AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0638580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0638580 MtrunA17_Chr4g0049901 90.196 153 13 2 1 152 88 239 2.51e-90 262
Msa0638580 MtrunA17_Chr5g0412461 41.212 165 81 3 1 150 62 225 4.14e-29 106
Msa0638580 MtrunA17_Chr5g0412271 42.169 166 79 3 1 150 62 226 5.14e-28 103
Msa0638580 MtrunA17_Chr5g0427401 33.939 165 91 3 1 150 62 223 2.32e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0638580 AT2G22540.3 35.393 178 86 3 1 150 62 238 1.58e-21 87.4
Msa0638580 AT2G22540.1 35.393 178 86 3 1 150 62 238 1.58e-21 87.4
Msa0638580 AT2G22540.2 35.393 178 86 3 1 150 57 233 1.75e-21 87.0
Msa0638580 AT4G24540.1 40.252 159 82 6 1 149 62 217 4.98e-21 85.5

Find 45 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCATTGGAATCTGTTATT+AGG 0.132741 4_3:-34091151 Msa0638580:CDS
TGGGGAACAAACCTTTATTT+TGG 0.154101 4_3:+34092327 None:intergenic
TAAGAAGCGCCTCTAGTTTC+TGG 0.185828 4_3:+34092371 None:intergenic
TATACGATATGGTATGTTTA+TGG 0.191406 4_3:-34090589 Msa0638580:three_prime_UTR
TATCATTTACCTTTCTCTTA+AGG 0.249055 4_3:+34092151 None:intergenic
GACAGTATTGCTTCCTTTCT+AGG 0.249592 4_3:-34090792 Msa0638580:three_prime_UTR
TTCAGCAAATCACAGATTAT+TGG 0.255559 4_3:-34092798 Msa0638580:CDS
ATCATTGGAATCTGTTATTA+GGG 0.269501 4_3:-34091150 Msa0638580:CDS
ATCATTTACCTTTCTCTTAA+GGG 0.271180 4_3:+34092152 None:intergenic
GTCTAGTGTTTCCAAAATAA+AGG 0.316806 4_3:-34092338 Msa0638580:intron
GTCGCCATCGTCCATTTATA+AGG 0.317311 4_3:+34091174 None:intergenic
AGTGACACTTCTCTCAAGTT+GGG 0.338517 4_3:-34091095 Msa0638580:CDS
CCAAGTTGATGTATACGATA+TGG 0.348378 4_3:-34090600 Msa0638580:three_prime_UTR
TAGGTACCAGACCTTATAAA+TGG 0.357027 4_3:-34091185 Msa0638580:CDS
TCACTGCCATCGTCGTCTTC+AGG 0.376609 4_3:+34091113 None:intergenic
CTCTCCTTCTCACGGTCCTC+TGG 0.380745 4_3:+34090815 None:intergenic
TGGACGATGGCGACAATCAT+TGG 0.392563 4_3:-34091165 Msa0638580:CDS
TCCATCTACTGATCAGCTTC+AGG 0.394954 4_3:-34092771 Msa0638580:intron
TTACCACATGTTTCAATCTT+CGG 0.404641 4_3:+34091306 None:intergenic
GGTTAAGGAAAATGAGGCCT+TGG 0.425253 4_3:-34090703 Msa0638580:three_prime_UTR
CCATATCGTATACATCAACT+TGG 0.431123 4_3:+34090600 None:intergenic
TAAAGTAATAGATATGGTTA+AGG 0.446785 4_3:-34090718 Msa0638580:three_prime_UTR
GTAATGTAAAGTAATAGATA+TGG 0.448884 4_3:-34090724 Msa0638580:three_prime_UTR
CAAGTAATTGAAAGGCGTAA+TGG 0.461408 4_3:-34092824 Msa0638580:CDS
CAGTGACACTTCTCTCAAGT+TGG 0.471667 4_3:-34091096 Msa0638580:CDS
CATCGTCCATTTATAAGGTC+TGG 0.478047 4_3:+34091179 None:intergenic
CTTCAACACAGGCAATTGAA+TGG 0.479094 4_3:-34092427 Msa0638580:intron
GGTTGATCATCGACCTAGAA+AGG 0.492292 4_3:+34090779 None:intergenic
ACCTGAAGCTGATCAGTAGA+TGG 0.520990 4_3:+34092770 None:intergenic
CATCGACACCCTTAAGAGAA+AGG 0.526872 4_3:-34092160 Msa0638580:intron
AGATATGGTTAAGGAAAATG+AGG 0.554813 4_3:-34090709 Msa0638580:three_prime_UTR
CAGAGGACCGTGAGAAGGAG+AGG 0.556199 4_3:-34090814 Msa0638580:three_prime_UTR
ATAAATAAAATTTCATCCAG+AGG 0.569103 4_3:-34090831 Msa0638580:exon
GTATGGAACAAGTAATTGAA+AGG 0.588861 4_3:-34092832 Msa0638580:CDS
TCATCCAGAGGACCGTGAGA+AGG 0.593867 4_3:-34090819 Msa0638580:three_prime_UTR
GTGACACTTCTCTCAAGTTG+GGG 0.597855 4_3:-34091094 Msa0638580:intron
GAAGCTGATCAGTAGATGGA+TGG 0.615480 4_3:+34092774 None:intergenic
ATATATGTAAACACTTTCTG+TGG 0.627647 4_3:+34090625 None:intergenic
ATACTGTCCTCTCCTTCTCA+CGG 0.636510 4_3:+34090807 None:intergenic
CATATCGTATACATCAACTT+GGG 0.647453 4_3:+34090601 None:intergenic
GAACTACAGTTATACAACCA+AGG 0.666830 4_3:+34090686 None:intergenic
AAGATAAATCTCGTGAACTG+AGG 0.669525 4_3:-34092536 Msa0638580:intron
AAACCGAAGATTGAAACATG+TGG 0.671175 4_3:-34091309 Msa0638580:intron
AGAAGAACTCCAGAAACTAG+AGG 0.688411 4_3:-34092380 Msa0638580:CDS
CAGACCTTATAAATGGACGA+TGG 0.740618 4_3:-34091178 Msa0638580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATGTTAAATATAAAATAA+AGG - chr4_3:34091768-34091787 Msa0638580:intron 10.0%
!! CTATGTTAAATATAAAATAA+AGG - chr4_3:34091768-34091787 Msa0638580:intron 10.0%
!! TTTGTTGGTATAATTTATAA+AGG - chr4_3:34091330-34091349 Msa0638580:CDS 15.0%
!!! ATTTTTAGACAAAAAAGTTA+AGG - chr4_3:34091401-34091420 Msa0638580:intron 15.0%
!!! ATTTTGAATGTTTAAGATTT+AGG - chr4_3:34091825-34091844 Msa0638580:intron 15.0%
!!! TTTTGAATGTTTAAGATTTA+GGG - chr4_3:34091826-34091845 Msa0638580:intron 15.0%
!! ATTCTAAATGTCTATTTATT+GGG + chr4_3:34091914-34091933 None:intergenic 15.0%
!! TATTCTAAATGTCTATTTAT+TGG + chr4_3:34091915-34091934 None:intergenic 15.0%
!!! TTTTTTATACTGGATTTAAT+TGG + chr4_3:34092667-34092686 None:intergenic 15.0%
!! TTTGTTGGTATAATTTATAA+AGG - chr4_3:34091330-34091349 Msa0638580:CDS 15.0%
!!! ATTTTTAGACAAAAAAGTTA+AGG - chr4_3:34091401-34091420 Msa0638580:intron 15.0%
!!! ATTTTGAATGTTTAAGATTT+AGG - chr4_3:34091825-34091844 Msa0638580:intron 15.0%
!!! TTTTGAATGTTTAAGATTTA+GGG - chr4_3:34091826-34091845 Msa0638580:intron 15.0%
!! ATTCTAAATGTCTATTTATT+GGG + chr4_3:34091914-34091933 None:intergenic 15.0%
!! TATTCTAAATGTCTATTTAT+TGG + chr4_3:34091915-34091934 None:intergenic 15.0%
!!! TTTTTTATACTGGATTTAAT+TGG + chr4_3:34092667-34092686 None:intergenic 15.0%
!!! TGATTTTTAAAAAGTATGAG+AGG + chr4_3:34091306-34091325 None:intergenic 20.0%
!! TTTAAAAATCACATCTTTGT+TGG - chr4_3:34091315-34091334 Msa0638580:CDS 20.0%
!!! TCATTCAATTTTATTTGACA+CGG - chr4_3:34091992-34092011 Msa0638580:intron 20.0%
!! TACAATTTGCCATATATATA+TGG - chr4_3:34092165-34092184 Msa0638580:CDS 20.0%
!! ACAATTTGCCATATATATAT+GGG - chr4_3:34092166-34092185 Msa0638580:CDS 20.0%
!!! ATCTCTCAAAAATTTAAGTT+GGG - chr4_3:34092419-34092438 Msa0638580:CDS 20.0%
!! ATAAATAAAATTTCATCCAG+AGG - chr4_3:34092591-34092610 Msa0638580:intron 20.0%
!! GTAATGTAAAGTAATAGATA+TGG - chr4_3:34092698-34092717 Msa0638580:intron 20.0%
!! TAAAGTAATAGATATGGTTA+AGG - chr4_3:34092704-34092723 Msa0638580:intron 20.0%
!!! CAATTTGTTCTATTTTCTTT+TGG - chr4_3:34092765-34092784 Msa0638580:intron 20.0%
!!! TGATTTTTAAAAAGTATGAG+AGG + chr4_3:34091306-34091325 None:intergenic 20.0%
!! TTTAAAAATCACATCTTTGT+TGG - chr4_3:34091315-34091334 Msa0638580:CDS 20.0%
!!! TCATTCAATTTTATTTGACA+CGG - chr4_3:34091992-34092011 Msa0638580:intron 20.0%
!! TACAATTTGCCATATATATA+TGG - chr4_3:34092165-34092184 Msa0638580:CDS 20.0%
!! ACAATTTGCCATATATATAT+GGG - chr4_3:34092166-34092185 Msa0638580:CDS 20.0%
!!! ATCTCTCAAAAATTTAAGTT+GGG - chr4_3:34092419-34092438 Msa0638580:CDS 20.0%
!! ATAAATAAAATTTCATCCAG+AGG - chr4_3:34092591-34092610 Msa0638580:intron 20.0%
!! GTAATGTAAAGTAATAGATA+TGG - chr4_3:34092698-34092717 Msa0638580:intron 20.0%
!! TAAAGTAATAGATATGGTTA+AGG - chr4_3:34092704-34092723 Msa0638580:intron 20.0%
!!! CAATTTGTTCTATTTTCTTT+TGG - chr4_3:34092765-34092784 Msa0638580:intron 20.0%
! TAAAATTCCAGAATCAAAAG+GGG + chr4_3:34090730-34090749 None:intergenic 25.0%
! CTAAAATTCCAGAATCAAAA+GGG + chr4_3:34090731-34090750 None:intergenic 25.0%
!!! AAAAAGGAATGCAACTTTTT+TGG - chr4_3:34090806-34090825 Msa0638580:three_prime_UTR 25.0%
!!! TTTTTTGGACCTTTATTTAC+AGG - chr4_3:34090821-34090840 Msa0638580:three_prime_UTR 25.0%
! GTATATATATGCACCTAATT+AGG + chr4_3:34090933-34090952 None:intergenic 25.0%
! AATACTGAAATCTTCTCATT+TGG - chr4_3:34091150-34091169 Msa0638580:CDS 25.0%
! ATAAGATATACTGATGTTTC+AGG - chr4_3:34091220-34091239 Msa0638580:intron 25.0%
! ATCATTTACCTTTCTCTTAA+GGG + chr4_3:34091273-34091292 None:intergenic 25.0%
! TATCATTTACCTTTCTCTTA+AGG + chr4_3:34091274-34091293 None:intergenic 25.0%
!!! TAAAAAGTATGAGAGGTTTT+TGG + chr4_3:34091299-34091318 None:intergenic 25.0%
! TGGTATAATTTATAAAGGTC+TGG - chr4_3:34091335-34091354 Msa0638580:intron 25.0%
! AGAAACAAAAGAAGTTAACA+TGG + chr4_3:34091568-34091587 None:intergenic 25.0%
! TTTGATTATATTTACCACAC+AGG - chr4_3:34092071-34092090 Msa0638580:intron 25.0%
! TTTATCGACCCATATATATA+TGG + chr4_3:34092177-34092196 None:intergenic 25.0%
!! ATGGAATACATGTGAATTTT+AGG - chr4_3:34092218-34092237 Msa0638580:intron 25.0%
! AATCATTGGAATCTGTTATT+AGG - chr4_3:34092271-34092290 Msa0638580:intron 25.0%
! ATCATTGGAATCTGTTATTA+GGG - chr4_3:34092272-34092291 Msa0638580:intron 25.0%
! AAGCATTACATGATAGTTAA+AGG + chr4_3:34092366-34092385 None:intergenic 25.0%
!! TAATGCTTACTTGTTAATGA+AGG - chr4_3:34092378-34092397 Msa0638580:CDS 25.0%
! CATCTCTCAAAAATTTAAGT+TGG - chr4_3:34092418-34092437 Msa0638580:CDS 25.0%
! ATATATGTAAACACTTTCTG+TGG + chr4_3:34092800-34092819 None:intergenic 25.0%
! TATACGATATGGTATGTTTA+TGG - chr4_3:34092833-34092852 Msa0638580:CDS 25.0%
! TAAAATTCCAGAATCAAAAG+GGG + chr4_3:34090730-34090749 None:intergenic 25.0%
! CTAAAATTCCAGAATCAAAA+GGG + chr4_3:34090731-34090750 None:intergenic 25.0%
!!! AAAAAGGAATGCAACTTTTT+TGG - chr4_3:34090806-34090825 Msa0638580:three_prime_UTR 25.0%
!!! TTTTTTGGACCTTTATTTAC+AGG - chr4_3:34090821-34090840 Msa0638580:three_prime_UTR 25.0%
! GTATATATATGCACCTAATT+AGG + chr4_3:34090933-34090952 None:intergenic 25.0%
! AATACTGAAATCTTCTCATT+TGG - chr4_3:34091150-34091169 Msa0638580:CDS 25.0%
! ATAAGATATACTGATGTTTC+AGG - chr4_3:34091220-34091239 Msa0638580:intron 25.0%
! ATCATTTACCTTTCTCTTAA+GGG + chr4_3:34091273-34091292 None:intergenic 25.0%
! TATCATTTACCTTTCTCTTA+AGG + chr4_3:34091274-34091293 None:intergenic 25.0%
!!! TAAAAAGTATGAGAGGTTTT+TGG + chr4_3:34091299-34091318 None:intergenic 25.0%
! TGGTATAATTTATAAAGGTC+TGG - chr4_3:34091335-34091354 Msa0638580:intron 25.0%
! AGAAACAAAAGAAGTTAACA+TGG + chr4_3:34091568-34091587 None:intergenic 25.0%
! TTTGATTATATTTACCACAC+AGG - chr4_3:34092071-34092090 Msa0638580:intron 25.0%
! TTTATCGACCCATATATATA+TGG + chr4_3:34092177-34092196 None:intergenic 25.0%
!! ATGGAATACATGTGAATTTT+AGG - chr4_3:34092218-34092237 Msa0638580:intron 25.0%
! AATCATTGGAATCTGTTATT+AGG - chr4_3:34092271-34092290 Msa0638580:intron 25.0%
! ATCATTGGAATCTGTTATTA+GGG - chr4_3:34092272-34092291 Msa0638580:intron 25.0%
! AAGCATTACATGATAGTTAA+AGG + chr4_3:34092366-34092385 None:intergenic 25.0%
!! TAATGCTTACTTGTTAATGA+AGG - chr4_3:34092378-34092397 Msa0638580:CDS 25.0%
! CATCTCTCAAAAATTTAAGT+TGG - chr4_3:34092418-34092437 Msa0638580:CDS 25.0%
! ATATATGTAAACACTTTCTG+TGG + chr4_3:34092800-34092819 None:intergenic 25.0%
! TATACGATATGGTATGTTTA+TGG - chr4_3:34092833-34092852 Msa0638580:CDS 25.0%
!! ATCATTGCTTGTTTTCAGTA+TGG - chr4_3:34090573-34090592 Msa0638580:three_prime_UTR 30.0%
GTATGGAACAAGTAATTGAA+AGG - chr4_3:34090590-34090609 Msa0638580:three_prime_UTR 30.0%
TTCAGCAAATCACAGATTAT+TGG - chr4_3:34090624-34090643 Msa0638580:three_prime_UTR 30.0%
GCTAAAATTCCAGAATCAAA+AGG + chr4_3:34090732-34090751 None:intergenic 30.0%
GATTGTGGTTGACATTAATT+TGG - chr4_3:34090776-34090795 Msa0638580:three_prime_UTR 30.0%
! TTAATTTGGTAGCCACAAAA+AGG - chr4_3:34090790-34090809 Msa0638580:three_prime_UTR 30.0%
! AAAAGTTGCATTCCTTTTTG+TGG + chr4_3:34090805-34090824 None:intergenic 30.0%
! GAGTACTTTTAGTCCTAATT+AGG - chr4_3:34090917-34090936 Msa0638580:intron 30.0%
GTCTAGTGTTTCCAAAATAA+AGG - chr4_3:34091084-34091103 Msa0638580:intron 30.0%
TATCTTATATGTCAGAAGCA+TGG + chr4_3:34091208-34091227 None:intergenic 30.0%
! GATATTTTTCCTCCTTATCA+GGG + chr4_3:34091517-34091536 None:intergenic 30.0%
AACATGGAAAACTCCAAAAT+TGG + chr4_3:34091552-34091571 None:intergenic 30.0%
ATTGTTAGATAAGACAGCAT+GGG + chr4_3:34091597-34091616 None:intergenic 30.0%
TATTGTTAGATAAGACAGCA+TGG + chr4_3:34091598-34091617 None:intergenic 30.0%
TATACTTGATCCAAACACAA+TGG + chr4_3:34091659-34091678 None:intergenic 30.0%
AGTGAGTGTAATTGACTAAT+TGG - chr4_3:34091724-34091743 Msa0638580:intron 30.0%
! TGAATGTTTAAGATTTAGGG+TGG - chr4_3:34091829-34091848 Msa0638580:intron 30.0%
! GACATTTAGAATAAATGCAG+TGG - chr4_3:34091922-34091941 Msa0638580:intron 30.0%
! ATTTAGAATAAATGCAGTGG+TGG - chr4_3:34091925-34091944 Msa0638580:intron 30.0%
TTACCACATGTTTCAATCTT+CGG + chr4_3:34092119-34092138 None:intergenic 30.0%
AGAAACAGAAAAACTTTCTG+AGG + chr4_3:34092506-34092525 None:intergenic 30.0%
AGATATGGTTAAGGAAAATG+AGG - chr4_3:34092713-34092732 Msa0638580:intron 30.0%
CATATCGTATACATCAACTT+GGG + chr4_3:34092824-34092843 None:intergenic 30.0%
!! ATCATTGCTTGTTTTCAGTA+TGG - chr4_3:34090573-34090592 Msa0638580:three_prime_UTR 30.0%
GTATGGAACAAGTAATTGAA+AGG - chr4_3:34090590-34090609 Msa0638580:three_prime_UTR 30.0%
TTCAGCAAATCACAGATTAT+TGG - chr4_3:34090624-34090643 Msa0638580:three_prime_UTR 30.0%
GCTAAAATTCCAGAATCAAA+AGG + chr4_3:34090732-34090751 None:intergenic 30.0%
GATTGTGGTTGACATTAATT+TGG - chr4_3:34090776-34090795 Msa0638580:three_prime_UTR 30.0%
! TTAATTTGGTAGCCACAAAA+AGG - chr4_3:34090790-34090809 Msa0638580:three_prime_UTR 30.0%
! AAAAGTTGCATTCCTTTTTG+TGG + chr4_3:34090805-34090824 None:intergenic 30.0%
! GAGTACTTTTAGTCCTAATT+AGG - chr4_3:34090917-34090936 Msa0638580:intron 30.0%
GTCTAGTGTTTCCAAAATAA+AGG - chr4_3:34091084-34091103 Msa0638580:intron 30.0%
TATCTTATATGTCAGAAGCA+TGG + chr4_3:34091208-34091227 None:intergenic 30.0%
! GATATTTTTCCTCCTTATCA+GGG + chr4_3:34091517-34091536 None:intergenic 30.0%
AACATGGAAAACTCCAAAAT+TGG + chr4_3:34091552-34091571 None:intergenic 30.0%
ATTGTTAGATAAGACAGCAT+GGG + chr4_3:34091597-34091616 None:intergenic 30.0%
TATTGTTAGATAAGACAGCA+TGG + chr4_3:34091598-34091617 None:intergenic 30.0%
TATACTTGATCCAAACACAA+TGG + chr4_3:34091659-34091678 None:intergenic 30.0%
AGTGAGTGTAATTGACTAAT+TGG - chr4_3:34091724-34091743 Msa0638580:intron 30.0%
! TGAATGTTTAAGATTTAGGG+TGG - chr4_3:34091829-34091848 Msa0638580:intron 30.0%
! GACATTTAGAATAAATGCAG+TGG - chr4_3:34091922-34091941 Msa0638580:intron 30.0%
! ATTTAGAATAAATGCAGTGG+TGG - chr4_3:34091925-34091944 Msa0638580:intron 30.0%
TTACCACATGTTTCAATCTT+CGG + chr4_3:34092119-34092138 None:intergenic 30.0%
AGAAACAGAAAAACTTTCTG+AGG + chr4_3:34092506-34092525 None:intergenic 30.0%
AGATATGGTTAAGGAAAATG+AGG - chr4_3:34092713-34092732 Msa0638580:intron 30.0%
CATATCGTATACATCAACTT+GGG + chr4_3:34092824-34092843 None:intergenic 30.0%
! CAAGTAATTGAAAGGCGTAA+TGG - chr4_3:34090598-34090617 Msa0638580:three_prime_UTR 35.0%
!! ATGAAAACCCCTTTTGATTC+TGG - chr4_3:34090720-34090739 Msa0638580:three_prime_UTR 35.0%
CTAGTTATTCTGCATGATTG+TGG - chr4_3:34090761-34090780 Msa0638580:three_prime_UTR 35.0%
AAGATAAATCTCGTGAACTG+AGG - chr4_3:34090886-34090905 Msa0638580:intron 35.0%
!! TGGGGAACAAACCTTTATTT+TGG + chr4_3:34091098-34091117 None:intergenic 35.0%
ATTAGTGCACGATTAATCTG+GGG + chr4_3:34091116-34091135 None:intergenic 35.0%
GATTAGTGCACGATTAATCT+GGG + chr4_3:34091117-34091136 None:intergenic 35.0%
AGATTAGTGCACGATTAATC+TGG + chr4_3:34091118-34091137 None:intergenic 35.0%
AATCTTCTCATTTGGTTGTG+TGG - chr4_3:34091158-34091177 Msa0638580:CDS 35.0%
! TTATATGTCAGAAGCATGGA+TGG + chr4_3:34091204-34091223 None:intergenic 35.0%
ATACTGTTCCAATGTGATGT+TGG - chr4_3:34091473-34091492 Msa0638580:intron 35.0%
! GGATATTTTTCCTCCTTATC+AGG + chr4_3:34091518-34091537 None:intergenic 35.0%
CCAAAATTGGAGAATAACAC+TGG + chr4_3:34091539-34091558 None:intergenic 35.0%
!! CCAGTGTTATTCTCCAATTT+TGG - chr4_3:34091536-34091555 Msa0638580:intron 35.0%
ATAAGAGCCCTAATTCAACT+TGG - chr4_3:34091615-34091634 Msa0638580:intron 35.0%
GATGCATTGTCTTGATCTTT+CGG - chr4_3:34091851-34091870 Msa0638580:intron 35.0%
! CAACAAAGTGCTAGTGATTA+CGG + chr4_3:34091881-34091900 None:intergenic 35.0%
!! ACATTGTAGCAAGAGTGTTT+AGG + chr4_3:34092050-34092069 None:intergenic 35.0%
TATATTTACCACACAGGAAG+TGG - chr4_3:34092077-34092096 Msa0638580:intron 35.0%
AAACCGAAGATTGAAACATG+TGG - chr4_3:34092113-34092132 Msa0638580:intron 35.0%
CGCAGCAGCATAATAATATA+TGG - chr4_3:34092199-34092218 Msa0638580:intron 35.0%
TAGGTACCAGACCTTATAAA+TGG - chr4_3:34092237-34092256 Msa0638580:intron 35.0%
TTTAAGAAATGGTAACCTGC+AGG + chr4_3:34092572-34092591 None:intergenic 35.0%
GAACTACAGTTATACAACCA+AGG + chr4_3:34092739-34092758 None:intergenic 35.0%
CCATATCGTATACATCAACT+TGG + chr4_3:34092825-34092844 None:intergenic 35.0%
!! CCAAGTTGATGTATACGATA+TGG - chr4_3:34092822-34092841 Msa0638580:CDS 35.0%
! CAAGTAATTGAAAGGCGTAA+TGG - chr4_3:34090598-34090617 Msa0638580:three_prime_UTR 35.0%
!! ATGAAAACCCCTTTTGATTC+TGG - chr4_3:34090720-34090739 Msa0638580:three_prime_UTR 35.0%
CTAGTTATTCTGCATGATTG+TGG - chr4_3:34090761-34090780 Msa0638580:three_prime_UTR 35.0%
AAGATAAATCTCGTGAACTG+AGG - chr4_3:34090886-34090905 Msa0638580:intron 35.0%
!! TGGGGAACAAACCTTTATTT+TGG + chr4_3:34091098-34091117 None:intergenic 35.0%
ATTAGTGCACGATTAATCTG+GGG + chr4_3:34091116-34091135 None:intergenic 35.0%
GATTAGTGCACGATTAATCT+GGG + chr4_3:34091117-34091136 None:intergenic 35.0%
AGATTAGTGCACGATTAATC+TGG + chr4_3:34091118-34091137 None:intergenic 35.0%
AATCTTCTCATTTGGTTGTG+TGG - chr4_3:34091158-34091177 Msa0638580:CDS 35.0%
! TTATATGTCAGAAGCATGGA+TGG + chr4_3:34091204-34091223 None:intergenic 35.0%
ATACTGTTCCAATGTGATGT+TGG - chr4_3:34091473-34091492 Msa0638580:intron 35.0%
! GGATATTTTTCCTCCTTATC+AGG + chr4_3:34091518-34091537 None:intergenic 35.0%
CCAAAATTGGAGAATAACAC+TGG + chr4_3:34091539-34091558 None:intergenic 35.0%
!! CCAGTGTTATTCTCCAATTT+TGG - chr4_3:34091536-34091555 Msa0638580:intron 35.0%
ATAAGAGCCCTAATTCAACT+TGG - chr4_3:34091615-34091634 Msa0638580:intron 35.0%
GATGCATTGTCTTGATCTTT+CGG - chr4_3:34091851-34091870 Msa0638580:intron 35.0%
! CAACAAAGTGCTAGTGATTA+CGG + chr4_3:34091881-34091900 None:intergenic 35.0%
!! ACATTGTAGCAAGAGTGTTT+AGG + chr4_3:34092050-34092069 None:intergenic 35.0%
TATATTTACCACACAGGAAG+TGG - chr4_3:34092077-34092096 Msa0638580:intron 35.0%
AAACCGAAGATTGAAACATG+TGG - chr4_3:34092113-34092132 Msa0638580:intron 35.0%
CGCAGCAGCATAATAATATA+TGG - chr4_3:34092199-34092218 Msa0638580:intron 35.0%
TAGGTACCAGACCTTATAAA+TGG - chr4_3:34092237-34092256 Msa0638580:intron 35.0%
TTTAAGAAATGGTAACCTGC+AGG + chr4_3:34092572-34092591 None:intergenic 35.0%
GAACTACAGTTATACAACCA+AGG + chr4_3:34092739-34092758 None:intergenic 35.0%
CCATATCGTATACATCAACT+TGG + chr4_3:34092825-34092844 None:intergenic 35.0%
!! CCAAGTTGATGTATACGATA+TGG - chr4_3:34092822-34092841 Msa0638580:CDS 35.0%
CGCTCTCAACCTGTAAATAA+AGG + chr4_3:34090833-34090852 None:intergenic 40.0%
AGTAATTCCACCTTCAACAC+AGG - chr4_3:34090984-34091003 Msa0638580:intron 40.0%
CATTCAATTGCCTGTGTTGA+AGG + chr4_3:34090997-34091016 None:intergenic 40.0%
CTTCAACACAGGCAATTGAA+TGG - chr4_3:34090995-34091014 Msa0638580:intron 40.0%
AGAAGAACTCCAGAAACTAG+AGG - chr4_3:34091042-34091061 Msa0638580:intron 40.0%
CCACTTAACCAACATCACAT+TGG + chr4_3:34091484-34091503 None:intergenic 40.0%
! CCAATGTGATGTTGGTTAAG+TGG - chr4_3:34091481-34091500 Msa0638580:intron 40.0%
! TCCCTCACCAAGTTGAATTA+GGG + chr4_3:34091625-34091644 None:intergenic 40.0%
! TTCCCTCACCAAGTTGAATT+AGG + chr4_3:34091626-34091645 None:intergenic 40.0%
CATCGTCCATTTATAAGGTC+TGG + chr4_3:34092246-34092265 None:intergenic 40.0%
CAGACCTTATAAATGGACGA+TGG - chr4_3:34092244-34092263 Msa0638580:intron 40.0%
AGTGACACTTCTCTCAAGTT+GGG - chr4_3:34092327-34092346 Msa0638580:intron 40.0%
GACAGTATTGCTTCCTTTCT+AGG - chr4_3:34092630-34092649 Msa0638580:intron 40.0%
CGCTCTCAACCTGTAAATAA+AGG + chr4_3:34090833-34090852 None:intergenic 40.0%
AGTAATTCCACCTTCAACAC+AGG - chr4_3:34090984-34091003 Msa0638580:intron 40.0%
CATTCAATTGCCTGTGTTGA+AGG + chr4_3:34090997-34091016 None:intergenic 40.0%
CTTCAACACAGGCAATTGAA+TGG - chr4_3:34090995-34091014 Msa0638580:intron 40.0%
AGAAGAACTCCAGAAACTAG+AGG - chr4_3:34091042-34091061 Msa0638580:intron 40.0%
CCACTTAACCAACATCACAT+TGG + chr4_3:34091484-34091503 None:intergenic 40.0%
! CCAATGTGATGTTGGTTAAG+TGG - chr4_3:34091481-34091500 Msa0638580:intron 40.0%
! TCCCTCACCAAGTTGAATTA+GGG + chr4_3:34091625-34091644 None:intergenic 40.0%
! TTCCCTCACCAAGTTGAATT+AGG + chr4_3:34091626-34091645 None:intergenic 40.0%
CATCGTCCATTTATAAGGTC+TGG + chr4_3:34092246-34092265 None:intergenic 40.0%
CAGACCTTATAAATGGACGA+TGG - chr4_3:34092244-34092263 Msa0638580:intron 40.0%
AGTGACACTTCTCTCAAGTT+GGG - chr4_3:34092327-34092346 Msa0638580:intron 40.0%
GACAGTATTGCTTCCTTTCT+AGG - chr4_3:34092630-34092649 Msa0638580:intron 40.0%
GAAGCTGATCAGTAGATGGA+TGG + chr4_3:34090651-34090670 None:intergenic 45.0%
TCCATCTACTGATCAGCTTC+AGG - chr4_3:34090651-34090670 Msa0638580:three_prime_UTR 45.0%
ACCTGAAGCTGATCAGTAGA+TGG + chr4_3:34090655-34090674 None:intergenic 45.0%
TCAATTGCCTGTGTTGAAGG+TGG + chr4_3:34090994-34091013 None:intergenic 45.0%
!! TAAGAAGCGCCTCTAGTTTC+TGG + chr4_3:34091054-34091073 None:intergenic 45.0%
! ATGTCAGAAGCATGGATGGA+TGG + chr4_3:34091200-34091219 None:intergenic 45.0%
CATCGACACCCTTAAGAGAA+AGG - chr4_3:34091262-34091281 Msa0638580:intron 45.0%
GGAAGCAATTTGCCCTGATA+AGG - chr4_3:34091502-34091521 Msa0638580:intron 45.0%
AGCAATTTGCCCTGATAAGG+AGG - chr4_3:34091505-34091524 Msa0638580:intron 45.0%
! AGCCCTAATTCAACTTGGTG+AGG - chr4_3:34091620-34091639 Msa0638580:intron 45.0%
! GCCCTAATTCAACTTGGTGA+GGG - chr4_3:34091621-34091640 Msa0638580:intron 45.0%
! TCAACTTGGTGAGGGAATGT+AGG - chr4_3:34091629-34091648 Msa0638580:intron 45.0%
! CAACTTGGTGAGGGAATGTA+GGG - chr4_3:34091630-34091649 Msa0638580:intron 45.0%
! AACTTGGTGAGGGAATGTAG+GGG - chr4_3:34091631-34091650 Msa0638580:intron 45.0%
GTCGCCATCGTCCATTTATA+AGG + chr4_3:34092251-34092270 None:intergenic 45.0%
! TTTTCTCCTGAAGACGACGA+TGG - chr4_3:34092303-34092322 Msa0638580:intron 45.0%
CAGTGACACTTCTCTCAAGT+TGG - chr4_3:34092326-34092345 Msa0638580:intron 45.0%
! GTGACACTTCTCTCAAGTTG+GGG - chr4_3:34092328-34092347 Msa0638580:intron 45.0%
ATACTGTCCTCTCCTTCTCA+CGG + chr4_3:34092618-34092637 None:intergenic 45.0%
GGTTGATCATCGACCTAGAA+AGG + chr4_3:34092646-34092665 None:intergenic 45.0%
GGTTAAGGAAAATGAGGCCT+TGG - chr4_3:34092719-34092738 Msa0638580:intron 45.0%
GAAGCTGATCAGTAGATGGA+TGG + chr4_3:34090651-34090670 None:intergenic 45.0%
TCCATCTACTGATCAGCTTC+AGG - chr4_3:34090651-34090670 Msa0638580:three_prime_UTR 45.0%
ACCTGAAGCTGATCAGTAGA+TGG + chr4_3:34090655-34090674 None:intergenic 45.0%
TCAATTGCCTGTGTTGAAGG+TGG + chr4_3:34090994-34091013 None:intergenic 45.0%
!! TAAGAAGCGCCTCTAGTTTC+TGG + chr4_3:34091054-34091073 None:intergenic 45.0%
! ATGTCAGAAGCATGGATGGA+TGG + chr4_3:34091200-34091219 None:intergenic 45.0%
CATCGACACCCTTAAGAGAA+AGG - chr4_3:34091262-34091281 Msa0638580:intron 45.0%
GGAAGCAATTTGCCCTGATA+AGG - chr4_3:34091502-34091521 Msa0638580:intron 45.0%
AGCAATTTGCCCTGATAAGG+AGG - chr4_3:34091505-34091524 Msa0638580:intron 45.0%
! AGCCCTAATTCAACTTGGTG+AGG - chr4_3:34091620-34091639 Msa0638580:intron 45.0%
! GCCCTAATTCAACTTGGTGA+GGG - chr4_3:34091621-34091640 Msa0638580:intron 45.0%
! TCAACTTGGTGAGGGAATGT+AGG - chr4_3:34091629-34091648 Msa0638580:intron 45.0%
! CAACTTGGTGAGGGAATGTA+GGG - chr4_3:34091630-34091649 Msa0638580:intron 45.0%
! AACTTGGTGAGGGAATGTAG+GGG - chr4_3:34091631-34091650 Msa0638580:intron 45.0%
GTCGCCATCGTCCATTTATA+AGG + chr4_3:34092251-34092270 None:intergenic 45.0%
! TTTTCTCCTGAAGACGACGA+TGG - chr4_3:34092303-34092322 Msa0638580:intron 45.0%
CAGTGACACTTCTCTCAAGT+TGG - chr4_3:34092326-34092345 Msa0638580:intron 45.0%
! GTGACACTTCTCTCAAGTTG+GGG - chr4_3:34092328-34092347 Msa0638580:intron 45.0%
ATACTGTCCTCTCCTTCTCA+CGG + chr4_3:34092618-34092637 None:intergenic 45.0%
GGTTGATCATCGACCTAGAA+AGG + chr4_3:34092646-34092665 None:intergenic 45.0%
GGTTAAGGAAAATGAGGCCT+TGG - chr4_3:34092719-34092738 Msa0638580:intron 45.0%
!!! AATTTTATTTATTTAAGAAA+TGG + chr4_3:34092583-34092602 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTTATAC+TGG + chr4_3:34092677-34092696 None:intergenic 5.0%
!!! AATTTTATTTATTTAAGAAA+TGG + chr4_3:34092583-34092602 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTTATAC+TGG + chr4_3:34092677-34092696 None:intergenic 5.0%
TCTCGTGAACTGAGGTACAG+TGG - chr4_3:34090894-34090913 Msa0638580:intron 50.0%
CTCGTGAACTGAGGTACAGT+GGG - chr4_3:34090895-34090914 Msa0638580:intron 50.0%
AGCATGGATGGATGGATGGA+TGG + chr4_3:34091192-34091211 None:intergenic 50.0%
! CAGAAGCATGGATGGATGGA+TGG + chr4_3:34091196-34091215 None:intergenic 50.0%
! ACTTGGTGAGGGAATGTAGG+GGG - chr4_3:34091632-34091651 Msa0638580:intron 50.0%
TGTAGGGGGACCATTGTGTT+TGG - chr4_3:34091646-34091665 Msa0638580:intron 50.0%
GATCAGTTCCACTTCCTGTG+TGG + chr4_3:34092088-34092107 None:intergenic 50.0%
TGGACGATGGCGACAATCAT+TGG - chr4_3:34092257-34092276 Msa0638580:intron 50.0%
TGAGTTCATCTGTGTCCTGC+AGG - chr4_3:34092554-34092573 Msa0638580:CDS 50.0%
TCTCGTGAACTGAGGTACAG+TGG - chr4_3:34090894-34090913 Msa0638580:intron 50.0%
CTCGTGAACTGAGGTACAGT+GGG - chr4_3:34090895-34090914 Msa0638580:intron 50.0%
AGCATGGATGGATGGATGGA+TGG + chr4_3:34091192-34091211 None:intergenic 50.0%
! CAGAAGCATGGATGGATGGA+TGG + chr4_3:34091196-34091215 None:intergenic 50.0%
! ACTTGGTGAGGGAATGTAGG+GGG - chr4_3:34091632-34091651 Msa0638580:intron 50.0%
TGTAGGGGGACCATTGTGTT+TGG - chr4_3:34091646-34091665 Msa0638580:intron 50.0%
GATCAGTTCCACTTCCTGTG+TGG + chr4_3:34092088-34092107 None:intergenic 50.0%
TGGACGATGGCGACAATCAT+TGG - chr4_3:34092257-34092276 Msa0638580:intron 50.0%
TGAGTTCATCTGTGTCCTGC+AGG - chr4_3:34092554-34092573 Msa0638580:CDS 50.0%
TCACTGCCATCGTCGTCTTC+AGG + chr4_3:34092312-34092331 None:intergenic 55.0%
TCATCCAGAGGACCGTGAGA+AGG - chr4_3:34092603-34092622 Msa0638580:intron 55.0%
TCACTGCCATCGTCGTCTTC+AGG + chr4_3:34092312-34092331 None:intergenic 55.0%
TCATCCAGAGGACCGTGAGA+AGG - chr4_3:34092603-34092622 Msa0638580:intron 55.0%
CTCTCCTTCTCACGGTCCTC+TGG + chr4_3:34092610-34092629 None:intergenic 60.0%
CAGAGGACCGTGAGAAGGAG+AGG - chr4_3:34092608-34092627 Msa0638580:intron 60.0%
CTCTCCTTCTCACGGTCCTC+TGG + chr4_3:34092610-34092629 None:intergenic 60.0%
CAGAGGACCGTGAGAAGGAG+AGG - chr4_3:34092608-34092627 Msa0638580:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 34090556 34092888 34090556 ID=Msa0638580;Name=Msa0638580
chr4_3 mRNA 34090556 34092888 34090556 ID=Msa0638580-mRNA-1;Parent=Msa0638580;Name=Msa0638580-mRNA-1;_AED=0.07;_eAED=0.07;_QI=36|1|1|1|0.83|0.71|7|294|152
chr4_3 exon 34090556 34090868 34090556 ID=Msa0638580-mRNA-1:exon:7799;Parent=Msa0638580-mRNA-1
chr4_3 exon 34091095 34091204 34091095 ID=Msa0638580-mRNA-1:exon:7798;Parent=Msa0638580-mRNA-1
chr4_3 exon 34091310 34091351 34091310 ID=Msa0638580-mRNA-1:exon:7797;Parent=Msa0638580-mRNA-1
chr4_3 exon 34092161 34092202 34092161 ID=Msa0638580-mRNA-1:exon:7796;Parent=Msa0638580-mRNA-1
chr4_3 exon 34092339 34092438 34092339 ID=Msa0638580-mRNA-1:exon:7795;Parent=Msa0638580-mRNA-1
chr4_3 exon 34092537 34092601 34092537 ID=Msa0638580-mRNA-1:exon:7794;Parent=Msa0638580-mRNA-1
chr4_3 exon 34092772 34092888 34092772 ID=Msa0638580-mRNA-1:exon:7793;Parent=Msa0638580-mRNA-1
chr4_3 five_prime_UTR 34092853 34092888 34092853 ID=Msa0638580-mRNA-1:five_prime_utr;Parent=Msa0638580-mRNA-1
chr4_3 CDS 34092772 34092852 34092772 ID=Msa0638580-mRNA-1:cds;Parent=Msa0638580-mRNA-1
chr4_3 CDS 34092537 34092601 34092537 ID=Msa0638580-mRNA-1:cds;Parent=Msa0638580-mRNA-1
chr4_3 CDS 34092339 34092438 34092339 ID=Msa0638580-mRNA-1:cds;Parent=Msa0638580-mRNA-1
chr4_3 CDS 34092161 34092202 34092161 ID=Msa0638580-mRNA-1:cds;Parent=Msa0638580-mRNA-1
chr4_3 CDS 34091310 34091351 34091310 ID=Msa0638580-mRNA-1:cds;Parent=Msa0638580-mRNA-1
chr4_3 CDS 34091095 34091204 34091095 ID=Msa0638580-mRNA-1:cds;Parent=Msa0638580-mRNA-1
chr4_3 CDS 34090850 34090868 34090850 ID=Msa0638580-mRNA-1:cds;Parent=Msa0638580-mRNA-1
chr4_3 three_prime_UTR 34090556 34090849 34090556 ID=Msa0638580-mRNA-1:three_prime_utr;Parent=Msa0638580-mRNA-1
Gene Sequence

>Msa0638580

ATGGAACAAGTAATTGAAAGGCGTAATGGATATTCAGCAAATCACAGATTATTGGACCATCCATCTACTGATCAGCTTCAGGTTGAGAGCGACTCTACTTGCGACACACTGCGCAAGAAATTAGAAGATAAATCTCGTGAACTGAGGCAATTGAATGGAGAAGATCTGCAAGAATTGACAGTAGAAGAACTCCAGAAACTAGAGGCGCTTCTTAAAAGAAGTTTGTCTAGTGTTTCCAAAATAAAGGATGAAATGTTTATGCGAGACATCGACACCCTTAAGAGAAAGGAAGTGGAACTGATCGAAGAAAACCGAAGATTGAAACATGTGGTACCAGACCTTATAAATGGACGATGGCGACAATCATTGGAATCTGTTATTAGGGATTCATCTTTTTCTCCTGAAGACGACGATGGCAGTGACACTTCTCTCAAGTTGGGGTTACCATTTCTTAAATAA
Protein sequence

>Msa0638580

MEQVIERRNGYSANHRLLDHPSTDQLQVESDSTCDTLRKKLEDKSRELRQLNGEDLQELTVEELQKLEALLKRSLSSVSKIKDEMFMRDIDTLKRKEVELIEENRRLKHVVPDLINGRWRQSLESVIRDSSFSPEDDDGSDTSLKLGLPFLK*