AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0623960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623960 MtrunA17_Chr8g0375301 67.213 61 15 1 4 59 30 90 9.55e-21 83.6
Msa0623960 MtrunA17_Chr5g0433921 52.459 61 24 1 4 59 30 90 4.58e-15 63.9
Msa0623960 MtrunA17_Chr6g0480631 45.714 70 33 1 4 68 33 102 8.16e-15 67.0
Msa0623960 MtrunA17_Chr3g0109901 45.714 70 33 1 4 68 30 99 5.12e-14 64.7
Msa0623960 MtrunA17_Chr4g0038791 73.684 38 10 0 6 43 1 38 2.77e-13 57.8
Msa0623960 MtrunA17_Chr4g0038801 71.429 42 10 1 4 45 19 58 5.81e-13 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623960 AT2G02010.1 50.000 70 30 1 4 68 30 99 9.22e-17 72.8
Msa0623960 AT2G02010.2 50.000 70 30 1 4 68 82 151 7.08e-16 70.5
Msa0623960 AT5G17330.1 48.571 70 31 1 4 68 30 99 3.49e-15 68.6
Msa0623960 AT3G17760.2 44.286 70 34 1 4 68 29 98 4.08e-14 65.5
Msa0623960 AT3G17760.1 44.286 70 34 1 4 68 29 98 4.08e-14 65.5
Msa0623960 AT2G02000.1 42.857 70 35 1 4 68 30 99 1.31e-12 61.2
Msa0623960 AT1G65960.2 42.857 70 35 1 4 68 29 98 1.93e-12 60.5

Find 33 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGTTTGTCCAAGAATT+TGG 0.102194 4_3:-1782218 None:intergenic
AGAACCAAACGAAGCAAATA+AGG 0.330837 4_3:+1781972 Msa0623960:CDS
TATTCCTTATTTGCTTCGTT+TGG 0.353520 4_3:-1781976 None:intergenic
ATGGAAATCATGTTCAACTT+TGG 0.357144 4_3:-1781867 None:intergenic
TGGAAATCATGTTCAACTTT+GGG 0.363923 4_3:-1781866 None:intergenic
TTATGGTATACAACTTCCTT+TGG 0.381162 4_3:-1781813 None:intergenic
ATTTGTGTCACAATAATCAT+TGG 0.382968 4_3:-1782408 None:intergenic
CATACATGATAAGCTGTTGT+TGG 0.391560 4_3:+1781836 Msa0623960:CDS
ACAGCTTATCATGTATGTTA+TGG 0.438601 4_3:-1781830 None:intergenic
AGGAATAGCGTTGGTGGAAA+AGG 0.441467 4_3:+1781992 Msa0623960:exon
ACATATACTGCAATGTGGTA+TGG 0.442915 4_3:-1782192 None:intergenic
TTCTAGCATATGTAGGTATC+CGG 0.451200 4_3:-1782154 None:intergenic
CATATACTGCAATGTGGTAT+GGG 0.464419 4_3:-1782191 None:intergenic
CTACCAGCACAAGGAGTATC+CGG 0.486689 4_3:+1782321 Msa0623960:three_prime_UTR
CAAATAAGGAATAGCGTTGG+TGG 0.493624 4_3:+1781986 Msa0623960:exon
TTTATTGCACTCTTCGTCCA+TGG 0.503420 4_3:-1781886 None:intergenic
GTGGAGACAAATCATAAATC+CGG 0.503730 4_3:+1782135 Msa0623960:three_prime_UTR
AAGCAAATCATAGTCATGTA+CGG 0.515890 4_3:+1778349 None:intergenic
TATCCGGATACTCCTTGTGC+TGG 0.524751 4_3:-1782324 None:intergenic
AAAGTTGAACATGATTTCCA+TGG 0.534777 4_3:+1781869 Msa0623960:CDS
CAGCTTCTTCTAGCATATGT+AGG 0.535315 4_3:-1782161 None:intergenic
TTATACAATTAAATAAGGTA+TGG 0.535450 4_3:-1782516 None:intergenic
CGGATACTCCTTGTGCTGGT+AGG 0.539807 4_3:-1782320 None:intergenic
AGCAAATCATAGTCATGTAC+GGG 0.560544 4_3:+1778350 None:intergenic
CTACATATGCTAGAAGAAGC+TGG 0.564277 4_3:+1782162 Msa0623960:three_prime_UTR
GGAATTAGAGATAATGACAA+TGG 0.586826 4_3:+1782242 Msa0623960:three_prime_UTR
AAGCAAATAAGGAATAGCGT+TGG 0.633491 4_3:+1781983 Msa0623960:exon
CAAATGTGCTTCATATTATG+CGG 0.636821 4_3:+1782113 Msa0623960:three_prime_UTR
ACTCGATTCCTACCAGCACA+AGG 0.647018 4_3:+1782312 Msa0623960:three_prime_UTR
ATGTGCTTCATATTATGCGG+TGG 0.654013 4_3:+1782116 Msa0623960:three_prime_UTR
AATTCTTGGACAAACAAGAG+AGG 0.663272 4_3:+1782221 Msa0623960:three_prime_UTR
ATATACTGCAATGTGGTATG+GGG 0.700671 4_3:-1782190 None:intergenic
GGAAAACATATACTGCAATG+TGG 0.704265 4_3:-1782197 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTATTTTAAAAATTT+AGG - chr4_3:1780687-1780706 None:intergenic 0.0%
!! AAAAATTGTCATTATAATAA+TGG - chr4_3:1778652-1778671 None:intergenic 10.0%
!!! AAAATAAGTATTTTTATTCT+AGG + chr4_3:1778706-1778725 Msa0623960:intron 10.0%
!!! AAAAAATTCTAATTTTTTTC+AGG + chr4_3:1779087-1779106 Msa0623960:intron 10.0%
!!! AATCTTAATTATTTTATAGT+TGG + chr4_3:1779303-1779322 Msa0623960:intron 10.0%
!!! ATCTTAATTATTTTATAGTT+GGG + chr4_3:1779304-1779323 Msa0623960:intron 10.0%
!! CAATTTATAGAAAAAAATTA+TGG - chr4_3:1780052-1780071 None:intergenic 10.0%
!!! TTTTCTATAAATTGATTATT+AGG + chr4_3:1780058-1780077 Msa0623960:intron 10.0%
!!! TTTTGTATTTTAATTAGTTA+GGG - chr4_3:1780642-1780661 None:intergenic 10.0%
!!! TTTTGTATTTTAATTAGTTA+GGG + chr4_3:1780642-1780661 Msa0623960:intron 10.0%
!!! TTTCTTATGTATTTTTTTAT+TGG + chr4_3:1781329-1781348 Msa0623960:intron 10.0%
!! GAATATATCAACAAATATAA+TGG - chr4_3:1778739-1778758 None:intergenic 15.0%
!!! ATTTTGTTGTCTTTTTATTA+TGG + chr4_3:1778759-1778778 Msa0623960:intron 15.0%
!!! TTTTGTTGTCTTTTTATTAT+GGG + chr4_3:1778760-1778779 Msa0623960:intron 15.0%
!!! TGAAGATTGAAAATTTTATA+GGG - chr4_3:1778877-1778896 None:intergenic 15.0%
!!! ATTCATATTTAATTTGTGTT+TGG + chr4_3:1779062-1779081 Msa0623960:intron 15.0%
!!! ACATAAATTAAGTTGAAAAT+AGG + chr4_3:1779160-1779179 Msa0623960:intron 15.0%
!!! CATAAATTAAGTTGAAAATA+GGG + chr4_3:1779161-1779180 Msa0623960:intron 15.0%
!! GAAAAATTGACATATTTATA+GGG + chr4_3:1779221-1779240 Msa0623960:intron 15.0%
!! TAATAATATTCAATTGCAAA+TGG + chr4_3:1780257-1780276 Msa0623960:intron 15.0%
!! AATAATATTCAATTGCAAAT+GGG + chr4_3:1780258-1780277 Msa0623960:intron 15.0%
!!! TTTGTATTTTAATTAGTTAG+GGG - chr4_3:1780643-1780662 None:intergenic 15.0%
!!! GTTTTGTATTTTAATTAGTT+AGG - chr4_3:1780641-1780660 None:intergenic 15.0%
!!! GTTTTGTATTTTAATTAGTT+AGG + chr4_3:1780641-1780660 Msa0623960:intron 15.0%
!!! TTTGTATTTTAATTAGTTAG+GGG + chr4_3:1780643-1780662 Msa0623960:intron 15.0%
!!! TTTTTTCAATGATTTAACAT+TGG - chr4_3:1781065-1781084 None:intergenic 15.0%
!! AAAAAAAATTCTTAGTCTAA+AGG + chr4_3:1781079-1781098 Msa0623960:intron 15.0%
!! AGTATACTATTGTTAAAAAA+AGG - chr4_3:1781366-1781385 None:intergenic 15.0%
!!! TGTTATTTAAACTTAAGATA+AGG + chr4_3:1781386-1781405 Msa0623960:intron 15.0%
!! TAAAATATATAAAGCTACAA+GGG - chr4_3:1781692-1781711 None:intergenic 15.0%
!! ATAAAATATATAAAGCTACA+AGG - chr4_3:1781693-1781712 None:intergenic 15.0%
!! TAATGAAATAAGATCTAATT+TGG + chr4_3:1781754-1781773 Msa0623960:intron 15.0%
!! TTATACAATTAAATAAGGTA+TGG - chr4_3:1782519-1782538 None:intergenic 15.0%
!!! TTATTATGGGTTTTTTTTGT+AGG + chr4_3:1778773-1778792 Msa0623960:intron 20.0%
!!! TATTATGGGTTTTTTTTGTA+GGG + chr4_3:1778774-1778793 Msa0623960:intron 20.0%
!! GAAAAGTTGACATATTTATA+GGG - chr4_3:1778817-1778836 None:intergenic 20.0%
!!! AGTTGAAAGAAAATTTTAGA+GGG - chr4_3:1778848-1778867 None:intergenic 20.0%
!!! AAGTTGAAAGAAAATTTTAG+AGG - chr4_3:1778849-1778868 None:intergenic 20.0%
!!! GTGAAGATTGAAAATTTTAT+AGG - chr4_3:1778878-1778897 None:intergenic 20.0%
!!! AAATTTTCAATCTTCACTTT+TGG + chr4_3:1778881-1778900 Msa0623960:intron 20.0%
!!! TATGAGTTTACTTTAAAAGA+GGG - chr4_3:1778908-1778927 None:intergenic 20.0%
!!! ATATGAGTTTACTTTAAAAG+AGG - chr4_3:1778909-1778928 None:intergenic 20.0%
!!! TAAAATTTTCCTTCAACTTT+TGG - chr4_3:1779188-1779207 None:intergenic 20.0%
!! CGAAAAATTGACATATTTAT+AGG + chr4_3:1779220-1779239 Msa0623960:intron 20.0%
!!! AAAAGGGTTAAATATGTTTT+TGG - chr4_3:1779248-1779267 None:intergenic 20.0%
!! AATGATACGTATACAAAAAA+GGG - chr4_3:1779264-1779283 None:intergenic 20.0%
!! AAATGATACGTATACAAAAA+AGG - chr4_3:1779265-1779284 None:intergenic 20.0%
!! ACCTAACATATGATTATATA+GGG - chr4_3:1779890-1779909 None:intergenic 20.0%
!! AACCTAACATATGATTATAT+AGG - chr4_3:1779891-1779910 None:intergenic 20.0%
!! ATATAATCATATGTTAGGTT+AGG + chr4_3:1779891-1779910 Msa0623960:intron 20.0%
!!! GATTACTTTGTTATTTCATT+TGG + chr4_3:1779983-1780002 Msa0623960:intron 20.0%
!!! ATGAATGTTTTTGAGTATTA+TGG - chr4_3:1780229-1780248 None:intergenic 20.0%
!!! TGGTAGTTGTATTAAATTTT+TGG + chr4_3:1780382-1780401 Msa0623960:intron 20.0%
!!! TTGTATTTTAATTAGTTAGG+GGG - chr4_3:1780644-1780663 None:intergenic 20.0%
!!! TTGTATTTTAATTAGTTAGG+GGG + chr4_3:1780644-1780663 Msa0623960:intron 20.0%
!! AGAATGAGCATAATATTTAA+GGG + chr4_3:1780825-1780844 Msa0623960:intron 20.0%
!!! GAATATTAGTTTTGAAAACT+GGG - chr4_3:1780885-1780904 None:intergenic 20.0%
!!! TGAATATTAGTTTTGAAAAC+TGG - chr4_3:1780886-1780905 None:intergenic 20.0%
!!! TGATTTTCTCTTTTTTCATA+TGG + chr4_3:1780983-1781002 Msa0623960:intron 20.0%
!! AATACACATTTATACTACAT+GGG + chr4_3:1781478-1781497 Msa0623960:intron 20.0%
!!! TGAATTGTTCTAGAAATAAT+AGG + chr4_3:1781656-1781675 Msa0623960:intron 20.0%
!! AAAATATATAAAGCTACAAG+GGG - chr4_3:1781691-1781710 None:intergenic 20.0%
!!! GTTGAGTTTTTTTTTTTTTG+TGG + chr4_3:1781722-1781741 Msa0623960:intron 20.0%
!! GTCAATAAACAAAAATTATG+TGG + chr4_3:1781920-1781939 Msa0623960:CDS 20.0%
!! TAAAAAAGAAATTGTGATGT+CGG + chr4_3:1782265-1782284 Msa0623960:three_prime_UTR 20.0%
! TTATGCAATTTCACATGAAA+TGG - chr4_3:1778458-1778477 None:intergenic 25.0%
! CAAAAACAATGTCATATATG+TGG + chr4_3:1778579-1778598 Msa0623960:intron 25.0%
!! TGTAGGGTTAAATATGTTTT+TGG + chr4_3:1778790-1778809 Msa0623960:intron 25.0%
! CGAAAAGTTGACATATTTAT+AGG - chr4_3:1778818-1778837 None:intergenic 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + chr4_3:1779189-1779208 Msa0623960:intron 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + chr4_3:1779190-1779209 Msa0623960:intron 25.0%
! TCTAAGAGTTCAGTATTTAT+AGG - chr4_3:1779747-1779766 None:intergenic 25.0%
! TCCCTATATAATCATATGTT+AGG + chr4_3:1779886-1779905 Msa0623960:intron 25.0%
! TACATGAACAACTAATTACA+TGG - chr4_3:1779934-1779953 None:intergenic 25.0%
!!! TTGCAACTTTTGAAAAGATA+GGG - chr4_3:1780427-1780446 None:intergenic 25.0%
!!! ATTGCAACTTTTGAAAAGAT+AGG - chr4_3:1780428-1780447 None:intergenic 25.0%
!! CTTTTCAAAAGTTGCAATTT+TGG + chr4_3:1780430-1780449 Msa0623960:intron 25.0%
! GAGAATGAGCATAATATTTA+AGG + chr4_3:1780824-1780843 Msa0623960:intron 25.0%
! TTAAACTTAAGATAAGGAAG+TGG + chr4_3:1781392-1781411 Msa0623960:intron 25.0%
! GAATACACATTTATACTACA+TGG + chr4_3:1781477-1781496 Msa0623960:intron 25.0%
! GCAATTATTAAACACAAAAG+AGG + chr4_3:1781534-1781553 Msa0623960:intron 25.0%
!! TTCCTTTGGCATAAAATTTT+CGG - chr4_3:1781802-1781821 None:intergenic 25.0%
! AAACAAAAATTATGTGGACA+TGG + chr4_3:1781926-1781945 Msa0623960:CDS 25.0%
! CAAACTTCAAAGATGTATAT+TGG - chr4_3:1782066-1782085 None:intergenic 25.0%
!! GTATATGTTTTCCAAATTCT+TGG + chr4_3:1782207-1782226 Msa0623960:three_prime_UTR 25.0%
! AAAAAAGAAATTGTGATGTC+GGG + chr4_3:1782266-1782285 Msa0623960:three_prime_UTR 25.0%
! ATTTGTGTCACAATAATCAT+TGG - chr4_3:1782411-1782430 None:intergenic 25.0%
TGTGAAATTGCATAAGTGTT+AGG + chr4_3:1778463-1778482 Msa0623960:intron 30.0%
GTGAAATTGCATAAGTGTTA+GGG + chr4_3:1778464-1778483 Msa0623960:intron 30.0%
! GGGACTTCTAGTTAAAATTA+TGG + chr4_3:1778525-1778544 Msa0623960:intron 30.0%
GAGTAATGATGTTAATGTCA+AGG - chr4_3:1778614-1778633 None:intergenic 30.0%
TGACATTAACATCATTACTC+AGG + chr4_3:1778614-1778633 Msa0623960:intron 30.0%
TTAACATCATTACTCAGGTA+AGG + chr4_3:1778619-1778638 Msa0623960:intron 30.0%
! CTTTTCAATAAACGTGTCTT+TGG + chr4_3:1779454-1779473 Msa0623960:intron 30.0%
! ATAATTTGCATCTTCCAGTT+GGG - chr4_3:1779544-1779563 None:intergenic 30.0%
!!! CTTTGTTTTGTAAAGCGATA+AGG + chr4_3:1779633-1779652 Msa0623960:intron 30.0%
AACCAATTTCCCTAAAACTT+AGG + chr4_3:1779711-1779730 Msa0623960:intron 30.0%
ACCAATTTCCCTAAAACTTA+GGG + chr4_3:1779712-1779731 Msa0623960:intron 30.0%
! GGATAAAAAAGCATGAGAAA+AGG - chr4_3:1779869-1779888 None:intergenic 30.0%
ACCTAGTTTAACCATTCAAT+CGG + chr4_3:1780116-1780135 Msa0623960:intron 30.0%
TTTAACCATTCAATCGGTTA+GGG + chr4_3:1780122-1780141 Msa0623960:intron 30.0%
! ACAAGCATGTGATATTTTGT+TGG + chr4_3:1780201-1780220 Msa0623960:intron 30.0%
ACTCAAAAACATTCATGTCA+TGG + chr4_3:1780233-1780252 Msa0623960:intron 30.0%
AATATTCAATTGCAAATGGG+TGG + chr4_3:1780261-1780280 Msa0623960:intron 30.0%
ACTATATAGCATACCTCTTA+CGG - chr4_3:1780324-1780343 None:intergenic 30.0%
!!! TTTTGGCTTAATTGCACTTT+TGG + chr4_3:1780399-1780418 Msa0623960:intron 30.0%
!! TGCAACTTTTGAAAAGATAG+GGG - chr4_3:1780426-1780445 None:intergenic 30.0%
! TTTAGGCTTAATTGCACTTT+TGG - chr4_3:1780670-1780689 None:intergenic 30.0%
!!! TTAGTTTTGAAAACTGGGTT+TGG - chr4_3:1780880-1780899 None:intergenic 30.0%
! TATCATTGATGACAAGAACA+TGG - chr4_3:1780954-1780973 None:intergenic 30.0%
TTACGTAGAAGCAACATTTA+TGG + chr4_3:1781146-1781165 Msa0623960:intron 30.0%
!!! TGCTTCTTTTTTTCTGTTGA+AGG + chr4_3:1781169-1781188 Msa0623960:intron 30.0%
!!! CTTTTTTTCTGTTGAAGGAA+TGG + chr4_3:1781174-1781193 Msa0623960:intron 30.0%
TAATTTCACAACTGGTCATT+TGG + chr4_3:1781203-1781222 Msa0623960:intron 30.0%
! CATTTGGTGAAAACCATTTA+TGG + chr4_3:1781219-1781238 Msa0623960:intron 30.0%
AAATAAGACCGAATGATTGA+TGG - chr4_3:1781595-1781614 None:intergenic 30.0%
! TCCTTTGGCATAAAATTTTC+GGG - chr4_3:1781801-1781820 None:intergenic 30.0%
TTATGGTATACAACTTCCTT+TGG - chr4_3:1781816-1781835 None:intergenic 30.0%
ACAGCTTATCATGTATGTTA+TGG - chr4_3:1781833-1781852 None:intergenic 30.0%
TGGAAATCATGTTCAACTTT+GGG - chr4_3:1781869-1781888 None:intergenic 30.0%
ATGGAAATCATGTTCAACTT+TGG - chr4_3:1781870-1781889 None:intergenic 30.0%
AAAGTTGAACATGATTTCCA+TGG + chr4_3:1781869-1781888 Msa0623960:CDS 30.0%
! TATTCCTTATTTGCTTCGTT+TGG - chr4_3:1781979-1781998 None:intergenic 30.0%
CAAATGTGCTTCATATTATG+CGG + chr4_3:1782113-1782132 Msa0623960:three_prime_UTR 30.0%
GGAATTAGAGATAATGACAA+TGG + chr4_3:1782242-1782261 Msa0623960:three_prime_UTR 30.0%
AATGCTCTCATCAAATGATG+AGG - chr4_3:1778417-1778436 None:intergenic 35.0%
!! TCATTTGATGAGAGCATTCA+AGG + chr4_3:1778419-1778438 Msa0623960:intron 35.0%
TGATCCAATGATAGCATTAC+AGG + chr4_3:1778503-1778522 Msa0623960:intron 35.0%
GATCCAATGATAGCATTACA+GGG + chr4_3:1778504-1778523 Msa0623960:intron 35.0%
ATCCAATGATAGCATTACAG+GGG + chr4_3:1778505-1778524 Msa0623960:intron 35.0%
AAATAGGGACCAAAAGTTGA+AGG + chr4_3:1779176-1779195 Msa0623960:intron 35.0%
AGACTACAACTTGAGCATAA+AGG + chr4_3:1779330-1779349 Msa0623960:intron 35.0%
ACAAATATCTTCAACCCAAC+TGG + chr4_3:1779527-1779546 Msa0623960:intron 35.0%
! CATAATTTGCATCTTCCAGT+TGG - chr4_3:1779545-1779564 None:intergenic 35.0%
TTGTAAAGCGATAAGGTCAT+AGG + chr4_3:1779640-1779659 Msa0623960:intron 35.0%
TACCTCACATTGCAAAGTAT+AGG + chr4_3:1779677-1779696 Msa0623960:intron 35.0%
!!! ACCCTAAGTTTTAGGGAAAT+TGG - chr4_3:1779716-1779735 None:intergenic 35.0%
! AACCATGATTTTCGAAGGAT+TGG + chr4_3:1779798-1779817 Msa0623960:intron 35.0%
TTCCAATCCTTCGAAAATCA+TGG - chr4_3:1779803-1779822 None:intergenic 35.0%
! ATCATCGATTGATGATACCT+TGG - chr4_3:1779846-1779865 None:intergenic 35.0%
AGGTCAAGACAACAAAGTTA+GGG - chr4_3:1780100-1780119 None:intergenic 35.0%
TAGGTCAAGACAACAAAGTT+AGG - chr4_3:1780101-1780120 None:intergenic 35.0%
ACCGATTGAATGGTTAAACT+AGG - chr4_3:1780120-1780139 None:intergenic 35.0%
GTTTAACCATTCAATCGGTT+AGG + chr4_3:1780121-1780140 Msa0623960:intron 35.0%
!!! TTAGGGTTTTGACTTCTTGT+GGG + chr4_3:1780139-1780158 Msa0623960:intron 35.0%
CATATTGCTGGTGATAACAA+AGG + chr4_3:1780175-1780194 Msa0623960:intron 35.0%
! TACGGTATTTAGGATCAACA+AGG - chr4_3:1780306-1780325 None:intergenic 35.0%
TTGATCCTAAATACCGTAAG+AGG + chr4_3:1780308-1780327 Msa0623960:intron 35.0%
!! GCAACTTTTGAAAAGATAGG+GGG - chr4_3:1780425-1780444 None:intergenic 35.0%
TGCCAAAGATCCAATACATA+GGG - chr4_3:1780614-1780633 None:intergenic 35.0%
! TATTGGATCTTTGGCAGTTT+TGG + chr4_3:1780490-1780509 Msa0623960:intron 35.0%
! TATTGGATCTTTGGCAGTTT+TGG - chr4_3:1780490-1780509 None:intergenic 35.0%
TGCCAAAGATCCAATACATA+AGG + chr4_3:1780614-1780633 Msa0623960:intron 35.0%
GCCAAAGATCCAATACATAA+GGG + chr4_3:1780615-1780634 Msa0623960:intron 35.0%
CCAAAGATCCAATACATAAG+GGG + chr4_3:1780616-1780635 Msa0623960:intron 35.0%
! ACAGTTTGAGATTTGTCCTA+CGG + chr4_3:1780721-1780740 Msa0623960:intron 35.0%
!!! TCGATGACATGGGTTTTTTA+CGG - chr4_3:1780806-1780825 None:intergenic 35.0%
CTACGTAAAATTGCATAGCT+TGG - chr4_3:1781134-1781153 None:intergenic 35.0%
!!! GGTGCTTTTAATTTCACAAC+TGG + chr4_3:1781195-1781214 Msa0623960:intron 35.0%
! CAGTAAAAGCACACCATAAA+TGG - chr4_3:1781235-1781254 None:intergenic 35.0%
! ACTACATGGGTGCTATAAAT+TGG + chr4_3:1781491-1781510 Msa0623960:intron 35.0%
! CTACATGGGTGCTATAAATT+GGG + chr4_3:1781492-1781511 Msa0623960:intron 35.0%
!! ATCTAATTTGGTGTTGATGC+AGG + chr4_3:1781766-1781785 Msa0623960:intron 35.0%
CATACATGATAAGCTGTTGT+TGG + chr4_3:1781836-1781855 Msa0623960:CDS 35.0%
AGAACCAAACGAAGCAAATA+AGG + chr4_3:1781972-1781991 Msa0623960:CDS 35.0%
AAGCAAATAAGGAATAGCGT+TGG + chr4_3:1781983-1782002 Msa0623960:exon 35.0%
GTGGAGACAAATCATAAATC+CGG + chr4_3:1782135-1782154 Msa0623960:three_prime_UTR 35.0%
TTCTAGCATATGTAGGTATC+CGG - chr4_3:1782157-1782176 None:intergenic 35.0%
ATATACTGCAATGTGGTATG+GGG - chr4_3:1782193-1782212 None:intergenic 35.0%
CATATACTGCAATGTGGTAT+GGG - chr4_3:1782194-1782213 None:intergenic 35.0%
ACATATACTGCAATGTGGTA+TGG - chr4_3:1782195-1782214 None:intergenic 35.0%
GGAAAACATATACTGCAATG+TGG - chr4_3:1782200-1782219 None:intergenic 35.0%
! CTCTTGTTTGTCCAAGAATT+TGG - chr4_3:1782221-1782240 None:intergenic 35.0%
AATTCTTGGACAAACAAGAG+AGG + chr4_3:1782221-1782240 Msa0623960:three_prime_UTR 35.0%
!!! GTCTTTTTCTGGTTTCTATC+CGG - chr4_3:1782343-1782362 None:intergenic 35.0%
ATTGCTACAGATGTCTGGTT+TGG - chr4_3:1778387-1778406 None:intergenic 40.0%
TTCCCTAAAACTTAGGGTCA+TGG + chr4_3:1779718-1779737 Msa0623960:intron 40.0%
! GTCCATGACCCTAAGTTTTA+GGG - chr4_3:1779723-1779742 None:intergenic 40.0%
! AAAAACACCTTTTGACGAGC+AGG + chr4_3:1779766-1779785 Msa0623960:intron 40.0%
! GGCAAAACCATGATTTTCGA+AGG + chr4_3:1779793-1779812 Msa0623960:intron 40.0%
ATGATACCTTGGTTTCCGAA+TGG - chr4_3:1779835-1779854 None:intergenic 40.0%
CAAAACCCTAACCGATTGAA+TGG - chr4_3:1780130-1780149 None:intergenic 40.0%
!!! GTTAGGGTTTTGACTTCTTG+TGG + chr4_3:1780138-1780157 Msa0623960:intron 40.0%
TAGGATCAACAAGGTTGTGA+AGG - chr4_3:1780297-1780316 None:intergenic 40.0%
GCATACCTCTTACGGTATTT+AGG - chr4_3:1780316-1780335 None:intergenic 40.0%
CCAAAGATCCAATACATAGG+GGG - chr4_3:1780484-1780503 None:intergenic 40.0%
GCCAAAGATCCAATACATAG+GGG - chr4_3:1780485-1780504 None:intergenic 40.0%
CTGCCAAAGATCCAATACAT+AGG - chr4_3:1780613-1780632 None:intergenic 40.0%
! TTAAGTCAAAAGTGGCCTTG+GGG - chr4_3:1780517-1780536 None:intergenic 40.0%
CTTAAGTCAAAAGTGGCCTT+GGG - chr4_3:1780518-1780537 None:intergenic 40.0%
ACTTAAGTCAAAAGTGGCCT+TGG - chr4_3:1780519-1780538 None:intergenic 40.0%
CACCTCACTTAAGTCAAAAG+TGG - chr4_3:1780525-1780544 None:intergenic 40.0%
AGCGAAGACTAAGTCAAAAG+TGG + chr4_3:1780575-1780594 Msa0623960:intron 40.0%
ACTAAGTCAAAAGTGGCCTT+GGG + chr4_3:1780582-1780601 Msa0623960:intron 40.0%
CCCCTTATGTATTGGATCTT+TGG - chr4_3:1780619-1780638 None:intergenic 40.0%
CAAAACAGCCCCTTATGTAT+TGG - chr4_3:1780627-1780646 None:intergenic 40.0%
CAACAACTGATATTGACCGT+AGG - chr4_3:1780740-1780759 None:intergenic 40.0%
!!! GACATGGGTTTTTTACGGTA+TGG - chr4_3:1780801-1780820 None:intergenic 40.0%
ATGCTCATTCTCGATGACAT+GGG - chr4_3:1780816-1780835 None:intergenic 40.0%
TATGCTCATTCTCGATGACA+TGG - chr4_3:1780817-1780836 None:intergenic 40.0%
TAATTATGCCACCCACATGA+GGG - chr4_3:1781281-1781300 None:intergenic 40.0%
ATAATTATGCCACCCACATG+AGG - chr4_3:1781282-1781301 None:intergenic 40.0%
TAGCGCACTTTCTCTTCATT+TGG - chr4_3:1781431-1781450 None:intergenic 40.0%
! GCCCGAAAATTTTATGCCAA+AGG + chr4_3:1781797-1781816 Msa0623960:CDS 40.0%
TTTATTGCACTCTTCGTCCA+TGG - chr4_3:1781889-1781908 None:intergenic 40.0%
! CAAATAAGGAATAGCGTTGG+TGG + chr4_3:1781986-1782005 Msa0623960:exon 40.0%
ATGTGCTTCATATTATGCGG+TGG + chr4_3:1782116-1782135 Msa0623960:three_prime_UTR 40.0%
CAGCTTCTTCTAGCATATGT+AGG - chr4_3:1782164-1782183 None:intergenic 40.0%
CTACATATGCTAGAAGAAGC+TGG + chr4_3:1782162-1782181 Msa0623960:three_prime_UTR 40.0%
! ACTCTGTTGCTGTCTTTTTC+TGG - chr4_3:1782354-1782373 None:intergenic 40.0%
TCGTCATTGCTACAGATGTC+TGG - chr4_3:1778392-1778411 None:intergenic 45.0%
!! GAGAGCATTCAAGGTGTTGT+TGG + chr4_3:1778428-1778447 Msa0623960:intron 45.0%
GTCCCCTGTAATGCTATCAT+TGG - chr4_3:1778510-1778529 None:intergenic 45.0%
ACTTCAATGCTGTCATGCGT+AGG - chr4_3:1779369-1779388 None:intergenic 45.0%
GGCCTATACTTTGCAATGTG+AGG - chr4_3:1779682-1779701 None:intergenic 45.0%
!! GGGAAATTGGTTCGTGATGT+AGG - chr4_3:1779703-1779722 None:intergenic 45.0%
! CGTCCATGACCCTAAGTTTT+AGG - chr4_3:1779724-1779743 None:intergenic 45.0%
!!! GGTTTTGACTTCTTGTGGGA+TGG + chr4_3:1780143-1780162 Msa0623960:intron 45.0%
! TTTGACTTCTTGTGGGATGG+AGG + chr4_3:1780146-1780165 Msa0623960:intron 45.0%
TTGACTTCTTGTGGGATGGA+GGG + chr4_3:1780147-1780166 Msa0623960:intron 45.0%
!! TGGAGGGAGTATCATATTGC+TGG + chr4_3:1780163-1780182 Msa0623960:intron 45.0%
GAATAGCACACGATACATGC+TGG + chr4_3:1780362-1780381 Msa0623960:intron 45.0%
CAAAACAGCCCCCTATGTAT+TGG + chr4_3:1780473-1780492 Msa0623960:intron 45.0%
CCCCCTATGTATTGGATCTT+TGG + chr4_3:1780481-1780500 Msa0623960:intron 45.0%
! TAAGTCAAAAGTGGCCTTGG+GGG - chr4_3:1780584-1780603 None:intergenic 45.0%
!!! GGCCACTTTTGACTTAAGTG+AGG + chr4_3:1780520-1780539 Msa0623960:intron 45.0%
GACTAAGTCAAAAGTGGCCT+TGG + chr4_3:1780581-1780600 Msa0623960:intron 45.0%
! CTAAGTCAAAAGTGGCCTTG+GGG + chr4_3:1780583-1780602 Msa0623960:intron 45.0%
! TAAGTCAAAAGTGGCCTTGG+GGG + chr4_3:1780584-1780603 Msa0623960:intron 45.0%
TTCATATGGTTCATGCGCAG+TGG + chr4_3:1780997-1781016 Msa0623960:intron 45.0%
AGCTTGGTACACGAAAACGA+TGG - chr4_3:1781118-1781137 None:intergenic 45.0%
GCATGTGGCCATCAATCATT+CGG + chr4_3:1781584-1781603 Msa0623960:intron 45.0%
!! AGGAATAGCGTTGGTGGAAA+AGG + chr4_3:1781992-1782011 Msa0623960:exon 45.0%
!!! AAAAAATTCTAAATTTTTTT+TGG - chr4_3:1778994-1779013 None:intergenic 5.0%
!!! ATGAATTTTTATATTTTTTA+CGG + chr4_3:1779033-1779052 Msa0623960:intron 5.0%
!! AAGATTAATATTAAATATAA+TGG - chr4_3:1779289-1779308 None:intergenic 5.0%
!!! ATAAATAAATTTTTAAAATG+AGG - chr4_3:1779484-1779503 None:intergenic 5.0%
TTGGAACAACCAGCGCCATT+CGG + chr4_3:1779817-1779836 Msa0623960:intron 50.0%
TGCCACATGTCGACCATTGT+GGG + chr4_3:1780543-1780562 Msa0623960:intron 50.0%
! TGACTTAGTCTTCGCTGACG+TGG - chr4_3:1780571-1780590 None:intergenic 50.0%
CATGCATGCAGTAGCACTAG+TGG + chr4_3:1781032-1781051 Msa0623960:intron 50.0%
ACACAAGAGTGAGCTGCATG+TGG + chr4_3:1781569-1781588 Msa0623960:intron 50.0%
! GCGTTGGTGGAAAAGGTGAA+AGG + chr4_3:1781999-1782018 Msa0623960:intron 50.0%
ACTCGATTCCTACCAGCACA+AGG + chr4_3:1782312-1782331 Msa0623960:three_prime_UTR 50.0%
!! CTACCAGCACAAGGAGTATC+CGG + chr4_3:1782321-1782340 Msa0623960:three_prime_UTR 50.0%
TATCCGGATACTCCTTGTGC+TGG - chr4_3:1782327-1782346 None:intergenic 50.0%
! ACCTTTTGACGAGCAGGCGA+AGG + chr4_3:1779772-1779791 Msa0623960:intron 55.0%
!! TTTGGCAGTTTTGGCCCCCA+AGG + chr4_3:1780499-1780518 Msa0623960:intron 55.0%
GTGCCACATGTCGACCATTG+TGG + chr4_3:1780542-1780561 Msa0623960:intron 55.0%
CACCCACAATGGTCGACATG+TGG - chr4_3:1780548-1780567 None:intergenic 55.0%
!! TTTGGCAGTTTTGGCCCCCA+AGG - chr4_3:1780499-1780518 None:intergenic 55.0%
CATGCAGTAGCACTAGTGGC+TGG + chr4_3:1781036-1781055 Msa0623960:intron 55.0%
! TGATGATGCCAGCCCTCATG+TGG + chr4_3:1781266-1781285 Msa0623960:intron 55.0%
GATGATGCCAGCCCTCATGT+GGG + chr4_3:1781267-1781286 Msa0623960:intron 55.0%
! CGGATACTCCTTGTGCTGGT+AGG - chr4_3:1782323-1782342 None:intergenic 55.0%
GCCTTCGCCTGCTCGTCAAA+AGG - chr4_3:1779776-1779795 None:intergenic 60.0%
CCTTGGTTTCCGAATGGCGC+TGG - chr4_3:1779829-1779848 None:intergenic 60.0%
CCAGCGCCATTCGGAAACCA+AGG + chr4_3:1779826-1779845 Msa0623960:intron 60.0%
TATGCCACCCACATGAGGGC+TGG - chr4_3:1781277-1781296 None:intergenic 60.0%
CGCTGACGTGGCACCCACAA+TGG - chr4_3:1780559-1780578 None:intergenic 65.0%
GATGCCAGCCCTCATGTGGG+TGG + chr4_3:1781270-1781289 Msa0623960:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 1778364 1782541 1778364 ID=Msa0623960;Name=Msa0623960
chr4_3 mRNA 1778364 1782541 1778364 ID=Msa0623960-mRNA-1;Parent=Msa0623960;Name=Msa0623960-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0.5|0.66|0.66|0|0|3|460|70
chr4_3 exon 1778364 1778371 1778364 ID=Msa0623960-mRNA-1:exon:472;Parent=Msa0623960-mRNA-1
chr4_3 exon 1781788 1782013 1781788 ID=Msa0623960-mRNA-1:exon:473;Parent=Msa0623960-mRNA-1
chr4_3 exon 1782103 1782541 1782103 ID=Msa0623960-mRNA-1:exon:474;Parent=Msa0623960-mRNA-1
chr4_3 CDS 1778364 1778371 1778364 ID=Msa0623960-mRNA-1:cds;Parent=Msa0623960-mRNA-1
chr4_3 CDS 1781788 1781992 1781788 ID=Msa0623960-mRNA-1:cds;Parent=Msa0623960-mRNA-1
chr4_3 three_prime_UTR 1781993 1782013 1781993 ID=Msa0623960-mRNA-1:three_prime_utr;Parent=Msa0623960-mRNA-1
chr4_3 three_prime_UTR 1782103 1782541 1782103 ID=Msa0623960-mRNA-1:three_prime_utr;Parent=Msa0623960-mRNA-1
Gene Sequence

>Msa0623960

ATGTACGGGTTCAACATGCCCGAAAATTTTATGCCAAAGGAAGTTGTATACCATAACATACATGATAAGCTGTTGTTGGATTCTATCCCAAAGTTGAACATGATTTCCATGGACGAAGAGTGCAATAAACTTCTCATAGAGTCAATAAACAAAAATTATGTGGACATGGCTGATTATTTGATGAGTATCAACAGAACCAAACGAAGCAAATAA
Protein sequence

>Msa0623960

MYGFNMPENFMPKEVVYHNIHDKLLLDSIPKLNMISMDEECNKLLIESINKNYVDMADYLMSINRTKRSK*