AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1404210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404210 MtrunA17_Chr8g0375301 58.571 70 24 1 50 114 30 99 7.70e-20 83.2
Msa1404210 MtrunA17_Chr5g0433921 50.820 61 25 1 50 105 30 90 3.87e-14 63.2
Msa1404210 MtrunA17_Chr6g0480631 44.286 70 34 1 50 114 33 102 4.39e-14 67.0
Msa1404210 MtrunA17_Chr3g0109901 44.286 70 34 1 50 114 30 99 3.07e-13 64.7
Msa1404210 MtrunA17_Chr4g0038791 71.053 38 11 0 52 89 1 38 4.04e-12 56.6
Msa1404210 MtrunA17_Chr4g0038801 69.048 42 11 1 50 91 19 58 1.00e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404210 AT2G02010.1 48.571 70 31 1 50 114 30 99 1.28e-15 71.6
Msa1404210 AT2G02010.2 48.571 70 31 1 50 114 82 151 2.99e-15 70.9
Msa1404210 AT5G17330.1 47.143 70 32 1 50 114 30 99 2.48e-14 68.2
Msa1404210 AT3G17760.2 39.241 79 43 1 41 114 20 98 1.00e-13 66.2
Msa1404210 AT3G17760.1 39.241 79 43 1 41 114 20 98 1.00e-13 66.2
Msa1404210 AT1G65960.2 41.429 70 36 1 50 114 29 98 1.09e-11 60.5
Msa1404210 AT2G02000.1 41.429 70 36 1 50 114 30 99 1.92e-11 59.7

Find 52 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGTTTGTCCAAGAATT+TGG 0.102194 tig0023048:-84915 Msa1404210:intergenic
GCATACCTCTTACGGTATTT+AGG 0.220386 tig0023048:-83006 Msa1404210:intergenic
CATTTGGTGAAAACCATTTA+TGG 0.236116 tig0023048:+83912 Msa1404210:CDS
TTGGCTTATACAATTAAATA+AGG 0.257234 tig0023048:-85218 Msa1404210:intergenic
CAGTAAAAGCACACCATAAA+TGG 0.265138 tig0023048:-83925 Msa1404210:intergenic
AGAACCAAACGAAGCAAATA+AGG 0.330837 tig0023048:+84670 Msa1404210:CDS
TAATTTCACAACTGGTCATT+TGG 0.333102 tig0023048:+83896 Msa1404210:CDS
TATTCCTTATTTGCTTCGTT+TGG 0.353520 tig0023048:-84674 Msa1404210:intergenic
CGGAAATCATGTTCAACTTT+GGG 0.371042 tig0023048:-84564 Msa1404210:intergenic
TTATGGTATACAACTTCCTT+TGG 0.381162 tig0023048:-84511 Msa1404210:intergenic
TAATAATATTCAATTGCAAA+TGG 0.382878 tig0023048:+82950 Msa1404210:CDS
ATTTGTGTCACAATAATCAT+TGG 0.382968 tig0023048:-85105 Msa1404210:intergenic
CATACATGATAAGCTGTTGT+TGG 0.391560 tig0023048:+84534 Msa1404210:CDS
ACGGAAATCATGTTCAACTT+TGG 0.395439 tig0023048:-84565 Msa1404210:intergenic
TTCCAATCCTTCGAAAATCA+TGG 0.436898 tig0023048:-82493 Msa1404210:intergenic
ACAGCTTATCATGTATGTTA+TGG 0.438601 tig0023048:-84528 Msa1404210:intergenic
AGGAATAGCGTTGGTGGAAA+AGG 0.441467 tig0023048:+84690 Msa1404210:exon
ACATATACTGCAATGTGGTA+TGG 0.442915 tig0023048:-84889 Msa1404210:intergenic
TTGATCCTAAATACCGTAAG+AGG 0.445013 tig0023048:+83001 Msa1404210:CDS
CCTTGGTTTCCGAATGGCGC+TGG 0.448080 tig0023048:-82519 Msa1404210:intergenic
TTCTAGCATATGTAGGTATC+CGG 0.451200 tig0023048:-84851 Msa1404210:intergenic
TGGAGGGAGTATCATATTGC+TGG 0.461103 tig0023048:+82856 Msa1404210:five_prime_UTR
CATATACTGCAATGTGGTAT+GGG 0.464419 tig0023048:-84888 Msa1404210:intergenic
TTTGACTTCTTGTGGGATGG+AGG 0.465976 tig0023048:+82839 Msa1404210:five_prime_UTR
AATAATATTCAATTGCAAAT+GGG 0.474702 tig0023048:+82951 Msa1404210:CDS
TTGGAACAACCAGCGCCATT+CGG 0.482488 tig0023048:+82510 Msa1404210:five_prime_UTR
TTGACTTCTTGTGGGATGGA+GGG 0.483356 tig0023048:+82840 Msa1404210:five_prime_UTR
CTACCAGCACAAGGAGTATC+CGG 0.486689 tig0023048:+85018 Msa1404210:three_prime_UTR
CAAATAAGGAATAGCGTTGG+TGG 0.493624 tig0023048:+84684 Msa1404210:exon
GTGGAGACAAATCATAAATC+CGG 0.503730 tig0023048:+84832 Msa1404210:three_prime_UTR
ACTATATAGCATACCTCTTA+CGG 0.505460 tig0023048:-83014 Msa1404210:intergenic
TATCCGGATACTCCTTGTGC+TGG 0.524751 tig0023048:-85021 Msa1404210:intergenic
CAGCTTCTTCTAGCATATGT+AGG 0.535315 tig0023048:-84858 Msa1404210:intergenic
TTATACAATTAAATAAGGTA+TGG 0.535450 tig0023048:-85213 Msa1404210:intergenic
CGGATACTCCTTGTGCTGGT+AGG 0.539807 tig0023048:-85017 Msa1404210:intergenic
TAGGATCAACAAGGTTGTGA+AGG 0.549552 tig0023048:-82987 Msa1404210:intergenic
TTTATTGCACTCTTCGTCCA+CGG 0.561642 tig0023048:-84584 Msa1404210:intergenic
CTACATATGCTAGAAGAAGC+TGG 0.564277 tig0023048:+84859 Msa1404210:three_prime_UTR
ATGATACCTTGGTTTCCGAA+TGG 0.578017 tig0023048:-82525 Msa1404210:intergenic
GGAATTAGAGATAATGACAA+TGG 0.586826 tig0023048:+84939 Msa1404210:three_prime_UTR
AATATTCAATTGCAAATGGG+TGG 0.601700 tig0023048:+82954 Msa1404210:CDS
AAAGTTGAACATGATTTCCG+TGG 0.617602 tig0023048:+84567 Msa1404210:CDS
CATATTGCTGGTGATAACAA+AGG 0.619643 tig0023048:+82868 Msa1404210:five_prime_UTR
AAGCAAATAAGGAATAGCGT+TGG 0.633491 tig0023048:+84681 Msa1404210:exon
CAAATGTGCTTCATATTATG+CGG 0.636821 tig0023048:+84810 Msa1404210:three_prime_UTR
TACGGTATTTAGGATCAACA+AGG 0.643427 tig0023048:-82996 Msa1404210:intergenic
ACTCGATTCCTACCAGCACA+AGG 0.647018 tig0023048:+85009 Msa1404210:three_prime_UTR
ATGTGCTTCATATTATGCGG+TGG 0.654013 tig0023048:+84813 Msa1404210:three_prime_UTR
AATTCTTGGACAAACAAGAG+AGG 0.663272 tig0023048:+84918 Msa1404210:three_prime_UTR
CCAGCGCCATTCGGAAACCA+AGG 0.663710 tig0023048:+82519 Msa1404210:five_prime_UTR
ATATACTGCAATGTGGTATG+GGG 0.700671 tig0023048:-84887 Msa1404200:intergenic
GGAAAACATATACTGCAATG+TGG 0.704265 tig0023048:-84894 Msa1404200:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTATTTTAAAAATTT+AGG - tig0023048:83380-83399 Msa1404210:intergenic 0.0%
!! CAATTTATAGAAAAAAATTA+TGG - tig0023048:82745-82764 Msa1404210:intergenic 10.0%
!!! TTTTCTATAAATTGATTATT+AGG + tig0023048:82751-82770 Msa1404210:intron 10.0%
!!! TTTTGTATTTTAATTAGTTA+GGG - tig0023048:83335-83354 Msa1404210:intergenic 10.0%
!!! TTTTGTATTTTAATTAGTTA+GGG + tig0023048:83335-83354 Msa1404210:intron 10.0%
!!! TTTCTTATGTATTTTTTTAT+TGG + tig0023048:84025-84044 Msa1404210:intron 10.0%
!! TAATAATATTCAATTGCAAA+TGG + tig0023048:82950-82969 Msa1404210:CDS 15.0%
!! AATAATATTCAATTGCAAAT+GGG + tig0023048:82951-82970 Msa1404210:CDS 15.0%
!!! TTTGTATTTTAATTAGTTAG+GGG - tig0023048:83336-83355 Msa1404210:intergenic 15.0%
!!! GTTTTGTATTTTAATTAGTT+AGG - tig0023048:83334-83353 Msa1404210:intergenic 15.0%
!!! GTTTTGTATTTTAATTAGTT+AGG + tig0023048:83334-83353 Msa1404210:intron 15.0%
!!! TTTGTATTTTAATTAGTTAG+GGG + tig0023048:83336-83355 Msa1404210:intron 15.0%
!!! TAATATTAGTTTTGAAAACT+GGG - tig0023048:83578-83597 Msa1404210:intergenic 15.0%
!!! TTAATATTAGTTTTGAAAAC+TGG - tig0023048:83579-83598 Msa1404210:intergenic 15.0%
!!! TTTTTTCAATGATTTAACAT+TGG - tig0023048:83758-83777 Msa1404210:intergenic 15.0%
!! AAAAAAAATTCTTAGTCTAA+AGG + tig0023048:83772-83791 Msa1404210:intron 15.0%
!! AGTATACTATTGTTAAAAAA+AGG - tig0023048:84062-84081 Msa1404210:intergenic 15.0%
!!! TGTTATTTAAACTTAAGATA+AGG + tig0023048:84082-84101 Msa1404210:intron 15.0%
!! TAAAATATATAAAGCTACAA+GGG - tig0023048:84389-84408 Msa1404210:intergenic 15.0%
!! ATAAAATATATAAAGCTACA+AGG - tig0023048:84390-84409 Msa1404210:intergenic 15.0%
!!! TTGAGTTTATTTTTTTTTTG+TGG + tig0023048:84420-84439 Msa1404210:intron 15.0%
!! TTATACAATTAAATAAGGTA+TGG - tig0023048:85216-85235 Msa1404210:intergenic 15.0%
!! ACCTAACATATGATTATATA+GGG - tig0023048:82583-82602 Msa1404210:intergenic 20.0%
!! AACCTAACATATGATTATAT+AGG - tig0023048:82584-82603 Msa1404210:intergenic 20.0%
!! ATATAATCATATGTTAGGTT+AGG + tig0023048:82584-82603 Msa1404210:intron 20.0%
!!! GATTACTTTGTTATTTCATT+TGG + tig0023048:82676-82695 Msa1404210:intron 20.0%
!!! ATGAATGTTTTTGAGTATTA+TGG - tig0023048:82922-82941 Msa1404210:intergenic 20.0%
!!! TGGTAGTTGTATTAAATTTT+TGG + tig0023048:83075-83094 Msa1404210:intron 20.0%
!!! TTGTATTTTAATTAGTTAGG+GGG - tig0023048:83337-83356 Msa1404210:intergenic 20.0%
!!! TTGTATTTTAATTAGTTAGG+GGG + tig0023048:83337-83356 Msa1404210:intron 20.0%
!! AGAATGAGCATAATATTTAA+GGG + tig0023048:83518-83537 Msa1404210:intron 20.0%
!!! TGATTTTCTCTTTTTTCATA+TGG + tig0023048:83676-83695 Msa1404210:intron 20.0%
!! AATACACATTTATACTACAT+GGG + tig0023048:84174-84193 Msa1404210:intron 20.0%
!!! TGAATTGTTCTAGAAATAAT+AGG + tig0023048:84353-84372 Msa1404210:intron 20.0%
!! AAAATATATAAAGCTACAAG+GGG - tig0023048:84388-84407 Msa1404210:intergenic 20.0%
!! TAATGAAATGAGATCTAATT+TGG + tig0023048:84452-84471 Msa1404210:intron 20.0%
!! GTCAATAAACAAAAATTATG+TGG + tig0023048:84618-84637 Msa1404210:CDS 20.0%
!! TAAAAAAGAAATTGTGATGT+CGG + tig0023048:84962-84981 Msa1404210:three_prime_UTR 20.0%
! TCCCTATATAATCATATGTT+AGG + tig0023048:82579-82598 Msa1404210:intron 25.0%
! TACATGAACAACTAATTACA+TGG - tig0023048:82627-82646 Msa1404210:intergenic 25.0%
! GAGAATGAGCATAATATTTA+AGG + tig0023048:83517-83536 Msa1404210:intron 25.0%
! TTAAACTTAAGATAAGGAAG+TGG + tig0023048:84088-84107 Msa1404210:intron 25.0%
! GAATACACATTTATACTACA+TGG + tig0023048:84173-84192 Msa1404210:intron 25.0%
! GCAATTATTAAACACAAAAG+AGG + tig0023048:84231-84250 Msa1404210:intron 25.0%
!! TTCCTTTGGCATAAAATTTT+CGG - tig0023048:84500-84519 Msa1404210:intergenic 25.0%
! AAACAAAAATTATGTGGACA+TGG + tig0023048:84624-84643 Msa1404210:CDS 25.0%
! CAAACTTCAAAGATGTATAT+TGG - tig0023048:84763-84782 Msa1404210:intergenic 25.0%
!! GTATATGTTTTCCAAATTCT+TGG + tig0023048:84904-84923 Msa1404210:three_prime_UTR 25.0%
! AAAAAAGAAATTGTGATGTC+GGG + tig0023048:84963-84982 Msa1404210:three_prime_UTR 25.0%
! ATTTGTGTCACAATAATCAT+TGG - tig0023048:85108-85127 Msa1404210:intergenic 25.0%
! GGATAAAAAAGCATGAGAAA+AGG - tig0023048:82562-82581 Msa1404210:intergenic 30.0%
ACCTAGTTTAACCATTCAAT+CGG + tig0023048:82809-82828 Msa1404210:intron 30.0%
TTTAACCATTCAATCGGTTA+GGG + tig0023048:82815-82834 Msa1404210:intron 30.0%
! ACAAGCATGTGATATTTTGT+TGG + tig0023048:82894-82913 Msa1404210:five_prime_UTR 30.0%
ACTCAAAAACATTCATGTCA+TGG + tig0023048:82926-82945 Msa1404210:exon 30.0%
AATATTCAATTGCAAATGGG+TGG + tig0023048:82954-82973 Msa1404210:CDS 30.0%
ACTATATAGCATACCTCTTA+CGG - tig0023048:83017-83036 Msa1404210:intergenic 30.0%
!!! TTTTGGCTTAATTGCACTTT+TGG + tig0023048:83092-83111 Msa1404210:intron 30.0%
!! TCGCAACTTTTGAAAAGATA+GGG - tig0023048:83120-83139 Msa1404210:intergenic 30.0%
!! ATCGCAACTTTTGAAAAGAT+AGG - tig0023048:83121-83140 Msa1404210:intergenic 30.0%
! CTTTTCAAAAGTTGCGATTT+TGG + tig0023048:83123-83142 Msa1404210:intron 30.0%
! TTTAGGCTTAATTGCACTTT+TGG - tig0023048:83363-83382 Msa1404210:intergenic 30.0%
!!! TTAGTTTTGAAAACTGGGTT+TGG - tig0023048:83573-83592 Msa1404210:intergenic 30.0%
! TATCATTGATGACAAGAACA+TGG - tig0023048:83647-83666 Msa1404210:intergenic 30.0%
TTACGTAGAAGCAACATTTA+TGG + tig0023048:83839-83858 Msa1404210:intron 30.0%
!!! TGCTTCTTTTTTTCTGTTGA+AGG + tig0023048:83862-83881 Msa1404210:intron 30.0%
!!! CTTTTTTTCTGTTGAAGGAA+TGG + tig0023048:83867-83886 Msa1404210:intron 30.0%
TAATTTCACAACTGGTCATT+TGG + tig0023048:83896-83915 Msa1404210:CDS 30.0%
! CATTTGGTGAAAACCATTTA+TGG + tig0023048:83912-83931 Msa1404210:CDS 30.0%
AAATAAGACCGAATGATTGA+TGG - tig0023048:84292-84311 Msa1404210:intergenic 30.0%
! TCCTTTGGCATAAAATTTTC+GGG - tig0023048:84499-84518 Msa1404210:intergenic 30.0%
TTATGGTATACAACTTCCTT+TGG - tig0023048:84514-84533 Msa1404210:intergenic 30.0%
ACAGCTTATCATGTATGTTA+TGG - tig0023048:84531-84550 Msa1404210:intergenic 30.0%
! TATTCCTTATTTGCTTCGTT+TGG - tig0023048:84677-84696 Msa1404210:intergenic 30.0%
CAAATGTGCTTCATATTATG+CGG + tig0023048:84810-84829 Msa1404210:three_prime_UTR 30.0%
GGAATTAGAGATAATGACAA+TGG + tig0023048:84939-84958 Msa1404210:three_prime_UTR 30.0%
! AACCATGATTTTCGAAGGAT+TGG + tig0023048:82491-82510 Msa1404210:five_prime_UTR 35.0%
TTCCAATCCTTCGAAAATCA+TGG - tig0023048:82496-82515 Msa1404210:intergenic 35.0%
! ATCATCGATTGATGATACCT+TGG - tig0023048:82539-82558 Msa1404210:intergenic 35.0%
AGGTCAAGACAACAAAGTTA+GGG - tig0023048:82793-82812 Msa1404210:intergenic 35.0%
TAGGTCAAGACAACAAAGTT+AGG - tig0023048:82794-82813 Msa1404210:intergenic 35.0%
ACCGATTGAATGGTTAAACT+AGG - tig0023048:82813-82832 Msa1404210:intergenic 35.0%
GTTTAACCATTCAATCGGTT+AGG + tig0023048:82814-82833 Msa1404210:intron 35.0%
!!! TTAGGGTTTTGACTTCTTGT+GGG + tig0023048:82832-82851 Msa1404210:intron 35.0%
CATATTGCTGGTGATAACAA+AGG + tig0023048:82868-82887 Msa1404210:five_prime_UTR 35.0%
! TACGGTATTTAGGATCAACA+AGG - tig0023048:82999-83018 Msa1404210:intergenic 35.0%
TTGATCCTAAATACCGTAAG+AGG + tig0023048:83001-83020 Msa1404210:CDS 35.0%
!! GCAACTTTTGAAAAGATAGG+GGG - tig0023048:83118-83137 Msa1404210:intergenic 35.0%
!! CGCAACTTTTGAAAAGATAG+GGG - tig0023048:83119-83138 Msa1404210:intergenic 35.0%
TGCCAAAGATCCAATACATA+GGG - tig0023048:83307-83326 Msa1404210:intergenic 35.0%
! TATTGGATCTTTGGCAGTTT+TGG + tig0023048:83183-83202 Msa1404210:intron 35.0%
! TATTGGATCTTTGGCAGTTT+TGG - tig0023048:83183-83202 Msa1404210:intergenic 35.0%
TGCCAAAGATCCAATACATA+AGG + tig0023048:83307-83326 Msa1404210:intron 35.0%
GCCAAAGATCCAATACATAA+GGG + tig0023048:83308-83327 Msa1404210:intron 35.0%
CCAAAGATCCAATACATAAG+GGG + tig0023048:83309-83328 Msa1404210:intron 35.0%
! ACAGTTTGAGATTTGTCCTA+CGG + tig0023048:83414-83433 Msa1404210:intron 35.0%
!!! GACATGGGTTTTTTACAGTA+TGG - tig0023048:83494-83513 Msa1404210:intergenic 35.0%
CTACGTAAAATTGCATAGCT+TGG - tig0023048:83827-83846 Msa1404210:intergenic 35.0%
!!! GGTGCTTTTAATTTCACAAC+TGG + tig0023048:83888-83907 Msa1404210:CDS 35.0%
! CAGTAAAAGCACACCATAAA+TGG - tig0023048:83928-83947 Msa1404210:intergenic 35.0%
! ACTACATGGGTGCTATAAAT+TGG + tig0023048:84187-84206 Msa1404210:intron 35.0%
! CTACATGGGTGCTATAAATT+GGG + tig0023048:84188-84207 Msa1404210:intron 35.0%
! TACATGGGTGCTATAAATTG+GGG + tig0023048:84189-84208 Msa1404210:intron 35.0%
!! ATCTAATTTGGTGTTGATGC+AGG + tig0023048:84464-84483 Msa1404210:intron 35.0%
CATACATGATAAGCTGTTGT+TGG + tig0023048:84534-84553 Msa1404210:CDS 35.0%
CGGAAATCATGTTCAACTTT+GGG - tig0023048:84567-84586 Msa1404210:intergenic 35.0%
ACGGAAATCATGTTCAACTT+TGG - tig0023048:84568-84587 Msa1404210:intergenic 35.0%
AAAGTTGAACATGATTTCCG+TGG + tig0023048:84567-84586 Msa1404210:CDS 35.0%
AGAACCAAACGAAGCAAATA+AGG + tig0023048:84670-84689 Msa1404210:CDS 35.0%
AAGCAAATAAGGAATAGCGT+TGG + tig0023048:84681-84700 Msa1404210:exon 35.0%
GTGGAGACAAATCATAAATC+CGG + tig0023048:84832-84851 Msa1404210:three_prime_UTR 35.0%
TTCTAGCATATGTAGGTATC+CGG - tig0023048:84854-84873 Msa1404210:intergenic 35.0%
ATATACTGCAATGTGGTATG+GGG - tig0023048:84890-84909 Msa1404210:intergenic 35.0%
CATATACTGCAATGTGGTAT+GGG - tig0023048:84891-84910 Msa1404210:intergenic 35.0%
ACATATACTGCAATGTGGTA+TGG - tig0023048:84892-84911 Msa1404210:intergenic 35.0%
GGAAAACATATACTGCAATG+TGG - tig0023048:84897-84916 Msa1404210:intergenic 35.0%
! CTCTTGTTTGTCCAAGAATT+TGG - tig0023048:84918-84937 Msa1404210:intergenic 35.0%
AATTCTTGGACAAACAAGAG+AGG + tig0023048:84918-84937 Msa1404210:three_prime_UTR 35.0%
!!! GTCTTTTTCTGGTTTCTATC+CGG - tig0023048:85040-85059 Msa1404210:intergenic 35.0%
! ACTCTGTTGTTGTCTTTTTC+TGG - tig0023048:85051-85070 Msa1404210:intergenic 35.0%
! GGCAAAACCATGATTTTCGA+AGG + tig0023048:82486-82505 Msa1404210:five_prime_UTR 40.0%
ATGATACCTTGGTTTCCGAA+TGG - tig0023048:82528-82547 Msa1404210:intergenic 40.0%
CAAAACCCTAACCGATTGAA+TGG - tig0023048:82823-82842 Msa1404210:intergenic 40.0%
!!! GTTAGGGTTTTGACTTCTTG+TGG + tig0023048:82831-82850 Msa1404210:intron 40.0%
TAGGATCAACAAGGTTGTGA+AGG - tig0023048:82990-83009 Msa1404210:intergenic 40.0%
GCATACCTCTTACGGTATTT+AGG - tig0023048:83009-83028 Msa1404210:intergenic 40.0%
CCAAAGATCCAATACATAGG+GGG - tig0023048:83177-83196 Msa1404210:intergenic 40.0%
GCCAAAGATCCAATACATAG+GGG - tig0023048:83178-83197 Msa1404210:intergenic 40.0%
CTGCCAAAGATCCAATACAT+AGG - tig0023048:83306-83325 Msa1404210:intergenic 40.0%
! TTAAGTCAAAAGTGGCCTTG+GGG - tig0023048:83210-83229 Msa1404210:intergenic 40.0%
CTTAAGTCAAAAGTGGCCTT+GGG - tig0023048:83211-83230 Msa1404210:intergenic 40.0%
ACTTAAGTCAAAAGTGGCCT+TGG - tig0023048:83212-83231 Msa1404210:intergenic 40.0%
CACCTCACTTAAGTCAAAAG+TGG - tig0023048:83218-83237 Msa1404210:intergenic 40.0%
AGCGAAGACTAAGTCAAAAG+TGG + tig0023048:83268-83287 Msa1404210:intron 40.0%
ACTAAGTCAAAAGTGGCCTT+GGG + tig0023048:83275-83294 Msa1404210:intron 40.0%
CCCCTTATGTATTGGATCTT+TGG - tig0023048:83312-83331 Msa1404210:intergenic 40.0%
CAAAACAGCCCCTTATGTAT+TGG - tig0023048:83320-83339 Msa1404210:intergenic 40.0%
CAACAACTGATATTGACCGT+AGG - tig0023048:83433-83452 Msa1404210:intergenic 40.0%
ATGCTCATTCTCGATGACAT+GGG - tig0023048:83509-83528 Msa1404210:intergenic 40.0%
TATGCTCATTCTCGATGACA+TGG - tig0023048:83510-83529 Msa1404210:intergenic 40.0%
TAATTATGCCACCCACATGA+GGG - tig0023048:83974-83993 Msa1404210:intergenic 40.0%
ATAATTATGCCACCCACATG+AGG - tig0023048:83975-83994 Msa1404210:intergenic 40.0%
TAGCGCACTTTCTCTTCATT+TGG - tig0023048:84127-84146 Msa1404210:intergenic 40.0%
! GCCCGAAAATTTTATGCCAA+AGG + tig0023048:84495-84514 Msa1404210:CDS 40.0%
TTTATTGCACTCTTCGTCCA+CGG - tig0023048:84587-84606 Msa1404210:intergenic 40.0%
! CAAATAAGGAATAGCGTTGG+TGG + tig0023048:84684-84703 Msa1404210:exon 40.0%
ATGTGCTTCATATTATGCGG+TGG + tig0023048:84813-84832 Msa1404210:three_prime_UTR 40.0%
CAGCTTCTTCTAGCATATGT+AGG - tig0023048:84861-84880 Msa1404210:intergenic 40.0%
CTACATATGCTAGAAGAAGC+TGG + tig0023048:84859-84878 Msa1404210:three_prime_UTR 40.0%
!!! GGTTTTGACTTCTTGTGGGA+TGG + tig0023048:82836-82855 Msa1404210:five_prime_UTR 45.0%
! TTTGACTTCTTGTGGGATGG+AGG + tig0023048:82839-82858 Msa1404210:five_prime_UTR 45.0%
TTGACTTCTTGTGGGATGGA+GGG + tig0023048:82840-82859 Msa1404210:five_prime_UTR 45.0%
!! TGGAGGGAGTATCATATTGC+TGG + tig0023048:82856-82875 Msa1404210:five_prime_UTR 45.0%
GAATAGCACACGATACATGC+TGG + tig0023048:83055-83074 Msa1404210:intron 45.0%
CAAAACAGCCCCCTATGTAT+TGG + tig0023048:83166-83185 Msa1404210:intron 45.0%
CCCCCTATGTATTGGATCTT+TGG + tig0023048:83174-83193 Msa1404210:intron 45.0%
! TAAGTCAAAAGTGGCCTTGG+GGG - tig0023048:83277-83296 Msa1404210:intergenic 45.0%
!!! GGCCACTTTTGACTTAAGTG+AGG + tig0023048:83213-83232 Msa1404210:intron 45.0%
GACTAAGTCAAAAGTGGCCT+TGG + tig0023048:83274-83293 Msa1404210:intron 45.0%
! CTAAGTCAAAAGTGGCCTTG+GGG + tig0023048:83276-83295 Msa1404210:intron 45.0%
! TAAGTCAAAAGTGGCCTTGG+GGG + tig0023048:83277-83296 Msa1404210:intron 45.0%
TTCATATGGTTCATGCGCAG+TGG + tig0023048:83690-83709 Msa1404210:intron 45.0%
AGCTTGGTACACGAAAACGA+TGG - tig0023048:83811-83830 Msa1404210:intergenic 45.0%
GCATGTGGCCATCAATCATT+CGG + tig0023048:84281-84300 Msa1404210:intron 45.0%
!! AGGAATAGCGTTGGTGGAAA+AGG + tig0023048:84690-84709 Msa1404210:exon 45.0%
TTGGAACAACCAGCGCCATT+CGG + tig0023048:82510-82529 Msa1404210:five_prime_UTR 50.0%
TGCCACATGTCGACCATTGT+GGG + tig0023048:83236-83255 Msa1404210:intron 50.0%
! TGACTTAGTCTTCGCTGACG+TGG - tig0023048:83264-83283 Msa1404210:intergenic 50.0%
CATGCATGCAGTAGCACTAG+TGG + tig0023048:83725-83744 Msa1404210:intron 50.0%
ACACAAGAGTGAGCTGCATG+TGG + tig0023048:84266-84285 Msa1404210:intron 50.0%
! GCGTTGGTGGAAAAGGTGAA+AGG + tig0023048:84697-84716 Msa1404210:intron 50.0%
ACTCGATTCCTACCAGCACA+AGG + tig0023048:85009-85028 Msa1404210:three_prime_UTR 50.0%
!! CTACCAGCACAAGGAGTATC+CGG + tig0023048:85018-85037 Msa1404210:three_prime_UTR 50.0%
TATCCGGATACTCCTTGTGC+TGG - tig0023048:85024-85043 Msa1404210:intergenic 50.0%
!! TTTGGCAGTTTTGGCCCCCA+AGG + tig0023048:83192-83211 Msa1404210:intron 55.0%
GTGCCACATGTCGACCATTG+TGG + tig0023048:83235-83254 Msa1404210:intron 55.0%
CACCCACAATGGTCGACATG+TGG - tig0023048:83241-83260 Msa1404210:intergenic 55.0%
!! TTTGGCAGTTTTGGCCCCCA+AGG - tig0023048:83192-83211 Msa1404210:intergenic 55.0%
CATGCAGTAGCACTAGTGGC+TGG + tig0023048:83729-83748 Msa1404210:intron 55.0%
! TGATGATGCCAGCCCTCATG+TGG + tig0023048:83959-83978 Msa1404210:intron 55.0%
GATGATGCCAGCCCTCATGT+GGG + tig0023048:83960-83979 Msa1404210:intron 55.0%
! CGGATACTCCTTGTGCTGGT+AGG - tig0023048:85020-85039 Msa1404210:intergenic 55.0%
CCTTGGTTTCCGAATGGCGC+TGG - tig0023048:82522-82541 Msa1404210:intergenic 60.0%
CCAGCGCCATTCGGAAACCA+AGG + tig0023048:82519-82538 Msa1404210:five_prime_UTR 60.0%
TATGCCACCCACATGAGGGC+TGG - tig0023048:83970-83989 Msa1404210:intergenic 60.0%
CGCTGACGTGGCACCCACAA+TGG - tig0023048:83252-83271 Msa1404210:intergenic 65.0%
GATGCCAGCCCTCATGTGGG+TGG + tig0023048:83963-83982 Msa1404210:intron 65.0%
Chromosome Type Strat End Strand Name
tig0023048 gene 82479 85238 82479 ID=Msa1404210;Name=Msa1404210
tig0023048 mRNA 82479 85238 82479 ID=Msa1404210-mRNA-1;Parent=Msa1404210;Name=Msa1404210-mRNA-1;_AED=0.24;_eAED=0.24;_QI=166|0.5|0.8|0.8|0.5|0.2|5|460|116
tig0023048 exon 82479 82540 82479 ID=Msa1404210-mRNA-1:exon:19672;Parent=Msa1404210-mRNA-1
tig0023048 exon 82836 83022 82836 ID=Msa1404210-mRNA-1:exon:19673;Parent=Msa1404210-mRNA-1
tig0023048 exon 83884 83946 83884 ID=Msa1404210-mRNA-1:exon:19674;Parent=Msa1404210-mRNA-1
tig0023048 exon 84486 84711 84486 ID=Msa1404210-mRNA-1:exon:19675;Parent=Msa1404210-mRNA-1
tig0023048 exon 84800 85238 84800 ID=Msa1404210-mRNA-1:exon:19676;Parent=Msa1404210-mRNA-1
tig0023048 five_prime_UTR 82479 82540 82479 ID=Msa1404210-mRNA-1:five_prime_utr;Parent=Msa1404210-mRNA-1
tig0023048 five_prime_UTR 82836 82939 82836 ID=Msa1404210-mRNA-1:five_prime_utr;Parent=Msa1404210-mRNA-1
tig0023048 CDS 82940 83022 82940 ID=Msa1404210-mRNA-1:cds;Parent=Msa1404210-mRNA-1
tig0023048 CDS 83884 83946 83884 ID=Msa1404210-mRNA-1:cds;Parent=Msa1404210-mRNA-1
tig0023048 CDS 84486 84690 84486 ID=Msa1404210-mRNA-1:cds;Parent=Msa1404210-mRNA-1
tig0023048 three_prime_UTR 84691 84711 84691 ID=Msa1404210-mRNA-1:three_prime_utr;Parent=Msa1404210-mRNA-1
tig0023048 three_prime_UTR 84800 85238 84800 ID=Msa1404210-mRNA-1:three_prime_utr;Parent=Msa1404210-mRNA-1
Gene Sequence

>Msa1404210

ATGTCATGGATAATAATATTCAATTGCAAATGGGTGGCAAAAGCAAACCTTCACAACCTTGTTGATCCTAAATACCGTAAGAGGAATGGTGCTTTTAATTTCACAACTGGTCATTTGGTGAAAACCATTTATGGTGTGCTTTTACTGTTCAACATGCCCGAAAATTTTATGCCAAAGGAAGTTGTATACCATAACATACATGATAAGCTGTTGTTGGATTCTATCCCAAAGTTGAACATGATTTCCGTGGACGAAGAGTGCAATAAACTTCTCATAGAGTCAATAAACAAAAATTATGTGGACATGGCTGATTATTTGATGAGTATCAACAGAACCAAACGAAGCAAATAA
Protein sequence

>Msa1404210

MSWIIIFNCKWVAKANLHNLVDPKYRKRNGAFNFTTGHLVKTIYGVLLLFNMPENFMPKEVVYHNIHDKLLLDSIPKLNMISVDEECNKLLIESINKNYVDMADYLMSINRTKRSK*