AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0625080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625080 MtrunA17_Chr4g0000411 99.259 135 1 0 2 136 344 478 1.46e-95 283
Msa0625080 MtrunA17_Chr4g0002141 83.088 136 23 0 1 136 301 436 6.12e-82 247
Msa0625080 MtrunA17_Chr4g0051481 99.057 106 1 0 3 108 19 124 4.77e-75 219
Msa0625080 MtrunA17_Chr2g0289421 61.481 135 52 0 2 136 294 428 6.39e-61 192
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625080 AT1G78880.1 79.259 135 28 0 2 136 334 468 1.29e-73 227
Msa0625080 AT1G16860.1 77.037 135 31 0 2 136 340 474 1.26e-70 219
Msa0625080 AT4G22290.1 61.481 135 52 0 2 136 311 445 5.43e-60 191

Find 54 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCGATTTCCTTCGATGGTT+GGG 0.253082 4_3:+4082974 Msa0625080:CDS
TGTTTCGATCTGTTGATTAA+TGG 0.256726 4_3:+4083637 Msa0625080:three_prime_UTR
TTCTTTGATAAAACAATTGA+TGG 0.320664 4_3:+4083372 Msa0625080:three_prime_UTR
AGGAAATTCACGGATGTAAA+TGG 0.325990 4_3:-4083471 None:intergenic
TACATCTCTGACTTCCAATC+TGG 0.350537 4_3:+4082851 Msa0625080:CDS
CCCCGATTTCCTTCGATGGT+TGG 0.361108 4_3:+4082973 Msa0625080:CDS
CCCCAACCATCGAAGGAAAT+CGG 0.367647 4_3:-4082975 None:intergenic
CTGAGAGCATTGGTTAAAAC+AGG 0.383057 4_3:+4082875 Msa0625080:CDS
CCCAACCATCGAAGGAAATC+GGG 0.393298 4_3:-4082974 None:intergenic
GCATTGGTTAAAACAGGCTT+TGG 0.395936 4_3:+4082881 Msa0625080:CDS
GGTAGCACGGTTAGCGTAAT+GGG 0.397403 4_3:+4083146 Msa0625080:CDS
ATCTCAAAACAATACCTTCA+AGG 0.409889 4_3:-4083265 None:intergenic
TGTAAGCATATATGTGTTGT+TGG 0.422451 4_3:+4083427 Msa0625080:three_prime_UTR
CCAATGCTCTCAGGCCAGAT+TGG 0.427585 4_3:-4082865 None:intergenic
ACAATACCTTCAAGGCTAGC+TGG 0.445053 4_3:-4083257 None:intergenic
TGATGGTGGTGGTGGGTGGG+TGG 0.447382 4_3:+4083389 Msa0625080:three_prime_UTR
CCGATTTCCTTCGATGGTTG+GGG 0.450657 4_3:+4082975 Msa0625080:CDS
CGTATCATGCAGTTAAAAGA+AGG 0.462235 4_3:+4083022 Msa0625080:CDS
CCTCCTGAGCCTTTGACGAC+CGG 0.462807 4_3:+4083209 Msa0625080:CDS
CATCCGGTCGTCAAAGGCTC+AGG 0.463709 4_3:-4083212 None:intergenic
GGGGACATGTCTTTATTCTC+CGG 0.465892 4_3:-4082953 None:intergenic
CAATTGATGGTGGTGGTGGG+TGG 0.479323 4_3:+4083385 Msa0625080:three_prime_UTR
TGAGTTGAGTATGAAACAAA+AGG 0.481992 4_3:-4083491 None:intergenic
CTGAGTGAGTGAGTCTCTCT+AGG 0.482847 4_3:+4083341 Msa0625080:three_prime_UTR
CGTTGTAATTGATGTCAATC+CGG 0.482923 4_3:+4082934 Msa0625080:CDS
AGGTAGCACGGTTAGCGTAA+TGG 0.490906 4_3:+4083145 Msa0625080:CDS
CACTGGCATCCGGTCGTCAA+AGG 0.491248 4_3:-4083218 None:intergenic
ACATTTCGCCCACTGGCATC+CGG 0.492145 4_3:-4083228 None:intergenic
AAAACAATTGATGGTGGTGG+TGG 0.496287 4_3:+4083381 Msa0625080:three_prime_UTR
ATTAGCTATACAACAAACTA+AGG 0.496458 4_3:-4083515 None:intergenic
AATTGATGGTGGTGGTGGGT+GGG 0.496921 4_3:+4083386 Msa0625080:three_prime_UTR
GTCGTCAAAGGCTCAGGAGG+AGG 0.497151 4_3:-4083206 None:intergenic
ACAACGGAATCGTCAACATA+AGG 0.503635 4_3:-4082917 None:intergenic
CCAATCTGGCCTGAGAGCAT+TGG 0.509660 4_3:+4082865 Msa0625080:CDS
TAAAACAGGCTTTGGTGCAA+GGG 0.531505 4_3:+4082889 Msa0625080:CDS
TTAAAACAGGCTTTGGTGCA+AGG 0.533222 4_3:+4082888 Msa0625080:CDS
ATGAAACAAAAGGAAATTCA+CGG 0.533327 4_3:-4083481 None:intergenic
TTCTCTTCCCCAACCATCGA+AGG 0.543040 4_3:-4082982 None:intergenic
TGATACGATCATCACTAGAA+AGG 0.559142 4_3:-4083006 None:intergenic
CTAGAGAGACTCACTCACTC+AGG 0.567812 4_3:-4083340 None:intergenic
CCGGTCGTCAAAGGCTCAGG+AGG 0.581282 4_3:-4083209 None:intergenic
AAACAATTGATGGTGGTGGT+GGG 0.585983 4_3:+4083382 Msa0625080:three_prime_UTR
GATAAAACAATTGATGGTGG+TGG 0.587903 4_3:+4083378 Msa0625080:three_prime_UTR
TAGAGAGACTCACTCACTCA+GGG 0.589043 4_3:-4083339 None:intergenic
CTTTGACGACCGGATGCCAG+TGG 0.590601 4_3:+4083219 Msa0625080:CDS
TAATTCAATGCAGAGACATG+TGG 0.616761 4_3:+4082820 None:intergenic
GTATCATGCAGTTAAAAGAA+GGG 0.623123 4_3:+4083023 Msa0625080:CDS
TTTGATAAAACAATTGATGG+TGG 0.633000 4_3:+4083375 Msa0625080:three_prime_UTR
CCAACCATCGAAGGAAATCG+GGG 0.644123 4_3:-4082973 None:intergenic
CGGATTGACATCAATTACAA+CGG 0.656746 4_3:-4082933 None:intergenic
GTACATAAAAGAAGGTAGCA+CGG 0.660164 4_3:+4083133 Msa0625080:CDS
TTTGACGACCGGATGCCAGT+GGG 0.664024 4_3:+4083220 Msa0625080:CDS
CAACCATCGAAGGAAATCGG+GGG 0.713932 4_3:-4082972 None:intergenic
AGAGAGACTCACTCACTCAG+GGG 0.726067 4_3:-4083338 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTTTGATAAAACAATTGA+TGG + chr4_3:4083372-4083391 Msa0625080:three_prime_UTR 20.0%
!!! AGAATTTCAAACTTTTTGAA+TGG + chr4_3:4083567-4083586 Msa0625080:three_prime_UTR 20.0%
!! TTCTTTGATAAAACAATTGA+TGG + chr4_3:4083372-4083391 Msa0625080:three_prime_UTR 20.0%
!!! AGAATTTCAAACTTTTTGAA+TGG + chr4_3:4083567-4083586 Msa0625080:three_prime_UTR 20.0%
! AAATGAAGTTCTTATGAAAG+TGG + chr4_3:4083090-4083109 Msa0625080:intron 25.0%
!! TTTTGTAGGTACATAAAAGA+AGG + chr4_3:4083125-4083144 Msa0625080:intron 25.0%
! TTTGATAAAACAATTGATGG+TGG + chr4_3:4083375-4083394 Msa0625080:three_prime_UTR 25.0%
! ATGAAACAAAAGGAAATTCA+CGG - chr4_3:4083484-4083503 None:intergenic 25.0%
! ATTAGCTATACAACAAACTA+AGG - chr4_3:4083518-4083537 None:intergenic 25.0%
!! CAAAAAGTTTGAAATTCTCA+AGG - chr4_3:4083565-4083584 None:intergenic 25.0%
! AAATGAAGTTCTTATGAAAG+TGG + chr4_3:4083090-4083109 Msa0625080:intron 25.0%
!! TTTTGTAGGTACATAAAAGA+AGG + chr4_3:4083125-4083144 Msa0625080:intron 25.0%
! TTTGATAAAACAATTGATGG+TGG + chr4_3:4083375-4083394 Msa0625080:three_prime_UTR 25.0%
! ATGAAACAAAAGGAAATTCA+CGG - chr4_3:4083484-4083503 None:intergenic 25.0%
! ATTAGCTATACAACAAACTA+AGG - chr4_3:4083518-4083537 None:intergenic 25.0%
!! CAAAAAGTTTGAAATTCTCA+AGG - chr4_3:4083565-4083584 None:intergenic 25.0%
GTATCATGCAGTTAAAAGAA+GGG + chr4_3:4083023-4083042 Msa0625080:CDS 30.0%
ATCTCAAAACAATACCTTCA+AGG - chr4_3:4083268-4083287 None:intergenic 30.0%
GGATAAAAAGATAACTACAC+TGG - chr4_3:4083320-4083339 None:intergenic 30.0%
TGTAAGCATATATGTGTTGT+TGG + chr4_3:4083427-4083446 Msa0625080:three_prime_UTR 30.0%
! TGAGTTGAGTATGAAACAAA+AGG - chr4_3:4083494-4083513 None:intergenic 30.0%
! TGTTTCGATCTGTTGATTAA+TGG + chr4_3:4083637-4083656 Msa0625080:three_prime_UTR 30.0%
GTATCATGCAGTTAAAAGAA+GGG + chr4_3:4083023-4083042 Msa0625080:CDS 30.0%
ATCTCAAAACAATACCTTCA+AGG - chr4_3:4083268-4083287 None:intergenic 30.0%
GGATAAAAAGATAACTACAC+TGG - chr4_3:4083320-4083339 None:intergenic 30.0%
TGTAAGCATATATGTGTTGT+TGG + chr4_3:4083427-4083446 Msa0625080:three_prime_UTR 30.0%
! TGAGTTGAGTATGAAACAAA+AGG - chr4_3:4083494-4083513 None:intergenic 30.0%
! TGTTTCGATCTGTTGATTAA+TGG + chr4_3:4083637-4083656 Msa0625080:three_prime_UTR 30.0%
CGGATTGACATCAATTACAA+CGG - chr4_3:4082936-4082955 None:intergenic 35.0%
CGTTGTAATTGATGTCAATC+CGG + chr4_3:4082934-4082953 Msa0625080:CDS 35.0%
TGATACGATCATCACTAGAA+AGG - chr4_3:4083009-4083028 None:intergenic 35.0%
CGTATCATGCAGTTAAAAGA+AGG + chr4_3:4083022-4083041 Msa0625080:CDS 35.0%
TGAAGTTCTTATGAAAGTGG+CGG + chr4_3:4083093-4083112 Msa0625080:intron 35.0%
GTACATAAAAGAAGGTAGCA+CGG + chr4_3:4083133-4083152 Msa0625080:CDS 35.0%
!! GATAAAACAATTGATGGTGG+TGG + chr4_3:4083378-4083397 Msa0625080:three_prime_UTR 35.0%
AGGAAATTCACGGATGTAAA+TGG - chr4_3:4083474-4083493 None:intergenic 35.0%
CGGATTGACATCAATTACAA+CGG - chr4_3:4082936-4082955 None:intergenic 35.0%
CGTTGTAATTGATGTCAATC+CGG + chr4_3:4082934-4082953 Msa0625080:CDS 35.0%
TGATACGATCATCACTAGAA+AGG - chr4_3:4083009-4083028 None:intergenic 35.0%
CGTATCATGCAGTTAAAAGA+AGG + chr4_3:4083022-4083041 Msa0625080:CDS 35.0%
TGAAGTTCTTATGAAAGTGG+CGG + chr4_3:4083093-4083112 Msa0625080:intron 35.0%
GTACATAAAAGAAGGTAGCA+CGG + chr4_3:4083133-4083152 Msa0625080:CDS 35.0%
!! GATAAAACAATTGATGGTGG+TGG + chr4_3:4083378-4083397 Msa0625080:three_prime_UTR 35.0%
AGGAAATTCACGGATGTAAA+TGG - chr4_3:4083474-4083493 None:intergenic 35.0%
TACATCTCTGACTTCCAATC+TGG + chr4_3:4082851-4082870 Msa0625080:CDS 40.0%
!!! CTGTTTTAACCAATGCTCTC+AGG - chr4_3:4082877-4082896 None:intergenic 40.0%
!! CTGAGAGCATTGGTTAAAAC+AGG + chr4_3:4082875-4082894 Msa0625080:CDS 40.0%
!! GCATTGGTTAAAACAGGCTT+TGG + chr4_3:4082881-4082900 Msa0625080:CDS 40.0%
! TTAAAACAGGCTTTGGTGCA+AGG + chr4_3:4082888-4082907 Msa0625080:CDS 40.0%
! TAAAACAGGCTTTGGTGCAA+GGG + chr4_3:4082889-4082908 Msa0625080:CDS 40.0%
ACAACGGAATCGTCAACATA+AGG - chr4_3:4082920-4082939 None:intergenic 40.0%
GAAAAATACATTTCGCCCAC+TGG - chr4_3:4083238-4083257 None:intergenic 40.0%
!!! ATTTTTCCAGCTAGCCTTGA+AGG + chr4_3:4083251-4083270 Msa0625080:CDS 40.0%
!! AAAACAATTGATGGTGGTGG+TGG + chr4_3:4083381-4083400 Msa0625080:three_prime_UTR 40.0%
!! AAACAATTGATGGTGGTGGT+GGG + chr4_3:4083382-4083401 Msa0625080:three_prime_UTR 40.0%
TACATCTCTGACTTCCAATC+TGG + chr4_3:4082851-4082870 Msa0625080:CDS 40.0%
!!! CTGTTTTAACCAATGCTCTC+AGG - chr4_3:4082877-4082896 None:intergenic 40.0%
!! CTGAGAGCATTGGTTAAAAC+AGG + chr4_3:4082875-4082894 Msa0625080:CDS 40.0%
!! GCATTGGTTAAAACAGGCTT+TGG + chr4_3:4082881-4082900 Msa0625080:CDS 40.0%
! TTAAAACAGGCTTTGGTGCA+AGG + chr4_3:4082888-4082907 Msa0625080:CDS 40.0%
! TAAAACAGGCTTTGGTGCAA+GGG + chr4_3:4082889-4082908 Msa0625080:CDS 40.0%
ACAACGGAATCGTCAACATA+AGG - chr4_3:4082920-4082939 None:intergenic 40.0%
GAAAAATACATTTCGCCCAC+TGG - chr4_3:4083238-4083257 None:intergenic 40.0%
!!! ATTTTTCCAGCTAGCCTTGA+AGG + chr4_3:4083251-4083270 Msa0625080:CDS 40.0%
!! AAAACAATTGATGGTGGTGG+TGG + chr4_3:4083381-4083400 Msa0625080:three_prime_UTR 40.0%
!! AAACAATTGATGGTGGTGGT+GGG + chr4_3:4083382-4083401 Msa0625080:three_prime_UTR 40.0%
GGGGACATGTCTTTATTCTC+CGG - chr4_3:4082956-4082975 None:intergenic 45.0%
!! GGCGGCTATGATGTTTTTGT+AGG + chr4_3:4083111-4083130 Msa0625080:intron 45.0%
ACAATACCTTCAAGGCTAGC+TGG - chr4_3:4083260-4083279 None:intergenic 45.0%
TAGAGAGACTCACTCACTCA+GGG - chr4_3:4083342-4083361 None:intergenic 45.0%
TCAACCACATGTCTCTGCAT+TGG - chr4_3:4082827-4082846 None:intergenic 45.0%
GGGGACATGTCTTTATTCTC+CGG - chr4_3:4082956-4082975 None:intergenic 45.0%
!! GGCGGCTATGATGTTTTTGT+AGG + chr4_3:4083111-4083130 Msa0625080:intron 45.0%
ACAATACCTTCAAGGCTAGC+TGG - chr4_3:4083260-4083279 None:intergenic 45.0%
TAGAGAGACTCACTCACTCA+GGG - chr4_3:4083342-4083361 None:intergenic 45.0%
CAACCATCGAAGGAAATCGG+GGG - chr4_3:4082975-4082994 None:intergenic 50.0%
CCAACCATCGAAGGAAATCG+GGG - chr4_3:4082976-4082995 None:intergenic 50.0%
CCCAACCATCGAAGGAAATC+GGG - chr4_3:4082977-4082996 None:intergenic 50.0%
CCCGATTTCCTTCGATGGTT+GGG + chr4_3:4082974-4082993 Msa0625080:CDS 50.0%
CCCCAACCATCGAAGGAAAT+CGG - chr4_3:4082978-4082997 None:intergenic 50.0%
CCGATTTCCTTCGATGGTTG+GGG + chr4_3:4082975-4082994 Msa0625080:CDS 50.0%
TTCTCTTCCCCAACCATCGA+AGG - chr4_3:4082985-4083004 None:intergenic 50.0%
AGGTAGCACGGTTAGCGTAA+TGG + chr4_3:4083145-4083164 Msa0625080:CDS 50.0%
GGTAGCACGGTTAGCGTAAT+GGG + chr4_3:4083146-4083165 Msa0625080:CDS 50.0%
AGAGAGACTCACTCACTCAG+GGG - chr4_3:4083341-4083360 None:intergenic 50.0%
CTAGAGAGACTCACTCACTC+AGG - chr4_3:4083343-4083362 None:intergenic 50.0%
CTGAGTGAGTGAGTCTCTCT+AGG + chr4_3:4083341-4083360 Msa0625080:three_prime_UTR 50.0%
!! AATTGATGGTGGTGGTGGGT+GGG + chr4_3:4083386-4083405 Msa0625080:three_prime_UTR 50.0%
CAACCATCGAAGGAAATCGG+GGG - chr4_3:4082975-4082994 None:intergenic 50.0%
CCAACCATCGAAGGAAATCG+GGG - chr4_3:4082976-4082995 None:intergenic 50.0%
CCCAACCATCGAAGGAAATC+GGG - chr4_3:4082977-4082996 None:intergenic 50.0%
CCCGATTTCCTTCGATGGTT+GGG + chr4_3:4082974-4082993 Msa0625080:CDS 50.0%
CCCCAACCATCGAAGGAAAT+CGG - chr4_3:4082978-4082997 None:intergenic 50.0%
CCGATTTCCTTCGATGGTTG+GGG + chr4_3:4082975-4082994 Msa0625080:CDS 50.0%
TTCTCTTCCCCAACCATCGA+AGG - chr4_3:4082985-4083004 None:intergenic 50.0%
AGGTAGCACGGTTAGCGTAA+TGG + chr4_3:4083145-4083164 Msa0625080:CDS 50.0%
GGTAGCACGGTTAGCGTAAT+GGG + chr4_3:4083146-4083165 Msa0625080:CDS 50.0%
AGAGAGACTCACTCACTCAG+GGG - chr4_3:4083341-4083360 None:intergenic 50.0%
CTAGAGAGACTCACTCACTC+AGG - chr4_3:4083343-4083362 None:intergenic 50.0%
CTGAGTGAGTGAGTCTCTCT+AGG + chr4_3:4083341-4083360 Msa0625080:three_prime_UTR 50.0%
!! AATTGATGGTGGTGGTGGGT+GGG + chr4_3:4083386-4083405 Msa0625080:three_prime_UTR 50.0%
CCAATGCTCTCAGGCCAGAT+TGG - chr4_3:4082868-4082887 None:intergenic 55.0%
! CCAATCTGGCCTGAGAGCAT+TGG + chr4_3:4082865-4082884 Msa0625080:CDS 55.0%
TGTCCCCCGATTTCCTTCGA+TGG + chr4_3:4082969-4082988 Msa0625080:CDS 55.0%
CCCCGATTTCCTTCGATGGT+TGG + chr4_3:4082973-4082992 Msa0625080:CDS 55.0%
TTTGACGACCGGATGCCAGT+GGG + chr4_3:4083220-4083239 Msa0625080:CDS 55.0%
ACATTTCGCCCACTGGCATC+CGG - chr4_3:4083231-4083250 None:intergenic 55.0%
!! CAATTGATGGTGGTGGTGGG+TGG + chr4_3:4083385-4083404 Msa0625080:three_prime_UTR 55.0%
CCAATGCTCTCAGGCCAGAT+TGG - chr4_3:4082868-4082887 None:intergenic 55.0%
! CCAATCTGGCCTGAGAGCAT+TGG + chr4_3:4082865-4082884 Msa0625080:CDS 55.0%
TGTCCCCCGATTTCCTTCGA+TGG + chr4_3:4082969-4082988 Msa0625080:CDS 55.0%
CCCCGATTTCCTTCGATGGT+TGG + chr4_3:4082973-4082992 Msa0625080:CDS 55.0%
TTTGACGACCGGATGCCAGT+GGG + chr4_3:4083220-4083239 Msa0625080:CDS 55.0%
ACATTTCGCCCACTGGCATC+CGG - chr4_3:4083231-4083250 None:intergenic 55.0%
!! CAATTGATGGTGGTGGTGGG+TGG + chr4_3:4083385-4083404 Msa0625080:three_prime_UTR 55.0%
GTCGTCAAAGGCTCAGGAGG+AGG - chr4_3:4083209-4083228 None:intergenic 60.0%
CCTCCTGAGCCTTTGACGAC+CGG + chr4_3:4083209-4083228 Msa0625080:CDS 60.0%
CATCCGGTCGTCAAAGGCTC+AGG - chr4_3:4083215-4083234 None:intergenic 60.0%
CACTGGCATCCGGTCGTCAA+AGG - chr4_3:4083221-4083240 None:intergenic 60.0%
CTTTGACGACCGGATGCCAG+TGG + chr4_3:4083219-4083238 Msa0625080:CDS 60.0%
GTCGTCAAAGGCTCAGGAGG+AGG - chr4_3:4083209-4083228 None:intergenic 60.0%
CCTCCTGAGCCTTTGACGAC+CGG + chr4_3:4083209-4083228 Msa0625080:CDS 60.0%
CATCCGGTCGTCAAAGGCTC+AGG - chr4_3:4083215-4083234 None:intergenic 60.0%
CACTGGCATCCGGTCGTCAA+AGG - chr4_3:4083221-4083240 None:intergenic 60.0%
CTTTGACGACCGGATGCCAG+TGG + chr4_3:4083219-4083238 Msa0625080:CDS 60.0%
CCGGTCGTCAAAGGCTCAGG+AGG - chr4_3:4083212-4083231 None:intergenic 65.0%
!! TGATGGTGGTGGTGGGTGGG+TGG + chr4_3:4083389-4083408 Msa0625080:three_prime_UTR 65.0%
CCGGTCGTCAAAGGCTCAGG+AGG - chr4_3:4083212-4083231 None:intergenic 65.0%
!! TGATGGTGGTGGTGGGTGGG+TGG + chr4_3:4083389-4083408 Msa0625080:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 4082827 4083675 4082827 ID=Msa0625080;Name=Msa0625080
chr4_3 mRNA 4082827 4083675 4082827 ID=Msa0625080-mRNA-1;Parent=Msa0625080;Name=Msa0625080-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|1|0.5|1|1|1|2|350|136
chr4_3 exon 4082827 4083044 4082827 ID=Msa0625080-mRNA-1:exon:938;Parent=Msa0625080-mRNA-1
chr4_3 exon 4083133 4083675 4083133 ID=Msa0625080-mRNA-1:exon:939;Parent=Msa0625080-mRNA-1
chr4_3 CDS 4082827 4083044 4082827 ID=Msa0625080-mRNA-1:cds;Parent=Msa0625080-mRNA-1
chr4_3 CDS 4083133 4083325 4083133 ID=Msa0625080-mRNA-1:cds;Parent=Msa0625080-mRNA-1
chr4_3 three_prime_UTR 4083326 4083675 4083326 ID=Msa0625080-mRNA-1:three_prime_utr;Parent=Msa0625080-mRNA-1
Gene Sequence

>Msa0625080

ATGCAGAGACATGTGGTTGATTTTTACATCTCTGACTTCCAATCTGGCCTGAGAGCATTGGTTAAAACAGGCTTTGGTGCAAGGGTGACTCCTTATGTTGACGATTCCGTTGTAATTGATGTCAATCCGGAGAATAAAGACATGTCCCCCGATTTCCTTCGATGGTTGGGGAAGAGAAACCTTTCTAGTGATGATCGTATCATGCAGTTAAAAGAAGGGTACATAAAAGAAGGTAGCACGGTTAGCGTAATGGGAGTTGTTCATAGAAATGACAATGTACTAATGATTGTGCCTCCTCCTGAGCCTTTGACGACCGGATGCCAGTGGGCGAAATGTATTTTTCCAGCTAGCCTTGAAGGTATTGTTTTGAGATGCGAAGATACATCAAAGATTGATGTGATTCCAGTGTAG
Protein sequence

>Msa0625080

MQRHVVDFYISDFQSGLRALVKTGFGARVTPYVDDSVVIDVNPENKDMSPDFLRWLGKRNLSSDDRIMQLKEGYIKEGSTVSVMGVVHRNDNVLMIVPPPEPLTTGCQWAKCIFPASLEGIVLRCEDTSKIDVIPV*