AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0622460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0622460 MtrunA17_Chr7g0249251 95.312 128 5 1 4 130 45 172 5.26e-86 248
Msa0622460 MtrunA17_Chr7g0221761 50.000 88 37 3 30 115 64 146 1.05e-20 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0622460 AT5G16490.1 49.474 95 44 3 19 111 48 140 7.95e-20 80.1

Find 81 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCATACCTATACTTGTT+TGG 0.214133 4_2:+74892990 None:intergenic
ATGGGAGATGTTTCATGTTT+TGG 0.253272 4_2:+74891876 None:intergenic
TCTCATACCTATACTTGTTT+GGG 0.281024 4_2:+74892991 None:intergenic
GATGAAATGGACATGGAAAT+AGG 0.297095 4_2:-74892038 Msa0622460:CDS
TGAATTTAGTCATTCGATAA+AGG 0.317790 4_2:-74893303 Msa0622460:five_prime_UTR
GTCATTCTTCCTTTCTCAGC+TGG 0.335866 4_2:-74893591 Msa0622460:five_prime_UTR
TGGGGATAGGGTACTCTCTT+AGG 0.347139 4_2:+74893797 None:intergenic
ATATCATTAATGAATTAGTT+TGG 0.351066 4_2:+74893871 None:intergenic
GTGAATATCTCAATTGGAAT+TGG 0.354042 4_2:+74893653 None:intergenic
TTACTAGCTAAATAAAACTA+AGG 0.371765 4_2:+74893390 None:intergenic
TTGAAGGATAGGTTAGGCTT+TGG 0.378556 4_2:+74892696 None:intergenic
GATGATATGATAAGATTAGA+AGG 0.379519 4_2:-74892795 Msa0622460:exon
GGACTTAGAAGAAGATGAAA+TGG 0.383098 4_2:-74892051 Msa0622460:CDS
GTCTCATACTTTCTTATGAT+AGG 0.392213 4_2:+74893631 None:intergenic
AGAGAATCCCATCCCCTCAT+TGG 0.414199 4_2:+74891942 None:intergenic
TGATAGGTGAATATCTCAAT+TGG 0.415950 4_2:+74893647 None:intergenic
TCAATTAATCAAGCAATAGA+AGG 0.417530 4_2:+74891843 None:intergenic
GCCTTTGAACAACTTGTTGA+AGG 0.426359 4_2:+74892680 None:intergenic
GATATCGTAGTAAAAGAGAT+AGG 0.435495 4_2:+74893472 None:intergenic
AATATATGTTTAATAGAGAA+TGG 0.446012 4_2:+74893023 None:intergenic
ATGAAATGGACATGGAAATA+GGG 0.448347 4_2:-74892037 Msa0622460:CDS
GAGGATTCAGAGACTTTGTC+TGG 0.450700 4_2:-74892759 Msa0622460:CDS
AATAGAAGGCATGATGTTGA+TGG 0.460773 4_2:+74891857 None:intergenic
AACATGTGACACACATTGGT+TGG 0.460995 4_2:-74891998 Msa0622460:CDS
AGTTGTTATATCTCCATTCA+AGG 0.469327 4_2:-74893709 Msa0622460:five_prime_UTR
TGAACAACTTGTTGAAGGAT+AGG 0.476710 4_2:+74892685 None:intergenic
TGTGGTGAATTAGTCTCTGC+AGG 0.479443 4_2:+74893513 None:intergenic
TAGTCATTCGATAAAGGACT+TGG 0.489137 4_2:-74893297 Msa0622460:five_prime_UTR
AATTAGTTTGGTGAGTGAAA+GGG 0.489867 4_2:+74893883 None:intergenic
GAGAATCCCATCCCCTCATT+GGG 0.490983 4_2:+74891943 None:intergenic
ATAAGATTAGAAGGATCTAA+AGG 0.496998 4_2:-74892786 Msa0622460:CDS
ATACAATAAATTTGACCATC+GGG 0.499296 4_2:+74893066 None:intergenic
GAATTAGTTTGGTGAGTGAA+AGG 0.501822 4_2:+74893882 None:intergenic
AGTAAGACACACATGGGGAT+AGG 0.506123 4_2:+74893784 None:intergenic
TATGACAACATACCACCATA+TGG 0.511116 4_2:+74893219 None:intergenic
CTCATGACCCAATGAGGGGA+TGG 0.511835 4_2:-74891950 Msa0622460:CDS
GAGGCTACAGAGAGAAGCTC+AGG 0.512955 4_2:+74891912 None:intergenic
GTAAGACACACATGGGGATA+GGG 0.521490 4_2:+74893785 None:intergenic
AGAAAGTATGAGACAGAGAA+TGG 0.521813 4_2:-74893622 Msa0622460:five_prime_UTR
TCCTTCAACAAGTTGTTCAA+AGG 0.523718 4_2:-74892681 Msa0622460:CDS
GTCACATGTTGTACATCTGT+TGG 0.530235 4_2:+74892011 None:intergenic
TCACATGTTGTACATCTGTT+GGG 0.533947 4_2:+74892012 None:intergenic
GTTGTTATATCTCCATTCAA+GGG 0.534536 4_2:-74893708 Msa0622460:five_prime_UTR
GAGATACAACCAGCTGAGAA+AGG 0.537814 4_2:+74893582 None:intergenic
TCTGAGGCTAGTGTTGCTGT+TGG 0.544954 4_2:-74893561 Msa0622460:five_prime_UTR
TGTTTAATAGAGAATGGAAA+AGG 0.546481 4_2:+74893029 None:intergenic
AAAGTATGAAGAATTCTTTG+AGG 0.549357 4_2:-74892734 Msa0622460:CDS
ACATGTGACACACATTGGTT+GGG 0.559436 4_2:-74891997 Msa0622460:CDS
TCATGACCCAATGAGGGGAT+GGG 0.567190 4_2:-74891949 Msa0622460:CDS
TACTAGCTAAATAAAACTAA+GGG 0.567349 4_2:+74893391 None:intergenic
CTCATACCTATACTTGTTTG+GGG 0.569563 4_2:+74892992 None:intergenic
GATTCTCTCCCACGGTACAT+CGG 0.569850 4_2:-74893275 Msa0622460:five_prime_UTR
GAGAGAAGATCCGAGTCCGT+TGG 0.570167 4_2:-74893164 Msa0622460:five_prime_UTR
CATACAATAAATTTGACCAT+CGG 0.571124 4_2:+74893065 None:intergenic
TCAACTTCATCCAACGGACT+CGG 0.574514 4_2:+74893154 None:intergenic
TGCAAGAAAGTGTTGCCATA+TGG 0.578020 4_2:-74893234 Msa0622460:five_prime_UTR
CTCAGCTGGTTGTATCTCTG+AGG 0.578716 4_2:-74893577 Msa0622460:five_prime_UTR
ATAGAAGGCATGATGTTGAT+GGG 0.591363 4_2:+74891858 None:intergenic
AACTTGTTGAAGGATAGGTT+AGG 0.593453 4_2:+74892690 None:intergenic
GCTACAGAGAGAAGCTCAGG+AGG 0.604067 4_2:+74891915 None:intergenic
AATTGCAGTGGAGAAAGAAG+AGG 0.604469 4_2:-74892072 Msa0622460:intron
GAAAGTGTGATTCATTGTTG+TGG 0.607215 4_2:-74891772 Msa0622460:three_prime_UTR
TGGGTCATGAGCAGCACATG+AGG 0.609467 4_2:+74891962 None:intergenic
GTGACACACATTGGTTGGGA+TGG 0.614285 4_2:-74891993 Msa0622460:CDS
TGCTGCTCATGACCCAATGA+GGG 0.614602 4_2:-74891955 Msa0622460:CDS
TAATATAGTAAGACACACAT+GGG 0.623349 4_2:+74893778 None:intergenic
TATCAATCAACTTCATCCAA+CGG 0.629915 4_2:+74893148 None:intergenic
GTATGAGACAGAGAATGGAA+AGG 0.630032 4_2:-74893617 Msa0622460:five_prime_UTR
AGAAGAAGATGAAATGGACA+TGG 0.630572 4_2:-74892045 Msa0622460:CDS
TTTGTTGCACCGATGTACCG+TGG 0.630594 4_2:+74893266 None:intergenic
AGAAGGATCTAAAGGAGTCG+AGG 0.632435 4_2:-74892778 Msa0622460:CDS
TTGTTGCACCGATGTACCGT+GGG 0.632487 4_2:+74893267 None:intergenic
AAGAGACTTATTGCATCATG+TGG 0.639239 4_2:+74893495 None:intergenic
GTGCTGCTCATGACCCAATG+AGG 0.658635 4_2:-74891956 Msa0622460:CDS
TCATACCTATACTTGTTTGG+GGG 0.674696 4_2:+74892993 None:intergenic
AAGAAAGTGTTGCCATATGG+TGG 0.682164 4_2:-74893231 Msa0622460:five_prime_UTR
AGGACTTGGATTCTCTCCCA+CGG 0.683139 4_2:-74893283 Msa0622460:five_prime_UTR
ATAATATAGTAAGACACACA+TGG 0.703222 4_2:+74893777 None:intergenic
GCTGCTCATGACCCAATGAG+GGG 0.751010 4_2:-74891954 Msa0622460:CDS
GTACAACATGTGACACACAT+TGG 0.753481 4_2:-74892002 Msa0622460:CDS
AATATAGTAAGACACACATG+GGG 0.770159 4_2:+74893779 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTGTATTTTAAATTTTATT+TGG - chr4_2:74892535-74892554 Msa0622460:intron 10.0%
!!! ATTCTAAAAATGTTTATTTA+GGG + chr4_2:74893191-74893210 None:intergenic 10.0%
!! TCTTTGTAAATAAAAATATA+TGG - chr4_2:74893514-74893533 Msa0622460:five_prime_UTR 10.0%
!!! CTTGTATTTTAAATTTTATT+TGG - chr4_2:74892535-74892554 Msa0622460:intron 10.0%
!!! ATTCTAAAAATGTTTATTTA+GGG + chr4_2:74893191-74893210 None:intergenic 10.0%
!! TCTTTGTAAATAAAAATATA+TGG - chr4_2:74893514-74893533 Msa0622460:five_prime_UTR 10.0%
!! ATATCATTAATGAATTAGTT+TGG + chr4_2:74891785-74891804 None:intergenic 15.0%
!! AATATATGTTTAATAGAGAA+TGG + chr4_2:74892633-74892652 None:intergenic 15.0%
!!! TTTTTCTTTTTTGAAAGTTA+CGG + chr4_2:74892743-74892762 None:intergenic 15.0%
!!! TTCTCTAATTTTTAATTAGT+TGG - chr4_2:74892774-74892793 Msa0622460:CDS 15.0%
!!! CATTCTAAAAATGTTTATTT+AGG + chr4_2:74893192-74893211 None:intergenic 15.0%
!! CATAATAAAAATAAAACTGT+AGG - chr4_2:74893467-74893486 Msa0622460:five_prime_UTR 15.0%
!! ATATCATTAATGAATTAGTT+TGG + chr4_2:74891785-74891804 None:intergenic 15.0%
!! AATATATGTTTAATAGAGAA+TGG + chr4_2:74892633-74892652 None:intergenic 15.0%
!!! TTTTTCTTTTTTGAAAGTTA+CGG + chr4_2:74892743-74892762 None:intergenic 15.0%
!!! TTCTCTAATTTTTAATTAGT+TGG - chr4_2:74892774-74892793 Msa0622460:CDS 15.0%
!!! CATTCTAAAAATGTTTATTT+AGG + chr4_2:74893192-74893211 None:intergenic 15.0%
!! CATAATAAAAATAAAACTGT+AGG - chr4_2:74893467-74893486 Msa0622460:five_prime_UTR 15.0%
!! TACTAGCTAAATAAAACTAA+GGG + chr4_2:74892265-74892284 None:intergenic 20.0%
!! TTACTAGCTAAATAAAACTA+AGG + chr4_2:74892266-74892285 None:intergenic 20.0%
!! ATGTATAAAAAAAAATGTCG+TGG - chr4_2:74892687-74892706 Msa0622460:CDS 20.0%
!! TGTATAAAAAAAAATGTCGT+GGG - chr4_2:74892688-74892707 Msa0622460:CDS 20.0%
!!! ATAAAATTGGGTTTAAGATA+AGG + chr4_2:74893334-74893353 None:intergenic 20.0%
!! AAATTTATGTTTAATTGCAG+TGG - chr4_2:74893569-74893588 Msa0622460:five_prime_UTR 20.0%
!! TACTAGCTAAATAAAACTAA+GGG + chr4_2:74892265-74892284 None:intergenic 20.0%
!! TTACTAGCTAAATAAAACTA+AGG + chr4_2:74892266-74892285 None:intergenic 20.0%
!! ATGTATAAAAAAAAATGTCG+TGG - chr4_2:74892687-74892706 Msa0622460:CDS 20.0%
!! TGTATAAAAAAAAATGTCGT+GGG - chr4_2:74892688-74892707 Msa0622460:CDS 20.0%
!!! ATAAAATTGGGTTTAAGATA+AGG + chr4_2:74893334-74893353 None:intergenic 20.0%
!! AAATTTATGTTTAATTGCAG+TGG - chr4_2:74893569-74893588 Msa0622460:five_prime_UTR 20.0%
! TAATATAGTAAGACACACAT+GGG + chr4_2:74891878-74891897 None:intergenic 25.0%
! ATAATATAGTAAGACACACA+TGG + chr4_2:74891879-74891898 None:intergenic 25.0%
! TGAATTTAGTCATTCGATAA+AGG - chr4_2:74892350-74892369 Msa0622460:intron 25.0%
! ATACAATAAATTTGACCATC+GGG + chr4_2:74892590-74892609 None:intergenic 25.0%
! CATACAATAAATTTGACCAT+CGG + chr4_2:74892591-74892610 None:intergenic 25.0%
! TGTTTAATAGAGAATGGAAA+AGG + chr4_2:74892627-74892646 None:intergenic 25.0%
! GTATAAAAAAAAATGTCGTG+GGG - chr4_2:74892689-74892708 Msa0622460:CDS 25.0%
! GATGATATGATAAGATTAGA+AGG - chr4_2:74892858-74892877 Msa0622460:five_prime_UTR 25.0%
! ATAAGATTAGAAGGATCTAA+AGG - chr4_2:74892867-74892886 Msa0622460:five_prime_UTR 25.0%
! AAAGTATGAAGAATTCTTTG+AGG - chr4_2:74892919-74892938 Msa0622460:five_prime_UTR 25.0%
!! AAGAACTTTTCTCAATTGTT+TGG - chr4_2:74892999-74893018 Msa0622460:five_prime_UTR 25.0%
!! GTTTTCTCATACTAATTTTG+TGG - chr4_2:74893040-74893059 Msa0622460:five_prime_UTR 25.0%
! GAACGAAATAAAAACAAACA+AGG + chr4_2:74893129-74893148 None:intergenic 25.0%
!! TTTAAGATAAGGAAAGTTAG+AGG + chr4_2:74893323-74893342 None:intergenic 25.0%
!! TAAACCCAATTTTATAGAGT+CGG - chr4_2:74893339-74893358 Msa0622460:five_prime_UTR 25.0%
!! ATGGAAGTCTTAATTTTTCA+TGG - chr4_2:74893533-74893552 Msa0622460:five_prime_UTR 25.0%
! TCAATTAATCAAGCAATAGA+AGG + chr4_2:74893813-74893832 None:intergenic 25.0%
! TAATATAGTAAGACACACAT+GGG + chr4_2:74891878-74891897 None:intergenic 25.0%
! ATAATATAGTAAGACACACA+TGG + chr4_2:74891879-74891898 None:intergenic 25.0%
! TGAATTTAGTCATTCGATAA+AGG - chr4_2:74892350-74892369 Msa0622460:intron 25.0%
! ATACAATAAATTTGACCATC+GGG + chr4_2:74892590-74892609 None:intergenic 25.0%
! CATACAATAAATTTGACCAT+CGG + chr4_2:74892591-74892610 None:intergenic 25.0%
! TGTTTAATAGAGAATGGAAA+AGG + chr4_2:74892627-74892646 None:intergenic 25.0%
! GTATAAAAAAAAATGTCGTG+GGG - chr4_2:74892689-74892708 Msa0622460:CDS 25.0%
! GATGATATGATAAGATTAGA+AGG - chr4_2:74892858-74892877 Msa0622460:five_prime_UTR 25.0%
! ATAAGATTAGAAGGATCTAA+AGG - chr4_2:74892867-74892886 Msa0622460:five_prime_UTR 25.0%
! AAAGTATGAAGAATTCTTTG+AGG - chr4_2:74892919-74892938 Msa0622460:five_prime_UTR 25.0%
!! AAGAACTTTTCTCAATTGTT+TGG - chr4_2:74892999-74893018 Msa0622460:five_prime_UTR 25.0%
!! GTTTTCTCATACTAATTTTG+TGG - chr4_2:74893040-74893059 Msa0622460:five_prime_UTR 25.0%
! GAACGAAATAAAAACAAACA+AGG + chr4_2:74893129-74893148 None:intergenic 25.0%
!! TTTAAGATAAGGAAAGTTAG+AGG + chr4_2:74893323-74893342 None:intergenic 25.0%
!! TAAACCCAATTTTATAGAGT+CGG - chr4_2:74893339-74893358 Msa0622460:five_prime_UTR 25.0%
!! ATGGAAGTCTTAATTTTTCA+TGG - chr4_2:74893533-74893552 Msa0622460:five_prime_UTR 25.0%
! TCAATTAATCAAGCAATAGA+AGG + chr4_2:74893813-74893832 None:intergenic 25.0%
! AATTAGTTTGGTGAGTGAAA+GGG + chr4_2:74891773-74891792 None:intergenic 30.0%
AATATAGTAAGACACACATG+GGG + chr4_2:74891877-74891896 None:intergenic 30.0%
AGTTGTTATATCTCCATTCA+AGG - chr4_2:74891944-74891963 Msa0622460:CDS 30.0%
GTTGTTATATCTCCATTCAA+GGG - chr4_2:74891945-74891964 Msa0622460:CDS 30.0%
GTGAATATCTCAATTGGAAT+TGG + chr4_2:74892003-74892022 None:intergenic 30.0%
TGATAGGTGAATATCTCAAT+TGG + chr4_2:74892009-74892028 None:intergenic 30.0%
GTCTCATACTTTCTTATGAT+AGG + chr4_2:74892025-74892044 None:intergenic 30.0%
GATATCGTAGTAAAAGAGAT+AGG + chr4_2:74892184-74892203 None:intergenic 30.0%
TATCAATCAACTTCATCCAA+CGG + chr4_2:74892508-74892527 None:intergenic 30.0%
TCTCATACCTATACTTGTTT+GGG + chr4_2:74892665-74892684 None:intergenic 30.0%
TTCTCATACCTATACTTGTT+TGG + chr4_2:74892666-74892685 None:intergenic 30.0%
CTCGATCATCTCAATATATA+AGG - chr4_2:74893223-74893242 Msa0622460:five_prime_UTR 30.0%
TCATCTCAATATATAAGGTG+TGG - chr4_2:74893228-74893247 Msa0622460:five_prime_UTR 30.0%
GAAGAAATACGTGTCAATAA+TGG - chr4_2:74893263-74893282 Msa0622460:five_prime_UTR 30.0%
ATGAAATGGACATGGAAATA+GGG - chr4_2:74893616-74893635 Msa0622460:five_prime_UTR 30.0%
! AATTAGTTTGGTGAGTGAAA+GGG + chr4_2:74891773-74891792 None:intergenic 30.0%
AATATAGTAAGACACACATG+GGG + chr4_2:74891877-74891896 None:intergenic 30.0%
AGTTGTTATATCTCCATTCA+AGG - chr4_2:74891944-74891963 Msa0622460:CDS 30.0%
GTTGTTATATCTCCATTCAA+GGG - chr4_2:74891945-74891964 Msa0622460:CDS 30.0%
GTGAATATCTCAATTGGAAT+TGG + chr4_2:74892003-74892022 None:intergenic 30.0%
TGATAGGTGAATATCTCAAT+TGG + chr4_2:74892009-74892028 None:intergenic 30.0%
GTCTCATACTTTCTTATGAT+AGG + chr4_2:74892025-74892044 None:intergenic 30.0%
GATATCGTAGTAAAAGAGAT+AGG + chr4_2:74892184-74892203 None:intergenic 30.0%
TATCAATCAACTTCATCCAA+CGG + chr4_2:74892508-74892527 None:intergenic 30.0%
TCTCATACCTATACTTGTTT+GGG + chr4_2:74892665-74892684 None:intergenic 30.0%
TTCTCATACCTATACTTGTT+TGG + chr4_2:74892666-74892685 None:intergenic 30.0%
CTCGATCATCTCAATATATA+AGG - chr4_2:74893223-74893242 Msa0622460:five_prime_UTR 30.0%
TCATCTCAATATATAAGGTG+TGG - chr4_2:74893228-74893247 Msa0622460:five_prime_UTR 30.0%
GAAGAAATACGTGTCAATAA+TGG - chr4_2:74893263-74893282 Msa0622460:five_prime_UTR 30.0%
ATGAAATGGACATGGAAATA+GGG - chr4_2:74893616-74893635 Msa0622460:five_prime_UTR 30.0%
! GAATTAGTTTGGTGAGTGAA+AGG + chr4_2:74891774-74891793 None:intergenic 35.0%
CAAAAAATGCAACCCTTGAA+TGG + chr4_2:74891960-74891979 None:intergenic 35.0%
!!! CATTCAAGGGTTGCATTTTT+TGG - chr4_2:74891958-74891977 Msa0622460:CDS 35.0%
! AGAAAGTATGAGACAGAGAA+TGG - chr4_2:74892031-74892050 Msa0622460:CDS 35.0%
AAGAGACTTATTGCATCATG+TGG + chr4_2:74892161-74892180 None:intergenic 35.0%
TAGTCATTCGATAAAGGACT+TGG - chr4_2:74892356-74892375 Msa0622460:intron 35.0%
TATGACAACATACCACCATA+TGG + chr4_2:74892437-74892456 None:intergenic 35.0%
TCATACCTATACTTGTTTGG+GGG + chr4_2:74892663-74892682 None:intergenic 35.0%
CTCATACCTATACTTGTTTG+GGG + chr4_2:74892664-74892683 None:intergenic 35.0%
!! AACTTGTTGAAGGATAGGTT+AGG + chr4_2:74892966-74892985 None:intergenic 35.0%
!! TGAACAACTTGTTGAAGGAT+AGG + chr4_2:74892971-74892990 None:intergenic 35.0%
TCCTTCAACAAGTTGTTCAA+AGG - chr4_2:74892972-74892991 Msa0622460:five_prime_UTR 35.0%
TTGAGATGATCGAGACTAAA+AGG + chr4_2:74893217-74893236 None:intergenic 35.0%
ATAAGGAAAGTTAGAGGTAG+GGG + chr4_2:74893317-74893336 None:intergenic 35.0%
GATAAGGAAAGTTAGAGGTA+GGG + chr4_2:74893318-74893337 None:intergenic 35.0%
AGATAAGGAAAGTTAGAGGT+AGG + chr4_2:74893319-74893338 None:intergenic 35.0%
CACTCCGACTCTATAAAATT+GGG + chr4_2:74893346-74893365 None:intergenic 35.0%
GGACTTAGAAGAAGATGAAA+TGG - chr4_2:74893602-74893621 Msa0622460:five_prime_UTR 35.0%
AGAAGAAGATGAAATGGACA+TGG - chr4_2:74893608-74893627 Msa0622460:five_prime_UTR 35.0%
GATGAAATGGACATGGAAAT+AGG - chr4_2:74893615-74893634 Msa0622460:five_prime_UTR 35.0%
TCACATGTTGTACATCTGTT+GGG + chr4_2:74893644-74893663 None:intergenic 35.0%
!! TTTTGGCTGTAAAGATTGAG+AGG + chr4_2:74893763-74893782 None:intergenic 35.0%
! ATGGGAGATGTTTCATGTTT+TGG + chr4_2:74893780-74893799 None:intergenic 35.0%
! ATAGAAGGCATGATGTTGAT+GGG + chr4_2:74893798-74893817 None:intergenic 35.0%
! AATAGAAGGCATGATGTTGA+TGG + chr4_2:74893799-74893818 None:intergenic 35.0%
! GAAAGTGTGATTCATTGTTG+TGG - chr4_2:74893881-74893900 Msa0622460:five_prime_UTR 35.0%
! GAATTAGTTTGGTGAGTGAA+AGG + chr4_2:74891774-74891793 None:intergenic 35.0%
CAAAAAATGCAACCCTTGAA+TGG + chr4_2:74891960-74891979 None:intergenic 35.0%
!!! CATTCAAGGGTTGCATTTTT+TGG - chr4_2:74891958-74891977 Msa0622460:CDS 35.0%
! AGAAAGTATGAGACAGAGAA+TGG - chr4_2:74892031-74892050 Msa0622460:CDS 35.0%
AAGAGACTTATTGCATCATG+TGG + chr4_2:74892161-74892180 None:intergenic 35.0%
TAGTCATTCGATAAAGGACT+TGG - chr4_2:74892356-74892375 Msa0622460:intron 35.0%
TATGACAACATACCACCATA+TGG + chr4_2:74892437-74892456 None:intergenic 35.0%
TCATACCTATACTTGTTTGG+GGG + chr4_2:74892663-74892682 None:intergenic 35.0%
CTCATACCTATACTTGTTTG+GGG + chr4_2:74892664-74892683 None:intergenic 35.0%
!! AACTTGTTGAAGGATAGGTT+AGG + chr4_2:74892966-74892985 None:intergenic 35.0%
!! TGAACAACTTGTTGAAGGAT+AGG + chr4_2:74892971-74892990 None:intergenic 35.0%
TCCTTCAACAAGTTGTTCAA+AGG - chr4_2:74892972-74892991 Msa0622460:five_prime_UTR 35.0%
TTGAGATGATCGAGACTAAA+AGG + chr4_2:74893217-74893236 None:intergenic 35.0%
ATAAGGAAAGTTAGAGGTAG+GGG + chr4_2:74893317-74893336 None:intergenic 35.0%
GATAAGGAAAGTTAGAGGTA+GGG + chr4_2:74893318-74893337 None:intergenic 35.0%
AGATAAGGAAAGTTAGAGGT+AGG + chr4_2:74893319-74893338 None:intergenic 35.0%
CACTCCGACTCTATAAAATT+GGG + chr4_2:74893346-74893365 None:intergenic 35.0%
GGACTTAGAAGAAGATGAAA+TGG - chr4_2:74893602-74893621 Msa0622460:five_prime_UTR 35.0%
AGAAGAAGATGAAATGGACA+TGG - chr4_2:74893608-74893627 Msa0622460:five_prime_UTR 35.0%
GATGAAATGGACATGGAAAT+AGG - chr4_2:74893615-74893634 Msa0622460:five_prime_UTR 35.0%
TCACATGTTGTACATCTGTT+GGG + chr4_2:74893644-74893663 None:intergenic 35.0%
!! TTTTGGCTGTAAAGATTGAG+AGG + chr4_2:74893763-74893782 None:intergenic 35.0%
! ATGGGAGATGTTTCATGTTT+TGG + chr4_2:74893780-74893799 None:intergenic 35.0%
! ATAGAAGGCATGATGTTGAT+GGG + chr4_2:74893798-74893817 None:intergenic 35.0%
! AATAGAAGGCATGATGTTGA+TGG + chr4_2:74893799-74893818 None:intergenic 35.0%
! GAAAGTGTGATTCATTGTTG+TGG - chr4_2:74893881-74893900 Msa0622460:five_prime_UTR 35.0%
!!! TCAAGGGTTGCATTTTTTGG+TGG - chr4_2:74891961-74891980 Msa0622460:CDS 40.0%
GTATGAGACAGAGAATGGAA+AGG - chr4_2:74892036-74892055 Msa0622460:CDS 40.0%
!! CTGCAGGTTTTGATCTTCTT+GGG + chr4_2:74892127-74892146 None:intergenic 40.0%
!! TCTGCAGGTTTTGATCTTCT+TGG + chr4_2:74892128-74892147 None:intergenic 40.0%
!! TGCAAGAAAGTGTTGCCATA+TGG - chr4_2:74892419-74892438 Msa0622460:intron 40.0%
!! AAGAAAGTGTTGCCATATGG+TGG - chr4_2:74892422-74892441 Msa0622460:intron 40.0%
!!! AACGATGTATTTTGACCCGA+TGG - chr4_2:74892572-74892591 Msa0622460:intron 40.0%
TTCTTCCCCCAAACAAGTAT+AGG - chr4_2:74892655-74892674 Msa0622460:CDS 40.0%
TTGAAGGATAGGTTAGGCTT+TGG + chr4_2:74892960-74892979 None:intergenic 40.0%
!! GCCTTTGAACAACTTGTTGA+AGG + chr4_2:74892976-74892995 None:intergenic 40.0%
AAAGTTAGAGGTAGGGGAAA+TGG + chr4_2:74893311-74893330 None:intergenic 40.0%
CCACTCCGACTCTATAAAAT+TGG + chr4_2:74893347-74893366 None:intergenic 40.0%
! CCAATTTTATAGAGTCGGAG+TGG - chr4_2:74893344-74893363 Msa0622460:five_prime_UTR 40.0%
AATTGCAGTGGAGAAAGAAG+AGG - chr4_2:74893581-74893600 Msa0622460:five_prime_UTR 40.0%
GTCACATGTTGTACATCTGT+TGG + chr4_2:74893645-74893664 None:intergenic 40.0%
GTACAACATGTGACACACAT+TGG - chr4_2:74893651-74893670 Msa0622460:five_prime_UTR 40.0%
AACATGTGACACACATTGGT+TGG - chr4_2:74893655-74893674 Msa0622460:five_prime_UTR 40.0%
! ACATGTGACACACATTGGTT+GGG - chr4_2:74893656-74893675 Msa0622460:five_prime_UTR 40.0%
!!! TCAAGGGTTGCATTTTTTGG+TGG - chr4_2:74891961-74891980 Msa0622460:CDS 40.0%
GTATGAGACAGAGAATGGAA+AGG - chr4_2:74892036-74892055 Msa0622460:CDS 40.0%
!! CTGCAGGTTTTGATCTTCTT+GGG + chr4_2:74892127-74892146 None:intergenic 40.0%
!! TCTGCAGGTTTTGATCTTCT+TGG + chr4_2:74892128-74892147 None:intergenic 40.0%
!! TGCAAGAAAGTGTTGCCATA+TGG - chr4_2:74892419-74892438 Msa0622460:intron 40.0%
!! AAGAAAGTGTTGCCATATGG+TGG - chr4_2:74892422-74892441 Msa0622460:intron 40.0%
!!! AACGATGTATTTTGACCCGA+TGG - chr4_2:74892572-74892591 Msa0622460:intron 40.0%
TTCTTCCCCCAAACAAGTAT+AGG - chr4_2:74892655-74892674 Msa0622460:CDS 40.0%
TTGAAGGATAGGTTAGGCTT+TGG + chr4_2:74892960-74892979 None:intergenic 40.0%
!! GCCTTTGAACAACTTGTTGA+AGG + chr4_2:74892976-74892995 None:intergenic 40.0%
AAAGTTAGAGGTAGGGGAAA+TGG + chr4_2:74893311-74893330 None:intergenic 40.0%
CCACTCCGACTCTATAAAAT+TGG + chr4_2:74893347-74893366 None:intergenic 40.0%
! CCAATTTTATAGAGTCGGAG+TGG - chr4_2:74893344-74893363 Msa0622460:five_prime_UTR 40.0%
AATTGCAGTGGAGAAAGAAG+AGG - chr4_2:74893581-74893600 Msa0622460:five_prime_UTR 40.0%
GTCACATGTTGTACATCTGT+TGG + chr4_2:74893645-74893664 None:intergenic 40.0%
GTACAACATGTGACACACAT+TGG - chr4_2:74893651-74893670 Msa0622460:five_prime_UTR 40.0%
AACATGTGACACACATTGGT+TGG - chr4_2:74893655-74893674 Msa0622460:five_prime_UTR 40.0%
! ACATGTGACACACATTGGTT+GGG - chr4_2:74893656-74893675 Msa0622460:five_prime_UTR 40.0%
GTAAGACACACATGGGGATA+GGG + chr4_2:74891871-74891890 None:intergenic 45.0%
AGTAAGACACACATGGGGAT+AGG + chr4_2:74891872-74891891 None:intergenic 45.0%
GTCATTCTTCCTTTCTCAGC+TGG - chr4_2:74892062-74892081 Msa0622460:CDS 45.0%
GAGATACAACCAGCTGAGAA+AGG + chr4_2:74892074-74892093 None:intergenic 45.0%
!! GGTTTTGATCTTCTTGGGTG+TGG + chr4_2:74892122-74892141 None:intergenic 45.0%
! TGTGGTGAATTAGTCTCTGC+AGG + chr4_2:74892143-74892162 None:intergenic 45.0%
! TCAACTTCATCCAACGGACT+CGG + chr4_2:74892502-74892521 None:intergenic 45.0%
AGAAGGATCTAAAGGAGTCG+AGG - chr4_2:74892875-74892894 Msa0622460:five_prime_UTR 45.0%
GAGGATTCAGAGACTTTGTC+TGG - chr4_2:74892894-74892913 Msa0622460:five_prime_UTR 45.0%
GTAAGACACACATGGGGATA+GGG + chr4_2:74891871-74891890 None:intergenic 45.0%
AGTAAGACACACATGGGGAT+AGG + chr4_2:74891872-74891891 None:intergenic 45.0%
GTCATTCTTCCTTTCTCAGC+TGG - chr4_2:74892062-74892081 Msa0622460:CDS 45.0%
GAGATACAACCAGCTGAGAA+AGG + chr4_2:74892074-74892093 None:intergenic 45.0%
!! GGTTTTGATCTTCTTGGGTG+TGG + chr4_2:74892122-74892141 None:intergenic 45.0%
! TGTGGTGAATTAGTCTCTGC+AGG + chr4_2:74892143-74892162 None:intergenic 45.0%
! TCAACTTCATCCAACGGACT+CGG + chr4_2:74892502-74892521 None:intergenic 45.0%
AGAAGGATCTAAAGGAGTCG+AGG - chr4_2:74892875-74892894 Msa0622460:five_prime_UTR 45.0%
GAGGATTCAGAGACTTTGTC+TGG - chr4_2:74892894-74892913 Msa0622460:five_prime_UTR 45.0%
!! AAAAGATATAAATTAATATA+TGG + chr4_2:74893067-74893086 None:intergenic 5.0%
!! AAAAGATATAAATTAATATA+TGG + chr4_2:74893067-74893086 None:intergenic 5.0%
! TGGGGATAGGGTACTCTCTT+AGG + chr4_2:74891859-74891878 None:intergenic 50.0%
CTCAGCTGGTTGTATCTCTG+AGG - chr4_2:74892076-74892095 Msa0622460:intron 50.0%
! TCTGAGGCTAGTGTTGCTGT+TGG - chr4_2:74892092-74892111 Msa0622460:intron 50.0%
AGGACTTGGATTCTCTCCCA+CGG - chr4_2:74892370-74892389 Msa0622460:intron 50.0%
GATTCTCTCCCACGGTACAT+CGG - chr4_2:74892378-74892397 Msa0622460:intron 50.0%
! TTGTTGCACCGATGTACCGT+GGG + chr4_2:74892389-74892408 None:intergenic 50.0%
! TTTGTTGCACCGATGTACCG+TGG + chr4_2:74892390-74892409 None:intergenic 50.0%
TTGGTGAGTATGCAAGCGTG+TGG - chr4_2:74893018-74893037 Msa0622460:five_prime_UTR 50.0%
AGTCGGAGTGGTGAGAAGAT+GGG - chr4_2:74893356-74893375 Msa0622460:five_prime_UTR 50.0%
! GTGACACACATTGGTTGGGA+TGG - chr4_2:74893660-74893679 Msa0622460:five_prime_UTR 50.0%
TGCTGCTCATGACCCAATGA+GGG - chr4_2:74893698-74893717 Msa0622460:five_prime_UTR 50.0%
TCATGACCCAATGAGGGGAT+GGG - chr4_2:74893704-74893723 Msa0622460:five_prime_UTR 50.0%
GAGAATCCCATCCCCTCATT+GGG + chr4_2:74893713-74893732 None:intergenic 50.0%
AGAGAATCCCATCCCCTCAT+TGG + chr4_2:74893714-74893733 None:intergenic 50.0%
! TGGGGATAGGGTACTCTCTT+AGG + chr4_2:74891859-74891878 None:intergenic 50.0%
CTCAGCTGGTTGTATCTCTG+AGG - chr4_2:74892076-74892095 Msa0622460:intron 50.0%
! TCTGAGGCTAGTGTTGCTGT+TGG - chr4_2:74892092-74892111 Msa0622460:intron 50.0%
AGGACTTGGATTCTCTCCCA+CGG - chr4_2:74892370-74892389 Msa0622460:intron 50.0%
GATTCTCTCCCACGGTACAT+CGG - chr4_2:74892378-74892397 Msa0622460:intron 50.0%
! TTGTTGCACCGATGTACCGT+GGG + chr4_2:74892389-74892408 None:intergenic 50.0%
! TTTGTTGCACCGATGTACCG+TGG + chr4_2:74892390-74892409 None:intergenic 50.0%
TTGGTGAGTATGCAAGCGTG+TGG - chr4_2:74893018-74893037 Msa0622460:five_prime_UTR 50.0%
AGTCGGAGTGGTGAGAAGAT+GGG - chr4_2:74893356-74893375 Msa0622460:five_prime_UTR 50.0%
! GTGACACACATTGGTTGGGA+TGG - chr4_2:74893660-74893679 Msa0622460:five_prime_UTR 50.0%
TGCTGCTCATGACCCAATGA+GGG - chr4_2:74893698-74893717 Msa0622460:five_prime_UTR 50.0%
TCATGACCCAATGAGGGGAT+GGG - chr4_2:74893704-74893723 Msa0622460:five_prime_UTR 50.0%
GAGAATCCCATCCCCTCATT+GGG + chr4_2:74893713-74893732 None:intergenic 50.0%
AGAGAATCCCATCCCCTCAT+TGG + chr4_2:74893714-74893733 None:intergenic 50.0%
GAGAGAAGATCCGAGTCCGT+TGG - chr4_2:74892489-74892508 Msa0622460:intron 55.0%
GAGTCGGAGTGGTGAGAAGA+TGG - chr4_2:74893355-74893374 Msa0622460:five_prime_UTR 55.0%
TGGGTCATGAGCAGCACATG+AGG + chr4_2:74893694-74893713 None:intergenic 55.0%
! GTGCTGCTCATGACCCAATG+AGG - chr4_2:74893697-74893716 Msa0622460:five_prime_UTR 55.0%
GCTGCTCATGACCCAATGAG+GGG - chr4_2:74893699-74893718 Msa0622460:five_prime_UTR 55.0%
CTCATGACCCAATGAGGGGA+TGG - chr4_2:74893703-74893722 Msa0622460:five_prime_UTR 55.0%
GCTACAGAGAGAAGCTCAGG+AGG + chr4_2:74893741-74893760 None:intergenic 55.0%
GAGGCTACAGAGAGAAGCTC+AGG + chr4_2:74893744-74893763 None:intergenic 55.0%
GAGAGAAGATCCGAGTCCGT+TGG - chr4_2:74892489-74892508 Msa0622460:intron 55.0%
GAGTCGGAGTGGTGAGAAGA+TGG - chr4_2:74893355-74893374 Msa0622460:five_prime_UTR 55.0%
TGGGTCATGAGCAGCACATG+AGG + chr4_2:74893694-74893713 None:intergenic 55.0%
! GTGCTGCTCATGACCCAATG+AGG - chr4_2:74893697-74893716 Msa0622460:five_prime_UTR 55.0%
GCTGCTCATGACCCAATGAG+GGG - chr4_2:74893699-74893718 Msa0622460:five_prime_UTR 55.0%
CTCATGACCCAATGAGGGGA+TGG - chr4_2:74893703-74893722 Msa0622460:five_prime_UTR 55.0%
GCTACAGAGAGAAGCTCAGG+AGG + chr4_2:74893741-74893760 None:intergenic 55.0%
GAGGCTACAGAGAGAAGCTC+AGG + chr4_2:74893744-74893763 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 74891763 74893912 74891763 ID=Msa0622460;Name=Msa0622460
chr4_2 mRNA 74891763 74893912 74891763 ID=Msa0622460-mRNA-1;Parent=Msa0622460;Name=Msa0622460-mRNA-1;_AED=0.01;_eAED=0.01;_QI=1101|1|1|1|1|1|2|88|130
chr4_2 exon 74891763 74892086 74891763 ID=Msa0622460-mRNA-1:exon:22711;Parent=Msa0622460-mRNA-1
chr4_2 exon 74892655 74893912 74892655 ID=Msa0622460-mRNA-1:exon:22710;Parent=Msa0622460-mRNA-1
chr4_2 five_prime_UTR 74892812 74893912 74892812 ID=Msa0622460-mRNA-1:five_prime_utr;Parent=Msa0622460-mRNA-1
chr4_2 CDS 74892655 74892811 74892655 ID=Msa0622460-mRNA-1:cds;Parent=Msa0622460-mRNA-1
chr4_2 CDS 74891851 74892086 74891851 ID=Msa0622460-mRNA-1:cds;Parent=Msa0622460-mRNA-1
chr4_2 three_prime_UTR 74891763 74891850 74891763 ID=Msa0622460-mRNA-1:three_prime_utr;Parent=Msa0622460-mRNA-1
Gene Sequence

>Msa0622460

ATGATAAGATTAGAAGGATCTAAAGGAGTCGAGGATTCAGAGACTTTGTCTGGTGAAAGTATGAAGAATTCTTTGAGGTTACTTGATGTTGTTCCAAAGCCTAACCTATCCTTCAACAAGTTGTTCAAAGGCTTCAAGAACTTTTCTCAATTGTTTGTGGAGAAAGAAGAGGACTTAGAAGAAGATGAAATGGACATGGAAATAGGGTGCCCAACAGATGTACAACATGTGACACACATTGGTTGGGATGGTGTTACAACCTCATGTGCTGCTCATGACCCAATGAGGGGATGGGATTCTCTCATTCCTCCTGAGCTTCTCTCTGTAGCCTCTCAATCTTTACAGCCAAAACATGAAACATCTCCCATCAACATCATGCCTTCTATTGCTTGA
Protein sequence

>Msa0622460

MIRLEGSKGVEDSETLSGESMKNSLRLLDVVPKPNLSFNKLFKGFKNFSQLFVEKEEDLEEDEMDMEIGCPTDVQHVTHIGWDGVTTSCAAHDPMRGWDSLIPPELLSVASQSLQPKHETSPINIMPSIA*