AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1440950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1440950 MtrunA17_Chr8g0342651 61.905 126 13 1 103 228 88 178 1.70e-40 139
Msa1440950 MtrunA17_Chr8g0342651 49.351 77 31 2 64 140 149 217 5.22e-11 60.8
Msa1440950 MtrunA17_Chr1g0212341 80.882 68 10 1 158 222 21 88 7.87e-28 105
Msa1440950 MtrunA17_Chr1g0212341 40.722 194 67 6 64 257 65 210 1.51e-14 70.5
Msa1440950 MtrunA17_Chr5g0438531 55.932 59 22 1 104 158 69 127 4.80e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 94 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCATTACTTACATTATTT+TGG 0.125240 tig0029168:+3211 Msa1440950:three_prime_UTR
ATTATGCAATGTTACTTTAT+TGG 0.155712 tig0029168:-3467 None:intergenic
ACGATGATTACCTTTGATTT+TGG 0.169382 tig0029168:-2318 None:intergenic
TGTCATCCTTCAATTATATT+TGG 0.184257 tig0029168:+859 Msa1440950:five_prime_UTR
TTGGATTGTTATGTGATATT+AGG 0.202665 tig0029168:-778 None:intergenic
AGATCTTCGCGAAGCTAAAT+TGG 0.247966 tig0029168:+1410 Msa1440950:CDS
CTATTGAACCGGCTTGATTT+AGG 0.266233 tig0029168:+2858 Msa1440950:three_prime_UTR
GAACACAAGCAGTTCATAAT+TGG 0.276454 tig0029168:-3501 None:intergenic
CTAGTTGAATCAAAGCTTCT+TGG 0.282657 tig0029168:-1932 None:intergenic
GAGGAGTAGTGGTAGCATTT+TGG 0.290072 tig0029168:+2814 Msa1440950:exon
GTCATCCTTCAATTATATTT+GGG 0.305842 tig0029168:+860 Msa1440950:five_prime_UTR
AACTTGCCAAATGCATTATC+AGG 0.326793 tig0029168:+3053 Msa1440950:three_prime_UTR
TCGCTTTCTAAATTTATATT+CGG 0.331894 tig0029168:+1317 Msa1440950:CDS
AATAAAGTAACATTGCATAA+TGG 0.337041 tig0029168:+3469 Msa1440950:three_prime_UTR
ATAAAGTAACATTGCATAAT+GGG 0.338506 tig0029168:+3470 Msa1440950:three_prime_UTR
TGTTAATTGTTTAAAATTGA+AGG 0.345557 tig0029168:-826 None:intergenic
TATGTAACGAAATAATAAAT+TGG 0.353966 tig0029168:-797 None:intergenic
TAACTGAGGGATCTGTTAGA+TGG 0.365103 tig0029168:+3133 Msa1440950:three_prime_UTR
TCCTACTTAACCAGATGCTT+TGG 0.368865 tig0029168:+3189 Msa1440950:three_prime_UTR
CCGAAGGATCATCCATTATA+CGG 0.375242 tig0029168:+1291 Msa1440950:CDS
CTGAAACCTGATAATGCATT+TGG 0.390036 tig0029168:-3059 None:intergenic
TGCGTGTCAACGGGTGCAAC+AGG 0.392472 tig0029168:-2278 None:intergenic
AGGAGAAGAACGAACCATTT+GGG 0.393055 tig0029168:-1984 None:intergenic
AATCAAAGCTTCTTGGGTAA+TGG 0.393707 tig0029168:-1925 None:intergenic
TCTTCCACCTCTCCTGGTGC+AGG 0.394878 tig0029168:-2781 None:intergenic
ATTCGCTGCGCTCACTAGTT+CGG 0.405936 tig0029168:+3366 Msa1440950:three_prime_UTR
AGAAAGCGACAACCGTATAA+TGG 0.406074 tig0029168:-1303 None:intergenic
ACTTGATTTCTCTATTGAAC+CGG 0.410703 tig0029168:+2847 Msa1440950:three_prime_UTR
TTCGACCCAAATATAATTGA+AGG 0.423577 tig0029168:-865 None:intergenic
GGGATATGATAAGGAAGATA+AGG 0.437427 tig0029168:+2732 Msa1440950:CDS
CTACAATTGTGCGTGTCAAC+GGG 0.442078 tig0029168:-2287 None:intergenic
ATGGCAGTGCTTTCCCCAAA+TGG 0.446026 tig0029168:+1970 Msa1440950:CDS
CTTCCACCTCTCCTGGTGCA+GGG 0.450689 tig0029168:-2780 None:intergenic
GTTAACTCTTCCACCTCTCC+TGG 0.451248 tig0029168:-2787 None:intergenic
AGACTAAAATTTCACCTCTC+AGG 0.451846 tig0029168:-1489 None:intergenic
ATATTACAAAATAGACCATC+AGG 0.453264 tig0029168:-3345 None:intergenic
CCTTGCCGCCACCATGGACT+TGG 0.453782 tig0029168:+1263 Msa1440950:exon
CCGTATAATGGATGATCCTT+CGG 0.457729 tig0029168:-1291 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 tig0029168:+1600 Msa1440950:CDS
TAGTTGAATCAAAGCTTCTT+GGG 0.458718 tig0029168:-1931 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 tig0029168:+2594 Msa1440950:CDS
TCACAAATCTTCTTTGCTGC+AGG 0.460278 tig0029168:-1846 None:intergenic
CAAGTCCATGGTGGCGGCAA+GGG 0.461283 tig0029168:-1262 None:intergenic
GGATCAGGAGGGATATGATA+AGG 0.465606 tig0029168:+2723 Msa1440950:intron
CAAAAGAACCAACTGCGAAA+AGG 0.465631 tig0029168:+1599 Msa1440950:CDS
CAAAAGAACCAACTGCGAAA+AGG 0.465631 tig0029168:+2593 Msa1440950:CDS
GGAAGGCCAATCTGATGATA+AGG 0.467371 tig0029168:+2753 Msa1440950:CDS
ATATTCGGACGATGCACTCT+TGG 0.468746 tig0029168:+1332 Msa1440950:CDS
AAGTAATGAACCAAAGCATC+TGG 0.474408 tig0029168:-3199 None:intergenic
GCTACAATTGTGCGTGTCAA+CGG 0.476888 tig0029168:-2288 None:intergenic
AGCCCCTGCACCAGGAGAGG+TGG 0.481468 tig0029168:+2777 Msa1440950:CDS
GGGCTTCCTTATCATCAGAT+TGG 0.481597 tig0029168:-2759 None:intergenic
TTCCACCTCTCCTGGTGCAG+GGG 0.483421 tig0029168:-2779 None:intergenic
CTAGTAGAAAATAATGTAGC+AGG 0.490160 tig0029168:-2987 None:intergenic
ATCATCCACAATATTCAGCC+AGG 0.493124 tig0029168:+967 Msa1440950:five_prime_UTR
GTTATATAAGTACGAAGATA+AGG 0.511157 tig0029168:+1356 Msa1440950:CDS
AGTTAACAGATGAGGAGTAG+TGG 0.515594 tig0029168:+2803 Msa1440950:CDS
GATAAGGAAGCCCCTGCACC+AGG 0.518868 tig0029168:+2769 Msa1440950:CDS
CTGCGAAAAGGGCTAAAGTT+GGG 0.522171 tig0029168:+1611 Msa1440950:CDS
ACGATTGATCCTATCCCTGA+TGG 0.525163 tig0029168:+3330 Msa1440950:three_prime_UTR
GGCAAGTTTAACATGATCGC+AGG 0.528195 tig0029168:-3038 None:intergenic
CAATGAAAGTACCGTAGCCC+TGG 0.537406 tig0029168:-985 None:intergenic
ACCAAAGCATCTGGTTAAGT+AGG 0.541232 tig0029168:-3190 None:intergenic
ACTGCGAAAAGGGCTAAAGT+TGG 0.541934 tig0029168:+1610 Msa1440950:CDS
CCAAGTCCATGGTGGCGGCA+AGG 0.543580 tig0029168:-1263 None:intergenic
AACTGAGGGATCTGTTAGAT+GGG 0.547128 tig0029168:+3134 Msa1440950:three_prime_UTR
CAAAATAGACCATCAGGGAT+AGG 0.548322 tig0029168:-3339 None:intergenic
CAACTCCAAGTCCATGGTGG+CGG 0.554736 tig0029168:-1268 None:intergenic
ACTGCGAAAAGGGCTAAAGT+TGG 0.558971 tig0029168:+2604 Msa1440950:CDS
ACAATATTCAGCCAGGGCTA+CGG 0.567396 tig0029168:+974 Msa1440950:five_prime_UTR
TGGGCAATGACTTCTGTGAA+TGG 0.568132 tig0029168:+3153 Msa1440950:three_prime_UTR
GGAAGCCCCTGCACCAGGAG+AGG 0.576902 tig0029168:+2774 Msa1440950:CDS
GGTGGAAGAGTTAACAGATG+AGG 0.581307 tig0029168:+2795 Msa1440950:CDS
AAAAGGGCTAAAGTTGGGAA+AGG 0.581404 tig0029168:+1616 Msa1440950:CDS
AGAAACCCTTGCCGCCACCA+TGG 0.582020 tig0029168:+1257 Msa1440950:intron
CTTCGGCAACTCCAAGTCCA+TGG 0.589099 tig0029168:-1274 None:intergenic
TATTTATGTTTAGGATCAGG+AGG 0.589442 tig0029168:+2711 Msa1440950:intron
AGCTTTAGCCTAAATCAAGC+CGG 0.591356 tig0029168:-2866 None:intergenic
GTAGCCCTGGCTGAATATTG+TGG 0.592581 tig0029168:-972 None:intergenic
AGCCTTGAAGCCAAAATCAA+AGG 0.601595 tig0029168:+2308 Msa1440950:CDS
CATGGACTTGGAGTTGCCGA+AGG 0.601960 tig0029168:+1275 Msa1440950:exon
CTAGAAATAAGTGACAAAGA+TGG 0.605930 tig0029168:+1951 Msa1440950:CDS
ATTTATGTTTAGGATCAGGA+GGG 0.610871 tig0029168:+2712 Msa1440950:intron
AAAGGGCTAAAGTTGGGAAA+GGG 0.618574 tig0029168:+1617 Msa1440950:CDS
TATGATAAGGAAGATAAGGA+AGG 0.621270 tig0029168:+2736 Msa1440950:CDS
TCATCCACAATATTCAGCCA+GGG 0.650109 tig0029168:+968 Msa1440950:five_prime_UTR
CAGGCATTAGCAATATTCCA+AGG 0.651084 tig0029168:-2968 None:intergenic
ATGATCGCAGGATATAGAAG+AGG 0.655857 tig0029168:-3026 None:intergenic
GGAGAAGAACGAACCATTTG+GGG 0.661729 tig0029168:-1983 None:intergenic
CTGAAGTGAAGAAACCTGAG+AGG 0.670696 tig0029168:+1475 Msa1440950:CDS
CGGCAACTCCAAGTCCATGG+TGG 0.682013 tig0029168:-1271 None:intergenic
TATTACAAAATAGACCATCA+GGG 0.682398 tig0029168:-3344 None:intergenic
ATATAAGTACGAAGATAAGG+TGG 0.691646 tig0029168:+1359 Msa1440950:CDS
CTGAAGTGAAGAAACCCGAG+AGG 0.719762 tig0029168:+2461 Msa1440950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTGTTAGTATTT+AGG + tig0029168:1534-1553 Msa1440950:intron 10.0%
!!! TTAGGTTTATTTATTTATTT+TGG + tig0029168:1552-1571 Msa1440950:intron 10.0%
!!! GTATTTGATTTTATTTAATT+TGG + tig0029168:2406-2425 Msa1440950:intron 10.0%
!! TATGTAACGAAATAATAAAT+TGG - tig0029168:800-819 None:intergenic 15.0%
!!! TGTTAATTGTTTAAAATTGA+AGG - tig0029168:829-848 None:intergenic 15.0%
!!! TATTTATTTTGGAATTTTGT+AGG + tig0029168:1563-1582 Msa1440950:intron 15.0%
!!! AAGTATGTTTTCTTTTAATT+TGG + tig0029168:1671-1690 Msa1440950:intron 15.0%
!!! TTGATAATAATTCCTAATTA+GGG - tig0029168:2357-2376 None:intergenic 15.0%
!!! TTTGATAATAATTCCTAATT+AGG - tig0029168:2358-2377 None:intergenic 15.0%
!! AATATTCACAAACTAAAATT+GGG - tig0029168:2499-2518 None:intergenic 15.0%
!!! TTTAGGTTTATCTATTTTTT+TGG + tig0029168:2546-2565 Msa1440950:intron 15.0%
!!! TTATAACTTTACTTTTGTAT+CGG + tig0029168:3241-3260 Msa1440950:three_prime_UTR 15.0%
!!! TTTAGTTGCTAATTTTTTAT+TGG + tig0029168:3295-3314 Msa1440950:three_prime_UTR 15.0%
!!! TTAGTTGCTAATTTTTTATT+GGG + tig0029168:3296-3315 Msa1440950:three_prime_UTR 15.0%
!!! ATTTTGAAGTGTTATTAACA+AGG - tig0029168:898-917 None:intergenic 20.0%
!! AATCAATTTATGTCGAAAAA+CGG + tig0029168:1123-1142 Msa1440950:intron 20.0%
!! TCGCTTTCTAAATTTATATT+CGG + tig0029168:1317-1336 Msa1440950:CDS 20.0%
!! ATATTCACAAACTAAAATTG+GGG - tig0029168:2498-2517 None:intergenic 20.0%
!! CAATATTCACAAACTAAAAT+TGG - tig0029168:2500-2519 None:intergenic 20.0%
!!! CAATTTTAGTTTGTGAATAT+TGG + tig0029168:2498-2517 Msa1440950:intron 20.0%
!!! CTATTTTTTTGGAATTTTGT+AGG + tig0029168:2557-2576 Msa1440950:intron 20.0%
!!! GTTCATTACTTACATTATTT+TGG + tig0029168:3211-3230 Msa1440950:three_prime_UTR 20.0%
!!! TACTTACATTATTTTGGATT+AGG + tig0029168:3217-3236 Msa1440950:three_prime_UTR 20.0%
!!! TATAACTTTACTTTTGTATC+GGG + tig0029168:3242-3261 Msa1440950:three_prime_UTR 20.0%
!!! ATTATGCAATGTTACTTTAT+TGG - tig0029168:3470-3489 None:intergenic 20.0%
!! AATAAAGTAACATTGCATAA+TGG + tig0029168:3469-3488 Msa1440950:three_prime_UTR 20.0%
!! ATAAAGTAACATTGCATAAT+GGG + tig0029168:3470-3489 Msa1440950:three_prime_UTR 20.0%
! TTGGATTGTTATGTGATATT+AGG - tig0029168:781-800 None:intergenic 25.0%
! TGTCATCCTTCAATTATATT+TGG + tig0029168:859-878 Msa1440950:five_prime_UTR 25.0%
! GTCATCCTTCAATTATATTT+GGG + tig0029168:860-879 Msa1440950:five_prime_UTR 25.0%
! TTCATATTTCGAAAAACCTA+AGG + tig0029168:1091-1110 Msa1440950:intron 25.0%
! AAAACGGTTATTCTTTGTAT+TGG + tig0029168:1139-1158 Msa1440950:intron 25.0%
! GTTATATAAGTACGAAGATA+AGG + tig0029168:1356-1375 Msa1440950:CDS 25.0%
!! TTTTAGTCTGTGAATATTGT+TGG + tig0029168:1503-1522 Msa1440950:intron 25.0%
! GAATCATTTCTTCAAAAACA+TGG - tig0029168:1821-1840 None:intergenic 25.0%
! AAAAATTCTCACAAAAGTCT+TGG + tig0029168:1887-1906 Msa1440950:CDS 25.0%
! TTTCAAATTATTAGCTGTGT+CGG - tig0029168:2051-2070 None:intergenic 25.0%
! ATTACTCATAAACCCTAATT+AGG + tig0029168:2342-2361 Msa1440950:intron 25.0%
!! TCTTGTTTGTGTTAGTATTT+AGG + tig0029168:2523-2542 Msa1440950:intron 25.0%
!! TTGTGTTAGTATTTAGGTTT+AGG + tig0029168:2529-2548 Msa1440950:intron 25.0%
!! CTATATTTATGTTTAGGATC+AGG + tig0029168:2708-2727 Msa1440950:intron 25.0%
!!! ATAACTTTACTTTTGTATCG+GGG + tig0029168:3243-3262 Msa1440950:three_prime_UTR 25.0%
! TATTACAAAATAGACCATCA+GGG - tig0029168:3347-3366 None:intergenic 25.0%
! ATATTACAAAATAGACCATC+AGG - tig0029168:3348-3367 None:intergenic 25.0%
TTCGACCCAAATATAATTGA+AGG - tig0029168:868-887 None:intergenic 30.0%
TTACCTATTTCATTCTGCAT+CGG + tig0029168:1062-1081 Msa1440950:intron 30.0%
!! ATTGATTCGTTTACAACCTT+AGG - tig0029168:1110-1129 None:intergenic 30.0%
ATATAAGTACGAAGATAAGG+TGG + tig0029168:1359-1378 Msa1440950:CDS 30.0%
TACCAAACACAATTCATAGA+AGG - tig0029168:1697-1716 None:intergenic 30.0%
TAGTTGAATCAAAGCTTCTT+GGG - tig0029168:1934-1953 None:intergenic 30.0%
CTAGAAATAAGTGACAAAGA+TGG + tig0029168:1951-1970 Msa1440950:CDS 30.0%
TTGAGTGTAATTGTAAGTGT+CGG + tig0029168:2083-2102 Msa1440950:intron 30.0%
!! ACGATGATTACCTTTGATTT+TGG - tig0029168:2321-2340 None:intergenic 30.0%
! AGTTGGAGTATGTTTTCTTT+TGG + tig0029168:2621-2640 Msa1440950:intron 30.0%
! GTTGGAGTATGTTTTCTTTT+GGG + tig0029168:2622-2641 Msa1440950:intron 30.0%
!! CTGGTGCTATATTTATGTTT+AGG + tig0029168:2702-2721 Msa1440950:intron 30.0%
! TATTTATGTTTAGGATCAGG+AGG + tig0029168:2711-2730 Msa1440950:intron 30.0%
! ATTTATGTTTAGGATCAGGA+GGG + tig0029168:2712-2731 Msa1440950:intron 30.0%
TATGATAAGGAAGATAAGGA+AGG + tig0029168:2736-2755 Msa1440950:CDS 30.0%
ACTTGATTTCTCTATTGAAC+CGG + tig0029168:2847-2866 Msa1440950:three_prime_UTR 30.0%
CTAGTAGAAAATAATGTAGC+AGG - tig0029168:2990-3009 None:intergenic 30.0%
! TTCTTTTCTGCATGTTTACT+AGG + tig0029168:3099-3118 Msa1440950:three_prime_UTR 30.0%
! TCTTTTCTGCATGTTTACTA+GGG + tig0029168:3100-3119 Msa1440950:three_prime_UTR 30.0%
AGACTAAAATTTCACCTCTC+AGG - tig0029168:1492-1511 None:intergenic 35.0%
!! GCAGTTGGTTCTTTTGAATT+TGG - tig0029168:1595-1614 None:intergenic 35.0%
AAAAGAACCAACTGCGAAAA+GGG + tig0029168:1600-1619 Msa1440950:CDS 35.0%
TGTTTAAAAATCGAAGCAGC+AGG + tig0029168:1779-1798 Msa1440950:intron 35.0%
AATCAAAGCTTCTTGGGTAA+TGG - tig0029168:1928-1947 None:intergenic 35.0%
CTAGTTGAATCAAAGCTTCT+TGG - tig0029168:1935-1954 None:intergenic 35.0%
TGAGTGTAATTGTAAGTGTC+GGG + tig0029168:2084-2103 Msa1440950:intron 35.0%
!!! TACCTTTGATTTTGGCTTCA+AGG - tig0029168:2313-2332 None:intergenic 35.0%
!! GCAGTTGGTTCTTTTGAATT+TGG - tig0029168:1595-1614 None:intergenic 35.0%
AAAAGAACCAACTGCGAAAA+GGG + tig0029168:1600-1619 Msa1440950:CDS 35.0%
GGGATATGATAAGGAAGATA+AGG + tig0029168:2732-2751 Msa1440950:CDS 35.0%
AACTTGCCAAATGCATTATC+AGG + tig0029168:3053-3072 Msa1440950:three_prime_UTR 35.0%
! CTGAAACCTGATAATGCATT+TGG - tig0029168:3062-3081 None:intergenic 35.0%
! CTTTTCTGCATGTTTACTAG+GGG + tig0029168:3101-3120 Msa1440950:three_prime_UTR 35.0%
! AGGGGAAATTTTCTTAACTG+AGG + tig0029168:3119-3138 Msa1440950:three_prime_UTR 35.0%
! GGGGAAATTTTCTTAACTGA+GGG + tig0029168:3120-3139 Msa1440950:three_prime_UTR 35.0%
! TGGTTAAGTAGGATTTTCTC+TGG - tig0029168:3182-3201 None:intergenic 35.0%
! AAGTAATGAACCAAAGCATC+TGG - tig0029168:3202-3221 None:intergenic 35.0%
!!! GGTATTTTAGCTCACTGTTT+TGG + tig0029168:3387-3406 Msa1440950:three_prime_UTR 35.0%
GAACACAAGCAGTTCATAAT+TGG - tig0029168:3504-3523 None:intergenic 35.0%
ATCATCCACAATATTCAGCC+AGG + tig0029168:967-986 Msa1440950:five_prime_UTR 40.0%
TCATCCACAATATTCAGCCA+GGG + tig0029168:968-987 Msa1440950:five_prime_UTR 40.0%
GAACCGATGCAGAATGAAAT+AGG - tig0029168:1068-1087 None:intergenic 40.0%
!! CCGTATAATGGATGATCCTT+CGG - tig0029168:1294-1313 None:intergenic 40.0%
CCGAAGGATCATCCATTATA+CGG + tig0029168:1291-1310 Msa1440950:CDS 40.0%
AGAAAGCGACAACCGTATAA+TGG - tig0029168:1306-1325 None:intergenic 40.0%
AGATCTTCGCGAAGCTAAAT+TGG + tig0029168:1410-1429 Msa1440950:CDS 40.0%
CTGTGAATATTGTTGGACTC+TGG + tig0029168:1510-1529 Msa1440950:intron 40.0%
CAAAAGAACCAACTGCGAAA+AGG + tig0029168:1599-1618 Msa1440950:CDS 40.0%
AAAAGGGCTAAAGTTGGGAA+AGG + tig0029168:1616-1635 Msa1440950:CDS 40.0%
AAAGGGCTAAAGTTGGGAAA+GGG + tig0029168:1617-1636 Msa1440950:CDS 40.0%
GGCCTTCTATGAATTGTGTT+TGG + tig0029168:1692-1711 Msa1440950:intron 40.0%
TTAAAAATCGAAGCAGCAGG+AGG + tig0029168:1782-1801 Msa1440950:intron 40.0%
TCACAAATCTTCTTTGCTGC+AGG - tig0029168:1849-1868 None:intergenic 40.0%
AGGAGAAGAACGAACCATTT+GGG - tig0029168:1987-2006 None:intergenic 40.0%
AAGGAGAAGAACGAACCATT+TGG - tig0029168:1988-2007 None:intergenic 40.0%
! GAGTGTAATTGTAAGTGTCG+GGG + tig0029168:2085-2104 Msa1440950:intron 40.0%
TATGAAGCATGGACACTTCT+CGG - tig0029168:2120-2139 None:intergenic 40.0%
AGCCTTGAAGCCAAAATCAA+AGG + tig0029168:2308-2327 Msa1440950:CDS 40.0%
GGGAAAAAATTTCACCTCTC+GGG - tig0029168:2478-2497 None:intergenic 40.0%
GGGGAAAAAATTTCACCTCT+CGG - tig0029168:2479-2498 None:intergenic 40.0%
CAAAAGAACCAACTGCGAAA+AGG + tig0029168:1599-1618 Msa1440950:CDS 40.0%
!!! TTTGCTCTGTTTTCTGAAGC+TGG + tig0029168:2683-2702 Msa1440950:intron 40.0%
AGTTAACAGATGAGGAGTAG+TGG + tig0029168:2803-2822 Msa1440950:CDS 40.0%
!! CTATTGAACCGGCTTGATTT+AGG + tig0029168:2858-2877 Msa1440950:three_prime_UTR 40.0%
! AGCTTTAGCCTAAATCAAGC+CGG - tig0029168:2869-2888 None:intergenic 40.0%
! ATATCTTTTCGCACTGTCCT+TGG + tig0029168:2951-2970 Msa1440950:three_prime_UTR 40.0%
CAGGCATTAGCAATATTCCA+AGG - tig0029168:2971-2990 None:intergenic 40.0%
ATGATCGCAGGATATAGAAG+AGG - tig0029168:3029-3048 None:intergenic 40.0%
TAACTGAGGGATCTGTTAGA+TGG + tig0029168:3133-3152 Msa1440950:three_prime_UTR 40.0%
AACTGAGGGATCTGTTAGAT+GGG + tig0029168:3134-3153 Msa1440950:three_prime_UTR 40.0%
TCCTACTTAACCAGATGCTT+TGG + tig0029168:3189-3208 Msa1440950:three_prime_UTR 40.0%
! ACCAAAGCATCTGGTTAAGT+AGG - tig0029168:3193-3212 None:intergenic 40.0%
CAAAATAGACCATCAGGGAT+AGG - tig0029168:3342-3361 None:intergenic 40.0%
ACACACACACACAAGTGCTT+TGG + tig0029168:924-943 Msa1440950:five_prime_UTR 45.0%
ACAATATTCAGCCAGGGCTA+CGG + tig0029168:974-993 Msa1440950:five_prime_UTR 45.0%
ATATTCGGACGATGCACTCT+TGG + tig0029168:1332-1351 Msa1440950:CDS 45.0%
CTGAAGTGAAGAAACCTGAG+AGG + tig0029168:1475-1494 Msa1440950:CDS 45.0%
! ACTTTAGCCCTTTTCGCAGT+TGG - tig0029168:1610-1629 None:intergenic 45.0%
ACTGCGAAAAGGGCTAAAGT+TGG + tig0029168:1610-1629 Msa1440950:CDS 45.0%
CTGCGAAAAGGGCTAAAGTT+GGG + tig0029168:1611-1630 Msa1440950:CDS 45.0%
GGAGAAGAACGAACCATTTG+GGG - tig0029168:1986-2005 None:intergenic 45.0%
! TCTGGTGTCAGTAGAAGAGA+AGG - tig0029168:2007-2026 None:intergenic 45.0%
!! TGTAATTGTAAGTGTCGGGG+CGG + tig0029168:2088-2107 Msa1440950:intron 45.0%
!! GAGAAGTGTCCATGCTTCAT+AGG + tig0029168:2119-2138 Msa1440950:intron 45.0%
GATCAGAGTCCTATGAAGCA+TGG - tig0029168:2131-2150 None:intergenic 45.0%
CTACAATTGTGCGTGTCAAC+GGG - tig0029168:2290-2309 None:intergenic 45.0%
GCTACAATTGTGCGTGTCAA+CGG - tig0029168:2291-2310 None:intergenic 45.0%
! ACTTTAGCCCTTTTCGCAGT+TGG - tig0029168:1610-1629 None:intergenic 45.0%
ACTGCGAAAAGGGCTAAAGT+TGG + tig0029168:1610-1629 Msa1440950:CDS 45.0%
GGATCAGGAGGGATATGATA+AGG + tig0029168:2723-2742 Msa1440950:intron 45.0%
GGAAGGCCAATCTGATGATA+AGG + tig0029168:2753-2772 Msa1440950:CDS 45.0%
GGGCTTCCTTATCATCAGAT+TGG - tig0029168:2762-2781 None:intergenic 45.0%
GGTGGAAGAGTTAACAGATG+AGG + tig0029168:2795-2814 Msa1440950:CDS 45.0%
! GAGGAGTAGTGGTAGCATTT+TGG + tig0029168:2814-2833 Msa1440950:exon 45.0%
GGCAAGTTTAACATGATCGC+AGG - tig0029168:3041-3060 None:intergenic 45.0%
TGGGCAATGACTTCTGTGAA+TGG + tig0029168:3153-3172 Msa1440950:three_prime_UTR 45.0%
ACGATTGATCCTATCCCTGA+TGG + tig0029168:3330-3349 Msa1440950:three_prime_UTR 45.0%
GTAGCCCTGGCTGAATATTG+TGG - tig0029168:975-994 None:intergenic 50.0%
!! CAATGAAAGTACCGTAGCCC+TGG - tig0029168:988-1007 None:intergenic 50.0%
ATGGCAGTGCTTTCCCCAAA+TGG + tig0029168:1970-1989 Msa1440950:CDS 50.0%
TACTGACACCAGACACGACA+TGG + tig0029168:2014-2033 Msa1440950:intron 50.0%
GATTGTGTCCATGTCGTGTC+TGG - tig0029168:2025-2044 None:intergenic 50.0%
GCATGGACACTTCTCGGATT+AGG - tig0029168:2114-2133 None:intergenic 50.0%
CTGAAGTGAAGAAACCCGAG+AGG + tig0029168:2461-2480 Msa1440950:CDS 50.0%
GTTAACTCTTCCACCTCTCC+TGG - tig0029168:2790-2809 None:intergenic 50.0%
ATTCGCTGCGCTCACTAGTT+CGG + tig0029168:3366-3385 Msa1440950:three_prime_UTR 50.0%
CAACTCCAAGTCCATGGTGG+CGG - tig0029168:1271-1290 None:intergenic 55.0%
CTTCGGCAACTCCAAGTCCA+TGG - tig0029168:1277-1296 None:intergenic 55.0%
CATGGACTTGGAGTTGCCGA+AGG + tig0029168:1275-1294 Msa1440950:exon 55.0%
AGAAACCCTTGCCGCCACCA+TGG + tig0029168:1257-1276 Msa1440950:intron 60.0%
!! CAAGTCCATGGTGGCGGCAA+GGG - tig0029168:1265-1284 None:intergenic 60.0%
CGGCAACTCCAAGTCCATGG+TGG - tig0029168:1274-1293 None:intergenic 60.0%
TGCGTGTCAACGGGTGCAAC+AGG - tig0029168:2281-2300 None:intergenic 60.0%
GATAAGGAAGCCCCTGCACC+AGG + tig0029168:2769-2788 Msa1440950:CDS 60.0%
TTCCACCTCTCCTGGTGCAG+GGG - tig0029168:2782-2801 None:intergenic 60.0%
CTTCCACCTCTCCTGGTGCA+GGG - tig0029168:2783-2802 None:intergenic 60.0%
TCTTCCACCTCTCCTGGTGC+AGG - tig0029168:2784-2803 None:intergenic 60.0%
!! CCAAGTCCATGGTGGCGGCA+AGG - tig0029168:1266-1285 None:intergenic 65.0%
CCTTGCCGCCACCATGGACT+TGG + tig0029168:1263-1282 Msa1440950:exon 65.0%
GGAAGCCCCTGCACCAGGAG+AGG + tig0029168:2774-2793 Msa1440950:CDS 70.0%
AGCCCCTGCACCAGGAGAGG+TGG + tig0029168:2777-2796 Msa1440950:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0029168 gene 777 3530 777 ID=Msa1440950;Name=Msa1440950
tig0029168 mRNA 777 3530 777 ID=Msa1440950-mRNA-1;Parent=Msa1440950;Name=Msa1440950-mRNA-1;_AED=0.01;_eAED=0.01;_QI=236|0.85|0.87|1|0.85|0.87|8|708|257
tig0029168 exon 777 995 777 ID=Msa1440950-mRNA-1:exon:12545;Parent=Msa1440950-mRNA-1
tig0029168 exon 1259 1496 1259 ID=Msa1440950-mRNA-1:exon:12546;Parent=Msa1440950-mRNA-1
tig0029168 exon 1585 1672 1585 ID=Msa1440950-mRNA-1:exon:12547;Parent=Msa1440950-mRNA-1
tig0029168 exon 1795 1991 1795 ID=Msa1440950-mRNA-1:exon:12548;Parent=Msa1440950-mRNA-1
tig0029168 exon 2256 2329 2256 ID=Msa1440950-mRNA-1:exon:12549;Parent=Msa1440950-mRNA-1
tig0029168 exon 2437 2482 2437 ID=Msa1440950-mRNA-1:exon:12550;Parent=Msa1440950-mRNA-1
tig0029168 exon 2579 2627 2579 ID=Msa1440950-mRNA-1:exon:12551;Parent=Msa1440950-mRNA-1
tig0029168 exon 2724 3530 2724 ID=Msa1440950-mRNA-1:exon:12552;Parent=Msa1440950-mRNA-1
tig0029168 five_prime_UTR 777 995 777 ID=Msa1440950-mRNA-1:five_prime_utr;Parent=Msa1440950-mRNA-1
tig0029168 five_prime_UTR 1259 1275 1259 ID=Msa1440950-mRNA-1:five_prime_utr;Parent=Msa1440950-mRNA-1
tig0029168 CDS 1276 1496 1276 ID=Msa1440950-mRNA-1:cds;Parent=Msa1440950-mRNA-1
tig0029168 CDS 1585 1672 1585 ID=Msa1440950-mRNA-1:cds;Parent=Msa1440950-mRNA-1
tig0029168 CDS 1795 1991 1795 ID=Msa1440950-mRNA-1:cds;Parent=Msa1440950-mRNA-1
tig0029168 CDS 2256 2329 2256 ID=Msa1440950-mRNA-1:cds;Parent=Msa1440950-mRNA-1
tig0029168 CDS 2437 2482 2437 ID=Msa1440950-mRNA-1:cds;Parent=Msa1440950-mRNA-1
tig0029168 CDS 2579 2627 2579 ID=Msa1440950-mRNA-1:cds;Parent=Msa1440950-mRNA-1
tig0029168 CDS 2724 2822 2724 ID=Msa1440950-mRNA-1:cds;Parent=Msa1440950-mRNA-1
tig0029168 three_prime_UTR 2823 3530 2823 ID=Msa1440950-mRNA-1:three_prime_utr;Parent=Msa1440950-mRNA-1
Gene Sequence

>Msa1440950

ATGGACTTGGAGTTGCCGAAGGATCATCCATTATACGGTTGTCGCTTTCTAAATTTATATTCGGACGATGCACTCTTGGAGTTATATAAGTACGAAGATAAGGTGGACGCTAAATTTGATCTGTGTCTTTTTGAAGATCTTCGCGAAGCTAAATTGGAAAAAGATGAACAAAATCTTTGTTTAGATTCTGAGCAAGCTTCTGAAGTGAAGAAACCTGAGAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGGAAAGGGAAAAATGTTAATGAATCTAATGCTGAAAATGAACAGCAGGAGGCTTGTGCAGTATACCATGTTTTTGAAGAAATGATTCAGTTACCTGCAGCAAAGAAGATTTGTGATGAGCATAATGTTGATGAAAAAATTCTCACAAAAGTCTTGGAGAATTTGAAGAATTCCATTACCCAAGAAGCTTTGATTCAACTAGAAATAAGTGACAAAGATGGCAGTGCTTTCCCCAAATGTGATGAGAGTAAGAAAGATGTTCCTGTTGCACCCGTTGACACGCACAATTGTAGCCTTGAAGCCAAAATCAAAGATTCTCATGCTGTTGAGCAAGCTGCTGAAGTGAAGAAACCCGAGAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGAGATCAGGAGGGATATGATAAGGAAGATAAGGAAGGCCAATCTGATGATAAGGAAGCCCCTGCACCAGGAGAGGTGGAAGAGTTAACAGATGAGGAGTAG
Protein sequence

>Msa1440950

MDLELPKDHPLYGCRFLNLYSDDALLELYKYEDKVDAKFDLCLFEDLREAKLEKDEQNLCLDSEQASEVKKPERKKPNSKEPTAKRAKVGKGKNVNESNAENEQQEACAVYHVFEEMIQLPAAKKICDEHNVDEKILTKVLENLKNSITQEALIQLEISDKDGSAFPKCDESKKDVPVAPVDTHNCSLEAKIKDSHAVEQAAEVKKPERKKPNSKEPTAKRAKVGDQEGYDKEDKEGQSDDKEAPAPGEVEELTDEE*