |
| ACAGTAGGTGAATCTAGGCG+AGG | 0.644049 | 4_4:-67381870 | None:intergenic |
| GATGGTGCCTGAAACATCAA+CGG | 0.665675 | 4_4:+67381151 | Msa0697380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAAAATTTATTTAAAATT+AGG | - | chr4_4:67382068-67382087 | None:intergenic | 0.0% |
| !!! | TTTAAATAAATTTTTTAATT+AGG | + | chr4_4:67382071-67382090 | Msa0697380:intron | 0.0% |
| !!! | TAAAAAATTTATTTAAAATT+AGG | - | chr4_4:67382068-67382087 | None:intergenic | 0.0% |
| !!! | TTTAAATAAATTTTTTAATT+AGG | + | chr4_4:67382071-67382090 | Msa0697380:intron | 0.0% |
| !! | TTTGTTTGTTTATTTGATAT+TGG | - | chr4_4:67381492-67381511 | None:intergenic | 15.0% |
| !! | TTAATTTAAAAGACAACAAA+GGG | - | chr4_4:67381542-67381561 | None:intergenic | 15.0% |
| !! | AGATACAATAAAATAGTAAA+AGG | - | chr4_4:67382189-67382208 | None:intergenic | 15.0% |
| !! | TTTGTTTGTTTATTTGATAT+TGG | - | chr4_4:67381492-67381511 | None:intergenic | 15.0% |
| !! | TTAATTTAAAAGACAACAAA+GGG | - | chr4_4:67381542-67381561 | None:intergenic | 15.0% |
| !! | AGATACAATAAAATAGTAAA+AGG | - | chr4_4:67382189-67382208 | None:intergenic | 15.0% |
| !! | TGGATTATTTAATTGTGATT+TGG | + | chr4_4:67381268-67381287 | Msa0697380:intron | 20.0% |
| !! | GTTAATTTAAAAGACAACAA+AGG | - | chr4_4:67381543-67381562 | None:intergenic | 20.0% |
| !!! | ATTAGTCTATGAGTATAATT+GGG | - | chr4_4:67381699-67381718 | None:intergenic | 20.0% |
| !!! | AATTAGTCTATGAGTATAAT+TGG | - | chr4_4:67381700-67381719 | None:intergenic | 20.0% |
| !! | TATACTCATAGACTAATTTA+TGG | + | chr4_4:67381702-67381721 | Msa0697380:intron | 20.0% |
| !! | TGGATTATTTAATTGTGATT+TGG | + | chr4_4:67381268-67381287 | Msa0697380:intron | 20.0% |
| !! | GTTAATTTAAAAGACAACAA+AGG | - | chr4_4:67381543-67381562 | None:intergenic | 20.0% |
| !!! | ATTAGTCTATGAGTATAATT+GGG | - | chr4_4:67381699-67381718 | None:intergenic | 20.0% |
| !!! | AATTAGTCTATGAGTATAAT+TGG | - | chr4_4:67381700-67381719 | None:intergenic | 20.0% |
| !! | TATACTCATAGACTAATTTA+TGG | + | chr4_4:67381702-67381721 | Msa0697380:intron | 20.0% |
| !! | CAATTTCTAACTGATTCAAA+TGG | + | chr4_4:67381218-67381237 | Msa0697380:CDS | 25.0% |
| ! | ATTTATGAACTTCTTCACTA+TGG | + | chr4_4:67381243-67381262 | Msa0697380:intron | 25.0% |
| ! | GCGAAGAAACATAATTAAAA+TGG | - | chr4_4:67381910-67381929 | None:intergenic | 25.0% |
| !! | ATGACAATTTTTGTATCATC+TGG | + | chr4_4:67382021-67382040 | Msa0697380:intron | 25.0% |
| ! | ATAGCTTATTCTATGACAAT+TGG | - | chr4_4:67382123-67382142 | None:intergenic | 25.0% |
| !! | CAATTTCTAACTGATTCAAA+TGG | + | chr4_4:67381218-67381237 | Msa0697380:CDS | 25.0% |
| ! | ATTTATGAACTTCTTCACTA+TGG | + | chr4_4:67381243-67381262 | Msa0697380:intron | 25.0% |
| ! | GCGAAGAAACATAATTAAAA+TGG | - | chr4_4:67381910-67381929 | None:intergenic | 25.0% |
| !! | ATGACAATTTTTGTATCATC+TGG | + | chr4_4:67382021-67382040 | Msa0697380:intron | 25.0% |
| ! | ATAGCTTATTCTATGACAAT+TGG | - | chr4_4:67382123-67382142 | None:intergenic | 25.0% |
| !! | TTGATATTGGTGCTAAATTC+AGG | - | chr4_4:67381479-67381498 | None:intergenic | 30.0% |
| AAAGACAACAAAGGGAAATT+TGG | - | chr4_4:67381534-67381553 | None:intergenic | 30.0% | |
| GATGATTAATTCGATCAGTT+TGG | + | chr4_4:67381583-67381602 | Msa0697380:intron | 30.0% | |
| ! | ATCAGTTTGGTAATATCTGT+TGG | + | chr4_4:67381596-67381615 | Msa0697380:intron | 30.0% |
| ! | TCAGTTTGGTAATATCTGTT+GGG | + | chr4_4:67381597-67381616 | Msa0697380:intron | 30.0% |
| ACTTATCATGAACATAAAGC+AGG | - | chr4_4:67381651-67381670 | None:intergenic | 30.0% | |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | - | chr4_4:67381736-67381755 | None:intergenic | 30.0% |
| ! | TTTTGCAGTTAATATCCTTG+AGG | + | chr4_4:67381828-67381847 | Msa0697380:intron | 30.0% |
| TTTGCAGTTAATATCCTTGA+GGG | + | chr4_4:67381829-67381848 | Msa0697380:intron | 30.0% | |
| TTCTATGACAATTGGCATAA+GGG | - | chr4_4:67382115-67382134 | None:intergenic | 30.0% | |
| ATTCTATGACAATTGGCATA+AGG | - | chr4_4:67382116-67382135 | None:intergenic | 30.0% | |
| TTCTTTGTTAGTATCTGATG+TGG | + | chr4_4:67382266-67382285 | Msa0697380:intron | 30.0% | |
| ! | TATCTGATGTGGTTTAGTTT+GGG | + | chr4_4:67382277-67382296 | Msa0697380:three_prime_UTR | 30.0% |
| GTTAGACATGATGAAAACAA+AGG | + | chr4_4:67382337-67382356 | Msa0697380:three_prime_UTR | 30.0% | |
| ! | ATGAAAACAAAGGCATGTAA+GGG | + | chr4_4:67382347-67382366 | Msa0697380:three_prime_UTR | 30.0% |
| !! | TTGATATTGGTGCTAAATTC+AGG | - | chr4_4:67381479-67381498 | None:intergenic | 30.0% |
| AAAGACAACAAAGGGAAATT+TGG | - | chr4_4:67381534-67381553 | None:intergenic | 30.0% | |
| GATGATTAATTCGATCAGTT+TGG | + | chr4_4:67381583-67381602 | Msa0697380:intron | 30.0% | |
| ! | ATCAGTTTGGTAATATCTGT+TGG | + | chr4_4:67381596-67381615 | Msa0697380:intron | 30.0% |
| ! | TCAGTTTGGTAATATCTGTT+GGG | + | chr4_4:67381597-67381616 | Msa0697380:intron | 30.0% |
| ACTTATCATGAACATAAAGC+AGG | - | chr4_4:67381651-67381670 | None:intergenic | 30.0% | |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | - | chr4_4:67381736-67381755 | None:intergenic | 30.0% |
| ! | TTTTGCAGTTAATATCCTTG+AGG | + | chr4_4:67381828-67381847 | Msa0697380:intron | 30.0% |
| TTTGCAGTTAATATCCTTGA+GGG | + | chr4_4:67381829-67381848 | Msa0697380:intron | 30.0% | |
| TTCTATGACAATTGGCATAA+GGG | - | chr4_4:67382115-67382134 | None:intergenic | 30.0% | |
| ATTCTATGACAATTGGCATA+AGG | - | chr4_4:67382116-67382135 | None:intergenic | 30.0% | |
| TTCTTTGTTAGTATCTGATG+TGG | + | chr4_4:67382266-67382285 | Msa0697380:intron | 30.0% | |
| ! | TATCTGATGTGGTTTAGTTT+GGG | + | chr4_4:67382277-67382296 | Msa0697380:three_prime_UTR | 30.0% |
| GTTAGACATGATGAAAACAA+AGG | + | chr4_4:67382337-67382356 | Msa0697380:three_prime_UTR | 30.0% | |
| TTGAAAGTTGCAATGATGTC+AGG | - | chr4_4:67381124-67381143 | None:intergenic | 35.0% | |
| GCAACTTTCAAAGTCATTGA+TGG | + | chr4_4:67381133-67381152 | Msa0697380:CDS | 35.0% | |
| ! | CTATGAAGTTTTTCGTCGAA+TGG | + | chr4_4:67381175-67381194 | Msa0697380:CDS | 35.0% |
| TGAACTTCTTCACTATGGAA+TGG | + | chr4_4:67381248-67381267 | Msa0697380:intron | 35.0% | |
| AACTTTGCTCGCTAATATCT+TGG | + | chr4_4:67381319-67381338 | Msa0697380:intron | 35.0% | |
| ACTTTGCTCGCTAATATCTT+GGG | + | chr4_4:67381320-67381339 | Msa0697380:intron | 35.0% | |
| TGGTTGTTTAGCCTCTTAAA+AGG | + | chr4_4:67381722-67381741 | Msa0697380:intron | 35.0% | |
| GAGAGAAAAAAACGTACAGT+AGG | - | chr4_4:67381888-67381907 | None:intergenic | 35.0% | |
| CACATCTATTCGAAATGCTT+GGG | - | chr4_4:67381963-67381982 | None:intergenic | 35.0% | |
| TCACATCTATTCGAAATGCT+TGG | - | chr4_4:67381964-67381983 | None:intergenic | 35.0% | |
| AGCATTTCGAATAGATGTGA+TGG | + | chr4_4:67381964-67381983 | Msa0697380:intron | 35.0% | |
| GCATTTCGAATAGATGTGAT+GGG | + | chr4_4:67381965-67381984 | Msa0697380:intron | 35.0% | |
| ! | AATAGATGTGATGGGACAAA+AGG | + | chr4_4:67381973-67381992 | Msa0697380:intron | 35.0% |
| GTATCTGATGTGGTTTAGTT+TGG | + | chr4_4:67382276-67382295 | Msa0697380:intron | 35.0% | |
| !! | ATCTGATGTGGTTTAGTTTG+GGG | + | chr4_4:67382278-67382297 | Msa0697380:three_prime_UTR | 35.0% |
| ! | GATGAAAACAAAGGCATGTA+AGG | + | chr4_4:67382346-67382365 | Msa0697380:three_prime_UTR | 35.0% |
| TTGAAAGTTGCAATGATGTC+AGG | - | chr4_4:67381124-67381143 | None:intergenic | 35.0% | |
| GCAACTTTCAAAGTCATTGA+TGG | + | chr4_4:67381133-67381152 | Msa0697380:CDS | 35.0% | |
| ! | CTATGAAGTTTTTCGTCGAA+TGG | + | chr4_4:67381175-67381194 | Msa0697380:CDS | 35.0% |
| TGAACTTCTTCACTATGGAA+TGG | + | chr4_4:67381248-67381267 | Msa0697380:intron | 35.0% | |
| AACTTTGCTCGCTAATATCT+TGG | + | chr4_4:67381319-67381338 | Msa0697380:intron | 35.0% | |
| ACTTTGCTCGCTAATATCTT+GGG | + | chr4_4:67381320-67381339 | Msa0697380:intron | 35.0% | |
| TGGTTGTTTAGCCTCTTAAA+AGG | + | chr4_4:67381722-67381741 | Msa0697380:intron | 35.0% | |
| GAGAGAAAAAAACGTACAGT+AGG | - | chr4_4:67381888-67381907 | None:intergenic | 35.0% | |
| CACATCTATTCGAAATGCTT+GGG | - | chr4_4:67381963-67381982 | None:intergenic | 35.0% | |
| TCACATCTATTCGAAATGCT+TGG | - | chr4_4:67381964-67381983 | None:intergenic | 35.0% | |
| AGCATTTCGAATAGATGTGA+TGG | + | chr4_4:67381964-67381983 | Msa0697380:intron | 35.0% | |
| GCATTTCGAATAGATGTGAT+GGG | + | chr4_4:67381965-67381984 | Msa0697380:intron | 35.0% | |
| ! | AATAGATGTGATGGGACAAA+AGG | + | chr4_4:67381973-67381992 | Msa0697380:intron | 35.0% |
| GTATCTGATGTGGTTTAGTT+TGG | + | chr4_4:67382276-67382295 | Msa0697380:intron | 35.0% | |
| !! | ATCTGATGTGGTTTAGTTTG+GGG | + | chr4_4:67382278-67382297 | Msa0697380:three_prime_UTR | 35.0% |
| TCATAGACCGTTGATGTTTC+AGG | - | chr4_4:67381161-67381180 | None:intergenic | 40.0% | |
| AACGTACAGTAGGTGAATCT+AGG | - | chr4_4:67381878-67381897 | None:intergenic | 40.0% | |
| TCATAGACCGTTGATGTTTC+AGG | - | chr4_4:67381161-67381180 | None:intergenic | 40.0% | |
| AACGTACAGTAGGTGAATCT+AGG | - | chr4_4:67381878-67381897 | None:intergenic | 40.0% | |
| GATGGTGCCTGAAACATCAA+CGG | + | chr4_4:67381151-67381170 | Msa0697380:CDS | 45.0% | |
| CTAGAAGCAACAGTTCTGCA+AGG | + | chr4_4:67382306-67382325 | Msa0697380:three_prime_UTR | 45.0% | |
| GATGGTGCCTGAAACATCAA+CGG | + | chr4_4:67381151-67381170 | Msa0697380:CDS | 45.0% | |
| CTAGAAGCAACAGTTCTGCA+AGG | + | chr4_4:67382306-67382325 | Msa0697380:three_prime_UTR | 45.0% | |
| TGGTTGTCTCATCACCCTCA+AGG | - | chr4_4:67381846-67381865 | None:intergenic | 50.0% | |
| CAGTAGGTGAATCTAGGCGA+GGG | - | chr4_4:67381872-67381891 | None:intergenic | 50.0% | |
| ACAGTAGGTGAATCTAGGCG+AGG | - | chr4_4:67381873-67381892 | None:intergenic | 50.0% | |
| TGGTTGTCTCATCACCCTCA+AGG | - | chr4_4:67381846-67381865 | None:intergenic | 50.0% | |
| CAGTAGGTGAATCTAGGCGA+GGG | - | chr4_4:67381872-67381891 | None:intergenic | 50.0% | |
| ACAGTAGGTGAATCTAGGCG+AGG | - | chr4_4:67381873-67381892 | None:intergenic | 50.0% | |
| GTGAATCTAGGCGAGGGAAG+TGG | - | chr4_4:67381866-67381885 | None:intergenic | 55.0% | |
| GTGAATCTAGGCGAGGGAAG+TGG | - | chr4_4:67381866-67381885 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 67381110 | 67382366 | 67381110 | ID=Msa0697380;Name=Msa0697380 |
| chr4_4 | mRNA | 67381110 | 67382366 | 67381110 | ID=Msa0697380-mRNA-1;Parent=Msa0697380;Name=Msa0697380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=6|1|1|1|0.5|0.33|3|101|55 |
| chr4_4 | exon | 67381110 | 67381239 | 67381110 | ID=Msa0697380-mRNA-1:exon:19770;Parent=Msa0697380-mRNA-1 |
| chr4_4 | exon | 67381836 | 67381890 | 67381836 | ID=Msa0697380-mRNA-1:exon:19771;Parent=Msa0697380-mRNA-1 |
| chr4_4 | exon | 67382277 | 67382366 | 67382277 | ID=Msa0697380-mRNA-1:exon:19772;Parent=Msa0697380-mRNA-1 |
| chr4_4 | five_prime_UTR | 67381110 | 67381115 | 67381110 | ID=Msa0697380-mRNA-1:five_prime_utr;Parent=Msa0697380-mRNA-1 |
| chr4_4 | CDS | 67381116 | 67381239 | 67381116 | ID=Msa0697380-mRNA-1:cds;Parent=Msa0697380-mRNA-1 |
| chr4_4 | CDS | 67381836 | 67381879 | 67381836 | ID=Msa0697380-mRNA-1:cds;Parent=Msa0697380-mRNA-1 |
| chr4_4 | three_prime_UTR | 67381880 | 67381890 | 67381880 | ID=Msa0697380-mRNA-1:three_prime_utr;Parent=Msa0697380-mRNA-1 |
| chr4_4 | three_prime_UTR | 67382277 | 67382366 | 67382277 | ID=Msa0697380-mRNA-1:three_prime_utr;Parent=Msa0697380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
